Extraction method of high-polarity micrococcus oil and application of high-polarity micrococcus oil

Abstract

The invention discloses an extraction method and application of high-polarity micrococcus oil, and relates to the technical field of application of algae. The invention discloses an extraction method and application of micrococcus high-polarity oil. The extraction method comprises the steps of extraction of micrococcus oil, purification of the micrococcus oil and application of the micrococcus high-polarity oil. According to the application, the high-polarity micrococcus faecalis oil is obtained by extracting and purifying the micrococcus faecalis powder, is not influenced by marine pollution and gradual exhaustion of fishery due to excessive fishing, and is more friendly to consumers allergic to fishes or shellfishes; the polar lipid in the micrococcus has unique biomedical activity, for example, the polar lipid of the micrococcus can be self-emulsified in the digestive tract, and the level of triglyceride, cholesterol and low-density lipoprotein released into blood is reduced; the polar lipid of the micrococcus can help DHA and other fatty acids to efficiently cross the blood brain barrier, and the DHA and other fatty acids are sent to the brain; the betaine ester of the micrococcus is specifically combined with paraoxonase 1, so that the atherosclerosis is prevented and reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of algae applications, and in particular to a method for extracting high-polarity oil from Nannochloropsis spp. and applications thereof. Background Art

[0002] Omega-3 PUFAs are both nutrients and essential fatty acids, crucial for our health and well-being. EPA (eicosapentaenoic acid) is a widely used omega-3 unsaturated fatty acid with significant physiological significance in nutrition and medicine. It plays a positive role in the prevention and treatment of diseases such as rheumatoid arthritis, hypertension, and diabetes. It is also a nutritional supplement used to assist in the treatment of COVID-19. It has anti-inflammatory, anti-aging, anti-coagulation, and immune system regulation properties, and can be used as an adjunct to the treatment of a variety of clinical conditions, including cancer, skin diseases, and geriatric conditions. The FDA has approved the highly purified fish oil preparation Vascepa (eicosapentaenoic acid ethyl ester, EPA-EE) for reducing the risk of cardiovascular events. Chinese consumers spend only $0.26 per capita on omega-3 supplements, suggesting significant potential demand for EPA products in China.

[0003] my country has a complete "fishery + omega-3 fatty acid raw material extraction + deep processing" industry chain. Omega-3 fatty acids (DHA and EPA) are extracted from the fishery, then processed by processing companies for use in dietary supplements, food and beverages, infant food, pharmaceuticals, and clinical nutrition. Currently, EPA primarily comes from marine fish, but it's difficult to completely separate EPA and DHA from fish oil. Furthermore, with global fishery resources declining annually, relying solely on fish oil as a source of omega-3s is far from sufficient to meet market demand. Furthermore, fish oil EPA resources are gradually being depleted due to marine pollution and overfishing, impacting ecological balance. Furthermore, the fish oil product Vascepa should be used with caution in patients with hypersensitivity to fish and / or shellfish. Summary of the Invention

[0004] The present invention aims to provide a method for extracting highly polar oil from Nannochloropsis spp. and its application, so as to solve the problems that EPA and DHA in fish oil are difficult to completely separate, fish oil EPA resources are gradually depleted due to marine pollution and overfishing, and fish oil products still require caution in use by consumers who are hypersensitive to fish and / or shellfish.

[0005] In order to achieve the above object, the present invention provides the following technical solutions: A method for extracting highly polar oil from Microchloropsis spp. comprises the following steps: extracting the oil from the microchloropsis spp., purifying the oil from the microchloropsis spp., S1. Algal oil extraction: Nannochloropsis algae powder is contacted with ethanol while heated to 50-70 degrees Celsius, and then the filtrate is collected by centrifugation and filtration to obtain a crude algal oil extract containing ethanol; S2. Algae Oil Purification: The crude ethanolic algae oil extract is placed in a cooling tank for winterization. After winterization, the residue is filtered to remove high-melting-point fatty acid esters and other waxy impurities from the crude algae oil extract. The filtrate is collected and transferred to a filtrate tank. Further evaporation, washing, and concentration of the filtrate yield highly polar algae oil P1 with significantly reduced impurities.

