Probiotic granules capable of improving intestinal functions as well as preparation method and application of probiotic granules

Abstract

The invention belongs to the technical field of probiotic particles, and particularly relates to probiotic particles for improving intestinal functions as well as a preparation method and application of the probiotic particles. The probiotic particles capable of improving the intestinal function comprise a probiotic core, a first embedding layer, a second embedding layer and a third embedding layer, the probiotic inner core is prepared from plant lactobacillus, bifidobacterium animalis, lactobacillus acidophilus, bifidobacterium longum, lactobacillus paracasei, lactobacillus rhamnosus, lactobacillus fermentum, lactobacillus helveticus, streptococcus thermophilus, plant lactobacillus, lactobacillus helveticus and lactobacillus rhamnosus. According to the present invention, the twelve probiotics are contained, and the latest three-layer embedding technology is adopted so as to enhance the storage property and the digestive tract tolerance, such that the product can maintain the beneficial bacterium activity for a long time without refrigeration, and the product is suitable for the crowd with the alteration of the intestinal flora.

Description

Technical Field

[0001] The present invention belongs to the technical field of probiotic granules, and particularly relates to probiotic granules for improving intestinal function, and a preparation method and application thereof. Background Art

[0002] Probiotics are a class of active microorganisms that are beneficial to host health and are widely used in food, health supplements, and medicine. They exert various physiological functions by regulating the balance of the intestinal microbiome, enhancing immune function, and inhibiting the growth of pathogens. However, probiotics are susceptible to external environmental factors (such as temperature, humidity, and oxygen) during production, transportation, and storage, which can significantly reduce their activity and thus affect the product's efficacy.

[0003] Most probiotic products currently on the market require low-temperature refrigeration to maintain their activity and stability. This heavy reliance on cold chain transportation and storage not only increases production and logistics costs but also limits the product's scope of use. This is particularly true in areas lacking refrigeration or in high-temperature environments, making it difficult to ensure the effectiveness and safety of probiotics.

[0004] Furthermore, after entering the human digestive tract, probiotics must withstand the harsh conditions of stomach acid, bile salts, and other environmental challenges. Many traditional probiotic strains become inactivated during this process, making it difficult for them to reach the intestines and exert their intended effects. Therefore, improving the stability of probiotics during storage and their tolerance in the digestive tract has become a key technical challenge in the current development of probiotic products.

[0005] Chinese patent CN 109464425 B discloses a probiotic-encapsulated particle and its preparation method. The product has a viable bacterial survival rate of 39-49% after encapsulation; a survival rate of 18-42% after accelerated storage at 37°C for 2 months; and a survival rate of 77-85% after 60 minutes in artificial gastric fluid.

[0006] In order to solve the above problems, it is urgent to develop a new type of probiotic granules that can maintain high activity for a long time under non-refrigerated conditions and have good digestive tract tolerance, so as to improve the practicality, stability and functionality of the product and meet consumers' demand for high-quality probiotic products. Summary of the Invention

[0007] The purpose of the present invention is to provide a probiotic granule for improving intestinal function and a preparation method and application thereof.

[0008] In order to achieve the above object, the present invention provides the following technical solutions:

[0009] A probiotic particle for improving intestinal function comprises a probiotic core, a first embedding layer, a second embedding layer and a third embedding layer; the probiotic core comprises Lactobacillus plantarum, Bifidobacterium animalis, Lactobacillus acidophilus, Bifidobacterium longum, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus fermentum, Lactobacillus helveticus, Streptococcus thermophilus, Lactobacillus plantarum, Lactobacillus helveticus and Lactobacillus rhamnosus.

[0010] Preferably, the probiotic core includes Lactobacillus plantarum LP28, Bifidobacterium animalis BAL06, Lactobacillus acidophilus LA1063, Bifidobacterium longum BL986, Lactobacillus paracasei LPC12, Lactobacillus rhamnosus LRH10, Lactobacillus fermentum LF26, Lactobacillus helveticus LH43, Streptococcus thermophilus ST30, Lactobacillus plantarum LP23, Lactobacillus helveticus LA27, and Lactobacillus rhamnosus LRH113.

[0011] The first embedding layer includes edible corn starch.

[0012] The second embedding layer includes soy protein.

