Device and method for analyzing and detecting metabolism of acid lactic acid bacteria

Abstract

The invention discloses an acid lactic acid bacteria metabolism analysis and detection device and method, and relates to the technical field of lactic acid bacteria metabolism analysis and detection.The device comprises a detection table and a mixing reaction cylinder, a fixing plate is fixedly connected to the upper portion of the detection table, and a stirring and sample injection integrated mechanism is arranged outside the fixing plate and comprises a driving motor arranged outside the fixing plate; the side, away from the driving motor, of the transmission rod is in transmission connection with a stirring fan, the side, close to the driving motor, of the transmission rod is fixedly connected with a small rotating disc, a pressing plate is arranged on the side edge of the small rotating disc, a protruding rod is arranged below the side, away from the small rotating disc, of the pressing plate, and a large rotating disc is fixedly connected to the side edge of the protruding rod. Integrated linkage of stirring and feeding is achieved, synchronism of metabolic reaction and result accuracy are ensured, efficient and uniform stirring of lactic acid bacteria in the mixing reaction cylinder is achieved, local concentration gradient is eliminated, sufficient contact of the lactic acid bacteria and a substrate is promoted, and the metabolic reaction rate is increased.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of lactic acid bacteria metabolic analysis detection, and particularly relates to an acid lactic acid bacteria metabolic analysis detection device and method. BACKGROUND

[0002] Acid lactic acid bacteria are a kind of bacteria that can produce a large amount of lactic acid by utilizing fermentable carbohydrates. Lactic acid bacteria can normally play metabolic activity in the body, decompose proteins, sugars in food, synthesize vitamins, and fats. Under the action of lactic acid bacteria protease, macromolecular proteins in food are partially degraded into small molecular peptides and essential amino acids that can be directly utilized by the host, significantly improving the digestibility and biological value of food, and promoting gastrointestinal absorption. The importance of acid lactic acid bacteria metabolic analysis detection mainly lies in disease diagnosis, disease monitoring, disease prognosis evaluation, treatment guidance, and biological pharmaceutical production quality control.

[0003] In the prior art, the traditional manual method of adding substances is easily affected by the proficiency, fatigue degree or environmental interference of the operator, and the dosage and speed are difficult to accurately control, resulting in fluctuation of the metabolic reaction rate, affecting the synchronism of the metabolic reaction and the accuracy of the results, and possibly causing abnormal accumulation of metabolic products, interfering with the detection results. Therefore, an acid lactic acid bacteria metabolic analysis detection device and method need to be proposed. SUMMARY

[0004] The purpose of the present application is to solve the problem in the prior art that the traditional manual method of adding substances is easily affected by the proficiency, fatigue degree or environmental interference of the operator, and the dosage and speed are difficult to accurately control, resulting in fluctuation of the metabolic reaction rate, affecting the synchronism of the metabolic reaction and the accuracy of the results, and possibly causing abnormal accumulation of metabolic products, interfering with the detection results. Therefore, an acid lactic acid bacteria metabolic analysis detection device and method need to be proposed.

[0005] In order to achieve the above-mentioned purpose, the present application adopts the following technical solutions: The utility model provides an acid lactic acid bacteria metabolic analysis detection device, including detection stage and mixed reaction cylinder, fixedly connected with fixed plate above detection stage, the outside of fixed plate is provided with stirring sample feeding integrated mechanism, stirring sample feeding integrated mechanism includes the drive motor outside fixed plate setting, the output of drive motor is provided with transmission rod, the side away from drive motor of transmission rod is connected with stirring fan, the side close to drive motor of transmission rod is fixedly connected with small turntable, the side of small turntable is provided with pressing plate, the below of the side away from small turntable of pressing plate is provided with convex rod, the side of convex rod is fixedly connected with big turntable, the top of big turntable is connected with transmission belt, the side away from big turntable of transmission belt is connected with second connecting rod, the side away from transmission belt of second connecting rod is fixedly connected with rotating roller, the top of rotating roller is connected with transmission belt, the top of transmission belt is provided with fixed groove, drive motor is driven stirring fan to rotate and is stirred through transmission rod, transmission rod is driven pressing plate to rotate through small turntable, pressing plate is driven convex rod to rotate through rotation, the side of big turntable is provided with stirring fan group and makes big turntable to rotate eighth of the one, big turntable is driven second connecting rod to rotate through transmission belt, second connecting rod is driven transmission belt to rotate through rotating roller, transmission belt is driven fixed groove to move and pours acid lactic acid bacteria metabolism required material to the inside of mixed reaction cylinder.

