Composition containing cactus fermentation liquor as well as preparation method and application of composition in cosmetics

Abstract

The invention discloses a composition containing cactus fermentation liquor and a preparation method and application of the composition in cosmetics, the cosmetic composition containing the cactus fermentation liquor comprises the cactus fermentation liquor, Ectoine and sodium hyaluronate according to a specific proportion, the cactus fermentation liquor is prepared by performing enzymolysis pretreatment on succulent stems of cactus, and the cactus fermentation liquor is prepared by performing enzymolysis pretreatment on the succulent stems of the cactus. Sequentially inoculating bacillus licheniformis and lactobacillus plantarum for sequential fermentation; and finally, purifying and enriching by ultrafiltration and nanofiltration two-stage membrane separation technologies. A product rich in small molecule active peptide and specific organic acid is obtained through biotransformation process combination, so that the final cosmetic composition has remarkable skin barrier repairing and anti-oxidation effects.

Description

Technical Field

[0001] The present invention relates to the fields of cosmetic technology and biological fermentation technology, and in particular to a composition containing cactus fermentation liquid, a preparation method thereof, and application in cosmetics. Background Art

[0002] In the field of modern cosmetics, seeking active ingredients that are derived from nature, safe and effective has become a mainstream trend, among which the use of plant ingredients has attracted particular attention.

[0003] As a tenacious plant, cactus has been widely studied in the industry because its fleshy stems are rich in mucopolysaccharides, flavonoids, vitamins and various amino acids. It is believed to have excellent moisturizing, soothing and antioxidant potential in cosmetics. However, in order to efficiently convert these potential benefits into perceptible skin improvement effects for consumers, existing technical means still face significant bottlenecks.

[0004] While traditional solvent extraction methods (such as water or alcohol) can yield cactus extracts, they struggle to effectively penetrate the robust cell wall barrier of the cactus plant, resulting in insufficient dissolution of the active essence within. Furthermore, the extracts are primarily composed of large-molecule polysaccharides, significantly limiting their permeability and bioavailability on the skin surface, hindering their potential for deep skincare. The industry has introduced biofermentation technology to further process plant raw materials, hoping to degrade these macromolecules into functional small molecules that are readily absorbed by the skin through microbial metabolism. However, conventional fermentation processes currently used in the development of cosmetic raw materials typically utilize a single bacterial species (such as yeast or lactic acid bacteria), which is insufficient for processing complex plant matrices such as cactus, which are rich in cellulose and pectin. The limited enzyme repertoire and metabolic pathways of a single bacterial species limit its cell wall-breaking ability and substrate conversion efficiency, ultimately leading to incomplete fermentation and the production of a limited variety of effective metabolites (such as specific organic acids and functional oligopeptides) with low yields. Especially as consumers increasingly demand more effective skincare products, the market urgently needs innovative solutions that can effectively repair the skin barrier and combat skin issues caused by modern environmental stressors like PM2.5. However, cactus ingredients derived from traditional extraction or conventional fermentation techniques are clearly insufficient to meet these high-end skincare needs. Therefore, the challenge of breaking through conventional methods and developing an innovative process for efficient and deep biotransformation of cactus remains a pressing technical challenge. Summary of the Invention

[0005] The purpose of the present invention is to solve the defects in the prior art and to propose a composition containing cactus fermentation liquid, a preparation method and application in cosmetics.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions: A cosmetic composition containing cactus fermentation liquid, comprising the following components by weight: 0.1-25% of cactus fermentation liquid, 0.1-2% of ectoine, and 0.02-0.5% of sodium hyaluronate; The cactus fermentation liquid is prepared by a method comprising sequential fermentation of cactus fleshy stems, wherein the sequential fermentation comprises: inoculating a first microorganism in an initial system for a first stage of anaerobic fermentation, and then inoculating a second microorganism for a second stage of facultative anaerobic fermentation.

[0007] Furthermore, before inoculating the first microorganism, the method further includes a step of enzymatically pre-treating the initial system with cellulase and pectinase. In a preferred embodiment, the enzymatic pre-treatment comprises hydrolysis at a pH of 4.5-5.0 and a temperature of 48-52°C for 2-3 hours, wherein the ratio of cellulase to pectinase activity units in the complex enzyme preparation is 2-3:1.