[0013] The preparation method of the soy protein comprises the following steps:

[0014] (1) Soak soybeans in 3-5 times the mass of water for 15-20 hours, grind 10 kg of the soaked soybeans with a grinding wheel to make pulp, adjust the grinding wheel gap to 0.5 mm, control the pulp temperature to ≤50°C, add 80-100 kg of water during pulping, centrifuge the pulp at a speed of 1500-1800 r / min for 2-4 minutes, and collect the supernatant;

[0015] (2) adjusting the pH of the supernatant to 4.5-4.8 using 1-2 mol / L hydrochloric acid to precipitate the protein, centrifuging, and collecting the protein precipitate; adding the protein precipitate to water 4-5 times the weight of the protein precipitate, and adjusting the pH to 7.0 using sodium hydroxide aqueous solution, stirring and dissolving for 30-40 minutes to dissolve the protein precipitate to obtain a soy protein solution;

[0016] (3) adding a neutral protease to the soy protein solution for enzymolysis, wherein the amount of the enzyme added is 0.4-0.5% of the mass of the soy protein solution, performing enzymolysis at 40-50° C. for 160-200 min, inactivating the enzyme, and obtaining a soy hydrolyzate. The soy hydrolyzate is centrifuged at 2500 rpm for 15 min, and the hydrolyzate is collected and spray-dried to obtain hydrolyzed soy protein.

[0017] (4) dissolving the hydrolyzed soy protein in water 5-7 times the weight of the hydrolyzed soy protein, heating to 50-55° C., fully dissolving, and obtaining a fermentation material; cooling the fermentation material to 30-35° C., adding a composite bacterial agent at 1-3% of the mass of the fermentation material for fermentation at 35° C., anaerobic fermentation for 20-25 hours, sterilizing, and spray drying to obtain the soy protein.

[0018] Preferably, the composite bacterial agent comprises Acetobacter pasteurianus, Streptococcus faecalis and Lactobacillus gasseri in a mass ratio of 1:(1.2-1.5):(0.5-0.7).

[0019] The present invention further ferments the hydrolyzed protein using a specific bacterial agent, which can produce rich peptides and amino acids during the fermentation process. These small molecular weight protein fragments have better solubility and emulsification properties, help form a more uniform and stable embedding structure, and thus provide a more effective protective barrier. At the same time, the fermented soy protein can enhance biocompatibility and cell affinity, can better adhere to or wrap probiotics, reduce mechanical damage during processing, and better resist the effects of harsh environments such as gastric acid in the digestive tract.

[0020] The third embedding layer comprises an enteric material.

[0021] The enteric material comprises Eudragit L30D-55, cellulose acetate phthalate and polyacrylic acid resin II.

[0022] The enteric material also includes a plasticizer.

[0023] The plasticizer is triethyl citrate.

[0024] The plasticizer accounts for 15-20% of the mass of the enteric material.

[0025] The probiotic core also includes at least one of a prebiotic and a nutrient.

[0026] Preferably, the prebiotics include at least one of fructooligosaccharide and isomaltooligosaccharide syrup.

[0027] Preferably, the nutrient is skim milk powder.

[0028] A second aspect of the present invention provides a method for preparing probiotic granules for improving intestinal function, comprising the following steps:

[0029] (1) embedding the probiotic kernel with edible corn starch to obtain first embedded particles;

[0030] (2) embedding the first embedded particles with soybean protein to obtain second embedded particles;

[0031] (3) Enteric-soluble materials are used to encapsulate the surface of the second encapsulated particles to obtain probiotic particles that improve intestinal function.

[0032] Preferably, the diameter of the probiotic core is 200-250 μm, the thickness of the first embedding layer is 30-60 μm, the thickness of the second embedding layer is 10-15 μm, and the thickness of the third embedding layer is 5-10 μm.

[0033] Preferably, in step (1), edible corn starch and water are mixed with a mass concentration of edible corn starch of 15-20% to obtain a first embedding solution, embedding is performed, and drying is performed so that the water content of the first embedding layer is ≤2wt%, to obtain first embedded particles.

[0034] In the step (2), soy protein is added to a 0.01-0.05 mol / L NaOH aqueous solution with a soy protein concentration of 5-10 (w / v)% to obtain a second embedding solution, which is then embedded and dried until the water content of the second embedding layer is ≤2 wt%.

