Special bio-organic fertilizer for hawksbill peach based on animal manure and preparation method thereof

Abstract

The present application relates to the field of fertilizer, and particularly relates to special biological organic fertilizer for hawk mouth honey peach based on animal manure and a preparation method thereof, which comprises the following raw materials in parts by weight: animal manure compound 65-95 parts, modified peach branch pomace mixture 10-18 parts, microbial agent 2-4 parts, and mineral additive 1-2 parts; the animal manure compound is prepared by stirring chicken manure, cow manure and sheep manure with bone meal, humic acid, amino acid and other materials in stages, and then gradient fermentation at 40-60 DEG C and addition of yeast extract; the modified peach branch pomace mixture is obtained by enzymolysis of peach branches and pomace, microwave-ultrasonic synergistic treatment and modification of calcium peroxide; during preparation, chelating liquid spraying and microbial agent inoculation in stages are adopted, and efficient composting is realized through three-stage temperature control. The present application makes full use of waste such as animal manure and peach branch pomace, improves nutrient activity through modification, adapts to the growth requirement of hawk mouth honey peach, can improve soil and improve fruit quality, and has environmental protection and practical value.

Description

Technical Field

[0001] This invention relates to the field of fertilizers, and more particularly to a bio-organic fertilizer specifically for Yingzui peaches based on animal manure and its preparation method. Background Technology

[0002] In current agricultural development, the use of organic fertilizers is receiving increasing attention because they can not only improve soil fertility but also reduce the use of chemical fertilizers, thus promoting sustainable agricultural development. However, the research and application of bio-organic fertilizers made from animal manure in the market still have certain limitations, especially in the area of ​​specialized fertilizers for specific crops (such as Yingzui peaches), which still require further exploration and optimization.

[0003] Chinese Patent Application No. CN202211064481.3 discloses a bio-organic fertilizer, its preparation method, and its application, comprising the following components by weight: 8-12 parts of calcium-selenium modified carbon-based material; 200-240 parts of animal manure; 15-20 parts of humic acid; 0.5-2 parts of BGB compound bacteria; and 20-30 parts of mineral powder. The BGB compound bacteria are selected from at least two of Bacillus licheniformis, Bacillus amyloliquefaciens, and Bacillus subtilis. The bio-organic fertilizer provided by this invention can effectively increase rice yield and effectively reduce the content of available cadmium in the soil and the cadmium content in brown rice, thereby improving the production safety of rice cultivation. Chinese Patent Application No. CN202210597904.1 discloses a bio-organic fertilizer based on garden waste and its preparation method. The method includes the following steps: (1) fermenting and culturing *Streptomyces hygrophila* to obtain a bacterial inoculum, which is then combined with a mixture of wheat bran and cornmeal to produce a solid composting agent 1; (2) fermenting and culturing *Aspergillus japonicus* to produce a composting agent 2; (3) collecting and crushing garden waste, adding fresh animal manure and / or mushroom residue after crushing, mixing thoroughly, and then adding the solid composting agent 1; during the composting process, when the temperature of the compost pile drops to 50°C, adding the composting agent 2, continuing to stir, and lowering the temperature to below 35°C in the second stage of composting to obtain a composted product; (4) uniformly spraying *Bacillus sicca* inoculum onto the composted product, mixing, drying, and crushing to obtain the bio-organic fertilizer. The bio-organic fertilizer prepared by this invention can promote plant growth and / or prevent diseases. However, the bio-organic fertilizers prepared by the two inventions mentioned above have relatively low nutrient content. This lack of nutrition directly leads to problems such as yellowing, excessive growth, or poor reproductive development in plants during their growth process, which in turn affects the yield and quality of crops.

[0004] Therefore, it is extremely important to develop a new type of bio-organic fertilizer and its preparation method that combines animal manure resources with the growth requirements of Yingzui peaches, so as to meet the actual needs of agricultural production and promote the green and sustainable development of agriculture. Summary of the Invention

[0005] To address the aforementioned problems, this invention provides a bio-organic fertilizer specifically for Yingzui peaches based on animal manure and its preparation method. Animal manure, peach branch residue, and other waste materials are modified to obtain modified raw materials rich in active nutrients. These modified raw materials are then mixed with microbial agents and mineral additives in specific proportions, and through various preparation processes, a specialized bio-organic fertilizer suitable for Yingzui peach growth is obtained. This organic fertilizer effectively improves the soil, achieving a combination of waste resource utilization and crop-specific fertilizer.

[0006] The technical solution adopted by the present invention to achieve the above objectives is as follows:

[0007] The Yingzui Peach-Specific Bio-Organic Fertilizer based on Animal Manure includes the following raw materials in parts by weight: 65-95 parts animal manure complex, 10-18 parts modified peach branch and fruit residue mixture, 2-4 parts microbial agent, and 1-2 parts mineral additive.

[0008] The preparation method of the animal feces complex is as follows:

[0009] Step S1: Add bone meal and some humic acid to the pretreated animal manure and stir for 30-60 minutes. Then add amino acids, seaweed extract, citric acid and the remaining humic acid and stir for 40-60 minutes to obtain product 1.

[0010] Step S2: Product 1 is subjected to gradient heat treatment under closed conditions. First, it is kept at 40-50℃ for 9-10 hours, then the temperature is increased to 50-60℃ and kept for 15-16 hours, and then the temperature is lowered to 40-50℃ and kept for 15-20 hours. The humidity is maintained at 65%-70% throughout the process. In the last two hours of the gradient heat treatment, yeast paste is added to Product 1 and stirred for 10-20 minutes. Then it is allowed to stand for 2-2.5 hours to obtain animal fecal complex.

