Antibacterial composition, preparation method and application

Abstract

The invention belongs to the technical field of skin care products, and relates to an antibacterial composition, a preparation method and application, the antibacterial composition comprises the following components by weight: 5-20 parts of American ginseng seed polysaccharide nano-selenium; the feed additive is prepared from, by weight, 5-20 parts of sweet wormwood herb extract, 5-20 parts of folium artemisiae argyi extract, 5-20 parts of perilla frutescens extract, 5-20 parts of mulberry leaf extract, 5-20 parts of roxburgh rose extract and 3-8 parts of crab shell powder. The American ginseng seed polysaccharide nano-selenium comprises American ginseng seed polysaccharide, sodium selenite and vitamin C. The American ginseng seed polysaccharide nano-selenium is added into the antibacterial composition, and the antibacterial composition is a novel nano-material formed by compounding nano-selenium and polysaccharide; nano-selenium has the characteristics of high biological activity and low natural toxicity, polysaccharide has anti-inflammatory, antibacterial and anti-oxidation effects and the like, and the addition of American ginseng seed polysaccharide nano-selenium can exert the dual effects of polysaccharide and selenium, so that the anti-inflammatory, antibacterial and anti-oxidation effects of the skin care product are stronger.

Description

Technical Field

[0001] The present invention belongs to the technical field of skin care products and relates to an antibacterial composition, a preparation method and an application thereof. Background Art

[0002] As people's living standards improve, they are paying more and more attention to skin care. However, the antibacterial ingredients in skin care products currently on the market are usually mainly chemical substances. As the active ingredients, long-term use of chemical drugs will inevitably cause different degrees of impact on users, such as skin irritation and the development of bacterial resistance.

[0003] Staphylococcus aureus and Staphylococcus epidermidis belong to the genus Staphylococcus, are representatives of Gram-positive bacteria, and are commonly found on the skin, causing a variety of skin diseases. For example, S. aureus can directly increase serum protease activity, leading to skin barrier damage; while S. epidermidis can, under certain conditions, cause suppurative infections and even sepsis. These two bacteria produce different pigments: S. aureus produces golden-yellow colonies, while S. epidermidis produces white colonies.

[0004] Therefore, there is a need for a skin care product that is safe and non-toxic to the human body, has no irritation to the skin, and has strong antibacterial ability and antibacterial effect. Summary of the Invention

[0005] In order to solve the above technical problems, the present invention provides an antibacterial composition, a preparation method and an application thereof.

[0006] The specific technical solutions are as follows:

[0007] The first object of the present invention is to provide an antibacterial composition comprising the following components in parts by weight:

[0008] 5-20 parts of American ginseng seed polysaccharide nano-selenium; 5-20 parts of Artemisia annua extract, 5-20 parts of Artemisia argyi extract, 5-20 parts of Perilla frutescens extract, 5-20 parts of mulberry leaf extract, 5-20 parts of Rosa roxburghii extract, and 3-8 parts of crab shell powder;

[0009] The Artemisia annua extract, the Artemisia argyi extract, the Perilla frutescens extract, the Mulberry leaf extract, and the Rosa roxburghii extract are all dried aqueous extracts;

[0010] The American ginseng seed polysaccharide nano-selenium comprises American ginseng seed polysaccharide, sodium selenite and vitamin C.

