Molecular marker rapid detection device for livestock breeding molecular breeding

Abstract

The invention provides a molecular marker rapid detection device for livestock breeding molecular breeding, and relates to the technical field of molecular marker detection.The molecular marker rapid detection device comprises a detection box, a reagent adding structure and a cleaning structure are arranged on the upper side of the detection box, a detection structure, a stirring structure and a rotating structure are arranged in the detection box, and the stirring structure corresponds to the cleaning structure; a transmission structure is arranged at the bottom in the detection box, a driving structure is arranged at the bottom of the detection box, the driving structure corresponds to the transmission structure, the transmission structure corresponds to the stirring structure, a mounting structure is arranged in the rotating structure, a breeding structure is arranged in the mounting structure, and a connecting structure is arranged between the mounting structure and the breeding structure. The color change can be observed through the detection structure for rapid detection, and stirring is performed through the stirring structure, so that the reagent mixing efficiency is improved, the reagent reaction time is shortened, and the working efficiency is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of molecular marker detection, in particular to a molecular marker rapid detection device for animal husbandry molecular breeding. Background Art

[0002] Molecular marker breeding utilizes the characteristics of molecular markers being closely linked to genes that determine target traits. By detecting molecular markers, the presence of target genes can be detected, thereby achieving the purpose of selecting target traits. It is a new breeding method with the advantages of being fast, accurate, and not affected by environmental conditions. Commonly used methods for detecting molecular markers mostly use detection probes to observe the linkage effect between molecular markers and target genes to achieve the detection of molecular markers. In the existing molecular breeding marker detection process, it is usually necessary to add reagents, stir, centrifuge, etc. to the solution reagents. However, the existing structures for adding reagents, stirring, centrifuging, etc. are usually dispersed, resulting in the need to send the breeding test tubes to different devices for operation during the cultivation process, resulting in low work efficiency and high cost.

[0003] To this end, the present invention provides a molecular marker rapid detection device for animal husbandry molecular breeding. Summary of the Invention

[0004] In view of the deficiencies in the prior art, the present invention aims to provide a rapid detection device for molecular markers for molecular breeding in animal husbandry to solve the problems raised in the above-mentioned background technology. The present invention can perform rapid detection by observing color changes through a detection structure and stirring through a stirring structure, thereby accelerating the efficiency of reagent mixing, thereby reducing the reaction time of the reagents and improving work efficiency; it can realize the stirring, centrifugation, and detection processes of the reagents without the need to use multiple operating devices, reducing the cost of the device and greatly improving the work efficiency of the device, and can perform different operating steps on different breeding test tubes, thereby improving the flexibility of the device; the stirring structure can be cleaned by a cleaning structure to prevent reagent residues on the stirring structure, thereby preventing the next stirring from being affected, preventing errors in the results, and improving the accuracy of the device; it can prevent external microorganisms from contaminating the inside of the breeding test tube, thereby preventing errors in the detection results and improving the success rate of breeding.

[0005] In order to achieve the above object, the present application is realized by the following technical scheme: a livestock breeding molecular breeding molecular marker rapid detection device, including detection box, the detection box upper side is equipped with reagent adding structure and cleaning structure, detection box is equipped with detection structure, stirring structure and rotating structure, the stirring structure corresponds with the cleaning structure, the bottom of the detection box is equipped with transmission structure, the bottom of the detection box is equipped with drive structure, the drive structure corresponds with transmission structure, transmission structure corresponds with stirring structure, rotating structure is equipped with mounting structure inside, mounting structure is equipped with breeding structure inside, mounting structure and breeding structure are equipped with connecting structure, connecting structure corresponds with stirring structure and transmission structure, the top of breeding structure is equipped with sealing structure, sealing structure corresponds with reagent adding structure and stirring structure.

[0006] Further, the reagent adding structure includes a first reagent tank and a second reagent tank fixed on the detection box, the first reagent tank and the second reagent tank are the same structure, the top of the first reagent tank and the second reagent tank is provided with a feeding port, the feeding port is connected with a plug, the bottom of the first reagent tank and the second reagent tank is fixed with an adding pipe, the adding pipe is located in the detection box, and the adding pipe is equipped with a first electric valve.

[0007] Further, the detection structure includes a fixed rod fixed in the detection box, a plurality of cameras are fixed on the side of the fixed rod, a display screen is fixed on the upper side of the detection box, the display screen corresponds with the camera, the rotating structure includes a rotating frame arranged in the detection box, and the rotating frame is fixed with a transmission cylinder.

