Culture equipment with shaking structure for cell culture

Abstract

The invention discloses culture equipment with a shaking structure for cell culture, and relates to the technical field of cell culture. The device comprises an annular shaking and vibrating disc, a hole body is formed in the end of the shaking and vibrating disc, a driving assembly is in spline clamping connection with the inner side face of the shaking and vibrating disc, the driving assembly is fixedly connected with the bottom of the inner side face of a box body, the surface of a shaking and vibrating ring is in a waveform shape, and the sides, close to each other, of the shaking and vibrating ring and the shaking and vibrating disc are matched. The buffering assembly is fixedly connected to the side, away from the shaking disc, of the shaking ring and is in rolling connection with the inner side face of the box body, the clamping assembly is fixedly connected with the inner side face of the hole body, and the wavy surface of the shaking disc is matched with the side, close to each other, of the shaking ring to drive the shaking disc to rotate and meanwhile to rise and fall in a reciprocating mode. The shaking ring is arranged on the culture bottle, more complex oscillation is carried out on the culture bottle, a better shaking effect is obtained, the buffer assembly supports and buffers the bottom of the shaking ring, the shaking ring is prevented from deforming, and the reciprocating lifting effect during shaking is maintained.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, in particular to a cell culture device with a shaking structure. Background Art

[0002] Shaking of cell culture is a key operation that uses specific equipment to evenly mix the cell suspension or culture medium in the culture flask, promote gas exchange, and prevent cell aggregation. The core is to use mechanical oscillation to drive the movement of the culture container, so that the liquid produces flow field effects such as convection and turbulence, ensuring full contact between cells and nutrients, while avoiding local hypoxia or accumulation of metabolic waste. The equipment is usually equipped with temperature control and gas regulation systems to simulate the in vivo environment, and the clamping components fix the culture flask through elastic strips, protective rings and other designs to prevent damage to cells during oscillation. During the shaking process, the oscillation frequency, amplitude, eccentricity and other parameters can be adjusted to adapt to the culture needs of adherent cells or suspended cells. It is widely used in biopharmaceuticals, stem cell research, vaccine production and other fields, and is crucial to maintaining cell activity and experimental repeatability.

[0003] The existing shaking structure is usually unable to quickly adjust the shaking effect when shaking the culture medium, which affects the culture effect of different cells. In view of this, how to design a shaking structure that can be easily adjusted is the technical problem to be solved by the present invention, so we propose a cell culture device with a shaking structure. Summary of the Invention

[0004] In order to solve the above technical problems, the present invention provides a cell culture device with a shaking structure, comprising:

[0005] A bottom plate, the top of which is fixedly connected to the box body;

[0006] A shaking mechanism, the shaking mechanism being arranged inside the box and fixedly connected to the inner side surface of the box;

[0007] Wherein, the shaking mechanism includes:

[0008] A vibration plate, wherein the vibration plate is arranged in an annular shape, and a hole is opened at one end of the vibration plate away from the bottom plate, and a plurality of holes are evenly distributed at the end of the vibration plate;

[0009] A drive assembly, wherein the drive assembly is spline-engaged with the inner side surface of the vibration plate, and the side of the drive assembly away from the vibration plate is fixedly connected to the bottom of the inner side surface of the box body;

[0010] A shaking ring, the shaking ring being arranged on a side of the shaking plate close to the bottom of the inner side of the box, and the surface of the shaking ring being arranged in a wave shape, the shaking ring being adapted to the side of the shaking plate close to each other, and the shaking ring being slidably connected to the side of the shaking plate close to each other;

[0011] A buffer assembly, wherein the buffer assembly is fixedly connected to a side of the vibration ring away from the vibration plate, and the side of the buffer assembly away from the vibration ring is rollingly connected to the inner side surface of the box;

[0012] A clamping assembly, the clamping assembly being disposed inside the hole body and fixedly connected to the inner side surface of the hole body;

[0013] The culture bottle is placed inside the hole body, and then the clamping assembly is driven to clamp the culture bottle. Culture bottles are placed inside several hole bodies, and then the driving assembly is started. The driving assembly drives the shaking plate to rotate, thereby shaking the culture bottle, and the shaking plate and the shaking ring produce relative rotation. Since the shaking ring is set to be waveform, and the shaking plate and the shaking ring are adapted to the side close to each other, when the shaking plate rotates, it produces reciprocating lifting, thereby performing more complex oscillation on the culture bottle and obtaining a better shaking effect. When the shaking plate and the shaking ring are squeezed, the shaking ring is subjected to vertical squeezing force, and the buffer assembly supports and buffers the bottom of the shaking ring to prevent the shaking ring from being deformed and maintain the reciprocating lifting effect during shaking.

