Special fermented feed for rhizoma anemones raddeanae of rumex hanus and preparation method thereof

Abstract

The invention relates to an animal husbandry feed technology, in particular to a special fermented feed for azolla filliculoidas and a preparation method thereof, and the special fermented feed for azolla filliculoidas is prepared from azolla filliculoidas, a functional composition, corn flour, soybean meal, bran, molasses, a compound fermentation inoculant, dicalcium phosphate, mountain flour, table salt, compound trace elements and compound vitamins; wherein the functional composition comprises puffed edible amaranth seeds, bacterial cellulose, tannase, phytase, zein and phospholipid; a three-in-one enzyme targeting delivery system comprising expanded carrier skeleton, bacterial cellulose dispersion liquid reinforcement, protein phospholipid coating and the like is adopted, and a compound fermentation inoculant is combined for synergistic fermentation, so that accurate degradation of anti-nutritional factors in intestinal tracts, efficient utilization of high-tannin raw materials and full release of the growth potential of the rhizoma anemones raddeanae are realized.

Description

Technical Field

[0001] The invention relates to animal husbandry feed technology, in particular to a special fermented feed for the leaf-eating herb Polygonum multiflorum and a preparation method thereof. Background Art

[0002] Leaf-eating grass is a plant of the genus Rumex in the Polygonaceae family, also known as leaf-eating grass sorrel, leaf-eating vegetable, and protein grass. It is obtained by hybridizing Rumex K-1 as the female parent and Rumex barthinifolia as the male parent. The plant is tall, with a thick main root, an upright stem, clustered leaves, smooth and entire margins, and green leaves. Leaf-eating grass is a salt-alkali resistant crop with low soil requirements. It can be grown in both southern and northern China. It has the characteristics of salt-alkali tolerance, fast growth, and high yield. Because leaf-eating grass is a herbaceous plant, its nutrition is higher than that of vegetables, and it has good palatability, making it suitable for the development of vegetables, food, medicine, feed, etc.

[0003] The Chinese wolf is native to Jinhua, Dongyang, Yiwu and other places in Zhejiang Province, China. Its distinctive feature is that the hair on the head, neck and rump is black, and the rest of the hair is white. Therefore, it is also called "Chinese wolf". For example, Jinhua Chinese wolf has excellent properties such as early maturity, good meat quality and high reproduction rate. It is the core raw material for making traditional famous products such as Jinhua ham.

[0004] However, foliage grass contains anti-nutritional factors, such as tannins and phytic acid, which result in poor nutrient absorption. In view of this, we propose a fermented feed specifically for foliage grass Polygonum multiflorum and a preparation method thereof. Summary of the Invention

[0005] The purpose of the present invention is to provide a special fermented feed for the leaf-eating herb Polygonum multiflorum and a preparation method thereof, so as to solve the problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides a special fermented feed for leaf-eating grass and Chinese wolfberry, which comprises leaf-eating grass, a functional composition, corn flour, soybean meal, bran, molasses, a composite fermentation agent, calcium hydrogen phosphate, stone powder, salt, composite trace elements, and composite vitamins;

[0007] The functional composition comprises puffed amaranth seeds, bacterial cellulose, tannase, phytase, zein and lecithin.

[0008] Preferably, the edible grass comprises 25-35 parts by weight, the functional composition comprises 3-8 parts by weight, corn flour comprises 30-40 parts by weight, soybean meal comprises 12-18 parts by weight, bran comprises 5-10 parts by weight, molasses comprises 2-5 parts by weight, composite fermentation agent comprises 0.1-0.3 parts by weight, calcium hydrogen phosphate comprises 0.5-1.2 parts by weight, stone powder comprises 0.3-0.8 parts by weight, salt comprises 0.2-0.5 parts by weight, composite trace elements comprises 0.3-0.6 parts by weight, and composite vitamins comprises 0.4-0.8 parts by weight.

[0009] Preferably, the composite fermentation agent contains 1-5×10 Lactobacillus plantarum 10 CFU / g, Saccharomyces cerevisiae 0.5-2×10 9 CFU / g, Bacillus subtilis 1-3×10 9 CFU / g.

[0010] Preferably, the complex trace elements include Fe, Zn, Cu, Mn, I, Se, and Co, and the complex vitamins include A, D3, E, K3, and B group.

[0011] Preferably, the preparation method of the functional composition is as follows:

[0012] S1.1. Prepare puffed amaranth seeds in a hot press puffing machine at a temperature of 150-180°C, a pressure of 0.6-0.8 MPa, and puffing for 15-30 seconds;

[0013] S1.2. First, bacterial cellulose is mixed with deionized water to prepare a bacterial cellulose dispersion. The puffed amaranth seed particles and the bacterial cellulose dispersion are uniformly mixed at a solid-to-liquid ratio of 1:2 to 5 g / mL, wherein the dry weight of the bacterial cellulose is 4-8% of the dry weight of the puffed amaranth seeds. The mixture is freeze-dried to obtain fortified amaranth seeds.

