Application of trans-cinnamic acid in preparation of product for resisting skin photoaging

Abstract

The invention belongs to the technical field of microorganisms, and finds that trans-cinnamic acid is a key metabolite of microorganism staphylococcus epidermidis H62-3 for the first time, so that a new method is provided for preparation of the trans-cinnamic acid, and meanwhile, the problem of environmental pollution caused by chemical synthesis of the trans-cinnamic acid is also avoided. In addition, the invention also relates to an application of trans-cinnamic acid in preparation of a product for resisting skin photoaging. The trans-cinnamic acid provided by the invention has the effect of resisting skin photoaging, can effectively relieve the transcriptional level of senescence-related genes p53, p21 and p16 and inflammatory factors IL-1beta, IL-6 and TNF-alpha induced by UVB irradiation, reduces the level of reactive oxygen species (ROS), inhibits the expression of matrix metalloproteinases MMP9, MMP3 and MMP1, promotes the synthesis of I-type collagen (COL1), and can be used for preparing anti-aging drugs for treating skin aging. And the activity of beta-galactosidase (SA-beta-gal) related to aging is reduced.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of microorganisms, and particularly relates to application of trans-cinnamic acid in preparation of a product for resisting skin photoaging. BACKGROUND

[0002] With the growth of age and the influence of external environment, the body will experience a series of physiological and structural changes, eventually leading to the appearance of aging. In this process, the aging of the skin is particularly significant, which mainly manifests in weakening of the skin barrier function, degradation of collagen and elastic fibers, and increase of pigmentation. The skin aging is mainly divided into two types of natural aging and photoaging, wherein the photoaging is the main form of facial aging, accounting for more than 80%, and the photoaging is a skin aging process caused by long-term ultraviolet (UVA and UVB) radiation. These radiations can penetrate the epidermis of the skin, induce the production of free radicals, and then damage the DNA, proteins and lipids of cells, leading to cell dysfunction or even death.

[0003] The skin, as the first line of defense of the human body, not only provides a physical barrier, but also participates in complex physiological and biochemical processes. In these processes, the skin microbiota plays a crucial role, especially bacteria, which interact with host cells by secreting various bioactive metabolites and signaling molecules, and then regulate the dynamic balance of skin physiological functions. Studies have shown that human skin aging is accompanied by changes in the skin microbiota. Among them, Staphylococcus epidermidis, as a skin resident symbiotic bacteria, has many influences on human health. It not only can promote the maturation of the immune system, but also can secrete antibacterial substances to inhibit harmful bacteria, so as to maintain the balance of the skin microbiota. In addition, the strain can help the host synthesize ceramide by secreting sphingomyelinase, and strengthen the skin barrier. These functions show that Staphylococcus epidermidis may play a key role in skin health and aging. Research on the application of the metabolites of Staphylococcus epidermidis H62-3 in skin health and aging has important significance for the utilization and development of microbial resources and new functional skin care ingredients. SUMMARY

[0004] In view of the deficiencies of the prior art, the application aims to provide application of trans-cinnamic acid in preparation of a product for resisting skin photoaging, wherein the trans-cinnamic acid is a key metabolite of Staphylococcus epidermidis H62-3.

[0005] Trans-cinnamic acid (tCA) is a kind of phenylpropanoid compound with important biological activity. At present, trans-cinnamic acid is mainly synthesized by chemical method, but this method has problems such as complex process, harsh reaction conditions (high temperature and high pressure), and environmental pollution. Microbial fermentation as a green and sustainable production method has significant advantages. Therefore, it is of great significance to develop microbial resources that can not only produce trans-cinnamic acid efficiently but also have direct anti-photoaging function.

[0006] To achieve the purpose of the application, the following technical solutions are adopted in the application: In a first aspect, the application provides a preparation method of trans-cinnamic acid with anti-skin photoaging effect, the preparation method comprising isolating and purifying the metabolic product of Staphylococcus epidermidis H62-3.

[0007] The isolation, purification and screening of the above Staphylococcus epidermidis H62-3: the collected Staphylococcus epidermidis H62-3 is diluted with 0.05 mL of glycerol preservation solution and 0.15 mL of sterile water, and the above diluent is spread on a TSA solid plate and placed in a 37℃ constant temperature incubator for inverted culture for 24 hours. According to the colony characteristics of Staphylococcus epidermidis, single colonies are picked and four-zone separation is performed, and placed in a 37℃ constant temperature incubator for inverted culture for 24 hours. 16S rRNA sequencing is performed to determine that the single colony of Staphylococcus epidermidis is preserved at -80℃. The whole genome RNA of different strains of Staphylococcus epidermidis is extracted, reverse transcribed into cDNA, and RT-qPCR experiment is performed to screen the Staphylococcus epidermidis strain H62-3 with high expression of genes closely related to moisturizing efficacy.

