Composite micro-ecological inducer as well as preparation method and use method thereof

Abstract

The invention relates to a composite micro-ecological inducer as well as a preparation method and a use method thereof. The compound micro-ecological inducer comprises an inducing factor, a metabolic regulation factor, a micro-ecological stabilization factor, a low-temperature adaptation factor, nutritional ingredients and a carrier, anaerobic ammonium oxidation bacteria can be rapidly and stably induced to react only through additional dosing under the condition that equipment transformation is not carried out, short nitrification and denitrification reaction paths are constructed in a synergistic manner, and the yield of the anaerobic ammonium oxidation bacteria is increased. The denitrification efficiency is maximized; the activity of the Anammox bacteria can be induced and enhanced within 3-7 days, and a long-term domestication process is avoided; the micro-ecological stability in a sewage treatment system is improved by selectively inhibiting proliferation of infectious microbes or competitive strains; the denitrification reaction can still be continuously carried out in a low-temperature environment, so that the operation stability of the sewage treatment system in winter and cold regions is ensured; and by controlling the structure of the microbial community, the dominant position of the dominant flora is ensured, and the reproducibility and reliability of the process are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of sewage treatment, and in particular to a composite microecological inducer and a preparation method and a use method thereof. Background Art

[0002] Among traditional wastewater treatment processes for nitrogen removal, nitrification-denitrification is widely used. However, this process has the disadvantages of requiring a large amount of organic carbon source and consuming a lot of energy. Particularly in cold regions or under low temperatures in winter, the activity of nitrifying and denitrifying bacteria is significantly reduced, leading to a significant decrease in treatment efficiency.

[0003] Among them, the anaerobic ammonium oxidation (Anammox) reaction directly converts ammonia (NH4 + ) and nitrous acid (NO2 - ) is converted into nitrogen (N2) by a reaction that requires virtually no carbon source or aeration energy. Therefore, it is considered a next-generation denitrification technology and is attracting considerable attention. However, Anammox bacteria are highly sensitive to their environment, and the reaction requires weeks to months to start. This drawback makes it difficult to directly apply to existing processes such as activated sludge or A² / O processes.

[0004] In addition, a low-cost nitrogen removal process using shortcut nitrification-denitrification (SND) has also been proposed, but it also faces the difficulties of microbial community control and pH and ORP optimization, making it difficult to achieve stable NO2 - accumulation.

[0005] Based on the above background, there is an urgent need to develop an external biological agent that does not require the modification of existing equipment, can regulate the microbial community structure externally, induce the Anammox reaction in a short period of time, and synergistically promote short-range denitrification.

[0006] In recent years, research on Anammox technology has made progress, and some external biological agents and microbial enhancers have been proposed. For example, Japanese Patent Publication No. 2023-128215 discloses a denitrification preparation of microbial communities and their carrier materials for promoting anaerobic ammonia oxidation reactions, but these preparations are mainly targeted at fixed-bed reactors and have poor performance in adapting to existing equipment. In addition, the activation of the bacterial community still requires a long acclimatization process (more than 2 to 4 weeks), and its on-site applicability is limited. In addition, there are also some enzyme regulators or organic additives that promote short-range nitrification, but in most cases, NO2 - Accumulation is unstable, and synergistic control of Anammox activity has not been successfully achieved. In addition, there are very limited formulations that can operate stably at low temperatures (below 12°C). Summary of the Invention

[0007] Based on this, it is necessary to provide a composite microecological inducer that has high low-temperature adaptability and can take effect quickly without modifying existing equipment, as well as a preparation method and use method thereof.

[0008] A composite microecological inducer comprises the following components, calculated by mass percentage: 5% to 15% of an induction factor, 10% to 25% of a metabolic regulating factor, 1% to 10% of a microecological stabilization factor, 5% to 15% of a low temperature adaptation factor, and the remainder of nutrients and a carrier.

[0009] In one embodiment, the following components are included by mass percentage: 10% induction factors, 15% metabolic regulatory factors, 5% microecological stabilization factors, 10% low temperature adaptation factors, and 60% nutrients and carriers.

