Microbial fermentation feed capable of improving disease resistance of broiler chickens and preparation method of microbial fermentation feed

Abstract

The invention relates to the technical field of livestock and poultry feeds, and discloses a microbial fermentation feed for improving disease resistance of broiler chickens and a preparation method of the microbial fermentation feed. The basic raw materials comprise fermented soybean meal, corn, bran, corn straw powder, rice bran and brewer's grains; the complex microbial inoculants comprise lactobacillus acidophilus, lactobacillus plantarum, bacillus subtilis, candida utilis, bifidobacterium bifidum and rhodopseudomonas palustris; the disease-resistant strengthening additive comprises selenium yeast, a folium cortex eucommiae extract, astragalus polysaccharide, xylooligosaccharide and vitamin E. According to the prepared microbial fermentation feed, through the synergistic mechanism of the complex microbial inoculant and the disease-resistant fortified additive, the nutrition utilization rate and the intestinal barrier function of the broiler chickens are remarkably improved, intestinal diseases are reduced, and the requirement for disease-resistant feed in large-scale breeding of the broiler chickens is met.

Description

Technical Field

[0001] The present invention relates to the technical field of livestock and poultry feed, in particular to a microbial fermented feed capable of improving the disease resistance of broilers and a preparation method thereof. Background Art

[0002] Broiler farming is a pillar of my country's animal husbandry industry, with scale and output ranking among the highest in the world. It plays a crucial role in ensuring the supply of animal protein. Broilers have a short growth cycle and rapid growth rate, but this also makes them susceptible to pathogens and environmental stress, making disease prevention and control a core challenge in the industry. Traditional control methods, which rely on antibiotics, are subject to problems such as drug resistance, drug residues, and ecological pollution.

[0003] As an alternative, microbial fermentation feed has become a research hotspot because it regulates the balance of intestinal microecology through beneficial bacteria and enhances host immunity. In the existing technology, the application effect of a single strain of bacteria or a simple composite bacterial agent (such as only lactic acid bacteria or Bacillus) is limited, and it is difficult to achieve multi-dimensional disease resistance. For example, although a single Bacillus subtilis can improve intestinal morphology, it has a weak effect on mycotoxin degradation and antioxidant capacity enhancement. In addition, conventional fermentation processes are not efficient enough for unconventional raw materials such as corn straw, and the cellulose degradation rate is low, resulting in the nutritional value of the feed not being fully released.

[0004] Currently, there is still room for improvement in research on microbial fermented feed. Therefore, in-depth research and optimization of microbial fermented feed formulas and preparation methods to improve broiler disease resistance are of great significance to promoting antibiotic-free broiler farming and improving industry efficiency. Summary of the Invention

[0005] The purpose of the present invention is to provide a microbial fermented feed that improves the disease resistance of broilers and a preparation method thereof. By designing a composite bacterial agent with synergistic multiple strains, adding disease-resistant enhancing additives, optimizing the raw material segmented pretreatment process and the aerobic-anaerobic segmented fermentation process, the efficient release of nutrients in the feed and the stable proliferation of functional bacteria are achieved, ultimately improving the intestinal health and disease resistance of broilers, and providing a practical technical solution for antibiotic-free breeding of broilers.

[0006] To achieve the above object, the present invention provides a microbial fermented feed for improving disease resistance of broiler chickens, comprising:

[0007] Basic raw materials: fermented soybean meal, corn, bran, corn straw powder, rice bran, brewer's grains;

[0008] Composite bacterial agent: Lactobacillus acidophilus, Lactobacillus plantarum, Bacillus subtilis, Candida utilis, Bifidobacterium bifidum, Rhodopseudomonas palustris;

[0009] Disease-resistant strengthening additives: selenium yeast, eucommia leaf extract, astragalus polysaccharide, oligoxylose, vitamin E.