[0011] Further, the animal manure mentioned in step S1 is composed of chicken manure, cow manure, and sheep manure in a weight ratio of 6-8:4-6:3-5, and the mass ratio of bone meal, humic acid, pretreated animal manure, amino acids, seaweed extract, and citric acid is 0.04-0.065:0.15-0.2:1:0.065-0.1:0.04-0.08:0.006-0.01.

[0012] Furthermore, in step S2, the mass ratio of product 1 to yeast extract is 1:0.01-0.03.

[0013] The modified peach branch pomace mixture is prepared as follows:

[0014] Step a: Add the pretreated peach branch and fruit residue mixture to deionized water, then add cellulase, ligninase and laccase, and enzymatically hydrolyze at a constant temperature of 55-60℃ and pH of 4.8-5.2 for 12-14 hours to obtain the enzymatically hydrolyzed material;

[0015] Step b: The enzymatically hydrolyzed material is first microwaved at 550-600W for 5-10 minutes, and then ultrasonically treated at 250-350W and 55-65℃ for 15-25 minutes to obtain microwave-ultrasonic material;

[0016] Step c: Add calcium peroxide to the microwave-ultrasonic material and stir evenly. Then let it stand at 45-55℃ for 5.5-6.5 hours, adjust the pH value to 6.0-7.0, and dry it to obtain the final product.

[0017] Further, in step a, the mass-to-volume ratio of the pretreated peach branch and fruit pomace mixture to deionized water is 1-1.5:5 g / mL, and the mass ratio of the pretreated peach branch and fruit pomace mixture, cellulase, ligninase, and laccase is 1:0.005-0.01:0.003-0.005:0.002-0.005.

[0018] Furthermore, in step c, the mass ratio of calcium oxide to microwave-ultrasonic material is 0.015-0.02:1.

[0019] The microbial agent is composed of Bacillus subtilis, Bacillus licheniformis, and photosynthetic bacteria.

[0020] The mineral additive consists of borax, zinc sulfate, and ammonium molybdate.

[0021] This invention also provides a method for preparing a special bio-organic fertilizer for Yingzui peaches based on animal manure, comprising the following steps:

[0022] (1) Stir the animal feces complex and modified peach branch pomace mixture for 45-60 min to obtain mixture 1. Dissolve the mineral additive in the amino acid solution first, then add disodium ethylenediaminetetraacetate. Stir at 40-50℃ for 40-50 min to obtain chelate solution. Add the chelate solution to mixture 1 to obtain mixture 2.

[0023] (2) Activate the compound microbial agent in sterile glucose water at 25-35℃ for 1.5-2.5h, then add it to the mixture 2 at 10-15min intervals to obtain the inoculated material, and then compost, ferment and granulate it to obtain the final product.

[0024] The present invention has the following beneficial effects:

[0025] This invention introduces an animal manure complex that, through synergistic treatment with specific ratios of bone meal, humic acid, amino acids, and other excipients, combined with temperature gradient control and yeast extract activation, maximizes the release of basic nutrients such as nitrogen, phosphorus, and potassium from the animal manure. Simultaneously, it enriches bioactive substances such as humic acid and amino acids, enhancing nutrient availability. The modified peach branch and fruit pomace mixture, through enzymatic hydrolysis (cellulase, ligninase, etc.), microwave-ultrasound combined treatment, and calcium peroxide treatment, effectively decomposes recalcitrant components such as cellulose and lignin in the peach branch and fruit pomace, transforming them into easily absorbed small-molecule organic matter, further supplementing the organic matter and nutrients in the organic fertilizer. Mineral additives, after chelation treatment with amino acids and disodium EDTA, are added via spray, significantly improving the stability and absorption rate of trace elements such as boron, zinc, and molybdenum, meeting the specific trace element requirements for Yingzui peach growth. This organic fertilizer utilizes a microbial agent composed of Bacillus subtilis, Bacillus licheniformis, and photosynthetic bacteria in a specific ratio. After activation, it is added in stages, effectively multiplying during composting and fermentation to further decompose organic matter, release nutrients, and promote root growth. Rich in organic matter and fully decomposed, this fertilizer slowly releases nutrients upon application to the soil, providing long-lasting fertilization. Simultaneously, it improves soil structure, enhances water and fertilizer retention, and increases soil aeration, creating a suitable soil environment for Yingzui peach growth. Using animal manure (chicken, cow, and sheep manure) and peach branches and fruit residue from cultivation as primary raw materials, it achieves resource utilization of agricultural waste while ensuring safety and hygiene. This reduces environmental pollution caused by waste accumulation, lowers the production cost of organic fertilizer, and aligns with the concept of green agriculture. Detailed Implementation

