Feed additive for improving anti-stress ability, growth performance and immunocompetence of chicks as well as preparation method and application of feed additive

Abstract

The invention discloses a feed additive for improving anti-stress ability, growth performance and immunocompetence of chicks as well as a preparation method and application of the feed additive. The feed additive comprises an active component and a carrier, the active component contains taurine, vitamin C, vitamin E and beta-carotene; wherein the mass ratio of the taurine to the vitamin C to the vitamin E to the beta-carotene is (5-15): (1-3): (1-3): 1. According to the feed additive, through the synergistic effect of the added components, the anti-stress ability, the growth performance and the immunocompetence of chicks can be improved.

Description

Technical Field

[0001] The invention belongs to the field of feed additives, and particularly relates to a feed additive for improving the stress resistance, growth performance and immunity of chicks, as well as a preparation method and application thereof. Background Art

[0002] In broiler production, early nutritional status is crucial. Whether commercial chickens can maximize their genetic performance is closely related to their early nutrition and feeding management, particularly the balance and stability of nutrient intake, stress tolerance, and immune resistance during the brooding period. Establishing a healthy and stable physiological state from the chick stage ensures optimal growth performance in commercial broiler production.

[0003] Newly hatched chicks are sensitive to the temperature of the house because their down has poor insulation and their thermoregulation mechanisms are not fully developed. They lack the ability to adapt well, and need to maintain a suitable and constant temperature. Unsuitable temperatures can easily lead to stress. Their digestive systems are not yet fully developed, their gizzards have poor grinding capacity, and their enzyme systems are not fully developed, resulting in insufficient secretion of digestive enzymes and weak digestive capacity. Their immune systems are not fully developed, making them vulnerable to disease. Due to these physiological characteristics of chicks, it is crucial to prioritize their nutritional intake and feeding management to ensure they meet growth and development standards at all stages.

[0004] Patent publication number CN 117502576 A discloses a summer anti-stress feed additive for laying hens and its preparation method. The feed additive comprises the following components by weight: 2 parts lysine sulfate, 3 parts DL-methionine, 40 parts corn gluten meal, 0.1 part vitamin E, 0.2 part vitamin C, 1 part γ-aminobutyric acid, 1 part taurine, 1 part iron glycinate, 0.5 part zinc methionine, 0.1 part yeast selenium, 20 parts calcium formate, 20 parts montmorillonite, 3 parts bile acid, 2 parts Enterococcus faecalis, 1 part yeast cell wall polysaccharide, 1 part ethoxyquinoline, 1 part laying hen multivitamin, and 1 part laying hen multienzyme preparation. This feed additive has a complex composition and is primarily used for feeding laying hens, not chicks. Its purpose is to improve the stress resistance of laying hens and increase egg production and egg quality in high-temperature environments, but it does not improve disease resistance. Since chicks have weak immune systems, improving their immunity is more important.

[0005] Therefore, there is an urgent need to develop a feed additive that can improve the growth performance, stress resistance and disease resistance of chicks, so as to improve the growth performance and adaptability of chicks to the environment. Summary of the Invention

[0006] The technical problem to be solved by the present invention is to overcome the existing technical defects and provide a feed additive using taurine, vitamin C, vitamin E and beta-carotene as a compound, which can significantly improve the immune ability of chicks while taking into account the improvement of anti-stress ability and growth performance.

[0007] In order to solve the above technical problems, the technical solutions of the present invention are as follows: A feed additive comprising an active ingredient and a carrier; The active ingredients contain taurine, vitamin C, vitamin E and beta-carotene; Among them, the mass ratio of taurine, vitamin C, vitamin E and β-carotene is 30~50:5~10:5~10:5~10.

[0008] The additive of the present invention enhances the growth performance of chicks through the synergistic effects of components such as a growth enhancer and an anti-stress enhancer. It regulates the metabolism of substances such as energy, protein, and carbohydrates, effectively enhancing the growth function of chicks and reducing nutrient loss. Simultaneously, the additive of the present invention has multiple functions, including providing nutrients, regulating anti-stress ability, and improving immunity. While supplementing nutrients and improving growth performance, it also enhances anti-stress and immunity, thereby increasing the average daily weight gain and average body weight of chicks, reducing mortality, and increasing serum immunoglobulin and antibody titers.

[0009] Preferably, the mass ratio of taurine, vitamin C, vitamin E and β-carotene is 40-50:5-8:5-8:5-8.

[0010] Preferably, the carrier is one or more of corn gluten meal, wheat bran, and defatted rice bran, preferably corn gluten meal; The mass ratio of the active component to the carrier is 60-40:40-60.

[0011] The outstanding feature of the present invention is that taurine, vitamin C, vitamin E and beta-carotene are added in combination to chick feed, thereby improving the growth performance of the chicks and alleviating the stress state of the chicks. Broiler chicken breeding experiments have shown that the feed additive with a specific proportion of the present invention can improve the growth performance of broiler chickens for 14 days, and the body weight is increased by 23.56 g and 21.75 g respectively compared with the control group, the mortality rate is reduced by 1.67% and 3.33% respectively, the spleen index is increased by 0.11 g / kg and 0.09 g / kg respectively, and the serum IgG and IgM levels are also improved to a certain extent; the growth performance of broiler chickens for 21 days is improved, and the body weight is increased by 25.63 g and 23.65 g respectively compared with control groups 1 and 2, the mortality rate is reduced by 1.39%, and the serum antibody titer of the chicks is also improved to a certain extent; the weight gain from 8 to 35 days is increased by 62.64 g and 57.36 g respectively compared with control group 1 and control group 2, and the mortality rate is reduced by 1.67%. This indicates that adding the additive of the present invention to the chick diet can improve the growth performance of the chicks, relieve the stress state of the chicks and enhance the immune ability of the chicks.

[0012] The present invention also provides a method for preparing the feed additive, comprising the following steps: (1) Carrier preparation: sieve the carrier through a 10-20 mesh sieve and set aside; (2) Dilution: Vitamin E, vitamin C, β-carotene and taurine are sequentially added to the sieved carrier for dilution; (3) Mixing: Add all the diluted product from step (2) into a mixer and mix for 3-4 minutes. The coefficient of variation (CV) of the mixer should be less than 5%, thereby obtaining the feed additive.

[0013] The present invention also provides an application of the feed additive in chick feeding or feed preparation.

[0014] Preferably, the feed additive is used to improve the anti-stress ability, growth performance and immunity of chicks and reduce the mortality rate of chicks.

[0015] Preferably, the feed additive is used for feeding chicks aged 1 to 35 days.

[0016] Preferably, the feed additive is added to the basal diet in an amount of 1-3% of the mass of the basal diet.

[0017] Preferably, the basal diet is a commercial broiler early stage compound feed (for example, New Hope Liuhe's broiler early stage compound feed 610 or broiler early stage mixed feed 510, etc.), and the raw materials include corn or soybean meal.

[0018] Furthermore, the present invention also provides a compound feed, comprising a basic feed and the feed additive; The basic feed includes corn or soybean meal; The feed additive is 1-3% of the mass of the basic feed.

[0019] Compared with the prior art, the present invention has the following beneficial effects: The chick feed additive of the present invention improves the metabolic absorption capacity of chicks through the synergistic effect of the various components added, and provides corresponding nutritional supplements. It also increases the average daily weight gain and average body weight of chicks, reduces the feed-to-meat ratio, improves the chicks' stress resistance and growth performance, and more importantly, enhances the chicks' immunity and reduces mortality.

[0020] The specific functions of each component in the additive are as follows: Vitamin C, vitamin E, and taurine can reduce stress responses and the potential for stress in chicks, improving their growth performance. Taurine can relieve stress-related nervous tension and counteract excessive nervous excitation, while also increasing appetite and feed intake. β-carotene, vitamin E, vitamin C, and taurine all scavenge reactive oxygen free radicals.

[0021] At the same time, the present invention carries out a nutritional supplement design for the growth and development of chicks, which is rich in vitamins, amino acids, and trace elements. The carrier raw material adopts high-quality corn protein powder to increase the nutrient concentration of the feed, increase the protein and energy levels of the chick feed, and ensure the nutritional intake of the chicks. DETAILED DESCRIPTION

[0022] In order to facilitate understanding of the present invention, the present invention will be described in more detail below. The specific technical solution is as follows.

[0023] Comparative Example 1 Every 100 parts are composed of the following raw materials by weight: 50 parts of taurine, 5 parts of vitamin C, 5 parts of vitamin E, and the rest are carriers, and the carrier is corn gluten powder.

[0024] Comparative Example 2 Every 100 parts are composed of the following raw materials by weight: 5 parts of vitamin C, 5 parts of vitamin E, 10 parts of beta-carotene, and the rest are carriers, and the carrier is corn gluten powder.

[0025] Example 1 Every 100 parts are composed of the following raw materials by weight: 45 parts of taurine, 5 parts of vitamin C, 5 parts of vitamin E, 5 parts of beta-carotene, and the rest is a carrier, which is corn gluten powder.

[0026] The preparation steps of the additive include: S1, carrier preparation: sieve corn gluten powder through a 14-mesh sieve to serve as a carrier.

[0027] S2, dilution: using corn protein powder as a carrier, adding vitamin E, vitamin C, β-carotene and taurine in sequence for dilution treatment.

[0028] S3, mixing: add all the materials of the product diluted in S2 into a mixer (SLHSJ0.2 double-shaft mixer, coefficient of variation CV<5%) and mix for 4 minutes.

[0029] S4. The mixed finished product is packaged and sealed according to specifications for storage.

[0030] Example 2 Every 100 parts are composed of the following raw materials by weight: 30 parts of taurine, 10 parts of vitamin C, 10 parts of vitamin E, 10 parts of beta-carotene, and the rest is a carrier, which is corn gluten powder.

[0031] The preparation method is the same as that in Example 1, and the mixed tablets are packaged and sealed according to specifications for storage.

[0032] Effect Example 1 1.1 Test methods and grouping At the beginning of the experiment, 240 one-day-old commercial white-feathered broiler chickens (AA) with normal body shape and good health were selected. ＋ ) were randomly divided into four groups, each with six replicates, and ten broilers per replicate: Control Group 1 (basal diet + 2% control example 1), Control Group 2 (basal diet + 2% control example 2), Experimental Group 1 (basal diet + 2% example 1), and Experimental Group 2 (basal diet + 2% example 2). Data were measured after two weeks of feeding. The experimental basal diet was a commercial broiler early-stage feed. All diets used the same batch of commercial feed, with corn and soybean meal as the primary ingredients. The experiment was conducted at the animal breeding base of Zhejiang New Hope Co., Ltd. During the experiment, chickens had free access to food and water. Disinfection and immunization were performed as per routine procedures.

[0033] 1.2 Measurement indicators At 14 days of age, the chickens in each group were weighed in duplicate to calculate the average weight. Mortality and culling data were recorded daily.

[0034] Average weight = total weight of replicate chickens / number of replicate chickens Mortality rate = number of deaths / number of chicks entering the house × 100% Spleen index: After the poultry is killed by bleeding from the jugular vein, the spleen is dissected and the impurities and fascia are removed. After the moisture is absorbed by filter paper, the fresh weight is immediately weighed to calculate the immune organ index.

[0035] Serum IgG and IgM levels: Determine the content according to the instructions of the IgG and IgM test kits (Nanjing Jiancheng) 1.3 Statistical methods SPSS 23.0 statistical software was used to analyze the experimental data, and one-way ANOVA and Tukey's multiple test were used to compare the significance of the differences among the groups (P < 0.05).