[0014] Furthermore, the driving assembly includes a rotating telescopic rod, the surface of which is fixedly connected to a mounting plate, the mounting plate extends from a side of the rotating telescopic rod to a side of the vibration ring away from the vibration plate, and is fixedly connected to the inner side surface of the box body, a commutator is provided at one end of the rotating telescopic rod away from the mounting plate, the end of the rotating telescopic rod away from the mounting plate extends to the inside of the commutator, and the output end of the rotating telescopic rod is connected to the commutator transmission, and the rotating telescopic rod is started, the output end of the rotating telescopic rod drives the commutator transmission, and then the output end of the commutator rotates, and finally drives the vibration plate to rotate.

[0015] Furthermore, a mounting bracket is sleeved on the outer side of the output end of the commutator, and the output end surface of the commutator is slidably connected to the inner side surface of the mounting bracket, and the output end surface of the commutator is spline-engaged with the inner side surface of the mounting bracket, and the two ends of the mounting bracket are slidably connected to the inner side surface of the shaking plate, and the surface of the mounting bracket is spline-engaged with the inner side surface of the shaking plate. The output end of the commutator rotates, driving the mounting bracket to rotate, and then driving the engaged shaking plate to rotate. When the shaking plate is reciprocatingly lifted and lowered, the shaking plate and the mounting bracket slide, driving the rotating telescopic rod to retract and extend, driving the commutator to move radially along the shaking plate, and then the commutator slides inside the mounting bracket, so that the rotation center of the commutator output end is offset from the middle of the mounting bracket, thereby generating eccentric rotation. The eccentric rotation causes the liquid inside the culture bottle to spiral, and cooperate with the reciprocating lifting to obtain a better shaking effect. At the same time, the adjustable distance between the rotation center of the commutator output end and the middle of the mounting bracket can adjust the eccentric shaking effect to adapt to different cell cultures.

[0016] Furthermore, the inner side surface of the mounting frame is symmetrically provided with eccentric dovetail grooves, and the inner side surfaces of the two eccentric dovetail grooves are slidably connected with eccentric dovetail blocks, and the sides of the two eccentric dovetail blocks close to each other are fixedly connected to the output end surface of the commutator. The output end of the commutator drives the eccentric dovetail blocks to rotate, and drives the mounting frame connected to it through the eccentric dovetail groove to rotate, while not affecting the radial sliding of the eccentric dovetail blocks along the vibration plate inside the eccentric dovetail groove.

[0017] Furthermore, the inner side surface of the vibrating plate is symmetrically provided with lifting dovetail grooves, and the inner sides of the two lifting dovetail grooves are slidably connected with lifting dovetail blocks, and the lifting dovetail blocks are arranged in the middle of the lifting dovetail grooves, and the sides of the two lifting dovetail blocks close to each other are fixedly connected to the surface of the mounting frame. The mounting frame rotates, driving the lifting dovetail blocks to rotate, and driving the vibrating plate connected to it through the lifting dovetail grooves to rotate, and the lifting dovetail blocks are arranged in the middle of the lifting dovetail grooves, which will not affect the reciprocating lifting of the vibrating plate.

[0018] Furthermore, a sliding seat is provided at one end of the commutator close to the bottom of the inner side surface of the box body, the inner side surface of the sliding seat is slidably connected to the commutator surface, and the side of the sliding seat away from the commutator is fixedly connected to the bottom of the inner side surface of the box body. The commutator slides radially along the vibration plate, and the commutator slides inside the sliding seat, supporting the bottom of the commutator without affecting the radial movement of the commutator, thereby avoiding affecting the eccentric rotation of the vibration plate.