[0006] The above technical solution further comprises: The fixed plate is fixedly connected with the drive motor, the mixed reaction cylinder is fixedly connected with the detection stage, the side away from the drive motor of the transmission rod is fixedly connected with a first bevel gear, the first bevel gear is meshingly connected with a second bevel gear below, the second bevel gear is fixedly connected with a first connecting rod above, the first connecting rod is fixedly connected with the stirring fan, the first connecting rod is rotatably connected with the detection stage, and the first connecting rod is rotatably connected with the mixed reaction cylinder.

[0007] The side close to the small turntable of the pressing plate is fixedly connected with a torsion bar, and the torsion bar is rotatably connected with the small turntable.

[0008] The side close to the small turntable of the transmission rod is rotatably connected with a support plate, the support plate is fixedly connected with the detection stage, and the support plate is rotatably connected with the big turntable.

[0009] The side of the detection stage is fixedly connected with a side plate above, the side plate is rotatably connected with the rotating roller, the side of the side plate is fixedly connected with an L-shaped plate, and the L-shaped plate is rotatably connected with the second connecting rod.

[0010] The inner wall of the mixed reaction cylinder is provided with a heating base layer, and the side close to the heating base layer of the stirring fan is provided with an optical probe.

[0011] The heating base layer can programmably control the heating / cooling rate (such as 0.1℃ / min), which is used to study the metabolic response (such as heat shock protein expression or organic acid production change) of lactic acid bacteria to temperature stress.

[0012] The side of the mixed reaction cylinder is fixedly connected with a vacuum pump, an oxygen-free mixed gas cylinder is arranged above the vacuum pump, the oxygen-free mixed gas cylinder is connected with the mixed reaction cylinder through a pipeline, and the top of the mixed reaction cylinder is rotatably connected with a sealing plate.

[0013] The typical proportion of the replacement gas in the oxygen-free mixed gas cylinder is 80% N2+10% CO2+10% H2 (or pure N2+CO2 mixed gas), which meets the anaerobic metabolic demand of lactic acid bacteria. N2: inert gas, maintains the total pressure and dilutes the residual oxygen, CO2: adjusts the pH buffer (such as stabilizing the medium pH at 5.5-6.0), promotes the growth of lactic acid bacteria, and H2 (optional): as an electron acceptor, supports the hydrogenase activity of certain facultative anaerobic lactic acid bacteria (such as Lactobacillus reuteri).

[0014] The side of the mixed reaction cylinder is fixedly connected with a vacuum pump, an oxygen-free mixed gas cylinder is arranged above the vacuum pump, the oxygen-free mixed gas cylinder is connected with the mixed reaction cylinder through a pipeline, and the top of the mixed reaction cylinder is rotatably connected with a sealing plate.

[0015] The side of the mixed reaction cylinder is fixedly connected with a vacuum pump, an oxygen-free mixed gas cylinder is arranged above the vacuum pump, the oxygen-free mixed gas cylinder is connected with the mixed reaction cylinder through a pipeline, and the top of the mixed reaction cylinder is rotatably connected with a sealing plate.