[0026] The technical solutions in the embodiments of this application will be clearly and completely described below with reference to the embodiments of this application. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0027] All raw materials used in the following examples are commercially available products. Bone meal was bovine bone meal, with nitrogen 1.4%, phosphorus 1.9%, potassium 0.7%, and an effective content of 20%, purchased from Hebei Xiong'an Xinying Technology Co., Ltd.; humic acid had an effective ingredient content of 90%, grade A, purchased from Hebei Gushi Biotechnology Co., Ltd.; amino acids were compound amino acid powder, with a total nutrient content ≥99%, an effective ingredient content of 20-45%, amino acids 34.5%, nitrogen 18.3%, phosphorus 1%, potassium 1%, and organic matter 31.8%, purchased from Xuzhou Green Source Agricultural Technology Co., Ltd.; seaweed extract, with a particle size of 80-120 mesh, purchased from Sichuan Century Star Biotechnology Co., Ltd.; yeast extract had an ash content ≤15%, an effective ingredient content of 99%, and total nitrogen ≥11%, purchased from Jinan Jibin Chemical Co., Ltd.; chicken manure, cow manure, and sheep manure were sourced from local farms; cellulase, with an effective ingredient content of 100-15%, was also used. The following enzymes were used: Lignase (2% active ingredient content, 0.03% arsenic content, 20,000 enzyme activity), purchased from Shandong Tongsheng Food Ingredients Co., Ltd.; Lignase (99.99% enzyme activity retention rate, 0.001% arsenic content, 10,000 enzyme activity), purchased from Shandong Nuojie Biotechnology Co., Ltd.; Laccase (99% active ingredient content), purchased from Sichuan Huanxu Biotechnology Co., Ltd.; Yingzui peach branches were obtained from agricultural waste generated during winter pruning and summer thinning in Yingzui peach orchards; fruit pomace was obtained from a local juice factory; Bacillus subtilis ZKCC number ZKCC153891; Bacillus licheniformis ZKCC number ZKCC25630; photosynthetic bacteria were Rhodopseudomonas palustris, ZKCC number ZKCC22540; Glucose (99% active ingredient content), purchased from Shanghai Yuben Chemical Technology Co., Ltd.

[0028] Example 1: A special bio-organic fertilizer for Yingzui peaches based on animal manure, comprising the following raw materials in parts by weight: 65 parts of animal manure compound, 10 parts of modified peach branch and fruit residue mixture, 2 parts of microbial agent, and 1 part of mineral additive.

[0029] The preparation method of the animal feces complex is as follows:

[0030] Step S1: Add bone meal and 50% humic acid to the pretreated animal manure and stir for 40 minutes. Then add amino acids, seaweed extract, citric acid and the remaining humic acid and stir for 50 minutes to obtain product 1. The animal manure is first pretreated. The pretreatment process refers to drying chicken manure, cow manure and sheep manure in a weight ratio of 7:5:4 to reduce the moisture content to 40%-50%. Then, it is crushed to a particle size of less than 5 mm. The mass ratio of bone meal, humic acid, pretreated animal manure, amino acids, seaweed extract and citric acid is 0.05:0.17:1:0.08:0.06:0.008.

[0031] Step S2: Product 1 is subjected to gradient heat treatment under closed conditions. First, it is kept at 45℃ for 9.5h, then the temperature is increased to 55℃ and kept at 55℃ for 15.5h, and then the temperature is lowered to 45℃ and kept at 45℃ for 18h. The humidity is maintained at 65%-70% throughout the process. In the last two hours of the gradient heat treatment, yeast extract is added to Product 1, stirred for 15min, and then allowed to stand for 2.2h to obtain animal fecal complex, wherein the mass ratio of Product 1 to yeast extract is 1:0.02.

[0032] The modified peach branch pomace mixture is prepared as follows:

[0033] Step a: Add the pretreated peach branch and fruit pomace mixture to deionized water, then add cellulase, ligninase, and laccase. Maintain constant temperature enzymatic hydrolysis at 58℃ and pH 4.8-5.2 for 13 hours to obtain the enzymatically hydrolyzed material. The pretreatment process of the peach branch and fruit pomace mixture involves cutting the Yingzui peach branches into 5-8cm segments and crushing them to a particle size of 2-3mm. The fruit pomace is directly crushed to the same particle size. The weight ratio of Yingzui peach branches to fruit pomace is 13:7. The mass-to-volume ratio of the pretreated peach branch and fruit pomace mixture to deionized water is 1.3:5 g / mL. The mass ratio of the pretreated peach branch and fruit pomace mixture, cellulase, ligninase, and laccase is 1:0.008:0.004:0.003.

[0034] Step b: The enzymatically hydrolyzed material is first microwaved at 580W for 7 minutes, and then ultrasonically treated at 300W and 60℃ for 20 minutes to obtain microwave-ultrasonic material;

[0035] Step c: Add calcium peroxide to the microwave-ultrasonic material and stir evenly. Then let it stand at 50℃ for 6 hours. Adjust the pH value to 6.0-7.0 with 1% citric acid solution. Then transfer the pH-adjusted material to a drum dryer until the moisture content of the material drops to 30%-35%. The drying temperature is 80℃, the drum speed is 10 rpm, and the mass ratio of calcium peroxide to microwave-ultrasonic material is 0.018:1.

[0036] The microbial agent is composed of Bacillus subtilis, Bacillus licheniformis, and photosynthetic bacteria in a mass ratio of 5:3:2.

[0037] The mineral additive is composed of borax, zinc sulfate, and ammonium molybdate in a mass ratio of 1:0.8:0.3.

[0038] This invention also provides a method for preparing a special bio-organic fertilizer for Yingzui peaches based on animal manure, comprising the following steps:

[0039] (1) Mix the animal feces complex and the modified peach branch pomace mixture for 45 min to obtain mixture 1. Dissolve the mineral additive in the amino acid solution first, then add disodium ethylenediaminetetraacetate and stir for 50 min at 40℃ to obtain chelate solution. Add the chelate solution to mixture 1 by spraying to obtain mixture 2. The droplet diameter is 100 μm. Spray and stir at the same time. The amount of mineral additive added in the amino acid solution is 0.2 g / mL, and the mass ratio of disodium ethylenediaminetetraacetate to mixture 1 is 0.003:1.