[0019] Furthermore, the buffer assembly includes a buffer rod, which is fixedly connected to the side of the vibration ring away from the vibration plate. Several buffer rods are arranged along the circumference of the vibration ring, and the buffer rods are arranged at the trough on the side of the vibration ring away from the vibration plate. The outer sleeve of the buffer rod is provided with a buffer sleeve, and the inner side surface of the buffer sleeve is slidingly connected to the surface of the buffer rod. The side of the buffer sleeve away from the buffer rod is rollingly connected with a ball, and the surface of the ball is rollingly connected to the bottom of the inner side surface of the box. The buffer rod supports the bottom trough of the vibration ring and supports it when the top peak of the vibration ring is squeezed, thereby avoiding deformation of the vibration ring and ensuring the reciprocating lifting effect. When the side of the top peak of the vibration ring is squeezed, the buffer rod and the buffer sleeve are offset, and the ball rolls. The movable support of the ball can avoid breakage at the connection between the buffer rod and the vibration ring.

[0020] Furthermore, a spring is provided inside the buffer sleeve, and the spring is fixedly connected to the bottom of the inner side surface of the buffer sleeve. The other end of the spring is fixedly connected to one end of the buffer rod located inside the buffer sleeve. The buffer rod slides inside the buffer sleeve, and the spring is deformed. The elastic resistance of the spring supports the bottom of the vibration ring and at the same time buffers the impact of the vibration plate on the vibration ring.

[0021] Further, the clamping assembly comprises two clamping plates symmetrically arranged inside the hole body, and the clamping plates are arranged in a ring shape, the two clamping plates are symmetrically provided with driving rods on the side close to each other, the driving rods are fixedly connected with the surface of the clamping plates, the two clamping plates are symmetrically provided with fixing rings at the interval, and the fixing rings are arranged on the side close to each other of the two driving rods, the inner side surface of the fixing ring is fixedly connected with the surface of the two driving rods, and the side away from each other of the two fixing rings is fixedly connected with the inner side surface of the hole body; the driving rod is started, the driving rod is extended or retracted, the clamping plate is driven to move, the two clamping plates are close to each other, and the culture bottle is clamped; and the two clamping plates with adjustable spacing can clamp culture bottles of different sizes.

[0022] Further, the inside of the clamping plate is inlaid with a rubber plate, the surface of the rubber plate is fixedly connected with the inner side surface of the clamping plate, the two clamping plates are fixedly connected with rubber pads on the side close to each other, the rubber pads are arranged in a wave shape, and a plurality of rubber pads are uniformly distributed on the surface of the clamping plate; the rubber pads can increase the friction between the clamping plate and the culture bottle, so that better clamping effect is obtained; the wave-shaped rubber pad can increase the vertical friction resistance of the culture bottle, so that better clamping effect is further obtained; the clamping plate deforms during clamping, driving the rubber plate to deform; the rubber plate can buffer the clamping plate, and the elastic force of the rubber plate after restoring the deformation can avoid fatigue of the clamping plate and ensure the clamping effect during continuous use.

[0023] The present application has the following beneficial effects:

[0024] 1. The present application is provided with a shaking mechanism, the shaking disc and the shaking ring are relatively rotated, the shaking ring is arranged in a wave shape, and the side close to each other of the shaking disc and the shaking ring is matched, the shaking disc rotates while reciprocating up and down, so that more complex oscillation is generated on the culture bottle, better shaking effect is obtained, and when the shaking disc and the shaking ring are extruded, the shaking ring is vertically extruded, the buffer assembly supports and buffers the bottom of the shaking ring, deformation of the shaking ring is avoided, and the reciprocating up and down effect during shaking is maintained.

[0025] 2. The present application is provided with a driving assembly, the reversing gear moves along the radial direction of the shaking disc, then the reversing gear slides inside the mounting frame, so that the rotation center of the output end of the reversing gear is offset from the middle part of the mounting frame, eccentric rotation is generated, the liquid in the culture bottle is spiraled due to the eccentric rotation, better shaking effect is obtained by cooperating with the reciprocating up and down, and the interval between the rotation center of the output end of the adjustable reversing gear and the middle part of the mounting frame can adjust the eccentric shaking effect, so that different cell cultures can be adapted.