[0006] Furthermore, the ethanol in the ethanol-containing crude algae oil extract in S1 is evaporated to an ethanol content below 2000 ppm to obtain a paste-like crude algae oil, in which polar lipids account for 45-70% of the total lipids, and the polar lipids include a phospholipid lipid composition, a glycolipid lipid composition and a betaine lipid lipid composition; the content of total fatty acids in the crude algae oil is 30g-45g / 100g, and the content of eicosapentaenoic acid in the crude algae oil is 10g-17g / 100g.

[0007] Furthermore, the crude algae oil paste is heated to 55-65 degrees Celsius and brought into contact with ethanol; placing the mixture of the crude algae oil and ethanol in a cooling tank to winterize the crude algae oil mixture containing ethanol by lowering the temperature; After winterization is complete, the residue is separated by filtration, and the filtrate phase is collected and put into a filtrate tank. The filtrate is further evaporated, washed with water, and concentrated to obtain high-polarity algae oil P2.

[0008] Furthermore, the high-polarity algae oil P1 or the high-polarity algae oil P2 contained 1.5 wt% to 30 wt% of eicosapentaenoic acid and 50 wt% to 90 wt% of polar lipids, and the polar lipids by column chromatography were 56.47-82.30%; Polar lipids include phospholipid conjugates, glycolipid conjugates, betaine lipid conjugates, triglyceride conjugates, or diglyceride conjugates; Among them, 3wt%~20wt% of lipids are phospholipid conjugates, and column chromatography is 1.15-12.54%. Among them, 10wt%~35wt% of the lipids are glycolipid conjugates, and the column chromatography is 14.20-26.77%, Among them, 3wt%~15wt% of the lipids are betaine lipid conjugates, and the column chromatography is 5.77-11.91%, Among them, 3wt%~25wt% of the lipids are triglyceride conjugates or diglyceride conjugates, and the column chromatography is 6.68-16.81%.

[0009] Furthermore, the content of total fatty acids in the high-polarity algae oil P1 or high-polarity algae oil P2 in the high-polarity algae oil is 40-60g / 100g, the content of eicosapentaenoic acid in the high-polarity algae oil is 15g-30g / 100g, and the proportion of polar lipids in the total lipids is 50-90%.

[0010] Furthermore, the polar lipids of the high-polarity algae oil P1 or the high-polarity algae oil P2 include a phospholipid lipid composition, a glycolipid lipid composition, and a betaine lipid lipid composition; In the total lipids, 3w% to 25wt% of the lipids are phospholipid lipid compositions, wherein the phospholipid lipid compositions include, but are not limited to, phosphatidylcholine (PC), phosphatidylethanolamine (PE), ether phospholipids (EtherPE), plasmalogens (Plasmalogen), phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidylinositol-ceramide (PI-Cer), cardiolipin (CL) and lysophospholipid lipid compositions of the above phospholipid lipids; wherein 10 wt% to 35 wt% of the lipids are glycolipid lipid compositions, wherein the glycolipid lipid compositions include, but are not limited to, ceramide (Cer), monogalactosyl diacylglycerol (MGDG), digalactosyl diacylglycerol (DGDG), sulforhamnosyl diacylglycerol (SQDG), glucuronic acid diacylglycerol (DGGA), and cholesterol ester (CE); Wherein 3 wt% to 20 wt% of the lipids are betaine lipid compositions, including, but not limited to, diacylglycerol trimethyl homoserine (DGTS), lyso-type diacylglycerol trimethyl homoserine (LDGTS; Wherein 3wt%~25wt% of lipids are neutral lipid lipid compositions, including, but not limited to, triglyceride lipid conjugates, diglyceride lipid compositions, monoglyceride lipid compositions, free fatty acids, but excluding the lipid compositions in the above-mentioned phospholipid lipid compositions, glycolipid lipid compositions and betaine ester lipid compositions.

[0011] Furthermore, 5 wt % to 80 wt % of EPA in the high-polarity algae oil P1 or the high-polarity algae oil P2 exists in the form of free fatty acids; The ratio of EPA to total omega-3 fatty acids is greater than 25%; Contains 5wt%~30wt% EPA.