[0040] (2) The compound microbial agent is first activated in sterile water containing 2% glucose at 25°C and 150 rpm for 2.5 h in a shaker. Then, it is added to the mixture 2 in 4 portions at an inoculation rate of 5% at a rotation speed of 20 rpm and an interval of 10 min each time to obtain the inoculated material. Then, the compost fermentation is carried out. The pile temperature is monitored daily for the first 5 days. When the temperature rises to 55°C, the pile is turned over at a rotation speed of 30 rpm. The pile is mixed evenly to reduce the pile temperature to 45-50°C. From the 6th to the 15th day, the pile is turned over once every 2 days to maintain the pile temperature at 50-55°C. From the 16th to the 25th day, the pile is turned over once every 3 days to reduce the pile temperature to 35-40°C and enter the decomposition stage. The moisture content is 50-55% throughout the process. The decomposed material is sent to a low-temperature dryer and dried at 40-45°C until the moisture content drops below 20%. The material is then crushed using a drum crusher and passed through a 2 mm mesh screen to obtain the final product.

[0041] Example 2: A special bio-organic fertilizer for Yingzui peaches based on animal manure, comprising the following raw materials in parts by weight: 95 parts animal manure compound, 18 parts modified peach branch and fruit residue mixture, 4 parts microbial agent, and 2 parts mineral additive.

[0042] The preparation methods of the animal feces complex, the modified peach branch pomace mixture, the microbial agent, and the mineral additives are the same as in Example 1.

[0043] This invention also provides a method for preparing a special bio-organic fertilizer for Yingzui peaches based on animal manure, comprising the following steps:

[0044] (1) Mix the animal feces complex and the modified peach branch pomace mixture for 60 min to obtain mixture 1. Dissolve the mineral additive in the amino acid solution first, then add disodium ethylenediaminetetraacetate and stir for 40 min at 50℃ to obtain chelate solution. Add the chelate solution to mixture 1 by spraying to obtain mixture 2. The droplet diameter is 100 μm. Spray and stir at the same time. The amount of mineral additive added in the amino acid solution is 0.2 g / mL, and the mass ratio of disodium ethylenediaminetetraacetate to mixture 1 is 0.003:1.

[0045] (2) The compound microbial agent is first activated in sterile water containing 2% glucose at 35°C and 150 rpm for 1.5 h in a shaker. Then, it is added to the mixture 2 in 4 portions at an inoculation rate of 5% at a rotation speed of 20 rpm and an interval of 20 min each time to obtain the inoculated material. Then, the compost fermentation is carried out. The pile temperature is monitored daily for the first 5 days. When the temperature rises to 55°C, the pile is turned over at a rotation speed of 30 rpm. The pile is mixed evenly to reduce the pile temperature to 45-50°C. From the 6th to the 15th day, the pile is turned over once every 2 days to maintain the pile temperature at 50-55°C. From the 16th to the 25th day, the pile is turned over once every 3 days to reduce the pile temperature to 35-40°C and enter the decomposition stage. The moisture content is 50-55% throughout the process. The decomposed material is sent to a low-temperature dryer and dried at 40-45°C until the moisture content drops below 20%. The material is then crushed using a drum crusher and passed through a 2 mm mesh screen to obtain the final product.

[0046] Example 3: A special bio-organic fertilizer for Yingzui peaches based on animal manure, comprising the following raw materials in parts by weight: 80 parts animal manure complex, 15 parts modified peach branch and fruit residue mixture, 3 parts microbial agent, and 1.5 parts mineral additive.

[0047] The preparation methods of the animal feces complex, the modified peach branch pomace mixture, the microbial agent, and the mineral additives are the same as in Example 1.

[0048] This invention also provides a method for preparing a special bio-organic fertilizer for Yingzui peaches based on animal manure, comprising the following steps:

[0049] (1) Mix the animal feces complex and the modified peach branch pomace mixture for 50 min to obtain mixture 1. Dissolve the mineral additive in the amino acid solution first, then add disodium ethylenediaminetetraacetate and stir for 45 min at 45℃ to obtain chelate solution. Add the chelate solution to mixture 1 by spraying to obtain mixture 2. The droplet diameter is 100 μm. Spray and stir at the same time. The amount of mineral additive added in the amino acid solution is 0.2 g / mL, and the mass ratio of disodium ethylenediaminetetraacetate to mixture 1 is 0.003:1.

[0050] (2) The compound microbial agent is first activated in sterile water containing 2% glucose at 30°C and 150 rpm for 2 hours in a shaker. Then, it is added to the mixture 2 in 4 portions at an inoculation rate of 5% at a rotation speed of 20 rpm and an interval of 13 minutes each time to obtain the inoculated material. Then, the material is composted and fermented. The pile temperature is monitored daily for the first 5 days. When the temperature rises to 55°C, the pile is turned over at a rotation speed of 30 rpm. The pile is mixed evenly to reduce the pile temperature to 45-50°C. From the 6th to the 15th day, the pile is turned over once every 2 days to maintain the pile temperature at 50-55°C. From the 16th to the 25th day, the pile is turned over once every 3 days to reduce the pile temperature to 35-40°C and enter the decomposition stage. The moisture content is 50-55% throughout the process. The decomposed material is sent to a low-temperature dryer and dried at 40-45°C until the moisture content drops below 20%. The material is then crushed using a drum crusher and passed through a 2mm mesh screen to obtain the final product.

[0051] Example 4: A special bio-organic fertilizer for Yingzui peaches based on animal manure, comprising the following raw materials in parts by weight: 80 parts of animal manure complex, 15 parts of modified peach branch and fruit residue mixture, 3 parts of microbial agent, and 1.5 parts of mineral additives.

