Hu sheep rumen regulating agent and preparation method thereof

Abstract

The invention discloses a Hu sheep rumen regulating agent and a preparation method thereof, belongs to feed additives, and particularly relates to a preparation method of the Hu sheep rumen regulating agent. The preparation method specifically comprises the following steps: mixing a plant extract and an alkaline compound, adding a functional additive, and uniformly stirring to obtain the Hu sheep rumen regulating agent, the plant extract comprises cinnamyl aldehyde, carvacrol and tea saponin; the alkaline compound comprises sodium bicarbonate and calcium hydrogen phosphate; the functional additive at least comprises sodium fumarate, sub-glycoside acid chalcone and salvia lactone. The prepared Hu sheep rumen regulating agent not only can effectively inhibit the generation amount of methane in the rumen fermentation process, but also is beneficial to improving the weight gain rate of Hu sheep.

Description

Technical Field

[0001] The present invention relates to the technical field of feed additives, and in particular to a Hu sheep rumen regulating agent and a preparation method thereof. Background Art

[0002] As global warming becomes increasingly severe, methane emissions, a major greenhouse gas, are receiving increasing attention. Methanogens in the rumen of ruminants synthesize methane through reduction reactions, using hydrogen and carbon dioxide produced during feed fermentation as their primary electron donors and acceptors. As a crucial component of the rumen's functional ecosystem, the microbial community not only participates in the host's nutrient digestion and metabolism but is also closely linked to digestive tract health and animal performance. Notably, the rumen microbiome exhibits dynamic changes, with its composition being influenced by multiple factors, including animal developmental stage, dietary nutrient levels, additive use, and feeding management practices. Recent studies have shown that scientific nutritional interventions can effectively regulate the rumen microbiome, thereby optimizing nutrient digestion and metabolism, ultimately improving animal performance. While antibiotic additives such as monensin, widely used in traditional animal husbandry, have some regulatory effects, they also pose an increasing challenge, such as drug resistance. In the context of green animal husbandry, the development of safe and effective new feed additives has become a key focus of current ruminant nutrition research.

[0003] As global warming becomes increasingly severe, controlling methane emissions, the second most potent greenhouse gas after carbon dioxide, has become a crucial issue in addressing climate change. In ruminant production, rumen microorganisms convert organic matter in feed into nutrients such as volatile fatty acids through a complex fermentation process. Simultaneously, methanogens use the hydrogen and carbon dioxide produced during fermentation as substrates to synthesize methane through a reduction reaction. This process not only results in energy loss in feed but also has significant environmental impacts.

[0004] As a highly complex microbial ecosystem, the rumen microbiome interacts closely with the host. These microorganisms not only dominate the digestion and metabolism of feed nutrients but also play a key role in maintaining digestive tract homeostasis, regulating immune function, and influencing animal production performance. With the development of microbiome technologies, researchers have discovered that the rumen microbiome exhibits remarkable plasticity, with its composition, structure, and functional characteristics dynamically regulated by multiple factors, including animal age, genetic background, diet composition, feeding environment, and management practices. Nutritional intervention strategies can be used to manipulate the rumen microbiome structure, thereby optimizing metabolic pathways and improving feed utilization efficiency, ultimately achieving both improved production performance and environmental benefits. However, while antibiotic growth promoters such as monensin, widely used in traditional animal husbandry, can effectively suppress specific microbial populations and improve feed conversion, they have attracted widespread global attention due to the spread of bacterial resistance and drug residues. Driven by the concept of sustainable development, the development of new functional feed additives, such as plant extracts, probiotics, and enzyme preparations, has become a research hotspot in ruminant nutrition and a key breakthrough for industrial upgrading. Summary of the Invention

[0005] The purpose of the present invention is to provide a Hu sheep rumen regulator and a preparation method thereof, which can effectively inhibit the production of methane during the rumen fermentation process of Hu sheep and improve the growth performance of Hu sheep.

[0006] The technical solutions adopted by the present invention to achieve the above-mentioned purpose are: A method for preparing a Hu sheep rumen regulator comprises the following steps: mixing a plant extract and an alkaline compound, adding a functional additive and stirring the mixture evenly to obtain the Hu sheep rumen regulator; the functional additive comprises at least sodium fumarate, chalcone glycoside acid and salvia lactone; the mass ratio of sodium fumarate to chalcone glycoside acid is 1:0.1-0.3, and the mass ratio of sodium fumarate to salvia lactone is 1:0.02-0.2.