[0026] 3. The present invention sets a buffer assembly, and the buffer rod supports the bottom trough of the shaking ring and supports the top peak of the shaking ring when it is squeezed, thereby avoiding deformation of the shaking ring and ensuring the reciprocating lifting effect. When the side of the top peak of the shaking ring is squeezed, the buffer rod and the buffer sleeve are offset, and the ball rolls. The movable support of the ball can avoid breakage at the connection between the buffer rod and the shaking ring. The elastic resistance of the spring supports the bottom of the shaking ring and buffers the impact of the shaking plate on the shaking ring.

[0027] 4. The present invention provides a clamping assembly, and two clamping plates with adjustable spacing can clamp culture bottles of different sizes. The rubber pad can increase the friction between the clamping plate and the culture bottle, thereby obtaining a better clamping effect, and the corrugated rubber pad can increase the vertical friction resistance with the culture bottle, further obtaining a better clamping effect. During clamping, the clamping plate deforms, driving the rubber plate to deform. The rubber plate can cushion the clamping plate, and the elastic force of the rubber plate to restore the deformation can avoid fatigue of the clamping plate and ensure the clamping effect during continuous use. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a schematic structural diagram of a cell culture device with a shaking structure according to the present invention;

[0029] Figure 2 A schematic diagram of the internal structure of the cell culture device with a shaking structure according to the present invention;

[0030] Figure 3 This is a schematic structural diagram of the shaking mechanism of the present invention;

[0031] Figure 4 This is a schematic diagram of the assembly structure of the vibration plate of the present invention;

[0032] Figure 5 This is a schematic diagram of the structure of the drive assembly of the present invention;

[0033] Figure 6 This is a schematic diagram of the cross-sectional structure of the vibration plate of the present invention;

[0034] Figure 7 This is a schematic structural diagram of the buffer assembly of the present invention;

[0035] Figure 8 This is a schematic diagram of the cross-sectional structure of the buffer sleeve of the present invention;

[0036] Figure 9 This is a schematic diagram of the cross-sectional structure of the vibration plate of the present invention;

[0037] Figure 10 It is a schematic diagram of the structure of the clamping assembly of the present invention.

[0038] In the figure: 1, bottom plate; 2, box body; 3, shaking mechanism; 31, shaking disc; 32, hole body; 33, driving assembly; 331, rotating telescopic rod; 332, mounting plate; 333, commutator; 334, mounting frame; 335, lifting dovetail groove; 336, lifting dovetail block; 337, eccentric dovetail groove; 338, eccentric dovetail block; 339, sliding seat; 34, shaking ring; 35, buffer assembly; 351, buffer rod; 352, buffer sleeve; 353, ball; 354, spring; 36, clamping assembly; 361, clamping plate; 362, driving rod; 363, fixed ring; 364, rubber pad; 365, rubber plate. DETAILED DESCRIPTION

[0039] The application will be further described below in conjunction with the drawings and specific embodiments. The embodiments of the application are given for illustrative and descriptive purposes only, and are not exhaustive or limiting of the application. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments are chosen and described in order to best explain the principles of the application and its practical application, and to enable others skilled in the art to understand the application for various embodiments with various modifications as are suited to the particular use contemplated.

[0040] Embodiment 1, please refer to Figures 1-6 The application is a culture device for cell culture with a shaking structure, comprising:

[0041] The bottom plate 1 is fixedly connected at the top with the box body 2;

[0042] The shaking mechanism 3 is arranged inside the box body 2, and the shaking mechanism 3 is fixedly connected with the inner side surface of the box body 2;

[0043] The shaking mechanism 3 comprises:

[0044] The shaking disc 31 is arranged in a ring shape, and the end of the shaking disc 31 away from the bottom plate 1 is provided with a hole body 32, and the hole body 32 is uniformly distributed at the end of the shaking disc 31;

[0045] The driving assembly 33 is splined with the inner side surface of the shaking disc 31, and the side of the driving assembly 33 away from the shaking disc 31 is fixedly connected with the inner side surface bottom of the box body 2;

[0046] The shaking ring 34 is arranged at the side of the shaking disc 31 close to the inner side surface bottom of the box body 2, and the surface of the shaking ring 34 is arranged in a wave shape, the side of the shaking ring 34 close to the shaking disc 31 is adapted, and the side of the shaking ring 34 close to the shaking disc 31 is slidingly connected;

[0047] The buffering assembly 35 is fixedly connected to the side of the shaking ring 34 away from the shaking disc 31. The side of the buffering assembly 35 away from the shaking ring 34 is rollingly connected to the inner side of the box body 2.