[0011] The American ginseng seed polysaccharide nano-selenium in the antibacterial composition of the present invention is a new type of nanomaterial composed of nano-selenium and polysaccharide; nano-selenium has the characteristics of high biological activity and low natural toxicity, and polysaccharide has anti-inflammatory, antibacterial, antioxidant and other effects. The addition of American ginseng seed polysaccharide nano-selenium can play the dual role of polysaccharide and selenium, and enhance the anti-inflammatory, antibacterial and antioxidant effects of the composition; Artemisia annua has the effects of clearing heat, cooling blood, relieving summer heat, dispelling wind and relieving itching; mugwort has antibacterial and anti-inflammatory effects, and contains multiple antibacterial ingredients, which are effective against a variety of bacteria and fungi It has an inhibitory effect and is often used to treat skin infections, oral infections, etc.; mulberry leaves are rich in flavonoids and have strong antioxidant capacity; sea buckthorn has anti-inflammatory and antibacterial effects, can effectively relieve erythema and swelling caused by eczema, and can also accelerate the healing of wounds such as trauma and ulcers; crab shell powder has the effects of dispersing blood stasis, stopping bleeding, detoxifying and reducing swelling; rosmarinic acid, anthocyanins and other ingredients in perilla leaves can scavenge free radicals. Studies have shown that its extracts have a certain inhibitory effect on skin inflammation and allergic reactions. External application of crushed compresses or bathing can help relieve itching.

[0012] Furthermore, the mass ratio of the Artemisia annua extract obtained by water extraction and drying to Artemisia annua is 1:(25-40); the mass ratio of the Artemisia annua extract obtained by water extraction and drying to Artemisia annua is 1:(25-40); the mass ratio of the Perilla frutescens extract obtained by water extraction and drying to Perilla frutescens is 1:(25-40); the mass ratio of the mulberry leaf extract obtained by water extraction and drying to mulberry leaf is 1:(25-40); the mass ratio of the Rosa roxburghii extract obtained by water extraction and drying to Rosa roxburghii is 1:(25-40).

[0013] Furthermore, the preparation method of the American ginseng seed polysaccharide nano-selenium comprises the following steps:

[0014] The American ginseng seeds were dried and ground into powder, and the extraction solvent was water with a solid-liquid ratio of 1:(5-20). The mixture was heated at 50-70°C for 2-4 hours, the residue was filtered, and the supernatant was added with ethanol for precipitation. The final ethanol concentration was 60% by volume. The precipitate was collected and dissolved in water and freeze-dried to obtain American ginseng seed polysaccharide.

[0015] Furthermore, the mass ratio of the American ginseng seed polysaccharide, sodium selenite and vitamin C is (10-20):1:(3-5).

[0016] Furthermore, the preparation method of the American ginseng seed polysaccharide nano-selenium comprises the following steps:

[0017] The American ginseng seed polysaccharide is configured into an American ginseng seed polysaccharide aqueous solution with a concentration of 2-5 mg / mL, sodium selenite is configured into an aqueous sodium selenite solution with a concentration of 1-3 mg / mL, and vitamin C is configured into an aqueous vitamin C (Vc) solution with a concentration of 5-10 mg / mL; then the American ginseng seed polysaccharide aqueous solution, the sodium selenite aqueous solution and the vitamin C aqueous solution are mixed, and the reaction is carried out at room temperature for 5-10 hours. After the overall reaction is completed, the liquid is directly freeze-dried to obtain American ginseng seed polysaccharide nano-selenium.

[0018] The second object of the present invention is to provide a method for preparing the above-mentioned antibacterial composition, comprising the following steps:

[0019] Dissolve American ginseng seed polysaccharide nano-selenium, artemisia annua extract, mugwort leaf extract, perilla extract, mulberry leaf extract, roxburghii extract and crab shell powder in 5-20 parts of water, stir thoroughly to dissolve, centrifuge and freeze-dry the supernatant to prepare an antibacterial composition.

[0020] The third object of the present invention is to provide the use of the above antibacterial composition in the preparation of skin care products.

[0021] Furthermore, the concentration of the antibacterial composition in the preparation of skin care products is greater than 0.5 mg / ml; preferably, greater than 1 mg / ml.

[0022] Furthermore, the skin care product is a skin care ointment.

[0023] Furthermore, the skin care ointment also includes azone, ethyl hydroxybenzoate, anhydrous ethanol, carbomer, sodium hyaluronate, glycerin, and triethanolamine.

[0024] Furthermore, the preparation method of the ointment comprises the following steps:

[0025] (1) dissolving American ginseng seed polysaccharide nano-selenium, Artemisia annua extract, Artemisia argyi extract, Perilla frutescens extract, Mulberry leaf extract, Rosa roxburghii extract and crab shell powder in 5-20 parts of water, stirring and dissolving them thoroughly to obtain an antibacterial composition raw material liquid;

[0026] (2) Add 2-5 parts of carbomer to 40-60 parts of water and allow to swell for 2-6 hours; prepare 2-5 parts of a 2-5 wt% sodium hyaluronate solution;

[0027] (4) taking the swollen carbomer, sodium hyaluronate solution, and 0.2-1.0 parts of glycerol and stirring evenly to obtain a carbomer mixed solution;

[0028] (5) Dissolve 0.5-1.0 parts of azone and 0.1-0.3 parts of ethylparaben in 2-3 parts of anhydrous ethanol, mix well, add to the carbomer mixed solution, then add 0.05-0.10 parts of triethanolamine and stir into a gel, finally add the prepared antibacterial composition raw material solution and mix well to obtain an antibacterial composition ointment.

[0029] The beneficial effects of the present invention are:

[0030] The present invention obtains a composition that is safe and non-toxic to the human body, non-irritating to the skin, and has strong anti-inflammatory, antibacterial and antioxidant properties through the synergistic effect of American ginseng seed polysaccharide nano-selenium with Artemisia annua extract, Artemisia argyi extract, Perilla frutescens extract, mulberry leaf extract, Rosa roxburghii extract and crab shell powder. The composition can be used in the preparation of skin care products. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 Schematic diagram of the drug sensitivity test results of different concentrations of the antibacterial composition raw material solution in Example 1 of the present invention;

[0032] A: Staphylococcus aureus; B: Staphylococcus epidermidis

[0033] Figure 2 Growth curves of Staphylococcus epidermidis in antibacterial composition raw material solutions of different concentrations in Example 1 of the present invention;

[0034] Figure 3 DPPH radical scavenging rate diagram of different concentrations of the antibacterial composition raw material solution in Example 1 of the present invention;

[0035] Figure 4 Graph showing the hydroxyl radical scavenging ability of the antibacterial composition raw material solution at different concentrations in Example 1 of the present invention;

[0036] Figure 5 ABTS free radical scavenging ability of different concentrations of the antibacterial composition raw material solution of Example 1 of the present invention;

[0037] Figure 6 Superoxide anion radical scavenging ability of the antibacterial composition raw material solution of different concentrations in Example 1 of the present invention;

[0038] Figure 7 Growth curves of Staphylococcus epidermidis in the raw material solution of the antibacterial composition at a concentration of 1 mg / mL in Example 1 of the present invention and the comparative example;

[0039] Figure 8 DPPH radical scavenging rate graph of the antibacterial composition raw material solution at a concentration of 1 mg / mL in Example 1 of the present invention and the comparative example;

[0040] Figure 9Graph showing the hydroxyl radical scavenging ability of the antibacterial composition raw material solution at a concentration of 1 mg / mL in Example 1 of the present invention and the comparative example;

[0041] Figure 10 ABTS free radical scavenging ability of the antibacterial composition raw material solution at a concentration of 1 mg / mL of Example 1 of the present invention and the comparative example;

[0042] Figure 11 The superoxide anion radical scavenging ability of the antibacterial composition raw material solution at a concentration of 1 mg / mL in Example 1 of the present invention and the comparative example;

[0043] Figure 12 Product image of the antibacterial composition ointment of the present invention. DETAILED DESCRIPTION

[0044] Unless otherwise specified, the instruments, reagents, and materials used in the following examples are all conventional instruments, reagents, and materials available in the prior art and can be obtained through regular commercial channels. The experimental methods and detection methods used in the following examples are all conventional experimental methods and detection methods available in the prior art, unless otherwise specified.

[0045] Preparation of American ginseng seed polysaccharide:

[0046] (1) Dry and grind American ginseng seeds, use water as the extraction solvent, and heat at 60°C for 3 hours at a solid-liquid ratio of 1:10. Filter the residue, take the supernatant, add ethanol with a final concentration of 60% by volume, and precipitate. Collect the precipitate, dissolve it in water, and freeze-dry it to obtain American ginseng seed polysaccharide;

[0047] Preparation of American ginseng seed polysaccharide nanoselenium:

[0048] (2) The American ginseng seed polysaccharide is configured into an American ginseng seed polysaccharide aqueous solution with a concentration of 2 mg / mL, the sodium selenite is configured into a sodium selenite aqueous solution with a concentration of 2 mg / mL, and the vitamin C is configured into a vitamin C (Vc) aqueous solution with a concentration of 8 mg / mL; then the American ginseng seed polysaccharide aqueous solution, the sodium selenite aqueous solution and the vitamin C aqueous solution are mixed in a volume ratio of 15:1:1, and the mixture is reacted at room temperature for 6 hours. After the overall reaction is completed, the liquid is directly freeze-dried to obtain American ginseng seed polysaccharide nano-selenium.

[0049] The American ginseng seed polysaccharide and American ginseng seed polysaccharide nano-selenium were used in the preparation of the following examples and comparative examples.

[0050] Example 1

[0051] Preparation of antibacterial composition:

[0052] Dissolve 10 parts each of American ginseng seed polysaccharide nano-selenium, Artemisia annua extract, Artemisia argyi extract, Perilla frutescens extract, Mulberry leaf extract, and Rosa roxburghii extract, and 5 parts of crab shell powder, by weight, in 10 parts of water, stir thoroughly to dissolve, centrifuge, collect the supernatant, and freeze-dry to obtain an antibacterial composition;

[0053] Among them, the roxburgh thorn extract: Kangze Department Store Retail Store, Xincheng District, Xi'an, the material ratio is 30:1, that is, the mass ratio of roxburgh thorn to the obtained water-extracted product roxburgh thorn extract is 30:1;

[0054] Artemisia annua extract: obtained from Faqi Chemical Products Store, Xi'an High-tech Zone, with a material ratio of 30:1, i.e., the mass ratio of Artemisia annua to the obtained water-extracted Artemisia annua extract was 30:1;

[0055] Perilla extract: Xi'an High-tech Zone Faqi Chemical Products Store, material ratio: 30:1, that is, the mass ratio of perilla to the obtained water-extracted perilla extract is 30:1;

[0056] Artemisia annua extract: Xi'an High-tech Zone Faqi Chemical Products Store, material ratio: 30:1, that is, the mass ratio of artemisia annua to the obtained water-extracted artemisia annua extract is 30:1;

[0057] Mulberry leaf extract: Xi'an High-tech Zone Faqi Chemical Products Store, material ratio: 30:1, that is, the mass ratio of mulberry leaves to the obtained water extraction product mulberry leaf extract is 30:1.

[0058] Artemisia annua extract, mugwort leaf extract, perilla extract, mulberry leaf extract and roxburghii roxburghii extract are the dried substances of the corresponding water extracts.

[0059] Comparative Example 1:

[0060] Compared with Example 1, this comparative example does not contain American ginseng seed polysaccharide nano-selenium. Other aspects are the same as Example 1 and will not be repeated here.

[0061] Comparative Example 2:

[0062] Compared with Example 1, this comparative example does not contain mugwort extract. Other details are the same as those in Example 1 and will not be repeated here.

[0063] Comparative Example 3:

[0064] Compared with Example 1, this comparative example does not contain mulberry leaf extract. Other details are the same as those in Example 1 and will not be repeated here.

[0065] Comparative Example 4:

[0066] Compared with Example 1, this comparative example does not contain the roxburghii extract. Other details are the same as those in Example 1 and will not be repeated here.

[0067] Comparative Example 5:

[0068] Compared with Example 1, this comparative example does not contain the perilla leaf extract. Other details are the same as those in Example 1 and will not be repeated here.