[0007] The present invention combines plant extracts, alkaline compounds, and functional additives to produce a Hu sheep rumen regulator, which effectively inhibits methane production during rumen fermentation in the Hu sheep, while also improving the sheep's growth performance. The present invention introduces chalcone glycosides and salvia pyrolactone into the Hu sheep rumen regulator. This may reduce the number of protozoa in the rumen, reduce the amount of hydrogen produced by protozoa during carbohydrate decomposition, and inhibit the methane synthesis pathway, thereby reducing methane production during rumen fermentation in ruminants. At the same time, by weakening the protozoa's phagocytic effect on bacteria, the number of rumen bacteria is increased, promoting the utilization of more bacterial proteins by the host, and improving feed utilization efficiency, thereby improving the growth performance of ruminants.

[0008] Preferably, the plant extract comprises cinnamaldehyde, carvacrol and saponin.

[0009] More preferably, the volume ratio of cinnamaldehyde to carvacrol is 1:1-2.

[0010] More preferably, the usage ratio of cinnamaldehyde to tea saponin is 1 mL: 1-5 g.

[0011] Preferably, the alkaline compound includes sodium bicarbonate and calcium hydrogen phosphate.

[0012] More preferably, the mass ratio of sodium bicarbonate to calcium hydrogen phosphate is 1:1-2.

[0013] Preferably, the ratio of the plant extract to the alkaline compound is 1 mL: 0.2-1 g.

[0014] Preferably, the usage ratio of the plant extract and the functional additive is 1 mL: 0.02-0.1 g.

[0015] Preferably, a method for preparing a Hu sheep rumen regulator comprises the following steps: After the plant extract and the alkaline compound are mixed, functional additives are added and stirred evenly to obtain the Huyang rumen regulator.

[0016] More preferably, the plant extract comprises cinnamaldehyde, carvacrol and saponin.

[0017] More preferably, the volume ratio of cinnamaldehyde to carvacrol is 1:1-2.

[0018] More preferably, the usage ratio of cinnamaldehyde to tea saponin is 1 mL:1-5 g.

[0019] More preferably, the alkaline compound includes sodium bicarbonate and calcium hydrogen phosphate.

[0020] More preferably, the mass ratio of sodium bicarbonate to calcium hydrogen phosphate is 1:1-2.

[0021] More preferably, the functional additive includes at least one of sodium fumarate, chalcone glycosides, salvia pyrolactone, and bisdemethoxycurcumin. The present invention further introduces bisdemethoxycurcumin into the Huyang rumen regulator, potentially inhibiting the growth activity of methanogens and interfering with the methane production pathway, thereby reducing methane production during rumen fermentation. It also optimizes rumen fermentation patterns and improves feed metabolic energy utilization, significantly increasing the weight gain rate of ruminants.

[0022] More preferably, the mass ratio of sodium fumarate to chalcone glycosides is 1:0.1-0.3.

[0023] More preferably, the mass ratio of sodium fumarate to salvia lactone is 1:0.02-0.2.

[0024] More preferably, the mass ratio of sodium fumarate to bisdemethoxycurcumin is 1:0.01-0.08.

[0025] More preferably, the ratio of the plant extract to the alkaline compound is 1 mL: 0.2-1 g.

[0026] More preferably, the usage ratio of the plant extract and the functional additive is 1 mL: 0.02-0.1 g.

[0027] The invention also discloses a Hu sheep rumen regulating agent prepared by the preparation method.

[0028] The invention also discloses the application of the Hu sheep rumen regulator in ruminant breeding.

[0029] The present invention utilizes sodium fumarate, chalcone glycosides, salvia pyrolactone, and bisdemethoxycurcumin as functional additives, which are then compounded with plant extracts and alkaline compounds to produce a Hu sheep rumen regulator. The Hu sheep rumen regulator prepared by the present invention not only effectively inhibits methane production during rumen fermentation in Hu sheep, with a methane yield of 16.7-35.0 mL / g, but also effectively improves the growth performance of Hu sheep, with a weight gain rate of 8.4-32.8%. Therefore, the present invention is a Hu sheep rumen regulator with excellent methane inhibition and growth-promoting properties. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 It is the methane production in rumen fermentation of Hu sheep.

