Preparation process comprising composite strain fermentation process, product and application of product in preparation of composition for inhibiting skin tissue aging

Abstract

The invention belongs to the field of microbial fermentation technology and enzyme engineering, and particularly relates to a preparation process comprising a composite strain fermentation process, a product and application of the product in preparation of a composition for inhibiting skin tissue aging. According to the preparation process, macromolecular medium-temperature high-activity enzyme is obtained from a compound strain fermentation product through a molecular interception means, an enzymolysis technology is involved in application, the used enzyme is prepared by fermenting bacillus subtilis and bacillus amyloliquefaciens subsp. Plantarum compound strains, and by controlling enzymolysis conditions, after dendrobium nobile is subjected to enzymolysis, molecular interception is performed, so that the content of the dendrobium nobile in the dendrobium nobile is increased, and the content of the dendrobium nobile in the dendrobium nobile is increased. Enzyme and macromolecular impurities are fully removed, and the plant composition is prepared by compounding and drying with a saururus chinensis extracting solution or a saururus chinensis extract. The obtained plant composition can reduce the activity of MMPs protease, promote collagen synthesis, regulate the metabolism level of skin cells and excite the cell activity; and the skin care product has low cytotoxicity, can endow skin with glossiness and compactness permanently and effectively, and delays skin aging.

Description

Technical Field

[0001] The present invention belongs to the field of microbial fermentation technology and enzyme engineering, and specifically relates to a preparation process comprising a composite bacterial strain fermentation process, a product and an application thereof in preparing a composition for inhibiting skin tissue aging. Background Art

[0002] One of the main causes of skin aging is the change in the structure of the dermis. There are many reasons for the structural change. For example, external factors activate matrix metalloproteinases (MMPs) in the body, resulting in excessive degradation of collagen and elastin in the dermis that support the skin structure, causing wrinkles, decreased elasticity and other aging symptoms.

[0003] MMPs are a highly conserved class of proteases that can degrade virtually all components of the extracellular matrix. Typical MMPs consist of a propeptide of approximately 80 amino acids, a 170-amino acid metalloprotease catalytic domain, a variable-length linker peptide or hinge region, and a heme protein domain of approximately 200 amino acids. Based on their substrates and structure, MMPs can be divided into five major categories: collagenases, gelatinases, stromelysins, membrane-type MMPs, and other MMPs. MMPs primarily degrade collagen and elastin in the dermis, with MMP-1 primarily involved in the degradation of types I and III collagen. In young human skin, type I collagen and type III collagen account for 80% and 15% of the total collagen, respectively. With aging, type I and type III collagen are significantly lost, leading to a loss of elasticity and sagging. MMP-1 is the enzyme most responsible for the appearance of wrinkles, loss of elasticity, and other aging symptoms. Therefore, skin aging can be delayed by inhibiting MMP-1 activity and preventing the degradation of type I and III collagen.

[0004] At present, some products that delay skin aging achieve anti-aging effects by scavenging free radicals, while others achieve anti-aging effects by adding active ingredients that can stimulate collagen synthesis into skin care products.

[0005] For example, Chinese invention patent CN110833520A discloses an anti-aging composition, which includes the following components in parts by mass: 20-25 parts of coastal pine bark extract; 10-15 parts of bletilla striata root extract; 8-12 parts of polygonum multiflorum extract; and 17-25 parts of solvent. By using coastal pine bark extract, bletilla striata root extract and polygonum multiflorum extract to cooperate with each other, it is beneficial to better promote the human body's absorption of the effective ingredients in the anti-aging composition, better scavenge free radicals, and thus better delay the rate of skin aging.

[0006] Another Chinese invention patent publication number CN111671691A discloses a collagenase inhibitor composition, an anti-aging cream and a preparation method thereof. The collagenase inhibitor composition comprises 50-99% licorice root extract and 1-50% chamomile extract by mass, and the mass ratio of chamomile extract to licorice root extract is preferably 1:1-9; the anti-aging cream comprises the collagenase inhibitor composition and a penetration enhancer. Based on the total mass of the anti-aging cream, the amount of the collagenase inhibitor composition added is 0.01-10%, and the amount of the penetration enhancer added is 0.01-10%. The collagenase inhibitor composition comprises 50-99% licorice root extract and 1-50% chamomile extract, and the penetration enhancer is bis-diethoxydiglycol cyclohexane 1,4-dicarboxylate. The collagenase inhibitor composition uses licorice root extract and chamomile extract, which has a synergistic effect and can effectively inhibit the activity of collagenase; the anti-aging cream can reduce the degradation of collagen and play an anti-aging role. It also has moisturizing and water-locking properties, can prevent water loss, and create a moisturizing barrier for the skin.

[0007] However, the anti-aging compositions or creams provided by the aforementioned invention patents are ineffective in terms of anti-aging effects. Inhibiting the activity or expression of MMPs in the body can fundamentally improve free radical scavenging efficiency, addressing the problem of collagen loss, and thus achieving a lasting and effective effect in delaying skin aging. Therefore, there is an urgent need in the art to develop a composite bacterial fermentation process that can effectively inhibit the activity or expression of MMPs, and to apply the resulting product to anti-aging products. Summary of the Invention

[0008] In view of the problems existing in the prior art, the present invention provides a preparation process comprising a composite bacterial strain fermentation process, a product and an application thereof in preparing a composition for inhibiting skin tissue aging.

[0009] To achieve the above object, the technical solution adopted by the present invention is as follows: In one aspect, the present invention provides a preparation process comprising a composite bacterial strain fermentation process, the preparation process comprising: 75-90%wt of Saururus chinensis extract is mixed with 10-25%wt of Dendrobium officinale extract obtained by enzymatic hydrolysis; or 75-90%wt of Saururus chinensis extract is mixed with 10-25%wt of Dendrobium officinale extract obtained by enzymatic hydrolysis; the Saururus chinensis extract is obtained by drying the Saururus chinensis extract, and the Dendrobium officinale extract is obtained by drying the Dendrobium officinale extract.