[0014] S1.3, dissolving tannase and phytase in a 0.1 M acetic acid buffer solution with a pH of 4.8-5.2 at a mixing mass ratio of 1:0.5-1 to obtain an enzyme solution;

[0015] S1.4. Immerse the fortified amaranth seed carrier in the enzyme solution, evacuate to -0.08 MPa, maintain for 5 min, and pulse pressurize to 0.2 MPa at a frequency of 0.5 Hz for 3 cycles to obtain the enzyme carrier.

[0016] S1.5. Add the enzyme carrier to a 5-15% w / v zein-phospholipid ethanol solution and coat in a fluidized bed at an inlet air temperature of 35-40°C, an atomizing pressure of 0.8 bar, a spray rate of 2 mL / min, and a fluidizing air volume of 20 m 3 / h, the thickness of the coating layer is 10-30 μm, and a functional composition is obtained.

[0017] Amaranth seeds are processed to form a high specific surface area and porous "popcorn" structure with a burnt aroma, which can enhance the flavor and mask the grassy smell of foliage grass, and load tannins and phytase. When used, due to its high specific surface area and porous structure after expansion, it adsorbs tannins and phytase, and adds bacterial cellulose dispersion as support, and is coated with alcohol-soluble protein to form a capsule structure to protect tannins, shield the enzyme from damage by extreme conditions such as gastric acid, bile, high temperature and high humidity, prevent it from being inactivated prematurely, and release its activity in the intestine, thereby achieving the purpose of reducing anti-nutritional factors and promoting the growth and development of Amaranth.

[0018] On the other hand, the present invention provides a method for preparing a fermented feed specifically for the leaf-eating herb Ampelopsis pilosula, which is used for any of the above-mentioned fermented feed specifically for the leaf-eating herb Ampelopsis pilosula, comprising the following steps:

[0019] S2.1. Crush the leaf-eating grass, spray it with 0.5-0.7% w / w cellulase solution, and let it stand at 40-45° C. for 5-6 hours to obtain pretreated leaf-eating grass;

[0020] S2.2. Mix corn flour, soybean meal, bran, calcium hydrogen phosphate, stone powder, salt, complex trace elements, and complex vitamins in a twin-shaft paddle mixer, add edible grass, molasses, and the functional composition, and stir evenly;

[0021] S2.3. Add 35℃ warm water into the composite fermentation agent at a ratio of 1:10, let it stand for 15 minutes for activation, spray the activated bacterial solution evenly into the mixture, continue stirring for 3 minutes, and then fill it into the fermentation tank at a temperature of 35±2℃ and a water content of 40-42% for 48-72 hours. Stop the fermentation when the pH value drops to 4.2-4.5, and transfer it to a 4℃ cold storage for granulation. The particle size is 3-4mm to obtain a special fermented feed for leaf-eating herbaceous blackbird.

[0022] Preferably, the cellulase is a composite enzyme system of endo-β-glucanase and exo-β-glucanase, with an enzyme activity ratio of 1:0.8-1.2.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] The invention relates to a special fermented feed for leaf-eating amaranth, and a preparation method thereof. The feed comprises puffed amaranth seeds as a carrier, bacterial cellulose dispersion for reinforcement, a dual-enzyme system, and zein and phospholipid coating. The high specific surface area and porous structure of the puffed amaranth seeds are utilized to facilitate the adsorption of tannase and phytase. The mechanical strength of the bacterial cellulose dispersion is used to strengthen the structure of the puffed amaranth seeds. The zein and phospholipid coatings are used to prevent the external environment from damaging the enzyme activity, thereby achieving efficient targeted removal of anti-nutritional factors, improving feed conversion rate, and significantly improving the growth performance of the amaranth. DETAILED DESCRIPTION

[0025] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0026] The invention discloses a special fermented feed for leaf-eating grass-bearing Chinese wolfberry, which comprises leaf-eating grass, a functional composition, corn flour, soybean meal, bran, molasses, a composite fermentation agent, calcium hydrogen phosphate, stone powder, salt, composite trace elements, and composite vitamins.

[0027] The functional composition comprises puffed amaranth seeds, bacterial cellulose, tannase, phytase, zein and lecithin;

[0028] Complex trace elements include Fe, Zn, Cu, Mn, I, Se, Co, and complex vitamins include A, D3, E, K3, and B group;

[0029] Bacterial cellulose is composed of glucose chains secreted by a microorganism (Gluconacetobacter) that forms a nanoscale fiber network.

[0030] Example 1: A fermented feed specifically for leaf-eating herb Polygonum multiflorum and a preparation method thereof, comprising the following steps:

[0031] Prepare the following components: 35 parts by weight of edible grass, 3 parts by weight of the functional composition, 40 parts by weight of corn flour, 18 parts by weight of soybean meal, 10 parts by weight of bran, 5 parts by weight of molasses, 0.3 parts by weight of a composite fermentation agent, 1.2 parts by weight of calcium hydrogen phosphate, 0.8 parts by weight of stone powder, 0.5 parts by weight of salt, 0.6 parts by weight of a composite trace element, and 0.8 parts by weight of a composite vitamin.