[0052] The preparation method of the animal feces complex is as follows:

[0053] Step S1: Add bone meal and 50% humic acid to the pretreated animal manure and stir for 60 minutes. Then add amino acids, seaweed extract, citric acid and the remaining humic acid and stir for 60 minutes to obtain product 1. The animal manure is first pretreated. The pretreatment process refers to drying chicken manure, cow manure and sheep manure in a weight ratio of 8:6:5 to reduce the moisture content to 40%-50%. Then, it is crushed to a particle size of less than 5 mm. The mass ratio of bone meal, humic acid, pretreated animal manure, amino acids, seaweed extract and citric acid is 0.065:0.2:1:0.1:0.08:0.01.

[0054] Step S2: Product 1 is subjected to gradient heat treatment under closed conditions. First, it is kept at 50℃ for 9 hours, then the temperature is increased to 60℃ and kept at 60℃ for 15 hours, and then the temperature is reduced to 50℃ and kept at 50℃ for 15 hours. The humidity is maintained at 65%-70% throughout the process. In the last two hours of the gradient heat treatment, yeast paste is added to Product 1, stirred for 20 minutes, and then allowed to stand for 2.5 hours to obtain animal feces complex. The mass ratio of Product 1 to yeast paste is 1:0.03.

[0055] The modified peach branch pomace mixture is prepared as follows:

[0056] Step a: Add the pretreated peach branch and fruit pomace mixture to deionized water, then add cellulase, ligninase, and laccase. Maintain constant temperature enzymatic hydrolysis at 60℃ and pH 4.8-5.2 for 12 hours to obtain the enzymatically hydrolyzed material. The pretreatment process of the peach branch and fruit pomace mixture involves cutting the Yingzui peach branches into 5-8cm segments and crushing them to a particle size of 2-3mm. The fruit pomace is directly crushed to the same particle size. The weight ratio of Yingzui peach branches to fruit pomace is 13:7. The mass-to-volume ratio of the pretreated peach branch and fruit pomace mixture to deionized water is 1.5:5 g / mL. The mass ratio of the pretreated peach branch and fruit pomace mixture, cellulase, ligninase, and laccase is 1:0.01:0.005:0.005.

[0057] Step b: The enzymatically hydrolyzed material is first microwaved at 600W for 5 minutes, and then ultrasonically treated at 350W and 65℃ for 15 minutes to obtain microwave-ultrasonic material;

[0058] Step c: Add calcium peroxide to the microwave-ultrasonic material and stir evenly. Then let it stand at 45℃ for 5.5 hours. Adjust the pH value to 6.0-7.0 with 1% citric acid solution. Then transfer the pH-adjusted material to a drum dryer until the moisture content of the material drops to 30%-35%. The drying temperature is 80℃, the drum speed is 10 rpm, and the mass ratio of calcium peroxide to microwave-ultrasonic material is 0.02:1.

[0059] The microbial inoculant and mineral additives are the same as in Example 1.

[0060] The preparation method of the special bio-organic fertilizer for Yingzui peaches based on animal manure is the same as in Example 3.

[0061] Comparative Example 1: A bio-organic fertilizer for Yingzui peaches based on animal manure, comprising the following raw materials in parts by weight: 50 parts animal manure compound, 5 parts modified peach branch and fruit residue mixture, 3 parts microbial agent, and 1.5 parts mineral additive.

[0062] The preparation method of the animal feces complex is as follows:

[0063] Step S1: Add bone meal and 50% humic acid to the pretreated animal manure and stir for 40 minutes. Then add amino acids, seaweed extract, citric acid and the remaining humic acid and stir for 50 minutes to obtain product 1. The animal manure is first pretreated. The pretreatment process refers to drying chicken manure, cow manure and sheep manure in a weight ratio of 1:1:1 to reduce the moisture content to 40%-50%. Then, it is crushed to a particle size of less than 5 mm. The mass ratio of bone meal, humic acid, pretreated animal manure, amino acids, seaweed extract and citric acid is 0.05:0.17:1:0.08:0.06:0.008.

[0064] Step S2: Product 1 is subjected to gradient heat treatment under closed conditions. First, it is kept at room temperature for 9.5 hours, then the temperature is increased to 55℃ and kept at 55℃ for 15.5 hours, and then the temperature is lowered to 45℃ and kept at 45℃ for 18 hours. The humidity is maintained at 65%-70% throughout the process. In the last two hours of the gradient heat treatment, yeast extract is added to Product 1, stirred for 15 minutes, and then allowed to stand for 2.2 hours to obtain animal fecal complex. The mass ratio of Product 1 to yeast extract is 1:0.001.

[0065] The modified peach branch pomace mixture is prepared as follows:

[0066] Step a: Add the pretreated peach branch and fruit pomace mixture to deionized water, then add cellulase, ligninase, and laccase. Maintain constant temperature enzymatic hydrolysis for 13 hours at room temperature and pH 4.8-5.2 to obtain the enzymatically hydrolyzed material. The pretreatment process of the peach branch and fruit pomace mixture involves cutting the Yingzui peach branches into 5-8cm segments and crushing them to a particle size of 2-3mm. The fruit pomace is directly crushed to the same particle size. The weight ratio of Yingzui peach branches to fruit pomace is 1:1. The mass-to-volume ratio of the pretreated peach branch and fruit pomace mixture to deionized water is 1.3:5 g / mL. The mass ratio of the pretreated peach branch and fruit pomace mixture, cellulase, ligninase, and laccase is 1:0.001:0.001:0.001.

