Composition with exfoliating and whitening effects and application of composition in cosmetics

Abstract

The invention relates to a composition with exfoliating and whitening effects and application of the composition in cosmetics, and belongs to the technical field of cosmetics. The composition consists of the following components in parts by weight: 1-4 parts of lactobionic acid, 3-5 parts of a tea polysaccharide solution and 1-3 parts of kale leaf extract, the tea polysaccharide solution is prepared by fermenting bacillus subtilis, trametes hirsutum and tea, extracting and purifying. The content of tea polysaccharide in the tea polysaccharide solution is increased, the content of small molecular substances is increased, and permeation and absorption are facilitated. Meanwhile, the tea polysaccharide solution, lactobionic acid and kale leaf extract are combined for use, so that compared with other substances with similar effects, the combination effect is better, internal and external dual whitening and brightening can be realized, the effects are stably and lastingly played, the skin is not irritated, and the mask is fine and glossy.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a composition with exfoliating and whitening effects and application thereof in cosmetics. Background Art

[0002] When it comes to skincare, people often look to exfoliation and whitening to improve their skin. Removing dead skin cells not only facilitates the absorption of subsequent skincare products but also ensures longer-lasting whitening results. However, traditional exfoliants often contain highly irritating acids, such as salicylic acid, which can easily cause skin irritation. Tea polysaccharides, kale extract, and lactobionic acid offer a new solution for safely and effectively achieving these skincare goals.

[0003] Tea polysaccharides are acidic glycoproteins extracted from tea leaves. Composed of polysaccharides, proteins, pectin, and other substances, they possess numerous biological activities, including antioxidant, immunomodulatory, and hypoglycemic properties. In the cosmetics field, their antioxidant and moisturizing properties have attracted considerable attention. On the one hand, they effectively scavenge free radicals, reduce oxidative damage to the skin, and prevent the root cause of skin dullness, laying the foundation for whitening. On the other hand, they form a moisturizing film on the skin's surface, preventing water loss and maintaining hydration, creating optimal conditions for the absorption of subsequent whitening ingredients. However, the current extraction process for tea polysaccharides is complex and costly, and as large molecules, they have poor transdermal absorption. Optimizing their application remains a research topic.

[0004] Kale is rich in vitamins, minerals, dietary fiber, and bioactive ingredients like flavonoids and lutein, offering antioxidant, anti-inflammatory, and moisturizing properties. Its extracts can be used in acne-removing and antioxidant products, particularly the leaves, which are the primary site of photosynthesis. They are rich in nutrients, have a soft texture, and a loose cell structure, making them highly efficient for extraction. In skincare, kale extract can protect the skin from free radical damage, reduce inflammation, replenish nutrients and moisture, enhance skin health, and better enable the skin to recover and absorb whitening ingredients after exfoliation, contributing to a radiant complexion.

[0005] Lactobionic acid, a third-generation alpha-hydroxy acid (AHA), is produced by the oxidation of lactose and offers a gentle exfoliating effect. It promotes stratum corneum renewal and gently removes dead skin cells, leaving skin smooth and delicate. It is far less irritating than traditional acids, addressing the issue of skin sensitivity during exfoliation. Lactobionic acid also possesses antioxidant and moisturizing properties, scavenging free radicals and reducing oxidative damage while absorbing moisture to maintain skin's moisture levels. This ensures both skin whitening and overall skin health and hydration. Summary of the Invention

[0006] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a composition with exfoliating and whitening effects and its application in cosmetics.

[0007] To achieve the above object, the technical solution adopted by the present invention is:

[0008] In a first aspect, the present invention provides a composition with exfoliating and whitening effects, which is composed of the following ingredients in parts by weight: 1 to 4 parts of lactobionic acid, 3 to 5 parts of tea polysaccharide solution and 1 to 3 parts of kale leaf extract; the tea polysaccharide solution is obtained by fermenting tea with Bacillus subtilis and Trametes trichoderma and then extracting and purifying it.

[0009] The tea polysaccharide solution utilized in the present invention is tea polysaccharide extracted and purified after fermentation of tea with Bacillus subtilis and Trametes trichotillomanii. By optimizing the fermentation process, the tea polysaccharide is decomposed into small molecular substances, thereby solving the problem of poor permeability and absorption of the tea polysaccharide. Compared with the combination of other bacteria (such as the combination of Aspergillus niger or yeast and Trametes trichotillomanii), the fermentation combination of Bacillus subtilis and Trametes trichotillomanii has better effect.

[0010] The tea polysaccharide solution of the present invention has an increased tea polysaccharide content and a higher content of small molecules, which facilitates penetration and absorption. Furthermore, the present invention combines the tea polysaccharide solution with lactobionic acid and kale leaf extract. Compared to other substances with similar efficacy (such as salicylic acid and kale stem extract), the present combination has a better effect, achieving both instant and long-lasting whitening.

[0011] The kale leaf extract in the composition of this invention contains a large amount of natural pigments, which can reduce the use of pigments and reduce irritation. Care should be taken to protect the product from light during use. Lactic acid bacteria, due to their gentle exfoliating properties, combined with tea polysaccharides and kale, can achieve both internal and external whitening and brightening effects, achieving stable and long-lasting effects without irritating the skin while leaving it delicate and radiant.

[0012] As a preferred embodiment of the composition of the present invention, the composition is composed of the following components in parts by weight: 1-2 parts of lactobionic acid, 4-5 parts of tea polysaccharide solution, and 2-3 parts of kale leaf extract. Within this range, the composition has a better effect.