[0010] Furthermore, the amount of each component is calculated by weight: 18-22 parts of fermented soybean meal, 12-14 parts of corn straw powder, 22-28 parts of corn, 9-11 parts of bran, 6-7 parts of rice bran, 7-9 parts of brewer's grains, 2.2-2.8 parts of Bacillus subtilis, 1.3-1.7 parts of Lactobacillus plantarum, 2.5-3.5 parts of Lactobacillus acidophilus, 0.6-0.8 parts of Bifidobacterium bifidum, 0.6-0.9 parts of Candida utilis, 0.3-0.4 parts of Rhodopseudomonas palustris, 1.2-1.8 parts of Eucommia ulmoides leaf extract, 0.6-0.9 parts of astragalus polysaccharide, 0.22-0.28 parts of selenium yeast, 0.12-0.18 parts of vitamin E, and 0.3-0.5 parts of xylo-oligosaccharide.

[0011] Furthermore, the total viable bacterial count of the composite bacterial agent is ≥1×10 9 CFU / g, of which the number of live bacteria of Bacillus subtilis and Lactobacillus plantarum accounts for ≥50%, and the number of live bacteria of Rhodopseudomonas palustris ≥3×10 7 CFU / g.

[0012] Furthermore, the present invention also provides a method for preparing a microbial fermented feed for improving the disease resistance of broiler chickens, comprising the following steps:

[0013] S1. Corn straw powder pretreatment: corn straw powder was mixed with water, enzymatically hydrolyzed with a complex enzyme, and then aerobic fermentation with Trichoderma reesei was carried out;

[0014] S2, soybean meal-brewery grain pretreatment: fermented soybean meal and brewer's grain are mixed, water is added, and then inoculated with Bacillus subtilis and Candida utilis for aerobic fermentation;

[0015] S3, aerobic stage: all pretreated raw materials are mixed with puffed corn, bran, and rice bran, and inoculated with Bacillus subtilis, Candida utilis, and Rhodopseudomonas palustris for aerobic fermentation;

[0016] S4, anaerobic stage: ventilation is turned off, and Lactobacillus plantarum, Lactobacillus acidophilus, and Bifidobacterium bifidum are introduced for sealed fermentation;

[0017] S5, cooling the anaerobic fermentation product, adding disease-resistant strengthening additives, and allowing it to ferment;

[0018] S6. The fermentation product is vacuum freeze-dried and crushed to obtain microbial fermentation feed.

[0019] Furthermore, in step S1: corn straw powder and water are mixed in a weight ratio of 1:1.5; the complex enzyme is composed of cellulase and xylanase in a weight ratio of 1:1, and the added amount is 0.4% of the weight of the corn straw powder; the enzymatic hydrolysis temperature is 50-55°C, and the enzymatic hydrolysis time is 2-3h; the inoculum amount of Trichoderma reesei is 1.5% of the weight of the corn straw powder, the aerobic fermentation temperature is 30°C, the ventilation volume is 1.5L / (L·min), and the fermentation time is 8h.

[0020] Furthermore, in step S2: fermented soybean meal and brewer's grains are mixed in a weight ratio of 2:1; the amount of water added is 3.5% of the weight of the mixture; the inoculum amount of Bacillus subtilis is 1.5% of the weight of the mixture, and the inoculum amount of Candida utilis is 0.5% of the weight of the mixture; the aerobic fermentation temperature is 30°C, the ventilation volume is 1.5 L / (L·min), and the fermentation time is 8 hours.

[0021] Furthermore, in step S3: the inoculum amount of Bacillus subtilis is 1.0% of the weight of the mixed raw materials, the inoculum amount of Candida utilis is 0.8% of the weight of the mixed raw materials, and the inoculum amount of Rhodopseudomonas palustris is 0.7% of the weight of the mixed raw materials; the moisture content of the material is controlled at 42-45%; the aerobic fermentation temperature is 30°C, the ventilation volume is 1.5 L / (L·min), and the fermentation time is 12 h.

[0022] Furthermore, in step S4: the inoculation amount of Lactobacillus plantarum is 0.8% of the weight of the aerobic fermentation product, the inoculation amount of Lactobacillus acidophilus is 1.0% of the weight of the aerobic fermentation product, and the inoculation amount of Bifidobacterium bifidum is 0.4% of the weight of the aerobic fermentation product; the anaerobic fermentation temperature is 37°C, the fermentation time is 24 hours, and the pH of the material is controlled at 4.6-4.8 during the fermentation process.

[0023] Furthermore, in step S5: after the anaerobic fermentation product is cooled to 30° C., an anti-disease strengthening additive is added; the fermentation temperature is allowed to stand at 30° C. for 3-4 hours, and the container is kept sealed during the standing process.