[0035] Preferably, cellulose acetate phthalate is dissolved in an acetone-water (8:2, v / v) mixed solvent to a cellulose acetate phthalate concentration of 8-10 wt% to obtain a CAP solution, which is then stirred for 30 minutes. Polyacrylic acid resin II is dissolved in a 95% ethanol aqueous solution to a mass concentration of 6-8% to obtain a polyacrylic acid resin II ethanol solution. The CAP solution is slowly dripped into Eudragit L30D-55, triethyl citrate is added, and finally the polyacrylic acid resin II ethanol solution is added to obtain a third embedding solution, which is then embedded and dried until the water content of the third embedding layer is ≤2 wt%.

[0036] Preferably, the volume ratio of the CAP solution, Eudragit L30D-55 and the polyacrylic acid resin II ethanol solution is (0.3-0.5):(1.4-1.6):1.

[0037] The present invention adopts a three-layer embedding structure to protectively encapsulate probiotics, which is composed of a first embedding layer (edible corn starch), a second embedding layer (soy protein) and a third embedding layer (enteric material). Through the synergistic effect of different functional materials, this structure improves the storage stability of probiotics at room temperature while enhancing their tolerance in the digestive tract, significantly improving the biological activity and functionality of the product.

[0038] A third aspect of the present invention provides a use of probiotic particles for improving intestinal function.

[0039] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0040] 1. The present invention provides probiotic granules for improving intestinal function with excellent stability and digestive tract tolerance. Using the encapsulation technology described herein, the probiotic granules retain over 80% of active bacteria during processing, significantly reducing the loss of viable bacteria. The encapsulation efficiency significantly surpasses existing technologies, demonstrating enhanced bioactivity retention.

[0041] 2. Under normal temperature and high temperature storage conditions, the probiotic granules of the present invention have a high bacterial viability retention rate, far exceeding most similar products on the market; they have excellent physical and chemical stability and shelf life guarantee capabilities. This feature not only extends the shelf life of the product, but also effectively reduces the cost of cold chain transportation and storage, and broadens the product's application range and market adaptability.

[0042] 3. The probiotic granules of this invention exhibit excellent acid resistance in gastric fluid, effectively resisting the effects of gastric acid and other digestive fluids, ensuring that a sufficient number of active probiotics reach the intestines, regulating intestinal flora, enhancing intestinal barrier function, and promoting nutrient absorption. This product is suitable for the elderly, people with compromised immunity, and ordinary consumers with increased health awareness, and has broad market prospects and social value. DETAILED DESCRIPTION

[0043] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0044] The raw materials used in the following examples of the present invention are all commercially available commodities:

[0045] Acetobacter pasteurianus, product number: ZKCC12083, Beijing Zhongke Quality Inspection Biotechnology Co., Ltd.

[0046] Streptococcus faecalis, product number: HZB115945, Wuhan Huizao Biotechnology Co., Ltd. Hangzhou Branch.

[0047] Lactobacillus gasseri, product number: ZKCC12372, Beijing Zhongke Quality Inspection Biotechnology Co., Ltd.

[0048] Neutral protease, product number S10013, Shanghai Yuanye Biotechnology Co., Ltd.

[0049] Lactobacillus plantarum LP28, Bifidobacterium animalis BAL06, Lactobacillus acidophilus LA1063, Bifidobacterium longum BL986, Lactobacillus paracasei LPC12, Lactobacillus rhamnosus LRH10, Lactobacillus fermentum LF26, Lactobacillus helveticus LH43, Streptococcus thermophilus ST30, Lactobacillus plantarum LP23, Lactobacillus helveticus LA27, and Lactobacillus rhamnosus LRH113 were purchased from Taiwan Shenghe Biotechnology Co., Ltd.

[0050] Example 1

[0051] This embodiment provides a probiotic particle for improving intestinal function, including a probiotic core, a first embedding layer, a second embedding layer and a third embedding layer; the diameter of the probiotic core is 220 μm, the thickness of the first embedding layer is 50 μm, the thickness of the second embedding layer is 12 μm, and the thickness of the third embedding layer is 8 μm.

