Premix for improving intestinal health of pets and preparation method thereof

Abstract

The invention belongs to the field of pet food processing, and relates to a premix for improving intestinal health of pets and a preparation method thereof, and the premix comprises ginseng, bighead atractylodes rhizome, liquorice, rhizoma alismatis, hawthorn, lotus seed, Chinese yam, malt and poria cocos. Wherein the ginseng can reinforce vital energy, tonify spleen and benefit stomach; bighead atractylodes rhizome has the effects of invigorating spleen and replenishing qi, eliminating dampness and regulating the middle warmer and is a monarch drug; the poria cocos is sweet and light and has the effects of tonifying spleen and excreting dampness, the Chinese yam and the lotus seed have the effects of tonifying spleen and stopping diarrhea and are ministerial drugs; malt can stimulate appetite and promote digestion and is used for wheaten food stagnation; the rhizoma alismatis is used for clearing damp and promoting diuresis, is used for diarrhea and is an adjuvant drug; the liquorice assists the ginseng, the bighead atractylodes rhizome and the poria cocos in tonifying qi, strengthening the spleen and nourishing the stomach, and can harmonize all the medicines to serve as a conductant medicine. The whole formula is mild in medicine property, and has the effects of tonifying, eliminating and tonifying together to achieve the effects of strengthening the spleen and harmonizing the stomach. The premix disclosed by the invention can be used for improving the water content of excrement, promoting the absorption of intestinal nitrogen, reducing the excretion of nitrogen, increasing the content of acetic acid and propionic acid in the excrement and adjusting the intestinal flora structure, so that the effect of improving the intestinal health is realized.

Description

Technical Field

[0001] The invention belongs to the field of pet food processing and relates to a premix for improving the intestinal health of pets, a preparation method thereof, and an application thereof. Background Art

[0002] Dogs have shorter intestines, with the small intestine located behind the liver and stomach, occupying most of the abdominal cavity. Their large intestine is also very short, similar in diameter to the small intestine, lacking intestinal bands or pouches. Cats have shorter small intestines, underdeveloped cecums, and thicker intestinal walls. This short, wide, and thick intestinal tract is a distinct characteristic of cats, a carnivore.

[0003] The digestive tracts of dogs and cats are generally similar, but each has its own unique characteristics. While the surface area of ​​the mucosa is roughly the same in dogs and cats, the ratio of intestinal mucosal to serous surface area is slightly smaller in dogs than in cats. The ratio of intestinal length to body length is smaller in cats than in dogs, and the ratio of cecum to body weight is slightly larger in dogs than in cats. Furthermore, the terminal small intestine (ileum) of dogs connects directly to the colon, with the cecum and the proximal end of the colon forming the ceco-colic junction. In contrast, the cecum of cats is very small, underdeveloped, and comma-shaped, connecting to the ileum.

[0004] Changes in the intestinal microbiome of dogs and cats can also affect the normal functioning of the body. When an abnormality occurs in the body, the cause is usually the invasion of harmful bacteria into the intestine. In addition, it is also related to the disordered distribution of the number of intestinal microbiome, which affects many important metabolic processes, such as bile acid metabolism and fermentation, amino acid absorption and other processes.

[0005] Gastrointestinal health is crucial for dogs and cats to maintain good health. Maintaining intestinal homeostasis plays a vital role in the health of pets. Intestinal microorganisms regulate host metabolism through digestive, immune, neurological, and endocrine pathways. The intestine is also more prone to problems than other organs. Only by properly regulating the intestine can dogs and cats maintain good health. Summary of the Invention

[0006] In light of this, the present invention aims to provide a premix for improving pet intestinal health and a method for preparing the same. The pet premix can improve fecal moisture content, promote intestinal nitrogen absorption, reduce nitrogen excretion, increase fecal acetic acid and propionic acid content, and adjust the intestinal flora structure, thereby improving intestinal health.

[0007] To achieve the above objectives, this application adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a premix for improving the intestinal health of pets and a preparation method thereof. The pet premix contains the following components in parts by weight: 5-15 parts of ginseng, 5-15 parts of atractylodes macrocephala, 5-15 parts of licorice, 5-15 parts of oriental rhizome, 5-15 parts of hawthorn, 5-15 parts of malt, 15-25 parts of lotus seeds, 15-25 parts of yam, and 15-25 parts of poria.