[0012] Furthermore, the high-polarity algae oil P1 or the high-polarity algae oil P2 contains less than 8 wt % of arachidonic acid; More than 80 wt% of the chlorophyll was not degraded into pheophytin.

[0013] Furthermore, the paste-like crude algae oil contains 0.5-5 mg / g of ether phospholipids (EtherPE) and plasmalogens (Plasmalogen).

[0014] The present invention also provides the following solution: The invention discloses an application of high-polarity oil of Pseudomonas pseudochloris. High-polarity algae oil P1 or high-polarity algae oil P2 or paste-like crude algae oil is used for reducing human blood lipids, including triglycerides, total cholesterol and low-density lipoprotein.

[0015] The beneficial effects of the present invention are as follows: the present application obtains highly polar Nannochloropsis algae oil by extracting and purifying Nannochloropsis algae powder. Nannochloropsis algae fixes carbon dioxide through sunlight energy and is naturally rich in EPA. It can be obtained through artificial cultivation and is not affected by marine pollution and the gradual depletion of fisheries due to overfishing. It is also more friendly to consumers who are allergic to fish or shellfish. The polar lipids derived from Nannochloropsis algae have unique biomedical activities. For example, the polar lipids of Nannochloropsis algae can self-emulsify in the digestive tract, significantly improving the human body's absorption efficiency, and reduce the levels of triglycerides, cholesterol, and low-density lipoproteins released into the blood by inducing lipid droplet formation, reacylation, and inhibiting liver triglyceride synthesis. The polar lipids of Nannochloropsis algae can help fatty acids such as DHA to efficiently cross the blood-brain barrier. In the intestine, polar lipids are hydrolyzed by lipase into hemolytic polar lipids, which circulate through Lands in the liver. The polyunsaturated fatty acids are bound to the hemolytic polar lipids by the LPCAT3 enzyme. They are recognized by the Mfsd2a protein and enter the brain, delivering fatty acids such as DHA to the brain. Betaine esters of Microchloropsis spp. specifically bind to paraoxonase 1, thereby improving the function of high-density lipoprotein, reducing the oxidation of low-density lipoprotein, promoting cholesterol reflux through the ABCA1 pathway, inhibiting the transformation of macrophages into foam cells, and preventing and reducing the occurrence of atherosclerosis.

[0016] The above description is only an overview of the technical solution of the present invention. In order to more clearly understand the technical means of the present invention and implement it according to the contents of the specification, the following is a detailed description of the preferred embodiments of the present invention. DETAILED DESCRIPTION

[0017] The technical solution of the present invention will be described clearly and completely below. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0018] In the description of the present application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown, and are only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application. In addition, the terms "first", "second", "third" are only for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0019] In the description of the present application, it should be noted that unless otherwise explicitly specified and limited, the terms "mounting", "connecting", "connecting" should be understood in a broad sense, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the communication inside two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0020] In addition, the technical features involved in the different embodiments of the present application described below can be combined with each other as long as they do not conflict with each other.

[0021] A kind of proposed microcystis high polarity oil extraction method shown in a preferred embodiment of the present application, comprising the following steps: algal oil extraction, algal oil purification, S1, algal oil extraction: the contact of the microcystis powder and ethanol is heated to 50-70 degrees Celsius, then the filtrate phase is collected by centrifugation and filtration process, and the crude algal oil extract containing ethanol is obtained; S2, algal oil purification: the crude algal oil extract containing ethanol is placed in a cooling tank, and the crude algal oil extract containing ethanol is winterized by cooling; after winterization, the filter residue is separated by filtration, i.e. the high melting point fatty acid ester and other waxy impurities in the crude algal oil extract are removed; the filtrate phase is collected into a filtrate tank. The filtrate continues to evaporate, wash, and concentrate to obtain high polarity algal oil P1 with greatly reduced impurities.

[0022] The ethanol in the crude algal oil extract containing ethanol in S1 is evaporated to less than 2000 ppm, and the crude algal oil in paste form is obtained, wherein the proportion of polar lipids in total lipids is 45-70%, and the polar lipids include phospholipid lipid composition, glycolipid lipid composition and betaine lipid lipid composition; the content of total fatty acid in the crude algal oil is 30g-45g / 100g, and the content of eicosapentaenoic acid in the crude algal oil is 10g-17g / 100g.