[0008] Furthermore, the first microorganism is Bacillus licheniformis, and the second microorganism is Lactobacillus plantarum; The first preset conditions include: temperature of 35-38°C, pH of 6.0-6.5, and time of 20-28 hours; The second preset conditions include: temperature of 26-30° C., pH value of 4.5-5.5, and time of 40-50 hours.

[0009] Furthermore, the cactus fermentation broth obtained by the above process has a unique chemical fingerprint. In a preferred embodiment, the weight ratio of lactic acid to acetic acid is 5:1 to 8:1, and the content of oligopeptides with a molecular weight of less than 1000 Daltons accounts for more than 70% of the total polypeptide content.

[0010] Furthermore, the preparation method further comprises a post-processing step after the fermentation is completed, wherein the step preferably comprises removing impurities using an ultrafiltration membrane with a molecular weight cutoff of 10 kDa, and then concentrating using a nanofiltration membrane with a molecular weight cutoff of 500 Da.

[0011] Another object of the present invention is to provide a method for preparing the cosmetic composition, which is prepared by mixing the cactus fermentation liquid, ectoine, sodium hyaluronate, and auxiliary materials, wherein the auxiliary materials include at least one of a moisturizer, an emollient, a thickener, and a preservative.

[0012] Preferably, the method comprises heating the aqueous phase component and the oil phase component separately, homogenizing and emulsifying them, and then adding the cactus fermentation liquid after cooling.

[0013] Another object of the present invention is to provide an application of the cosmetic composition, specifically its application in preparing cosmetics for repairing skin barrier function, and in preparing cosmetics for long-lasting moisturizing and improving skin gloss.

[0014] Compared with the prior art, the present invention has the following beneficial effects: Through the synergistic action of cellulase and pectinase, the strong cell wall physical barrier of the cactus raw material is effectively broken, the initial release rate of its internal active ingredients is greatly improved, and a richer nutrient substrate is provided for subsequent deep fermentation. On this basis, the present invention further adopts a dual-strain sequential fermentation process, utilizing the powerful macromolecular degradation ability of Bacillus licheniformis and the efficient acid production and conversion of small molecules by Lactobacillus plantarum, forming a "relay-type", deep and directional biotransformation process. The unique chemical fingerprint with a specific acid-base ratio and a high content of small molecule oligopeptides produced by this process is unattainable by conventional single-strain fermentation. Finally, through secondary membrane separation and purification technology, the fermentation product is precisely cut and enriched by molecular weight, which can effectively remove invalid macromolecular impurities and significantly increase the concentration of functional small molecules, thereby greatly enhancing the skin permeability and bioavailability of the final product, so that the cosmetic composition containing the cactus fermentation liquid can provide deeper nourishment to the skin, thereby enhancing the skin barrier repair ability and the potential to resist environmental stress. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention.

[0016] Figure 1 Schematic diagram of the process flow of the method for preparing cactus fermentation broth in an embodiment of the present invention; Figure 2a A comparative bar graph showing semi-quantitative scores of oligopeptide content in the products obtained in the examples of the present invention and in a plurality of comparative examples; Figure 2b A comparative bar chart showing the semi-quantitative scores of the antioxidant activities of the products obtained in the examples of the present invention and in several comparative examples. Figure 3a A bar graph showing the efficacy scores of the cosmetic compositions according to the embodiments of the present invention in self-assessment by consumers; Figure 3b This is a bar graph showing the efficacy scores of the composition of Comparative Example 1 in the embodiments of the present invention in consumers' self-assessment. DETAILED DESCRIPTION

[0017] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0018] In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as limiting the present invention.