[0009] In one embodiment of the present invention, the pet premix contains the following components in parts by weight: 10 parts of ginseng, 10 parts of atractylodes macrocephala, 10 parts of licorice, 10 parts of oriental rhizome, 10 parts of hawthorn, 10 parts of malt, 20 parts of lotus seeds, 20 parts of yam, and 20 parts of poria.

[0010] In one embodiment of the present invention, the pet premix contains the following components in parts by weight: 15 parts of ginseng, 15 parts of atractylodes macrocephala, 5 parts of licorice, 5 parts of oriental rhizome, 15 parts of hawthorn, 15 parts of malt, 15 parts of lotus seeds, 15 parts of yam, and 25 parts of poria.

[0011] In one embodiment of the present invention, the pet premix contains the following components in parts by weight: 5 parts of ginseng, 5 parts of atractylodes macrocephala, 15 parts of licorice, 15 parts of oriental rhizome, 5 parts of hawthorn, 5 parts of malt, 25 parts of lotus seeds, 25 parts of yam, and 15 parts of poria.

[0012] In a second aspect, the present invention provides a method for preparing the above-mentioned pet premix, which comprises premixing a formula amount of Chinese herbal medicines including ginseng, atractylodes macrocephala, licorice, oriental Chinese medicine, hawthorn, lotus seeds, yam, malt, and poria, crushing the mixture, and sieving the mixture to obtain a premix for improving the intestinal health of pets.

[0013] In a third aspect, the present invention provides the use of the premix for improving pet intestinal health or the premix for improving pet intestinal health prepared by the above preparation method in preparing pet feed.

[0014] Furthermore, the premix for improving the intestinal health of pets of the present invention is used in the preparation of pet feed for improving the intestinal health.

[0015] The dosage of the premix for improving the intestinal health of pets of the present invention is 1.5-3.5% of the basic diet.

[0016] Furthermore, the dosage of the premix for improving the intestinal health of pets is 2.1% of the basic diet.

[0017] The premix of the present invention contains the following components: ginseng, atractylodes macrocephala, licorice, rhizome of oriental Chinese medicine, hawthorn, lotus seeds, Chinese yam, malt, and poria. Ginseng is a potent invigorator and invigorates the spleen and stomach; atractylodes macrocephala strengthens the spleen and replenishes qi, dispels dampness, and harmonizes the middle, serving as the monarch drug; poria is sweet and mild, invigorates the spleen and eliminates dampness; Chinese yam and lotus seeds invigorate the spleen and stop diarrhea, serving as the assistant drugs; hawthorn eliminates stagnation and dissipates blood stasis, serving as an adjuvant drug for meat stagnation; malt stimulates appetite and helps digestion, serving as an adjuvant drug for wheaten food stagnation; rhizome of oriental Chinese medicine promotes diuresis and eliminates dampness, serving as an adjuvant drug for relieving diarrhea; and licorice assists ginseng, atractylodes macrocephala, and poria in invigorating qi, invigorating the spleen and nourishing the stomach, and also harmonizes the other drugs, serving as the guiding drug. The entire formula has a mild medicinal property, combining both invigoration and elimination, combining elimination with invigoration, and achieving the effects of invigorating the spleen and harmonizing the stomach.

[0018] The beneficial effects achieved by the present invention are:

[0019] The intestinal environment of dogs and cats harbors a diverse microbiome. The intestinal microbiome is composed of bacteria, viruses, and eukaryotic organisms, with bacteria comprising the largest group, providing essential digestive functions such as fiber fermentation. These various microorganisms play an irreplaceable role in the intestine. Lactobacillus and Bifidobacterium were the first probiotics discovered in the animal intestine. These two most common probiotics adhere to the intestinal wall and compete with each other, thickening the intestinal mucosal barrier. They play a key role in regulating intestinal health and microbiome distribution.