[0031] Figure 2 is the weight gain rate of Hu sheep. DETAILED DESCRIPTION

[0032] The present invention will be further described in detail below in conjunction with specific embodiments. The examples provided are only for illustrating the present invention and are not intended to limit the scope of the present invention. The examples provided below can serve as a guide for further improvements by those skilled in the art and are not intended to limit the present invention in any way.

[0033] The experimental methods in the following examples are conventional methods unless otherwise specified. The materials and reagents used in the following examples are commercially available unless otherwise specified.

[0034] Example 1: A method for preparing a Hu sheep rumen regulator, comprising: After mixing a plant extract and an alkaline compound, a functional additive was added and stirred evenly to obtain a rumen regulator for Hu sheep. The plant extract includes cinnamaldehyde, carvacrol, and tea saponin. The volume ratio of cinnamaldehyde to carvacrol is 1:1, and the dosage ratio of cinnamaldehyde to tea saponin is 1 mL:1. The alkaline compound includes sodium bicarbonate and calcium hydrogen phosphate. The mass ratio of sodium bicarbonate to calcium hydrogen phosphate is 1:1. The functional additive includes sodium fumarate, chalcone glycosides, and salvia lactone. The mass ratio of sodium fumarate to chalcone glycosides is 1:0.3, and the mass ratio of sodium fumarate to salvia lactone is 1:0.2. The dosage ratio of the plant extract to the alkaline compound is 1 mL:0.5 g, and the dosage ratio of the plant extract to the functional additive is 1 mL:0.1 g.

[0035] Example 2: A method for preparing a Hu sheep rumen regulator, comprising: After mixing a plant extract and an alkaline compound, a functional additive was added and stirred evenly to obtain a rumen regulator for Hu sheep. The plant extract includes cinnamaldehyde, carvacrol, and tea saponin. The volume ratio of cinnamaldehyde to carvacrol is 1:1, and the dosage ratio of cinnamaldehyde to tea saponin is 1 mL:1. The alkaline compound includes sodium bicarbonate and calcium hydrogen phosphate. The mass ratio of sodium bicarbonate to calcium hydrogen phosphate is 1:1. The functional additive includes sodium fumarate, chalcone glycosides, and salvia lactone. The mass ratio of sodium fumarate to chalcone glycosides is 1:0.1, and the mass ratio of sodium fumarate to salvia lactone is 1:0.2. The dosage ratio of the plant extract to the alkaline compound is 1 mL:0.5 g, and the dosage ratio of the plant extract to the functional additive is 1 mL:0.1 g.

[0036] Example 3: A method for preparing a Hu sheep rumen regulator, comprising: After mixing a plant extract and an alkaline compound, a functional additive was added and stirred evenly to obtain a rumen regulator for Hu sheep. The plant extract includes cinnamaldehyde, carvacrol, and tea saponin. The volume ratio of cinnamaldehyde to carvacrol is 1:1, and the dosage ratio of cinnamaldehyde to tea saponin is 1 mL:1. The alkaline compound includes sodium bicarbonate and calcium hydrogen phosphate. The mass ratio of sodium bicarbonate to calcium hydrogen phosphate is 1:1. The functional additive includes sodium fumarate, chalcone glycosides, and salvia lactone. The mass ratio of sodium fumarate to chalcone glycosides is 1:0.3, and the mass ratio of sodium fumarate to salvia lactone is 1:0.02. The dosage ratio of the plant extract to the alkaline compound is 1 mL:0.5 g, and the dosage ratio of the plant extract to the functional additive is 1 mL:0.1 g.