[0019] Preparation Example 1 like Figure 1 As shown, the embodiment of the present invention provides a method for preparing a composition containing cactus fermentation liquid, and the specific preparation process is as follows: Step 1: Weigh 100 g of fresh stem flesh of Opuntia milpai cactus that has been cleaned and spineless, add 600 g of purified water, and beat at high speed in a tissue blender for 2 minutes to prepare a uniform initial slurry; The pH of the slurry was adjusted to 4.8 using citric acid. 0.8 g of a complex enzyme preparation (with a cellulase to pectinase activity ratio of 2.5:1) was added to the slurry and hydrolyzed at 60 rpm in a 50°C constant temperature magnetic stirring water bath for 2.5 hours. After the hydrolysis is completed, the enzymatic solution is sterilized by high-pressure steam at 121°C for 20 minutes and naturally cooled to room temperature for use; Step 2: The pH value of the cooled sterile enzymatic hydrolysate was adjusted to 6.2, and then an activated culture of a strain of Bacillus licheniformis was aseptically inoculated at a 2% (v / v) inoculum volume, and cultured at 37°C under anaerobic conditions (sterile nitrogen was filled at the top of the fermenter to expel air) for 24 hours to obtain a primary fermentation product; The activated culture broth of a strain of Lactobacillus plantarum was aseptically inoculated into the primary fermentation product at a 3% (v / v) inoculum amount. The pH of the fermentation broth was adjusted to 5.0. The fermentation was continued at 28°C under facultative anaerobic conditions for 45 hours to obtain a final fermentation broth.

[0020] Step 3: The final fermentation broth is first filtered through an ultrafiltration membrane with a molecular weight cutoff of 10 kDa to remove bacteria and macromolecular impurities, and the clear permeate is collected. Subsequently, the permeate is filtered through a nanofiltration membrane with a molecular weight cutoff of 500 Da for nanofiltration concentration. When the feed liquid is concentrated to 1 / 10 of its original volume, the concentrated liquid is collected; The concentrate was sterilized by filtering through a 0.22 μm filter membrane to obtain the final cactus fermentation liquid, which was sealed and stored at 4°C.

[0021] Example 1: This embodiment provides an intensive repair essence, the components of which include, by weight percentage: 20.00% of the cactus fermentation liquid prepared in Preparation Example 1, 1.00% of ectoine, 0.30% of sodium hyaluronate of combined molecular weight, 0.20% of bisabolol, 5.00% of 1,3-butanediol, 3.00% of glycerin, 0.15% of xanthan gum, 0.80% of a mixture of phenoxyethanol and ethylhexylglycerin as preservatives, and the balance being purified water.

[0022] The preparation method is as follows: Add the formulated amount of purified water to a preparation tank. While stirring, add glycerin, 1,3-butylene glycol, sodium hyaluronate, and a preservative in sequence, stirring until completely dissolved and mixed. Slowly disperse xanthan gum as a thickener while stirring, continuing to stir until it is fully hydrated and forms a uniform, transparent gel matrix. Cool the system to below 40°C, then add the formulated amount of cactus fermentation broth, ectoine, and bisabolol in sequence. Continue stirring for 20 minutes to ensure uniform mixing of all components, resulting in a clear serum product.

[0023] Example 2: This example provides a barrier-repairing, moisturizing facial cream, comprising, by weight, a phase A (aqueous phase) and a phase B (oil phase). Phase A comprises: 8.00% of the cactus fermentation broth prepared in Preparation Example 1, 1.50% ectoine, 0.10% sodium hyaluronate, 5.00% glycerin, 1.00% panthenol (vitamin B5), 0.70% phenoxyethanol as a preservative, and the balance purified water. Phase B comprises: 6.00% squalane and 4.00% shea butter as emollients, 0.50% ceramide NP as a barrier-repairing ingredient, 3.00% cetearyl alcohol as an emulsion stabilizer, and 2.00% glyceryl stearate SE as an emulsifier.

[0024] The specific preparation method is as follows: all components of phase A except the cactus fermentation liquid are mixed in a main pot, heated to 75-80°C, stirred to completely dissolve, and kept warm for later use. At the same time, all components of phase B are mixed in an oil phase pot, heated to 75-80°C, and stirred to completely melt into a uniform oil phase. Turn on the homogenization stirring of the main pot (5000rpm), and slowly pump the oil phase into the main pot for homogenization and emulsification with the water phase for 3 minutes to form a uniform and delicate O / W emulsion system. Turn off the homogenization, start slow stirring, and slowly cool the emulsification system. When the system temperature cools to below 45°C, add the formulated amount of cactus fermentation liquid and continue stirring until the material is completely uniform, finally obtaining a facial cream product with a rich texture.

[0025] Example 3: The present embodiment provides an essence for a sheet mask, the components of which include, by weight percentage: 12.00% of the cactus fermentation liquid prepared in Preparation Example 1, 0.50% of ectoine, 0.40% of sodium hyaluronate, 2.00% of purslane extract as a soothing ingredient, 8.00% of 1,3-butylene glycol as a moisturizer, 0.20% of carbomer 940 as a thickener, 0.18% of triethanolamine as a pH regulator, 0.20% of methyl parahydroxybenzoate as a preservative, and the balance being purified water. Its preparation method is similar to that of Example 1, and both are prepared using an aqueous phase system.