[0020] Adding the pet premix of the present invention to the daily diet can improve the water content of feces, promote intestinal nitrogen absorption, reduce nitrogen excretion, increase the content of fecal acetic acid and propionic acid, increase the number of beneficial intestinal bacteria, reduce the number of harmful bacteria, adjust the intestinal flora structure, and thus achieve the effect of improving intestinal health. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the specific implementation methods.

[0022] Figure 1 Comparison of fecal water content in each group, compared with group I, * P<0.05, ** P<0.01; compared with experimental group Ⅱ, # P<0.05, ## P<0.01.

[0023] Figure 2 Comparison of fecal ammonia content in each group and compared with group I, * P<0.05, ** P<0.01; compared with experimental group Ⅱ, # P<0.05, ## P<0.01.

[0024] Figure 3 Comparison of fecal acetic acid content in each group and compared with group I,* P<0.05, ** P<0.01; compared with experimental group Ⅱ, # P<0.05, ## P<0.01.

[0025] Figure 4 Comparison of fecal propionic acid content in each group and compared with group I, * P<0.05, ** P<0.01; compared with experimental group Ⅱ, # P<0.05, ## P<0.01.

[0026] Figure 5 Comparison of fecal E. coli content in each group, compared with group I, * P<0.05, ** P<0.01; compared with experimental group Ⅱ, # P<0.05, ## P<0.01.

[0027] Figure 6 Comparison of the content of Clostridium perfringens in feces of each group and compared with group I, * P<0.05, ** P<0.01; compared with experimental group Ⅱ, # P<0.05. DETAILED DESCRIPTION

[0028] It should be understood that the terms described herein are intended only to describe particular embodiments and are not intended to limit the present invention. In addition, for numerical ranges herein, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Each smaller range between any intermediate value within a stated value or stated range and any other stated value or intermediate value within the stated range is also encompassed by the present invention. The upper and lower limits of these smaller ranges may be independently included or excluded within the scope.

[0029] The raw materials in the present invention are all purchased from commercial sources.

[0030] Example 1

[0031] A premix for improving the intestinal health of pets and a preparation method thereof contain the following components in parts by weight: 10 parts of ginseng, 10 parts of atractylodes macrocephala, 10 parts of liquorice, 10 parts of oriental water chestnuts, 10 parts of hawthorn, 10 parts of malt, 20 parts of lotus seeds, 20 parts of yam, and 20 parts of poria.

[0032] The preparation method comprises the following steps: premixing a formula amount of Chinese herbal medicines including ginseng, atractylodes macrocephala, liquorice, oriental rhizome, hawthorn, lotus seed, yam, malt and poria, crushing the mixture and sieving the mixture to obtain a premix for improving pet intestinal health.

[0033] Example 2

[0034] A premix for improving the intestinal health of pets and a preparation method thereof contain the following components in parts by weight: 15 parts of ginseng, 15 parts of atractylodes macrocephala, 5 parts of liquorice, 5 parts of oriental rhizome, 15 parts of hawthorn, 15 parts of malt, 15 parts of lotus seeds, 15 parts of yam, and 25 parts of poria.

[0035] The preparation method comprises the following steps: premixing a formula amount of Chinese herbal medicines including ginseng, atractylodes macrocephala, liquorice, oriental rhizome, hawthorn, lotus seed, yam, malt and poria, crushing the mixture and sieving the mixture to obtain a premix for improving pet intestinal health.

[0036] Example 3

[0037] A premix for improving the intestinal health of pets and a preparation method thereof contain the following components in parts by weight: 5 parts of ginseng, 5 parts of atractylodes macrocephala, 15 parts of liquorice, 15 parts of oriental water chestnuts, 5 parts of hawthorn, 5 parts of malt, 25 parts of lotus seeds, 25 parts of yam, and 15 parts of poria.

[0038] The preparation method comprises the following steps: premixing a formula amount of Chinese herbal medicines including ginseng, atractylodes macrocephala, liquorice, oriental rhizome, hawthorn, lotus seed, yam, malt and poria, crushing the mixture and sieving the mixture to obtain a premix for improving pet intestinal health.