[0069] Experimental test:

[0070] 1. The antibacterial composition obtained in Example 1 was used as a raw material powder, and then diluted with water to liquids with different concentrations of 0.125 mg / mL, 0.25 mg / mL, 0.5 mg / mL, 0.75 mg / mL and 1 mg / mL as antibacterial composition raw material solutions; specific efficacy: antibacterial and antioxidant tests were performed.

[0071] 1. Drug sensitivity test:

[0072] Methods: Staphylococcus aureus and Staphylococcus epidermidis were used as research objects. 100 μL of 1×10 6 The two bacterial solutions with different cfu / mL were evenly spread on LB solid culture medium. After standing at room temperature for 5-10 minutes, sterile tweezers were used to pick up drug-sensitive paper and evenly stick them on the culture medium. 10 μL of raw material solution of different concentrations (filtered through 0.22 μm water system microporous filter membrane) was dropped on the drug-sensitive paper, and the size of the inhibition zone was observed after incubation at 37 ° C overnight.

[0073] Conclusion: Taking Staphylococcus aureus and Staphylococcus epidermidis as research objects, Figure 1 As shown in the drug sensitivity test results, the raw material solution had an inhibitory effect on Staphylococcus aureus at 1 mg / mL, but had no significant inhibitory effect at 0.25 mg / mL and 0.5 mg / mL. However, it had a significant inhibitory effect on Staphylococcus epidermidis at 0.25 mg / mL, 0.5 mg / mL, and 1 mg / mL, and the inhibitory effect was concentration-dependent. Therefore, Staphylococcus epidermidis was selected as the subject for subsequent experiments.

[0074] 2. Growth Curve Determination of Staphylococcus epidermidis

[0075] Experimental method: Staphylococcus epidermidis was placed in 1 mL of LB broth and cultured at 37°C with a shaker at 200 rpm for about 16 hours. The overnight culture was diluted to 1:100 in LB broth and adjusted to about 1×10 6 CFUs / mL. Add different concentrations of stock solution to a 96-well plate and mix with an equal volume of bacterial dilution solution. Use a microplate reader to draw a growth curve at 37°C and OD600nm at certain intervals, such as Figure 2 shown.

[0076] Conclusion: Compared with the control group, the addition of the raw material solution significantly inhibited bacterial proliferation, and the inhibitory effect became more pronounced as the concentration of the raw material solution increased. The strongest inhibitory effect was achieved at a concentration of 1.0 mg / mL, indicating that the raw material solution had a significant antibacterial effect. The difference between the control group and the experimental group was that the control group did not contain the raw material solution at different concentrations, but only bacteria.

[0077] 3 Antioxidant activity detection

[0078] 3.11,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity

[0079] Prepare stock solutions at 0.125 mg / mL, 0.25 mg / mL, 0.5 mg / mL, 0.75 mg / mL, and 1.0 mg / mL. Mix 900 μL of a 0.25 mmol / L DPPH free radical methanol solution with 100 μL of the stock solutions at different mass concentrations. Continue the reaction at 25°C for 1 hour. Take 200 μL of the reaction product and measure the absorbance at 517 nm using a microplate reader. The formula for calculating the DPPH free radical scavenging rate of the stock solution is as follows:

[0080]

[0081] Wherein: A0 is the absorbance of the DPPH free radical solution without the raw material solution; A2 is the absorbance of the mixture of the raw material solution and the DPPH free radical solution; A1 is the absorbance of the sample without the DPPH free radical solution.

[0082] 3.2 Hydroxyl radical scavenging activity

[0083] Hydroxyl radicals were generated using the Fenton reaction. A 2 mmol / L FeSO4 solution was mixed with a 1 mmol / L H2O2 solution, and the mixture was incubated at 25°C in the dark for 3 hours. 900 μL of the hydroxyl radical solution was mixed with 100 μL of 0.125 mg / mL, 0.25 mg / mL, 0.5 mg / mL, 0.75 mg / mL, and 1.0 mg / mL raw material solutions for 1 hour. The reacted solution was mixed with a salicylic acid solution (5 mmol / L) at a ratio of 1:1. After a color reaction of 5 minutes, the absorbance of the reaction product was measured at 510 nm using a microplate reader. An equal amount of ultrapure water was used to replace the H2O2 in the blank sample. The hydroxyl radical scavenging rate of the sample was calculated as follows:

[0084]

[0085] Where: A0 is the absorbance of ultrapure water instead of the sample; A1 is the absorbance of the test sample.

