Microbial enzyme conversion deep processing method for increasing added value of bean dreg waste

Abstract

The invention discloses a microbial enzyme conversion deep processing method for increasing the added value of bean dreg waste, and relates to the technical field of bean dreg treatment.The microbial enzyme conversion deep processing method comprises the following steps that 1, the bean dreg waste is squeezed and dehydrated, and the water content of the bean dreg waste with the water content being 75-80% is reduced to 45-48%; step 2, drying the squeezed and dehydrated bean dregs to reduce the water content to 18-20%; step 3, grinding the dried bean dregs, wherein the particle size of the ground bean dregs is 20-40 meshes; the method is used for carrying out microbial enzyme conversion deep processing on the bean dreg wastes, so that active oligosaccharides and active small peptides in the bean dregs can be effectively extracted, and particularly, soy isoflavone with pharmacological action in the bean dregs can be efficiently extracted. In addition, according to the method for deeply processing the bean dreg waste through microbial enzyme conversion, the bean dreg waste serves as an initial raw material, the increasingly serious solid waste is reduced, and good ecological environment benefits are achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of bean dreg processing, and particularly relates to a microbial enzyme conversion deep processing method for improving the added value of bean dreg waste. BACKGROUND

[0002] Bean dregs are by-products produced during the production of soy milk or tofu, and are rich in nutrients such as protein, dietary fiber, vitamins, and minerals, and also contain beneficial ingredients such as phytosterols. It has various eating methods and can be used to make staple food, dishes, soup and porridge, etc., such as bean dreg steamed buns, fried bean dregs, and bean dreg porridge. It also has other uses such as beauty and skin care, and feed making.

[0003] The existing deep processing of bean dreg waste is based on bean dreg waste as the source, and simple chemical extraction is performed. The existing method has the following disadvantages: 1. It is difficult to achieve a high extraction rate by simply using a chemical extraction method, which leads to the difficulty of achieving economic benefits in the method of chemically extracting active substances from bean dreg waste.

[0004] 2. The existing chemical extraction method requires a large amount of chemical solvent, resulting in high processing cost.

[0005] 3. The existing chemical extraction method will emit a large amount of chemical pollutants during the deep processing of bean dreg waste, causing serious environmental pollution.

[0006] In view of the above problems, the present application provides a microbial enzyme conversion deep processing method for improving the added value of bean dreg waste. SUMMARY

[0007] The present application aims to provide a microbial enzyme conversion deep processing method for improving the added value of bean dreg waste, to solve the problems of low extraction rate, high processing cost, and large pollutant emission of the existing method.

[0008] To achieve the above-mentioned purpose, the present application provides the following technical scheme: A microbial enzyme conversion deep processing method for improving the added value of bean dreg waste, comprising the following steps: Step 1, the bean dreg waste is pressed and dewatered to reduce the water content of the bean dreg waste with a water content of 75-80% to 45-48%; Step 2, the pressed and dewatered bean dregs are dried to reduce the water content to 18-20%; Step 3, the dried bean dregs are ground to a particle size of 20-40 mesh; Step 4, the ground bean dregs are mixed with anhydrous ethanol in a mass ratio of 1:1.5-2 and soaked and stirred; Step 5, centrifugal treatment is carried out on the mixture of the bean dregs obtained in step 4 and anhydrous ethanol, and the supernatant is collected as the raw material for extracting soybean isoflavones; the soybean isoflavones are extracted by using a drying and concentrating technique, and the anhydrous ethanol can be refluxed to step 4 after the extraction of the soybean isoflavones and recycled; Step 6, the precipitate after the centrifugal treatment in step 5 is mixed with water at a ratio of 1:1.5-2, and 0.1-0.15% of mesophilic cellulase is added according to the mass of the precipitate for reaction; after the reaction, 0.15-0.2% of mesophilic hemicellulase is added according to the mass of the precipitate for reaction; after the reaction, centrifugal treatment is carried out, and the supernatant is taken after the treatment; the supernatant is dried and concentrated to obtain oligosaccharides; Step 7, the precipitate after the centrifugal treatment in step 6 is mixed with water at a ratio of 1:1.5-2, and 0.05-0.1% of endoprotease is added for reaction; after the reaction, 0.1-0.15% of exoprotease is added for reaction; after the reaction, centrifugal treatment is carried out, and the supernatant is taken after the centrifugal treatment; the supernatant is dried and concentrated to obtain active small peptides; Step 8, the precipitate after the centrifugal treatment in step 7 is collected and mixed with the pressed water collected in step 1 to serve as the raw material for producing organic foliar fertilizer.