[0010] Specifically, the preparation process comprises: adding 75-76% wt, 76-77% wt, 77-78% wt, 78-79% wt, 79-80% wt, 80-81% wt, 81-82% wt, 82-83% wt, 83-84% wt, 84-85% wt, 85-86% wt, 86-87% wt, 87-88% wt, 88-89% wt or 89-90% wt of the three Dendrobium extract obtained by enzymatic hydrolysis of Herba Lycopodii extract and 10-11%wt, 11-12%wt, 12-13%wt, 13-14%wt, 14-15%wt, 15-16%wt, 16-17%wt, 17-18%wt, 18-19%wt, 19-20%wt, 20-21%wt, 21-22%wt, 22-23%wt, 23-24%wt or 24-25%wt Mixing; or 75-76% wt, 76-77% wt, 77-78% wt, 78-79% wt, 79-80% wt, 80-81% wt, 81-82% wt, 82-83% wt, 83-84% wt, 84-85% wt, 85-86% wt, 86-87% wt, 87-88% wt, 88-89% wt or 89-90% wt of Saururus chinensis extract with 10-1 10-25%wt of a dendrobium extract obtained by enzymatic hydrolysis of 1%wt, 11-12%wt, 12-13%wt, 13-14%wt, 14-15%wt, 15-16%wt, 16-17%wt, 17-18%wt, 18-19%wt, 19-20%wt, 20-21%wt, 21-22%wt, 22-23%wt, 23-24%wt or 24-25%wt is mixed; Preferably, the preparation process comprises: mixing 75-85%wt of Saururus chinensis extract with 10-25%wt of Dendrobium officinale extract obtained by enzymatic hydrolysis; or mixing 75-85%wt of Saururus chinensis extract with 15-25%wt of Dendrobium officinale extract obtained by enzymatic hydrolysis.

[0011] The preparation method of the Saururus chinensis extract comprises the following steps: S1. Preliminary extraction: Extract Saururus chinensis by adding 10-20 times its weight of water, filter, and collect the aqueous extract; then repeat the extraction 0-2 times with the medicinal residue, and combine to obtain a combined aqueous extract; S2, concentration and filtration: The combined aqueous extracts were concentrated to 4-6 times the weight of Saururus chinensis, and filtered to obtain filtrate 1; S3, concentration and sedimentation; filtrate 1 is concentrated to 0.5-2 times the weight of Saururus chinensis, ethanol is added to a final concentration of ethanol of 60-70% v / v, sedimentation is carried out, and the supernatant is collected; S4, decolorization: add 0.5-2%wt activated carbon to the supernatant to decolorize, filter, and obtain filtrate 2; S5, concentration: Filtrate 2 is concentrated to 0.3-0.5 times the weight of Saururus chinensis to obtain Saururus chinensis extract; The method for preparing the dendrobium extract obtained by enzymatic hydrolysis comprises the following steps: (1) Preliminary extraction: Add 50-100 times the weight of water to the dendrobium, filter, and obtain the dendrobium primary extract; (2) Concentration under reduced pressure: The primary extract of Dendrobium officinale is concentrated to 10-20 times the weight of Dendrobium officinale to obtain a concentrated solution; (3) Enzymatic hydrolysis: add 0.01-0.02% w / v medium-temperature amylase to the concentrate and perform enzymatic hydrolysis at 5-10°C for 20-40 min to obtain an enzymatic hydrolyzate; (4) Enzyme removal and impurity removal: The enzymatic hydrolysate is filtered through a 6-20 kD filter membrane to remove macromolecules and enzymes to obtain an ultrafiltrate; (5) Decolorization: Add 0.5-2%wt activated carbon to the ultrafiltrate for decolorization, and filter to obtain a filtrate; (6) Membrane filtration and concentration: The filtrate is filtered through a membrane and concentrated to 0.3-0.5 times the weight of the dendrobium to obtain the dendrobium extract; The mesophilic amylase is obtained through a composite bacterial fermentation process. The preparation method comprises: mixing Bacillus subtilis CMCC(B) 63501 and Bacillus amyloliquefaciens plantarum subspecies BNCC190056 in a live bacterial count ratio of 1:1, inoculating the mixture into a culture medium at a 5% v / v inoculation rate, centrifuging the mixture after fermentation, collecting the supernatant, removing small molecular impurities, collecting the concentrated solution, and purifying the mixture to obtain the mesophilic amylase.

[0012] Preferably, the extraction conditions in step S1 or step (1) are 90-100° C. for 0.5-1.5 h.

[0013] Preferably, the sedimentation condition in step S3 is 20-25° C. for 2-3 hours.

[0014] Preferably, the decolorization conditions in step S4 or step (5) are 20-30° C. for 30-60 min.

[0015] Preferably, the filter membrane described in step (6) is a 300-400D filter membrane.

[0016] Preferably, the fermentation condition is 37±2°C for 24-48 hours.

[0017] Preferably, the removal of small molecule impurities comprises: using 20kD tangential membrane filtration and concentration to remove small molecule impurities.

[0018] In another aspect, the present invention provides a plant composition prepared by any of the above processes.

[0019] In another aspect, the present invention provides use of any of the above-mentioned plant compositions in the preparation of anti-aging products.

[0020] Specifically, the products include cosmetics or medicines.

[0021] Specifically, the product contains at least 0.2%wt of the plant composition.

[0022] Preferably, the product contains 0.2-1%wt of the plant composition.

[0023] In another aspect, the present invention provides a cosmetic comprising any one of the above-mentioned plant compositions.

[0024] Preferably, the cosmetics include any one or more of face cream, facial mask, eye cream, neck cream, hand cream, body lotion, moisturizing lotion, essence, toner, shampoo, conditioner, shower gel or cleanser.

[0025] Specifically, the cosmetics also include auxiliary materials acceptable in the cosmetics field.

[0026] Preferably, the cosmetics acceptable excipients include: one or more of moisturizers, thickeners, emollients, skin conditioners, emulsifiers, preservatives, emollients, lubricants, and stabilizers.

[0027] In another aspect, the present invention provides a medicine comprising any one of the above-mentioned plant compositions.

[0028] Specifically, the medicine also includes pharmaceutically acceptable excipients.

[0029] Preferably, the pharmaceutically acceptable excipients include, but are not limited to, solvents, diluents, disintegrants, precipitation inhibitors, surfactants, glidants, adhesives, lubricants, dispersants, suspending agents, isotonic agents, thickeners, emulsifiers, preservatives, stabilizers, hydrating agents, emulsification accelerators, buffers, absorbents, colorants, flavoring agents, sweeteners, ion exchangers, release agents, coating agents, flavoring agents, or antioxidants.

[0030] Specifically, the dosage form of the drug includes, but is not limited to, a gastrointestinal dosage form and a parenteral dosage form according to the administration method.