[0010] In one embodiment, the induction factors include β-glucan, biological signal peptides and short-chain fatty acid salts.

[0011] In one embodiment, the metabolic regulator includes NAD + / NADH metabolism regulator, carbon flow regulator and electron acceptor regulatory component.

[0012] In one embodiment, the microecological stabilizing factors include lactoferrin peptide, chitosan oligosaccharide and biofilm regulator.

[0013] In one embodiment, the low temperature adaptation factor includes a class of psychrophilic microorganisms and a cryoprotectant.

[0014] In one embodiment, the nutrients include nitrogen source, phosphorus source, potassium source and trace elements.

[0015] In one embodiment, the nutritional ingredients further include protease, amylase, and cellulase.

[0016] In one embodiment, the carrier is a liquid preparation with good biocompatibility or a mixture of particulate matter with a high specific surface area and a liquid with good biocompatibility.

[0017] The above-mentioned composite microecological inducer is prepared by combining an induction factor for rapidly inducing the target functional bacteria (anaerobic ammonia-oxidizing bacteria, short-term nitrifying bacteria, etc.) in the sewage to be treated to express key metabolic enzymes, a metabolic regulatory factor for promoting synergistic reactions in heterotrophic denitrification and autotrophic nitrogen conversion, a microecological stabilization factor for maintaining the stable enrichment of key functional bacteria (such as Anammox bacteria and denitrifying bacteria) in the sewage treatment system, a low-temperature adaptation factor for improving the metabolism and enzymatic activity of the flora in a low-temperature environment (5°C to 15°C), and nutrients and carriers for providing the flora with the nutrients required for basal metabolism according to preset proportions. The composite microecological inducer is used to be added to the sewage to be treated in the sewage treatment system to efficiently remove nitrogen components in urban domestic sewage and high-nitrogen industrial wastewater, and achieve the reduction of carbon sources and electricity and the improvement of treatment stability through the synergistic effect of rapid activation of anaerobic ammonia-oxidizing bacteria (Anammox bacteria) and short-term denitrification.

[0018] Therefore, the above-mentioned composite microecological inducer can quickly and stably induce anaerobic ammonia-oxidizing bacteria reactions by only adding external drugs without modifying the equipment of the existing sewage treatment system, and maximize the denitrification efficiency by synergistically constructing short nitrification and denitrification reaction pathways; experimental verification has proved that it can induce and enhance the activity of Anammox bacteria within 3 to 7 days. Compared with the 2-4 weeks of acclimation period required in traditional sewage treatment technology, the above-mentioned composite microecological inducer can avoid the long-term acclimation process and improve the sewage treatment efficiency; by selectively inhibiting the proliferation of miscellaneous bacteria or competing strains, the microecological stability of the sewage treatment system is improved; by being able to continue the denitrification reaction in a low temperature environment, the operational stability of the sewage treatment system in winter and cold areas is ensured; by controlling the structure of the microbial community, the dominant position of the dominant bacteria (Anammox bacteria, short-range denitrifying bacteria, etc.) is ensured, and the reproducibility and reliability of the process are improved.

[0019] A method for preparing a composite microecological inducer comprises the steps of: Mix 5% to 15% by weight of an induction factor, 10% to 25% by weight of a metabolic regulation factor, 1% to 10% by weight of a microecological stabilization factor, 5% to 15% by weight of a low-temperature adaptation factor, and the remaining nutrients and a carrier, and adjust the pH value of the mixed preparation to 6.5 to 7.5; filtering out miscellaneous bacteria in the mixed preparation to obtain a composite microecological inducer; The composite microecological inducer is placed in a light-proof container and sealed and refrigerated.

[0020] The preparation method of the above-mentioned composite microecological inducer can obtain a composite microecological inducer that is protected from light and stored in a refrigerated state.

[0021] The application discloses a method for using a composite micro-ecological inducer, and belongs to the technical field of wastewater treatment. The adding mode is direct adding, dilution adding or automatic quantitative adding, the adding frequency is once every 1-2 months, and the adding amount is 0.1wt%-0.5wt% of the chemical oxygen demand of the wastewater to be treated.