[0024] Furthermore, in step S6: the freeze-drying temperature is -40 to -35°C, and the moisture content of the material after drying is ≤7%; and the material is sieved through a 40-mesh sieve after being crushed.

[0025] Therefore, the advantages and positive effects of the microbial fermented feed for improving the disease resistance of broilers and the preparation method thereof provided by the present invention are:

[0026] (1) The bacterial communities such as Bacillus subtilis and Lactobacillus plantarum in the composite microbial agent of the present invention have clear division of labor, forming an anti-disease barrier by inhibiting pathogenic bacteria, repairing intestinal mucosa, and enhancing immune activity, while promoting nutrient conversion; the anti-disease enhancing additives and the bacterial communities synergistically activate immunity, and all are natural ingredients, avoiding the risk of residual. The basic raw materials are optimized and utilize by-products, which are nutritionally balanced and low-cost, taking into account both disease resistance and nutrient absorption.

[0027] (2) The step-by-step pretreatment and aerobic-anaerobic fermentation process of the present invention are adapted to the characteristics of the bacterial flora, ensuring that the viable bacterial count meets the standard and the function is stable. The process parameters are clear and suitable for large-scale production. In application, it can improve the disease resistance of broiler chickens, reduce the incidence of disease, and reduce breeding costs. It also meets the demand for antibiotic-free breeding and is suitable for various farms.

[0028] The technical solution of the present invention is further described in detail below through examples. DETAILED DESCRIPTION

[0029] The technical solution of the present invention is further illustrated by the following examples.

[0030] In order to make the purpose, technical solutions and advantages of the present application more clear, thorough and complete, the technical solutions of the present invention are clearly and completely described below through examples. The following detailed descriptions are all descriptions of the embodiments and are intended to provide further details of the present invention. Unless otherwise specified, all technical terms used in the present invention have the same meanings as those generally understood by those skilled in the art to which this application belongs. The instruments, equipment, reagents and materials used in the examples are all obtained through commercial channels; the method steps not described in detail in the examples are all conventional technical means in the art.

[0031] The strains used in the present invention are all commercially available, specifically:

[0032] Lactobacillus acidophilus CICC 6089;

[0033] Lactobacillus plantarum subsp. plantarum ATCC 14917;

[0034] Bacillus subtilis subsp. subtilis CICC 208223;

[0035] Candida utilis CICC 1314;

[0036] Bifidobacterium bifidum CICC 6166;

[0037] Rhodopseudomonas palustris CICC 23812.

[0038] Example 1

[0039] 1.1 Microbial fermentation feed formula (by weight):

[0040] Basic ingredients: fermented soybean meal 18 parts, corn 22 parts, bran 9 parts, corn stalk powder 12 parts, rice bran 6 parts, brewer's grains 7 parts;

[0041] Composite bacterial agent: 2.2 parts of Bacillus subtilis, 1.3 parts of Lactobacillus plantarum, 2.5 parts of Lactobacillus acidophilus, 0.6 parts of Bifidobacterium bifidum, 0.6 parts of Candida utilis, 0.3 parts of Rhodopseudomonas palustris (total viable count 1.0×10 9 CFU / g, of which the number of live bacteria of Bacillus subtilis and Lactobacillus plantarum accounted for 52%, and the number of live bacteria of Rhodopseudomonas palustris accounted for 3.0×10 7 CFU / g);

[0042] Disease-resistant strengthening additives: 0.6 parts of astragalus polysaccharide, 1.2 parts of eucommia leaf extract, 0.22 parts of selenium yeast, 0.12 parts of vitamin E, and 0.3 parts of xylo-oligosaccharide.

[0043] 1.2 Preparation method:

[0044] Corn straw powder pretreatment: Take 12 parts of corn straw powder, add water at a weight ratio of 1:1.5, add 0.4% of the weight of the corn straw powder complex enzyme (cellulase: xylanase = 1:1), and enzymatic hydrolysis at 50-55°C for 2-3 hours; then add 1.5% of the weight of the corn straw powder Trichoderma reesei, and aerobically ferment for 8 hours at 30°C and a ventilation volume of 1.5 L / (L·min) to obtain pretreated straw powder.