[0048] The clamping assembly 36 is arranged inside the hole body 32 and is fixedly connected to the inner side of the hole body 32.

[0049] The culture bottles are placed inside the hole bodies 32. Then the clamping assembly 36 is driven to clamp the culture bottles. The hole bodies 32 are placed inside the hole bodies 32. Then the driving assembly 33 is started to drive the shaking disc 31 to rotate, so that the culture bottles are shaken. The shaking disc 31 and the shaking ring 34 are relatively rotated. Since the shaking ring 34 is arranged in a wave shape and the side of the shaking disc 31 and the shaking ring 34 close to each other is matched, the shaking disc 31 is reciprocally lifted while rotating, so that the culture bottles are more complexly shaken, and a better shaking effect is obtained. When the shaking disc 31 and the shaking ring 34 are extruded, the shaking ring 34 is vertically extruded. The buffering assembly 35 supports and buffers the bottom of the shaking ring 34, so that the shaking ring 34 is prevented from being deformed and the reciprocally lifting effect during shaking is maintained.

[0050] The driving assembly 33 comprises a rotary telescopic rod 331. The surface of the rotary telescopic rod 331 is fixedly connected with a mounting plate 332. The side of the mounting plate 332 away from the rotary telescopic rod 331 extends to the side of the shaking ring 34 away from the shaking disc 31 and is fixedly connected to the inner side of the box body 2. The end of the rotary telescopic rod 331 away from the mounting plate 332 is provided with a reverser 333. The end of the rotary telescopic rod 331 away from the mounting plate 332 extends to the inside of the reverser 333. The output end of the rotary telescopic rod 331 is drivingly connected with the reverser 333. The rotary telescopic rod 331 is started. The output end of the rotary telescopic rod 331 drives the reverser 333 to drive. Then the output end of the reverser 333 is rotated to finally drive the shaking disc 31 to rotate.

[0051] The outer side of the output end of the commutator 333 is provided with a mounting bracket 334. The output end surface of the commutator 333 is slidably connected to the inner side surface of the mounting bracket 334, and the output end surface of the commutator 333 is spline-engaged with the inner side surface of the mounting bracket 334. The two ends of the mounting bracket 334 are slidably connected to the inner side surface of the shaking plate 31, and the surface of the mounting bracket 334 is spline-engaged with the inner side surface of the shaking plate 31. The output end of the commutator 333 rotates, driving the mounting bracket 334 to rotate, and then driving the connected shaking plate 31 Rotation. When the shaking plate 31 is reciprocatingly lifted and lowered, the shaking plate 31 and the mounting frame 334 slide, driving the rotating telescopic rod 331 to extend and retract, driving the commutator 333 to move radially along the shaking plate 31, and then the commutator 333 slides inside the mounting frame 334, so that the rotation center of the output end of the commutator 333 is offset from the middle of the mounting frame 334, thereby generating eccentric rotation. The eccentric rotation causes the liquid inside the culture bottle to spiral, and cooperate with the reciprocating lifting to obtain a better shaking effect.

[0052] The inner side surface of the mounting frame 334 is symmetrically provided with eccentric dovetail grooves 337, and the inner sides of the two eccentric dovetail grooves 337 are slidably connected with eccentric dovetail blocks 338. The sides of the two eccentric dovetail blocks 338 close to each other are fixedly connected to the output end surface of the commutator 333. The output end of the commutator 333 drives the eccentric dovetail blocks 338 to rotate, and drives the mounting frame 334 clamped with it through the eccentric dovetail grooves 337 to rotate, while not affecting the radial sliding of the eccentric dovetail blocks 338 inside the eccentric dovetail grooves 337 along the vibration plate 31.

[0053] The inner side surface of the vibration plate 31 is symmetrically provided with lifting dovetail grooves 335, and the inner sides of the two lifting dovetail grooves 335 are slidably connected with lifting dovetail blocks 336, and the lifting dovetail blocks 336 are set in the middle of the lifting dovetail grooves 335. The sides of the two lifting dovetail blocks 336 close to each other are fixedly connected to the surface of the mounting frame 334. The mounting frame 334 rotates, driving the lifting dovetail blocks 336 to rotate, and driving the vibration plate 31 connected to it through the lifting dovetail grooves 335 to rotate, and the lifting dovetail blocks 336 are set in the middle of the lifting dovetail grooves 335, which will not affect the reciprocating lifting of the vibration plate 31.