[0067] Step b: The enzymatically hydrolyzed material is first microwaved at 580W for 1 minute, and then ultrasonically treated at 300W and 60℃ for 10 minutes to obtain microwave-ultrasonic material;

[0068] Step c: Add calcium peroxide to the microwave-ultrasonic material and stir evenly. Then let it stand at room temperature for 6 hours. Adjust the pH value to 6.0-7.0 with 1% citric acid solution. Then transfer the pH-adjusted material to a drum dryer until the moisture content of the material drops to 30%-35%. The drying temperature is 80℃, the drum speed is 10 rpm, and the mass ratio of calcium peroxide to microwave-ultrasonic material is 0.001:1.

[0069] The microbial agent is composed of Bacillus subtilis, Bacillus licheniformis, and photosynthetic bacteria in a mass ratio of 1:1:1.

[0070] The mineral additive is composed of borax, zinc sulfate, and ammonium molybdate in a mass ratio of 1:0.1:0.1.

[0071] This invention also provides a method for preparing a special bio-organic fertilizer for Yingzui peaches based on animal manure, comprising the following steps:

[0072] (1) Mix the animal feces complex and the modified peach branch pomace mixture for 50 min to obtain mixture 1. Dissolve the mineral additive in the amino acid solution first, then add disodium ethylenediaminetetraacetate and stir for 45 min at room temperature to obtain chelate solution. Add the chelate solution to mixture 1 by spraying to obtain mixture 2. The droplet diameter is 100 μm. Spray and stir at the same time. The amount of mineral additive added in the amino acid solution is 0.2 g / mL, and the mass ratio of disodium ethylenediaminetetraacetate to mixture 1 is 0.003:1.

[0073] (2) The compound microbial agent is first activated in sterile water containing 2% glucose at 30°C and 150 rpm for 2 hours in a shaker. Then, it is added to the mixture 2 in 4 portions at an inoculation rate of 5% at a rotation speed of 20 rpm and an interval of 5 minutes each time to obtain the inoculated material. Then, the material is composted and fermented. The pile temperature is monitored daily for the first 5 days. When the temperature rises to 55°C, the pile is turned over at a rotation speed of 30 rpm. The pile is mixed evenly to reduce the pile temperature to 45-50°C. From the 6th to the 15th day, the pile is turned over once every 2 days to maintain the pile temperature at 50-55°C. From the 16th to the 25th day, the pile is turned over once every 3 days to reduce the pile temperature to 35-40°C and enter the decomposition stage. The moisture content is 50-55% throughout the process. The decomposed material is sent to a low-temperature dryer and dried at 40-45°C until the moisture content drops below 20%. The material is then crushed using a drum crusher and passed through a 2mm mesh screen to obtain the final product.

[0074] Comparative Example 2: A special bio-organic fertilizer for Yingzui peaches based on animal manure, comprising the following raw materials in parts by weight: 80 parts animal manure compound, 15 parts peach branch and fruit residue mixture, 3 parts microbial agent, and 1.5 parts mineral additive.

[0075] The preparation method of the animal feces complex is as follows:

[0076] Step S1: Add bone meal and 50% humic acid to the pretreated animal manure and stir for 40 minutes. Then add amino acids, seaweed extract, citric acid and the remaining humic acid and stir for 50 minutes to obtain product 1. The animal manure is first pretreated. The pretreatment process refers to drying chicken manure, cow manure and sheep manure in a weight ratio of 7:5:4 to reduce the moisture content to 40%-50%. Then, it is crushed to a particle size of less than 5 mm. The mass ratio of bone meal, humic acid, pretreated animal manure, amino acids, seaweed extract and citric acid is 0.05:0.17:1:0.08:0.06:0.008.

[0077] Step S2: Product 1 is subjected to gradient heat treatment under closed conditions. First, it is kept at 45℃ for 9.5h, then the temperature is increased to 55℃ and kept at 55℃ for 15.5h, and then the temperature is lowered to 45℃ and kept at 45℃ for 18h. The humidity is maintained at 65%-70% throughout the process. In the last two hours of the gradient heat treatment, yeast extract is added to Product 1, stirred for 15min, and then allowed to stand for 2.2h to obtain animal fecal complex, wherein the mass ratio of Product 1 to yeast extract is 1:0.02.

[0078] The preparation method of the peach branch and fruit residue mixture is as follows: cut the Yingzui peach branches into 5-8cm segments and crush them to a particle size of 2-3mm. Crush the fruit residue directly to the same particle size. The weight ratio of Yingzui peach branches to fruit residue in the peach branch and fruit residue mixture is 13:7.

[0079] The preparation methods of the microbial inoculant, mineral additives, and the special bio-organic fertilizer for Yingzui peaches based on animal manure are the same as in Example 3.

[0080] Comparative Example 3: A special bio-organic fertilizer for Yingzui peaches based on animal manure, comprising the following raw materials in parts by weight: 80 parts animal manure, 15 parts modified peach branch and fruit residue mixture, 3 parts microbial agent, and 1.5 parts mineral additives.