[0045] Soybean meal-brewery grain pretreatment: 18 parts of fermented soybean meal and 7 parts of brewer's grain were mixed, 3.5% by weight of the mixture was added with water, 1.5% by weight of the mixture was inoculated with Bacillus subtilis and 0.5% by weight of the mixture was inoculated with Candida utilis, and aerobic fermentation was carried out at 30°C and an aeration rate of 1.5 L / (L·min) for 8 h to obtain a pretreated soybean meal-brewery grain mixture.

[0046] Aerobic stage: pretreated straw powder, pretreated soybean meal-brewery grains mixture, 22 parts of puffed corn, 9 parts of bran, and 6 parts of rice bran were mixed, and 1.0% of the weight of the mixed raw materials was added with Bacillus subtilis, 0.8% of the weight of the mixed raw materials, and 0.7% of the weight of the mixed raw materials was added with Rhodopseudomonas palustris. The moisture content of the material was controlled at 42-45%, and aerobic fermentation was carried out for 12 hours at 30-32° C. and an aeration rate of 1.5 L / (L·min).

[0047] Anaerobic stage: close the ventilation device, add 0.8% of the weight of aerobic fermentation product of Lactobacillus plantarum, 1.0% of the weight of aerobic fermentation product of Lactobacillus acidophilus, and 0.4% of the weight of aerobic fermentation product of Bifidobacterium bifidum, seal and ferment at 37°C for 24 hours, and control the pH of the material at 4.6-4.8 during the fermentation process.

[0048] Synergistic standing: Cool the anaerobic fermentation product to 30°C, add 0.6 parts of astragalus polysaccharide, 1.2 parts of eucommia leaf extract, 0.22 parts of selenium yeast, 0.12 parts of vitamin E, and 0.3 parts of xylo-oligosaccharide, and stand and ferment at 30°C under sealed conditions for 3-4 hours.

[0049] Finished product processing: the static fermentation product is vacuum freeze-dried at -40 to -35°C to a moisture content of ≤7%, crushed and passed through a 40-mesh sieve to obtain microbial fermentation feed.

[0050] Example 2

[0051] The difference from Example 1 is that the formula dosage of the microbial fermentation feed is (by weight):

[0052] Basic ingredients: fermented soybean meal 22 parts, corn 28 parts, bran 11 parts, corn stalk powder 14 parts, rice bran 7 parts, brewer's grains 9 parts;

[0053] Composite bacterial agent: 2.8 parts of Bacillus subtilis, 1.7 parts of Lactobacillus plantarum, 3.5 parts of Lactobacillus acidophilus, 0.8 parts of Bifidobacterium bifidum, 0.9 parts of Candida utilis, 0.4 parts of Rhodopseudomonas palustris (total viable count 1.5×10 9 CFU / g, of which the number of live bacteria of Bacillus subtilis and Lactobacillus plantarum accounted for 55%, and the number of live bacteria of Rhodopseudomonas palustris accounted for 5.0×10 7 CFU / g);

[0054] Disease-resistant strengthening additives: 0.9 parts of astragalus polysaccharide, 1.8 parts of eucommia leaf extract, 0.28 parts of selenium yeast, 0.18 parts of vitamin E, and 0.5 parts of xylo-oligosaccharide.

[0055] Other conditions are the same as in Example 1.

[0056] Example 3

[0057] The difference from Example 1 is that the formula dosage of the microbial fermentation feed is (by weight):

[0058] Basic raw materials: fermented soybean meal 20 parts, corn 25 parts, bran 10 parts, corn straw powder 13 parts, rice bran 6.5 parts, brewer's grains 8 parts;

[0059] Composite bacterial agent: 2.5 parts of Bacillus subtilis, 1.5 parts of Lactobacillus plantarum, 3.0 parts of Lactobacillus acidophilus, 0.7 parts of Bifidobacterium bifidum, 0.75 parts of Candida utilis, 0.35 parts of Rhodopseudomonas palustris (total viable count 1.2×10 9 CFU / g, of which the number of live bacteria of Bacillus subtilis and Lactobacillus plantarum accounted for 53%, and the number of live bacteria of Rhodopseudomonas palustris accounted for 4.0×10 7 CFU / g);

[0060] Disease-resistant strengthening additives: 0.75 parts of astragalus polysaccharide, 1.5 parts of eucommia leaf extract, 0.25 parts of selenium yeast, 0.15 parts of vitamin E, and 0.4 parts of xylo-oligosaccharide.