[0054] A sliding seat 339 is sleeved on one end of the commutator 333 close to the bottom of the inner side surface of the box body 2. The inner side surface of the sliding seat 339 is slidably connected to the surface of the commutator 333. The side of the sliding seat 339 away from the commutator 333 is fixedly connected to the bottom of the inner side surface of the box body 2. The commutator 333 slides radially along the vibration plate 31. The commutator 333 slides inside the sliding seat 339, supporting the bottom of the commutator 333 without affecting the radial movement of the commutator 333, thereby avoiding affecting the eccentric rotation of the vibration plate 31.

[0055] Example 2, please refer to Figures 1-10The buffer assembly 35 includes a buffer rod 351, which is fixedly connected to the side of the vibration ring 34 away from the vibration plate 31. A plurality of buffer rods 351 are arranged along the circumference of the vibration ring 34, and the buffer rods 351 are arranged at the trough of the vibration ring 34 away from the vibration plate 31. The outer sleeve of the buffer rod 351 is provided with a buffer sleeve 352, and the inner side surface of the buffer sleeve 352 is slidably connected to the surface of the buffer rod 351. The side of the buffer sleeve 352 away from the buffer rod 351 is rollingly connected with a ball 353, and The surface of the ball 353 is rollingly connected to the bottom of the inner side surface of the box body 2, and the buffer rod 351 supports the bottom trough of the vibration ring 34, and supports it when the top peak of the vibration ring 34 is squeezed, thereby avoiding deformation of the vibration ring 34 and ensuring the reciprocating lifting effect. When the side of the top peak of the vibration ring 34 is squeezed, the buffer rod 351 and the buffer sleeve 352 are offset, and the ball 353 rolls. The movable support of the ball 353 can avoid breakage at the connection between the buffer rod 351 and the vibration ring 34.

[0056] A spring 354 is provided inside the buffer sleeve 352. The spring 354 is fixedly connected to the bottom of the inner side surface of the buffer sleeve 352. The other end of the spring 354 is fixedly connected to one end of the buffer rod 351 located inside the buffer sleeve 352. The buffer rod 351 slides inside the buffer sleeve 352, and the spring 354 is deformed. The elastic resistance of the spring 354 supports the bottom of the vibration ring 34, and at the same time buffers the impact of the vibration plate 31 on the vibration ring 34.

[0057] The clamping assembly 36 includes a clamping plate 361, which is symmetrically arranged with two clamping plates 361 inside the hole body 32, and the clamping plates 361 are arranged in a ring shape. The driving rods 362 are symmetrically arranged on the sides of the two clamping plates 361 that are close to each other. The driving rods 362 are fixedly connected to the surfaces of the clamping plates 361, and a fixing ring 363 is symmetrically arranged at the interval between the two clamping plates 361, and the fixing ring 363 is arranged on the sides where the two driving rods 362 are close to each other. The inner side surface of the fixing ring 363 is fixedly connected to the surface of the connection between the two driving rods 362, and the sides where the two fixing rings 363 are away from each other are fixedly connected to the inner side surface of the hole body 32. When the driving rod 362 is started, the driving rod 362 is extended and retracted, driving the clamping plates 361 to move, and the two clamping plates 361 are close to each other to clamp the culture bottle, and the two clamping plates 361 with adjustable spacing can clamp culture bottles of different sizes.

[0058] The inner part of the clamping plate 361 is inlaid with a rubber plate 365, the surface of the rubber plate 365 is fixedly connected with the inner side of the clamping plate 361, the two clamping plates 361 are fixedly connected with rubber pads 364 on the side close to each other, the rubber pads 364 are arranged in a wave shape, and a plurality of rubber pads 364 are uniformly distributed on the surface of the clamping plate 361, the rubber pads 364 can increase the friction between the clamping plate 361 and the culture bottle, so that a better clamping effect is obtained, and the wave-shaped rubber pads 364 can increase the vertical friction resistance of the culture bottle, so that a better clamping effect is further obtained. When clamping, the clamping plate 361 deforms, driving the rubber plate 365 to deform, the rubber plate 365 can buffer the clamping plate 361, and the elastic force of the rubber plate 365 restoring the deformation can avoid fatigue of the clamping plate 361, and ensure the clamping effect during continuous use.