[0052] The probiotic core includes Lactobacillus plantarum LP28, Bifidobacterium animalis BAL06, Lactobacillus acidophilus LA1063, Bifidobacterium longum BL986, Lactobacillus paracasei LPC12, Lactobacillus rhamnosus LRH10, Lactobacillus fermentum LF26, Lactobacillus helveticus LH43, Streptococcus thermophilus ST30, Lactobacillus plantarum LP23, Lactobacillus helveticus LA27, and Lactobacillus rhamnosus LRH113 in a ratio of 1:1.4:0.2:0.7:1.1:1.5:0.9:0.3:0.8:1.8:0.4:1.6. The total viable bacterial count of the probiotics added to the probiotic granules is 100 billion CFU / g.

[0053] The first embedding layer comprises edible corn starch; the second embedding layer comprises soy protein; and the third embedding layer comprises an enteric material. The enteric material comprises Eudragit L30D-55, cellulose acetate phthalate, and polyacrylic acid resin II. The enteric material also includes a plasticizer, which is triethyl citrate. The plasticizer accounts for 18% of the enteric material by weight.

[0054] The preparation method of the soy protein comprises the following steps:

[0055] (1) Soak soybeans in 4 times their mass of water for 18 h. Then, grind 10 kg of the soaked soybeans with a grinding wheel to make pulp. The grinding wheel gap was adjusted to 0.5 mm. The slurry temperature was controlled at 50°C. The amount of water added during pulping was 80 kg. The slurry was centrifuged at 1600 r / min for 3 min, and the supernatant was collected.

[0056] (2) The supernatant was adjusted to pH 4.6 using 1 mol / L hydrochloric acid to precipitate the protein, and the protein precipitate was collected by centrifugation; the protein precipitate was added to water 4 times the weight of the protein precipitate, and the pH was adjusted to 7.0 using 1 mol / L sodium hydroxide aqueous solution, and the solution was stirred for 35 minutes to dissolve the protein precipitate to obtain a soy protein solution;

[0057] (3) adding neutral protease to the soy protein solution for enzymatic hydrolysis, the enzyme amount being 0.45% of the mass of the soy protein solution, performing enzymatic hydrolysis at 45° C. for 180 min, inactivating the enzyme to obtain a soy hydrolyzate, centrifuging the soy hydrolyzate at 4° C. at 2500 rpm for 15 min, collecting the hydrolyzate, and spray drying to obtain hydrolyzed soy protein;

[0058] (4) Dissolving the hydrolyzed soy protein in water 6 times the weight of the hydrolyzed soy protein, heating to 52° C., fully dissolving, and obtaining a fermentation material; cooling the fermentation material to 35° C., adding a composite bacterial agent of 2% by weight of the fermentation material for fermentation, wherein the composite bacterial agent comprises Acetobacter pasteurianus, Streptococcus faecalis, and Lactobacillus gasseri in a mass ratio of 1:1.3:0.6; fermenting under the conditions of 35° C., anaerobic fermentation for 22 h, sterilization, and spray drying to obtain soy protein.

[0059] A method for preparing probiotic granules for improving intestinal function comprises the following steps:

[0060] (1) Mix edible corn starch and water to obtain a first embedding solution with a mass concentration of 18% of edible corn starch, and embed the probiotic kernels using a centrifugal pelletizer. Maintain the drum temperature of the pelletizer at 30°C, and add an amount of edible corn starch each time so that the pellets can be evenly attached and rotated. Dry the first embedding layer to obtain a water content of 1.2 wt% to obtain first embedded particles.

[0061] (2) Soy protein was added to a 0.02 mol / L NaOH aqueous solution to obtain a 7 (w / v)% soy protein concentration to obtain a second embedding solution, which was then embedded on the surface of the first embedded particles using the same method as in step (1). The solution was dried until the water content of the second embedding layer reached 1 wt%, thereby obtaining second embedded particles.