[0031] Preferably, the dosage forms for administration via the gastrointestinal tract include but are not limited to tablets, powders, granules, solutions, capsules, emulsions, suspensions, and oils.

[0032] Preferably, the gastrointestinal administration dosage form includes but is not limited to an injection dosage form, a respiratory tract administration dosage form, a skin administration dosage form, a mucosal administration dosage form and a cavity administration dosage form.

[0033] In another aspect, the present invention provides an anti-aging method, comprising using any one of the above-mentioned plant compositions, cosmetics or medicines.

[0034] Compared with the prior art, the present invention has the following beneficial effects: (1) The present invention screens and optimizes the preparation process of Saururus chinensis extract and Dendrobium officinale extract. In the preparation process, the composite bacterial fermentation product is obtained by molecular interception to obtain a macromolecular medium-temperature high-activity enzyme. The application involves enzymatic hydrolysis technology. The enzyme used is prepared by fermentation of a composite bacterial strain of Bacillus subtilis and Bacillus amyloliquefaciens subspecies. By controlling the enzymatic hydrolysis conditions, the Dendrobium officinale is enzymatically hydrolyzed, and the molecules are intercepted to fully remove the enzyme and macromolecular impurities. The plant composition is prepared by drying the composite Saururus chinensis extract or the Saururus chinensis extract. The preparation process of the present invention increases the content of the active ingredient, can better play the function of inhibiting the activity of MMPs, solves the problem of gradual loss of collagen and loss of elasticity of elastic fibers, and maintains the skin in an optimal state of fineness and elasticity.

[0035] (2) The present invention obtains a plant composition that synergistically inhibits the expression of MMPs through the product of the composite bacterial fermentation process and the enzymatic hydrolysis technology. The synergistic effect between the extract of Saururus chinensis and the extract of Dendrobium officinale can inhibit the expression of MMP-1 and MMP-3, reduce the activity of MMPs-like proteases, promote collagen synthesis, regulate the metabolism level of skin cells, and stimulate cell vitality. It has low cytotoxicity and can give the skin long-lasting and effective gloss and firmness, thereby delaying skin aging. DETAILED DESCRIPTION

[0036] The application will be further clarified by the following examples. The examples are only a part of the application and are not used to limit the application. The experimental methods used in the following examples are conventional experiments. The materials and reagents used in the following examples are commercially available unless otherwise specified. Bacillus subtilis CMCC(B) 63501 was purchased from Shanghai Lumi Technology Co., Ltd. Bacillus amyloliquefaciens plant subspecies BNCC190056 was purchased from Beijing Beina Link Biological Technology. Thermostable amylase was purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd. with the product code FDY-3002. Cellulase was purchased from Beijing Weinuo Biological Technology Co., Ltd. with the batch number 2024010503. β-glucanase was purchased from Beijing Weinuo Biological Technology Co., Ltd. with the batch number 2024021105. β-mannanase was purchased from Beijing Weinuo Biological Technology Co., Ltd. with the batch number 2024011801. Human fibroblast cells (NHDF cells) were purchased from the Chinese Academy of Sciences Cell Bank. DMEM medium was purchased from Gibco. t-BHP was purchased from GlpBio. MolPure® Cell RNA Kit was purchased from Yixing Biological Technology. Evo-MLV reverse transcription premix kit was purchased from Aikangrui Biological. SYBR ® Green Pro Taq HS premix qPCR kit was purchased from Aikangrui Biological.

[0037] Example 1 Preparation process 1. Preparation of mesophilic amylase Strain activation: Bacillus subtilis CMCC(B) 63501 and Bacillus amyloliquefaciens plant subspecies BNCC190056 were inoculated into LB medium and cultured at 37°C for 24 hours to activate the strains Bacillus subtilis CMCC(B) 63501 and Bacillus amyloliquefaciens plant subspecies BNCC190056.

[0038] Bacillus subtilis CMCC(B) 63501 and Bacillus amyloliquefaciens plant subspecies BNCC190056 were mixed at a ratio of 1:1 of viable bacterial amount, inoculated into LB medium at a ratio of 5% v / v, and fermented at 37°C for 24 hours. After fermentation, the fermentation broth was centrifuged at 8000 rpm for 10 min to remove the bacterial bodies. The concentrated solution was collected after removing small molecular impurities by using 20kD tangential membrane filtration and purification.

[0039] 2. Preparation of threeleaf milkwort extract S1, preliminary extraction: 100g of threeleaf milkwort was added to 1000g of water, and extracted at 95°C for 1h to remove the residue and obtain the water extract; S2, concentration and filtration: The aqueous extract is concentrated to 400 g, filtered until clear, and filtrate 1 is obtained; S3, concentration and sedimentation: Filtrate 1 was concentrated under reduced pressure to 50 g, ethanol was added to make the final concentration of ethanol 60% v / v, the temperature was controlled at 20°C, sedimentation was carried out for 2 h, and the supernatant was collected; S4, decolorization: take 120 g of the supernatant, add 0.6 g of activated carbon, stir and decolorize at 20°C for 30 min, filter until clear, and obtain filtrate 2; S5. Concentration: Filtrate 2 is concentrated to 30 g to obtain Saururus chinensis extract.

[0040] 3. Preparation of Dendrobium Extract S1. Preliminary extraction: 100 g of coarsely crushed Dendrobium officinale was added with 5000 g of water, extracted at 95°C for 1 h, and filtered until clear to obtain the primary extract of Dendrobium officinale; S2, vacuum concentration: the primary extract of Dendrobium is concentrated under reduced pressure to 1000 g to obtain a concentrated solution; S3, enzymatic hydrolysis: add 0.2g of mesophilic amylase to 1000mL of the concentrate, maintain at 5℃, and enzymatically hydrolyze for 20min to obtain an enzymatic hydrolyzate; S4, enzyme removal and impurity removal: The enzymatic hydrolyzate was filtered through a 6kD filter membrane to remove macromolecular impurities and enzymes to obtain 860g of ultrafiltrate; S5. Decolorization: Add 4.3 g of activated carbon to the ultrafiltrate, stir and decolorize at 20°C for 30 min, filter until clear, and obtain a filtrate; S6. Membrane filtration and concentration: The filtrate is filtered through a 300D filter membrane to remove small molecular impurities and water, and concentrated to 30 g to obtain the dendrobium extract.