[0036] 1.4 Test results The growth performance, immune indexes and other data of each group are shown in Table 1 below.

[0037] Table 1. Comparison of the effects of adding different doses of the additives of the present invention on 14-day-old broilers

[0038] Note: Different letters in the same industry indicate significant differences. P < 0.05).

[0039] 1.5 Results Analysis As can be seen from the table above, compared with control group 1 and control group 2, the average body weight 14 days after adding this additive was significantly improved. The average body weight of test group 1 increased by 23.56 and 21.75 g compared with control group 1 and control group 2, respectively, and the mortality rate decreased by 1.67% and 3.33% compared with control group 1 and control group 2, respectively. The spleen index of broiler chickens in test group 1 was significantly higher than that in the control group, increasing by 0.11 and 0.09 g / kg compared with control group 1 and control group 2, respectively. The levels of serum immunoglobulins IgG and IgM were both increased to varying degrees compared with the control group. After adding this additive, the broiler chickens in the test group showed that adding the additive of the present invention to chick feed can significantly improve the growth performance of chicks and enhance the immune and stress resistance of chicks.

[0040] Effect Example 2 2.1 Test methods and grouping 288 one-day-old commercial white-feathered broiler chickens (AA ＋ The broilers were randomly divided into four groups, each with six replicates, and each replicate consisted of 12 chickens: control group 1 (basal diet + 2% control example 1), control group 2 (basal diet + 2% control example 2), experimental group 1 (basal diet + 2% example 1), and experimental group 2 (basal diet + 2% example 2). Data were collected and measured after three weeks of feeding. All diets used the same batch of broiler early-stage feed, produced by Hongli Feed Co., Ltd., with corn and soybean meal as the main ingredients. The experiment was conducted at the animal breeding base of Zhejiang Xinhecheng Co., Ltd. During the experiment, feed and water were freely available, and disinfection and immunization were carried out according to routine procedures.

[0041] 2.2 Measurement indicators At 21 days of age, the chickens in each group were weighed in duplicate to calculate the average weight. Mortality and culling data were recorded daily.

[0042] Average daily weight gain = (final weight - initial weight) / number of test days Mortality rate = number of deaths / number of chicks entering the house × 100% Newcastle disease antibody titer: The Newcastle disease antibody titer of chickens was determined according to the "Newcastle disease diagnostic technology" (GB / T 16550-2008) standard. 2.3 Statistical methods SPSS 23.0 statistical software was used to analyze the experimental data, and one-way ANOVA and Tukey's multiple test were used to compare the significance of the differences among the groups (P < 0.05).

[0043] 2.4 Test results The growth performance, immune indexes and other data of each group are shown in Table 2 below.

[0044] Table 2. Comparison of the effects of adding different doses of the additives of the present invention on 21-day-old broilers

[0045] 2.5 Results Analysis As can be seen from the table above, the addition of this additive significantly increased the average weight of 21-day broiler chickens. The average weight of broiler chickens in test group 1 increased by 25.63g and 23.65g compared to control group 1 and control group 2, respectively. The mortality rate of test groups 1 and 2 decreased by 1.39%, and it also had a certain effect on increasing the Newcastle disease antibody titer in the serum of chicks. The test results show that adding the additive of the present invention to chick feed can significantly improve the growth performance of chicks, enhance the immunity and stress resistance of chicks, and reduce the mortality rate.

[0046] Effect Example 3 3.1 Test methods and grouping The breeding experiment was carried out in the broiler cage house of the animal breeding base of Zhejiang New Hope Co., Ltd., and 240 8-day-old commercial white-feathered broiler chickens (AA ＋ The experimental group was randomly divided into four groups, each with six replicates, and ten broilers per replicate: control group 1 (basal diet + 2% control example 1), control group 2 (basal diet + 2% control example 2), experimental group 1 (basal diet + 2% example 1), and experimental group 2 (basal diet + 2% example 2). The initial weight of the experimental chickens was approximately 165 g, and data were measured after four weeks of feeding. The experimental basal diet was produced by Zhejiang Xinhecheng Co., Ltd., and all diets used the same batch of raw materials and the same production process. During the experiment, the chickens had free access to food and water, and disinfection and immunization were performed as usual.