[0037] Example 4: A method for preparing a Hu sheep rumen regulator, comprising: After mixing a plant extract and an alkaline compound, a functional additive was added and stirred evenly to obtain a Hu sheep rumen regulator. The plant extract includes cinnamaldehyde, carvacrol, and tea saponin. The volume ratio of cinnamaldehyde to carvacrol is 1:1, and the dosage ratio of cinnamaldehyde to tea saponin is 1 mL:1. The alkaline compound includes sodium bicarbonate and calcium hydrogen phosphate. The mass ratio of sodium bicarbonate to calcium hydrogen phosphate is 1:1. The functional additive includes sodium fumarate, chalcone glycosides, caryophyllide, and bisdemethoxycurcumin. The mass ratio of sodium fumarate to chalcone glycosides is 1:0.3, the mass ratio of sodium fumarate to caryophyllide is 1:0.2, and the mass ratio of sodium fumarate to bisdemethoxycurcumin is 1:0.08. The dosage ratio of the plant extract to the alkaline compound is 1 mL:0.5 g, and the dosage ratio of the plant extract to the functional additive is 1 mL:0.1 g.

[0038] Example 5: A method for preparing a Hu sheep rumen regulator, comprising: After mixing a plant extract and an alkaline compound, a functional additive was added and stirred evenly to obtain a Hu sheep rumen regulator. The plant extract includes cinnamaldehyde, carvacrol, and tea saponin. The volume ratio of cinnamaldehyde to carvacrol is 1:1, and the dosage ratio of cinnamaldehyde to tea saponin is 1 mL:1. The alkaline compound includes sodium bicarbonate and calcium hydrogen phosphate. The mass ratio of sodium bicarbonate to calcium hydrogen phosphate is 1:1. The functional additive includes sodium fumarate, chalcone glycosides, caryophyllide, and bisdemethoxycurcumin. The mass ratio of sodium fumarate to chalcone glycosides is 1:0.3, the mass ratio of sodium fumarate to caryophyllide is 1:0.2, and the mass ratio of sodium fumarate to bisdemethoxycurcumin is 1:0.01. The dosage ratio of the plant extract to the alkaline compound is 1 mL:0.5 g, and the dosage ratio of the plant extract to the functional additive is 1 mL:0.1 g.

[0039] Comparative Example 1: A method for preparing a Hu sheep rumen regulator, comprising: After mixing the plant extract and the alkaline compound, a functional additive is added and stirred evenly to obtain a Hu sheep rumen regulator. The plant extract includes cinnamaldehyde, carvacrol, and tea saponin. The volume ratio of cinnamaldehyde to carvacrol is 1:1, and the dosage ratio of cinnamaldehyde to tea saponin is 1mL:1.5g. The alkaline compound includes sodium bicarbonate and calcium hydrogen phosphate. The mass ratio of sodium bicarbonate to calcium hydrogen phosphate is 1:1. The functional additive includes sodium fumarate. The dosage ratio of the plant extract to the alkaline compound is 1mL:0.5g, and the dosage ratio of the plant extract to the functional additive is 1mL:0.1g.

[0040] Comparative Example 2: A method for preparing a Hu sheep rumen regulator, comprising: After mixing the plant extract and the alkaline compound, a functional additive was added and stirred evenly to obtain a Hu sheep rumen regulator. The plant extract includes cinnamaldehyde, carvacrol, and tea saponin, with the volume ratio of cinnamaldehyde to carvacrol being 1:1, and the dosage ratio of cinnamaldehyde to tea saponin being 1mL:1. The alkaline compound includes sodium bicarbonate and calcium hydrogen phosphate, with the mass ratio of sodium bicarbonate to calcium hydrogen phosphate being 1:1. The functional additive includes sodium fumarate and chalcone glycosides, with the mass ratio of sodium fumarate to chalcone glycosides being 1:0.3. The dosage ratio of the plant extract to the alkaline compound is 1mL:0.5g, and the dosage ratio of the plant extract to the functional additive is 1mL:0.1g.

[0041] Comparative Example 3: A method for preparing a Hu sheep rumen regulator, comprising: After mixing the plant extract and the alkaline compound, a functional additive was added and stirred evenly to obtain a Hu sheep rumen regulator. The plant extract includes cinnamaldehyde, carvacrol, and tea saponin, with the volume ratio of cinnamaldehyde to carvacrol being 1:1, and the dosage ratio of cinnamaldehyde to tea saponin being 1 mL:1. The alkaline compound includes sodium bicarbonate and calcium hydrogen phosphate, with the mass ratio of sodium bicarbonate to calcium hydrogen phosphate being 1:1. The functional additive includes sodium fumarate and salvia lactone, with the mass ratio of sodium fumarate to salvia lactone being 1:0.2. The dosage ratio of the plant extract to the alkaline compound is 1 mL:0.5 g, and the dosage ratio of the plant extract to the functional additive is 1 mL:0.1 g.

