Preparation method and application of purslane extract

Abstract

The invention relates to a preparation method and application of a portulaca oleracea extract, and belongs to the technical field of portulaca oleracea extracts.The preparation method comprises the following steps that 1, fresh portulaca oleracea is taken to be cleaned, subjected to heat treatment, smashed and screened, and portulaca oleracea powder is obtained; (2) adding distilled water into the purslane powder, performing ultrasonic treatment, adding cellulase and papain, adjusting the pH value, performing enzymolysis treatment, heating, and cooling to room temperature to obtain a suspension; and (3) adding mixed bacteria and the mixture into the suspension, and carrying out multiple fermentation to obtain the purslane extract. According to the invention, mixed bacteria are adopted to improve the fermentation capability of purslane components, microbial cell lysates obtained after fermentation contain rich metabolites such as carbohydrates, ketones, organic acids and phenols, the oxidation resistance of the purslane extract is greatly improved, and the purslane extract can be used for improving the oxidation resistance of the purslane by inhibiting tyrosinase and DPPH free radicals of human skin. Therefore, the effects of beautifying the skin, whitening and resisting aging are achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of purslane extracts and relates to a preparation method of the purslane extract and an application thereof. Background Art

[0002] In recent years, a large number of plant extracts have been incorporated into the development of skincare products, including boletus whole plant extracts, orchid plant extracts, plum plant extracts, soybean plant extracts, and camellia plant extracts. Phytochemicals in natural skincare products include fatty acids (skin moisturizing), polyphenols (antioxidant, antibacterial, and anti-inflammatory), carotenoids (photoprotective), phytol (skin repairing), and vitamins (antioxidant, anti-aging). Therefore, extracting and enhancing the utilization of phytochemicals is becoming a new trend in their application in the food, pharmaceutical, and cosmetic sectors, necessitating the development of novel processing technologies to enhance the extraction efficiency of phytochemicals.

[0003] Traditional phytochemical extraction involves solvent extraction using organic reagents as a medium. This method optimizes the type and ratio of organic reagents to increase the solubility of phytochemicals. However, this process primarily targets free active compounds and can result in a reduced abundance of active compounds.

[0004] Probiotic products are also attracting significant attention, with many microorganisms being used in food, pharmaceuticals, cosmetics, and other fields. Probiotic communities, in particular, are enhanced by adding non-biological microbial preparations, also known as "cell lysates." The use of microbial metabolism to prepare plant fermentation extracts allows for the combination of plant extracts and microbial products, further enhancing product functionality. Summary of the Invention

[0005] The purpose of the present invention is to provide a preparation method and application of purslane extract. By using mixed bacteria to improve the fermentation ability of purslane components, the microbial cell lysate obtained after fermentation is rich in metabolic products such as carbohydrates, ketones, organic acids, phenols, etc., which greatly improves the antioxidant properties of purslane extract and achieves skin beautification, whitening and anti-aging effects by inhibiting tyrosinase and DPPH free radicals in human skin.

[0006] The purpose of the present invention can be achieved through the following technical solutions: A method for preparing a purslane extract, comprising the following steps: (1) Fresh purslane is washed, heat-treated, crushed, and sieved to obtain purslane powder; (2) adding distilled water to purslane powder, ultrasonically treating it, adding cellulase and papain, adjusting the pH value, performing enzymatic hydrolysis, heating it, and cooling it to room temperature to obtain a suspension; (3) The mixed bacteria and the mixture are added to the suspension and subjected to multiple fermentations to obtain a purslane extract.

[0007] As a preferred technical scheme of the present application, in step (1), the cleaning condition is cleaning 3-4 times with distilled water, the heat treatment condition is hot air drying at 60-65℃ for 24-30h, and the screening condition is screening through a 40 mesh sieve.

[0008] As a preferred technical scheme of the present application, in step (2), the ratio of the amount of the spilanthes acula powder to the amount of distilled water is 0.8-1g:30mL, the ultrasonic treatment condition is ultrasonic extraction at a power of 160-180W for 35-40min, the enzymatic treatment is enzymatic hydrolysis at pH 4.2-4.5 and a temperature of 48-50℃ for 45-50min, and the heating condition is heating at 100-105℃ for 5-10min; cellulase and papain can decompose the cellulose and protein of spilanthes acula, possibly increasing the available carbon source and protein peptides of the mixed bacteria and improving the growth rate and glycerol production capacity of the mixed bacteria.

[0009] As a preferred technical scheme of the present application, in step (2), the mass ratio of the spilanthes acula powder, cellulase and papain is 12-15:1.6-1.8:1.