[0086] 3.3 ABTS free radical scavenging activity

[0087] Accurately weigh 192.3 mg of ABTS reagent and 0.0662 g of potassium persulfate respectively. Dilute the ABTS reagent to a 50 mL volumetric flask with deionized water to a concentration of 7 mmol / L. Dilute potassium persulfate to 100 mL to a concentration of 2.45 mmol / L. Take the same volume of the two solutions, mix them evenly, and place them in the refrigerator to react for 14 hours to obtain the ABTS stock solution. Then mix the ABTS stock solution with anhydrous ethanol in a ratio of 2:92 so that its absorbance at 734 nm is 0.7±0.02. Absorb 0.4 mL of raw material solutions with concentrations of 0.125, 0.25 mg / mL, 0.5 mg / mL, 0.75 mg / mL, and 1.0 mg / mL and add 3.6 mL of ABTS working solution. After mixing, protect from light and react for 10 minutes. Use an enzyme reader to measure the absorbance B1 at 734 nm, and use Vc as a positive control. The formula for calculating the ABTS free radical scavenging rate is as follows:

[0088]

[0089] Where: B1 is the absorbance of the experimental group; B0 is the absorbance of the blank group

[0090] 3.4 Superoxide anion radical scavenging activity

[0091] 1 mL each of the stock solution and Vc solution at concentrations of 0.125, 0.25 mg / mL, 0.5 mg / mL, 0.75 mg / mL, and 1.0 mg / mL was placed in a test tube. 3 mL of Tris-HCl solution (0.1 mol / L) was added and heated in a 37°C water bath for 10 minutes. 3 mL of pyrogallic acid solution (5 mmol / L) was added and reacted for 4 minutes. 1 mL of hydrochloric acid was immediately added to terminate the reaction. The absorbance was measured at a wavelength of 320 nm. The superoxide anion radical scavenging rate was calculated as follows:

[0092]

[0093] Where: A2 is the absorbance of the sample solution; A1 is the background absorbance of the sample; A0 is the absorbance of the blank control.

[0094] in conclusion

[0095] DPPH free radical is a relatively stable free radical and is widely used to evaluate the ability of various antioxidants to scavenge free radicals. The scavenging ability of the raw material solution on DPPH free radicals in the concentration range of 0.125-1 mg / mL was determined. The results are as follows: Figure 3As shown in the figure, the scavenging ability of the raw material solution on DPPH free radicals increased in a dose-dependent manner. At the concentrations of 0.125, 0.25 mg / mL, 0.5 mg / mL, 0.75 mg / mL and 1.0 mg / mL, the scavenging rates on DPPH free radicals were 6.92%, 16.12%, 21.84%, 47% and 58.53%, respectively. Figure 4 As shown in the figure, the raw material solution also has a significant ability to scavenge hydroxyl free radicals. At concentrations of 0.125, 0.25 mg / mL, 0.5 mg / mL, 0.75 mg / mL, and 1.0 mg / mL, the scavenging rates are 5.16%, 10.97%, 13.83%, 37.48%, and 62.60%, respectively. Figure 5 As shown in the figure, the scavenging rates of ABTS free radicals were 3.09%, 14.61%, 38.89%, 50.26% and 72.17%, while the scavenging rate of Vc for ABTS free radicals was 77.62%. The raw material solution had a significant effect at a concentration of 1.0 mg / mL. In addition, Figure 6 As shown, the scavenging rates of the raw materials against superoxide anion radicals were 11.06%, 33.95%, 44.14%, 54.42%, and 65.38%, respectively. The concentration of VC was 0.25 mg / mL. As an antioxidant, it is a common positive control in experiments. Comparing the samples with VC provides a more intuitive view of the antioxidant capacity of the raw materials at different concentrations.