[0008] In some embodiments of the application, the preparation method of the metabolic product of Staphylococcus epidermidis H62-3 containing trans-cinnamic acid comprises the following steps: (1) The activated Staphylococcus epidermidis strain H62-3 is inoculated with single colonies in TSB liquid medium, and placed in a 32-38℃ shaker for 14-18 hours of shaking culture as seed liquid; (2) The seed liquid is inoculated in TSB liquid medium with a volume fraction of 3%-5% inoculation amount, and placed in a 32-38℃ shaker for 14-18 hours of shaking culture to obtain a fermentation broth; (3) The fermentation broth is centrifuged, and the bacterial body precipitate is discarded; the fermentation broth supernatant is concentrated by 5 times volume through rotary evaporation, which is the metabolic product of Staphylococcus epidermidis strain H62-3 containing trans-cinnamic acid.

[0009] In some embodiments of the application, the method for detecting trans-cinnamic acid by isolating and purifying the metabolic product of Staphylococcus epidermidis H62-3 is liquid chromatography (LC-MS method).

[0010] In a second aspect, the present application provides a metabolite of Staphylococcus epidermidis H62-3 having an anti-skin photoaging effect, wherein the metabolite of Staphylococcus epidermidis H62-3 comprises a key metabolite trans-cinnamic acid.

[0011] In some embodiments of the present application, the method for preparing the metabolite of Staphylococcus epidermidis H62-3 is the same as the first aspect described above.

[0012] In a third aspect, the present application provides the use of trans-cinnamic acid, which is a metabolite of Staphylococcus epidermidis H62-3, or the metabolite of Staphylococcus epidermidis H62-3 as described in the second aspect above, in the preparation of a product for anti-skin photoaging, wherein the trans-cinnamic acid is prepared by the method as described in the first aspect above.

[0013] In some embodiments of the present application, the trans-cinnamic acid down-regulates the transcription level of UVB irradiation-induced senescence-related genes (p53, p21 and p16).

[0014] In some embodiments of the present application, the trans-cinnamic acid inhibits the transcription level of UVB irradiation-induced inflammatory factors (IL-1β, IL-6 and TNF-α).

[0015] In some embodiments of the present application, the trans-cinnamic acid reduces the level of UVB irradiation-induced reactive oxygen species (ROS).

[0016] In some embodiments of the present application, the trans-cinnamic acid inhibits the expression of UVB irradiation-induced matrix metalloproteinases (MMP9, MMP3 and MMP1) and promotes the synthesis of type I collagen (COL1).

[0017] In some embodiments of the present application, the trans-cinnamic acid reduces the activity of UVB irradiation-induced senescence-associated β-galactosidase (SA-β-gal).

[0018] In some embodiments of the present application, the product is a toner, emulsion, serum, cream, paste, mask or lyophilized powder.

[0019] In some embodiments of the present application, the concentration of the trans-cinnamic acid in the product is 10-100 μM.

[0020] Further, the concentration of the trans-cinnamic acid in the product is 10 μM, 50 μM or 100 μM.

[0021] The Staphylococcus epidermidis used in the present application is Staphylococcus epidermidis) H62-3, deposited in the China General Microbiological Culture Collection Center (CGMCC) on February 21, 2023, with the accession number CGMCC No. 26640. Staphylococcus epidermidis Staphylococcus epidermidis ) H62-3 is described in the granted Chinese invention patent CN116286543B with the filing date of April 14, 2023.

[0022] The Staphylococcus epidermidis H62-3 derived from the face of a healthy person is identified by whole genome of bacteria and ANI, SNP, InDel analysis, and it is found that the Staphylococcus epidermidis H62-3 has numerous base mutations, additions or deletions compared with the Staphylococcus epidermidis model strain CGMCC 1.4260, thereby causing changes in proteins and further affecting its biological functions.

[0023] The beneficial effects of the present application are: (1) The present application first discovers that trans-cinnamic acid is a key metabolic product of the microorganism Staphylococcus epidermidis H62-3, which provides a new method for the preparation of trans-cinnamic acid, and also avoids the environmental pollution problem existing in the chemical synthesis of trans-cinnamic acid.