[0041] 3. Preparation of plant compositions 30 g of Saururus chinensis extract and 10 g of Dendrobium officinale extract were mixed evenly and dried to obtain 24 g of the plant composition.

[0042] Example 2 Preparation process 1. Preparation of medium-temperature amylase The preparation method is the same as that in Example 1 to obtain medium-temperature amylase.

[0043] 2. Preparation of Saururus chinensis Extract S1. Preliminary extraction: 100 g of Saururus chinensis was added to 1500 g of water, extracted at 95°C for 1 h, and the residue was removed to obtain aqueous extract 1; the residue was added to 1500 g of water, extracted at 95°C for 1 h, and the residue was removed to obtain aqueous extract 2; S2, concentration and filtration: combining aqueous extract 1 and aqueous extract 2 to obtain a combined aqueous extract, concentrating the combined aqueous extract to 500 g, and filtering until clear to obtain filtrate 1; S3, concentration and sedimentation: Filtrate 1 was concentrated under reduced pressure to 100 g, ethanol was added to make the final concentration of ethanol 65% v / v, the temperature was controlled at 25°C, sedimentation was carried out for 2 h, and the supernatant was collected; S4, decolorization: take 280 g of the supernatant, add 2.8 g of activated carbon, stir and decolorize at 30°C for 45 min, filter until clear, and obtain filtrate 2; S5. Concentration: Filtrate 2 is concentrated to 40 g to obtain Saururus chinensis extract.

[0044] 3. Preparation of Dendrobium Extract S1. Preliminary extraction: 100 g of coarsely crushed Dendrobium officinale was dried and added with 7500 g of water. The mixture was extracted at 95°C for 1 h and filtered until clear to obtain the primary extract of Dendrobium officinale. S2, vacuum concentration: the primary extract of Dendrobium is concentrated under reduced pressure to 1500 g to obtain a concentrated solution; S3, enzymatic hydrolysis: add 0.2g of medium-temperature amylase to 1500mL of the concentrate, maintain at 5℃, and enzymatically hydrolyze for 30min to obtain an enzymatic hydrolyzate; S4, enzyme removal and impurity removal: The enzymatic hydrolyzate was filtered through a 10 kD filter membrane to remove macromolecular impurities and enzymes to obtain 1350 g of ultrafiltrate; S5. Decolorization: Add 13.5 g of activated carbon to the ultrafiltrate, stir and decolorize at 30°C for 50 min, and filter until clear to obtain a filtrate; S6. Membrane filtration and concentration: The filtrate is filtered through a 300D filter membrane to remove small molecular impurities and water, and concentrated to 40 g to obtain the dendrobium extract.

[0045] 4. Preparation of plant compositions 30 g of Saururus chinensis extract and 7.5 g of Dendrobium officinale extract were mixed evenly and dried to obtain 22 g of the plant composition.

[0046] Example 3 Preparation process 1. Preparation of medium-temperature amylase The preparation method is the same as that in Example 1 to obtain medium-temperature amylase.

[0047] 2. Preparation of Saururus chinensis Extract S1. Preliminary extraction: 100 g of Saururus chinensis was added to 2000 g of water, extracted at 95°C for 1 h, and the residue was removed to obtain aqueous extract 1; the residue was added to 2000 g of water, extracted at 95°C for 1 h, and the residue was removed to obtain aqueous extract 2; the residue was further added to 2000 g of water, extracted at 95°C for 1 h, and the residue was removed to obtain aqueous extract 3; S2, concentration and filtration: combining aqueous extract 1, aqueous extract 2 and aqueous extract 3 to obtain a combined aqueous extract, concentrating the combined aqueous extract to 600 g, and filtering until clear to obtain filtrate 1; S3, concentration and sedimentation: Filtrate 1 was concentrated under reduced pressure to 200 g, ethanol was added to make the final concentration of ethanol 70% v / v, the temperature was controlled at 20°C, sedimentation was carried out for 3 h, and the supernatant was collected; S4, decolorization: take 650 g of the supernatant, add 13 g of activated carbon, stir and decolorize at 25°C for 60 min, filter until clear, and obtain filtrate 2; S5. Concentration: Filtrate 2 is concentrated to 50 g to obtain Saururus chinensis extract.

[0048] 3. Preparation of Dendrobium Extract S1. Preliminary extraction: 100 g of coarsely crushed Dendrobium officinale was added with 10,000 g of water, extracted at 95°C for 1 h, and filtered until clear to obtain the primary extract of Dendrobium officinale; S2, vacuum concentration: the primary extract of Dendrobium is concentrated under reduced pressure to 2000 g to obtain a concentrated solution; S3, enzymatic hydrolysis: add 0.2g of medium-temperature amylase to 2000mL of the concentrate, maintain at 10℃, and enzymatically hydrolyze for 40min to obtain an enzymatic hydrolyzate; S4, enzyme removal and impurity removal: The enzymatic hydrolyzate was filtered through a 20 kD filter membrane to remove macromolecular impurities and enzymes to obtain 1815 g of ultrafiltrate; S5. Decolorization: Add 36.3 g of activated carbon to the ultrafiltrate, stir and decolorize at 25°C for 60 min, filter until clear, and obtain a filtrate; S6. Membrane filtration and concentration: The filtrate is filtered through a 300D filter membrane to remove small molecular impurities and water, and concentrated to 50 g to obtain the dendrobium extract.

[0049] 4. Preparation of plant compositions 27 g of Saururus chinensis extract and 3 g of Dendrobium officinale extract were mixed evenly and dried to obtain 19 g of the plant composition.

[0050] Example 4 Preparation process The preparation process of Example 4 differs from that of Example 2 only in "4. Preparation of plant composition". The specific process is as follows: The extract of Saururus chinensis or Dendrobium officinale was dried to obtain Saururus chinensis extract and Dendrobium officinale extract, respectively. 18g of Saururus chinensis extract and 6g of Dendrobium officinale extract were mixed evenly to obtain 24g of a plant composition.

[0051] Comparative Example 1 Preparation process of Saururus chinensis extract 1. Preparation of Saururus chinensis Extract The preparation method is the same as that in Example 2 to obtain the Saururus chinensis extract.

[0052] 2. Preparation of Saururus chinensis Extract After the Saururus chinensis extract was dried, 21 g of Saururus chinensis extract was obtained.

[0053] Comparative Example 2 Preparation process of Dendrobium extract 1. Preparation of Dendrobium extract The preparation method is the same as that in Example 2 to obtain the dendrobium extract.