[0013] As a preferred embodiment of the composition of the present invention, the composition is composed of the following components in parts by weight: 2 parts of lactobionic acid, 5 parts of tea polysaccharide solution, and 2 parts of kale leaf extract. At this content, the composition has the best effect.

[0014] As a preferred embodiment of the composition of the present invention, the method for preparing the tea polysaccharide solution comprises the following steps:

[0015] S1. suspending activated Bacillus subtilis and Trametes trichomoniasis in culture medium to obtain Bacillus subtilis suspension and Trametes trichomoniasis suspension, respectively; the culture medium is composed of: 1.5-2.5 g glucose, 1-2 g microcrystalline cellulose, 7.5-8.5 g soybean meal hydrolyzate, 2-4 g yeast extract, 1-1.4 g dipotassium hydrogen phosphate, 0.2-0.6 g magnesium sulfate, 2-3 g calcium carbonate, 0.05-0.15 mg manganese sulfate, and the volume is adjusted to 1 L with water;

[0016] S2, mixing the prepared Bacillus subtilis suspension with green tea powder and fermenting for 24 to 48 hours; then adding the Trametes trichomoniasis suspension and continuing to ferment for 24 to 60 hours to obtain a fermentation product;

[0017] S3. The fermented product of step S2 is mixed with water, extracted, filtered, and the filtrate is concentrated to obtain a concentrated solution I. The concentrated solution I is added with an alcohol aqueous solution, fully mixed, allowed to stand, centrifuged, and a precipitate is collected. The precipitate is dissolved in deionized water to obtain a dissolved solution. The dissolved solution is concentrated to obtain a concentrated solution II. The concentrated solution II is ultrafiltered with a membrane pore size of 10 to 25 kDa. The permeate obtained is the tea polysaccharide solution.

[0018] In the preparation method of the tea polysaccharide solution of the present invention, fermenting Bacillus subtilis and Trametes trichomoniasis successively can achieve better fermentation effect.

[0019] As a preferred embodiment of the composition of the present invention, in step S1, the viable cell count of the Bacillus subtilis suspension is 1×10 7 ~8.5×10 7 CFU / L, the viable bacterial count of the Trametes fungus suspension is 2×10 6 ~7×10 6 CFU / L;

[0020] As a preferred embodiment of the composition of the present invention, in step S1, the viable cell count of the Bacillus subtilis suspension is 5×10 7 CFU / L, the viable bacterial count of the Trametes fungus suspension is 5×10 6 CFU / L; the composition of the culture medium is: 2g glucose, 1.5g microcrystalline cellulose, 8g soybean meal hydrolyzate, 3g yeast extract, 1.2g dipotassium hydrogen phosphate, 0.4g magnesium sulfate, 2.5g calcium carbonate, 0.1mg manganese sulfate, and the volume is adjusted to 1L with water.

[0021] As a preferred embodiment of the composition of the present invention, in step S2, the particle size of the green tea powder is 60-80 mesh; preferably, the particle size of the green tea powder is 70 mesh. When the particle size is greater than 80 mesh, cellulose (a substrate for Trametes trichomoniasis) and tea polysaccharide precursors are mainly provided, resulting in an insufficient tea polysaccharide concentration (less than 80%).

[0022] As a preferred embodiment of the composition of the present invention, in step S2, the mass ratio of the Bacillus subtilis suspension to the green tea powder is (0.1-1):100, the fermentation temperature is 20-42°C, and the mass ratio of the Trametes trichomoniasis suspension to the green tea powder is (0.5-2):100.

[0023] As a preferred embodiment of the composition of the present invention, in step S2, the mass ratio of the Bacillus subtilis suspension to green tea powder is 0.5:100; the mass ratio of the Trametes trichotillomanii suspension to green tea powder is 1:100; the fermentation temperature is 30°C, the fermentation time of Bacillus subtilis is 24 hours, and the fermentation time of Trametes trichotillomanii is 60 hours. If the fermentation time of Bacillus subtilis or Trametes trichotillomanii is less than 24 hours, the purity of tea polysaccharides will be less than 80%.

[0024] As a preferred embodiment of the composition of the present invention, in step S3, the mass ratio of the fermentation product to water is 1:(5-80); the extraction temperature is 60-98°C, and the extraction time is 25-240 min; the volume ratio of concentrated solution I to alcohol aqueous solution is 1:(2-6); the volume concentration of alcohol in the alcohol aqueous solution is 60-95%; the filtrate is concentrated to 1 / 10-1 / 2 of the original volume to obtain concentrated solution I; the dissolved solution is concentrated to 1 / 10-1 / 2 of the original volume to obtain concentrated solution II; the ratio of the precipitate to deionized water is 1 g:(4-5) mL.

[0025] As a preferred embodiment of the composition of the present invention, in step S3, the mass ratio of the fermentation product to water is 1:10; the extraction temperature is 80°C, and the extraction time is 180 min; the filtration is performed using a microfiltration membrane; the filtrate is concentrated to 1 / 5 of the original volume to obtain concentrated solution I; the volume concentration of the alcohol-water solution is 95%; the volume ratio of the concentrated solution I to the alcohol-water solution is 1:4; the ratio of the precipitate to deionized water is 1 g:5 mL; the dissolved solution is concentrated to 1 / 5 of the original volume to obtain concentrated solution II; and the pore size of the ultrafiltration membrane is 20-25 KDa.

[0026] In a second aspect, the present invention provides use of the above composition having exfoliating and whitening effects in the preparation of cosmetics.

[0027] As a preferred embodiment of the application of the present invention, the cosmetics include essence products, creams, sunscreens, facial masks, makeup, cleansing products, etc.; the essence products include essence liquid.