[0024] (2) The trans-cinnamic acid or the metabolic product of the Staphylococcus epidermidis H62-3 containing trans-cinnamic acid provided by the present application has an anti-skin photoaging effect, can effectively alleviate the transcription level of aging-related genes (p53, p21, p16) and inflammatory factors (IL-1β, IL-6, TNF-α) induced by UVB irradiation, reduce the level of reactive oxygen species (ROS), inhibit the expression of matrix metalloproteinases (MMP9, MMP3, MMP1) and promote the synthesis of type I collagen (COL1), and reduce the activity of aging-related β-galactosidase (SA-β-gal). BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 Differential metabolic product analysis of Staphylococcus epidermidis H62-3 and 1.4260; Figure 2 LC-MS analysis of the fermentation metabolic product of Staphylococcus epidermidis H62-3; Figure 3 Effect of trans-cinnamic acid on mRNA expression of aging-related genes (p53, p21, p16) of HaCaT photoaged cells; Figure 4 Effect of trans-cinnamic acid on mRNA expression of inflammatory factors (IL-1β, IL-6, TNF-α) of HaCaT photoaged cells; Figure 5 Effect of trans-cinnamic acid on ROS fluorescence intensity in HaCaT photoaged cells; Figure 6 The effect of trans-cinnamic acid on the expression of matrix metalloproteinases (MMP9, MMP3, and MMP1) and type I collagen (COL1) in HFF photoaged cells; Figure 7 This is the effect of trans-cinnamic acid on SA-β-gal positive cells in a full-thickness skin photoaging model. DETAILED DESCRIPTION

[0026] The present invention will be further described below with reference to specific examples. It should be understood that the specific examples described herein are intended only to explain the present invention and are not intended to limit the scope of the invention. Where specific techniques or conditions are not specified in the examples, the techniques or conditions described in the literature in the art or in accordance with the product instructions are used. Where the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased through regular channels.

[0027] The present invention provides a strain of Staphylococcus epidermidis ( Staphylococcus epidermidis )H62-3, deposited in the General Microbiology Center of China Culture Collection Administration of Microorganisms (CGMCC), with the deposit number CGMCC No. 26640 and the deposit date of February 21, 2023.

[0028] Tryptone soy broth (TSB) medium: tryptone 17.0 g / L, soy peptone 3.0 g / L, sodium chloride 5.0 g / L, potassium dihydrogen phosphate 2.5 g / L, glucose 2.5 g / L, autoclave at 121°C for 15 min and set aside.

[0029] Tryptone soy broth solid medium (TSA): tryptone 17.0 g / L, soy peptone 3.0 g / L, sodium chloride 5.0 g / L, potassium hydrogen phosphate 2.5 g / L, glucose 2.5 g / L, agar powder 15 g / L, autoclave at 121°C for 15 min, pour into plates, and set aside.

[0030] The present invention discloses a Gram-positive Staphylococcus epidermidis strain H62-3. Its colonies on TSA plates are small, white, and smooth, opaque, rounded projections. Microscopically, the colonies are spherical, devoid of flagella, incapable of motility, and non-spore-forming. Its optimal growth temperature is 32-38°C. It grows rapidly in TSB liquid medium, entering the logarithmic phase in approximately two hours and the stationary phase in approximately 12 hours.

[0031] Example 1: Preparation of Staphylococcus epidermidis H62-3 fermentation metabolites 1. Isolation, purification and screening of Staphylococcus epidermidis H62-3 (1) Sample collection Recruit volunteers of different age groups (18-90 years old) with healthy facial skin, without acne, pustules, inflammation, erythema, etc., without oral antibiotic preparations within the past 2 months and without external antibiotic preparations within the past 2 weeks, and without cleaning / washing the face within 12 hours of sampling.

[0032] The sampler wears a mask and disposable sterile gloves. Sterile physiological saline is used to soak a sterile sampling cotton swab. The cotton swab is 2 cm long, and a 4 cm long mark is made on the sampling site. The cotton swab is wiped back and forth 50 times, taking about 30 seconds, and the area is about 8 cm 2 . After wiping, the cotton swab is placed in a glycerol preservation tube, the lid is tightly sealed, and the number is recorded.

[0033] (2) Skin flora culture, isolation, purification, and preservation 0.05 mL of glycerol preservation solution is added to 0.15 mL of sterile water for dilution. The above dilution is spread on a TSA solid plate, and placed in a 37℃ constant temperature incubator for inverted culture for 24 hours. According to the colony characteristics of Staphylococcus epidermidis, single colonies are picked and separated by four-zone line, and placed in a 37℃ constant temperature incubator for inverted culture for 24 hours. 16S rRNA sequencing is performed to determine the single colony of Staphylococcus epidermidis preserved at -80℃.