[0062] (3) Cellulose acetate phthalate was dissolved in a mixed solvent of acetone and water (8:2, v / v) to a concentration of 9 wt% to obtain a CAP solution, which was stirred for 30 min. Polyacrylic acid resin II was dissolved in a 95% ethanol aqueous solution to a mass concentration of 7% to obtain a polyacrylic acid resin II ethanol solution. The CAP solution was slowly dripped into Eudragit L30D-55, triethyl citrate was added, and finally the polyacrylic acid resin II ethanol solution was added to obtain a third embedding solution. The volume ratio of the CAP solution, Eudragit L30D-55 and the polyacrylic acid resin II ethanol solution was 0.4:1.5:1. The second embedding particles were embedded in the surface using the same method as step (1). The particles were dried until the water content of the third embedding layer was 1 wt% to obtain probiotic particles that improved intestinal function.

[0063] Example 2

[0064] The difference between this embodiment and embodiment 1 is that the composite bacterial agent comprises Acetobacter pasteurianus, Streptococcus faecalis and Lactobacillus gasseri in a mass ratio of 1:1.2:0.7.

[0065] Example 3

[0066] The difference between this embodiment and embodiment 1 is that the composite bacterial agent comprises Acetobacter pasteurianus, Streptococcus faecalis and Lactobacillus gasseri in a mass ratio of 1:1.5:0.5.

[0067] Example 4

[0068] The difference between this embodiment and embodiment 1 is that the volume ratio of the CAP solution, Eudragit L30D-55 and the polyacrylic acid resin II ethanol solution is 0.3:1.6:1.

[0069] Example 5

[0070] The difference between this embodiment and embodiment 1 is that the volume ratio of the CAP solution, Eudragit L30D-55 and the polyacrylic acid resin II ethanol solution is 0.5:1.4:1.

[0071] Comparative Example 1

[0072] The difference between this comparative example and Example 1 is that the soy protein is a commercially available product purchased from Anhui Youtai Bioengineering Co., Ltd.

[0073] Comparative Example 2

[0074] The difference between this comparative example and Example 1 is that the soy protein is a commercially available product purchased from Jiangsu Weizhirun Biotechnology Co., Ltd.

[0075] Comparative Example 3

[0076] The difference between this comparative example and Example 1 is that the composite bacterial agent includes Acetobacter pasteurianus, Streptococcus faecalis and Lactobacillus gasseri in a mass ratio of 1.3:0.6:1.

[0077] Comparative Example 4

[0078] The difference between this comparative example and Example 1 is that the composite bacterial agent includes Acetobacter pasteurianus, Streptococcus faecalis and Lactobacillus gasseri in a mass ratio of 0.6:1:1.3.

[0079] Comparative Example 5

[0080] The difference between this comparative example and Example 1 is that the composite bacterial agent comprises Lactobacillus plantarum, Lactobacillus acidophilus and Bifidobacterium longum in a mass ratio of 1:1.3:0.6.

[0081] Comparative Example 6

[0082] The difference between this comparative example and Example 1 is that the composite bacterial agent includes Lactobacillus helveticus, Streptococcus thermophilus and Lactobacillus rhamnosus in a mass ratio of 1:1.3:0.6.

[0083] Comparative Example 7

[0084] The difference between this comparative example and Example 1 is that the volume ratio of the CAP solution, Eudragit L30D-55 and the polyacrylic acid resin II ethanol solution is 1.5:1:0.4.

[0085] Comparative Example 8

[0086] The difference between this comparative example and Example 1 is that the volume ratio of the CAP solution, Eudragit L30D-55 and the polyacrylic acid resin II ethanol solution is 1:0.4:1.5.

[0087] Performance Testing

[0088] The probiotic granules prepared in Examples 1-5 and Comparative Examples 1-8 were subjected to performance tests.

[0089] (1) Long-term storage test: The probiotic granules were packed in nitrogen-filled aluminum-plastic packaging and stored at room temperature (25 ± 2 °C) for 6 months, and the number of viable bacteria was determined.

[0090] (2) Accelerated test: The probiotic granules were placed in an environment of 37°C and 50% relative humidity, and the number of viable bacteria was detected after 2 months.

[0091] (3) Artificial gastric fluid test: In an artificial gastric fluid environment at 37°C (prepared according to the pharmacopoeia method, pH 2), the number of viable bacteria was detected after 60 minutes.