[0039] Example 4

[0040] A premix for improving the intestinal health of pets and a preparation method thereof contain the following components in parts by weight: 8 parts of ginseng, 5 parts of atractylodes macrocephala, 10 parts of liquorice, 13 parts of oriental radix alisma, 6 parts of hawthorn, 10 parts of malt, 21 parts of lotus seeds, 21 parts of yam, and 15 parts of poria.

[0041] The preparation method comprises the following steps: premixing a formula amount of Chinese herbal medicines including ginseng, atractylodes macrocephala, liquorice, oriental rhizome, hawthorn, lotus seed, yam, malt and poria, crushing the mixture and sieving the mixture to obtain a premix for improving pet intestinal health.

[0042] Example 5

[0043] A premix for improving the intestinal health of pets and a preparation method thereof contain the following components in parts by weight: 15 parts of ginseng, 5 parts of atractylodes macrocephala, 5 parts of liquorice, 5 parts of oriental rhizome, 10 parts of hawthorn, 15 parts of malt, 25 parts of lotus seeds, 20 parts of yam, and 10 parts of poria.

[0044] The preparation method comprises the following steps: premixing a formula amount of Chinese herbal medicines including ginseng, atractylodes macrocephala, liquorice, oriental rhizome, hawthorn, lotus seed, yam, malt and poria, crushing the mixture and sieving the mixture to obtain a premix for improving pet intestinal health.

[0045] Example 6

[0046] A premix for improving the intestinal health of pets and a preparation method thereof contain the following components in parts by weight: 15 parts of ginseng, 5 parts of atractylodes macrocephala, 5 parts of liquorice, 15 parts of oriental water chestnuts, 15 parts of hawthorn, 10 parts of malt, 20 parts of lotus seeds, 20 parts of yam, and 15 parts of poria.

[0047] The preparation method comprises the following steps: premixing a formula amount of Chinese herbal medicines including ginseng, atractylodes macrocephala, liquorice, oriental rhizome, hawthorn, lotus seed, yam, malt and poria, crushing the mixture and sieving the mixture to obtain a premix for improving pet intestinal health.

[0048] Comparative Example 1

[0049] 10 parts of liquorice, 10 parts of rhizoma alismatis, 10 parts of hawthorn, 10 parts of malt, 20 parts of lotus seeds, 20 parts of yam, and 20 parts of poria.

[0050] Compared with Example 1, the difference is that it does not contain ginseng and Atractylodes macrocephala.

[0051] The preparation method is as in Example 1.

[0052] Comparative Example 2

[0053] 10 parts of ginseng, 10 parts of atractylodes macrocephala, 10 parts of liquorice, 10 parts of rhizome of orientalis, 10 parts of hawthorn, 10 parts of malt, and 20 parts of yam.

[0054] Compared with Example 1, the difference is that lotus seeds and Poria cocos are not contained.

[0055] The preparation method is as in Example 1.

[0056] Comparative Example 3

[0057] 10 parts of ginseng, 10 parts of atractylodes macrocephala, 10 parts of liquorice, 10 parts of malt, 20 parts of lotus seeds, 20 parts of yam, and 20 parts of poria.

[0058] Compared with Example 1, the difference is that hawthorn and oriental rhizome are not contained.

[0059] The preparation method is as in Example 1.

[0060] Comparative Example 4

[0061] 10 parts of Codonopsis pilosula, 10 parts of Atractylodes macrocephala, 10 parts of Licorice, 10 parts of Rhizoma Alismatis, 10 parts of Crataegus pinnatifida, 10 parts of Malt, 20 parts of Semen Nelumbo, and 20 parts of Poria.

[0062] Compared with Example 1, the difference is that Codonopsis pilosula is used instead of ginseng, and Chinese yam is not contained.

[0063] The preparation method is as in Example 1.

[0064] Comparative Example 5

[0065] 20 parts of ginseng, 20 parts of atractylodes macrocephala, 3 parts of liquorice, 18 parts of oriental rhizome, 4 parts of hawthorn, 20 parts of malt, 10 parts of lotus seeds, 10 parts of yam, and 5 parts of poria.

[0066] Compared with Example 1, the difference is that the dosage ratio of the raw materials is changed.

[0067] Experimental Example Study on the Effect of the Premix of the Present Invention on Dog Intestinal Health

[0068] Fifty-four healthy beagle dogs aged approximately 2.5 years were randomly divided into nine groups of six dogs each. The dogs were grouped and fed as follows:

[0069] Experimental group I was the control group and was fed a basal diet;

[0070] In the experimental group II, 2.1% of the premix of Example 1 was added to the basal diet;

[0071] In the experimental group III, 2.1% of the premix of Example 2 was added to the basal diet;

[0072] Experimental group IV added 2.1% of the premix of Example 3 to the basal diet;

[0073] In the experimental group V, 2.1% of the premix of comparative example 1 was added to the basal diet;

[0074] In the experimental group VI, 2.1% of the premix of comparative example 2 was added to the basal diet;

[0075] Experimental group VII added 2.1% of the premix of comparative example 3 to the basal diet;

[0076] In the experimental group VIII, 2.1% of the premix of comparative example 4 was added to the basal diet;

[0077] In the experimental group IX, 2.1% of the premix of comparative example 5 was added to the basal diet.

[0078] The basal diets fed to each group were purchased from Keao Xieli (Tianjin) Feed Co., Ltd.

[0079] Before the experiment began, the kennels were cleaned and disinfected, and the food and water bowls were cleaned and disinfected. The kennels were cleaned daily to ensure cleanliness and comfort, and the dogs' mental and health conditions were observed. During the experiment, each dog was housed individually and fed at 8:00 AM daily, with free access to food and water. Food intake was recorded for each dog.

[0080] On the morning of the last day of the experiment, 10 g of fresh feces were collected and placed in a cryotube, immediately frozen in liquid nitrogen, and then stored at -80°C until the sample was tested.

[0081] The moisture content of feces was determined using the oven drying method. The ammonia content of feces was determined using an ammonia meter.

[0082] The content of short-chain fatty acids in dog feces was determined using gas chromatography-mass spectrometry. 30-40 mg of feces was mixed with 1 mL of water. Then, 300 μL of 50% sulfuric acid, 100 μL of 500 mg / L cyclohexanone solution, and 2 mL of ether were added. The mixture was homogenized for 1 minute and then centrifuged at 10,000 x g for 10 minutes at 4°C. The supernatant was collected and analyzed.

[0083] Chromatographic conditions: Agilent DB-WAX capillary column (30 m x 0.25 mm x 0.25 μm); carrier gas: high-purity helium, flow rate: 1.0 mL / min; inlet temperature: 220°C, injection volume: 1 μL, splitless injection, solvent delay time: 2.5 min.

[0084] Weigh 100 mg of feces per sample and extract fecal bacterial DNA from each group according to the instructions provided with the bacterial genomic DNA extraction kit. Real-time fluorescence quantitative PCR reactions were performed on the DNA extracted from the fecal samples to analyze the bacterial species in the feces.

[0085] Data processing

[0086] GraphPad Prism 7.0 software was used for data processing and the quantitative data were The comparison among the groups was performed using one-way analysis of variance, and the difference was considered statistically significant when P < 0.05.

[0087] Test results

[0088] Effects on fecal moisture content

[0089] like Figure 1 As shown, compared with test group I, the fecal water content of test groups II, III, and IV was significantly improved. The effect of improving fecal water content in test groups II, III, and IV was significantly better than that in test groups IV to IX.

[0090] Effects on fecal ammonia levels

[0091] like Figure 2 As shown, compared with Test Group I, the fecal ammonia content of Test Groups II, III, and IV was significantly reduced. The premix of the present invention can also promote intestinal nitrogen absorption and reduce nitrogen excretion. The effect of reducing fecal ammonia content in Test Groups II, III, and IV was significantly better than that in Test Groups IV to IX.

[0092] Effects on short-chain fatty acid content in feces

[0093] like Figure 3 、 Figure 4As shown, compared with test group I, the fecal acetic acid and propionic acid levels in test groups II, III, and IV were significantly increased. The effects of increasing fecal acetic acid and propionic acid levels in test groups II, III, and IV were significantly better than those in test groups IV to IX.

[0094] Effects on fecal intestinal flora

[0095] As shown in Table 1, compared with the test group I, the content of lactobacillus and bifidobacterium in the test groups II, III and IV was significantly increased.