[0054] 2. Preparation of Dendrobium Extract The dendrobium extract was dried to obtain 16 g of dendrobium extract.

[0055] Comparative Example 3 Preparation Process 1. Preparation of medium-temperature amylase The preparation method is the same as that in Example 1 to obtain medium-temperature amylase.

[0056] 2. Preparation of Saururus chinensis Extract S1, preliminary extraction: 100g of Saururus chinensis was added to 3000g of water, extracted at 95℃ for 1h, and the residue was removed for standby use to obtain aqueous extract 1; the residue was added to 3000g of water, extracted at 95℃ for 1h, and the residue was removed for standby use to obtain aqueous extract 2; the residue was added to 3000g of water, extracted at 95℃ for 1h, and the residue was removed for standby use to obtain aqueous extract 3; the residue was added to 3000g of water, extracted at 95℃ for 1h, and the residue was removed for standby use to obtain aqueous extract 4; S2, concentration and filtration: combining aqueous extract 1, aqueous extract 2, aqueous extract 3 and aqueous extract 4 to obtain a combined aqueous extract, concentrating to 300 g, and filtering until clear to obtain filtrate 1; S3, concentration and sedimentation: Filtrate 1 was concentrated under reduced pressure to 250 g, ethanol was added to make the final concentration of ethanol 50% v / v, the temperature was controlled at 25°C, sedimentation was carried out for 2 h, and the supernatant was collected; S4, decolorization: take 480 g of the supernatant, add 4.8 g of activated carbon, stir and decolorize at 30°C for 45 min, filter until clear, and obtain filtrate 2; S5. Concentration: Filtrate 2 is concentrated to 60 g to obtain Saururus chinensis extract.

[0057] 3. Preparation of Dendrobium Extract S1. Preliminary extraction: 100 g of coarsely crushed Dendrobium officinale was dried and added with 2500 g of water. The mixture was extracted at 95°C for 1 h and filtered until clear to obtain the primary extract of Dendrobium officinale. S2, vacuum concentration: the primary extract of Dendrobium is concentrated under reduced pressure to 800 g to obtain a concentrated solution; S3, enzymatic hydrolysis: add 0.2g of medium-temperature amylase to 800mL of the concentrate, maintain at 5℃, and enzymatically hydrolyze for 30min to obtain an enzymatic hydrolyzate; S4, enzyme removal and impurity removal: The enzymatic hydrolyzate was filtered through a 10 kD filter membrane to remove macromolecular impurities and enzymes to obtain 1350 g of ultrafiltrate; S5. Decolorization: Add 13.5 g of activated carbon to the ultrafiltrate, stir and decolorize at 30°C for 50 min, and filter until clear to obtain a filtrate; S6. Membrane filtration and concentration: The filtrate is filtered through a 300D filter membrane to remove small molecular impurities and water, and concentrated to 40 g to obtain the dendrobium extract.

[0058] 4. Preparation of plant compositions 30 g of Saururus chinensis extract and 7.5 g of Dendrobium officinale extract were mixed evenly and dried to obtain 20 g of the plant composition.

[0059] Comparative Example 4 Preparation Process 1. Preparation of medium-temperature amylase The preparation method is the same as that in Example 1 to obtain medium-temperature amylase.

[0060] 2. Preparation of Saururus chinensis Extract The preparation method is the same as that in Example 1 to obtain the Saururus chinensis extract.

[0061] 3. Preparation of Dendrobium Extract S1. Preliminary extraction: 100 g of coarsely crushed Dendrobium officinale was dried and added with 7500 g of water. The mixture was extracted at 95°C for 1 h and filtered until clear to obtain the primary extract of Dendrobium officinale. S2, vacuum concentration: the primary extract of Dendrobium is concentrated under reduced pressure to 1500 g to obtain a concentrated solution; S3, enzymatic hydrolysis: add 0.2g of medium-temperature amylase to 1500mL of the concentrate, maintain at 15℃, and enzymatically hydrolyze for 60min to obtain an enzymatic hydrolyzate; S4, enzyme removal and impurity removal: the enzymatic hydrolyzate is filtered through a 5kD filter membrane to remove macromolecular impurities and enzymes to obtain 1200g of ultrafiltrate; S5. Decolorization: Add 12 g of activated carbon to the ultrafiltrate, stir and decolorize at 30°C for 50 min, and filter until clear to obtain a filtrate; S6. Membrane filtration and concentration: The filtrate is filtered through a 300D filter membrane to remove small molecular impurities and water, and concentrated to 40 g to obtain the dendrobium extract.

[0062] 4. Preparation of plant compositions 30 g of Saururus chinensis extract and 7.5 g of Dendrobium officinale extract were mixed evenly and dried to obtain 22 g of the plant composition.

[0063] Comparative Example 5 Preparation Process The preparation process of Comparative Example 5 differs from that of Example 2 only in that: "1. Preparation of medium-temperature amylase" is different, and the specific process is as follows: Strain activation: Bacillus subtilis CMCC(B) 63501 was inoculated into LB medium and cultured at 37°C for 24 hours to activate the strain Bacillus subtilis CMCC(B) 63501.

[0064] Bacillus subtilis CMCC(B) 63501 was inoculated into LB medium at a 5% v / v inoculum and fermented at 37°C for 24 hours. After the fermentation, the fermentation broth was centrifuged at 8000 rpm for 10 minutes to remove the bacteria, and concentrated using a 20 kD tangential membrane to remove small molecular impurities. The concentrate was collected and purified to obtain the mesophilic amylase.

[0065] Comparative Example 6 Preparation Process The preparation process of Comparative Example 6 differs from that of Example 2 only in that: "1. Preparation of medium-temperature amylase" is different, and the specific process is as follows: Strain activation: Bacillus amyloliquefaciens plantarum subsp. BNCC190056 was inoculated into LB medium and cultured at 37° C. for 24 hours to activate the strain Bacillus amyloliquefaciens plantarum subsp. BNCC190056.

[0066] Bacillus amyloliquefaciens plantarum subspecies BNCC190056 was inoculated into LB medium at a rate of 5% v / v and fermented at 37°C for 24 hours. After the fermentation, the fermentation broth was centrifuged at 8000 rpm for 10 minutes to remove the bacteria, and concentrated using a 20 kD tangential membrane to remove small molecular impurities. The concentrate was collected and purified to obtain the mesophilic amylase.

[0067] Comparative Example 7 Preparation Process The preparation process of Comparative Example 7 differs from that of Example 2 only in that "Step S3, enzymatic hydrolysis" in "3. Preparation of Dendrobium Extract" is different. The specific process is as follows: S3. Enzymolysis: Add 0.2 g of high-temperature amylase to 1500 mL of the concentrated solution, maintain at 5°C, and perform enzymolysis for 30 min to obtain an enzymolysis solution.

[0068] Comparative Example 8 Preparation Process The preparation process of Comparative Example 8 differs from that of Example 2 only in that "Step S3, enzymatic hydrolysis" in "3. Preparation of Dendrobium Extract" is different. The specific process is as follows: S3. Enzyme hydrolysis: add 0.2 g of cellulase to 1500 mL of the concentrated solution, maintain at 5°C, and perform enzymolysis for 30 min to obtain an enzymatic hydrolyzate.

[0069] Comparative Example 9 Preparation Process The preparation process of Comparative Example 9 differs from that of Example 2 only in that "Step S3, enzymatic hydrolysis" in "3. Preparation of Dendrobium Extract" is different. The specific process is as follows: S3. Enzymatic hydrolysis: add 0.2 g of β-glucanase to 1500 mL of the concentrated solution, maintain the temperature at 5°C, and perform enzymatic hydrolysis for 30 min to obtain an enzymatic hydrolyzate.

[0070] Comparative Example 10 Preparation Process The preparation process of Comparative Example 10 differs from that of Example 2 only in that "Step S3, enzymatic hydrolysis" in "3. Preparation of Dendrobium Extract" is different. The specific process is as follows: S3. Enzymatic hydrolysis: add 0.2 g of β-mannanase to 1500 mL of the concentrated solution, maintain the temperature at 5°C, and perform enzymatic hydrolysis for 30 min to obtain an enzymatic hydrolyzate.

[0071] Comparative Example 11 Preparation Process 1. Preparation of medium-temperature amylase The preparation method is the same as that in Example 1 to obtain medium-temperature amylase.

[0072] 2. Preparation of Saururus chinensis Extract S1. Preliminary extraction: 100 g of Saururus chinensis was added to 1500 g of water, extracted at 95°C for 1 h, and the residue was removed to obtain aqueous extract 1; the residue was added to 1500 g of water, extracted at 95°C for 1 h, and the residue was removed to obtain aqueous extract 2; S2, concentration and filtration: combining aqueous extract 1 and aqueous extract 2 to obtain a combined aqueous extract, and concentrating the combined aqueous extract to 500 g to obtain a concentrated solution; S3, decolorization: add 5g activated carbon to the concentrate, stir and decolorize at 30℃ for 45min, filter until clear, and obtain filtrate 1; S4, concentration and sedimentation: Filtrate 1 was concentrated under reduced pressure to 100 g, ethanol was added to make the final concentration of ethanol 65% v / v, the temperature was controlled at 25°C, sedimentation was carried out for 2 h, and the supernatant was collected; S5. Decolorization: Take 280 g of the supernatant, add 2.8 g of activated carbon, stir and decolorize at 30°C for 45 min, filter until clear, and obtain filtrate 2; S6. Concentration: Filtrate 2 is concentrated to 40 g to obtain Saururus chinensis extract.

[0073] 3. Preparation of Dendrobium Extract S1. Preliminary extraction: 100 g of coarsely crushed Dendrobium officinale was dried and added with 7500 g of water. The mixture was extracted at 95°C for 1 h and filtered until clear to obtain the primary extract of Dendrobium officinale. S2, decolorization: The primary extract of Dendrobium officinale was concentrated under reduced pressure to 1500 g, 15 g of activated carbon was added, and the mixture was stirred at 30°C for 50 min for decolorization, and filtered until clear to obtain filtrate 1; S3, enzymatic hydrolysis: add 0.2g of mesophilic amylase to 1500mL of filtrate 1, maintain at 5℃, and enzymatically hydrolyze for 30min to obtain enzymatic hydrolyzate; S4, enzyme removal and impurity removal: The enzymatic hydrolyzate was filtered through a 10 kD filter membrane to remove macromolecular impurities and enzymes to obtain 1350 g of ultrafiltrate; S5. Decolorization: Add 13.5 g of activated carbon to the ultrafiltrate, stir and decolorize at 30°C for 50 min, and filter until clear to obtain filtrate 2; S6, Membrane filtration and concentration: The filtrate 2 was filtered through a 300D filter membrane to remove small molecular impurities and water, and concentrated to 40 g to obtain the dendrobium extract.

[0074] 4. Preparation of plant composition After 30 g of the threeleaf milkwort extract and 7.5 g of the dendrobium extract were uniformly mixed, the plant composition was dried to obtain 22 g of the plant composition.

[0075] Preparation process of Comparative Example 12 1. Preparation of mesophilic amylase The mesophilic amylase was prepared by the same method as in Example 1.

[0076] 2. Preparation of threeleaf milkwort extract S1, Preliminary extraction: 100 g of threeleaf milkwort was added to 1500 g of water, and extracted at 95°C for 1 h to remove the residue and obtain water extract 1; the residue was added to 1500 g of water, and extracted at 95°C for 1 h to remove the residue and obtain water extract 2; S2, Concentration and filtration: the water extract 1 and the water extract 2 were combined to obtain combined water extract, which was concentrated to 500 g and filtered to be clear to obtain filtrate 1; S3, Concentration and sedimentation: the filtrate 1 was concentrated under reduced pressure to 100 g, and ethanol was added to a final concentration of 65% v / v, and the temperature was controlled at 25°C, and the supernatant was collected after 2 h of sedimentation; S4, Decolorization: 280 g of the supernatant was added to 0.84 g of activated carbon, and stirred at 30°C for 20 min to decolorize, and filtered to be clear to obtain filtrate 2; S5, Concentration: the filtrate 2 was concentrated to 40 g to obtain the threeleaf milkwort extract.