[0022] The method for using the composite micro-ecological inducer can effectively, quickly and completely complete the wastewater treatment of urban wastewater, industrial wastewater and the like without modifying the equipment of the traditional wastewater treatment system. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 The figure is a flowchart of a preparation method of the composite micro-ecological inducer in the preferred embodiment of the application. DETAILED DESCRIPTION

[0024] In order to facilitate the understanding of the application, the application will be described in detail below with reference to the relevant drawings. The drawings show the preferred embodiments of the application. However, the application can be realized in many different forms, and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the application more thorough and comprehensive.

[0025] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used in the description of the application herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used herein, the term "and / or" includes any and all combinations of one or more of the associated listed items.

[0026] In describing a position relationship, unless otherwise specified, when an element is referred to as "on" another element, it can be directly on the other element or there can be an intermediate element. It can also be understood that when an element is referred to as "between" two elements, it can be the only one between the two elements, or there can be one or more intermediate elements.

[0027] In the case of using "include", "have", and "contain" described herein, unless the explicit limiting term such as "only", "consisting of", etc. is used, another component can also be added. Unless otherwise mentioned, the singular form of the term can include the plural form, and cannot be understood as the number of one.

[0028] The composite microecological inducer of a preferred embodiment of the present invention is suitable for treating municipal and industrial wastewater, particularly wastewater with high nitrogen concentrations, such as dyeing wastewater, food wastewater, and livestock wastewater. The composite microecological inducer comprises the following components, by weight: 5% to 15% of an induction factor, 10% to 25% of a metabolic regulator, 1% to 10% of a microecological stabilization factor, 5% to 15% of a low-temperature adaptation factor, and the remainder of nutrients and a carrier.

[0029] Specifically, the induction factors include β-glucan, biological signal peptides and short-chain fatty acid salts, which are used to quickly induce functional bacteria (including anaerobic ammonia oxidizing bacteria, short-range nitrifying bacteria, etc.) to express pipe metabolic enzymes, accelerate the startup of the sewage treatment system and improve the overall reaction rate of sewage treatment.

[0030] Specifically, metabolic regulators include NAD + / NADH metabolic regulators, carbon flow regulators and electron acceptor regulatory components are used to optimize the carbon-nitrogen metabolic pathways and electron flow transfer efficiency among bacterial communities, and promote the synergistic reaction of heterotrophic denitrification and autotrophic nitrogen conversion processes. + The NADH metabolism regulator can be nicotinamide, etc., and the carbon flow regulator can be sodium fumarate, sodium citrate, etc.

[0031] Specifically, microecological stabilization factors include lactoferrin peptide, chitosan oligosaccharides and biofilm regulators, which are used to selectively inhibit the proliferation of miscellaneous bacteria or competing strains, maintain the stable enrichment of key functional bacteria (such as Anammox bacteria, denitrifying bacteria, etc.) in the sewage treatment system, and improve the system's microecological stability.

[0032] Specifically, low-temperature adaptation factors include a class of psychrophilic microorganisms and cryoprotectants, which are used to enhance the metabolic and enzymatic activity of the bacterial community in low-temperature environments (5°C to 15°C), thereby ensuring the stable operation of the system in winter or at high latitudes. The psychrophilic microorganisms may include psychrophilic Candida, psychrophilic Trichoderma, psychrophilic Aspergillus niger, etc., and the cryoprotectants may include glycerol, trehalose, membrane stabilizers, etc.

[0033] Specifically, nutrients include nitrogen, phosphorus, potassium, and trace elements, providing the essential nutrients for basal metabolism. Nitrogen sources can include urea, ammonium sulfate, and potassium nitrate; phosphorus sources can include potassium dihydrogen phosphate and disodium hydrogen phosphate; potassium sources can include potassium chloride and potassium sulfate; and trace elements include Fe, Zn, Cu, and Mn. Nitrogen, phosphorus, and potassium sources serve as substrates, while trace elements support the cofactor requirements of key metabolic enzymes.

[0034] In order to further enhance the substrate degradation ability of the composite microecological inducer on complex organic pollutants and the metabolic response rate of the core bacterial community, more specifically, the nutritional components also include protease, amylase, and cellulase.