[0023] The crude algal oil in paste form is heated to 55-65 degrees Celsius and contacted with ethanol; placing the mixture of the crude algae oil and ethanol in a cooling tank to winterize the crude algae oil mixture containing ethanol by lowering the temperature; After winterization is complete, the residue is separated by filtration, and the filtrate phase is collected and put into a filtrate tank. The filtrate is further evaporated, washed with water, and concentrated to obtain high-polarity algae oil P2.

[0024] High-polarity algae oil P1 or high-polarity algae oil P2 contains 1.5wt%~30wt% of eicosapentaenoic acid and 50wt%~90wt% of polar lipids, and column chromatography shows that the polar lipids are 56.47-82.30%; Polar lipids include phospholipid conjugates, glycolipid conjugates, betaine lipid conjugates, triglyceride conjugates, or diglyceride conjugates; Among them, 3wt%~20wt% of lipids are phospholipid conjugates, and column chromatography is 1.15-12.54%. Among them, 10wt%~35wt% of the lipids are glycolipid conjugates, and the column chromatography is 14.20-26.77%, Among them, 3wt%~15wt% of the lipids are betaine lipid conjugates, and the column chromatography is 5.77-11.91%, Among them, 3wt%~25wt% of the lipids are triglyceride conjugates or diglyceride conjugates, and the column chromatography is 6.68-16.81%.

[0025] The content of total fatty acids in high-polarity algae oil P1 or high-polarity algae oil P2 is 40-60g / 100g, the content of eicosapentaenoic acid in high-polarity algae oil is 15g-30g / 100g, and the proportion of polar lipids in total lipids is 50-90%.

[0026] The polar lipids of the high-polarity algae oil P1 or the high-polarity algae oil P2 include a phospholipid lipid composition, a glycolipid lipid composition, and a betaine lipid lipid composition; In the total lipids, 3w% to 25wt% of the lipids are phospholipid lipid compositions, wherein the phospholipid lipid compositions include, but are not limited to, phosphatidylcholine (PC), phosphatidylethanolamine (PE), ether phospholipids (EtherPE), plasmalogens (Plasmalogen), phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidylinositol-ceramide (PI-Cer), cardiolipin (CL) and lysophospholipid lipid compositions of the above phospholipid lipids; wherein 10 wt% to 35 wt% of the lipids are glycolipid lipid compositions, wherein the glycolipid lipid compositions include, but are not limited to, ceramide (Cer), monogalactosyl diacylglycerol (MGDG), digalactosyl diacylglycerol (DGDG), sulforhamnosyl diacylglycerol (SQDG), glucuronic acid diacylglycerol (DGGA), and cholesterol ester (CE); Wherein 3 wt% to 20 wt% of the lipids are betaine lipid compositions, including, but not limited to, diacylglycerol trimethyl homoserine (DGTS), lyso-type diacylglycerol trimethyl homoserine (LDGTS; Wherein 3wt%~25wt% of lipids are neutral lipid lipid compositions, including, but not limited to, triglyceride lipid conjugates, diglyceride lipid compositions, monoglyceride lipid compositions, free fatty acids, but excluding the lipid compositions in the above-mentioned phospholipid lipid compositions, glycolipid lipid compositions and betaine ester lipid compositions.

[0027] 5 wt% to 80 wt% of EPA in the high-polarity algal oil P1 or high-polarity algal oil P2 exists in the form of free fatty acids; The ratio of EPA to total omega-3 fatty acids is greater than 25%; Contains 5wt%~30wt% EPA.

[0028] The high-polarity algae oil P1 or the high-polarity algae oil P2 contains less than 8 wt % of arachidonic acid; More than 80 wt% of the chlorophyll was not degraded into pheophytin.

[0029] Paste-like crude algae oil contains 0.5~5mg / g of ether phospholipids (EtherPE) and plasmalogens (Plasmalogen).

[0030] The present invention adopts the "method for analyzing and detecting various polar components in algae oil" as the detection method. Various polar components in algae oil are analyzed and detected through the steps of material preparation, sample weighing, column filling preparation, elution of weak polar substances (neutral lipids), phospholipid elution, elution of other highly polar substances, concentration and weighing.