[0081] The method for preparing the animal manure is as follows: chicken manure, cow manure and sheep manure in a weight ratio of 7:5:4 are dried to reduce the moisture content to 40%-50%, and then crushed to a particle size of less than 5mm using a pulverizer;

[0082] The modified peach branch pomace mixture is prepared as follows:

[0083] Step a: Add the pretreated peach branch and fruit pomace mixture to deionized water, then add cellulase, ligninase, and laccase. Maintain constant temperature enzymatic hydrolysis at 58℃ and pH 4.8-5.2 for 13 hours to obtain the enzymatically hydrolyzed material. The pretreatment process of the peach branch and fruit pomace mixture involves cutting the Yingzui peach branches into 5-8cm segments and crushing them to a particle size of 2-3mm. The fruit pomace is directly crushed to the same particle size. The weight ratio of Yingzui peach branches to fruit pomace is 13:7. The mass-to-volume ratio of the pretreated peach branch and fruit pomace mixture to deionized water is 1.3:5 g / mL. The mass ratio of the pretreated peach branch and fruit pomace mixture, cellulase, ligninase, and laccase is 1:0.008:0.004:0.003.

[0084] Step b: The enzymatically hydrolyzed material is first microwaved at 580W for 7 minutes, and then ultrasonically treated at 300W and 60℃ for 20 minutes to obtain microwave-ultrasonic material;

[0085] Step c: Add calcium peroxide to the microwave-ultrasonic material and stir evenly. Then let it stand at 50℃ for 6 hours. Adjust the pH value to 6.0-7.0 with 1% citric acid solution. Then transfer the pH-adjusted material to a drum dryer until the moisture content of the material drops to 30%-35%. The drying temperature is 80℃, the drum speed is 10 rpm, and the mass ratio of calcium peroxide to microwave-ultrasonic material is 0.018:1.

[0086] The preparation methods of the microbial inoculant, mineral additives, and the special bio-organic fertilizer for Yingzui peaches based on animal manure are the same as in Example 3.

[0087] Comparative Example 4: A special bio-organic fertilizer for Yingzui peaches based on animal manure, comprising the following raw materials in parts by weight: 80 parts animal manure, 15 parts peach branch and fruit residue mixture, 3 parts microbial agent, and 1.5 parts mineral additives.

[0088] The method for preparing the animal manure is as follows: chicken manure, cow manure and sheep manure in a weight ratio of 7:5:4 are dried to reduce the moisture content to 40%-50%, and then crushed to a particle size of less than 5mm using a pulverizer;

[0089] The preparation method of the peach branch and fruit residue mixture is as follows: cut the Yingzui peach branches into 5-8cm segments and crush them to a particle size of 2-3mm. Crush the fruit residue directly to the same particle size. The weight ratio of Yingzui peach branches to fruit residue in the peach branch and fruit residue mixture is 13:7.

[0090] The preparation methods of the microbial inoculant, mineral additives, and the special bio-organic fertilizer for Yingzui peaches based on animal manure are the same as in Example 3.

[0091] According to the methods described in the national standards NY / T 884-2012 "Bio-organic Fertilizer" and NT / T 525-2021 "Organic Fertilizer", the various indicators of Yingzui Peach Special Bio-organic Fertilizer based on animal manure were tested, and the test results are shown in Table 1.

[0092] Table 1 Performance test of Yingzui Peach-Specific Bio-Organic Fertilizer Based on Animal Manure

[0093] ;

[0094] As shown in Table 1, the organic matter, fecal coliform count, ascarid egg mortality rate, and total nutrients of the animal manure-based bio-organic fertilizers prepared in Examples 1-4 are all superior to those in Comparative Example 1. This indicates that the bio-organic fertilizers prepared in the examples have better soil improvement and continuous fertilization capacity, faster nutrient supply intensity, hygiene and safety level, and thorough harmlessness than Comparative Example 1. Therefore, it can be concluded that the formulation proposed in this invention for preparing bio-organic fertilizers specifically for Yingzui peaches is optimal.

[0095] Table 1 shows that the organic matter, fecal coliform count, ascarid egg mortality rate, and total nutrients of the bio-organic fertilizer prepared in Comparative Example 2 were all worse than those in the Example. This indicates that the overall performance of the bio-organic fertilizer prepared in this comparative example was reduced. This is because the modification process of peach branch pomace was lacking. Peach branch pomace itself is rich in plant organic matter such as cellulose, lignin, pectin, and a small amount of sugars. After enzymatic hydrolysis (decomposition of macromolecules by cellulase, etc.) and microwave-ultrasound treatment, it is converted into small-molecule organic carbon that is more easily utilized by microorganisms, providing a stable organic material basis for the organic fertilizer. The granular structure formed after the peach branch pomace is crushed can enhance the air permeability of the mixture and avoid the formation of clumps caused by the adhesion of animal manure raw materials. The composting process addresses the oxygen deficiency issue while simultaneously providing a porous microenvironment for microbial activity. This loose structure also promotes heat transfer and oxygen exchange during composting, making it easier to maintain the high-temperature composting stage of 50-55℃. This enhances the effectiveness in killing pathogens such as fecal coliforms and roundworm eggs, improving the hygiene and safety of organic fertilizer. Furthermore, it complements the nitrogen and phosphorus nutrients in animal manure compounds. The potassium and trace elements (such as calcium and magnesium) it contains enhance the comprehensiveness of the organic fertilizer's nutrients, meeting the multi-element requirements of Yingzui peaches. Moreover, peach branch and fruit residue serve as a carrier for the resource utilization of agricultural and forestry waste, achieving a closed-loop cycle of "branches, fruit residue - fertilizer - soil" in the peach orchard, reducing environmental pollution.