[0061] Other conditions are the same as in Example 1.

[0062] Comparative Example 1

[0063] The difference from Example 3 is that the astragalus polysaccharide in the disease-resistant strengthening additive is replaced by lentinan, and the dosage of other ingredients and the preparation process are the same as in Example 3.

[0064] Comparative Example 2

[0065] The difference from Example 3 is that the selenium yeast in the disease-resistant strengthening additive is replaced by sodium selenite, and the amounts of other raw materials and the preparation process are the same as in Example 3.

[0066] Comparative Example 3

[0067] The difference from Example 3 is that vitamin E in the disease-resistant strengthening additive is replaced by vitamin C, and the amounts of other raw materials and the preparation process are the same as in Example 3.

[0068] Comparative Example 4

[0069] The difference from Example 3 is that the xylooligosaccharide in the disease-resistant strengthening additive is replaced by fructooligosaccharide, and the amounts of other raw materials and the preparation process are the same as in Example 3.

[0070] Comparative Example 5

[0071] The difference from Example 3 is that the Eucommia leaf extract in the disease-resistant strengthening additive is replaced by honeysuckle extract, and the other raw material amounts and preparation process are the same as in Example 3.

[0072] Effect verification:

[0073] Experimental animals and groups:

[0074] 450 one-day-old healthy white-feathered broiler chickens (initial body weight 42±2 g) were selected and randomly divided into 9 groups, with 5 replicates in each group and 10 chickens in each replicate, namely: Example 1 Group, Example 2 Group, Example 3 Group, and Comparative Examples 1-5 Groups (1 group each).

[0075] Feeding and management:

[0076] The experimental period was 42 days. The feeding environment was consistent (temperature 25-28°C, humidity 60-65%, natural light). The rats had free access to food and water. The feed intake, morbidity and mortality were recorded daily.

[0077] Testing indicators and methods:

[0078] 1. Growth Performance Measurement: The broilers were weighed at 42 days of age, and the average daily weight gain (total weight gain / 42 days) and feed-to-meat ratio (total feed intake / total weight gain) of the broilers during the experimental period were calculated. The results are shown in Table 1.

[0079] Table 1 Average daily weight gain and feed-to-meat ratio of broiler chickens

[0080] Average daily weight gain (g) Feed-to-meat ratio Example 1 59.8±1.2 1.88±0.03 Example 2 62.3±1.5 1.81±0.02 Example 3 64.5±1.3 1.76±0.02 Comparative Example 1 54.2±1.1 2.05±0.04 Comparative Example 2 55.1±1.4 2.02±0.03 Comparative Example 3 53.6±1.2 2.08±0.03 Comparative Example 4 52.8±1.3 2.12±0.04 Comparative Example 5 54.7±1.5 2.03±0.03

[0081] The average daily weight gain of Examples 1-3 (59.8-64.5 g) was significantly higher than that of Comparative Examples 1-5 (54.2-55.1 g) (P<0.05), indicating that the feed of the present application can continuously promote the growth of broiler chickens.

[0082] The feed-to-meat ratio of the example group (1.76-1.88) was significantly lower than that of the control group (2.02-2.12) (P<0.05), demonstrating that the composite bacterial agent had a synergistic effect in degrading nutrients and the anti-disease additive in enhancing absorption: oligoxylose promoted bacterial proliferation and increased dietary fiber utilization, while astragalus polysaccharide enhanced intestinal absorption function, thereby jointly reducing the feed-to-meat ratio.

[0083] 2. Morbidity test: The number of animals with diarrhea, enteritis and other intestinal diseases in each group was recorded daily, and the incidence rate was calculated as (total number of animals with the disease / total number of animals) × 100%. The results are shown in Table 2.