[0077] 3. Preparation of dendrobium extract S1, Preliminary extraction: 100 g of dried and roughly crushed dendrobium was added to 7500 g of water, and extracted at 95°C for 1 h to filter to be clear to obtain dendrobium preliminary extract; S2, Concentration under reduced pressure: the dendrobium preliminary extract was concentrated under reduced pressure to 1500 g to obtain concentrated solution; S3, Enzymolysis: 0.2 g of mesophilic amylase was added to 1500 mL of the concentrated solution, and the temperature was maintained at 5°C for 30 min to obtain enzyme solution; S4, Enzyme removal and impurity removal: the enzyme solution was filtered through a 10 kD filter membrane to remove large molecular impurities and enzymes to obtain 1350 g of ultrafiltrate; S5, Decolorization: 4.05 g of activated carbon was added to the ultrafiltrate, and stirred at 30°C for 20 min to decolorize, and filtered to be clear to obtain filtrate; S6, Membrane filtration and concentration: the filtrate was filtered through a 300D filter membrane to remove small molecular impurities and water, and concentrated to 40 g to obtain the dendrobium extract.

[0078] 4. Preparation of plant composition 30 g of Saururus chinensis extract and 7.5 g of Dendrobium officinale extract were mixed evenly and dried to obtain 22 g of the plant composition.

[0079] Comparative Example 13 Preparation Process The preparation process of Comparative Example 13 differs from that of Example 2 only in "4. Preparation of plant composition". The specific process is as follows: 30 g of Saururus chinensis extract and 30 g of Dendrobium officinale extract were mixed evenly and dried to obtain 48 g of the plant composition.

[0080] Experimental Example 1 Cytotoxicity Assay 1. Experimental methods The samples to be tested are the plant compositions obtained by the preparation processes of Examples 1 to 4, the Saururus chinensis extract of Comparative Example 1, the Dendrobium candidum extract of Comparative Example 2, and the plant compositions obtained by the preparation processes of Comparative Examples 3 to 13, which are dissolved in deionized water under sterile conditions, and the final concentration is 100% (equivalent to the mass fraction of the crude drug amount).

[0081] Under sterile conditions, the samples to be tested were diluted with DMEM medium to a mass concentration of 0.04%, 0.2%, 1% and 2.5% respectively; human fibroblasts were cultured at a density of 5×10 3 The cells were seeded at a density of 100 μL / well in a 96-well plate and cultured in a 37°C, 5% CO2 cell culture incubator for 24 hours. When the cell density reached about 50%, 100 μL of the sample at different concentrations was added for treatment. The DMEM medium was used as a control (the measurement results were recorded as blank control OD 450nm ), with the wells without cells as the background (the measurement results were recorded as background OD 450nm ). 24 h after sample addition, cell activity was detected using the CCK-8 method, the absorbance was measured at 450 nm using a microplate reader, and the cell survival rate was calculated.

[0082] Cell viability (%) = (OD 450nm - Background OD 450nm )÷(blank control group OD 450nm - Background OD 450nm )×100%.

[0083] 2. Experimental results The cell viability results are shown in Table 1. When the mass concentration of the test sample is 0.04-2.5%, the cell viability of Examples 1 to 4 reaches more than 92%, indicating that the plant composition obtained by the preparation process of the present invention has low cytotoxicity. The test sample is selected at a mass concentration of 0.04-1% for subsequent experiments.

[0084] Table 1 CCK-8 test results

[0085] Note: NC in the table stands for blank control.

[0086] Experimental Example 2 Anti-aging efficacy test 1. Experimental methods The samples to be tested are the plant compositions obtained by the preparation processes of Examples 1 to 4, the Saururus chinensis extract of Comparative Example 1, the Dendrobium officinale extract of Comparative Example 2, and the plant compositions obtained by the preparation processes of Comparative Examples 3 to 13.

[0087] According to the results of cytotoxicity test, three concentrations (0.04%, 0.2%, and 1%) were selected as the anti-aging efficacy test concentrations. Human fibroblasts were cultured at 2×10 5 Cells were seeded at a density of 100 μL / well in a 6-well plate and cultured in a 37°C, 5% CO2 incubator for 24 hours. The cells were then divided into a blank control (NC), a tBHP group, and a test sample group. 200 μM tBHP was added to each of the tBHP and sample groups, followed by stimulation for 2 hours and then washed away. Only the test sample group was then added with 0.1 mL of the test sample at the corresponding concentration. The cells were then cultured in a 37°C, 5% CO2 incubator for 6 hours. The blank control group received no treatment. The treatments for each group are shown in Table 2.

[0088] Table 2 Treatment methods of each group

[0089] At the end of the time period, total RNA from all cells in each well was collected using the MolPure® Cell RNA Kit according to the instructions. The collected total RNA was then reverse-transcribed into cDNA using the Evo-MLV Reverse Transcription Premix Kit according to the instructions. Finally, qPCR fluorescence quantitative analysis was performed using the SYBR® Green Pro Taq HS Premix qPCR Kit to detect the expression levels of MMP-1 and MMP-3, and the inhibition rates of MMP-1 and MMP-3 expression were calculated.

[0090] Inhibition rate %=((tBHP group-blank control group)-(sample group-blank control group)) / (tBHP group-blank control group)×100%.

[0091] The gene names of the PCR primers are β-actin, MMP-1, and MMP-3. The PCR primers were synthesized by Shanghai Sangon Biotechnology Co., Ltd., and the sequences are shown in Table 3: Table 3 Primer sequences

[0092] Note: F in the table represents forward primer; R represents reverse primer.

[0093] The Combination Index (CI) is a method for evaluating drug combinations, proposed by Chou and Talalay in 1984. This method, based on the dose-response curve, compares the actual effect achieved with the theoretical effect of the combination to determine whether the combination exhibits synergistic, additive, or antagonistic effects. CompuSyn software is used to analyze the calculated inhibition rate to generate the CI value. A CI value less than 0.9 indicates a synergistic effect; smaller values ​​indicate a stronger synergistic effect; a CI value between 0.9 and 1.1 indicates an additive effect; and a CI value greater than 1.1 indicates an antagonistic effect; larger values ​​indicate a stronger antagonistic effect.

[0094] 2. Experimental results The results of the relative expression level, expression inhibition rate and CI value of MMP-1 are shown in Table 4. As shown in Table 4, the plant composition obtained by the preparation process of Examples 1 to 4 can significantly inhibit the relative expression level of MMP-1 in human fibroblasts induced by tBHP at a mass concentration of 0.04-1%. p<0.01 ), and there was a dose-effect relationship. The MMP-1 expression inhibition rates of Examples 1-4 were all higher than those of Comparative Examples 1-2, with CI values ​​ranging from 0.2 to 0.4, indicating that the Saururus chinensis extract and the Dendrobium candidum extract synergistically reduced the MMP-1 expression inhibition rate. However, the MMP-1 expression inhibition rates of Comparative Examples 3-11 were also lower than those of Examples 1-4, indicating a significantly insufficient effect in inhibiting MMP-1 expression.