[0096] As shown in Table 1, the organic matter, fecal coliform count, ascarid egg mortality rate, and total nutrients of the bio-organic fertilizer prepared in Comparative Example 3 were all worse than those in the Example. This indicates that the overall performance of the bio-organic fertilizer prepared in this comparative example was reduced. This is because the modification process of animal manure was lacking. Modified animal manure is rich in protein, polypeptides, humus, etc., and can be further enhanced by adding amino acids and seaweed extracts to provide basic nutrients such as nitrogen and phosphorus for organic fertilizer. The colloidal structure and rich carbon and nitrogen sources of the animal manure complex provide a colonization and reproduction environment for functional bacteria such as Bacillus subtilis, promoting the maintenance of bacterial activity. The animal manure complex also contains a large amount of humic acid and organic acids, which can enhance the ion exchange capacity of organic fertilizer and enhance the adsorption and slow release effect of nutrients in the soil. In addition, the gradient heat preservation treatment combined with a constant humidity environment can effectively kill pathogens and parasite eggs, such as ascarid eggs, reducing the fecal coliform count and meeting the hygiene standards for organic fertilizer.

[0097] Table 1 shows that the bio-organic fertilizer prepared in Comparative Example 4 had the worst data in terms of organic matter, fecal coliform count, ascaris egg mortality rate, and total nutrients. This indicates that the overall performance of the bio-organic fertilizer prepared in this comparative example was the worst. This is because it lacked the modification processes of animal manure and peach branch pomace. The colloidal binding properties of the animal manure complex and the fibrous looseness of the modified peach branch pomace form a "flexible-rigid" composite structure. The manure colloid fills the gaps between the pomace particles, improving the material's water-holding capacity, while the pomace fiber skeleton breaks up manure agglomeration, increasing the porosity of the mixture, solving the oxygen deficiency problem during fermentation, ensuring uniform heat transfer during the high-temperature stage of composting, reducing the fecal coliform count, and increasing the ascaris egg mortality rate.

[0098] Although embodiments of this application have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of this application, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A bio-organic fertilizer specifically for Yingzui peaches based on animal manure, characterized in that, The raw materials include the following parts by weight: 65-95 parts of animal manure compound, 10-18 parts of modified peach branch pomace mixture, 2-4 parts of microbial agent, and 1-2 parts of mineral additives. The preparation method of the animal feces complex is as follows: Step S1: Add bone meal and some humic acid to the pretreated animal manure and stir for 30-60 minutes. Then add amino acids, seaweed extract, citric acid and the remaining humic acid and stir for 40-60 minutes to obtain product 1. Step S2: Product 1 is subjected to gradient heat treatment under closed conditions. First, it is kept at 40-50℃ for 9-10 hours, then the temperature is increased to 50-60℃ and kept for 15-16 hours, and then the temperature is lowered to 40-50℃ and kept for 15-20 hours. The humidity is maintained at 65%-70% throughout the process. In the last two hours of the gradient heat treatment, yeast paste is added to Product 1 and stirred for 10-20 minutes. Then it is allowed to stand for 2-2.5 hours to obtain animal feces complex. The animal manure mentioned in step S1 is composed of chicken manure, cow manure, and sheep manure in a weight ratio of 6-8:4-6:3-5, and the mass ratio of bone meal, humic acid, pretreated animal manure, amino acids, seaweed extract, and citric acid is 0.04-0.065:0.15-0.2:1:0.065-0.1:0.04-0.08:0.006-0.

01. In step S2, the mass ratio of product 1 to yeast extract is 1:0.01-0.03; The modified peach branch pomace mixture is prepared as follows: Step a: Add the pretreated peach branch and fruit residue mixture to deionized water, then add cellulase, ligninase and laccase, and enzymatically hydrolyze at a constant temperature of 55-60℃ and pH of 4.8-5.2 for 12-14 hours to obtain the enzymatically hydrolyzed material; Step b: The enzymatically hydrolyzed material is first microwaved at 550-600W for 5-10 minutes, and then ultrasonically treated at 250-350W and 55-65℃ for 15-25 minutes to obtain microwave-ultrasonic material; Step c: Add calcium peroxide to the microwave-ultrasonic material and stir evenly. Then let it stand at 45-55℃ for 5.5-6.5 hours, adjust the pH value to 6.0-7.0, and dry it to obtain the final product. The microbial agent is composed of Bacillus subtilis, Bacillus licheniformis, and photosynthetic bacteria; The mineral additive consists of borax, zinc sulfate, and ammonium molybdate. The bone meal is bovine bone meal.

2. The special bio-organic fertilizer for Yingzui peaches based on animal manure according to claim 1, characterized in that, The mass-to-volume ratio of the pretreated peach branch and fruit pomace mixture to deionized water in step a is 1-1.5:5 g / mL, and the mass ratio of the pretreated peach branch and fruit pomace mixture, cellulase, ligninase, and laccase is 1:0.005-0.01:0.003-0.005:0.002-0.

005.

3. The special bio-organic fertilizer for Yingzui peaches based on animal manure according to claim 1, characterized in that, The mass ratio of calcium oxide to microwave-ultrasonic material in step c is 0.015-0.02:

1.

4. The method for preparing the Yingzui peach-specific bio-organic fertilizer based on animal manure according to any one of claims 1-3, characterized in that, Includes the following steps: (1) Stir the animal feces complex and modified peach branch pomace mixture for 45-60 min to obtain mixture 1. Dissolve the mineral additive in the amino acid solution first, then add disodium ethylenediaminetetraacetate. Stir at 40-50℃ for 40-50 min to obtain chelate solution. Add the chelate solution to mixture 1 to obtain mixture 2. (2) Activate the microbial agent in sterile glucose water at 25-35℃ for 1.5-2.5h, then add it to the mixture 2 at 10-15min intervals to obtain the inoculated material, and then compost, ferment and granulate it to obtain the final product.