[0034] (3) Functional bacteria screening The bacterial whole genome RNA of different strains of Staphylococcus epidermidis is extracted, reverse transcribed into cDNA, and subjected to RT-qPCR experiment to screen Staphylococcus epidermidis strain H62-3 with highly expressed genes closely related to moisturizing efficacy.

[0035] 2. The preparation method of the metabolic product of Staphylococcus epidermidis H62-3 comprises the following steps: (1) The single colony of the purified and activated Staphylococcus epidermidis strain H62-3 is inoculated in TSB liquid medium, and placed in a 32℃ shaker for 14 hours of shaking culture as seed liquid; (2) The seed liquid is inoculated in TSB liquid medium at a volume fraction of 3%, and placed in a 32℃ shaker for 14 hours of shaking culture to obtain a fermentation broth; (3) The fermentation broth is centrifuged, and the bacterial body precipitate is discarded. The fermentation broth supernatant is concentrated by rotary evaporation by 5 times the volume to obtain the fermentation metabolic product of Staphylococcus epidermidis H62-3.

[0036] Example 2: Identification of trans-cinnamic acid The H62-3 fermentation metabolites prepared in Example 1 were subjected to non-targeted metabolomics detection by gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS). The detection steps mainly included sample pretreatment, metabolite extraction, LC-MS and GC-MS full scan detection, data preprocessing and statistical analysis, etc. The chromatographic conditions of GC-MS were as follows: DB-5MS capillary column (30 m x 0.25 mm x 0.25 μm, Agilent J&W Scientific, Folsom, CA, USA), carrier gas was high-purity helium (purity not less than 99.999%), flow rate was 1.0 mL / min, temperature of the injection port was 260 °C. The injection volume was 1 μL, unsplit injection, solvent delay was 5 min. The programmed temperature was as follows: the initial temperature of the column oven was 60 °C, maintained for 0.5 min; programmed to 125 °C at 8 °C / min; programmed to 210 °C at 8 °C / min; programmed to 270 °C at 15 °C / min; programmed to 305 °C at 20 °C / min, maintained for 5 min. The chromatographic conditions of LC-MS were as follows: chromatographic column: ACQUITY UPLC HSS T3 (100 mm x 2.1 mm, 1.8 μm); column temperature: 45 °C; mobile phase: A-water (containing 0.1% formic acid), B-acetonitrile; flow rate: 0.35 mL / min; injection volume: 2 μL. Based on non-targeted metabolomics, combined with metabolomics data processing software, qualitative and relative quantitative analysis of the raw data were performed, and the raw data were standardized and pretreated. The data analysis included multivariate statistical analysis (PCA, PLS-DA, OPLS-DA) and univariate statistical analysis (T test, fold difference), and the differential metabolites were screened according to the criteria of P<0.05, VIP>1, and |log2FC|>2, and the relative contents of the differential metabolites were sorted, in which the relative content of trans-cinnamic acid was the highest, as shown in P Figure 2. Figure 1

[0037] ​The fermentation metabolites of Staphylococcus epidermidis H62-3 prepared in Example 1 were detected by LC-MS method in positive ion mode for targeted metabolomics detection, 100 μL sample was added to 700 μL methanol, vortexed for 60 seconds, 17000 g, centrifuged for 15 minutes, and the supernatant was taken for standby; standard solution was prepared with 75% methanol, and the standard solution and internal standard were mixed at 1:1; the chromatographic column was ACQUITY UPLC HSS T3 (50 mm×2.1 mm, 1.8 μm); the column temperature was 40°C; the mobile phase was A-0.25% formic acid aqueous solution+2 mM ammonium formate, B-acetonitrile; the flow rate was 0.40 mL / min; the injection volume was 5 μL; the AB6600 mass spectrometer was based on MS1 mode for primary mass spectrum data acquisition, and the positive mode was collected, and the ESI ion source parameters were set as follows: ion source drying gas temperature (Gas Temp): 500°C, curtain gas (Curtain Gas): 25 Psi, collision gas (Collision Gas): 10 Psi, ion spray voltage (IonSpray Voltage): 5500V, atomization temperature (Temperature): 500°C; the MultiQuant3.0.3 analysis software was used to make standard curve by internal standard method with standard response and corresponding concentration.

[0038] LC-MS analysis showed that there was still a single chromatographic peak at the same retention time position as the control trans-cinnamic acid, so it was determined that trans-cinnamic acid was contained in the fermentation metabolites of Staphylococcus epidermidis H62-3. Thus it is confirmed that Staphylococcus epidermidis H62-3 is a trans-cinnamic acid producing strain, as shown in Figure 2

[0039] Example 3: Effect of trans-cinnamic acid on mRNA expression of HaCaT photoaging cell senescence-related genes (p53, p21, p16) Keratinocytes HaCaT were inoculated into 6-well plates at a density of 2.5 × 10 5 cells / mL, 2 mL system, and incubated for 12 hours. The cells were treated with 10 μM, 50 μM and 100 μM of trans-cinnamic acid for 24 hours. When the cell density was 60-80%, UVB irradiation (60 mJ / cm 2 ) was performed, and the RNA of each group of cells was extracted 24 hours after irradiation, and then reverse transcribed into cDNA, and then RT-qPCR was performed to detect the expression of senescence-related genes (p53, p21, p16).

[0040] The results showed that compared with the control group, the mRNA level of HaCaT cell senescence-related genes (p53, p21, p16) was significantly increased after UVB irradiation​P <0.05, while the expression levels of senescence-related genes in HaCaT cells treated with different concentrations of trans-cinnamic acid were reduced ( P <0.05), such as Figure 3 shown.

[0041] Example 4: Effect of trans-cinnamic acid on the mRNA expression of inflammatory factors (IL-1β, IL-6, TNF-α) in HaCaT photoaged cells Keratinocytes HaCaT were cultured at a density of 2.5 × 10 5 HaCaT cells were seeded into 6-well plates at a density of 10 cells / mL in a 2 mL system and incubated for 12 hours until the cells were fully attached. HaCaT cells were treated with 10 μM, 50 μM, and 100 μM trans-cinnamic acid for 24 hours. When the cell density was 60-80%, UVB irradiation (60 mJ / cm 2 After 24 hours of irradiation, the cells were cultured and the RNA of each group was extracted and reverse transcribed into cDNA, and then RT-qPCR was performed to detect the expression of inflammatory factors (IL-1β, IL-6, and TNF-α).

[0042] The results showed that compared with the control group, the expression of inflammatory factors (IL-1β, IL-6, TNF-α) mRNA levels in HaCaT cells were significantly increased after UVB irradiation ( P <0.05, while the expression levels of inflammatory factors in HaCaT cells treated with different concentrations of trans-cinnamic acid were reduced ( P <0.05), such as Figure 4 shown.

[0043] Example 5: Effect of trans-cinnamic acid on ROS fluorescence intensity in HaCaT photoaged cells Keratinocytes HaCaT were cultured at a density of 2.5 × 10 5 HaCaT cells were seeded into 6-well plates at a density of 10 cells / mL in a 2 mL system and incubated for 12 hours until the cells were fully attached. HaCaT cells were treated with 10 μM, 50 μM, and 100 μM trans-cinnamic acid for 24 hours. When the cell density was 60-80%, UVB irradiation (60 mJ / cm 2 ), and reactive oxygen species (ROS) staining was performed after culturing the cells for 24 h after irradiation.

[0044] The cell culture medium was first aspirated, and the cells were washed twice with PBS. 1 mL of Hochest33342 diluted with serum-free medium (1:100, final concentration 1x) was added to each well, and the cells were incubated at 37°C for 10 minutes. The dye-containing culture solution was aspirated, and the cells were washed three times with PBS. DCFH-DA diluted with serum-free medium (1:1000, final concentration 10 μmol / L) was added to each well, and the cells were incubated at 37°C for 30 minutes. The dye-containing culture solution was aspirated, and the cells were washed three times with PBS, and then observed under a fluorescence microscope.

[0045] The results show that different concentrations of trans-cinnamic acid can reduce the fluorescence intensity of reactive oxygen species (ROS) in the UVB-induced HaCaT cell photoaging model to varying degrees, as shown in Figure 5 .

[0046] Example 6: Effect of trans-cinnamic acid on the expression of matrix metalloproteinases (MMP9, MMP3 and MMP1) and type I collagen (COL1) in HFF photoaging cells Fibroblasts HFF were seeded in a 6-well plate at a density of 1.0 × 10 5 cells / mL, 2 mL system, and incubated for 12 hours until the cells were fully adherent. The HFF were treated with 10 μM, 50 μM and 100 μM trans-cinnamic acid for 24 hours. When the cell density was 60-80%, UVB irradiation (100 mJ / cm 2 ) was performed, and the total protein of the cells was collected 24 hours after irradiation. The expression levels of matrix metalloproteinases (MMP9, MMP3, MMP1) and type I collagen (COL1) were detected by Western blot.