[0008] Further, the drive structure includes an electric cylinder and a motor fixed on the lower side of the detection box, the output end of the electric cylinder is fixed with a rotating head, the rotating head is rotatably connected with the rotating frame, the transmission structure includes a first rotating shaft and a plurality of second rotating shafts rotatably arranged in the bottom of the detection box, and the first rotating shaft is fixedly connected with the output end of the motor.

[0009] Further, the first rotating shaft is fixed with a first gear, the second rotating shaft is fixed with a second gear, the transmission cylinder is provided with a third gear and a fourth gear, the third gear is fixedly connected with the transmission cylinder, the fourth gear is rotatably connected with the transmission cylinder, a plurality of positioning rods are fixed in the bottom of the detection box, a plurality of positioning grooves are formed in the lower side of the third gear, and the positioning grooves correspond with the positioning rods.

[0010] Further, the first rotating shaft and the second rotating shaft are fixed with a first plug on the upper side, the mounting structure includes a plurality of mounting outer cylinders arranged on the rotating frame, the rotating frame is provided with a fixed groove, the mounting outer cylinders are fixed in the fixed groove, the mounting outer cylinders are fixed with mounting inner cylinders, and the breeding structure includes a breeding test tube corresponding to the mounting inner cylinder.

[0011] Furthermore, a slot is provided on the circumference of the breeding test tube, and a block is installed in the inner cylinder for rotation. The block corresponds to the slot, and both the block and the slot are annular structures. The block is made of elastic material.

[0012] Furthermore, the outer mounting tube and the rotating frame are both provided with through slots, which correspond to the first rotating axis and the second rotating axis. The connecting structure includes a first rotating block rotatably engaged with the inner mounting tube, a first slot is provided in the first rotating block, the first slot corresponds to the first plug-in block, a second plug-in block is fixed on the first rotating block, the bottom of the breeding test tube is rotatably engaged with the second rotating block, a second slot is provided in the second rotating block, and the second slot corresponds to the second plug-in block.

[0013] Furthermore, a piston is fixed on the second rotating block, a groove is opened in the second rotating block, a first slide groove is opened on both sides of the groove, an insertion rod is slidably fitted in the first slide groove, a plurality of third slots are opened at the bottom of the breeding test tube, the third slots correspond to the insertion rod, a push block is slidably fitted in the groove, a first spring is fixed between the push block and the groove wall of the groove, the push block is located between the two insertion rods, the bottom of the push block is a conical structure, the stirring structure includes a plurality of telescopic rods fixed in the detection box, the telescopic rod includes a first rod body and a second rod body, a second slide groove is opened in the first rod body, the second rod body slides in the second slide groove, a plurality of second springs are fixed between the second rod body and the groove wall of the second slide groove, the end of the second rod body is rotatably fitted with an electromagnet, and the electromagnet corresponds to the push block.

[0014] Furthermore, the cleaning structure includes multiple water tanks fixed on the detection box, a water pump is fixed in the water tank, a water channel is opened in the second rod body, the water outlet of the water pump is connected with the water channel, the water inlet of the water pump is connected with the water tank, a water outlet is opened on one side of the water channel, a second electric valve is installed in the water outlet, the sealing structure includes a sealing cover fixed on the breeding test tube, a push plate is installed on the sealing cover, a sealing block is installed in the sealing cover, an addition port is opened in the sealing block, a blocking plate is rotatably fitted in the addition port, a torsion spring is installed between the addition port and the blocking plate, a push rod is fixed to the lower side of the push plate, and multiple third springs are fixed between the push plate and the sealing cover.

[0015] Beneficial effects of the present invention:

[0016] 1. A detection structure, a stirring structure and a rotating structure are installed in the detection box. The detection structure can be used to observe color changes for rapid detection, and the stirring structure can be used for stirring, thereby accelerating the efficiency of reagent mixing, thereby reducing the reagent reaction time and improving work efficiency. The rotating structure can also be used to drive the breeding test tube to rotate, thereby adjusting the position of the breeding test tube, and changing the operation process of the breeding test tube, thereby realizing different operations on the breeding test tube.