[0031] The present invention also provides the following solution: The invention discloses an application of high-polarity oil of Pseudomonas pseudochloris. High-polarity algae oil P1 or high-polarity algae oil P2 or paste-like crude algae oil is used for reducing human blood lipids, including triglycerides, total cholesterol and low-density lipoprotein.

[0032] Examples of lipid-lowering effects: Forty volunteers with dyslipidemia (aged 18 to 65 years, 20 men and 20 women) took soft capsules containing Nannochloropsis algae oil daily, with a daily dose of 1 to 2.5 grams, depending on individual weight and tolerance.

[0033] The study lasted 12 weeks (3 months), and fasting blood samples were collected from participants before and after the intervention to measure changes in blood lipid levels. Key indicators tested included triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C).

[0034]

[0035] In summary, the present invention provides a method for extracting highly polar oil from Pseudochlorophyllum and its application. This application obtains highly polar Pseudochlorophyllum oil by extracting and purifying Pseudochlorophyllum powder. Pseudochlorophyllum fixes carbon dioxide through sunlight energy and is naturally rich in EPA. It can be obtained through artificial cultivation and is not affected by marine pollution and the gradual depletion of fisheries due to overfishing. At the same time, it is more friendly to consumers who are allergic to fish or shellfish. The polar lipids derived from Pseudochlorophyllum have unique biomedical activities. For example, the polar lipids of Pseudochlorophyllum can self-emulsify in the digestive tract, significantly improving the human body's absorption efficiency, and reducing the levels of triglycerides, cholesterol, and low-density lipoproteins released into the blood by inducing lipid droplet formation, reacylation, and inhibiting liver triglyceride synthesis. Polar lipids from Nannochloropsis algae help fatty acids like DHA efficiently cross the blood-brain barrier. In the intestine, polar lipids are hydrolyzed by lipase into lytic polar lipids. These lipids circulate through the liver's Lands region, where the LPCAT3 enzyme binds polyunsaturated fatty acids to the lytic polar lipids. These lipids are then recognized by the Mfsd2a protein and enter the brain, delivering DHA and other fatty acids. Nannochloropsis algae's betaine esters specifically bind to paraoxonase 1, enhancing the function of high-density lipoproteins (HDLs), reducing the oxidation of low-density lipoproteins (LDLs), and promoting cholesterol regurgitation through the ABCA1 pathway. This inhibits the transformation of macrophages into foam cells, thereby preventing and reducing the occurrence of atherosclerosis.

[0036] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

Claims

1. A method for extracting highly polar oil from Nannochloropsis spp., characterized in that: The process includes the following steps: algae oil extraction, algae oil purification, S1. Algal oil extraction: Nannochloropsis algae powder is contacted with ethanol while heated to 50-70 degrees Celsius, and then the filtrate is collected by centrifugation and filtration to obtain a crude algal oil extract containing ethanol; S2. Algae Oil Purification: The crude ethanolic algae oil extract is placed in a cooling tank for winterization. After winterization, the residue is filtered to remove high-melting-point fatty acid esters and other waxy impurities from the crude algae oil extract. The filtrate is collected and transferred to a filtrate tank. Further evaporation, washing, and concentration of the filtrate yield highly polar algae oil P1 with significantly reduced impurities.

2. The ethanol in the ethanol-containing crude algae oil extract in S1 is evaporated to an ethanol content below 2000 ppm to obtain a paste-like crude algae oil, in which polar lipids account for 45-70% of the total lipids, and the polar lipids include a phospholipid lipid composition, a glycolipid lipid composition, and a betaine lipid lipid composition; the content of total fatty acids in the crude algae oil is 30g-45g / 100g, and the content of eicosapentaenoic acid in the crude algae oil is 10g-17g / 100g.

3. The method for extracting high-polarity oil from Nannochloropsis as claimed in claim 2, wherein: The crude algae oil paste is heated to 55-65 degrees Celsius and brought into contact with ethanol; placing the mixture of the crude algae oil and ethanol in a cooling tank to winterize the crude algae oil mixture containing ethanol by lowering the temperature; After winterization is complete, the residue is separated by filtration, and the filtrate phase is collected and put into a filtrate tank. The filtrate is further evaporated, washed with water, and concentrated to obtain high-polarity algae oil P2.