[0047] 3.2 Measurement indicators The weight of broiler chickens was recorded regularly every week, the condition of broiler chickens was observed regularly every day, and the number of deaths was recorded.

[0048] Average daily weight gain = (final weight - initial weight) / number of test days Mortality rate = number of deaths / number of chicks entering the house × 100% Feed-to-weight ratio = total feed consumed / total weight gain 3.3 Statistical methods SPSS 23.0 statistical software was used to analyze the experimental data, and one-way ANOVA and Tukey's multiple test were used to compare the significance of the differences among the groups (P < 0.05).

[0049] 3.4 Test results Table 3. Comparison of the effects of adding different doses of the additives of the present invention on broilers aged 8-35 days

[0050] 3.5 Results Analysis As shown in the table above, compared to the control group, after adding the additive, the average daily weight gain of broiler chickens in experimental group 1 from 8 to 35 days increased by 62.64 g and 57.36 g, respectively, compared to control groups 1 and 2, and the feed-to-weight ratio was significantly reduced. The mortality rate in experimental group 1 was also reduced by 3.33% compared to control group 2. This indicates that adding the additive of the present invention to the chick diet can improve the growth performance of chicks, alleviate stress in chicks, and reduce mortality.

Claims

1. A feed additive, characterized in that including active ingredients and carriers; The active ingredients contain taurine, vitamin C, vitamin E and beta-carotene; Among them, the mass ratio of taurine, vitamin C, vitamin E and β-carotene is 30~50:5~10:5~10:5~10.

2. The feed additive according to claim 1, characterized in that The mass ratio of taurine, vitamin C, vitamin E and beta-carotene is 40-50:5-8:5-8:5-8.

3. The feed additive according to claim 1 or 2, characterized in that The carrier is one or more of corn gluten meal, wheat bran, and defatted rice bran; The mass ratio of the active component to the carrier is 60-40:40-60.

4. The feed additive according to claim 3, characterized in that The carrier is corn gluten meal.

5. A method for preparing the feed additive according to any one of claims 1 to 4, characterized in that: The following steps are involved: (1) Carrier preparation: sieve the carrier through a 10-20 mesh sieve and set aside; (2) Dilution: Vitamin E, vitamin C, β-carotene and taurine are sequentially added to the sieved carrier for dilution; (3) Mixing: Add all the materials diluted in step (2) into a mixer and mix for 3-4 minutes. The coefficient of variation (CV) of the mixer is less than 5%, thereby obtaining the feed additive.

6. Use of the feed additive according to any one of claims 1 to 4 or the feed additive obtained by the preparation method according to claim 5 in chick feeding or feed preparation.

7. The use of the feed additive according to claim 6 in chick feeding or feed preparation, characterized in that, The feed additive is used for improving the anti-stress ability, growth performance and immunity of chicks and reducing the mortality rate of chicks.

8. Use of the feed additive according to claim 6 in chick feeding or feed preparation, characterized in that, The feed additive is used for feeding chicks aged 1 to 35 days.

9. Use of the feed additive according to claim 6 in chick feeding or feed preparation, characterized in that, The feed additive is added to the basic diet, and the added amount is 1-3% of the basic diet weight; The basic diet is a commercial broiler early-stage compound feed, and the raw materials include corn or soybean meal.

10. A compound feed, characterized in that: Comprising a basic feed and the feed additive according to any one of claims 1 to 4 or the feed additive obtained by the preparation method according to claim 5; The basal feed includes corn or soybean meal.

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