[0009] As a further scheme of the present application, the drying time in step 2 is 2-4 hours, and the drying temperature is 80-90 degrees Celsius.

[0010] As a further scheme of the present application, the soaking and stirring time in step 4 is 10-15 minutes.

[0011] As a further scheme of the present application, the centrifugal treatment time in step 5 is 3-5 minutes, and the centrifugal rotation speed is 4500-5000 revolutions per minute.

[0012] As a further scheme of the present application, the drying and concentrating technical parameters of the soybean isoflavone anhydrous ethanol extract in the bean dregs in step 5 are as follows: the temperature is 70-80 degrees Celsius, and the time is 1.5-2 hours.

[0013] As a further scheme of the present application, the mixing and stirring time of the precipitate with water in step 6 is 120-180 seconds; the reaction conditions of the mesophilic cellulase are as follows: the temperature is in the range of 35-45 degrees Celsius, and the incubation reaction time is 20-30 minutes; The reaction conditions of the mesophilic hemicellulase are as follows: the temperature is in the range of 40-50 degrees Celsius, and the incubation reaction time is 15-20 minutes.

[0014] As a further scheme of the present application: the centrifugal speed in step 6 is 3500-4500 rpm, the centrifugal time is 8-12 minutes, the supernatant concentration temperature is 75-85 DEG C, and the drying concentration time is 5-7 hours.

[0015] As a further scheme of the present application: the mixing time of the precipitate with water in step 7 is 120-180 seconds.

[0016] As a further scheme of the present application: the endoprotease reaction condition in step 7 is that the temperature is 40-50 DEG C, and the incubation time is 15-25 minutes. The exoprotease reaction condition is that the temperature is 35-40 DEG C, and the incubation time is 15-25 minutes.

[0017] As a further scheme of the present application: the centrifugal speed in step 7 is 4500-5000 rpm, the centrifugal time is 10-15 minutes, the supernatant concentration temperature is 70-80 DEG C, and the drying concentration time is 5-6 hours.

[0018] Compared with the prior art, the present application has the following beneficial effects: 1. The present application performs microbial enzyme conversion deep processing on bean dregs waste, and through the high substrate specificity of microbial enzymes, the effective substances such as soybean oligosaccharides and soybean active peptides in the bean dregs can be accurately extracted, so that the extraction rate is effectively improved.

[0019] 2. The present application only uses anhydrous ethanol for extraction, and the anhydrous ethanol can be recycled after the extraction is completed, so that the extraction cost can be effectively reduced.

[0020] 3. The extraction process of the present application uses a variety of microbial enzyme preparations which are environmentally friendly catalysts, and the by-products generated in the separation process can be used as organic fertilizer raw materials, and do not produce new pollution to the environment. The implementation of the present application can effectively utilize the bean dregs waste, and plays a role in absorbing environmental pollution. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 The present application is a process flow diagram. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0023] Please refer to Figure 1The application discloses a microbial enzyme conversion deep processing method for improving the added value of bean dregs waste. Step 1: The bean dregs waste is subjected to squeezing dehydration, so that the water content of the bean dregs waste with a water content of 70% is reduced to 45%.

[0024] Step 2: The squeezed and dehydrated bean dregs are subjected to drying treatment, so that the water content is reduced to below 20%; the drying time is 3 hours, and the drying temperature is 85 DEG C.

[0025] Step 3: The dried bean dregs are subjected to grinding treatment, and the particle size after grinding is 40 mesh.

[0026] Step 4: The ground bean dregs are mixed with anhydrous ethanol according to a mass ratio of 1:1.5, and are subjected to soaking and stirring, and the soaking and stirring time is 10 minutes.