[0042] Comparative Example 4: A method for preparing a Hu sheep rumen regulator, comprising: After mixing the plant extract and the alkaline compound, the functional additive is added and stirred evenly to obtain a Hu sheep rumen regulator. The plant extract includes cinnamaldehyde, carvacrol, and tea saponin, with the volume ratio of cinnamaldehyde to carvacrol being 1:1, and the dosage ratio of cinnamaldehyde to tea saponin being 1mL:1. The alkaline compound includes sodium bicarbonate and calcium hydrogen phosphate, with the mass ratio of sodium bicarbonate to calcium hydrogen phosphate being 1:1. The functional additive includes sodium fumarate and bisdemethoxycurcumin, with the mass ratio of sodium fumarate to bisdemethoxycurcumin being 1:0.01. The dosage ratio of the plant extract to the alkaline compound is 1mL:0.5g, and the dosage ratio of the plant extract to the functional additive is 1mL:0.1g.

[0043] Experimental example: 1. Methane production Feed preparation: Corn and chinensis were dried at 65°C, crushed, sieved, and mixed to obtain the sample feed. The mass ratio of corn to chinensis was 1:2.5.

[0044] Rumen fluid collection: 12-month-old male Hu sheep in good health were selected and fed twice daily at a rate of 1200 g / day with free access to water for 15 consecutive days. On the 15th day, rumen fluid was collected from the dorsal caeca of the rumen by siphoning two hours after morning feeding. The collected rumen fluid was immediately stored at 39°C, filtered twice through four layers of gauze, and stored at 39°C. Carbon dioxide was then introduced to obtain rumen fluid.

[0045] Artificial saliva was prepared by mixing 400 mL of distilled water, 0.1 mL of trace element solution, 200 mL of buffer, 200 mL of major element solution, and 1 mL of 0.1% resazurin solution. The mixture was saturated with carbon dioxide gas and heated to 39°C. 40 mL of reducing solution was added, and carbon dioxide was continued to be introduced until the solution turned from light blue to anhydrous. To prepare the trace element solution, 13.2 g of calcium chloride dihydrate, 10 g of manganese chloride tetrahydrate, 1 g of cobalt chloride hexahydrate, and 8 g of ferric oxide hexahydrate were mixed and distilled water was added to 1 L to obtain the trace element solution. To prepare the buffer solution, 4 g of ammonium bicarbonate and 35 g of sodium bicarbonate were mixed and distilled water was added to 1 L to obtain the buffer solution. To prepare the constant element solution, 5.7 g of anhydrous disodium hydrogen phosphate, 6.2 g of anhydrous potassium dihydrogen phosphate, and 0.6 g of magnesium sulfate heptahydrate were mixed and distilled water was added to 1 L to obtain a constant element solution. To prepare the reducing solution, 4 mL of 1 mol / L sodium hydroxide solution, 625 mg of sodium sulfide nonahydrate, and 95 mL of distilled water were mixed to obtain a reducing solution.

[0046] Preparation of microbial culture fluid: Mix rumen fluid and artificial saliva evenly, introduce carbon dioxide, and obtain microbial culture fluid.

[0047] Using a fully automatic artificial rumen simulation system, according to the in vitro gas production method, the effects of the Hu sheep rumen regulating agents prepared in Examples 1-5 and Comparative Examples 1-4 on the methane production of Hu sheep rumen fermentation in vitro were respectively determined. The fully automatic artificial rumen simulation system was purchased from Beijing Tianxiang Feiyu Technology Co., Ltd., and the specific steps are as follows: the feed and the Hu sheep rumen regulating agent were mixed in advance to obtain a sample to be tested, and the mass of the Hu sheep rumen regulating agent was equivalent to 0.01% of the mass of the feed; 1g of the sample to be tested was placed in a fermentation bottle, 60mL of microbial culture medium was added, the bubbles in the incubator were discharged, the incubator was sealed, and it was placed at 39°C for 48h. After the culture was completed, the gas in the fermentation bottle was passed into a gas chromatograph to determine the methane production.