[0028] In a third aspect, the present invention provides a cosmetic comprising the above composition.

[0029] As a preferred embodiment of the cosmetic of the present invention, the cosmetic further comprises auxiliary materials.

[0030] In a fourth aspect, the present invention provides an essence comprising, by weight percentage, 5-12% of the above composition, 15.6-26.1% of auxiliary materials and the balance water.

[0031] As a preferred embodiment of the essence of the present invention, the essence comprises, by weight percentage, 8-10% of the above composition, 18-21.5% of auxiliary materials and the balance water.

[0032] As a preferred embodiment of the essence of the present invention, the auxiliary materials include one or more of a moisturizer, a solvent, a skin conditioner, an emulsifier, a chelating agent or a preservative.

[0033] As a preferred embodiment of the essence of the present invention, the moisturizer includes one or both of glycerol and glyceryl polyether-26; the solvent includes one or both of butylene glycol and propylene glycol; the skin conditioning agent includes one or both of polydimethylsiloxane and isononyl isononanoate; the emulsifier includes one or both of PEG-40 hydrogenated castor oil and polysorbates; the chelating agent includes one or more of disodium ethylenediaminetetraacetic acid (EDTA-2Na) or citric acid and its salts; and the preservative includes one or more of phenoxyethanol, hydroxyacetophenone, methylparaben or chlorphenesin.

[0034] As a preferred embodiment of the essence of the present invention, the essence includes, by weight percentage, 5-12% of the above composition, 5-8% of a moisturizer, 3-6% of a solvent, 5-7% of a skin conditioner, 2-4% of an emulsifier, 0.1-0.3% of a chelating agent, 0.5-0.8% of a preservative, and the remainder being water.

[0035] As a preferred embodiment of the essence of the present invention, the essence includes, by weight percentage, 1-4% lactobionic acid, 3-5% tea polysaccharide solution, 1-3% kale leaf extract, 4-5% glycerol, 1-3% glycerol polyether-26, 3-6% butylene glycol, 3-4% polydimethylsiloxy, 2-3% isononyl isononanoate, 2-4% PEG-40 hydrogenated castor oil, 0.1-0.3% disodium edetate, 0.5-0.8% phenoxyethanol, and the balance water.

[0036] As a preferred embodiment of the essence of the present invention, the essence includes, by weight percentage, 2% lactobionic acid, 5% tea polysaccharide solution, 2% kale leaf extract, 5% glycerol, glyceryl polyether-261%, 6% butylene glycol, 3% polydimethylsiloxane, 2% isononyl isononanoate, 2% PEG-40 hydrogenated castor oil, 0.1% disodium edetate, 0.5% phenoxyethanol and the balance water.

[0037] In a fifth aspect, the present invention provides a method for preparing the essence, comprising the following steps:

[0038] S1. Heat water to 60-70°C, add a moisturizer and a solvent and fully dissolve them, then add a chelating agent and fully dissolve them to obtain an aqueous phase mixture;

[0039] S2. Mix the skin conditioner evenly and heat to 60-70° C., then add the emulsifier and fully dissolve it to obtain an oil phase mixture;

[0040] S3, evenly dispersing the oil phase mixture in the water phase mixture, cooling to 40-45°C, adding a preservative and mixing, and homogenizing to obtain an oil-water mixture;

[0041] S4. Cool the oil-water mixture to 35-40° C., add the composition, fully dissolve and disperse it, and adjust the pH value to 5-6.5 to obtain the essence.

[0042] As a preferred embodiment of the method for preparing the essence of the present invention, in step S1, the heating is heating to 70°C.

[0043] As a preferred embodiment of the method for preparing the essence of the present invention, in step S2, the heating is heating to 70°C.

[0044] In a preferred embodiment of the method for preparing the essence of the present invention, in step S3, the temperature is lowered to 45°C; the homogenization speed is 4000-6000 rpm, and the homogenization time is 3-5 minutes to ensure uniform dispersion of the ingredients. After homogenization, stirring is continued at 300-500 rpm and cooling is performed.

[0045] As a preferred embodiment of the method for preparing the essence of the present invention, in step S4, the cooling is cooling to 35°C.

[0046] Compared with the prior art, the present invention has the following beneficial effects:

[0047] The tea polysaccharide solution of the present invention utilizes Bacillus subtilis and Trametes trichomoniasis to ferment tea. By optimizing the fermentation process, the tea polysaccharides are decomposed into small molecular substances, solving the problem of poor penetration and absorption of tea polysaccharides. Compared with the combination of other bacteria, the fermentation using the combination of Bacillus subtilis and Trametes trichomoniasis has a better effect. The tea polysaccharide content in the tea polysaccharide solution of the present invention is improved, and the content of small molecular substances is increased, which is conducive to penetration and absorption. At the same time, the present invention combines the tea polysaccharide solution with lactobionic acid and kale leaf extract. Compared with other substances with similar efficacy, the combination of the present invention has a better effect and can achieve instant whitening and long-lasting whitening. Lactic acid bacteria have the effect of gently exfoliating keratin. Combined with tea polysaccharides and kale, it can achieve dual whitening and brightening inside and outside, stably and long-lastingly exerting efficacy, and does not irritate the skin while making it delicate and shiny. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] Figure 1 This is a curve diagram of the change in moisture retention rate in Test Example 3 of the present invention. DETAILED DESCRIPTION

[0049] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0050] Unless otherwise specified, other materials and reagents used in the examples can be obtained from commercial sources.

[0051] Lactobionic acid, white powder, was purchased from Guangzhou Tinglan Biotechnology Co., Ltd.