[0092] Table 1 Performance test results (unit: 100 million CFU / g)

[0093]

[0094]

[0095] As shown in Table 1, the probiotic granules of Examples 1-5 and Comparative Examples 1-8 retained over 80% of their bacterial activity after encapsulation. The bacterial activity retention of Examples 1-5 exceeded 40% after six months of storage at room temperature, exceeded 50% after accelerated testing, and exceeded 80% in gastric fluid. The overall results are excellent, significantly superior to existing technologies.

[0096] Comparative Examples 1-2 show that the stability of probiotic granules decreases when commercially available soy protein is used. Comparative Examples 4-6 show that the type and ratio of the composite bacterial agent used to prepare soy protein will affect the final composition of the soy protein, and thus affect the stability of the probiotic granules.

[0097] Comparative Examples 7 and 8 show that the ratio of Eudragit L30D-55, cellulose acetate phthalate, and polyacrylic acid resin II in the enteric material also has an important influence on the stability of the probiotic granules.

[0098] The above results indicate that the layer encapsulation methods used in the present invention have a synergistic effect and can jointly improve the stability of the probiotic particle size.

[0099] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A probiotic granule for improving intestinal function, characterized in that: The invention comprises a probiotic core, a first embedding layer, a second embedding layer and a third embedding layer; the probiotic core comprises Lactobacillus plantarum, Bifidobacterium animalis, Lactobacillus acidophilus, Bifidobacterium longum, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus fermentum, Lactobacillus helveticus, Streptococcus thermophilus, Lactobacillus plantarum, Lactobacillus helveticus and Lactobacillus rhamnosus.

2. The probiotic granules for improving intestinal function according to claim 1, characterized in that The first embedding layer includes edible corn starch.

3. The probiotic granules for improving intestinal function according to claim 1, characterized in that The second embedding layer includes soy protein.

4. The probiotic granules for improving intestinal function according to claim 3, characterized in that The preparation method of the soy protein comprises the following steps: (1) Soaking soybeans in water, pulping the soaked soybeans, centrifuging the pulp, and collecting the supernatant; (2) adjusting the pH of the supernatant to 4.5-4.8 to precipitate the protein, centrifuging, and collecting the protein precipitate; adding the protein precipitate to water, adjusting the pH to 7.0, stirring and dissolving the protein precipitate to obtain a soy protein solution; (3) adding neutral protease to the soybean protein solution for enzymolysis, inactivating the enzyme to obtain a soybean hydrolyzate, centrifuging the soybean hydrolyzate, collecting the hydrolyzate, and spray drying to obtain hydrolyzed soybean protein; (4) dissolving the hydrolyzed soybean protein in water, heating it to fully dissolve it, and obtaining a fermentation material; cooling the fermentation material, adding a composite bacterial agent to ferment it, sterilizing it, and spray drying it to obtain soybean protein.

5. The probiotic granules for improving intestinal function according to claim 4, characterized in that The composite bacterial agent comprises Acetobacter pasteurianus, Streptococcus faecalis and Lactobacillus gasseri in a mass ratio of 1: (1.2-1.5): (0.5-0.7).

6. The probiotic granules for improving intestinal function according to claim 1, characterized in that The third embedding layer comprises an enteric material.

7. The probiotic granules for improving intestinal function according to claim 6, characterized in that The enteric material comprises Eudragit L30D-55, cellulose acetate phthalate and polyacrylic acid resin II.

8. A method for preparing a probiotic granule for improving intestinal function according to any one of claims 1 to 7, characterized in that: The following steps are involved: (1) embedding the probiotic kernel with edible corn starch to obtain first embedded particles; (2) embedding the first embedded particles with soybean protein to obtain second embedded particles; (3) Enteric-soluble materials are used to encapsulate the surface of the second encapsulated particles to obtain probiotic particles that improve intestinal function.

9. The method for preparing probiotic granules for improving intestinal function according to claim 8, characterized in that: Cellulose acetate phthalate was dissolved in an acetone-water mixed solvent to obtain a CAP solution, which was then stirred continuously. Polyacrylic acid resin II was dissolved in an ethanol aqueous solution to obtain a polyacrylic acid resin II ethanol solution. The CAP solution was dropped into Eudragit L30D-55, and the polyacrylic acid resin II ethanol solution was added to obtain a third embedding solution for embedding.

10. Use of the probiotic granules for improving intestinal function according to any one of claims 1 to 9 in improving intestinal function.

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