[0084] Table 2 Incidence of disease in broiler chickens

[0085]

[0086]

[0087] The incidence of intestinal diseases in Example 1-3 (2.5%-4.2%) was significantly lower than that in Comparative Example 1-5 (8.5%-11.5%) (P<0.05). In addition to the antimicrobial peptides produced by Bacillus subtilis inhibiting Escherichia coli and the Eucommia leaf extract activating T cells to enhance anti-infection ability, it also benefited from the synergistic effect of multiple components: Lactobacillus acidophilus and Lactobacillus plantarum in the composite bacterial agent metabolize and produce acid (intestinal pH drops to 4.6-4.8), which can inhibit the proliferation of acid-intolerant pathogens; Bifidobacterium bifidum adheres to the intestinal mucosa to form a biological barrier, reducing the colonization of pathogens. The oligofructose in the anti-disease additive specifically promotes the proliferation of beneficial bacteria and strengthens the competitive advantage of the bacterial flora; selenium yeast and vitamin E synergistically resist oxidation, reduce oxidative damage to the intestinal mucosa, and maintain barrier integrity. This multi-dimensional synergy jointly reduces the risk of intestinal diseases.

[0088] 3. Immune function testing:

[0089] Serum immunoglobulins: Wing vein blood was collected and centrifuged to prepare serum. Serum IgG and IgM levels were measured using ELISA (kit purchased from Shanghai ELISA Biotechnology Co., Ltd.) according to the manufacturer's instructions. The results are shown in Table 3.

[0090] Table 3 Serum immunoglobulin levels in broiler chickens

[0091]

[0092]

[0093] The serum IgG and IgM levels in Example 1-3 were significantly higher than those in Comparative Examples 1-5. The key is the synergistic activation of multiple components: Astragalus polysaccharide directly targets and stimulates the proliferation of B cells and plasma cells, driving antibody synthesis; Eucommia leaf extract amplifies immune signals by enhancing the antigen presentation efficiency of dendritic cells, and the two form a humoral immune closed loop. At the same time, the short-chain fatty acids (such as propionic acid) produced by the metabolism of Lactobacillus acidophilus and Candida utilis in the composite microbial agent can improve the intestinal immune microenvironment, promote the differentiation of mucosal B cells into plasma cells, and indirectly increase serum antibody levels; vitamin E protects immune cells from free radical damage through antioxidants, maintaining their ability to continuously secrete antibodies.

[0094] In comparative example 1, lentinan lacks the specific activation of B cells by astragalus polysaccharide, honeysuckle extract is difficult to enhance antigen presentation, and the breakage of the synergistic chain leads to insufficient antibody production; in comparative example 2, after sodium selenite is used to replace selenium yeast, the insufficient supply of organic selenium reduces the synthesis of glutathione peroxidase, and the activity of immune cells decreases due to oxidative damage, further inhibiting the antibody secretion function of plasma cells.

[0095] Therefore, the present invention adopts the above-mentioned microbial fermented feed for improving the disease resistance of broilers and its preparation method. The prepared microbial fermented feed significantly improves the nutrient utilization and the intestinal barrier function of broilers through the synergistic mechanism of composite bacterial agents and disease-resistant strengthening additives, reduces the occurrence of intestinal diseases, and is suitable for the disease-resistant feed needs in large-scale broiler farming.

[0096] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit the same. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solutions of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A microbial fermented feed for improving disease resistance of broiler chickens, characterized in that: include: Basic raw materials: fermented soybean meal, corn, bran, corn straw powder, rice bran, brewer's grains; Composite bacterial agent: Lactobacillus acidophilus, Lactobacillus plantarum, Bacillus subtilis, Candida utilis, Bifidobacterium bifidum, Rhodopseudomonas palustris; Disease-resistant strengthening additives: selenium yeast, eucommia leaf extract, astragalus polysaccharide, oligoxylose, vitamin E.

2. The microbial fermented feed according to claim 1, characterized in that The amount of each component is calculated by weight: 18-22 parts of fermented soybean meal, 12-14 parts of corn straw powder, 22-28 parts of corn, 9-11 parts of bran, 6-7 parts of rice bran, 7-9 parts of brewer's grains, 2.2-2.8 parts of Bacillus subtilis, 1.3-1.7 parts of Lactobacillus plantarum, 2.5-3.5 parts of Lactobacillus acidophilus, 0.6-0.8 parts of Bifidobacterium bifidum, 0.6-0.9 parts of Candida utilis, 0.3-0.4 parts of Rhodopseudomonas palustris, 1.2-1.8 parts of eucommia leaf extract, 0.6-0.9 parts of astragalus polysaccharide, 0.22-0.28 parts of selenium yeast, 0.12-0.18 parts of vitamin E, and 0.3-0.5 parts of xylo-oligosaccharide.