[0035] Among them, protease is used to accelerate the hydrolysis of protein-like organic matter in sewage and improve the effective utilization of organic nitrogen and carbon sources by heterotrophic bacteria; amylase is used to promote the degradation of polysaccharides (such as starch, plant polysaccharides, etc.), release short-chain absorbable carbon sources, and help activate the metabolic pathways of short-range denitrifying bacteria; cellulase is used to break down residual cellulose structures in sewage and carriers, expand the types of carbon sources, and enhance the system's carbon-nitrogen synergistic conversion potential.

[0036] Protease, amylase and cellulase are all enzyme preparations of natural origin. Protease is a natural protein hydrolase, amylase is extracted from plants or microorganisms, and cellulase is also of natural origin, generally produced by bacteria, fungi, etc.

[0037] Therefore, the aforementioned functional enzymes and other nutrients are combined in a synergistic manner to form an enzymatic-nutrient complex unit with the functions of "substrate pre-degradation and metabolic acceleration." This unit can significantly optimize the metabolic environment during system startup, shortening the activation time of anaerobic ammonia-oxidizing bacteria (Anammox bacteria) and short-range denitrifying bacteria, while also improving the system's adaptability and stability under low temperature, high load, and complex organic pollution conditions. In some embodiments, the carrier comprises a liquid preparation with good biocompatibility or a mixture of high-surface-area particulate matter and a liquid with good biocompatibility.

[0038] Specifically, the carrier is a liquid preparation with good biocompatibility or a mixture formed by adding high-specific-surface-area particulate matter to a liquid preparation with good biocompatibility, used to carry nutrients and maintain their biological activity. The carrier is a liquid system primarily used to carry nutrients and maintain their biological activity; the liquid preparation with good biocompatibility can be one or a mixture of buffer, diluent, and stabilizing liquid; the high-specific-surface-area material can be volcanic mineral powder, diatomaceous earth, porous biochar, or sodium alginate-encapsulated particles.

[0039] The above-mentioned composite microecological inducer is prepared by configuring the induction factor, metabolic regulatory factor, microecological stabilization factor, low-temperature adaptation factor, nutrients and carrier according to the above-mentioned proportions, and is used to be added to the untreated sewage in the sewage treatment system to efficiently remove the nitrogen components in urban domestic sewage and high-nitrogen industrial wastewater. Through the synergistic effect of the rapid activation of anaerobic ammonia-oxidizing bacteria (Anammox bacteria) and short-range denitrification, the carbon source and electricity are reduced and the treatment stability is improved.

[0040] Therefore, the above-mentioned composite microecological inducer can quickly and stably induce anaerobic ammonia-oxidizing bacteria reactions by only adding external drugs without modifying the equipment of the existing sewage treatment system, and maximize the denitrification efficiency by synergistically constructing short nitrification and denitrification reaction pathways; experimental verification has proved that it can induce and enhance the activity of Anammox bacteria within 3 to 7 days. Compared with the acclimation period of 2 to 4 weeks required in traditional sewage treatment technology, the above-mentioned composite microecological inducer can avoid the long-term acclimation process and improve the sewage treatment efficiency; by selectively inhibiting the proliferation of miscellaneous bacteria or competing strains, the microecological stability in the sewage treatment system is improved; by being able to continue the denitrification reaction in a low temperature environment, the operational stability of the sewage treatment system in winter and cold areas is ensured; by controlling the structure of the microbial community, the dominant position of the dominant bacteria (Anammox bacteria, short-range denitrifying bacteria, etc.) is ensured, and the reproducibility and reliability of the process are improved.

[0041] Therefore, the composite microecological inducer can be used directly in existing sewage treatment systems without the need for equipment modification, helping to reduce sewage treatment costs. It can also quickly start sewage treatment, improve sewage treatment efficiency, and significantly increase the amount of sewage treated in a given period. Furthermore, it is highly practical for sewage treatment in cold regions and in winter, where treatment efficiency decreases significantly. Furthermore, the composite microecological inducer is also suitable for sewage treatment at conventional temperatures (15°C to 50°C), demonstrating its high applicability.