[0017] 2. A transmission structure is installed at the bottom of the detection box. A drive structure is installed at the bottom of the detection box. In combination with a stirring structure, a reagent adding structure, a cleaning structure and a connecting structure, the stirring, centrifugation and detection processes of the reagents can be realized. There is no need to use multiple operating devices, which reduces the cost of the device and greatly improves the working efficiency of the device. Different operating steps can be performed on different breeding test tubes, thereby improving the flexibility of the device and expanding the scope of application of the device. Multiple operating steps can be performed simultaneously, thereby greatly improving the working efficiency of the device.

[0018] 3. The stirring structure can be cleaned through the cleaning structure to prevent reagent residues on the stirring structure, thereby preventing the impact on the next stirring, preventing errors in the results, and improving the accuracy of the device. When a large number of reagents need to be added, the cleaning structure can be used to assist in adding reagents, thereby ensuring that the device has a better detection effect.

[0019] 4. A sealing structure is installed on the top of the breeding structure, which can be used to seal the breeding test tube, thereby preventing the reagent from spilling during the centrifugation process, ensuring the success of the centrifugation, and preventing external microorganisms from contaminating the inside of the breeding test tube, thereby preventing errors in the test results and improving the success rate of breeding. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic diagram of the overall assembly three-dimensional structure of a rapid detection device for molecular markers used in livestock molecular breeding according to the present invention;

[0021] Figure 2 This is a schematic diagram of the overall assembly cross-sectional structure of a rapid molecular marker detection device for livestock molecular breeding according to the present invention;

[0022] Figure 3 This is a schematic diagram of the assembly cross-sectional structure of the detection box and the water tank in a rapid detection device for molecular markers used in livestock molecular breeding according to the present invention;

[0023] Figure 4 for Figure 3 Schematic diagram at A in the middle;

[0024] Figure 5 for Figure 3 Schematic diagram at B in the middle;

[0025] Figure 6 for Figure 3 Schematic diagram at C in the middle;

[0026] Figure 7 This is a schematic diagram of the assembled three-dimensional structure of the transmission structure in a rapid detection device for molecular markers used in animal husbandry molecular breeding according to the present invention;

[0027] Figure 8 It is an exploded view of a transmission structure in a livestock breeding molecular breeding molecular marker rapid detection device;

[0028] Figure 9 It is an assembly perspective structural diagram of a sealing cover in the livestock breeding molecular breeding molecular marker rapid detection device;

[0029] Figure 10 It is an assembly perspective structural diagram of a first rotating shaft and a first gear in the livestock breeding molecular breeding molecular marker rapid detection device;

[0030] Figure 11 It is an assembly structural diagram of a breeding test tube structure in the livestock breeding molecular breeding molecular marker rapid detection device;

[0031] In the figure: 1, detection box; 2, first reagent box; 3, second reagent box; 4, water tank; 6, water pump; 7, adding pipe; 8, first electric valve; 9, fixed rod; 10, camera; 11, display screen; 12, rotating frame; 13, electric cylinder; 14, rotating head; 15, transmission cylinder; 16, first gear; 17, second gear; 18, third gear; 19, fourth gear; 20, first rotating shaft; 21, second rotating shaft; 22, positioning rod; 23, positioning groove; 24, first plug; 25, mounting outer cylinder; 26, mounting inner cylinder; 27, through groove; 28, fixed groove; 29, first insertion slot; 30, first rotating block; 31, second plug; 32, breeding test tube; 33, second rotating block; 34, second insertion slot; 35, groove; 36, insertion rod; 37, third insertion slot; 38, first spring; 39, first sliding groove; 40, push block; 41, piston; 42, telescopic rod; 43, first rod body; 44, second rod body; 45, second sliding groove; 46, second spring; 47, water channel; 48, water outlet; 49, second electric valve; 50, electromagnet; 51, stirring rod; 52, feed inlet; 53, block; 54, push rod; 55, motor; 56, clamping groove; 57, clamping block; 58, sealing cover; 59, adding port; 60, third spring; 61, blocking plate; 62, push plate; 63, sealing block. DETAILED DESCRIPTION

[0032] In order to make the technical means, creative features, purposes and effects realized by the present application easy to understand, the present application will be further described below in combination with specific embodiments.