[0096] The effects of increasing the content of Lactobacillus and Bifidobacterium in groups II, III and IV were significantly better than those in groups IV to IX.

[0097] Table 1 Comparison of Lactobacillus and Bifidobacterium contents in each group ( n=6)

[0098]

[0099]

[0100] Note: Compared with the experimental group I, * P<0.05, ** P<0.01; compared with experimental group Ⅱ, # P<0.05, ## P<0.01.

[0101] like Figure 5 、 Figure 6 As shown, compared with test group I, the contents of Escherichia coli and Clostridium perfringens in test groups II, III and IV were significantly reduced.

[0102] The effects of test groups II, III and IV in reducing the content of Escherichia coli and Clostridium perfringens were significantly better than those of test groups IV to IX.

[0103] Under the condition of the same premix dosage, changing the raw materials or the raw material dosage ratio of the premix will weaken the effect of the premix in improving intestinal health. The raw materials of the premix of the present invention synergize with each other and significantly enhance the effect of improving intestinal health.

[0104] Adding the pet premix of the present invention to the diet can improve the water content of feces, promote intestinal nitrogen absorption, reduce nitrogen excretion, increase fecal acetic acid and propionic acid content, adjust the intestinal flora structure, and thus achieve the effect of improving intestinal health.

[0105] The above is only an embodiment of the present invention, and the common knowledge such as the specific technical solutions and / or characteristics in the solution are not described in detail here. It should be pointed out that for those skilled in the art, without departing from the technical solution of the present invention, several variations and improvements can be made, which should also be regarded as the scope of protection of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the description can be used to interpret the content of the claims.

Claims

1. A premix for improving the intestinal health of pets, characterized in that: The pet premix contains the following components in parts by weight: 5-15 parts of ginseng, 5-15 parts of atractylodes macrocephala, 5-15 parts of liquorice, 5-15 parts of oriental rhizome, 5-15 parts of hawthorn, 5-15 parts of malt, 15-25 parts of lotus seeds, 15-25 parts of yam, and 15-25 parts of poria.

2. The premix for improving pet intestinal health according to claim 1, characterized in that: The pet premix contains the following components in parts by weight: 10 parts of ginseng, 10 parts of atractylodes macrocephala, 10 parts of liquorice, 10 parts of oriental rhizome, 10 parts of hawthorn, 10 parts of malt, 20 parts of lotus seeds, 20 parts of yam, and 20 parts of poria.

3. The premix for improving pet intestinal health according to claim 1, characterized in that: The pet premix contains the following components in parts by weight: 15 parts of ginseng, 15 parts of atractylodes macrocephala, 5 parts of liquorice, 5 parts of oriental rhizome, 15 parts of hawthorn, 15 parts of malt, 15 parts of lotus seeds, 15 parts of yam, and 25 parts of poria.

4. The premix for improving pet intestinal health according to claim 1, characterized in that: The pet premix contains the following components in parts by weight: 5 parts of ginseng, 5 parts of atractylodes macrocephala, 15 parts of liquorice, 15 parts of oriental rhizome, 5 parts of hawthorn, 5 parts of malt, 25 parts of lotus seeds, 25 parts of yam, and 15 parts of poria.

5. The premix for improving pet intestinal health according to claim 1, characterized in that: The preparation method comprises the following steps: premixing a formula amount of Chinese herbal medicines including ginseng, atractylodes macrocephala, liquorice, oriental rhizome, hawthorn, lotus seed, yam, malt and poria, crushing the mixture and sieving the mixture to obtain a premix for improving pet intestinal health.

6. Use of the premix for improving pet intestinal health according to any one of claims 1 to 4 or the premix for improving pet intestinal health prepared by the preparation method according to claim 5 in preparing pet feed.

7. The use according to claim 6, characterized in that The pet premix is ​​used in preparing pet feed for improving intestinal health.

8. The use according to claim 6, characterized in that The dosage of the premix for improving the intestinal health of pets is 1.5-3.5% of the basic diet.

9. The use according to claim 8, characterized in that The dosage of the premix for improving the intestinal health of pets is 2.1% of the basic diet.

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