[0032] The functional composition includes puffed amaranth seeds, bacterial cellulose, tannin, phytase, zein, and phospholipids; the tannin activity is 500 U / g, the phytase activity is 800 U / g; the mass ratio of zein to phospholipids is 10:4, and the ethanol concentration is 80% v / v; the ratio of the fortified amaranth seed carrier to the enzyme solution is 1 g:10 mL; the composite fermentation agent contains 5×10 Lactobacillus plantarum 10 CFU / g, Saccharomyces cerevisiae 2×10 9 CFU / g, Bacillus subtilis 3×10 9 CFU / g; Cellulase is a composite enzyme system of endo-β-glucanase and exo-β-glucanase, with an enzyme activity ratio of 1:1.2;

[0033] S1.1. Prepare puffed amaranth seeds in a hot press puffing machine at a temperature of 150°C and a pressure of 0.8 MPa for 30 seconds.

[0034] S1.2. First, bacterial cellulose is mixed with deionized water to prepare a bacterial cellulose dispersion. The puffed amaranth seed particles and the bacterial cellulose dispersion are mixed evenly at a solid-liquid ratio of 1:5 g / mL, wherein the dry weight of the bacterial cellulose accounts for 4% of the dry weight of the puffed amaranth seeds. The mixture is freeze-dried to obtain fortified amaranth seeds.

[0035] S1.3, dissolving tannase and phytase in a 0.1 M, pH 5.2 acetate buffer at a mixing ratio of 1:1 to obtain an enzyme solution;

[0036] S1.4. Immerse the fortified amaranth seed carrier in the enzyme solution, evacuate to -0.08 MPa, maintain for 5 min, and pulse pressurize to 0.2 MPa at a frequency of 0.5 Hz for 3 cycles to obtain the enzyme carrier.

[0037] S1.5. Add the enzyme carrier to a 15% w / v zein-phospholipid ethanol solution and coat in a fluidized bed with an inlet air temperature of 35°C, an atomizing pressure of 0.8 bar, a spray rate of 2 mL / min, and a fluidizing air volume of 20 m 3 / h, the thickness of the coating layer is 30 μm, and a functional composition is obtained;

[0038] S2.1. Crush the leaf-eating grass, spray it with 0.5% w / w cellulase solution, and let it stand at 45°C for 6 hours to obtain pretreated leaf-eating grass;

[0039] S2.2. Mix corn flour, soybean meal, bran, calcium hydrogen phosphate, stone powder, salt, complex trace elements, and complex vitamins in a twin-shaft paddle mixer, add edible grass, molasses, and the functional composition, and stir evenly;

[0040] S2.3. Add 35℃ warm water into the composite fermentation agent at a ratio of 1:10, let it stand for 15 minutes for activation, spray the activated bacterial solution evenly into the mixture, continue stirring for 3 minutes, and then fill it into the fermentation tank at a temperature of 35℃, a water content of 42%, and a time of 72 hours. Stop the fermentation when the pH value drops to 4.2, transfer it to a 4℃ cold storage for granulation, and the particle size is 3mm to obtain a special fermented feed for leaf-eating herbaceous mulberry.

[0041] Example 2: A fermented feed specifically for leaf-eating herb Polygonum multiflorum and a preparation method thereof, comprising the following steps:

[0042] Prepare the following components: 35 parts by weight of edible grass, 5.5 parts by weight of the functional composition, 40 parts by weight of corn flour, 18 parts by weight of soybean meal, 10 parts by weight of bran, 5 parts by weight of molasses, 0.3 parts by weight of a composite fermentation agent, 1.2 parts by weight of calcium hydrogen phosphate, 0.8 parts by weight of stone powder, 0.5 parts by weight of salt, 0.6 parts by weight of a composite trace element, and 0.8 parts by weight of a composite vitamin.

[0043] The functional composition includes puffed amaranth seeds, bacterial cellulose, tannin, phytase, zein, and phospholipids; the tannin activity is 500 U / g, the phytase activity is 800 U / g; the mass ratio of zein to phospholipids is 10:4, and the ethanol concentration is 80% v / v; the ratio of the fortified amaranth seed carrier to the enzyme solution is 1 g:10 mL; the composite fermentation agent contains 5×10 Lactobacillus plantarum 10 CFU / g, Saccharomyces cerevisiae 2×10 9 CFU / g, Bacillus subtilis 3×10 9 CFU / g; Cellulase is a composite enzyme system of endo-β-glucanase and exo-β-glucanase, with an enzyme activity ratio of 1:1.2;

[0044] S1.1. Prepare puffed amaranth seeds in a hot press puffing machine at a temperature of 150°C and a pressure of 0.8 MPa for 30 seconds.

[0045] S1.2. First, bacterial cellulose is mixed with deionized water to prepare a bacterial cellulose dispersion. The puffed amaranth seed particles and the bacterial cellulose dispersion are mixed evenly at a solid-liquid ratio of 1:5 g / mL, wherein the dry weight of the bacterial cellulose accounts for 4% of the dry weight of the puffed amaranth seeds. The mixture is freeze-dried to obtain fortified amaranth seeds.