[0052] Kale leaf extract was purchased from Guangzhou Hengyue Biotechnology Co., Ltd.

[0053] The composition of the culture medium is: 2 g glucose, 1.5 g microcrystalline cellulose, 8 g soybean meal hydrolyzate, 3 g yeast extract, 1.2 g dipotassium hydrogen phosphate, 0.4 g magnesium sulfate, 2.5 g calcium carbonate, 0.1 mg manganese sulfate, and the volume is adjusted to 1 L with water.

[0054] HSF cell culture medium: purchased from Shanghai Yaji Biotechnology Co., Ltd.

[0055] Example 1

[0056] A composition comprising the following components in parts by weight: 5 parts of tea polysaccharide solution, 2 parts of lactobionic acid, and 2 parts of kale leaf extract; a method for preparing the tea polysaccharide solution comprises the following steps:

[0057] S1. Activated Bacillus subtilis and Trametes trichomoniasis were suspended in the culture medium respectively to obtain a viable bacterial count of 5×10 7 CFU / L of Bacillus subtilis suspension, with a viable count of 5×10 6 CFU / L of Trametes fungus suspension;

[0058] S2. The prepared Bacillus subtilis suspension was first mixed with green tea powder, wherein the particle size of the green tea powder was 70 mesh and the mass ratio of the Bacillus subtilis suspension to the green tea powder was 0.5:100, and the mixture was fermented at 30°C for 24 h. Then, a Trametes trichomoniasis suspension was added, wherein the mass ratio of the Trametes trichomoniasis suspension to the green tea powder was 1:100, and the mixture was fermented at 30°C for 60 h to obtain a fermentation product;

[0059] S3. The fermented product is added to water at a mass ratio of 1:10, extracted at 80°C for 180min, filtered through a microfiltration membrane, and the filtrate is concentrated to 1 / 5 of the original volume to obtain a concentrated solution I. The concentrated solution I is added with an alcohol aqueous solution having a volume concentration of 95%, and the volume ratio of the concentrated solution I to the alcohol aqueous solution is 1:4. The mixture is thoroughly mixed, allowed to stand, and centrifuged. The precipitate is collected and dissolved in deionized water to obtain a dissolved solution in which the ratio of the precipitate to deionized water is 1g:5mL. The dissolved solution is concentrated to 1 / 5 of the original volume to obtain a concentrated solution II. The concentrated solution II is ultrafiltered with an ultrafiltration membrane pore size of 20-25KDa, and the permeate obtained is the tea polysaccharide solution.

[0060] Example 2

[0061] A composition, which is the same as Example 1, differs only in the preparation method of the tea polysaccharide solution. The preparation method of this embodiment comprises the following steps:

[0062] S1. Activated Bacillus subtilis and Trametes trichomoniasis were suspended in the culture medium respectively to obtain a viable bacterial count of 1×10 7 CFU / L of Bacillus subtilis suspension, with a viable count of 2×10 6 CFU / L of Trametes fungus suspension;

[0063] S2. The prepared Bacillus subtilis suspension was first mixed with green tea powder, wherein the particle size of the green tea powder was 60 mesh and the mass ratio of the Bacillus subtilis suspension to the green tea powder was 1:100. The mixture was fermented at 20° C. for 48 h. Then, a Trametes trichomoniasis suspension was added, wherein the mass ratio of the Trametes trichomoniasis suspension to the green tea powder was 2:100. The mixture was fermented at 20° C. for 60 h to obtain a fermentation product.

[0064] S3. The fermented product is added to water at a mass ratio of 1:5, extracted at 60°C for 240min, filtered through a microfiltration membrane, and the filtrate is concentrated to 1 / 2 of the original volume to obtain a concentrated solution I. The concentrated solution I is added with an alcohol aqueous solution having a volume concentration of 60%, and the volume ratio of the concentrated solution I to the alcohol aqueous solution is 1:2. The mixture is thoroughly mixed, allowed to stand, and centrifuged. The precipitate is collected and dissolved in deionized water to obtain a dissolved solution. The ratio of the precipitate to deionized water is 1g:4mL. The dissolved solution is reduced to 1 / 2 of the original volume to obtain a concentrated solution II. The concentrated solution II is ultrafiltered with an ultrafiltration membrane pore size of 10 to 20KDa. The permeate obtained is the tea polysaccharide solution.

[0065] Example 3

[0066] A composition, which is the same as Example 1, differs only in the preparation method of the tea polysaccharide solution. The preparation method of this embodiment comprises the following steps:

[0067] S1. Activated Bacillus subtilis and Trametes trichomoniasis were suspended in the culture medium respectively, and the number of viable bacteria was 8.5×10 7 CFU / L of Bacillus subtilis suspension, with a viable count of 7×10 6 CFU / L of Trametes fungus suspension;

[0068] S2. The prepared Bacillus subtilis suspension was first mixed with green tea powder, wherein the particle size of the green tea powder was 80 mesh and the mass ratio of the Bacillus subtilis suspension to the green tea powder was 0.1:100. The mixture was fermented at 42° C. for 24 h. Then, a Trametes trichomoniasis suspension was added, wherein the mass ratio of the Trametes trichomoniasis suspension to the green tea powder was 0.5:100. The mixture was fermented at 42° C. for 24 h to obtain a fermentation product.