[0026] In order to better understand the technical solution of the present invention, the following is further explained through experiments.

[0027] The following four comparative examples were prepared. The basic formulas of all comparative examples (including all other components except the cactus product and their contents, dosage forms and preparation processes) were exactly the same as those in Example 2, and the only difference was the core raw materials.

[0028] Comparative Example 1 (blank control): This comparative example differs from Example 2 only in that the formula does not contain any cactus product, and 8.00% of the cactus fermentation liquid in the original formula is replaced by an equal amount of purified water.

[0029] Comparative Example 2 (unfermented control): The only difference between this comparative example and Example 2 is that its core raw material is replaced by a cactus water extract that has not undergone any fermentation and enzymatic hydrolysis (i.e., the liquid obtained by directly filtering after pulping in step 1 of Preparation Example 1).

[0030] Comparative Example 3 (conventional fermentation control): This comparative example differs from Example 2 only in that its core raw material is replaced by a product obtained by fermentation using a conventional single Lactobacillus plantarum strain (i.e., the product obtained by skipping the enzymatic hydrolysis and Bacillus licheniformis fermentation steps of Preparation Example 1).

[0031] Comparative Example 4 (no special process control): The only difference between this comparative example and Example 2 is that its core raw material is replaced by a product obtained by sequential fermentation but omitting the key enzymatic pretreatment step and secondary membrane separation and purification step.

[0032] Experimental example: The sample used in the experiment was the barrier repair special moisturizing cream prepared in Example 2, and the comparative samples used were Comparative Examples 1-4.

[0033] Experimental Example 1: Comparison of acidity changes: Testing Procedure: Add 100 mL of the fermentation broth prepared by the process of Preparation Example 1 (the product of the present invention) and 100 mL of the fermentation broth prepared by the process of Comparative Example 3 (the conventional fermentation product) to two identical clean beakers. Use a calibrated portable pH meter to measure the initial pH of each liquid and photograph the readings. Subsequently, place the two beakers in the same environment, and measure and record the pH every 24 hours for 72 hours.

[0034] The comparison of pH changes in different fermentation processes is shown in Table 1 Table 1 Time point Product of the present invention (pH value) Conventional fermentation products (pH value) 0h 6.2 6.2 24h 5.1 5.8 48h 4.3 5.3 72h 3.8 4.9 From the above, it can be seen that the pH value decrease rate and the final acidity achieved of the product of the present invention are better than those of conventional fermentation products, which shows that the sequential fermentation process of the present invention has a higher acid production efficiency.

[0035] Ninhydrin color reaction: Testing Procedure: The product of the present invention and samples from Comparative Examples 2-4 were reacted with ninhydrin and then semi-quantitatively scored. Scoring Criteria: Color depth is categorized into 5 levels (0-4): 0 = colorless; 1 = light purple; 2 = medium purple; 3 = dark purple; 4 = very dark bluish purple. Three independent blind reviewers scored each tube, and the average score was used as the final result, as shown in Figures 2A and 2B. The color depth scores for the ninhydrin reaction are shown in Table 2.

[0036] Table 2 sample Score mean ± standard deviation Product of the present invention 3.7±0.3 Comparative Example 2 0.3±0.1 Comparative Example 3 1.3±0.2 Comparative Example 4 2.0±0.3 As can be seen from the above, the reaction tube of the product of the present invention exhibits the deepest blue-purple color, and the color depth far exceeds that of all the comparative examples, proving that it has the strongest ability to efficiently decompose proteins into free amino acids and oligopeptides.

[0037] Experimental Example 2: Testing Procedure: The samples from Example 2 and Comparative Examples 1-4 were reacted with a DPPH solution and then semi-quantitatively scored. Scoring Criteria: The degree of fading of the DPPH solution was categorized into 5 levels (0-4): 0 = no fading (purple); 1 = slight fading; 2 = moderate fading; 3 = significant fading; 4 = almost complete fading (colorless / light yellow). Three independent blind reviewers also scored the samples, and the average score was taken. This is shown in Figures 3A and 3B. The DPPH free radical scavenging ability score (maximum 4 points) is shown in Table 3.