[0059] In use, the culture bottle is placed inside the hole body 32, the drive rod 362 is started, the drive rod 362 is telescopic, driving the clamping plate 361 to move, the two clamping plates 361 are close to each other, clamping the culture bottle, the rotary telescopic rod 331 is started, the output end of the rotary telescopic rod 331 drives the commutator 333 to drive, then the output end of the commutator 333 rotates, the output end of the commutator 333 drives the eccentric dovetail block 338 to rotate, driving the mounting frame 334 connected with the eccentric dovetail groove 337 to rotate, driving the lifting dovetail block 336 to rotate, driving the shaking disc 31 connected with the lifting dovetail groove 335 to rotate, the shaking disc 31 and the shaking ring 34 rotate relative to each other, because the shaking ring 34 is arranged in a wave shape, and the side close to each other between the shaking disc 31 and the shaking ring 34 is matched, when the shaking disc 31 rotates, it reciprocates, when the shaking disc 31 reciprocates, the shaking disc 31 and the mounting frame 334 slide, driving the rotary telescopic rod 331 to extend and retract, driving the commutator 333 to move along the radial direction of the shaking disc 31, then the commutator 333 slides in the mounting frame 334, so that the rotation center of the output end of the commutator 333 is offset from the middle of the mounting frame 334, so as to produce eccentric rotation, the top wave peak of the shaking ring 34 is extruded, the buffer rod 351 slides in the buffer sleeve 352, and the spring 354 deforms. The elastic resistance of the spring 354 supports the bottom of the shaking ring 34.

[0060] Obviously, the embodiments described are only a part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art and related fields without creative labor shall belong to the scope of protection of the present application. The structures, devices and operation methods not specifically described and explained in the present application are implemented according to the conventional means in the art, unless otherwise specified and limited.

Claims

1. A cell culture device having a shaking structure, characterized in that: include: A bottom plate (1), the top of which is fixedly connected to a box body (2); A shaking mechanism (3), the shaking mechanism (3) being arranged inside the box (2), and the shaking mechanism (3) being fixedly connected to the inner side surface of the box (2); Wherein, the shaking mechanism (3) comprises: A shaking plate (31), the shaking plate (31) is arranged in a ring shape, and a hole (32) is opened at one end of the shaking plate (31) away from the bottom plate (1), and a plurality of holes (32) are evenly distributed at the end of the shaking plate (31); A drive assembly (33), wherein the drive assembly (33) is spline-engaged with the inner side surface of the vibration plate (31), and a side of the drive assembly (33) away from the vibration plate (31) is fixedly connected to the bottom of the inner side surface of the box body (2); A shaking ring (34), the shaking ring (34) being arranged on a side of the shaking plate (31) close to the bottom of the inner side of the box body (2), and the surface of the shaking ring (34) being arranged in a wave shape, the shaking ring (34) being adapted to the side of the shaking plate (31) close to each other, and the shaking ring (34) being slidably connected to the side of the shaking plate (31) close to each other; A buffer assembly (35), wherein the buffer assembly (35) is fixedly connected to a side of the vibration ring (34) away from the vibration plate (31), and the side of the buffer assembly (35) away from the vibration ring (34) is rollingly connected to the inner side surface of the box body (2); A clamping assembly (36) is provided inside the hole body (32), and the clamping assembly (36) is fixedly connected to the inner side surface of the hole body (32).

2. The cell culture device with a shaking structure according to claim 1, characterized in that: The driving assembly (33) comprises a rotating telescopic rod (331), a surface of the rotating telescopic rod (331) being fixedly connected to a mounting plate (332), a side of the mounting plate (332) away from the rotating telescopic rod (331) extending to a side of the vibration ring (34) away from the vibration plate (31), and being fixedly connected to the inner side surface of the box (2), a commutator (333) being provided at one end of the rotating telescopic rod (331) away from the mounting plate (332), an end of the rotating telescopic rod (331) away from the mounting plate (332) extending to the interior of the commutator (333), and an output end of the rotating telescopic rod (331) being transmission-connected to the commutator (333).