[0069] S3. The fermented product is added to water at a mass ratio of 1:80, extracted at 98° C. for 25 min, filtered through a microfiltration membrane, and the filtrate is concentrated to 1 / 10 of the original volume to obtain a concentrated solution I. The concentrated solution I is added with an alcohol aqueous solution having a volume concentration of 95%, and the volume ratio of the concentrated solution I to the alcohol aqueous solution is 1:6. The mixture is thoroughly mixed, allowed to stand, and centrifuged. The precipitate is collected and dissolved in deionized water to obtain a dissolved solution in which the ratio of the precipitate to deionized water is 1 g:5 mL. The dissolved solution is concentrated to 1 / 10 of the original volume to obtain a concentrated solution II. The concentrated solution II is ultrafiltered with an ultrafiltration membrane pore size of 10 to 25 KDa, and the permeate obtained is the tea polysaccharide solution.

[0070] Comparative Example 1

[0071] A composition is the same as Example 1, except that the preparation method of the tea polysaccharide solution is different (different bacteria and different fermentation sequence). The preparation method of this comparative example comprises the following steps:

[0072] S1. Activated Aspergillus niger and Trametes trichoderma were suspended in the culture medium respectively to obtain a viable bacterial count of 5×10 7 CFU / L of Aspergillus niger suspension, with a viable count of 5×10 6 CFU / L of Trametes fungus suspension;

[0073] S2. The prepared Aspergillus niger suspension and Trametes trichotillomanii suspension were simultaneously mixed with green tea powder, wherein the particle size of the green tea powder was 70 mesh, the mass ratio of the Aspergillus niger suspension to the green tea powder was 0.5:100, and the mass ratio of the Trametes trichotillomanii suspension to the green tea powder was 1:100, and the mixture was fermented at 30° C. for 84 h to obtain a fermentation product;

[0074] S3. Same as Example 1.

[0075] Comparative Example 2

[0076] A composition is the same as Example 1, except that the preparation method of the tea polysaccharide solution is different (the fermentation sequence is different). The preparation method of this comparative example comprises the following steps:

[0077] S1, same as Example 1;

[0078] S2. The prepared Bacillus subtilis suspension and Trametes trichotillomanii suspension were simultaneously mixed with green tea powder, wherein the particle size of the green tea powder was 70 mesh, the mass ratio of the Bacillus subtilis suspension to the green tea powder was 0.5:100, and the mass ratio of the Trametes trichotillomanii suspension to the green tea powder was 1:100, and the mixture was fermented at 30° C. for 84 h to obtain a fermentation product;

[0079] S3. Same as Example 1.

[0080] Comparative Example 3

[0081] A composition is the same as Example 1, except that the preparation method of the tea polysaccharide solution is different (different bacteria). The preparation method of this comparative example only replaces Bacillus subtilis in steps S1 and S2 with yeast, and the rest is the same as Example 1.

[0082] Comparative Example 4

[0083] A composition is the same as Example 1, except that the preparation method of the tea polysaccharide solution is different (without fermentation). The preparation method of this comparative example comprises the following steps:

[0084] Green tea powder is added to water with a mass ratio of green tea powder to water of 1:10, extracted at 80°C for 180 minutes, filtered through a microfiltration membrane, and the filtrate is concentrated to 1 / 5 of the original volume to obtain a concentrated solution I. The concentrated solution I is added to an alcohol aqueous solution with a volume concentration of 95% in a volume ratio of the concentrated solution I to the alcohol aqueous solution of 1:4. The mixture is thoroughly mixed, allowed to stand, and centrifuged. The precipitate is collected and dissolved in deionized water to obtain a dissolved solution with a ratio of the precipitate to deionized water of 1 g:5 mL. The dissolved solution is concentrated to 1 / 5 of the original volume to obtain a concentrated solution II. The concentrated solution II is ultrafiltered with an ultrafiltration membrane having a pore size of 20 to 25 kDa, and the permeate obtained is the tea polysaccharide solution.

[0085] Comparative Example 5

[0086] A composition is the same as Example 1, except that the preparation method of the tea polysaccharide solution is different (different ultrafiltration particle size). The preparation method of this comparative example only replaces the pore size of the ultrafiltration membrane in step S3 with 5-9KDa, and the rest is the same as Example 1.

[0087] Comparative Example 6

[0088] A composition, same as Example 1, the only difference is that the preparation method of tea polysaccharide solution is different (the particle size of ultrafiltration is different), the preparation method of this comparative example only replaces the pore size of ultrafiltration membrane in step S3 to 30-50KDa, and the rest is the same as Example 1.

[0089] Comparative Example 7

[0090] A composition, same as Example 1, the only difference is that the preparation method of tea polysaccharide solution is different (the fermentation time is different), the preparation method of this comparative example only replaces the fermentation time of Bacillus subtilis in step S2 to 60h, and the fermentation time of Trametes versicolor to 20h.

[0091] Test Example 1

[0092] To determine the effect of the preparation method of tea polysaccharide solution on the molecular weight, a third-party testing agency was commissioned to conduct testing, referring to GB / T 27843-2011 "Chemicals Determination of Low Molecular Weight Components of Polymers Gel Permeation Chromatography (GPC)", the tea polysaccharide solution in Example 1-3 and Comparative Example 1-7 was placed in a gel permeation chromatograph (instrument model: Agilent 1260InfinityII) for testing, mobile phase: 0.1M NaNO3, detector: RID, chromatographic column: Waters Ultrahydrogel 1000 and Waters Ultrahydrogel 120 in series, temperature: 40℃, flow rate: 0.8ml / min, standard: polyethylene oxide / polyethylene glycol PEO / PEG, sample volume: 100μl.

