A carbapenem resistance gene detection device and detection method

Abstract

This invention belongs to the field of gene detection technology, specifically a carbapenem resistance gene detection device and method. It includes a detector with a detection chamber and a detection unit within the chamber. The detection unit comprises a pneumatic control module, an optical detection module, and a constant temperature module. A sealing cover is provided on the top surface of the detector to seal the detection chamber. By placing a microfluidic chip onto a suction cup and pressing it to adhere, the sealing cover is closed. Then, an electric telescopic rod moves downwards, causing a first spring to push a connecting plate, which in turn moves the suction cup downwards. The suction cup then moves the chip downwards until its bottom surface contacts the inner wall of the groove. Finally, the electric telescopic rod is closed, thus fixing the microfluidic chip within the groove. The suction cup can be used to fix microfluidic chips of different models and sizes.

Description

Technical Field

[0001] This invention belongs to the field of gene detection technology, specifically a carbapenem resistance gene detection device and detection method. Background Technology

[0002] Currently, bacterial resistance has become a major challenge in the global public health field, with infections caused by carbapenem-resistant Enterobacterales (CRE) being particularly severe. The production of carbapenemases is the most important mechanism of resistance to carbapenems in Enterobacterales. Accurate and rapid detection and typing of carbapenemases produced by CREs are of great value for precise drug use in clinical anti-infective therapy and prevention and control of hospital infections.

[0003] Microfluidic chip technology has advantages such as speed, simplicity, high specificity, high sensitivity, and high throughput. It can be applied to the identification of bacteria. This technology is used to analyze KPC, NDM, IMP, and VIM resistance genes, and the sequencing results are used as the gold standard to calculate specificity and sensitivity, providing data for clinical anti-infective treatment.

[0004] When in use, the microfluidic chip needs to be placed in a detection chamber, which integrates three major modules: pneumatic control, optical detection, and thermostat. The pneumatic system precisely regulates the flow of the sample fluid (carbapenem) within the chip; the optical module is responsible for exciting fluorescence signals and collecting detection data; and the thermostat module maintains the reaction temperature to ensure isothermal amplification of the sample. All modules work together to achieve efficient and accurate detection and analysis.

[0005] However, the above technologies often have the following drawbacks: Currently, microfluidic chips come in various sizes and models, but in actual use, the chips need to be placed in the chip slots in the detection chamber. The chip slots are often difficult to fit chips of different specifications. Since the chip slots are usually of a fixed size, when the chip size is too small or too large, it will be impossible to fix the chip, thus affecting the limitations of the detection equipment. To this end, the present invention provides a carbapenem resistance gene detection device and detection method. Summary of the Invention

[0006] In order to overcome the shortcomings of the prior art, at least one technical problem raised in the background art is solved.

[0007] The technical solution adopted by the present invention to solve its technical problem is as follows: A carbapenem resistance gene detection device of the present invention includes a detector, a detection chamber is provided inside the detector, a detection unit is provided inside the detection chamber, the detection unit includes a pneumatic control module, an optical detection module and a constant temperature module, a sealing cover for sealing the detection chamber is provided on the top surface of the detector; a detection platform is fixedly connected to the bottom surface of the inner wall of the detection chamber, a groove is opened on the top surface of the detection platform, a cavity is opened inside the detection platform, a connecting plate is provided inside the cavity; a set of connecting holes communicating with the cavity and the groove are opened on the detection platform, a connecting plate is provided inside the cavity, a set of connecting rods passing through the connecting holes are fixedly connected to the top surface of the connecting plate, a suction cup is fixedly connected to the top surface of the connecting rods, an electric telescopic rod for pushing the connecting plate is fixedly connected to the bottom surface of the inner wall of the cavity, a set of first springs is fixedly connected between the top surface of the connecting plate and the inner wall of the cavity, and a microfluidic chip is provided on the suction cup.

[0008] Preferably, the detection platform has a pair of connecting grooves, the inner wall of the cavity has a communicating air inlet between it and the connecting grooves, the inner wall of the groove has multiple sets of air outlets communicating with the connecting grooves, and the connecting plate is slidably connected to the inner wall of the cavity in a sealed manner.