[0048] Figure 1is the methane production in the rumen fermentation of Hu sheep, and S1-S9 correspond to the methane production of Examples 1-5 and Comparative Examples 1-4, respectively. The methane production of Examples 1-3 of the present invention is lower than that of Comparative Example 1 because, in the preparation of the Hu sheep rumen regulator, Examples 1-3 additionally used chalcone glycosides and salvia pyrolactone, while Comparative Example 1 did not use chalcone glycosides and salvia pyrolactone; the methane production of Example 1 is lower than that of Examples 2 and 3 because, in the preparation of the Hu sheep rumen regulator, the amounts of chalcone glycosides and salvia pyrolactone used are different. This shows that the introduction of chalcone glycosides and salvia pyrolactone into the Hu sheep rumen regulator of the present invention helps to reduce the methane production in the rumen fermentation of Hu sheep. The methane production in Examples 1-3 of the present invention was lower than that in Comparative Examples 2 and 3 because, in the preparation of the Hu sheep rumen regulator, Examples 1-3 used chalcone glycosides and salvia lactone in combination, while Comparative Example 2 used only chalcone glycosides alone, and Comparative Example 3 used only salvia lactone alone. This indicates that the coordinated use of chalcone glycosides and salvia lactone can effectively reduce the methane production of Hu sheep rumen fermentation compared to the use of chalcone glycosides and salvia lactone alone. The methane production of Examples 4-5 of the present invention is lower than that of Example 1 because, in the preparation of the Hu sheep rumen regulator, Examples 4-5 further use bisdemethoxycurcumin; the methane production of Example 4 is lower than that of Example 5 because, in the preparation of the Hu sheep rumen regulator, the amount of bisdemethoxycurcumin used is different; the methane production of Examples 4-5 is lower than that of Comparative Example 4 because, in the preparation of the Hu sheep rumen regulator, Examples 4-5 further use bisdemethoxycurcumin on the basis of the coordinated use of bisdemethoxychalcone and salvia pyrolactone, while Comparative Example 4 only uses bisdemethoxycurcumin alone. This shows that the further introduction of bisdemethoxycurcumin into the Hu sheep rumen regulator of the present invention helps to further reduce methane production.

[0049] 2. Growth performance Preparation of feed: Corn and chinensis were dried at 65°C, crushed, sieved, and mixed to obtain feed. The mass ratio of corn to chinensis was 1:2.5.

[0050] Preparation of sample feed: The feed and the Huyang rumen regulator are uniformly mixed to obtain a sample feed. The mass of the Huyang rumen regulator is equivalent to 0.01% of the mass of the feed. When the Huyang rumen regulators of Examples 1-5 and Comparative Examples 1-4 are used, the sample feeds of Examples 1-5 and Comparative Examples 1-4 are obtained accordingly.

[0051] Growth performance was measured: 40 male Hu sheep of similar weight and good development were selected and randomly divided into 10 groups, including 1 blank group and 9 experimental groups. The blank group was fed a diet of 1200 g / d twice daily for 15 days with free access to water. The weight was recorded on the 15th day of feeding, recorded as W0. The blank group was then fed a diet of 1200 g / d twice daily for 75 days with free access to water. The weight was recorded after the end of the feeding period, recorded as W1. The nine experimental groups were first fed a uniform diet for 15 days, twice daily at a rate of 1200 g / d, with free access to water. Weight was recorded on the 15th day of feeding, designated as W2. The nine experimental groups were then fed the sample diets of Examples 1-5 and Comparative Examples 1-4, respectively, for 75 days, twice daily at a rate of 1200 g / d, with free access to water. Weight was recorded after the feeding period, designated as W3. The weight gain rate (%) of the Hu sheep in each experimental group was calculated as follows: (W3 - W2) / (W1 - W0) × 100%.