[0093] The molecular weight distribution of tea polysaccharide solution prepared by different preparation methods is shown in Table 1 below,

[0094] Table 1

[0095]

[0096]

[0097] The results in Table 1 show that the tea polysaccharide solutions prepared in Examples 1-3 contain more substances with a molecular weight less than 10KDa, and substances with a molecular weight of less than 10KDa account for more than 80%. This indicates that the tea polysaccharide solutions prepared by the method of the embodiments of the present invention contain more small-molecule substances. The tea polysaccharide solutions prepared in Comparative Examples 1-5 and 7 contain more substances with a molecular weight greater than 10KDa, and substances with a molecular weight of less than 10KDa account for less than 49.5%; the tea polysaccharide solution prepared in Comparative Example 6 contains more substances with a molecular weight less than 10KDa, but substances with a molecular weight of less than 10KDa account for less than 72%, and the content of small-molecule substances is significantly lower than the preparation method of the tea polysaccharide solution in the embodiment. This indicates that the preparation method of the tea polysaccharide solution in the embodiment can prepare a tea polysaccharide solution with a higher content of small-molecule substances.

[0098] Test Example 2

[0099] The effects of different preparation methods on the safety of tea polysaccharide solutions were tested. The safety of tea polysaccharide solutions was tested by testing the cytotoxicity of different tea polysaccharide solutions. The method is as follows:

[0100] Human skin fibroblasts (HSF cells) were used for cytotoxicity testing. The tea polysaccharide solutions in Examples 1-3 and Comparative Examples 1-7 were used as experimental group samples, while the blank control group contained only deionized water without any substance added.

[0101] HFB cells were cultured at a rate of 1.0 × 10 5 100 μL of the solution was seeded into 96-well cell culture plates, with 6,000 cells per well, and cultured in a 37°C cell culture incubator for 24 hours. After HFB cells in the logarithmic growth phase adhered to the cell wall, the corresponding drugs were administered. A blank control group was treated with cell-free blank culture medium, and a negative control group was treated with the same volume of culture medium. Cultures were continued at 37°C for 24 hours before termination. The drug-treated culture medium was removed, and the cells were washed twice with PBS. Cells were then fixed with 4% paraformaldehyde for 5 minutes. DAPI staining solution was diluted to a final concentration of 50 μmol / L and 100 μL was added to each well. The cells were stained for 20 minutes at room temperature in the dark. After washing with PBS, cell morphology was observed under a fluorescence microscope. A 10 μM EDU working solution was added for 2 hours. EDU reaction and staining procedures were performed according to the EDU cell proliferation detection kit instructions. Cells were then observed and photographed under a fluorescence microscope. Fluorescence images were quantified using Image-Pro Plus 6.0.

[0102] The fluorescence density results corresponding to the tea polysaccharide solutions prepared by different preparation methods are shown in Table 2 below.

[0103] Table 2

[0104] Test samples EDUIntensity% Test samples EDU Intensity % Blank control 9.3 Comparative Example 3 22.4 Example 1 46.7 Comparative Example 4 15.2 Example 2 41.2 Comparative Example 5 27.8 Example 3 43.5 Comparative Example 6 29.3 Comparative Example 1 23.2 Comparative Example 7 21.6 Comparative Example 2 25.6 / /

[0105] The results of Table 2 show that the fluorescence density corresponding to the tea polysaccharide solutions of Examples 1-3 is 41% to 47%. The fluorescence density corresponding to the tea polysaccharide solutions of Comparative Examples is 15.2% to 29.3%, which is significantly lower than that of Examples. The cells cultured with the tea polysaccharide solutions of Examples proliferate more actively, indicating that the tea polysaccharide solutions of Examples are safer for cells.

[0106] Test Example 3

[0107] Tea polysaccharides belong to polysaccharides, and the higher the content, the better the moisturizing effect. The influence of different preparation methods on the moisturizing effect of tea polysaccharide solutions was tested.

[0108] 1. Purpose and principle of test

[0109] The hydration of the skin is of great importance to maintain the epidermal barrier function, the activity of various enzymes, and the differentiation and desquamation of skin tissues, and is related to the health of the skin. The local use of moisturizing products is the main method for maintaining skin moisture and improving adverse symptoms.

[0110] An ideal moisturizing product should have a sustained moisturizing ability. This test method monitors the weight change of the test sample continuously, and draws a moisture retention rate-time curve. According to the trend of the moisture retention rate curve, the moisturizing ability of the test sample is observed, and the moisturizing effect of the test sample on the skin is evaluated. This test method is an in vitro method, which is suitable for water-based products.

[0111] The higher the moisture retention rate value, the stronger the moisturizing effect of the sample.

[0112] The formula for calculating the moisture retention rate is:

[0113]

[0114] 2. Experimental method

[0115] The tea polysaccharide solutions in Examples 1-3 and Comparative Examples 1-7 were used as experimental group samples, and 5% glycerol was used as a control group sample.

[0116] First, weigh each sample and record the data W0; then place the experimental group samples and the control group sample in a constant temperature and humidity device at 20°C and 40% humidity, and weigh the sample weight W t at 1h and 24h, calculate the moisture retention rate, and draw a moisture retention rate-time curve.