[0046] S1.3, dissolving tannase and phytase in a 0.1 M, pH 5.2 acetate buffer at a mixing ratio of 1:1 to obtain an enzyme solution;

[0047] S1.4. Immerse the fortified amaranth seed carrier in the enzyme solution, evacuate to -0.08 MPa, maintain for 5 min, and pulse pressurize to 0.2 MPa at a frequency of 0.5 Hz for 3 cycles to obtain the enzyme carrier.

[0048] S1.5. Add the enzyme carrier to a 15% w / v zein-phospholipid ethanol solution and coat the enzyme in a fluidized bed at an inlet air temperature of 35°C, an atomization pressure of 0.8 bar, a spray rate of 2 mL / min, a fluidizing air volume of 20 m3 / h, and a coating layer thickness of 30 μm to obtain a functional composition;

[0049] S2.1. Crush the leaf-eating grass, spray it with 0.5% w / w cellulase solution, and let it stand at 45°C for 6 hours to obtain pretreated leaf-eating grass;

[0050] S2.2. Mix corn flour, soybean meal, bran, calcium hydrogen phosphate, stone powder, salt, complex trace elements, and complex vitamins in a twin-shaft paddle mixer, add edible grass, molasses, and the functional composition, and stir evenly;

[0051] S2.3. Add 35℃ warm water into the composite fermentation agent at a ratio of 1:10, let it stand for 15 minutes for activation, spray the activated bacterial solution evenly into the mixture, continue stirring for 3 minutes, and then fill it into the fermentation tank at a temperature of 35℃, a water content of 42%, and a time of 72 hours. Stop the fermentation when the pH value drops to 4.2, transfer it to a 4℃ cold storage for granulation, and the particle size is 3mm to obtain a special fermented feed for leaf-eating herbaceous mulberry.

[0052] Example 3: A fermented feed specifically for leaf-eating herb Polygonum multiflorum and a preparation method thereof, comprising the following steps:

[0053] Prepare the following components: 35 parts by weight of edible grass, 8 parts by weight of the functional composition, 40 parts by weight of corn flour, 18 parts by weight of soybean meal, 10 parts by weight of bran, 5 parts by weight of molasses, 0.3 parts by weight of a composite fermentation agent, 1.2 parts by weight of calcium hydrogen phosphate, 0.8 parts by weight of stone powder, 0.5 parts by weight of salt, 0.6 parts by weight of a composite trace element, and 0.8 parts by weight of a composite vitamin.

[0054] The functional composition includes puffed amaranth seeds, bacterial cellulose, tannin, phytase, zein, and phospholipids; the tannin activity is 500 U / g, the phytase activity is 800 U / g; the mass ratio of zein to phospholipids is 10:4, and the ethanol concentration is 80% v / v; the ratio of the fortified amaranth seed carrier to the enzyme solution is 1 g:10 mL; the composite fermentation agent contains 5×10 Lactobacillus plantarum 10 CFU / g, Saccharomyces cerevisiae 2×10 9 CFU / g, Bacillus subtilis 3×10 9 CFU / g; Cellulase is a composite enzyme system of endo-β-glucanase and exo-β-glucanase, with an enzyme activity ratio of 1:1.2;

[0055] S1.1. Prepare puffed amaranth seeds in a hot press puffing machine at a temperature of 150°C and a pressure of 0.8 MPa for 30 seconds.

[0056] S1.2. First, bacterial cellulose is mixed with deionized water to prepare a bacterial cellulose dispersion. The puffed amaranth seed particles and the bacterial cellulose dispersion are mixed evenly at a solid-liquid ratio of 1:5 g / mL, wherein the dry weight of the bacterial cellulose accounts for 4% of the dry weight of the puffed amaranth seeds. The mixture is freeze-dried to obtain fortified amaranth seeds.

[0057] S1.3, dissolving tannase and phytase in a 0.1 M, pH 5.2 acetate buffer at a mixing ratio of 1:1 to obtain an enzyme solution;

[0058] S1.4. Immerse the fortified amaranth seed carrier in the enzyme solution, evacuate to -0.08 MPa, maintain for 5 min, and pulse pressurize to 0.2 MPa at a frequency of 0.5 Hz for 3 cycles to obtain the enzyme carrier.

[0059] S1.5. Add the enzyme carrier to a 15% w / v zein-phospholipid ethanol solution and coat in a fluidized bed with an inlet air temperature of 35°C, an atomizing pressure of 0.8 bar, a spray rate of 2 mL / min, and a fluidizing air volume of 20 m 3 / h, the thickness of the coating layer is 30 μm, and a functional composition is obtained;

[0060] S2.1. Crush the leaf-eating grass, spray it with 0.5% w / w cellulase solution, and let it stand at 45°C for 6 hours to obtain pretreated leaf-eating grass;

[0061] S2.2. Mix corn flour, soybean meal, bran, calcium hydrogen phosphate, stone powder, salt, complex trace elements, and complex vitamins in a twin-shaft paddle mixer, add edible grass, molasses, and the functional composition, and stir evenly;

[0062] S2.3. Add 35℃ warm water into the composite fermentation agent at a ratio of 1:10, let it stand for 15 minutes for activation, spray the activated bacterial solution evenly into the mixture, continue stirring for 3 minutes, and then fill it into the fermentation tank at a temperature of 35℃, a water content of 42%, and a time of 72 hours. Stop the fermentation when the pH value drops to 4.2, transfer it to a 4℃ cold storage for granulation, and the particle size is 3mm to obtain a special fermented feed for leaf-eating herbaceous mulberry.