[0009] Preferably, a pair of limiting blocks are slidably connected inside the detection platform. The bottom surface of the limiting block is located inside the cavity, the top surface of the limiting block is located inside the groove, and a fixing plate is fixedly connected to the outer wall of the limiting block. A driving spring is fixedly connected between the top surface of the fixing plate and the inner wall of the cavity.

[0010] Preferably, the constant temperature module includes a set of constant temperature rods disposed in the cavity, the constant temperature rods being located above the connecting plate, and an elastic sealing ring being fixedly connected to the inner wall of the connecting hole.

[0011] Preferably, the detection platform has multiple sets of sliding grooves corresponding to the sealing ring. A magnetic block is slidably connected in the sliding groove. A magnetic plate that repels the magnetic block is slidably connected to the inner wall of the sliding groove. A second spring is fixedly connected between the side of the magnetic plate away from the sealing ring and the inner wall of the sliding groove. A connecting line is fixedly connected between the bottom surface of the magnetic plate and the connecting plate. A through groove communicating with the sliding groove is opened on the inner wall of the connecting hole. The sealing ring has a hollow structure inside. A through hole corresponding to the through groove is opened on the outer wall of the sealing ring. A positioning plate is fixedly connected to the inner wall of the cavity.

[0012] Preferably, the top surface of the connecting plate is provided with a set of rotatable shafts, the outer side wall of the shafts is fixedly connected with a set of drive blades, and the connecting plate is provided with a drive assembly for driving the shafts to rotate.

[0013] Preferably, the drive assembly includes a rectangular groove formed on the bottom surface of the connecting plate, a rectangular plate disposed in the rectangular groove, the output end of the electric telescopic rod being fixedly connected to the rectangular plate, the rotating shaft being slidably connected to the top surface of the connecting plate, and the bottom end of the rotating shaft being rotatably connected to the top surface of the rectangular plate.

[0014] Preferably, the connecting rod has a hollow internal structure, the top end of the connecting rod communicates with the suction cup, the top surface of the connecting plate has a circular hole for communicating with the connecting rod, a piston is slidably connected inside the connecting rod, a first elastic rope is fixedly connected between the bottom surface of the piston and the inner wall of the connecting rod, a second elastic rope is fixedly connected between the top surface of the piston and the inner wall of the connecting rod, the elasticity of the second elastic rope is greater than that of the first elastic rope, and a limit ring is fixedly connected to the inner wall of the connecting rod.

[0015] A method for detecting carbapenem resistance genes, the method employing the aforementioned carbapenem resistance gene detection device, and the method comprising the following steps:

[0016] S1: By injecting the carbapenem sample into the microfluidic chip, opening the sealing cover, placing the microfluidic chip on the limiting block, and then controlling the connecting plate to move up and down with the help of the output end of the electric telescopic rod and the first spring, the connecting plate pushes the gas in the cavity to be sprayed from the air outlet onto the microfluidic chip.

[0017] S2: Press the microfluidic chip to adhere to the suction cup, and then move the output end of the electric telescopic rod downward. At this time, the first spring will push the connecting plate, so that the connecting plate drives the microfluidic chip on the suction cup to contact the inner wall of the groove.

[0018] S3: The sample is drawn into the microfluidic chip through the pneumatic control module, flows through the chamber pre-stored lysis reagent, and enters the heating zone after mixing. The pneumatic membrane pump drives the mixture through the chip's built-in silica membrane micropillar or magnetic bead capture area. The waste liquid is pumped into the waste liquid chamber. The purified nucleic acid binds to the membrane / magnetic beads, and the washing buffer is pumped in for washing.

[0019] S4: The pneumatic membrane pump precisely delivers the eluted nucleic acid solution to multiple parallel PCR micro-reaction chambers of the microfluidic chip. At the same time, the premixed PCR master mixture is pumped in. The nucleic acid template and reaction reagents are mixed and redissolved in the micro-reaction chamber. The PMT does not work to confirm whether the liquid fills the reaction chamber.

[0020] S5: The cavity is heated by a thermostatic rod, and the drive blade is rotated at the same time, so that the drive blade agitates the gas above the connecting plate until the temperature inside the cavity is heated to 64℃-66℃. At this time, the heat inside the cavity is applied to the microfluidic chip on the groove to provide a constant temperature effect for the microfluidic chip.

[0021] S6: The optical module is responsible for exciting fluorescence signals and collecting detection data to complete the detection of carbapenem resistance genes.

[0022] The beneficial effects of this invention are as follows:

[0023] 1. This invention involves placing a microfluidic chip onto a suction cup and pressing it to adhere to the suction cup. After closing the sealing cover, the electric telescopic rod is moved downwards. At this point, the first spring pushes the connecting plate, causing the connecting plate to move the suction cup downwards. The suction cup then moves the chip downwards, at which point the bottom surface of the chip contacts the inner wall of the groove. The electric telescopic rod is then closed, thereby fixing the microfluidic chip in the groove. Different models and sizes of microfluidic chips can be fixed using the suction cup.

[0024] 2. When the connecting plate in this invention is pushed downward by the first spring, the connecting plate will push the gas in the cavity into the connecting groove from the air inlet. At this time, the gas will be blown out from the air outlet onto the microfluidic chip, thereby blowing away impurities on the microfluidic chip and improving the accuracy of subsequent detection. Attached Figure Description

[0025] The invention will now be further described with reference to the accompanying drawings.

[0026] Figure 1 This is a three-dimensional structural diagram of the detector in this invention;

[0027] Figure 2 This is a schematic diagram of the detector structure after the sealing cover is removed in this invention;

[0028] Figure 3 This is a three-dimensional structural diagram of the detection platform in this invention;

[0029] Figure 4 This is a schematic diagram of the internal structure of the detection platform in this invention;

[0030] Figure 5 yes Figure 4 Enlarged view of point A;

[0031] Figure 6 This is a schematic diagram of the internal structure of the rectangular plate and connecting rod in this invention;

[0032] Figure 7 This is a flowchart of the method in this invention.

[0033] In the diagram: 1. Detector; 2. Sealing cap; 3. Detection chamber; 4. Detection platform; 5. Groove; 6. Microfluidic chip; 7. Cavity; 8. Connecting plate; 9. Connecting rod; 10. Suction cup; 11. Electric telescopic rod; 12. Connecting groove; 13. Air outlet; 14. Air inlet; 15. Limiting block; 16. Drive spring; 17. Thermostatic rod; 18. Sealing ring; 19. Positioning plate; 20. Magnetic block; 21. Through groove; 22. Magnetic plate; 23. Connecting wire; 24. Rotating shaft; 25. Drive blade; 26. Rectangular plate; 27. Rectangular groove; 28. First elastic rope; 29. ​​Limiting ring; 30. Piston; 31. Second elastic rope; 32. Slide groove. Detailed Implementation

[0034] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0035] Example 1: As Figures 1 to 5 As shown in the embodiment of the present invention, a carbapenem resistance gene detection device includes a detector 1, a detection chamber 3, and a detection unit, which includes a pneumatic control module, an optical detection module, and a constant temperature module. A sealing cover 2 for sealing the detection chamber 3 is provided on the top surface of the detector 1. A detection platform 4 is fixedly connected to the bottom surface of the inner wall of the detection chamber 3. A groove 5 is formed on the top surface of the detection platform 4, and a cavity 7 is formed inside the detection platform 4. A connecting plate 8 is provided inside the cavity 7; a set of connecting holes communicating with the cavity 7 and the groove 5 are provided on the detection platform 4; a connecting plate 8 is provided inside the cavity 7; a set of connecting rods 9 passing through the connecting holes are fixedly connected to the top surface of the connecting plate 8; a suction cup 10 is fixedly connected to the top surface of the connecting rods 9; an electric telescopic rod 11 for pushing the connecting plate 8 is fixedly connected to the bottom surface of the inner wall of the cavity 7; a set of first springs is fixedly connected between the top surface of the connecting plate 8 and the inner wall of the cavity 7; and a microfluidic chip 6 is provided on the suction cup 10.

[0036] In this application, when detecting carbapenem resistance genes, a carbapenem sample can be injected into a microfluidic chip 6, then the microfluidic chip 6 is placed on a suction cup 10 and pressed to adhere to the suction cup 10. After that, the sealing cover 2 is closed, and the electric telescopic rod 11 is moved downward. At this time, the first spring pushes the connecting plate 8, causing the connecting plate 8 to move the suction cup 10 downward. The suction cup 10 then moves the chip downward, and the bottom surface of the chip contacts the inner wall of the groove 5. Then the electric telescopic rod 11 is closed, thereby fixing the microfluidic chip 6 in the groove 5. The suction cup 10 can fix microfluidic chips 6 of different models and sizes, thereby improving the practicality of the entire device. Then, the pneumatic control module controls the flow of the sample in the microfluidic chip 6, and the optical detection module keeps the microfluidic chip 6 at a constant temperature. The optical module is responsible for exciting fluorescence signals and collecting detection data to complete the detection of carbapenem resistance genes.

[0037] The detection platform 4 has a pair of connecting grooves 12. The inner wall of the cavity 7 and the connecting grooves 12 are connected by an air inlet 14. The inner wall of the groove 5 has multiple sets of air outlets 13 connected to the connecting grooves 12. The connecting plate 8 is slidably connected to the inner wall of the cavity 7 in a sealed manner. In this application, when the connecting plate 8 is pushed downward by the first spring, the connecting plate 8 will push the gas in the cavity 7 into the connecting groove 12 through the air inlet 14. At this time, the gas will be blown out from the air outlet 13 onto the microfluidic chip 6, thereby blowing away impurities on the microfluidic chip 6 to improve the accuracy of subsequent detection.

[0038] A pair of limiting blocks 15 are slidably connected inside the detection platform 4. The bottom surface of the limiting block 15 is located inside the cavity 7, and the top surface of the limiting block 15 is located inside the groove 5. A fixing plate is fixedly connected to the outer wall of the limiting block 15, and a driving spring 16 is fixedly connected between the top surface of the fixing plate and the inner wall of the cavity 7. When the microfluidic chip 6 in this application is placed in the groove 5, the microfluidic chip 6 will first contact the limiting block 15. At this time, the output end of the electric telescopic rod 11 can be moved up and down repeatedly. When the output end moves down, the first spring will push the connecting plate 8, so that the connecting plate 8 pushes the gas in the cavity 7, so that the gas can be sprayed out from the air outlet 13. When the output end moves up, it can push the connecting plate 8 to reset. During the back and forth movement of the output end, the air outlet 13 can continuously emit air, thereby continuously blowing and cleaning the microfluidic chip 6 to improve the cleaning effect of the microfluidic chip 6.

[0039] The constant temperature module includes a set of constant temperature rods 17 disposed in the cavity 7. The constant temperature rods 17 are located above the connecting plate 8. An elastic sealing ring 18 is fixedly connected to the inner wall of the connecting hole. Before the test, the microfluidic chip 6 is first adsorbed onto the suction cup 10, and the bottom surface of the microfluidic chip 6 is made to contact the inner wall of the groove 5. At the same time, the sealing ring 18 will contact and seal with the suction cup 10, so that the cavity 7 is in a sealed state. Then, the cavity 7 can be heated with the help of a heating rod until the temperature inside the cavity 7 is heated to 64-66°C. At this time, the heat inside the cavity 7 acts on the microfluidic chip 6 on the groove 5 to provide a constant temperature effect for the microfluidic chip 6, thereby providing the effect of gene detection.

[0040] The detection platform 4 has multiple sets of sliding grooves 32 corresponding to the sealing ring 18. A magnetic block 20 is slidably connected in the sliding groove 32. A magnetic plate 22 that repels the magnetic block 20 is slidably connected to the inner wall of the sliding groove 32. A second spring is fixedly connected between the side of the magnetic plate 22 away from the sealing ring 18 and the inner wall of the sliding groove 32. A connecting line 23 is fixedly connected between the bottom surface of the magnetic plate 22 and the connecting plate 8. A through groove 21 communicating with the sliding groove 32 is opened on the inner wall of the connecting hole. The sealing ring 18 has a hollow structure inside. A through hole corresponding to the through groove 21 is opened on the outer wall of the sealing ring 18. A positioning plate 19 is fixedly connected to the inner wall of the cavity 7.

[0041] When the connecting plate 8 moves downward, it will drive the suction cup 10 downward, so that the suction cup 10 enters the connecting hole and contacts the sealing ring 18. At the same time, the connecting plate 8 will contact the positioning. Simultaneously, the connecting line 23 will also pull the magnetic plate 22 downward, so that the magnetic plate 22 moves away from the front of the magnetic block 20. At this time, the magnetic block 20 will move under the push of the second spring, so that the gas in the slide groove 32 enters the sealing ring 18 through the through groove 21, thereby causing the sealing ring 18 to expand and further fit with the suction cup 10 to improve the sealing effect of the cavity 7. By controlling the connecting plate 8 to move upward through the electric telescopic rod 11, the connecting plate 8 can push the magnetic plate 22 to reset. At this time, the magnetic block 20 will reset due to the repulsion between it and the magnetic plate 22.

[0042] Example 2: Figure 6As shown in the comparative embodiment one, another embodiment of the present invention is as follows: a set of rotatable shafts 24 are provided on the top surface of the connecting plate 8, a set of driving blades 25 are fixedly connected to the outer wall of the shafts 24, and a driving assembly for driving the shafts 24 to rotate is provided on the connecting plate 8; when the thermostat rod 17 heats the cavity 7, the driving assembly can drive the shafts 24 to rotate, at which time the driving blades 25 will rotate, so that the driving blades 25 agitate the gas located above the connecting plate 8, thereby improving the efficiency of the thermostat rod 17 in heating the gas. At the same time, because of the agitation of the gas, the gas can also be better kept in a uniform temperature state, thereby improving the thermostat effect of the microfluidic chip 6.

[0043] The drive assembly includes a rectangular groove 27 formed on the bottom surface of the connecting plate 8, a rectangular plate 26 disposed within the rectangular groove 27, the output end of the electric telescopic rod 11 being fixedly connected to the rectangular plate 26, the rotating shaft 24 being slidably connected to the top surface of the connecting plate 8, and the bottom end of the rotating shaft 24 being rotatably connected to the top surface of the rectangular plate 26; after the connecting plate 8 contacts the positioning plate 19, the output end of the electric telescopic rod 11 can control the rectangular plate 26 to continue moving up and down. During the up and down movement of the rectangular plate 26, the drive blade 25 is pushed to rotate by the gas in the cavity 7, thereby causing the drive blade 25 to rotate and agitate and mix the gas in the cavity 7.

[0044] The connecting rod 9 has a hollow internal structure. The top end of the connecting rod 9 communicates with the suction cup 10. The top surface of the connecting plate 8 has a circular hole communicating with the connecting rod 9. A piston 30 is slidably connected inside the connecting rod 9. A first elastic rope 28 is fixedly connected between the bottom surface of the piston 30 and the inner wall of the connecting rod 9. A second elastic rope 31 is fixedly connected between the top surface of the piston 30 and the inner wall of the connecting rod 9. The elastic force of the second elastic rope 31 is greater than that of the first elastic rope 28. A limiting ring 29 is fixedly connected to the inner wall of the connecting rod 9. During the downward movement of the rectangular plate 26 in this application, the first elastic rope 28 will pull the piston 30 downward. At this time, the piston 30 will draw gas from the suction cup 10, making the suction cup 10 more firmly adsorb the microfluidic chip 6, thereby improving the fixation effect of the microfluidic chip 6. Afterward, when the rectangular plate 26 continues to move downward, the limiting ring 29 will limit the piston 30.

[0045] like Figure 7 As shown, a method for detecting carbapenem resistance genes is described above. This method uses the aforementioned carbapenem resistance gene detection device and includes the following steps:

[0046] S1: By injecting the carbapenem sample into the microfluidic chip 6, then opening the sealing cover 2, and then placing the microfluidic chip 6 on the limiting block 15, the connecting plate 8 is moved up and down by means of the output end of the electric telescopic rod 11 and the cooperation of the first spring, so that the connecting plate 8 pushes the gas in the cavity 7 to be sprayed from the air outlet 13 onto the microfluidic chip 6.

[0047] S2: Press the microfluidic chip 6 to adhere to the suction cup 10, and then let the output end of the electric telescopic rod 11 move downward. At this time, the first spring will push the connecting plate 8, so that the connecting plate 8 drives the microfluidic chip 6 on the suction cup 10 to contact the inner wall of the groove 5.

[0048] S3: The sample is drawn from the inlet of the microfluidic chip 6 through the pneumatic control module, flows through the chamber pre-stored lysis reagent, and enters the heating zone after mixing. The pneumatic membrane pump drives the mixture through the chip's built-in silica membrane micropillar or magnetic bead capture area. The waste liquid is pumped into the waste liquid chamber. The purified nucleic acid binds to the membrane / magnetic beads, and the washing buffer is pumped in for washing.

[0049] S4: The pneumatic membrane pump precisely delivers the eluted nucleic acid solution to multiple parallel PCR micro-reaction chambers of the microfluidic chip 6. At the same time, the premixed PCR master mixture is pumped in. The nucleic acid template and reaction reagents are mixed and redissolved in the micro-reaction chamber. The PMT does not work to confirm whether the liquid fills the reaction chamber.

[0050] S5: The cavity 7 is heated by the thermostatic rod 17, and the drive blade 25 is rotated at the same time, so that the drive blade 25 stirs the gas located above the connecting plate 8 until the temperature inside the cavity 7 is heated to 64℃-66℃. At this time, the heat inside the cavity 7 is applied to the microfluidic chip 6 on the groove 5 to provide a constant temperature effect for the microfluidic chip 6.

[0051] S6: The optical module is responsible for exciting fluorescence signals and collecting detection data to complete the detection of carbapenem resistance genes.

[0052] Working principle: A carbapenem sample is injected into the microfluidic chip 6, which is then placed on the suction cup 10 and pressed to adhere to it. The sealing cap 2 is then closed. The electric telescopic rod 11 moves downwards, causing the first spring to push the connecting plate 8, which in turn moves the suction cup 10 downwards. The suction cup 10 then moves the chip downwards, bringing its bottom surface into contact with the inner wall of the groove 5. The electric telescopic rod 11 is then closed, thus fixing the microfluidic chip 6 within the groove 5. The suction cup 10 can be used to fix microfluidic chips 6 of different models and sizes. The device is stabilized to improve its overall usability. The pneumatic control module then controls the flow of the sample within the microfluidic chip 6. The optical detection module maintains a constant temperature for the microfluidic chip 6 and is responsible for exciting fluorescence signals and collecting detection data to complete the detection of carbapenem resistance genes. In this application, when the connecting plate 8 is pushed downwards by the first spring, it pushes the gas in the cavity 7 from the air inlet 14 into the connecting groove 12. The gas is then blown out from the air outlet 13 onto the microfluidic chip 6, thereby removing impurities from the microfluidic chip 6 and improving the accuracy of subsequent detection.

[0053] When the microfluidic chip 6 in this application is placed in the groove 5, the microfluidic chip 6 will first contact the limiting block 15. At this time, the output end of the electric telescopic rod 11 can be moved up and down repeatedly. When the output end moves down, the first spring will push the connecting plate 8, so that the connecting plate 8 pushes the gas in the cavity 7, so that the gas can be ejected from the air outlet 13. When the output end moves up, it can push the connecting plate 8 to reset. During the back and forth movement of the output end, the air outlet 13 can continuously blow air out, thereby continuously cleaning the microfluidic chip 6 and improving the cleaning effect of the microfluidic chip 6.

[0054] Before testing, this application first attaches the microfluidic chip 6 to the suction cup 10, ensuring the bottom surface of the microfluidic chip 6 contacts the inner wall of the groove 5. Simultaneously, the sealing ring 18 seals against the suction cup 10, thus sealing the cavity 7. A heating rod is then used to heat the cavity 7 until the temperature reaches 64-66°C. This heat is then transferred to the microfluidic chip 6 on the groove 5, providing a constant temperature for the microfluidic chip 6 and thus enhancing the gene detection effect. When the connecting plate 8 moves downwards, it causes the suction cup 10 to move downwards, allowing the suction cup 10 to enter the groove 5. Inside the connecting hole, and in contact with the sealing ring 18, the connecting plate 8 will also contact the positioning. At the same time, the connecting line 23 will pull the magnetic plate 22 downward, causing the magnetic plate 22 to move away from the front of the magnetic block 20. At this time, the magnetic block 20 will move under the push of the second spring, causing the gas in the slide groove 32 to enter the sealing ring 18 from the through groove 21, thereby causing the sealing ring 18 to expand and further fit with the suction cup 10 to improve the sealing effect of the cavity 7. By controlling the connecting plate 8 to move upward through the electric telescopic rod 11, the connecting plate 8 can push the magnetic plate 22 to reset. At this time, the magnetic block 20 will reset due to the repulsion between it and the magnetic plate 22.

[0055] The terms "front," "back," "left," "right," "top," and "bottom" all refer to the figures in the accompanying drawings. Figure 1 Based on the perspective of the observer, the side of the device facing the observer is defined as the front, the left side of the observer is defined as the left, and so on.

[0056] In the description of this invention, it should be understood that the terms "center", "longitudinal", "lateral", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting the scope of protection of this invention.

[0057] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A carbapenem resistance gene detection device, comprising a detector (1), wherein a detection chamber (3) is provided in the detector (1), and a detection unit is provided in the detection chamber (3), wherein the detection unit comprises a pneumatic control module, an optical detection module and a constant temperature module, and a sealing cover (2) for sealing the detection chamber (3) is provided on the top surface of the detector (1). Its features are: The bottom surface of the inner wall of the detection cavity (3) is fixedly connected to the detection platform (4), the top surface of the detection platform (4) is provided with a groove (5), the detection platform (4) is provided with a cavity (7), and a connecting plate (8) is provided in the cavity (7). The detection platform (4) is provided with a set of connecting holes communicating with the cavity (7) and the groove (5). A connecting plate (8) is provided in the cavity (7). A set of connecting rods (9) passing through the connecting holes are fixedly connected to the top surface of the connecting plate (8). A suction cup (10) is fixedly connected to the top surface of the connecting rods (9). An electric telescopic rod (11) for pushing the connecting plate (8) is fixedly connected to the bottom surface of the inner wall of the cavity (7). A set of first springs is fixedly connected between the top surface of the connecting plate (8) and the inner wall of the cavity (7). A microfluidic chip (6) is provided on the suction cup (10). The top surface of the connecting plate (8) is provided with a set of rotatable shafts (24), and a set of drive blades (25) are fixedly connected to the outer side wall of the shafts (24). The connecting plate (8) is provided with a drive assembly for driving the shafts (24) to rotate. The drive assembly includes a rectangular groove (27) formed on the bottom surface of the connecting plate (8), a rectangular plate (26) is provided in the rectangular groove (27), the output end of the electric telescopic rod (11) is fixedly connected to the rectangular plate (26), the rotating shaft (24) is slidably connected to the top surface of the connecting plate (8), and the bottom end of the rotating shaft (24) is rotatably connected to the top surface of the rectangular plate (26). The detection platform (4) has a pair of connecting grooves (12) inside. The inner wall of the cavity (7) and the connecting grooves (12) have a communicating air inlet (14). The inner wall of the groove (5) has multiple sets of air outlets (13) communicating with the connecting grooves (12). The connecting plate (8) is in a sealed sliding connection with the inner wall of the cavity (7).

2. The carbapenem resistance gene detection device according to claim 1, characterized in that: A pair of limiting blocks (15) are slidably connected inside the detection platform (4). The bottom surface of the limiting block (15) is located inside the cavity (7), and the top surface of the limiting block (15) is located inside the groove (5). A fixing plate is fixedly connected to the outer wall of the limiting block (15), and a driving spring (16) is fixedly connected between the top surface of the fixing plate and the inner wall of the cavity (7).

3. The carbapenem resistance gene detection device according to claim 2, characterized in that: The constant temperature module includes a set of constant temperature rods (17) disposed in the cavity (7), the constant temperature rods (17) being located above the connecting plate (8), and an elastic sealing ring (18) being fixedly connected to the inner wall of the connecting hole.

4. The carbapenem resistance gene detection device according to claim 3, characterized in that: The detection platform (4) has multiple sets of sliding grooves (32) corresponding to the sealing ring (18). A magnetic block (20) is slidably connected in the sliding groove (32). A magnetic plate (22) that repels the magnetic block (20) is slidably connected to the inner wall of the sliding groove (32). A second spring is fixedly connected between the side of the magnetic plate (22) away from the sealing ring (18) and the inner wall of the sliding groove (32). A connecting line (23) is fixedly connected between the bottom surface of the magnetic plate (22) and the connecting plate (8). A through groove (21) communicating with the sliding groove (32) is opened on the inner wall of the connecting hole. The sealing ring (18) has a hollow structure inside. A through hole corresponding to the through groove (21) is opened on the outer wall of the sealing ring (18). A positioning plate (19) is fixedly connected to the inner wall of the cavity (7).

5. The carbapenem resistance gene detection device according to claim 4, characterized in that: The connecting rod (9) has a hollow structure inside. The top end of the connecting rod (9) is connected to the suction cup (10). The top surface of the connecting plate (8) is provided with a circular hole for the connecting rod (9) to communicate. A piston (30) is slidably connected inside the connecting rod (9). A first elastic rope (28) is fixedly connected between the bottom surface of the piston (30) and the inner wall of the connecting rod (9). A second elastic rope (31) is fixedly connected between the top surface of the piston (30) and the inner wall of the connecting rod (9). The elastic force of the second elastic rope (31) is greater than that of the first elastic rope (28). A limit ring (29) is fixedly connected to the inner wall of the connecting rod (9).

6. A method for detecting carbapenem resistance genes, wherein the method employs the carbapenem resistance gene detection device described in claim 5, characterized in that: The method includes the following steps: S1: By injecting carbapenem sample into microfluidic chip (6), then opening the sealing cover (2), then placing microfluidic chip (6) on limiting block (15), and then controlling the connecting plate (8) to move up and down with the help of the output end of electric telescopic rod (11) and the cooperation of the first spring, so that the connecting plate (8) pushes the gas in cavity (7) to be sprayed from the air outlet (13) onto microfluidic chip (6); S2: Press the microfluidic chip (6) to adhere to the suction cup (10), and then let the output end of the electric telescopic rod (11) move downward. At this time, the first spring will push the connecting plate (8), so that the connecting plate (8) drives the microfluidic chip (6) on the suction cup (10) to contact the inner wall of the groove (5). S3: The sample is drawn into the inlet of the microfluidic chip (6) through the pneumatic control module, flows through the chamber of pre-stored lysis reagent, and enters the heating zone after mixing. The pneumatic membrane pump drives the mixture through the built-in silica membrane micropillar or magnetic bead capture area of ​​the chip. The waste liquid is pumped into the waste liquid chamber, the purified nucleic acid is bound to the membrane / magnetic bead, and the washing buffer is pumped in for washing.

7. The method for detecting carbapenem resistance genes according to claim 6, characterized in that: The method also includes the following steps: S4: The pneumatic membrane pump accurately delivers the eluted nucleic acid solution to multiple parallel PCR micro-reaction pools of the microfluidic chip (6). At the same time, the premixed PCR master mixture is pumped in, and the nucleic acid template and reaction reagents are mixed and redissolved in the micro-reaction pool to confirm whether the liquid fills the reaction pool. S5: The cavity (7) is heated by the thermostat rod (17), and the drive blade (25) is rotated at the same time, so that the drive blade (25) stirs the gas above the connecting plate (8) until the temperature inside the cavity (7) is heated to 64℃-66℃. At this time, the heat inside the cavity (7) is applied to the microfluidic chip (6) on the groove (5) to provide a constant temperature effect for the microfluidic chip (6). S6: The optical module is responsible for exciting fluorescence signals and collecting detection data to complete the detection of carbapenem resistance genes.

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