[0047] The results show that, compared with the control group, the protein expression levels of MMP9, MMP3 and MMP1 in HFF cells were significantly up-regulated after UVB irradiation ( P <0.05), and the synthesis of COL1 was inhibited ( P <0.05), while different concentrations of trans-cinnamic acid treatment can alleviate the high expression of MMPs and low expression of COL1 induced by UVB ( P <0.05), as shown in Figure 6 .

[0048] Example 7: Effect of trans-cinnamic acid on SA-β-gal positive cells in a full-thickness skin photoaging model In this example, T-Skin TMThe three-dimensional in vitro reconstructed full-thickness skin model was used to construct a full-thickness skin photoaging model. First, the full-thickness skin model was pretreated with 10 μM, 50 μM and 100 μM trans-cinnamic acid for 24 hours; then the skin model was subjected to UVB irradiation for 3 consecutive days, with a dose of 180 mJ / cm 2 for each irradiation; after irradiation, the sample was incubated for 24 hours, and then collected for frozen section.

[0049] After the frozen section was warmed at room temperature for 30 minutes, it was washed with PBS for 3 times, blocked with immunofluorescence blocking solution for 2 hours, incubated with the first antibody (1:1000) at room temperature for 60 minutes, then incubated with the second antibody (1:500) for 60 minutes, and finally mounted with DAPI-containing anti-fluorescence quencher. After the above steps, fluorescence microscopy was performed for photographing.

[0050] The results showed that after UVB irradiation, the epidermal layer of the skin model was significantly stained with senescence-associated β-galactosidase (SA-β-gal) positive cells, and the proportion of SA-β-gal positive cells in the full-thickness skin photoaging model induced by UVB was reduced to different degrees after treatment with 10 μM, 50 μM and 100 μM trans-cinnamic acid, as shown in Figure 7 .

[0051] Although the embodiments of the present application have been shown and described above, it should be understood that the above embodiments are exemplary and should not be construed as limiting the present application, and those skilled in the art can make changes, modifications, replacements and variations to the above embodiments within the scope of the present application.

Claims

1. A method for preparing trans-cinnamic acid having an anti-skin photoaging effect, characterized in that: The preparation method comprises separating and purifying the metabolites of Staphylococcus epidermidis H62-3.

2. A metabolite of Staphylococcus epidermidis H62-3 with anti-skin photoaging effect, characterized in that: The metabolites of Staphylococcus epidermidis H62-3 contain trans-cinnamic acid, a key metabolite.

3. Use of trans-cinnamic acid or the metabolite of Staphylococcus epidermidis H62-3 according to claim 2 in the preparation of a product for resisting skin photoaging, characterized in that: The trans-cinnamic acid is a metabolite of Staphylococcus epidermidis H62-3, and the trans-cinnamic acid is prepared by the preparation method according to claim 1.

4. The use according to claim 3, characterized in that The trans-cinnamic acid downregulates the transcription level of aging-related genes induced by UVB irradiation, and the aging-related genes downregulated by UVB irradiation are p53, p21 and p16. The trans-cinnamic acid inhibits the transcription level of inflammatory factors induced by UVB irradiation, and the inflammatory factors inhibited by UVB irradiation are IL-1β, IL-6 and TNF-α.

5. The use according to claim 3, characterized in that The trans-cinnamic acid reduces the level of reactive oxygen species induced by UVB irradiation.

6. The use according to claim 3, characterized in that The trans-cinnamic acid inhibits the expression of matrix metalloproteinases induced by UVB irradiation and promotes the synthesis of type I collagen (COL1). The matrix metalloproteinases induced by UVB irradiation are MMP9, MMP3 and MMP1.

7. The use according to claim 3, characterized in that The trans-cinnamic acid reduces the activity of senescence-related β-galactosidase induced by UVB irradiation.

8. The use according to claim 3, characterized in that The product is toner, lotion, essence, cream, ointment, facial mask or freeze-dried powder.

9. The use according to claim 3, characterized in that The concentration of trans-cinnamic acid in the product is 10-100 μM.

10. The use according to claim 9, characterized in that The concentration of trans-cinnamic acid in the product is 10 μM, 50 μM or 100 μM.

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