[0117] The moisture retention rate effect of tea polysaccharide solutions prepared by different preparation methods is shown in Table 3, and the moisture retention rate-time curve is shown in Figure 1

[0118] Table 3 ​

[0119]

[0120] Table 3 and Figure 1 The results show that the moisturizing rates of the tea polysaccharide solutions prepared in Examples 1-3 are significantly higher than those in Comparative Examples 1-7. The only difference between Comparative Example 1 and Example 1 is that the strains used for fermentation in the preparation method of the tea polysaccharide solution are different, and the order of strain fermentation is different; the only difference between Comparative Example 2 and Example 1 is that the order of strain fermentation in the preparation method of the tea polysaccharide solution is different; the only difference between Comparative Example 3 and Example 1 is that the bacteria used for fermentation in the preparation method of the tea polysaccharide solution are different; the corresponding moisturizing rates of the tea polysaccharide solutions are reduced, indicating that the strains used for fermentation and the order of strain fermentation will affect the performance of the tea polysaccharide solution, and the strains used for fermentation and the order of strain fermentation in the examples are more conducive to improving the moisturizing effect of tea polysaccharides. The only difference between Comparative Example 4 and Example 1 is that the green tea powder is not fermented in the preparation method of the tea polysaccharide solution, which means that fermentation is more conducive to improving the moisturizing effect of tea polysaccharides; the only difference between Comparative Examples 5 and 6 and Example 1 is that the particle size of the ultrafiltration membrane in the purification step in the preparation method of the tea polysaccharide solution is different, which means that the particle size of the ultrafiltration membrane will also affect the performance of the prepared tea polysaccharide solution, and the particle size of the ultrafiltration membrane in the embodiment is more conducive to improving the moisturizing effect of tea polysaccharides; the only difference between Comparative Example 7 and Example 1 is that the fermentation time in the preparation method of the tea polysaccharide solution is different, which means that the fermentation time will also affect the performance of the tea polysaccharide solution, and the fermentation time in the embodiment is more conducive to improving the moisturizing effect of tea polysaccharides.

[0121] Examples 4-6 and Comparative Examples 8-12

[0122] The components of the compositions of Examples 4-6 and Comparative Examples 8-12 are shown in Table 4 below. The preparation methods of the tea polysaccharide solutions are the same as those of Example 1.

[0123] Table 4

[0124]

[0125]

[0126] Application Examples

[0127] A serum comprising, by weight percentage, 5-12% of the above composition, 15.6-26.1% of excipients, and the balance water. The excipients include one or more of a moisturizer, a solvent, a skin conditioner, an emulsifier, a chelating agent, or a preservative. The moisturizer includes one or both of glycerol and glyceryl polyether-26; the solvent includes one or both of butylene glycol and propylene glycol; the skin conditioner includes one or both of polydimethylsiloxane and isononyl isononanoate; the emulsifier includes one or both of PEG-40 hydrogenated castor oil and polysorbates; the chelating agent includes one or more of disodium ethylenediaminetetraacetic acid (EDTA-2Na) or citric acid and its salts; and the preservative includes one or more of phenoxyethanol, hydroxyacetophenone, methylparaben, or chlorphenesin.

[0128] The compositions of Essences 1-9 and Comparative Essences 1-5 are shown in Tables 5 and 6 below, wherein the active ingredients in Essences 1-6 correspond to the compositions of Examples 1-6, respectively, and the active ingredients in Comparative Essences 1-5 correspond to the compositions of Comparative Examples 8-12, respectively.

[0129] Table 5

[0130]

[0131] Table 6

[0132]

[0133] The preparation method of the essence 1-7 and the comparative essence 1-5 comprises the following steps:

[0134] S1. While stirring, add deionized water to a stirring tank and heat to 70°C. Slowly add glycerol, glycereth-26, and butylene glycol and stir to ensure complete dissolution. Then add EDTA-2Na and stir to ensure dissolution to obtain an aqueous phase mixture.

[0135] S2. Mix polydimethylsiloxane and isononyl isononanoate evenly, heat to 70°C, then add PEG-40 hydrogenated castor oil, stir evenly to ensure that the emulsifier is completely dissolved, and obtain an oil phase mixture.

[0136] S3. Slowly add the oil phase mixture to the water phase mixture, stirring at a medium speed (about 300 rpm) while adding to ensure that the oil phase is evenly dispersed in the water phase. Continue stirring and slowly cool to 45°C, add the preservative (phenoxyethanol), and stir evenly. Set the homogenization speed to 4000 rpm and the homogenization time to 5 minutes to ensure that the ingredients are evenly dispersed. After homogenization is completed, continue stirring at a medium speed (about 300 rpm) to obtain an oil-water mixture.

[0137] S4. Cool the oil-water mixture to 35°C and reduce the stirring speed to 200 rpm. While stirring, slowly add lactobionic acid, tea polysaccharide solution, and kale leaf extract in sequence to ensure complete dissolution and dispersion (stir for 3 to 5 minutes after each addition). Adjust the pH to 5 to 6.5 to obtain the essence.

[0138] The preparation method of the essence 8 and 9 comprises the following steps:

[0139] S1. While stirring, add deionized water to a stirring tank and heat to 60°C. Slowly add glycerol, glycereth-26, and butylene glycol and stir to ensure complete dissolution. Then add EDTA-2Na and stir to ensure dissolution to obtain an aqueous phase mixture.

[0140] S2. Mix polydimethylsiloxane and isononyl isononanoate evenly, heat to 60°C, then add PEG-40 hydrogenated castor oil and stir evenly to ensure that the emulsifier is completely dissolved to obtain an oil phase mixture.

[0141] S3. Slowly add the oil phase mixture to the water phase mixture, stirring at a medium speed (about 500 rpm) while adding to ensure that the oil phase is evenly dispersed in the water phase. Continue stirring and slowly cool to 40°C, add the preservative (phenoxyethanol), and stir evenly. Set the homogenization speed to 6000 rpm and the homogenization time to 3 minutes to ensure that the ingredients are evenly dispersed. After homogenization is completed, continue stirring at a medium speed (about 500 rpm) to obtain an oil-water mixture.