[0096] 2. The antibacterial compositions obtained in Example 1 and the comparative example were used as raw material powders, and diluted with water to different concentrations of 0.125 mg / mL, 0.25 mg / mL, 0.5 mg / mL, 0.75 mg / mL and 1 mg / mL as antibacterial composition raw material solutions; specific efficacy: antibacterial and antioxidant tests were carried out.

[0097] 1. The growth curves of Staphylococcus epidermidis in the antibacterial composition raw material solution at a concentration of 1 mg / mL in Example 1 and Comparative Example are as follows: Figure 7 shown.

[0098] 2. The results of the DPPH radical scavenging activity test of the antibacterial composition raw material solutions of different concentrations in Example 1 and the comparative example are shown in Table 1; the results of the DPPH radical scavenging activity test of the antibacterial composition raw material solutions of Example 1 and the comparative example at a concentration of 1 mg / mL are shown in Table 1. Figure 8 shown.

[0099] Table 1 DPPH free radical scavenging activity test results

[0100]

[0101] 3. The results of the detection of the hydroxyl radical scavenging activity of the antibacterial composition stock solution of Example 1 and Comparative Example at different concentrations are shown in Table 2; the results of the detection of the hydroxyl radical scavenging activity of the antibacterial composition stock solution of Example 1 and Comparative Example 1 at a concentration of 1 mg / mL are shown in Table 2. Figure 9

[0102] Table 2: Results of the detection of the hydroxyl radical scavenging activity

[0103]

[0104] 4. The results of the detection of the ABTS radical scavenging activity of the antibacterial composition stock solution of Example 1 and Comparative Example at different concentrations are shown in Table 3; the results of the detection of the ABTS radical scavenging activity of the antibacterial composition stock solution of Example 1 and Comparative Example 1 at a concentration of 1 mg / mL are shown in Table 3. Figure 10

[0105] Table 3: Results of the detection of the ABTS radical scavenging activity

[0106]

[0107] 5. The results of the detection of the superoxide anion radical scavenging activity of the antibacterial composition stock solution of Example 1 and Comparative Example at different concentrations are shown in Table 4; the results of the detection of the superoxide anion radical scavenging activity of the antibacterial composition stock solution of Example 1 and Comparative Example 1 at a concentration of 1 mg / mL are shown in Table 4. Figure 11

[0108] Table 4: Results of the detection of the superoxide anion radical scavenging activity

[0109]

[0110]

[0111] Application Example:

[0112] Preparation of a skin care ointment:

[0113] (1) Dissolve 10 mg of each of American ginseng seed polysaccharide nano selenium, artemisia extract, wormwood extract, perilla extract, mulberry leaf extract, and hibiscus extract, and 5 mg of crab shell powder in 10 mL of water, and thoroughly stir and dissolve, to obtain an antibacterial composition stock solution;

[0114] (2) Take 2.5 g of carbomer and add to 50 mL of water, and swell for 3 hours;

[0115] (3) Prepare a 2.5% sodium hyaluronate solution of 2.5 g;

[0116] (4) Thoroughly stir and mix the swelled carbomer, the sodium hyaluronate solution, and 0.5 g of glycerol, to obtain a carbomer mixture solution;​​​

[0117] (5) Take 1g of azone and 0.15g of ethylparaben and dissolve them in 2.5ml of anhydrous ethanol, mix them evenly, add them to the carbomer mixed solution, then add 0.075g of triethanolamine and stir them into a gel, finally add the prepared antibacterial composition raw material solution and mix them evenly to obtain the antibacterial composition ointment, put it into a tube, and the product is as follows: Figure 12 The product is simple and convenient to use. After cleaning the skin, dry the surface and apply an appropriate amount to the corresponding area.