[0042] In order to more intuitively understand the effect of the above-mentioned composite microecological inducer, a test was conducted in an actual sewage treatment facility to verify the following: In a real sewage treatment facility, the Anammox method is used for denitrification. The effective capacity of the anaerobic tank is 50m³. The raw water quality analysis results show that the chemical oxygen demand (COD) is 400 mg / L and the ammonia nitrogen (NH4 + The composite microecological inducer of the present invention is added at a ratio of 1.0 wt% of the COD amount (ie, about 200 g or 200 mL per day).

[0043] After adding the above-mentioned composite microecological inducer, it was confirmed that the following treatment performances were improved: COD removal rate increased by 20% compared with before addition, and the removal performance of organic matter in the treated water was greatly improved; ammonia nitrogen (NH4 + The removal rate of nitrogen (N) increased by 30% and total nitrogen (TN) by 25%, increasing overall denitrification efficiency. Furthermore, the addition of the aforementioned composite microbial inducer improved the operational stability of the sewage treatment system and significantly suppressed the generation of excess sludge. Specifically, the activated sludge concentration (MLSS) dropped from 4500mg / L before treatment to 2100mg / L after treatment, achieving a sludge reduction of over 50%.

[0044] In addition, in addition to improving the treated water quality after addition, the significant reduction in the amount of residual sludge generated also helps to reduce operating costs such as sludge treatment, transportation and incineration, as well as carbon dioxide emissions.

[0045] In some embodiments, the composite microecological inducer comprises the following components by mass percentage: 10% induction factors, 15% metabolic regulatory factors, 5% microecological stabilization factors, 10% low temperature adaptation factors, and 60% nutrients and carriers.

[0046] In order to further clarify the various properties of the above-mentioned composite microecological inducer, the following basic performance evaluations at the laboratory level were performed: First, a model synthetic wastewater (COD 400 mg / L, NH4 + -N 50mg / L, NO3 - 20mg / L) for treatment performance evaluation; Secondly, a composite microecological inducer prepared by using the above-mentioned components in corresponding proportions was added to the above-mentioned synthetic wastewater for testing.

[0047] Compared with the control group (no addition), the experimental group added with compound microecological inducer confirmed the following effects: COD removal rate increased from 72% to 90%; NH4 + -N removal rate increased from 54% to 85%; total nitrogen (TN) removal rate increased from 43% to 68%; NO2 in treated water - The concentration increased rapidly, NO3 - The concentration dropped to less than one-third; starting on the third day of sewage treatment, HzsA gene expression surged, reaching 49 times that of the untreated group on the fifth day, and Hzo gene expression reached 5.6 times. This confirms that the composite microecological inducer can effectively induce and activate the Anammox reaction in a short period of time and under low temperature conditions, and achieve a synergistic process with short-range denitrification.

[0048] The present invention also provides a method for preparing a composite microecological inducer, comprising steps S10 to S30.

[0049] Step S10: Mix 5% to 15% by weight of the induction factor, 10% to 25% by weight of the metabolic regulator, 1% to 10% by weight of the microecological stabilization factor, 5% to 15% by weight of the low-temperature adaptation factor, the remaining nutrients and the carrier, and adjust the pH value of the mixed preparation to 6.5 to 7.5. Specifically, the number of viable bacteria in the mixed preparation is ≥10 8 CFU / mL.

[0050] Step S20, filtering out miscellaneous bacteria in the mixed preparation to obtain the composite micro-ecological inducer.

[0051] Specifically, the mixed preparation is filtered by using a 0.22 μm membrane to remove miscellaneous bacteria in the mixed preparation.

[0052] Step S30, the composite micro-ecological inducer is filled into a light-proof container and sealed for refrigeration.

[0053] Specifically, the composite micro-ecological inducer is filled into a light-proof container and sealed for refrigeration at 0℃-8℃. More specifically, the temperature of the storage environment is 4℃.