[0033] Please refer to Figures 1 to 11The present invention provides a technical solution: a rapid detection device for molecular markers for molecular breeding in animal husbandry, comprising a detection box 1, wherein a reagent adding structure and a cleaning structure are installed on the upper side of the detection box 1, a detection structure, a stirring structure and a rotating structure are installed inside the detection box 1, and the stirring structure corresponds to the cleaning structure; a transmission structure is installed at the bottom of the detection box 1, a driving structure is installed at the bottom of the detection box 1, the driving structure corresponds to the transmission structure, the transmission structure corresponds to the stirring structure, an installation structure is installed in the rotating structure, a breeding structure is installed in the installation structure, a connecting structure is installed between the installation structure and the breeding structure, the connecting structure corresponds to the stirring structure and the transmission structure, a sealing structure is installed on the top of the breeding structure, and the sealing structure corresponds to the reagent adding structure and the stirring structure.

[0034] In this embodiment, the reagent adding structure includes a first reagent box 2 and a second reagent box 3 fixed on the detection box 1. The first reagent box 2 and the second reagent box 3 have the same structure. The tops of the first reagent box 2 and the second reagent box 3 are both provided with feed ports 52, and a blocking block 53 is fixed in the feed port 52. The bottoms of the first reagent box 2 and the second reagent box 3 are both fixed with an adding tube 7, the adding tube 7 is located in the detection box 1, and a first electric valve 8 is installed in the adding tube 7.

[0035] Specifically, by opening the block 53, reagents can be added to the first reagent box 2 and the second reagent box 3 through the feed port 52. When reagents need to be added to the breeding test tube 32, the first electric valve 8 is started to open, so that the reagents in the first reagent box 2 or the second reagent box 3 flow out from the adding tube 7 and enter. Reagents can be added for color development of molecular marker detection, thereby realizing rapid detection of molecular markers. The detection method is relatively simple and intuitive, and can achieve better detection effects.

[0036] The detection structure includes a fixed rod 9 fixed in the detection box 1, and multiple cameras 10 are fixed on the circumference of the fixed rod 9. A display screen 11 is fixed on the upper side of the detection box 1, and the display screen 11 corresponds to the camera 10. The rotating structure includes a rotating frame 12 installed in the detection box 1, and a transmission cylinder 15 is fixed on the lower side of the rotating frame 12.

[0037] Specifically, multiple cameras 10 can be used to monitor the reagents in different breeding test tubes 32. The breeding test tubes 32 are made of transparent materials, so that the color changes in the breeding test tubes 32 can be observed more intuitively. The detection and recording are performed through the camera 10, and then the results are displayed through the display screen 11, so that the molecular markers can be quickly detected.

[0038] The driving structure includes an electric cylinder 13 and a motor 55 fixed to the lower side of the detection box 1. The output end of the electric cylinder 13 is fixed with a rotating head 14, and the rotating head 14 is rotatably connected to the rotating frame 12. The transmission structure includes a first rotating shaft 20 and multiple second rotating shafts 21 that rotatably cooperate with the bottom of the detection box 1. The first rotating shaft 20 is fixedly connected to the output end of the motor 55. A first gear 16 is fixed on the first rotating shaft 20, and a second gear 17 is fixed on the second rotating shaft 21. A third gear 18 and a fourth gear 19 are installed on the transmission cylinder 15. The third gear 18 is fixedly connected to the transmission cylinder 15, and the fourth gear 19 is rotatably connected to the transmission cylinder 15. Multiple positioning rods 22 are fixed to the bottom of the detection box 1, and multiple positioning grooves 23 are opened on the lower side of the third gear 18, and the positioning grooves 23 correspond to the positioning rods 22.

[0039] Specifically, when the position of the breeding test tube 32 needs to be changed, the electric cylinder 13 is started, so that the electric cylinder 13 drives the rotating frame 12 to move, so that the third gear 18 is engaged with the first gear 16 and the second gear 17. At this time, the motor 55 is started, so that the motor 55 drives the first rotating shaft 20 to rotate, so that the first rotating shaft 20 drives the first gear 16 to rotate, and the first gear 16 is engaged and rotated with the third gear 18, thereby driving the rotating frame 12 to rotate, so that the position of the breeding test tube 32 on the rotating frame 12 can be adjusted, so that different operations can be performed on the breeding test tube 32, making the device more flexible to work; and the rotating frame 12 can be driven by the electric cylinder 13 to adjust to different positions, so that the rotation of the rotating frame 12, the rotation of the breeding test tube 32 and the stirring, adding reagents and other operations in the breeding test tube 32 can be controlled, and multiple breeding test tubes 32 can perform different operations respectively, thereby improving the working efficiency of the device, making the device more flexible to use, thereby expanding the scope of application of the device.