[0016] The use method of the acid lactic acid bacteria metabolic analysis detection device includes the following steps: Step one: after the lactic acid bacteria is added to the inside of the mixed reaction cylinder, the stirring and sampling integrated mechanism arranged outside the fixed plate is started, the driving motor in the stirring and sampling integrated mechanism starts to run, and the driving motor starts to control the stirring fan to rotate and stir in the mixed reaction cylinder after the driving motor is started; Step two: the transmission rod drives the small turntable to rotate, and the small turntable drives the pressing plate to rotate in a circle, one rotation of the pressing plate drives a group of convex rods to move, and one-eighth rotation of the convex rods drives the large turntable to rotate by one-eighth; Step three: the large turntable drives the transmission belt to rotate, the transmission belt drives the rotating roller to rotate through the second connecting rod, and the rotating roller drives the transmission belt to move and rotate during rotation; Step four: the transmission belt movable rotation drives the upper fixed groove to move, and the fixed groove moves to drive the acid lactic acid bacteria metabolism required substance to move and pour into the mixed reaction cylinder, complete the timed and quantitative stirring sampling operation.

[0017] The present application has the following beneficial effects: 10、In the present application, through the linkage design of the driving motor, transmission rod, small turntable, pressing plate, convex rod, large turntable, transmission belt, rotating roller and transmission belt, the acid lactic acid bacteria metabolism required substance is realized timed and quantitative pouring, realizes the integral linkage of stirring and feeding, ensures the synchronism and result accuracy of the metabolic reaction, realizes the efficient and uniform stirring of the lactic acid bacteria in the mixed reaction cylinder, eliminates the local concentration gradient, promotes the sufficient contact of lactic acid bacteria and substrate, and improves the metabolic reaction rate.

[0018] 11、In the present application, through the vacuum pump and oxygen-free mixed gas cylinder, the oxygen concentration in the mixed reaction cylinder is reduced to below the anaerobic bacteria survival threshold, which provides an anaerobic metabolic environment for lactic acid bacteria, avoids the pollution of aerobic bacteria and the oxidative damage of lactic acid bacteria.

[0019] 12、In the present application, the heating base layer can program the heating / cooling rate, which provides a stable environmental temperature for lactic acid bacteria metabolism, avoids the influence of temperature fluctuation on metabolic product accumulation, and the optical probe can realize online detection of pH value and lactic acid concentration based on fluorescent dye, which ensures real-time monitoring and data accuracy of the metabolic process. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 The structure diagram of the acid lactic acid bacteria metabolism analysis and detection device and method provided by the present application is shown in the figure; Figure 2 The external structure diagram in the present application is shown in the figure; Figure 3 The internal structure diagram in the present application is shown in the figure; Figure 4 The structure diagram of the acid lactic acid bacteria metabolism analysis and detection device and method provided by the present application is shown in the figure; Figure 1 The structure diagram of the acid lactic acid bacteria metabolism analysis and detection device and method provided by the present application is shown in the figure; Figure 5 The structure diagram of the acid lactic acid bacteria metabolism analysis and detection device and method provided by the present application is shown in the figure; Figure 2 The structure diagram of the acid lactic acid bacteria metabolism analysis and detection device and method provided by the present application is shown in the figure; Figure 6 The structure diagram of the acid lactic acid bacteria metabolism analysis and detection device and method provided by the present application is shown in the figure; Figure 2 The structure diagram of the acid lactic acid bacteria metabolism analysis and detection device and method provided by the present application is shown in the figure; Figure 7 The structure diagram of the acid lactic acid bacteria metabolism analysis and detection device and method provided by the present application is shown in the figure; Figure 3 The structure diagram of the acid lactic acid bacteria metabolism analysis and detection device and method provided by the present application is shown in the figure.

[0021] In the figure: 1, detection platform; 2, fixed plate; 3, drive motor; 4, transmission rod; 5, first bevel gear; 6, second bevel gear; 7, first connecting rod; 8, stirring fan; 9, mixed reaction cylinder; 10, small turntable; 11, pressing plate; 12, convex rod; 13, large turntable; 14, transmission belt; 15, second connecting rod; 16, rotating roller; 17, conveying belt; 18, fixed groove; 19, torsion rod; 20, support plate; 21, side plate; 22, L-shaped plate; 23, heating base layer; 24, optical probe; 25, vacuum pump; 26, oxygen-free mixed gas cylinder; 27, sealing plate; 28, base; 29, analysis and detection assembly; 30, connecting valve pipe; 31, display recording screen; 32, valve pipe; 33, cleaning liquid storage cylinder. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work belong to the scope of protection of the present application.

[0023] