Method for improving germination rate of polygonatum kingianum seeds

Abstract

The invention relates to the technical field of polygonatum kingianum seed treatment, in particular to a method for increasing the germination rate of polygonatum kingianum seeds, which comprises the following steps: S1, selecting polygonatum kingianum seeds; s2, the treated polygonatum kingianum seeds are subjected to low-temperature treatment, surface disinfection and moisture absorption; s3, soaking the polygonatum kingianum seeds in water at the water temperature of 25-60 DEG C for 20 minutes, cooling to room temperature after soaking, disinfecting, washing with sterile water, and absorbing moisture; s4, taking a tissue culture bottle, cleaning, drying, putting multiple layers of filter paper, sterilizing, cooling to room temperature, dropwise adding sterile water, infiltrating the filter paper in the tissue culture bottle, putting the polygonatum kingianum seeds on the filter paper in the tissue culture bottle, and covering the tissue culture bottle with a bottle cover; s5, putting the tissue culture bottle into an artificial climate box, and culturing for 12-14 days in a dark place at the temperature of 25 DEG C to obtain seedlings; according to the method, seed dormancy is effectively broken, so that seeds harvested in the season can rapidly germinate and be sown, and the seedling rate of polygonatum kingianum seeds is increased.

Description

Technical Field

[0001] The invention relates to the technical field of polygonatum cyrtonema seed processing, in particular to a method for improving the germination rate of polygonatum cyrtonema seeds. Background Art

[0002] Polygonatum kingianum (Polygonatum kingianum Coll. et Hemsl.) is a perennial herbaceous plant in the Liliaceae family (Liliaceae). It is one of the three main source plants of the medicinal material Polygonatum sibiricum listed in the 2020 edition of the Pharmacopoeia of the People's Republic of China. Its dried rhizome is used in traditional Chinese medicine, known as Polygonatum sibiricum, for its benefits of tonifying qi and nourishing yin, strengthening the spleen, moistening the lungs, and benefiting the kidneys. Due to its excellent medicinal properties and rich content of various active ingredients, Polygonatum kingianum is not only a valuable resource for the Traditional Chinese Medicine industry but also a key authentic medicinal material in Yunnan Province, known for its "medicinal and edible properties," and holds broad development prospects in the healthcare industry.

[0003] With the rapid increase in market demand, the large-scale and standardized cultivation of Polygonatum sibiricum has become the core of industrial development. However, there are significant bottlenecks in its propagation process, which seriously restricts the stable supply of seedlings and the rapid expansion of the industry. At present, production mainly relies on seed propagation, but Polygonatum sibiricum seeds have deep dormancy characteristics, and their natural germination process is extremely slow. They need to undergo outdoor stratification treatment for more than two years before germination. In addition, the germination rate is low and the seedlings are uneven, which cannot meet the needs of modern planting for efficient and mass production of seedlings.

[0004] The germination barriers of Polygonatum sibiricum seeds are mainly due to their complex comprehensive dormancy characteristics, including but not limited to: 1) Physiological dormancy: the embryo needs to complete morphological after-ripening and physiological after-ripening, and this process depends on specific temperature and humidity conditions; 2) Seed coat barriers: the seed coat may have mechanical constraints on the growth of the embryo or poor permeability to water and gas. In addition, a serious production practice problem is that the fruit of Polygonatum sibiricum is sticky, and the seeds are difficult to clean thoroughly, and residual pulp and sugar are often attached to the surface. In the warm and humid environment required for stratification germination, these organic residues can easily become a culture medium for the growth of fungi and bacteria, causing large quantities of seeds to rot, further significantly reducing the effective seedling rate.

[0005] To solve the above problems, existing technologies have proposed a variety of methods to promote germination and prevent mildew. The mainstream technical paths include: (1) Exogenous hormone treatment (such as GA3, 6-BA, etc.) breaks dormancy by regulating endogenous hormone levels. However, this method has extremely stringent requirements on hormone concentration and treatment time, and the operation requires high professionalism. A slight deviation can easily lead to drug damage or poor results, increasing production instability and operational difficulty. (2) Applying special germination agents or seed coating technology to combine fungicides, nutrients and growth regulators can effectively reduce the mildew rate and promote germination. However, this method is costly and may introduce chemical pesticide residues, which is contrary to the development concept of green agriculture. (3) Tissue culture technology can produce sterile seedlings with high efficiency, but it requires expensive equipment investment and professional technical personnel, has the highest production cost, and is difficult to promote and popularize among farmers.

[0006] In summary, while existing technologies can improve the germination of Polygonatum dahliae seeds to varying degrees, they all have significant limitations: complex procedures, long cycles, high costs, or violations of green production principles. Therefore, the industry urgently needs a seed germination treatment method that is simple to operate, cost-effective, environmentally friendly, efficient, and stable. Summary of the Invention

[0007] The invention aims to provide a method for improving the germination rate of Polygonatum cyrtonema seeds.

[0008] The scheme of the present invention is: A method for improving the germination rate of Polygonatum cyrtonema seeds comprises the following steps: S1. De-fleshing the collected mature polygonatum berries to obtain polygonatum seeds; S2. The treated Polygonatum cyrtonema seeds were treated at low temperature for 30-40 days, surface disinfected with 2% sodium hypochlorite for 10 minutes, rinsed with sterile water 5-6 times, and dried with filter paper; S3. Soak the Polygonatum dahliae seeds in water at 25-60°C for 20 minutes. After soaking, cool to room temperature and sterilize the surface with 3% hydrogen peroxide for 15 minutes. Rinse with sterile water 5-6 times and dry with filter paper. S4, take a tissue culture bottle, wash it, dry it, put it in multiple layers of filter paper, sterilize it at 121°C for 30 minutes, wait for the tissue culture bottle to cool to room temperature, add sterile water dropwise, soak the filter paper in the tissue culture bottle, place the Polygonatum dahliae seeds that have been dried in step S3 on the filter paper in the tissue culture bottle, and cover the tissue culture bottle with a bottle cap; S5. Place the tissue culture bottle in an artificial climate box at a temperature of 25° C. and culture in the dark for 12 to 14 days to obtain seedlings.

[0009] As a preferred technical solution, the fleshing treatment in step S1 is to select quartz sand with a particle size of 2 to 3 mm and mix it with the polygonatum berries and then rub it to remove the skin and flesh of the polygonatum berries to obtain polygonatum seeds; the polygonatum berries are polygonatum berries harvested in the season.

[0010] As a preferred technical solution, the low-temperature treatment in step S2 is to place the product at 4°C for 30 days.

[0011] As a preferred technical solution, in step S3, the Polygonatum cyrtonema seeds are placed in warm water at 50-60° C. and soaked for 20 minutes.

[0012] As a preferred technical solution, the multi-layer filter paper in step S4 is 3 layers of filter paper, and the drying is one of oven drying or air drying.

[0013] As a preferred technical solution, in step S5, the temperature in the artificial climate box is 25° C., and the cells are cultured in the dark for 12 days.

[0014] As a preferred technical solution, in the step S4, the filter paper in the tissue culture bottle is soaked and 25 μL of artificial synthetic mitogen filtered through a 0.22 μm filter membrane is added, wherein the artificial synthetic mitogen is GA3 with a concentration of 1 mg / mL.

[0015] Due to the adoption of the above technical solution, a method for improving the germination rate of polygonatum cyrtonema seeds comprises the following steps: S1, collecting and removing the flesh of the mature polygonatum cyrtonema berries to obtain polygonatum cyrtonema seeds; S2, treating the obtained polygonatum cyrtonema seeds at a low temperature for 30 to 40 d, surface disinfect with 2% sodium hypochlorite for 10 minutes, rinse with sterile water 5 to 6 times, and absorb the moisture with filter paper; S3, place the Yunnan polygonatum seeds in water for 20 minutes, the water temperature is 25 to 60 ° C, cool to room temperature after soaking, surface disinfect with 3% hydrogen peroxide for 15 minutes, rinse with sterile water 5 to 6 times, and absorb the moisture with filter paper; S4, take a tissue culture bottle, wash, dry, put in multiple layers of filter paper, sterilize at 121 ° C for 30 minutes, wait for the tissue culture bottle to cool to room temperature, add sterile water dropwise, soak the filter paper in the tissue culture bottle, place the Yunnan polygonatum seeds that have been dried in step S3 on the filter paper in the tissue culture bottle, and cover the tissue culture bottle; S5, place the tissue culture bottle in an artificial climate box at a temperature of 25 ° C, culture in the dark for 12 to 14 days to obtain seedlings.

[0016] Advantages of the present invention: The present invention overcomes the shortcomings of the existing technology and provides a new Yunnan polygonatum seed processing strategy. This strategy aims to efficiently break seed dormancy, so that seeds can be quickly germinated and sown when harvested in the same season, significantly shortening the seedling raising cycle; at the same time, the strategy must be able to effectively inhibit microbial contamination during the stratification process and significantly reduce the seed mildew rate. Thus, without relying on expensive equipment and difficult operations, the germination uniformity and final seedling rate of Yunnan polygonatum seeds are comprehensively improved, providing a reliable and practical key technical support for the large-scale seedling raising of Yunnan polygonatum. The seedling rate of Yunnan polygonatum seeds is significantly improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0018] Figure 1 This is a picture of the Polygonatum sibiricum berries used in the experiments of the present invention; Figure 2 This is a diagram of the Polygonatum dahliae seeds used in the experiments of the present invention; Figure 3 This is a comparison chart of the germination of Polygonatum cyrtonema seeds in the experiment of the present invention, wherein three adjacent Polygonatum cyrtonema seeds are a group, from left to right are comparison group 1, experimental group 1, experimental group 2, experimental group 3, experimental group 4, and comparison group 2. DETAILED DESCRIPTION

[0019] In order to make up for the above deficiencies, the present invention provides a method for improving the germination rate of Polygonatum cyrtonema seeds to solve the problems in the above background technology.

[0020] In order to make the technical means, creative features, objectives and effects achieved by the present invention easier to understand, the present invention is further described below in conjunction with specific embodiments.

[0021] Example 1 S1. De-fleshing the collected mature polygonatum berries to obtain polygonatum seeds; S2. The treated Polygonatum cyrtonema seeds were treated at low temperature for 30 days, surface disinfected with 2% sodium hypochlorite for 10 minutes, rinsed with sterile water 5-6 times, and dried with filter paper; S3. Soak the seeds of Polygonatum dahliae in water for 20 minutes at 25°C (room temperature). Remove and surface-sterilize them with 3% hydrogen peroxide for 15 minutes. Rinse with sterile water 5-6 times and dry with filter paper. S4. Take a tissue culture bottle, wash it, dry it, put it in multiple layers of filter paper, and sterilize it at 121°C for 30 min. After the tissue culture bottle cools to room temperature, add sterile water dropwise to soak the filter paper in the tissue culture bottle, and add 25 μL of 6-BA (concentration of 1 mg / mL) filtered through a 0.22 μm filter membrane. Place the Polygonatum dahliae seeds that have been dried in step S3 on the filter paper in the tissue culture bottle, and cover the tissue culture bottle with a cap. S5. Place the tissue culture bottle in an artificial climate box at 25° C. and culture in the dark for 14 days to obtain seedlings.

[0022] In the step S1, the fleshing process is to select quartz sand with a particle size of 2 to 3 mm and mix it with the polygonatum berries, and then knead them to remove the skin and flesh of the polygonatum berries to obtain polygonatum seeds.

[0023] The low temperature treatment in step S2 is 4°C.

[0024] Example 2 S1. De-fleshing the collected mature polygonatum berries to obtain polygonatum seeds; S2. The treated Polygonatum cyrtonema seeds were treated at low temperature for 30 days, surface disinfected with 2% sodium hypochlorite for 10 minutes, rinsed with sterile water 5-6 times, and dried with filter paper; S3. Soak the seeds of Polygonatum dahliae in water for 20 minutes at 25°C (room temperature). Remove and surface-sterilize them with 3% hydrogen peroxide for 15 minutes. Rinse with sterile water 5-6 times and dry with filter paper. S4. Take a tissue culture bottle, wash it, dry it, put it in multiple layers of filter paper, and sterilize it at 121°C for 30 minutes. After the tissue culture bottle cools to room temperature, add sterile water dropwise to soak the filter paper in the tissue culture bottle, and add 25 μL of GA3 (concentration of 1 mg / mL) filtered through a 0.22 μm filter membrane. Place the Polygonatum dahliae seeds that have been dried in step S3 on the filter paper in the tissue culture bottle, and cover the tissue culture bottle with a cap. S5. Place the tissue culture bottle into an artificial climate box at 25° C. and culture in the dark for 14 days to obtain seedlings.

[0025] In the step S1, the fleshing process is to select quartz sand with a particle size of 2 to 3 mm and mix it with the polygonatum berries, then knead them to remove the skin and flesh of the polygonatum berries to obtain polygonatum seeds; the polygonatum berries are polygonatum berries harvested in the season.

[0026] As a preferred technical solution, the low temperature treatment in step S2 is 4°C.

[0027] Example 3 S1. De-fleshing the collected mature polygonatum berries to obtain polygonatum seeds; S2. The treated Polygonatum cyrtonema seeds were treated at low temperature for 30 days, surface disinfected with 2% sodium hypochlorite for 10 minutes, rinsed with sterile water 5-6 times, and dried with filter paper; S3. Soak the Polygonatum dahliae seeds in water at 50°C for 20 minutes. After soaking, cool to room temperature and sterilize the surface with 3% hydrogen peroxide for 15 minutes. Rinse with sterile water 5-6 times and dry with filter paper. S4, take a tissue culture bottle, wash it, dry it, put it in multiple layers of filter paper, sterilize it at 121°C for 30 minutes, wait for the tissue culture bottle to cool to room temperature, add sterile water dropwise, soak the filter paper in the tissue culture bottle, place the Polygonatum dahliae seeds that have been dried in step S3 on the filter paper in the tissue culture bottle, and cover the tissue culture bottle with a bottle cap; S5. Place the tissue culture bottle in an artificial climate box at 25° C. and culture in the dark for 12 days to obtain seedlings.

[0028] In the step S1, the fleshing process is to select quartz sand with a particle size of 2 to 3 mm and mix it with the polygonatum berries, then knead them to remove the skin and flesh of the polygonatum berries to obtain polygonatum seeds; the polygonatum berries are polygonatum berries harvested in the season.

[0029] The low temperature treatment in step S2 is 4°C.

[0030] In the step S4, the multi-layer filter paper is 3 layers of filter paper, and the drying is air-drying.

[0031] Example 4 S1. De-fleshing the collected mature polygonatum berries to obtain polygonatum seeds; S2. The treated Polygonatum cyrtonema seeds were treated at low temperature for 30 days, surface disinfected with 2% sodium hypochlorite for 10 minutes, rinsed with sterile water 5-6 times, and dried with filter paper; S3. Soak the Polygonatum dahliae seeds in water at 60°C for 20 minutes. After soaking, cool to room temperature and sterilize the surface with 3% hydrogen peroxide for 15 minutes. Rinse with sterile water 5-6 times and dry with filter paper. S4, take a tissue culture bottle, wash it, dry it, put it in multiple layers of filter paper, sterilize it at 121°C for 30 minutes, wait for the tissue culture bottle to cool to room temperature, add sterile water dropwise, soak the filter paper in the tissue culture bottle, place the Polygonatum dahliae seeds that have been dried in step S3 on the filter paper in the tissue culture bottle, and cover the tissue culture bottle with a bottle cap; S5. Place the tissue culture bottle in an artificial climate box at 25° C. and culture in the dark for 12 days to obtain seedlings.

[0032] In the step S1, the fleshing process is to select quartz sand with a particle size of 2 to 3 mm and mix it with the polygonatum berries, then knead them to remove the skin and flesh of the polygonatum berries to obtain polygonatum seeds; the polygonatum berries are polygonatum berries harvested in the season.

[0033] The low temperature treatment in step S2 is 4°C.

[0034] In the step S4, the multi-layer filter paper is 3 layers of filter paper, and the drying is air-drying.

[0035] Example 5 S1. De-fleshing the collected mature polygonatum berries to obtain polygonatum seeds; S2. The treated Polygonatum cyrtonema seeds were treated at low temperature for 40 days, surface disinfected with 2% sodium hypochlorite for 10 minutes, rinsed with sterile water 5-6 times, and dried with filter paper; S3. Soak the Polygonatum dahliae seeds in water at 54°C for 20 minutes. After soaking, cool to room temperature and sterilize the surface with 3% hydrogen peroxide for 15 minutes. Rinse with sterile water 5-6 times and dry with filter paper. S4, take a tissue culture bottle, wash it, dry it, put it in multiple layers of filter paper, sterilize it at 121°C for 30 minutes, wait for the tissue culture bottle to cool to room temperature, add sterile water dropwise, soak the filter paper in the tissue culture bottle, place the Polygonatum dahliae seeds that have been dried in step S3 on the filter paper in the tissue culture bottle, and cover the tissue culture bottle with a bottle cap; S5. Place the tissue culture bottle into an artificial climate box at a temperature of 25° C. and culture in the dark for 13 days to obtain seedlings.

[0036] In step S1, the fleshing process is to select quartz sand with a particle size of 2 to 3 mm and mix it with the polygonatum berries, then knead them to remove the skin and flesh of the polygonatum berries to obtain polygonatum seeds; the polygonatum berries are polygonatum berries harvested in season.

[0037] The low temperature treatment in step S2 is to place the sample at 4° C. for 40 days.

[0038] In the step S4, the multi-layer filter paper is 3 layers of filter paper, and the drying is oven drying.

[0039] Example 6 S1. De-fleshing the collected mature polygonatum berries to obtain polygonatum seeds; S2. The treated Polygonatum cyrtonema seeds were treated at low temperature for 35 days, surface disinfected with 2% sodium hypochlorite for 10 minutes, rinsed with sterile water 5-6 times, and dried with filter paper; S3. Soak the Polygonatum dahliae seeds in water at 58°C for 20 minutes. After soaking, cool to room temperature and sterilize the surface with 3% hydrogen peroxide for 15 minutes. Rinse with sterile water 5-6 times and dry with filter paper. S4, take a tissue culture bottle, wash it, dry it, put it in multiple layers of filter paper, sterilize it at 121°C for 30 minutes, wait for the tissue culture bottle to cool to room temperature, add sterile water dropwise, soak the filter paper in the tissue culture bottle, place the Polygonatum dahliae seeds that have been dried in step S3 on the filter paper in the tissue culture bottle, and cover the tissue culture bottle with a bottle cap; S5. Place the tissue culture bottle in an artificial climate box at 25° C. and culture in the dark for 14 days to obtain seedlings.

[0040] In the step S1, the fleshing process is to select quartz sand with a particle size of 2 to 3 mm and mix it with the polygonatum berries, then knead them to remove the skin and flesh of the polygonatum berries to obtain polygonatum seeds; the polygonatum berries are polygonatum berries harvested in the season.

[0041] In the step S2, the low temperature treatment is performed at 4° C. for 35 days.

[0042] As a preferred technical solution, in step S3, the Polygonatum cyrtonema seeds are placed in warm water at 58° C. and soaked for 20 minutes.

[0043] In the step S4, the multi-layer filter paper is 3 layers of filter paper, and the drying is oven drying.

[0044] Comparison group 1 S1. De-fleshing the collected mature polygonatum berries to obtain polygonatum seeds; S2. The treated Polygonatum cyrtonema seeds were treated at low temperature for 30 days, surface disinfected with 2% sodium hypochlorite for 10 minutes, rinsed with sterile water 5-6 times, and dried with filter paper; S3. Soak the seeds of Polygonatum dahliae in water for 20 minutes at 25°C (room temperature). Remove and surface-sterilize them with 3% hydrogen peroxide for 15 minutes. Rinse with sterile water 5-6 times and dry with filter paper. S4, take a tissue culture bottle, wash it, dry it, put it in multiple layers of filter paper, sterilize it at 121°C for 30 minutes, wait for the tissue culture bottle to cool to room temperature, add sterile water dropwise, soak the filter paper in the tissue culture bottle, place the Polygonatum dahliae seeds that have been dried in step S3 on the filter paper in the tissue culture bottle, and cover the tissue culture bottle with a bottle cap; S5. Place the tissue culture bottle in an artificial climate box at 25° C. and culture in the dark for 14 days to obtain seedlings.

[0045] In the step S1, the fleshing process is to select quartz sand with a particle size of 2 to 3 mm and mix it with the polygonatum berries, and then knead them to remove the skin and flesh of the polygonatum berries to obtain polygonatum seeds.

[0046] The low temperature treatment in step S2 is 4°C.

[0047] Comparison group 2 S1. De-fleshing the collected mature polygonatum berries to obtain polygonatum seeds; S2. The treated Polygonatum cyrtonema seeds were treated at low temperature for 40 days, surface disinfected with 2% sodium hypochlorite for 10 minutes, rinsed with sterile water 5-6 times, and dried with filter paper; S3. Soak the seeds of Polygonatum dahliae in water for 20 minutes at 25°C (room temperature). Remove and surface-sterilize them with 3% hydrogen peroxide for 15 minutes. Rinse with sterile water 5-6 times and dry with filter paper. S4, take a tissue culture bottle, wash it, dry it, put it in multiple layers of filter paper, sterilize it at 121°C for 30 minutes, wait for the tissue culture bottle to cool to room temperature, add sterile water dropwise, soak the filter paper in the tissue culture bottle, place the Polygonatum dahliae seeds that have been dried in step S3 on the filter paper in the tissue culture bottle, and cover the tissue culture bottle with a bottle cap; S5. Place the tissue culture bottle in an artificial climate box at 25° C. and culture in the dark for 14 days to obtain seedlings.

[0048] In the step S1, the fleshing process is to select quartz sand with a particle size of 2 to 3 mm and mix it with the polygonatum berries, and then knead them to remove the skin and flesh of the polygonatum berries to obtain polygonatum seeds.

[0049] The low temperature treatment in step S2 is 4°C.

[0050] experiment: The germination rate of Example 1 group (experimental group 3), Example 2 group (experimental group 4), Example 3 group (ie, experimental group 1), Example 4 group (ie, experimental group 2), comparison group 1, and comparison group 2 were tested. Figure 1 As shown in the figure, the treated Polygonatum dahliae seeds are as follows Figure 2 As shown, the method of control group 1 was the same as that of the example group, except that the soaking water temperature in step S3 was 25°C and no hormones were added in step S4. Six groups were selected from each of the six groups, for a total of 360 seeds. The germination status was recorded at 3d, 5d, 7d, 10d, and 12d after germination, with the radicle breaking through the seed coat as the germination sign.