[0040] The first plug-in block 24 is fixed on the upper side of the first rotating shaft 20 and the second rotating shaft 21. The mounting structure includes a plurality of mounting outer cylinders 25 mounted on the rotating frame 12. The rotating frame 12 is provided with a fixing groove 28. The mounting outer cylinder 25 is fixed in the fixing groove 28. The mounting inner cylinder 26 is fixed in the mounting outer cylinder 25. The breeding structure includes a breeding test tube 32. The breeding test tube 32 corresponds to the mounting inner cylinder 26. A card slot 56 is provided on the circumference of the breeding test tube 32. A card block 57 is rotatably matched in the mounting inner cylinder 26. The card block 57 corresponds to the card slot 56. The card block 57 and the card slot 56 are engaged. The grooves 56 are all annular structures, and the block 57 is made of elastic material. A through groove 27 is provided in the mounting outer cylinder 25 and the rotating frame 12. The through groove 27 corresponds to the first rotating shaft 20 and the second rotating shaft 21. The connecting structure includes a first rotating block 30 that is rotatably matched with the mounting inner cylinder 26. A first slot 29 is provided in the first rotating block 30. The first slot 29 corresponds to the first plug block 24. A second plug block 31 is fixed on the first rotating block 30. The bottom of the breeding test tube 32 is rotatably matched with a second rotating block 33. A second plug block 33 is provided in the second rotating block 33. The second slot 34 corresponds to the second plug block 31, and a piston 41 is fixed on the second rotating block 33. A groove 35 is provided in the second rotating block 33, and a first slide groove 39 is provided on both sides of the groove 35. The first slide groove 39 is slidably fitted with an insertion rod 36. A plurality of third slots 37 are provided at the bottom of the breeding test tube 32, and a fourth spring is fixed in the third slot 37. The third slot 37 corresponds to the insertion rod 36, and a push block 40 is slidably fitted in the groove 35. A first spring 38 is fixed between the push block 40 and the groove wall of the groove 35. The push block 40 is located between the two The bottom of the push block 40 is a conical structure between the insertion rods 36. The stirring structure includes a plurality of telescopic rods 42 fixed in the detection box 1. The telescopic rods 42 include a first rod body 43 and a second rod body 44. A second slide groove 45 is provided in the first rod body 43. The second rod body 44 slides in the second slide groove 45. A plurality of second springs 46 are fixed between the second rod body 44 and the groove wall of the second slide groove 45. The end of the second rod body 44 is rotatably matched with an electromagnet 50. The electromagnet 50 corresponds to the push block 40. The electromagnet 50 can use an electromagnet structure with its own battery.

[0041] Specifically, when centrifugation is required, the electric cylinder 13 is started, so that the electric cylinder 13 drives the rotating frame 12 to move downward, so that the fourth gear 19 is engaged with the first gear 16 and the second gear 17, and the first plug 24 is inserted into the first slot 29. At this time, the motor 55 is started, so that the motor 55 drives the first gear 16 to rotate, and the first gear 16 is engaged and rotated with the fourth gear 19, so that the fourth gear 19 is engaged and rotated with the second gear 17, so that the second rotating shaft 21 is rotated, so that the first rotating shaft 20 and the second rotating shaft 21 are rotated. The first rotating block 30 can be driven to rotate, and the first rotating block 30 drives the breeding test tube 32 to rotate through the second rotating block 33, so that the reagent in the breeding test tube 32 can be centrifuged, thereby making the reagent in the breeding test tube 32 stratified, thereby facilitating the subsequent rapid detection of the reagent in the breeding test tube 32; when stirring is required, the breeding test tube 32 is pushed to the top by the electric cylinder 13, so that the telescopic rod 42 is inserted into the breeding test tube 32, and then the electromagnet 50 is energized so that the electromagnet 50 adsorbs the push block 40, and the push block 40 is pushed. The block 40 slides upward relatively, and the fourth spring rebounds to push the insertion rod 36 completely into the third slot 37 until the pushing block 40 is limited, and the electromagnet 50 moves downward relatively, thereby driving the second rod body 44 to move downward relatively, and the stirring rod 51 that rotates and cooperates with the end of the second rod body 44 enters the breeding test tube 32. At this time, the pushing block 40 is adsorbed and fixed with the electromagnet 50, and then the rotating frame 12 is driven downward by the electric cylinder 13. At this time, the second rod body 44 moves downward synchronously until the fourth gear 19 is engaged with the first gear 16 and the second gear 17. At this time, the motor 55 can drive the first rotating block 30 to rotate, and the first rotating block 30 drives the second rotating block 33 to rotate, thereby driving the stirring rod 51 to rotate, thereby stirring. At this time, no centrifugal work is performed. When multiple breeding test tubes 32 are used, one of them can be adsorbed and fixed by the electromagnet 50, and the others are not adsorbed. At this time, different breeding test tubes 32 can be stirred or centrifuged respectively, so that the device can perform different operation steps, making the device more flexible to use and greatly improving the working efficiency of the device.