[0054] By performing step S10, the mixed preparation with neutral pH is obtained. By performing step S20, the composite micro-ecological inducer with less or no miscellaneous bacteria is obtained. By performing step S30, the prepared composite micro-ecological inducer is properly stored to ensure the biological activity of the composite micro-ecological inducer. Therefore, by performing steps S10-S20, the composite micro-ecological inducer stored in light-proof and refrigeration is prepared.

[0055] The application also provides a method for using the composite micro-ecological inducer, which is added into sewage to be treated in a traditional sewage treatment system for sewage treatment. The adding method is direct adding, dilution adding or automatic quantitative adding, the adding frequency is once every 1-2 months, and the adding amount is 0.1wt%-0.5wt% of the chemical oxygen demand of the sewage to be treated. Specifically, the composite micro-ecological inducer is added into sewage to be treated in a traditional sewage treatment system using the activated sludge method for sewage treatment.

[0056] By using the method for using the composite micro-ecological inducer, the sewage treatment of urban wastewater, industrial wastewater and the like can be effectively, quickly and completely completed without equipment modification of the traditional sewage treatment system.

[0057] The technical features of the above-described embodiments can be combined in any manner. To make the description concise, all possible combinations of the technical features in the above-described embodiments are not described, but as long as the combinations of the technical features do not contradict, they should be considered within the scope of the present application.

[0058] The above-described embodiments only express several embodiments of the present application, and the description is relatively specific and detailed, but it should not be understood as a limitation on the scope of the patent. It should be noted that for ordinary skilled in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which are within the scope of the present application. Therefore, the protection scope of the present application should be subject to the appended claims.

Claims

1. A composite microecological inducer, characterized in that: Calculated by mass percentage, it includes the following components: 5% to 15% of induction factors, 10% to 25% of metabolic regulatory factors, 1% to 10% of microecological stabilization factors, 5% to 15% of low temperature adaptation factors, and the remainder of nutrients and carriers.

2. The composite microecological inducer according to claim 1, characterized in that Calculated by mass percentage, it includes the following components: 10% induction factors, 15% metabolic regulatory factors, 5% microecological stabilization factors, 10% low temperature adaptation factors, and 60% nutrients and carriers.

3. The composite microecological inducer according to claim 1, characterized in that The induction factors include beta-glucan, biological signal peptide and short-chain fatty acid salt.

4. The composite microecological inducer according to claim 1, characterized in that The metabolic regulators include NAD + / NADH metabolism regulator, carbon flow regulator and electron acceptor regulatory component.

5. The composite microecological inducer according to claim 1, characterized in that The microecological stabilizing factors include lactoferrin peptide, chitosan oligosaccharide and biofilm regulator.

6. The composite microecological inducer according to claim 1, characterized in that The low temperature adaptation factors include a class of psychrophilic microorganisms and low temperature protectants.

7. The composite microecological inducer according to claim 1, characterized in that The nutrient components include nitrogen source, phosphorus source, potassium source and trace elements.

8. The composite microecological inducer according to claim 7, characterized in that The nutritional ingredients also include protease, amylase and cellulase.

9. The composite microecological inducer according to claim 1, characterized in that The carrier is a liquid preparation with good biocompatibility or a mixed liquid formed by mixing granular materials with high specific surface area and liquid with good biocompatibility.

10. A method for preparing a composite microecological inducer, characterized in that: Including steps: Mix 5% to 15% by weight of an induction factor, 10% to 25% by weight of a metabolic regulation factor, 1% to 10% by weight of a microecological stabilization factor, 5% to 15% by weight of a low-temperature adaptation factor, and the remaining nutrients and a carrier, and adjust the pH value of the mixed preparation to 6.5 to 7.5; filtering out miscellaneous bacteria in the mixed preparation to obtain a composite microecological inducer; The composite microecological inducer is placed in a light-proof container and sealed and refrigerated.

11. A method for using a composite microecological inducer, characterized in that: Adding the composite microecological inducer according to any one of claims 1 to 9 to the untreated sewage in a traditional sewage treatment system for sewage treatment; The addition method is direct addition, dilution addition or automatic quantitative addition, the addition frequency is once every 1 to 2 months, and the addition amount is 0.1wt% to 0.5wt% of the chemical oxygen demand of the sewage to be treated.

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