[0118] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. An antibacterial composition, characterized in that The composition comprises the following components in parts by weight: 5-20 parts of American ginseng seed polysaccharide nano-selenium; 5-20 parts of Artemisia annua extract, 5-20 parts of Artemisia argyi extract, 5-20 parts of Perilla frutescens extract, 5-20 parts of mulberry leaf extract, 5-20 parts of Rosa roxburghii extract, and 3-8 parts of crab shell powder; The Artemisia annua extract, the Artemisia argyi extract, the Perilla frutescens extract, the Mulberry leaf extract, and the Rosa roxburghii extract are all dried aqueous extracts; The American ginseng seed polysaccharide nano-selenium comprises American ginseng seed polysaccharide, sodium selenite and vitamin C.

2. The antibacterial composition according to claim 1, characterized in that The preparation method of American ginseng seed polysaccharide nano-selenium comprises the following steps: The American ginseng seeds were dried and ground into powder, and the extraction solvent was water with a solid-liquid ratio of 1:(5-20). The mixture was heated at 50-70°C for 2-4 hours, the residue was filtered, and the supernatant was added with ethanol for precipitation. The final ethanol concentration was 60% by volume. The precipitate was collected and dissolved in water and freeze-dried to obtain American ginseng seed polysaccharide.

3. The antibacterial composition according to claim 1, characterized in that The mass ratio of the American ginseng seed polysaccharide, sodium selenite and vitamin C is (10-20):1:(3-5).

4. The antibacterial composition according to claim 3, characterized in that The preparation method of American ginseng seed polysaccharide nano-selenium comprises the following steps: The American ginseng seed polysaccharide is configured into an American ginseng seed polysaccharide aqueous solution with a concentration of 2-5 mg / mL, sodium selenite is configured into an aqueous sodium selenite solution with a concentration of 1-3 mg / mL, and vitamin C is configured into an aqueous vitamin C solution with a concentration of 5-10 mg / mL. The American ginseng seed polysaccharide aqueous solution, the sodium selenite aqueous solution and the vitamin C aqueous solution are then mixed and reacted at room temperature for 5-10 hours. After the overall reaction is completed, the liquid is directly freeze-dried to obtain American ginseng seed polysaccharide nano-selenium.

5. A method for preparing the antibacterial composition according to any one of claims 1 to 4, characterized in that: The steps include: Dissolve American ginseng seed polysaccharide nano-selenium, artemisia annua extract, mugwort leaf extract, perilla extract, mulberry leaf extract, roxburghii extract and crab shell powder in 5-20 parts of water, stir thoroughly to dissolve, centrifuge and freeze-dry the supernatant to prepare an antibacterial composition.

6. Use of the antibacterial composition according to any one of claims 1 to 4 in the preparation of skin care products.

7. The use according to claim 6, characterized in that The skin care product is a skin care ointment.

8. The use according to claim 7, characterized in that The skin care ointment further comprises azone, ethyl hydroxybenzoate, anhydrous ethanol, carbomer, sodium hyaluronate, glycerin and triethanolamine.

9. The use according to claim 8, characterized in that The preparation method of the ointment comprises the following steps: (1) dissolving American ginseng seed polysaccharide nano-selenium, Artemisia annua extract, Artemisia argyi extract, Perilla frutescens extract, Mulberry leaf extract, Rosa roxburghii extract and crab shell powder in 5-20 parts of water, stirring and dissolving them thoroughly to obtain an antibacterial composition raw material liquid; (2) Add 2-5 parts of carbomer to 40-60 ml of water and allow to swell for 2-6 hours; prepare 2-5 parts of a 2-5 wt% sodium hyaluronate solution; (4) taking the swollen carbomer, sodium hyaluronate solution, and 0.2-1.0 parts of glycerol and stirring evenly to obtain a carbomer mixed solution; (5) Dissolve 0.5-1.0 parts of azone and 0.1-0.3 parts of ethylparaben in 2-3 parts of anhydrous ethanol, mix well, add to the carbomer mixed solution, then add 0.05-0.10 parts of triethanolamine and stir into a gel, finally add the prepared antibacterial composition raw material solution and mix well to obtain an antibacterial composition ointment.

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