[0042] The cleaning structure includes multiple water tanks 4 fixed on the detection box 1, a water pump 6 is fixed in the water tank 4, a water channel 47 is opened in the second rod body 44, the water outlet of the water pump 6 is connected to the water channel 47, the water inlet of the water pump 6 is connected to the water tank 4, a water outlet 48 is opened on one side of the water channel 47, a second electric valve 49 is installed in the water outlet 48, the sealing structure includes a sealing cover 58 fixed on the breeding test tube 32, a push plate 62 is installed on the sealing cover 58, a sealing block 63 is installed in the sealing cover 58, an addition port 59 is opened in the sealing block 63, a blocking plate 61 is rotatably fitted in the addition port 59, a torsion spring is installed between the addition port 59 and the blocking plate 61, a push rod 54 is fixed to the lower side of the push plate 62, and multiple third springs 60 are fixed between the push plate 62 and the sealing cover 58.

[0043] Specifically, when it is necessary to clean the stirring rod 51, a breeding test tube 32 without reagents can be used as a cleaning tube. The breeding test tube 32 is pushed upward, and the push plate 62 is limited by the top cover fixed by the detection box 1, thereby pushing the push rod 54 downward relatively. The push rod 54 pushes the blocking plate 61 downward relatively, so that the blocking plate 61 rotates, and the telescopic rod 42 can be sent into the breeding test tube 32. Start the water pump 6 and the second electric valve 49 to open the water spray port 48, so that water is sprayed into the cleaning pipe through the water spray port 48. At this time, the stirring rod rotates to clean. In order to ensure the cleaning effect, a water pump and a water supply pump can also be installed in the water tank 4, and the water channel 47 is divided into a water inlet channel and a water supply channel, and the water tank 4 is divided into two cavities, so that water is supplied and pumped by the two water pumps, and the cleaning of the breeding test tube 32 can be achieved, thereby ensuring the cleanliness of the breeding test tube 32 and the stirring structure, preventing contamination of the reagents, and improving the accuracy of the device.

[0044] Working process: The breeding test tube 32 is clamped and fixed in the mounting inner cylinder 26. When the position of the breeding test tube 32 needs to be changed, the electric cylinder 13 is started, so that the electric cylinder 13 drives the rotating frame 12 to move, so that the third gear 18 is engaged with the first gear 16 and the second gear 17. At this time, the motor 55 is started, so that the motor 55 drives the first rotating shaft 20 to rotate, so that the first rotating shaft 20 drives the first gear 16 to rotate, and the first gear 16 is engaged and rotated with the third gear 18, thereby driving the rotating frame 12 to rotate, and the position of the breeding test tube 32 on the rotating frame 12 can be adjusted; when it is necessary to add reagents, the block 53 is opened, and the first test tube 32 can be fed into the first test tube through the feed port 52. Reagents are added to the reagent box 2 and the second reagent box 3. When reagents need to be added to the breeding test tube 32, the first electric valve 8 is started to open, so that the reagents in the first reagent box 2 or the second reagent box 3 flow out from the adding tube 7 and enter, and the reagents can be added; when centrifugation is required, the electric cylinder 13 is started, so that the electric cylinder 13 drives the rotating frame 12 to move downward, so that the fourth gear 19 is engaged with the first gear 16 and the second gear 17, and the first plug 24 is inserted into the first slot 29. At this time, the motor 55 is started, so that the motor 55 drives the first gear 16 to rotate, and the first gear 16 is engaged and rotated with the fourth gear 19, so that the fourth gear The wheel 19 meshes with the second gear 17 and rotates, causing the second rotating shaft 21 to rotate, so that the rotation of the first rotating shaft 20 and the second rotating shaft 21 can drive the first rotating block 30 to rotate, and the first rotating block 30 drives the breeding test tube 32 to rotate through the second rotating block 33, so that the reagent in the breeding test tube 32 can be centrifuged, thereby making the reagent in the breeding test tube 32 stratified; when stirring is required, the breeding test tube 32 is pushed to the top by the electric cylinder 13, so that the telescopic rod 42 is inserted into the breeding test tube 32, and then the electromagnet 50 is energized, so that the electromagnet 50 adsorbs the push block 40, and the push block 40 slides upward relatively, and the fourth spring rebounds to push the insert The rod 36 is completely inserted into the third slot 37 until the push block 40 is limited and the electromagnet 50 moves downward relatively, thereby driving the second rod body 44 to move downward relatively. The stirring rod 51 that rotates and cooperates with the end of the second rod body 44 enters the breeding test tube 32. At this time, the push block 40 is adsorbed and fixed with the electromagnet 50, and then the rotating frame 12 is driven downward by the electric cylinder 13. At this time, the second rod body 44 moves downward synchronously until the fourth gear 19 is engaged with the first gear 16 and the second gear 17. At this time, the motor 55 can drive the first rotating block 30 to rotate, and the first rotating block 30 drives the second rotating block 33 to rotate, thereby driving the stirring rod 51 to rotate, thereby stirring;When testing is required, multiple cameras 10 can be used to monitor the reagents in different breeding tubes 32. The breeding tubes 32 are made of transparent material, so the color changes in the breeding tubes 32 can be observed more intuitively. The camera 10 records the test results and then displays them on the display screen 11, allowing rapid detection of molecular markers.

[0045] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. A rapid detection device for molecular markers for animal husbandry molecular breeding, comprising a detection box (1), characterized in that: The upper side of the detection box (1) is equipped with a reagent adding structure and a cleaning structure, the detection box (1) is equipped with a detection structure, a stirring structure and a rotating structure, the stirring structure corresponds to the cleaning structure, the bottom of the detection box (1) is equipped with a transmission structure, the bottom of the detection box (1) is equipped with a driving structure, the driving structure corresponds to the transmission structure, the transmission structure corresponds to the stirring structure, the rotating structure is equipped with a mounting structure, the mounting structure is equipped with a breeding structure, a connecting structure is installed between the mounting structure and the breeding structure, the connecting structure corresponds to the stirring structure and the transmission structure, the top of the breeding structure is equipped with a sealing structure, the sealing structure corresponds to the reagent adding structure and the stirring structure.

2. The rapid detection device for molecular markers for animal husbandry molecular breeding according to claim 1, characterized in that: The reagent adding structure comprises a first reagent box (2) and a second reagent box (3) fixed on the detection box (1); the first reagent box (2) and the second reagent box (3) have the same structure; the tops of the first reagent box (2) and the second reagent box (3) are both provided with a feed port (52); a blocking block (53) is fixed in the feed port (52); the bottoms of the first reagent box (2) and the second reagent box (3) are both fixed with an adding pipe (7); the adding pipe (7) is located in the detection box (1); and a first electric valve (8) is installed in the adding pipe (7).

3. The rapid detection device for molecular markers for animal husbandry molecular breeding according to claim 1, characterized in that: The detection structure comprises a fixed rod (9) fixed in the detection box (1), a plurality of cameras (10) are fixed on the circumference of the fixed rod (9), a display screen (11) is fixed on the upper side of the detection box (1), the display screen (11) corresponds to the camera (10), and the rotation structure comprises a rotating frame (12) installed in the detection box (1), and a transmission cylinder (15) is fixed on the lower side of the rotating frame (12).

4. The rapid detection device for molecular markers for animal husbandry molecular breeding according to claim 3, characterized in that: The driving structure comprises an electric cylinder (13) and a motor (55) fixed to the lower side of the detection box (1); a rotating head (14) is fixed to the output end of the electric cylinder (13); the rotating head (14) is rotatably connected to the rotating frame (12); the transmission structure comprises a first rotating shaft (20) and a plurality of second rotating shafts (21) rotatably engaged with the bottom of the detection box (1); the first rotating shaft (20) is fixedly connected to the output end of the motor (55).