4. The method for extracting highly polar oil from Nannochloropsis spp. according to claim 1 or claim 3, wherein: High-polarity algae oil P1 or high-polarity algae oil P2 contains 1.5wt%~30wt% of eicosapentaenoic acid and 50wt%~90wt% of polar lipids, and column chromatography shows that the polar lipids are 56.47-82.30%; Polar lipids include phospholipid conjugates, glycolipid conjugates, betaine lipid conjugates, triglyceride conjugates, or diglyceride conjugates; Among them, 3wt%~20wt% of lipids are phospholipid conjugates, and column chromatography is 1.15-12.54%. Among them, 10wt%~35wt% of the lipids are glycolipid conjugates, and the column chromatography is 14.20-26.77%, Among them, 3wt%~15wt% of the lipids are betaine lipid conjugates, and the column chromatography is 5.77-11.91%, Among them, 3wt%~25wt% of the lipids are triglyceride conjugates or diglyceride conjugates, and the column chromatography is 6.68-16.81%.

5. The method for extracting highly polar oil from Nannochloropsis spp. according to claim 1 or claim 3, wherein: The content of total fatty acids in high-polarity algae oil P1 or high-polarity algae oil P2 is 40-60g / 100g, the content of eicosapentaenoic acid in high-polarity algae oil is 15g-30g / 100g, and the proportion of polar lipids in total lipids is 50-90%.

6. The method for extracting high-polarity oil from Nannochloropsis as claimed in claim 4, wherein: The polar lipids of the high-polarity algae oil P1 or the high-polarity algae oil P2 include a phospholipid lipid composition, a glycolipid lipid composition, and a betaine lipid lipid composition; In the total lipids, 3w% to 25wt% of the lipids are phospholipid lipid compositions, wherein the phospholipid lipid compositions include, but are not limited to, phosphatidylcholine, phosphatidylethanolamine, ether phospholipids, acetal phospholipids, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol-ceramide, cardiolipin, and lysophospholipid lipid compositions of the above phospholipid lipids; wherein 10 wt % to 35 wt % of the lipids are glycolipid lipid compositions, wherein the glycolipid lipid compositions include, but are not limited to, ceramides, monogalactosyl diacylglycerol, digalactosyl diacylglycerol, thioisorhamnosyl diacylglycerol, glucuronic acid diacylglycerol, and cholesterol esters; wherein 3 wt% to 20 wt% of the lipids are betaine lipid compositions, including, but not limited to, diacylglycerol trimethyl homoserine and lyso-type diacylglycerol trimethyl homoserine; Wherein 3wt%~25wt% of lipids are neutral lipid lipid compositions, including, but not limited to, triglyceride lipid conjugates, diglyceride lipid compositions, monoglyceride lipid compositions, free fatty acids, but excluding the lipid compositions in the above-mentioned phospholipid lipid compositions, glycolipid lipid compositions and betaine ester lipid compositions.

7. The method for extracting high-polarity oil from Nannochloropsis as claimed in claim 4, wherein: 5 wt% to 80 wt% of EPA in the high-polarity algal oil P1 or high-polarity algal oil P2 exists in the form of free fatty acids; The ratio of EPA to total omega-3 fatty acids is greater than 25%; Contains 5wt%~30wt% EPA.

8. The method for extracting high-polarity oil from Nannochloropsis as claimed in claim 4, wherein: The high-polarity algae oil P1 or the high-polarity algae oil P2 contains less than 8 wt % of arachidonic acid; More than 80 wt% of the chlorophyll was not degraded into pheophytin.

9. The method for extracting high-polarity oil from Nannochloropsis as claimed in claim 2, wherein: Paste-like crude algae oil contains 0.5~5mg / g of ether phospholipids and acetal phospholipids.

10. The use of the highly polar oil of Pseudomonas aeruginosa according to any one of claims 1 to 3, characterized in that: The high-polarity algae oil P1 or the high-polarity algae oil P2 or the crude algae oil in paste form is used to reduce blood lipids in the human body, including triglycerides, total cholesterol and low-density lipoprotein.

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