[0142] S4. Cool the oil-water mixture to 40°C and reduce the stirring speed to 300 rpm. While stirring, slowly add lactobionic acid, tea polysaccharide solution, and kale leaf extract in sequence to ensure complete dissolution and dispersion (stir for 3 to 5 minutes after each addition). Adjust the pH to 5 to 6.5 to obtain the essence.

[0143] Test Example 4

[0144] Exfoliation and whitening tests were conducted using the aforementioned Essences 1-9 and Comparative Essences 1-5 as samples. A 2% niacinamide essence and a 1% salicylic acid essence were used as comparative samples. The only difference between these two essences and Essence 1 was that the active ingredient was replaced with 2% niacinamide or 1% salicylic acid. The specific testing methods are as follows:

[0145] 80 volunteers aged 20-45 years old, all of whom were female, were selected and divided into 16 groups, with 5 people in each group, who had the need for exfoliation and whitening.

[0146] Apply once every morning and evening after cleansing for 4 consecutive weeks; in the second and fourth weeks, survey patients on the effectiveness and irritation of the product using questionnaires.

[0147] The effectiveness evaluation criteria are shown in Table 7 below, and the irritation evaluation criteria are shown in Table 8 below.

[0148] Table 7

[0149]

[0150] Table 8

[0151]

[0152]

[0153] The exfoliating, whitening and stimulating effects of different essences are shown in Table 9 below.

[0154] Table 9

[0155]

[0156] The results in Table 9 show that the essence prepared from the composition of the present invention is mild and non-irritating, with excellent exfoliation and whitening effects. Adjusting the composition significantly reduced the exfoliation and whitening effects, indicating that only the combination of tea polysaccharide solution, lactobionic acid, and kale leaf extract can achieve better results.

[0157] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition with exfoliating and whitening effects, characterized in that: The tea polysaccharide solution is prepared by extracting and purifying tea leaves after fermentation with Bacillus subtilis and Trametes trichomoniasis.

2. The composition according to claim 1, wherein The method for preparing the tea polysaccharide solution comprises the following steps: S1. suspending activated Bacillus subtilis and Trametes trichotillomanii in culture medium to obtain Bacillus subtilis suspension and Trametes trichotillomanii suspension, respectively; The culture solution is composed of: 1.5-2.5 g glucose, 1-2 g microcrystalline cellulose, 7.5-8.5 g soybean meal hydrolyzate, 2-4 g yeast extract, 1-1.4 g dipotassium hydrogen phosphate, 0.2-0.6 g magnesium sulfate, 2-3 g calcium carbonate, 0.05-0.15 mg manganese sulfate, and the volume is adjusted to 1 L with water; S2, mixing the prepared Bacillus subtilis suspension with green tea powder and fermenting for 24 to 48 hours; then adding the Trametes trichomoniasis suspension and continuing to ferment for 24 to 60 hours to obtain a fermentation product; S3. The fermented product of step S2 is mixed with water, extracted, filtered, and the filtrate is concentrated to obtain a concentrated solution I. The concentrated solution I is added with an alcohol aqueous solution, fully mixed, allowed to stand, centrifuged, and a precipitate is collected. The precipitate is dissolved in deionized water to obtain a dissolved solution. The dissolved solution is concentrated to obtain a concentrated solution II. The concentrated solution II is ultrafiltered with a membrane pore size of 10 to 25 kDa. The permeate obtained is the tea polysaccharide solution.

3. Use of the composition according to claim 1 or 2 in the preparation of cosmetics.

4. A cosmetic, characterized in that: The invention comprises the composition according to claim 1 or 2.

5. The cosmetic according to claim 4, wherein The cosmetic also includes auxiliary materials.

6. An essence, characterized in that: Calculated by weight percentage, the composition comprises 5-12% of the composition according to claim 1 or 2, 15.6-26.1% of auxiliary materials and the balance water.

7. The essence according to claim 6, characterized in that The excipients include one or more of a moisturizer, a solvent, a skin conditioner, an emulsifier, a chelating agent or a preservative.

8. The essence according to claim 7, characterized in that The moisturizing agent includes one or both of glycerol and glyceryl polyether-26; and / or, the solvent comprises one or both of butylene glycol and propylene glycol; and / or, the skin conditioning agent comprises one or both of polydimethylsiloxane and isononyl isononanoate; and / or, the emulsifier comprises one or both of PEG-40 hydrogenated castor oil or polysorbates; and / or, the chelating agent comprises one or more of disodium edetate or citric acid and salts thereof; And / or, the preservative includes one or more of phenoxyethanol, hydroxyacetophenone, methylparaben or chlorphenesin.

9. The essence according to claim 7, characterized in that Calculated by weight percentage, the essence comprises 5-12% of the composition of claim 1 or 2, 5-8% of a moisturizer, 3-6% of a solvent, 5-7% of a skin conditioner, 2-4% of an emulsifier, 0.1-0.3% of a chelating agent, 0.5-0.8% of a preservative, and the balance being water.

10. The method for preparing the essence according to claim 9, characterized in that: The following steps are involved: S1. Heat water to 60-70°C, add a moisturizer and a solvent and fully dissolve them, then add a chelating agent and fully dissolve them to obtain an aqueous phase mixture; S2. Mix the skin conditioner evenly and heat to 60-70° C., then add the emulsifier and fully dissolve it to obtain an oil phase mixture; S3, evenly dispersing the oil phase mixture in the water phase mixture, cooling to 40-45°C, adding a preservative and mixing, and homogenizing to obtain an oil-water mixture; S4. Cool the oil-water mixture to 35-40° C., add the composition, fully dissolve and disperse it, and adjust the pH value to 5-6.5 to obtain the essence.

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