5. The rapid detection device for molecular markers for animal husbandry molecular breeding according to claim 4, characterized in that: A first gear (16) is fixed on the first rotating shaft (20), a second gear (17) is fixed on the second rotating shaft (21), a third gear (18) and a fourth gear (19) are installed on the transmission cylinder (15), the third gear (18) is fixedly connected to the transmission cylinder (15), and the fourth gear (19) is rotatably connected to the transmission cylinder (15), a plurality of positioning rods (22) are fixed on the bottom of the detection box (1), a plurality of positioning grooves (23) are opened on the lower side of the third gear (18), and the positioning grooves (23) correspond to the positioning rods (22).

6. The rapid detection device for molecular markers for animal husbandry molecular breeding according to claim 4, characterized in that: A first plug block (24) is fixed on the upper side of each of the first rotating shaft (20) and the second rotating shaft (21); the mounting structure comprises a plurality of mounting outer cylinders (25) mounted on the rotating frame (12); a fixing groove (28) is provided on the rotating frame (12); the mounting outer cylinders (25) are fixed in the fixing groove (28); an mounting inner cylinder (26) is fixed in the mounting outer cylinder (25); and the breeding structure comprises a breeding test tube (32); the breeding test tube (32) corresponds to the mounting inner cylinder (26).

7. The rapid detection device for molecular markers for animal husbandry molecular breeding according to claim 6, characterized in that: A clamping groove (56) is provided on the peripheral side of the breeding test tube (32), and a clamping block (57) is installed in the inner cylinder (26) for rotation. The clamping block (57) corresponds to the clamping groove (56), and both the clamping block (57) and the clamping groove (56) are annular structures. The clamping block (57) is made of elastic material.

8. The rapid detection device for molecular markers for animal husbandry molecular breeding according to claim 6, characterized in that: The mounting outer cylinder (25) and the rotating frame (12) are both provided with a through groove (27), the through groove (27) corresponding to the first rotating shaft (20) and the second rotating shaft (21), the connecting structure comprising a first rotating block (30) rotatably engaged with the mounting inner cylinder (26), a first slot (29) being provided in the first rotating block (30), the first slot (29) corresponding to the first inserting block (24), a second inserting block (31) being fixed on the first rotating block (30), a second rotating block (33) being rotatably engaged with the bottom of the breeding test tube (32), a second slot (34) being provided in the second rotating block (33), the second slot (34) corresponding to the second inserting block (31).

9. The rapid detection device for molecular markers for animal husbandry molecular breeding according to claim 8, characterized in that: A piston (41) is fixed on the second rotating block (33), a groove (35) is provided in the second rotating block (33), first sliding grooves (39) are provided on both sides of the groove (35), an insertion rod (36) is slidably fitted in the first sliding groove (39), a plurality of third slots (37) are provided at the bottom of the breeding test tube (32), the third slots (37) correspond to the insertion rod (36), a push block (40) is slidably fitted in the groove (35), a first spring (38) is fixed between the push block (40) and the groove wall of the groove (35), and the push block (40) is located between the two insertion rods (36) The bottom of the push block (40) is a conical structure, and the stirring structure includes a plurality of telescopic rods (42) fixed in the detection box (1), the telescopic rods (42) include a first rod body (43) and a second rod body (44), a second slide groove (45) is provided in the first rod body (43), the second rod body (44) is slidably fitted in the second slide groove (45), a plurality of second springs (46) are fixed between the second rod body (44) and the groove wall of the second slide groove (45), and the end of the second rod body (44) is rotatably fitted with an electromagnet (50), and the electromagnet (50) corresponds to the push block (40).

10. The rapid detection device for molecular markers for animal husbandry molecular breeding according to claim 9, characterized in that: The cleaning structure comprises a plurality of water tanks (4) fixed on the detection box (1), a water pump (6) is fixed in the water tank (4), a water channel (47) is provided in the second rod body (44), the water outlet of the water pump (6) is connected to the water channel (47), the water inlet of the water pump (6) is connected to the water tank (4), a water outlet (48) is provided on one side of the water channel (47), a second electric valve (49) is installed in the water outlet (48), and the sealing structure comprises a valve fixed to the breeding test tube (3 2) on the sealing cover (58), a push plate (62) is installed on the sealing cover (58), a sealing block (63) is installed in the sealing cover (58), an addition port (59) is opened in the sealing block (63), a blocking plate (61) is rotatably fitted in the addition port (59), a torsion spring is installed between the addition port (59) and the blocking plate (61), a push rod (54) is fixed on the lower side of the push plate (62), and a plurality of third springs (60) are fixed between the push plate (62) and the sealing cover (58).

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