[0038] Table 3 sample Score mean ± standard deviation Product of the present invention 3.3±0.2 Comparative Example 1 0.0±0.0 Comparative Example 2 0.7±0.1 Comparative Example 3 1.0±0.3 Comparative Example 4 1.7±0.2 From the above, it can be seen that the composition of the present invention has strong antioxidant activity, and its efficacy score is significantly higher than that of all comparative examples.

[0039] Experimental Example 3: Test steps: 10 volunteers were recruited for a 7-day "half-face" test. Each morning and evening, the volunteers were asked to use the cream prepared according to Example 2 on their left cheeks and the cream prepared in Comparative Example 1 (blank control) on their right cheeks. After the test period, volunteers anonymously filled out a standardized "Skin Self-Assessment Questionnaire" based on their true feelings on the left and right faces, and scored the three dimensions of "skin moisturization", "smoothness", and "improvement of dryness and tightness" on a scale of 1-5 (1 = no improvement, 5 = great improvement).

[0040] The average self-assessment scores (maximum 5 points) of skin improvement after 7 days of use are shown in Table 4.

[0041] Table 4 Evaluation Dimensions Example 2 (left face) Comparative Example 1 (right face) Skin moisturization 4.6±0.5 2.1±0.4 Skin smoothness 4.3±0.6 2.3±0.5 Improvement of dryness and tightness 4.5±0.4 1.9±0.3 As can be seen from the above, the average scores of the half face using Example 2 in all evaluation dimensions are significantly higher than those of the half face using Comparative Example 1, thereby proving that the solution of the present invention has excellent moisturizing and repairing effects, and has real, perceptible, and statistically significant excellent effects in moisturizing and improving skin feel.

[0042] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A method for preparing cactus fermentation liquid, characterized in that: The following steps are involved: S1: adding a complex enzyme preparation of cellulase and pectinase to an initial system containing cactus fleshy stems, and performing hydrolysis at a pH of 4.5-5.0 and a temperature of 48-52°C to break the physical outer barrier of the cactus cell wall; S2: first inoculating Bacillus licheniformis into the system treated in step S1 to perform a first stage anaerobic fermentation under first preset conditions, and then inoculating Lactobacillus plantarum to perform a second stage facultative anaerobic fermentation under second preset conditions to achieve deep biotransformation of the cactus contents; S3: The fermented liquid is first removed from the fermentation liquid using an ultrafiltration membrane with a molecular weight cutoff of 10kDa, and then concentrated and enriched using a nanofiltration membrane with a molecular weight cutoff of 500Da to obtain high-purity small molecule active substances.

2. The method for preparing cactus fermented liquid according to claim 1, wherein: The first preset conditions include: temperature of 35-38°C, pH of 6.0-6.5, and time of 20-28 hours; The second preset conditions include: temperature of 26-30° C., pH value of 4.5-5.5, and time of 40-50 hours.

3. A cactus fermentation liquid prepared by the method for preparing cactus fermentation liquid according to claim 2.

4. The cactus fermented liquid according to claim 3, characterized in that The weight ratio of lactic acid to acetic acid is 5:1 to 8:

1.

5. The cactus fermented liquid according to claim 4, characterized in that The content of oligopeptides with a molecular weight of less than 1000 Daltons accounts for more than 70% of the total polypeptide content.

6. A cosmetic composition containing cactus fermentation liquid, characterized in that: The invention comprises, by weight percentage, 0.1-25% of the cactus fermentation liquid according to any one of claims 3-5, 0.1-2% of ectoine and 0.02-0.5% of sodium hyaluronate.

7. The cosmetic composition containing cactus fermentation liquid according to claim 6, characterized in that: The invention also comprises at least one auxiliary material selected from the group consisting of a moisturizer, an emollient, a thickener and a preservative.

8. A method for preparing a cosmetic composition containing cactus fermentation liquid according to claim 7, characterized in that: The method comprises the step of mixing the cactus fermentation liquid with other components.

9. Use of the cosmetic composition containing cactus fermentation liquid according to claim 7 in preparing cosmetics for repairing skin barrier.

10. Use of the cosmetic composition containing cactus fermentation liquid according to claim 7 in the preparation of cosmetics for resisting skin damage caused by environmental stress.

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