3. The microbial fermented feed according to claim 1, characterized in that The total number of viable bacteria in the composite bacterial agent is ≥1×10 9 CFU / g, of which the number of live bacteria of Bacillus subtilis and Lactobacillus plantarum accounts for ≥50%, and the number of live bacteria of Rhodopseudomonas palustris ≥3×10 7 CFU / g.

4. The method for preparing a microbial fermented feed for improving disease resistance of broiler chickens according to any one of claims 1 to 3, characterized in that: The following steps are involved: S1. Corn straw powder pretreatment: corn straw powder was mixed with water, enzymatically hydrolyzed with a complex enzyme, and then aerobic fermentation with Trichoderma reesei was carried out; S2, soybean meal-brewery grain pretreatment: fermented soybean meal and brewer's grain are mixed, water is added, and then inoculated with Bacillus subtilis and Candida utilis for aerobic fermentation; S3, aerobic stage: all pretreated raw materials are mixed with puffed corn, bran, and rice bran, and inoculated with Bacillus subtilis, Candida utilis, and Rhodopseudomonas palustris for aerobic fermentation; S4, anaerobic stage: ventilation is turned off, and Lactobacillus plantarum, Lactobacillus acidophilus, and Bifidobacterium bifidum are introduced for sealed fermentation; S5, cooling the anaerobic fermentation product, adding disease-resistant strengthening additives, and allowing it to ferment; S6. The fermentation product is vacuum freeze-dried and crushed to obtain microbial fermentation feed.

5. The preparation method according to claim 4, characterized in that In step S1, corn straw powder and water are mixed in a weight ratio of 1:1.5; the complex enzyme is composed of cellulase and xylanase in a weight ratio of 1:1, and the added amount is 0.4% of the weight of the corn straw powder; the enzymatic hydrolysis temperature is 50-55°C, and the enzymatic hydrolysis time is 2-3 hours; the inoculum amount of Trichoderma reesei is 1.5% of the weight of the corn straw powder, the aerobic fermentation temperature is 30°C, the ventilation volume is 1.5 L / (L·min), and the fermentation time is 8 hours.

6. The preparation method according to claim 4, characterized in that In step S2, fermented soybean meal and brewer's grains are mixed in a weight ratio of 2:1; the amount of water added is 3.5% of the weight of the mixture; the amount of Bacillus subtilis inoculated is 1.5% of the weight of the mixture, and the amount of Candida utilis inoculated is 0.5% of the weight of the mixture; the aerobic fermentation temperature is 30° C., the ventilation volume is 1.5 L / (L·min), and the fermentation time is 8 hours.

7. The preparation method according to claim 4, characterized in that In step S3, the inoculum amount of Bacillus subtilis is 1.0% by weight of the mixed raw materials, the inoculum amount of Candida utilis is 0.8% by weight of the mixed raw materials, and the inoculum amount of Rhodopseudomonas palustris is 0.7% by weight of the mixed raw materials; the moisture content of the material is controlled at 42-45%; the aerobic fermentation temperature is 30° C., the ventilation volume is 1.5 L / (L·min), and the fermentation time is 12 h.

8. The preparation method according to claim 4, characterized in that In step S4, the inoculation amount of Lactobacillus plantarum is 0.8% of the weight of the aerobic fermentation product, the inoculation amount of Lactobacillus acidophilus is 1.0% of the weight of the aerobic fermentation product, and the inoculation amount of Bifidobacterium bifidum is 0.4% of the weight of the aerobic fermentation product; the anaerobic fermentation temperature is 37° C., the fermentation time is 24 hours, and the pH of the material is controlled at 4.6-4.8 during the fermentation process.

9. The preparation method according to claim 4, characterized in that In step S5: after the anaerobic fermentation product is cooled to 30° C., an anti-disease strengthening additive is added; the fermentation temperature is kept at 30° C. for 3-4 hours, and the container is kept sealed during the fermentation process.

10. The preparation method according to claim 4, characterized in that In step S6: the freeze-drying temperature is -40 to -35°C, and the moisture content of the material after drying is ≤7%; and the material is sieved through a 40-mesh sieve after being crushed.

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