[0051] Seed germination rate (%) = (final number of germinated seeds / number of test seeds) × 100%; Table 1 Germination rate under different seed soaking treatments Table 1 shows the germination rates under different low temperature treatment days, different seed soaking and hormone treatments. On the 3rd day, the seeds in the seed soaking treatment began to germinate, and the germination rates of experimental groups 1 and 2 were 25% and 22%, respectively. On the 5th day, the hormone treatment began to germinate, and the germination rates of experimental groups 3 and 4 were 5% and 22%, respectively. On the 7th day, the control group began to germinate, and the germination rates of control group 2, control group 1, experimental group 1, experimental group 2, experimental group 3, and experimental group 4 were 2%, 3%, 80%, 92%, 60%, and 48%, respectively. By the 12th day, the germination rates of the six groups were 8%, 12%, 95%, 98%, 77%, and 78%, respectively. The length of the buds was 5 mm, 8 mm, 47 mm, 32 mm, 15 mm, and 20 mm ( Figure 3 The results showed that soaking in warm water at 50-60℃ could increase the germination rate of Polygonatum cyrtonema seeds.

[0052] The basic principles, main features, and advantages of the present invention are shown and described above. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and modifications may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and modifications are intended to fall within the scope of the present invention. The scope of protection claimed in the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for improving the germination rate of Polygonatum cyrtonema seeds, characterized in that: The following steps are involved: S1. De-fleshing the collected mature polygonatum berries to obtain polygonatum seeds; S2. The treated Polygonatum cyrtonema seeds were treated at low temperature for 30-40 days, surface disinfected with 2% sodium hypochlorite for 10 minutes, rinsed with sterile water 5-6 times, and dried with filter paper; S3. Soak the Polygonatum dahliae seeds in water at 25-60°C for 20 minutes. After soaking, cool to room temperature and sterilize the surface with 3% hydrogen peroxide for 15 minutes. Rinse with sterile water 5-6 times and dry with filter paper. S4, take a tissue culture bottle, wash it, dry it, put it in multiple layers of filter paper, sterilize it at 121°C for 30 minutes, wait for the tissue culture bottle to cool to room temperature, add sterile water dropwise, soak the filter paper in the tissue culture bottle, place the Polygonatum dahliae seeds that have been dried in step S3 on the filter paper in the tissue culture bottle, and cover the tissue culture bottle with a bottle cap; S5. Place the tissue culture bottle in an artificial climate box at a temperature of 25° C. and culture in the dark for 12 to 14 days to obtain seedlings.

2. The method for improving the germination rate of Polygonatum cyrtonema seeds according to claim 1, wherein: In the step S1, the fleshing process is to select quartz sand with a particle size of 2 to 3 mm and mix it with the polygonatum berries, then knead them to remove the skin and flesh of the polygonatum berries to obtain polygonatum seeds; the polygonatum berries are polygonatum berries harvested in the season.

3. The method for improving the germination rate of Polygonatum cyrtonema seeds according to claim 1, wherein: The low temperature treatment in step S2 is performed at 4°C for 30 days.

4. The method for improving the germination rate of Polygonatum cyrtonema seeds according to claim 1, wherein: In the step S3, the Polygonatum cyrtonema seeds are placed in warm water at 50-60° C. and soaked for 20 minutes.

5. The method for improving the germination rate of Polygonatum cyrtonema seeds according to claim 1, wherein: In the step S4, the multi-layer filter paper is a three-layer filter paper, and the drying is one of oven drying or air drying.

6. The method for improving the germination rate of Polygonatum cyrtonema seeds according to claim 1, wherein: In the step S5, the temperature in the artificial climate box is 25° C. and the cells are cultured in the dark for 12 days.

7. The method for improving the germination rate of Polygonatum cyrtonema seeds according to claim 1, wherein: In the step S4, the filter paper in the tissue culture bottle is soaked, and 25 μL of artificial synthetic mitogen filtered through a 0.22 μm filter membrane is added. The artificial synthetic mitogen is GA3 with a concentration of 1 mg / mL.

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