[0063] Example 4: A fermented feed specifically for leaf-eating herb Polygonum multiflorum and a preparation method thereof, comprising the following steps:

[0064] Prepare the following components: 35 parts by weight of edible grass, 8 parts by weight of the functional composition, 40 parts by weight of corn flour, 18 parts by weight of soybean meal, 10 parts by weight of bran, 5 parts by weight of molasses, 0.3 parts by weight of a composite fermentation agent, 1.2 parts by weight of calcium hydrogen phosphate, 0.8 parts by weight of stone powder, 0.5 parts by weight of salt, 0.6 parts by weight of a composite trace element, and 0.8 parts by weight of a composite vitamin.

[0065] The functional composition includes puffed amaranth seeds, bacterial cellulose, tannin, phytase, zein, and phospholipids; the tannin activity is 500 U / g, the phytase activity is 800 U / g; the mass ratio of zein to phospholipids is 10:4, and the ethanol concentration is 80% v / v; the ratio of the fortified amaranth seed carrier to the enzyme solution is 1 g:10 mL; the composite fermentation agent contains 5×10 Lactobacillus plantarum 10 CFU / g, Saccharomyces cerevisiae 2×10 9 CFU / g, Bacillus subtilis 3×10 9CFU / g; Cellulase is a composite enzyme system of endo-β-glucanase and exo-β-glucanase, with an enzyme activity ratio of 1:1.2;

[0066] S1.1. Prepare puffed amaranth seeds in a hot press puffing machine at a temperature of 150°C and a pressure of 0.8 MPa for 30 seconds.

[0067] S1.2. First, bacterial cellulose is mixed with deionized water to prepare a bacterial cellulose dispersion. After uniformly mixing the puffed amaranth seed particles with 5% w / v of the bacterial cellulose dispersion, the bacterial cellulose dispersion is added in an amount of 6% by weight of the puffed amaranth seeds. The mixture is freeze-dried to obtain fortified amaranth seeds.

[0068] S1.3, dissolving tannase and phytase in a 0.1 M, pH 5.2 acetate buffer at a mixing ratio of 1:1 to obtain an enzyme solution;

[0069] S1.4. Immerse the fortified amaranth seed carrier in the enzyme solution, evacuate to -0.08 MPa, maintain for 5 min, and pulse pressurize to 0.2 MPa at a frequency of 0.5 Hz for 3 cycles to obtain the enzyme carrier.

[0070] S1.5. Add the enzyme carrier to a 15% w / v zein-phospholipid ethanol solution and coat in a fluidized bed with an inlet air temperature of 35°C, an atomizing pressure of 0.8 bar, a spray rate of 2 mL / min, and a fluidizing air volume of 20 m 3 / h, the thickness of the coating layer is 30 μm, and a functional composition is obtained;

[0071] S2.1. Crush the leaf-eating grass, spray it with 0.5% w / w cellulase solution, and let it stand at 45°C for 6 hours to obtain pretreated leaf-eating grass;

[0072] S2.2. Mix corn flour, soybean meal, bran, calcium hydrogen phosphate, stone powder, salt, complex trace elements, and complex vitamins in a twin-shaft paddle mixer, add edible grass, molasses, and the functional composition, and stir evenly;

[0073] S2.3. Add 35℃ warm water into the composite fermentation agent at a ratio of 1:10, let it stand for 15 minutes for activation, spray the activated bacterial solution evenly into the mixture, continue stirring for 3 minutes, and then fill it into the fermentation tank at a temperature of 35℃, a water content of 42%, and a time of 72 hours. Stop the fermentation when the pH value drops to 4.2, transfer it to a 4℃ cold storage for granulation, and the particle size is 3mm to obtain a special fermented feed for leaf-eating herbaceous mulberry.

[0074] Example 5: A fermented feed specifically for leaf-eating herb Polygonum multiflorum and a preparation method thereof, comprising the following steps:

[0075] Prepare the following components: 35 parts by weight of edible grass, 8 parts by weight of the functional composition, 40 parts by weight of corn flour, 18 parts by weight of soybean meal, 10 parts by weight of bran, 5 parts by weight of molasses, 0.3 parts by weight of a composite fermentation agent, 1.2 parts by weight of calcium hydrogen phosphate, 0.8 parts by weight of stone powder, 0.5 parts by weight of salt, 0.6 parts by weight of a composite trace element, and 0.8 parts by weight of a composite vitamin.