[0010] As a preferred technical scheme of the present application, in step (3), the pH value of the suspension is 6.5-7.0, the mass ratio of the suspension, mixed bacteria and mixture is 500:0.8-1.0:0.1-0.15, and the fermentation time is 30-36h.

[0011] As a preferred technical scheme of the present application, in step (3), the mixture is a mixture of inorganic selenium and glucose in a mass ratio of 1:2.0-2.6, and the inorganic selenium is at least one of sodium selenate and sodium selenite; the addition of inorganic selenium makes selenium exist in the form of organic selenium after the mixed bacteria ferment the spilanthes acula, eliminating the toxicity of inorganic selenium and greatly improving the antioxidant property of the spilanthes acula extract.

[0012] As a preferred technical scheme of the present application, in step (3), the mixed bacteria are a mixture of candida glycerinogenes, saccharomyces boulardii and lactobacillus rhamnosus in a mass ratio of 0.1-0.2:0.5-0.8:1.

[0013] The present application discloses that the spilanthes acula extract is applied to a cosmetic emulsion, and the cosmetic emulsion comprises the following components in parts by weight: 0.02-0.03 parts of sodium hyaluronate, 0.1-0.2 parts of xanthan gum, 3-5 parts of water-soluble lanolin, 0.8-1.0 parts of hydrogenated castor oil, 0.5-1.5 parts of glycerol, 0.5-0.8 parts of spilanthes acula extract and 90-95 parts of deionized water.

[0014] Beneficial effects of the present invention: The present invention uses mixed bacteria to improve the fermentation ability of purslane components. The microbial cell lysate obtained after fermentation is rich in metabolic products such as carbohydrates, ketones, organic acids, phenols, etc., which greatly improves the antioxidant properties of the purslane extract and achieves skin beautification, whitening and anti-aging effects by inhibiting tyrosinase and DPPH free radicals in human skin. DETAILED DESCRIPTION

[0015] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined purpose of the invention, the specific implementation methods, structures, features and effects of the present invention are described in detail below in combination with the embodiments.

[0016] Example 1 A method for preparing a purslane extract, comprising the following steps: (1) Take fresh purslane and wash it with distilled water three times, dry it in hot air at 60℃ for 24 hours, grind it, and pass it through a 40-mesh sieve to obtain purslane powder; (2) Add distilled water to purslane powder, ultrasonically treat it, add cellulase and papain, adjust the pH value, perform enzymolysis, heat, and cool it to room temperature to obtain a suspension; wherein, the amount ratio of purslane powder to distilled water is 0.8 g:30 mL, the ultrasonic treatment condition is ultrasonic extraction at 160 W power for 35 min; the enzymolysis treatment is at pH 4.2 and temperature 48 ° C for 45 min; the heating condition is heating at 100 ° C for 5 min; the mass ratio of purslane powder, cellulase and papain is 12:1.6:1; (3) After adjusting the pH of the suspension to 6.5, the mixed bacteria and the mixture were added for multiple fermentation to obtain the purslane extract; wherein the mass ratio of the suspension, the mixed bacteria and the mixture was 500:0.8:0.1; the fermentation time was 30 h; the mixture was prepared by mixing sodium selenite and glucose in a mass ratio of 1:2.0; the mixed bacteria were prepared by mixing glycerol-producing Candida albicans, Saccharomyces boulardii and Lactobacillus rhamnosus in a mass ratio of 0.1:0.5:1.

[0017] The purslane extract is used in a cosmetic emulsion, which comprises the following components by weight: 0.02 parts of sodium hyaluronate, 0.1 parts of xanthan gum, 3 parts of water-soluble lanolin, 0.8 parts of hydrogenated castor oil, 0.5 parts of glycerin, 0.5 parts of purslane extract and 90 parts of deionized water.

[0018] Example 2 A method for preparing a purslane extract, comprising the following steps: (1) Take fresh purslane and wash it with distilled water three times, dry it in hot air at 62℃ for 26 hours, grind it, and pass it through a 40-mesh sieve to obtain purslane powder; (2) Add distilled water to purslane powder, ultrasonically treat it, add cellulase and papain, adjust the pH value, perform enzymolysis, heat, and cool it to room temperature to obtain a suspension; wherein, the amount ratio of purslane powder to distilled water is 0.85 g:30 mL, the ultrasonic treatment condition is ultrasonic extraction at 165 W power for 37 min; the enzymolysis treatment is at pH 4.3 and temperature 48.5 ° C for 46 min; the heating condition is heating at 102 ° C for 6 min; the mass ratio of purslane powder, cellulase and papain is 13:1.6:1; (3) After adjusting the pH of the suspension to 6.6, the mixed bacteria and the mixture were added for multiple fermentation to obtain the purslane extract; wherein the mass ratio of the suspension, the mixed bacteria and the mixture was 500:0.85:0.12; the fermentation time was 32 h; the mixture was prepared by mixing sodium selenite and glucose in a mass ratio of 1:2.2; the mixed bacteria were prepared by mixing glycerol-producing Candida albicans, Saccharomyces boulardii and Lactobacillus rhamnosus in a mass ratio of 0.12:0.6:1.