[0027] Step 5: The mixture of the bean dregs and anhydrous ethanol obtained in step 4 is subjected to centrifugal treatment, the centrifugal treatment time is 3 minutes, and the centrifugal speed is 4500 rpm; the supernatant is collected and can be used as a raw material for extracting soybean isoflavones; the soybean isoflavones are extracted by using a drying and concentrating technology, and the anhydrous ethanol can be refluxed to step 4 and recycled after the soybean isoflavones are extracted; the drying and concentrating technology parameters of the soybean isoflavone anhydrous ethanol extract in step 5 are as follows: the temperature is 75 DEG C, and the time is 1.7 hours.

[0028] Step 6: The precipitate after centrifugation in step 5 is mixed with water according to a ratio of 1:1.75, the mixing and stirring time of the precipitate and water is 180 seconds, 0.15% of the precipitate quality is added to the precipitate to react with a medium-temperature cellulase, the medium-temperature cellulase reaction conditions are as follows: the temperature is in the range of 42 DEG C, and the incubation reaction time is 20 minutes; after the reaction, 0.2% of the precipitate quality is added to the precipitate to react with a medium-temperature hemicellulase, the medium-temperature hemicellulase reaction conditions are as follows: the temperature is in the range of 45 DEG C, and the incubation reaction time is 20 minutes; after the reaction, centrifugal treatment is carried out, the centrifugal speed is 4200 rpm, and the centrifugal time is 8 minutes; after the treatment, the supernatant is taken, and the supernatant is dried and concentrated to obtain oligosaccharides; the supernatant concentration temperature is 80 DEG C, and the drying and concentrating time is 6 hours; the obtained oligosaccharides can be used as a raw material for producing pet functional oligosaccharide food.

[0029] Step 7, the precipitate after centrifugation in step 6 is mixed with water in a ratio of 1:2, the mixing time of the precipitate and water is 120 seconds, endoprotease is added in an amount of 0.1% of the mass of the precipitate for reaction, the reaction conditions of the endoprotease are: temperature of 45 degrees Celsius, reaction time of 20 minutes; after reaction, exoprotease is added in an amount of 0.15% of the mass of the precipitate for reaction, the reaction conditions of the exoprotease are: temperature of 38 degrees Celsius, reaction time of 15 minutes; after reaction, centrifugation is performed, the centrifugation speed is 5000 revolutions per minute, the centrifugation time is 12 minutes, after centrifugation, the supernatant is taken, the supernatant is dried and concentrated to obtain active small peptides, the concentration temperature of the supernatant is 75 degrees Celsius, the drying and concentration time is 5 hours, the active small peptides can be used as raw materials for producing functional active small peptide food for pets.

[0030] Step 8, the precipitate after centrifugation in step 7 is collected and mixed with the press water collected in step 1 to serve as raw materials for producing organic foliar fertilizer.

[0031] Using the method to perform microbial enzyme conversion deep processing on bean dregs waste, in addition to being able to effectively extract active oligosaccharides and active small peptides in the bean dregs, especially being able to efficiently extract soybean isoflavones with pharmacological effects in the bean dregs. In addition, the method of performing microbial enzyme conversion deep processing on bean dregs waste takes the bean dregs waste as the initial raw material, has a reduction effect on the increasingly serious solid waste, and has good ecological environmental benefits.

[0032] It is apparent to those skilled in the art that the present application is not limited to the details of the foregoing exemplary embodiments, and that the present application can be implemented in other specific forms without departing from the spirit or essential characteristics of the present application. Although the present specification is described in terms of embodiments, not every embodiment need contain only one technical solution, and the description of the specification is for the sake of clarity, and those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can be appropriately combined to form other embodiments that those skilled in the art can understand.