[0076] The functional composition includes puffed amaranth seeds, bacterial cellulose, tannin, phytase, zein, and phospholipids; the tannin activity is 500 U / g, the phytase activity is 800 U / g; the mass ratio of zein to phospholipids is 10:4, and the ethanol concentration is 80% v / v; the ratio of the fortified amaranth seed carrier to the enzyme solution is 1 g:10 mL; the composite fermentation agent contains 5×10 Lactobacillus plantarum 10 CFU / g, Saccharomyces cerevisiae 2×10 9 CFU / g, Bacillus subtilis 3×10 9 CFU / g; Cellulase is a composite enzyme system of endo-β-glucanase and exo-β-glucanase, with an enzyme activity ratio of 1:1.2;

[0077] S1.1. Prepare puffed amaranth seeds in a hot press puffing machine at a temperature of 150°C and a pressure of 0.8 MPa for 30 seconds.

[0078] S1.2. First, bacterial cellulose is mixed with deionized water to prepare a bacterial cellulose dispersion. After uniformly mixing the puffed amaranth seed particles with 5% w / v of the bacterial cellulose dispersion, the bacterial cellulose dispersion is added in an amount of 8% by weight of the puffed amaranth seeds. The mixture is freeze-dried to obtain fortified amaranth seeds.

[0079] S1.3, dissolving tannase and phytase in a 0.1 M, pH 5.2 acetate buffer at a mixing ratio of 1:1 to obtain an enzyme solution;

[0080] S1.4. Immerse the fortified amaranth seed carrier in the enzyme solution, evacuate to -0.08 MPa, maintain for 5 min, and pulse pressurize to 0.2 MPa at a frequency of 0.5 Hz for 3 cycles to obtain the enzyme carrier.

[0081] S1.5. Add the enzyme carrier to a 15% w / v zein-phospholipid ethanol solution and coat the enzyme in a fluidized bed at an inlet air temperature of 35°C, an atomization pressure of 0.8 bar, a spray rate of 2 mL / min, a fluidizing air volume of 20 m3 / h, and a coating layer thickness of 30 μm to obtain a functional composition;

[0082] S2.1. Crush the leaf-eating grass, spray it with 0.5% w / w cellulase solution, and let it stand at 45°C for 6 hours to obtain pretreated leaf-eating grass;

[0083] S2.2. Mix corn flour, soybean meal, bran, calcium hydrogen phosphate, stone powder, salt, complex trace elements, and complex vitamins in a twin-shaft paddle mixer, add edible grass, molasses, and the functional composition, and stir evenly;

[0084] S2.3. Add 35℃ warm water into the composite fermentation agent at a ratio of 1:10, let it stand for 15 minutes for activation, spray the activated bacterial solution evenly into the mixture, continue stirring for 3 minutes, and then fill it into the fermentation tank at a temperature of 35℃, a water content of 42%, and a time of 72 hours. Stop the fermentation when the pH value drops to 4.2, transfer it to a 4℃ cold storage for granulation, and the particle size is 3mm to obtain a special fermented feed for leaf-eating herbaceous mulberry.

[0085] Comparative Example 1: The method of Example 5 was adopted without adding the functional composition.

[0086] Comparative Example 2: The method of Example 5 was adopted, but the tannase and phytase were not modified by puffing amaranth seeds.

[0087] Comparative Example 3: The method of Example 5 was adopted, but the tannase and phytase were not modified by the bacterial cellulose dispersion.

[0088] Comparative Example 4: The method of Example 5 was adopted, but tannase and phytase were not modified by alcohol-soluble protein-phospholipid.

[0089] The present invention adopts a functional composition to prepare a special fermented feed for leaf-eating grass and Polygonum multiflorum. The performance index test items and test standards of the special fermented feed for leaf-eating grass and Polygonum multiflorum are as follows:

[0090] Sixty healthy, 30-day-old piglets with similar body weight (25±2kg) were selected and randomly divided into two groups. The experimental group was fed with the fermented feed of the present invention, and the control group was fed with commercially available ordinary feed with the same nutritional level. The breeding environment was controlled, the pig house temperature was 22±2°C, the relative humidity was 60-70%, free feeding was combined with scheduled feeding, and the scheduled feeding time was 08:00, 14:00, and 20:00. The feed amount and residual feed amount were recorded every day. The piglets were weighed on an empty stomach on the first and 30th day of the test, and the daily weight gain (ADG) (g / day) was calculated as (final weight-initial weight) / number of test days; the actual feed intake was calculated, and the feed intake (ADFI) (g / day) was calculated as (total feed amount-total residual feed amount) / number of test days, and the feed-to-meat ratio was ADFI / ADG.

[0091] The fermented feeds prepared in Examples 1-5 and Comparative Examples 1-4 were tested using the above standards, and the data obtained are shown in Table 1:

[0092] Table 1 Performance data of Examples 1-5 and Comparative Examples 1-4

[0093]

[0094] The above data fully demonstrate that compared with comparative examples 1-4, embodiments 1-5 can fully show the effect of the functional composition on the fermented feed specially used for the leaf-eating herb Aconitum carmichaelii in improving the growth performance of Aconitum carmichaelii.