[0019] The purslane extract is used in a cosmetic emulsion, which comprises the following components by weight: 0.022 parts of sodium hyaluronate, 0.12 parts of xanthan gum, 3.5 parts of water-soluble lanolin, 0.82 parts of hydrogenated castor oil, 0.8 parts of glycerin, 0.7 parts of purslane extract and 92 parts of deionized water.

[0020] Example 3 A method for preparing a purslane extract, comprising the following steps: (1) Take fresh purslane and wash it with distilled water for 4 times, dry it in hot air at 64℃ for 28h, grind it, and pass it through a 40-mesh sieve to obtain purslane powder; (2) Add distilled water to purslane powder, ultrasonically treat it, add cellulase and papain, adjust the pH value, perform enzymolysis, heat, and cool it to room temperature to obtain a suspension; wherein, the amount ratio of purslane powder to distilled water is 0.9 g:30 mL, the ultrasonic treatment condition is ultrasonic extraction at 170 W power for 38 min; the enzymolysis treatment is at pH 4.4 and temperature 49 ° C for 48 min; the heating condition is heating at 103 ° C for 8 min; the mass ratio of purslane powder, cellulase and papain is 14:1.7:1; (3) After adjusting the pH of the suspension to 6.8, the mixed bacteria and the mixture were added for multiple fermentation to obtain the purslane extract; wherein the mass ratio of the suspension, the mixed bacteria and the mixture was 500:0.9:0.14; the fermentation time was 34 h; the mixture was prepared by mixing sodium selenite and glucose in a mass ratio of 1:2.5; the mixed bacteria were prepared by mixing glycerol-producing Candida albicans, Saccharomyces boulardii and Lactobacillus rhamnosus in a mass ratio of 0.18:0.7:1.

[0021] The purslane extract is used in a cosmetic emulsion, which includes the following components by weight: 0.028 parts of sodium hyaluronate, 0.18 parts of xanthan gum, 4.5 parts of water-soluble lanolin, 0.9 parts of hydrogenated castor oil, 1.3 parts of glycerin, 0.7 parts of purslane extract and 94 parts of deionized water.

[0022] Example 4 A method for preparing a purslane extract, comprising the following steps: (1) Take fresh purslane and wash it with distilled water for 4 times, dry it in hot air at 65℃ for 30h, grind it, and pass it through a 40-mesh sieve to obtain purslane powder; (2) Add distilled water to purslane powder, ultrasonically treat it, add cellulase and papain, adjust the pH value, perform enzymolysis, heat, and cool it to room temperature to obtain a suspension; wherein the amount ratio of purslane powder to distilled water is 1 g:30 mL, the ultrasonic treatment condition is ultrasonic extraction at 180 W power for 40 min; the enzymolysis treatment is at pH 4.5 and temperature 50°C for 50 min; the heating condition is heating at 105°C for 10 min; the mass ratio of purslane powder, cellulase and papain is 15:1.8:1; (3) After adjusting the pH of the suspension to 7.0, the mixed bacteria and the mixture were added and fermented multiple times to obtain the purslane extract; wherein the mass ratio of the suspension, the mixed bacteria and the mixture was 500:1.0:0.15; the fermentation time was 36 h; the mixture was prepared by mixing sodium selenite and glucose in a mass ratio of 1:2.6; the mixed bacteria were prepared by mixing glycerol-producing Candida albicans, Saccharomyces boulardii and Lactobacillus rhamnosus in a mass ratio of 0.2:0.8:1.

[0023] The purslane extract is used in a cosmetic emulsion, which comprises the following components by weight: 0.03 parts of sodium hyaluronate, 0.2 parts of xanthan gum, 5 parts of water-soluble lanolin, 1.0 parts of hydrogenated castor oil, 1.5 parts of glycerin, 0.8 parts of purslane extract and 95 parts of deionized water.

[0024] Comparative Example 1 Compared with Example 4, the difference is that no cellulase is used in Comparative Example 1, and the other components, preparation steps and parameters are the same.

[0025] Comparative Example 2 Compared with Example 4, the difference is that papain is not used in Comparative Example 2, and the other components, preparation steps and parameters are the same.

[0026] Comparative Example 3 Compared with Example 4, the difference is that Comparative Example 3 does not use glycerol-producing Candida, and the other components, preparation steps and parameters are the same.