[0095] Table 4 MMP-1 expression inhibition rate and CI value

[0096] Note: NC in the table represents blank control group; tBHP represents tBHP group; ## represents comparison with blank control group p <0.01; * represents comparison with tBHP group p <0.05; ** represents comparison with tBHP group p <0.01.

[0097] The relative expression level of MMP-3, expression inhibition rate and CI value are shown in Table 5. As shown in Table 5, the plant compositions prepared by the preparation processes of Example 1-Example 4 can significantly inhibit the relative expression level of MMP-3 of human fibroblasts induced by tBHP (p<0.01) at a mass concentration of 0.04-1%, and have a dose-effect relationship. The MMP-3 expression inhibition rates of Example 1-Example 4 are higher than those of Comparative Example 1-Comparative Example 2, and the CI values are between 0.25-0.45, indicating that the threeleaf milkwort extract and the dendrobium extract also have a synergistic effect of reducing the MMP-3 expression inhibition rate; and the MMP-1 expression inhibition rates of Comparative Example 3-Comparative Example 11 are lower than those of Example 1-Example 4, and the effect of inhibiting MMP-3 expression is obviously insufficient.

[0098] Table 5 MMP-3 expression inhibition rate and CI value

[0099] Note: NC in the table represents the blank control group; tBHP represents the tBHP group; ## represents compared with the blank control group p <0.01; * represents compared with the tBHP group p <0.05; ** represents compared with the tBHP group p <0.01.

[0100] The above detailed description is a specific description of one of the feasible embodiments of the present application, which is not used to limit the patent scope of the present application. It should be noted that any equivalent implementation or change made without departing from the present application should be included in the scope of the technical scheme of the present application. Therefore, the protection scope of the patent of the present application should be subject to the appended claims.

Claims

1. A preparation process comprising a composite bacterial fermentation process, characterized in that: The preparation process comprises: Mixing 75-90%wt of Saururus chinensis extract with 10-25%wt of Dendrobium officinale extract obtained by enzymatic hydrolysis; or mixing 75-90%wt of Saururus chinensis extract with 10-25%wt of Dendrobium officinale extract obtained by enzymatic hydrolysis; the Saururus chinensis extract is obtained by drying the Saururus chinensis extract, and the Dendrobium officinale extract is obtained by drying the Dendrobium officinale extract; The preparation method of the Saururus chinensis extract comprises the following steps: S1. Preliminary extraction: Extract Saururus chinensis by adding 10-20 times its weight of water, filter, and collect the aqueous extract; then repeat the extraction 0-2 times with the medicinal residue, and combine to obtain a combined aqueous extract; S2, concentration and filtration: The combined aqueous extracts were concentrated to 4-6 times the weight of Saururus chinensis, and filtered to obtain filtrate 1; S3, concentration and sedimentation; filtrate 1 is concentrated to 0.5-2 times the weight of Saururus chinensis, ethanol is added to a final concentration of ethanol of 60-70% v / v, sedimentation is carried out, and the supernatant is collected; S4, decolorization: add 0.5-2%wt activated carbon to the supernatant to decolorize, filter, and obtain filtrate 2; S5, concentration: Filtrate 2 is concentrated to 0.3-0.5 times the weight of Saururus chinensis to obtain Saururus chinensis extract; The method for preparing the dendrobium extract obtained by enzymatic hydrolysis comprises the following steps: (1) Preliminary extraction: Add 50-100 times the weight of water to the dendrobium, filter, and obtain the dendrobium primary extract; (2) Concentration under reduced pressure: The primary extract of Dendrobium officinale is concentrated to 10-20 times the weight of Dendrobium officinale to obtain a concentrated solution; (3) Enzymatic hydrolysis: add 0.01-0.02% w / v medium-temperature amylase to the concentrate and perform enzymatic hydrolysis at 5-10°C for 20-40 min to obtain an enzymatic hydrolyzate; (4) Enzyme removal and impurity removal: The enzymatic hydrolysate is filtered through a 6-20 kD filter membrane to remove macromolecules and enzymes to obtain an ultrafiltrate; (5) Decolorization: Add 0.5-2%wt activated carbon to the ultrafiltrate for decolorization, and filter to obtain a filtrate; (6) Membrane filtration and concentration: The filtrate is filtered through a membrane and concentrated to 0.3-0.5 times the weight of the dendrobium to obtain the dendrobium extract; The mesophilic amylase is obtained through a composite bacterial fermentation process. The preparation method comprises: mixing Bacillus subtilis CMCC(B) 63501 and Bacillus amyloliquefaciens plantarum subspecies BNCC190056 in a live bacterial count ratio of 1:1, inoculating the mixture into a culture medium at a 5% v / v inoculation rate, centrifuging the mixture after fermentation, collecting the supernatant, removing small molecular impurities, collecting the concentrated solution, and purifying the mixture to obtain the mesophilic amylase.

2. The preparation process according to claim 1, characterized in that The extraction conditions in step S1 or step (1) are 90-100° C. for 0.5-1.5 h; Alternatively, the sedimentation condition in step S3 is 20-25° C. for 2-3 hours; Alternatively, the decolorization condition in step S4 or step (5) is 20-30°C for 30-60 min; Alternatively, the filter membrane in step (6) is a 300-400D filter membrane; Alternatively, the fermentation condition is 37±2° C. for 24-48 hours.

3. The plant composition prepared by the preparation process according to any one of claims 1 to 2.

4. Use of the plant composition according to claim 3 in the preparation of anti-aging products, characterized in that: The products include cosmetics or medicines.

5. The use according to claim 4, characterized in that The product contains at least 0.2%wt of the plant composition.

6. A cosmetic, characterized in that: The cosmetic comprises the plant composition according to claim 3.

7. The cosmetic according to claim 6, characterized in that The cosmetics include any one or more of face cream, facial mask, eye cream, neck cream, hand cream, body lotion, moisturizing lotion, essence, toner, shampoo, conditioner, shower gel or cleanser.

8. The cosmetic according to claim 6, characterized in that The cosmetics also include auxiliary materials acceptable in the cosmetics field.

9. A medicine, characterized in that: The medicine comprises the plant composition according to claim 3.

10. The medicine according to claim 9, characterized in that The medicine also includes pharmaceutically acceptable excipients.

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