[0095] Since the present invention adopts a functional composition to prepare the fermented feed for leaf-eating grass Polygonum multiflorum, the performance of the fermented feed for leaf-eating grass Polygonum multiflorum is effectively improved by the functional composition, specifically as follows:

[0096] It can be seen from Examples 1-3 that with the continuous increase in the proportion of the components of the functional composition, the fermented feed specially used for leaf-eating amaranth significantly improves the growth performance of amaranth. With the continuous increase in the content of the functional composition, it means that the tannin loading capacity and the phytase loading capacity are increased, which directly enhances the ability to degrade anti-nutritional factors. The effective degradation of phytic acid releases more soluble phosphorus, and phosphorus can promote the amount of calcium deposition in bones. At the same time, the binding of tannins to proteins is blocked, the crude protein digestibility is effectively improved, and the absorption rate of essential amino acids is further improved. In addition, the puffed amaranth seeds, bacterial cellulose dispersion and of the functional composition can all be used as food sources for microorganisms. Therefore, the proportion of the functional composition is positively correlated with the growth performance.

[0097] It can be seen from Examples 3-5 that with the continuous increase in the content of bacterial cellulose dispersion, the growth performance of the leaf-eating amaranth is significantly improved by the fermented feed specially used for amaranth. The bacterial cellulose dispersion is cross-linked in the pores of the puffed amaranth seeds to form a three-dimensional network scaffold, which improves the compressive strength and the stability of the carrier structure. It can avoid structural collapse during fluidized bed coating and reduce the leakage of enzymes in gastric acid, effectively ensuring that more active enzymes reach the intestine to degrade anti-nutritional factors and prolong the duration of enzyme activity. Therefore, the bacterial cellulose dispersion significantly improves the stability of the carrier structure.

[0098] According to the above test experiments, the fermented feed specially prepared for the leaf-eating herb Polygonum multiflorum according to Example 5 has the best performance, so Example 5 is regarded as the best example;

[0099] By comparing Example 5 with Comparative Examples 1-4, it can be seen that:

[0100] In comparative example 1, no functional composition was added. The growth performance of Amur cortex was improved worse by the fermented feed specially made for Amur cortex with leaf-eating grass. The leaf-eating grass contains high doses of tannins and phytic acid. The lack of the functional composition leads to the formation of tannin-protein complexes. Tannins bind to feed proteins through hydrogen bonds and hydrophobic effects to form insoluble precipitates, which greatly reduces the protein digestibility. Phytic acid is easily strongly combined with Ca ions, Zn ions and Fe ions to form insoluble salts, thereby affecting the absorption and utilization of nutrients. The fermented feed specially made for Amur cortex with leaf-eating grass is poor in improving the growth performance of Amur cortex with leaf-eating grass.

[0101] The worse the growth performance of the special fermented feed of the leafy grass for the two-headed black-billed capon is improved, the amaranth seed is not puffed, the carrier adsorption capacity is lost, the enzyme loading amount is reduced, and the enzyme is directly added in the stomach acid, the conformation of the enzyme is irreversibly denatured, the survival rate is greatly reduced, and the puffed carrier adsorbed enzyme is protected due to the limited space.

[0102] The worse the growth performance of the special fermented feed of the leafy grass for the two-headed black-billed capon is improved, the amaranth seed is not puffed, the carrier adsorption capacity is lost, the enzyme loading amount is reduced, and the enzyme is directly added in the stomach acid, the conformation of the enzyme is irreversibly denatured, the survival rate is greatly reduced, and the puffed carrier adsorbed enzyme is protected due to the limited space. + The active enzyme is leaked by the crack penetration, and therefore the nanofiber network of the bacterial cellulose dispersion liquid provides important mechanical support.

[0103] The worse the growth performance of the special fermented feed of the leafy grass for the two-headed black-billed capon is improved, the amaranth seed is not puffed, the carrier adsorption capacity is lost, the enzyme loading amount is reduced, and the enzyme is directly added in the stomach acid, the conformation of the enzyme is irreversibly denatured, the survival rate is greatly reduced, and the puffed carrier adsorbed enzyme is protected due to the limited space.

[0104] In summary, the porous structure is formed by puffing the amaranth seed carrier at high temperature and high pressure, the enzyme loading amount is improved, the carrier skeleton is enhanced by cross-linking the bacterial cellulose dispersion liquid, the puffed carrier skeleton is enhanced, the corn protein- phospholipid coating is used to shield the erosion of the stomach acid and bile salt, the trinity enzyme targeted delivery system is adopted, the composite fermentation agent is used for synergistic fermentation, the precise degradation of the anti-nutritional factors in the intestinal tract is realized, the efficient utilization of the high-tannin raw material is realized, and the growth potential of the two-headed black-billed capon is fully released.

[0105] The basic principles, main features and advantages of the present application are shown and described above. Those skilled in the art should understand that the present application is not limited by the above examples, and the above examples and descriptions in the specification are only preferred examples of the present application and are not intended to limit the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the claimed present application.

Claims

1. A fermented feed specifically for leaf-eating herb Polygonum multiflorum, characterized by: The fermented feed for leaf-eating mulberry includes leaf-eating grass, functional composition, corn flour, soybean meal, bran, molasses, compound fermentation bacteria, calcium hydrogen phosphate, stone powder, salt, compound trace elements and compound vitamins. The functional composition comprises puffed amaranth seeds, bacterial cellulose, tannase, phytase, zein and lecithin.