Claims

1. A microbial enzyme conversion deep processing method for increasing the added value of bean dregs waste, characterized by: The following steps are involved: Step 1: Squeeze and dehydrate the bean dregs waste to reduce the moisture content of the bean dregs waste from 75-80% to 45-48%; Step 2: Dry the squeezed and dehydrated bean dregs to reduce the moisture content to 18-20%; Step 3: Grinding the dried bean dregs to a particle size of 20-40 mesh; Step 4: Mix the ground bean dregs with anhydrous ethanol in a mass ratio of 1:1.5-2, and soak and stir; Step 5: centrifuging the mixture of the dregs and anhydrous ethanol obtained in step 4, collecting the supernatant and using it as a raw material for extracting soy isoflavones. The soy isoflavones are extracted using a drying and concentration technique, and the anhydrous ethanol can be refluxed to step 4 after the soy isoflavones are extracted and recycled. Step 6: The precipitate after centrifugation in step 5 is mixed with water at a ratio of 1:1.5-2, and a medium-temperature cellulase is added according to 0.1-0.15% of the mass of the precipitate to react. After the reaction, a medium-temperature hemicellulase is added according to 0.15-0.2% of the mass of the precipitate to react. After the reaction, the reaction is centrifuged, and the supernatant is collected after the treatment, and the supernatant is dried and concentrated to obtain oligosaccharides; Step 7, the precipitate after centrifugation in step 6 is mixed with water at a ratio of 1:1.5-2, 0.05-0.1% of the mass of the precipitate is added with endoprotease for reaction, and after the reaction, 0.1-0.15% of the mass of the precipitate is added with exoprotease for reaction, and the reaction is centrifuged after the reaction. After the centrifugation, the supernatant is collected, and the supernatant is dried and concentrated to obtain the active small peptide; Step 8: The precipitate obtained after centrifugation in step 7 is collected and mixed with the squeezed water collected in step 1 to be used as a raw material for producing organic foliar fertilizer.

2. The method for deep processing of bean dregs by microbial enzyme conversion for increasing the added value of bean dregs waste according to claim 1, characterized in that: The drying time in step 2 is 2-4 hours, and the drying temperature is 80-90 degrees Celsius.

3. The method for deep processing of bean dregs by microbial enzyme conversion for increasing the added value of bean dregs waste according to claim 1, characterized in that: The soaking and stirring time in step 4 is 10-15 minutes.

4. The method for deep processing of bean dregs by microbial enzyme conversion for increasing the added value of bean dregs waste according to claim 1, characterized in that: The centrifugal treatment time in step 5 is 3-5 minutes, and the centrifugal speed is 4500-5000 revolutions per minute.

5. The method for deep processing of bean dregs by microbial enzyme conversion for increasing the added value of bean dregs waste according to claim 1, characterized in that: The technical parameters for drying and concentrating the anhydrous ethanol extract of soy isoflavones in the bean dregs in step 5 are: temperature of 70-80 degrees Celsius and time of 1.5-2 hours.

6. The method for deep processing of bean dregs by microbial enzyme conversion for increasing the added value of bean dregs waste according to claim 1, characterized in that: In step 6, the mixing time of the precipitate and water is 120-180 seconds, and the medium-temperature cellulase reaction conditions are: the temperature is in the range of 35-45 degrees Celsius, and the insulation reaction time is 20-30 minutes; The mesophilic hemicellulase reaction conditions are: a temperature in the range of 40-50 degrees Celsius and a heat preservation reaction time of 15-20 minutes.

7. The method for deep processing of bean dregs by microbial enzyme conversion for increasing the added value of bean dregs waste according to claim 1, characterized in that: In step 6, the centrifugal speed is 3500-4500 rpm, the centrifugal time is 8-12 minutes, the supernatant concentration temperature is 75-85 degrees Celsius, and the drying and concentration time is 5-7 hours.

8. The method for deep processing of bean dregs by microbial enzyme conversion for increasing the added value of bean dregs waste according to claim 1, characterized in that: The precipitate in step 7 is added to water and mixed for 120-180 seconds.

9. The method for deep processing of bean dregs by microbial enzyme conversion for increasing the added value of bean dregs waste according to claim 1, characterized in that: The endoprotease reaction conditions in step 7 are: a temperature of 40-50 degrees Celsius and a heat-insulating reaction time of 15-25 minutes; The exoprotease reaction conditions are: temperature of 35-40 degrees Celsius, and insulation reaction time of 15-25 minutes.

10. The method for deep processing of bean dregs by microbial enzyme conversion for increasing the added value of bean dregs waste according to claim 1, characterized in that: In step 7, the centrifugal speed is 4500-5000 rpm, the centrifugal time is 10-15 minutes, the supernatant concentration temperature is 70-80 degrees Celsius, and the drying and concentration time is 5-6 hours.

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