[0027] Comparative Example 4 Compared with Example 4, the difference is that Comparative Example 4 does not use Saccharomyces boulardii, and the other components, preparation steps and parameters are the same.

[0028] Comparative Example 5 Compared with Example 4, the difference is that Lactobacillus rhamnosus is not used in Comparative Example 5, and the other components, preparation steps and parameters are the same.

[0029] Comparative Example 6 Compared with Example 4, the difference is that sodium selenite is not used in Comparative Example 6, and the other components, preparation steps and parameters are the same.

[0030] The purslane extracts prepared in Examples 1-4 and Comparative Examples 1-6 were subjected to tyrosinase inhibition tests and DPPH inhibition tests, respectively. The test results are shown in Table 1.

[0031] Table 1 From the test results in Table 1, it can be seen that compared with Comparative Examples 1-6, the purslane extracts prepared in Examples 1-4 of the present invention have significantly better inhibitory effects on tyrosinase and DPPH free radicals than Comparative Examples 1-6.

[0032] Thirty-five subjects were selected for a human skin patch test in accordance with the "Technical Specifications for Safety of Cosmetics (2015 Edition)." 0.02 ml of a stock solution of Portulaca oleracea extract was applied to the inner arm of the subjects using a standard patch tester and hypoallergenic tape for 24 hours. Changes in the skin condition at the test site were observed. No adverse reactions were observed at the test site after 24 hours. Therefore, the Portulaca oleracea extracts prepared in Examples 1-4 of the present invention are safe for human use and can be used without concern.

[0033] The above description is merely a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Although the present invention has been disclosed as above in terms of a preferred embodiment, it is not intended to limit the present invention. Any person skilled in the art can, without departing from the scope of the technical solution of the present invention, make some changes or modifications to equivalent embodiments using the technical contents disclosed above. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A method for preparing a purslane extract, characterized in that: The preparation method comprises the following steps: (1) Fresh purslane is washed, heat-treated, crushed, and sieved to obtain purslane powder; (2) adding distilled water to purslane powder, ultrasonically treating it, adding cellulase and papain, adjusting the pH value, performing enzymatic hydrolysis, heating it, and cooling it to room temperature to obtain a suspension; (3) The mixed bacteria and the mixture are added to the suspension and subjected to multiple fermentations to obtain a purslane extract.

2. The method for preparing a purslane extract according to claim 1, wherein: In step (1), the cleaning condition is to use distilled water to wash 3-4 times, the heat treatment condition is to dry with hot air at 60-65°C for 24-30h; and the screening condition is to pass through a 40-mesh sieve.

3. The method for preparing a Portulaca oleracea extract according to claim 1, wherein: In step (2), the ratio of the purslane powder to distilled water is 0.8-1 g:30 mL, the ultrasonic treatment condition is ultrasonic extraction at a power of 160-180 W for 35-40 min; the enzymatic hydrolysis treatment is at a pH of 4.2-4.5 and a temperature of 48-50° C. for 45-50 min; and the heating condition is heating at 100-105° C. for 5-10 min.

4. The method for preparing a Portulaca oleracea extract according to claim 1, wherein: In step (2), the mass ratio of the purslane powder, cellulase and papain is 12-15: 1.6-1.8：1。 5. The method for preparing the Portulaca oleracea extract according to claim 1, wherein: In step (3), the pH value of the suspension is 6.5-7.0; the mass ratio of the suspension, the mixed bacteria and the mixture is 500:0.8-1.0:0.1-0.15; and the fermentation time is 30-36 hours.

6. The method for preparing the Portulaca oleracea extract according to claim 1, wherein: In step (3), the mixture is prepared by mixing inorganic selenium and glucose in a mass ratio of 1:2.0-2.

6.

7. The method for preparing the Portulaca oleracea extract according to claim 1, wherein: In step (3), the mixed bacteria are prepared by mixing glycerol-producing Candida albicans, Saccharomyces boulardii, and Lactobacillus rhamnosus in a mass ratio of 0.1-0.2:0.5-0.8:

1.

8. A Portulaca oleracea extract prepared according to the preparation method according to any one of claims 1 to 7.

9. A use of the Portulaca oleracea extract according to claim 8, characterized in that: The purslane extract is used in a cosmetic emulsion, which comprises the following components by weight: 0.02-0.03 parts of sodium hyaluronate, 0.1-0.2 parts of xanthan gum, 3-5 parts of water-soluble lanolin, 0.8-1.0 parts of hydrogenated castor oil, 0.5-1.5 parts of glycerin, 0.5-0.8 parts of purslane extract, and 90-95 parts of deionized water.

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