2. The fermented feed for the leaf-eating herb Polygonum multiflorum according to claim 1, characterized in that: The invention comprises 25-35 parts by weight of the edible grass, 3-8 parts by weight of the functional composition, 30-40 parts by weight of corn flour, 12-18 parts by weight of soybean meal, 5-10 parts by weight of bran, 2-5 parts by weight of molasses, 0.1-0.3 parts by weight of a composite fermentation agent, 0.5-1.2 parts by weight of calcium hydrogen phosphate, 0.3-0.8 parts by weight of stone powder, 0.2-0.5 parts by weight of salt, 0.3-0.6 parts by weight of composite trace elements, and 0.4-0.8 parts by weight of composite vitamins.

3. The fermented feed for the leaf-eating herb Polygonum multiflorum according to claim 1, characterized in that: The composite fermentation agent contains 1-5×10 Lactobacillus plantarum 10 CFU / g, Saccharomyces cerevisiae 0.5-2×10 9 CFU / g, Bacillus subtilis 1-3×10 9 CFU / g.

4. The fermented feed for the leaf-eating herb Polygonum multiflorum according to claim 1, characterized in that: The complex trace elements include Fe, Zn, Cu, Mn, I, Se, and Co, and the complex vitamins include A, D3, E, K3, and B group.

5. The fermented feed for the leaf-eating herb Polygonum multiflorum according to claim 1, characterized in that: The preparation method of the functional composition is as follows: S1.

1. Prepare puffed amaranth seeds in a hot press puffing machine at a temperature of 150-180°C, a pressure of 0.6-0.8 MPa, and puffing for 15-30 seconds; S1.

2. First, bacterial cellulose is mixed with deionized water to prepare a bacterial cellulose dispersion. The puffed amaranth seed particles and the bacterial cellulose dispersion are uniformly mixed at a solid-to-liquid ratio of 1:2 to 5 g / mL, wherein the dry weight of the bacterial cellulose is 4-8% of the dry weight of the puffed amaranth seeds. The mixture is freeze-dried to obtain fortified amaranth seeds. S1.3, dissolving tannase and phytase in a 0.1 M acetic acid buffer solution with a pH of 4.8-5.2 at a mixing mass ratio of 1:0.5-1 to obtain an enzyme solution; S1.

4. Immerse the fortified amaranth seed carrier in the enzyme solution, evacuate to -0.08 MPa, maintain for 5 min, and pulse pressurize to 0.2 MPa at a frequency of 0.5 Hz for 3 cycles to obtain the enzyme carrier. S1.

5. Add the enzyme carrier to a 5-15% w / v zein-phospholipid ethanol solution and coat in a fluidized bed at an inlet air temperature of 35-40°C, an atomizing pressure of 0.8 bar, a spray rate of 2 mL / min, and a fluidizing air volume of 20 m 3 / h, the thickness of the coating layer is 10-30 μm, and a functional composition is obtained.

6. The fermented feed for the leaf-eating herb Polygonum multiflorum according to claim 5, characterized in that: The tannase activity is 500-800 U / g, and the phytase activity is 800-1200 U / g.

7. The fermented feed for the leaf-eating herb Polygonum multiflorum according to claim 5, characterized in that: The zein-phospholipid ethanol solution has a mass ratio of zein to phospholipid of 10:4-6 and an ethanol concentration of 80-85% v / v.

8. The fermented feed for Polygonum multiflorum according to claim 5, characterized in that: The ratio of the reinforced amaranth seed carrier to the enzyme solution is 1 g: 10-15 mL.

9. A method for preparing a fermented feed specifically for the leaf-eating herb Acanthopanax chinensis, for preparing the fermented feed specifically for the leaf-eating herb Acanthopanax chinensis according to any one of claims 1 to 8, characterized in that: The steps include: S2.

1. Crush the leaf-eating grass, spray it with 0.5-0.7% w / w cellulase solution, and let it stand at 40-45° C. for 5-6 hours to obtain pretreated leaf-eating grass; S2.

2. Mix corn flour, soybean meal, bran, calcium hydrogen phosphate, stone powder, salt, complex trace elements, and complex vitamins in a twin-shaft paddle mixer, add edible grass, molasses, and the functional composition, and stir evenly; S2.

3. Add 35℃ warm water into the composite fermentation agent at a ratio of 1:10, let it stand for 15 minutes for activation, spray the activated bacterial solution evenly into the mixture, continue stirring for 3 minutes, and then fill it into the fermentation tank at a temperature of 35±2℃ and a water content of 40-42% for 48-72 hours. Stop the fermentation when the pH value drops to 4.2-4.5, and transfer it to a 4℃ cold storage for granulation. The particle size is 3-4mm to obtain a special fermented feed for leaf-eating herbaceous blackbird.

10. The method for preparing the fermented feed specifically for the leaf-eating herb Polygonum multiflorum according to claim 9, characterized in that: The cellulase is a composite enzyme system of endo-beta-glucanase and exo-beta-glucanase, with an enzyme activity ratio of 1:0.8-1.2.

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