3. The cell culture device with a shaking structure according to claim 2, characterized in that: A mounting bracket (334) is sleeved on the outer side of the output end of the commutator (333); the output end surface of the commutator (333) is slidably connected to the inner side surface of the mounting bracket (334); the output end surface of the commutator (333) is spline-engaged with the inner side surface of the mounting bracket (334); both ends of the mounting bracket (334) are slidably connected to the inner side surface of the vibration plate (31); and the surface of the mounting bracket (334) is spline-engaged with the inner side surface of the vibration plate (31).

4. The cell culture device with a shaking structure according to claim 3, characterized in that: The inner side surface of the mounting frame (334) is symmetrically provided with eccentric dovetail grooves (337), the inner sides of the two eccentric dovetail grooves (337) are slidably connected with eccentric dovetail blocks (338), and the sides of the two eccentric dovetail blocks (338) that are close to each other are fixedly connected to the output end surface of the commutator (333).

5. The cell culture device with a shaking structure according to claim 4, characterized in that: The inner side surface of the shaking plate (31) is symmetrically provided with lifting dovetail grooves (335), and the inner sides of the two lifting dovetail grooves (335) are slidably connected with lifting dovetail blocks (336), and the lifting dovetail blocks (336) are arranged in the middle of the lifting dovetail grooves (335), and the sides of the two lifting dovetail blocks (336) close to each other are fixedly connected to the surface of the mounting frame (334).

6. The cell culture device with a shaking structure according to claim 5, characterized in that: A sliding seat (339) is sleeved on one end of the commutator (333) close to the bottom of the inner side surface of the box body (2); the inner side surface of the sliding seat (339) is slidably connected to the surface of the commutator (333); and the side of the sliding seat (339) away from the commutator (333) is fixedly connected to the bottom of the inner side surface of the box body (2).

7. The cell culture device with a shaking structure according to claim 6, characterized in that: The buffer assembly (35) includes a buffer rod (351), the buffer rod (351) being fixedly connected to a side of the vibration ring (34) away from the vibration plate (31), a plurality of buffer rods (351) being arranged along the circumference of the vibration ring (34), and the buffer rods (351) being arranged at a trough on a side of the vibration ring (34) away from the vibration plate (31), a buffer sleeve (352) being provided on the outside of the buffer rod (351), and an inner side surface of the buffer sleeve (352) being slidably connected to a surface of the buffer rod (351), a ball (353) being rollingly connected to a side of the buffer sleeve (352) away from the buffer rod (351), and a surface of the ball (353) being rollingly connected to the bottom of the inner side surface of the box body (2).

8. The cell culture device with a shaking structure according to claim 7, characterized in that: A spring (354) is provided inside the buffer sleeve (352), and the spring (354) is fixedly connected to the bottom of the inner side of the buffer sleeve (352). The other end of the spring (354) is fixedly connected to one end of the buffer rod (351) located inside the buffer sleeve (352).

9. The cell culture device with a shaking structure according to claim 8, characterized in that: The clamping assembly (36) includes a clamping plate (361), two of which are symmetrically arranged inside the hole body (32), and the clamping plates (361) are arranged in a ring shape. A driving rod (362) is symmetrically arranged on the side where the two clamping plates (361) are close to each other, and the driving rod (362) is fixedly connected to the surface of the clamping plate (361). A fixing ring (363) is symmetrically arranged at the interval between the two clamping plates (361), and the fixing ring (363) is arranged on the side where the two driving rods (362) are close to each other. The inner side surface of the fixing ring (363) is fixedly connected to the surface of the connection between the two driving rods (362), and the side where the two fixing rings (363) are away from each other is fixedly connected to the inner side surface of the hole body (32).

10. The cell culture device with a shaking structure according to claim 9, characterized in that: A rubber plate (365) is embedded inside the clamping plate (361), and the surface of the rubber plate (365) is fixedly connected to the inner side of the clamping plate (361). The two clamping plates (361) are fixedly connected to the sides close to each other with rubber pads (364). The rubber pads (364) are arranged in a corrugated shape, and a plurality of rubber pads (364) are evenly distributed on the surface of the clamping plate (361).

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