[0052] Figure 2is the weight gain rate of Hu sheep, and S1-S9 correspond to the weight gain rate of Hu sheep in Examples 1-5 and Comparative Examples 1-4, respectively. The weight gain rate of Hu sheep in Examples 1-3 of the present invention is lower than that in Comparative Example 1, because in the preparation of Hu sheep rumen regulator, Examples 1-3 additionally used chalcone glycosides and sage bitter lactone, while Comparative Example 1 did not use chalcone glycosides and sage bitter lactone; the weight gain rate of Hu sheep in Example 1 is lower than that in Examples 2 and 3, because in the preparation of Hu sheep rumen regulator, the amounts of chalcone glycosides and sage bitter lactone used are different. This shows that the introduction of chalcone glycosides and sage bitter lactone into the Hu sheep rumen regulator of the present invention helps to reduce the weight gain rate of Hu sheep caused by rumen fermentation. The weight gain rate of the Hu sheep in Examples 1-3 of the present invention is lower than that in Comparative Examples 2 and 3. This is because, in the preparation of the Hu sheep rumen regulator, Examples 1-3 used chalcone glycosides and salvia lactone in combination, while Comparative Example 2 used only chalcone glycosides alone, and Comparative Example 3 used only salvia lactone alone. This shows that compared with the use of chalcone glycosides and salvia lactone alone, the coordinated use of chalcone glycosides and salvia lactone can effectively reduce the weight gain rate of Hu sheep caused by rumen fermentation. The weight gain rate of the Hu sheep in Examples 4-5 of the present invention is lower than that in Example 1 because, in the preparation of the Hu sheep rumen regulator, Examples 4-5 further use bisdemethoxycurcumin; the weight gain rate of the Hu sheep in Example 4 is lower than that in Example 5 because the amount of bisdemethoxycurcumin used in the preparation of the Hu sheep rumen regulator is different; the weight gain rate of the Hu sheep in Examples 4-5 is lower than that in Comparative Example 4 because, in the preparation of the Hu sheep rumen regulator, Examples 4-5 further use bisdemethoxycurcumin on the basis of the coordinated use of bisdemethoxycurcumin and salvia pyrolactone, while Comparative Example 4 only uses bisdemethoxycurcumin alone. This shows that the further introduction of bisdemethoxycurcumin into the Hu sheep rumen regulator of the present invention helps to further reduce the weight gain rate of the Hu sheep.

[0053] The conventional operations in the operating steps of the present invention are well known to those skilled in the art and will not be described in detail here.

[0054] The embodiments described above provide a detailed description of the technical solutions of the present invention. It should be understood that the above descriptions are only specific embodiments of the present invention and are not intended to limit the present invention. Any changes and modifications made within the scope of the principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A method for preparing a rumen regulator for Hu sheep, comprising: mixing a plant extract and an alkaline compound, adding a functional additive and stirring evenly to obtain the rumen regulator for Hu sheep; the functional additive comprises at least sodium fumarate, chalcone glycoside acid and salvia lactone, the mass ratio of sodium fumarate to chalcone glycoside acid is 1:0.1-0.3, and the mass ratio of sodium fumarate to salvia lactone is 1:0.02-0.

2.

2. The method for preparing a Hu sheep rumen regulating agent according to claim 1, characterized in that: The plant extract includes cinnamaldehyde, carvacrol and tea saponin.

3. The method for preparing a Hu sheep rumen regulating agent according to claim 2, characterized in that: The volume ratio of the cinnamaldehyde to carvacrol is 1:1-2.

4. The method for preparing a Hu sheep rumen regulating agent according to claim 2, characterized in that: The usage ratio of the cinnamaldehyde and the tea saponin is 1 mL: 1-5 g.

5. The method for preparing a Hu sheep rumen regulating agent according to claim 1, characterized in that: The alkaline compounds include sodium bicarbonate and calcium hydrogen phosphate.

6. The method for preparing a Hu sheep rumen regulating agent according to claim 5, characterized in that: The mass ratio of the sodium bicarbonate to calcium hydrogen phosphate is 1:1-2.

7. The method for preparing a Hu sheep rumen regulating agent according to claim 1, characterized in that: The usage ratio of the plant extract and the alkaline compound is 1 mL: 0.2-1 g.

8. The method for preparing a Hu sheep rumen regulating agent according to claim 1, characterized in that: The usage ratio of the plant extract and the functional additive is 1 mL: 0.02-0.1 g.

9. The Hu sheep rumen regulating agent prepared by the preparation method according to any one of claims 1 to 8.

10. Use of the Hu sheep rumen regulator according to claim 9 in ruminant breeding.

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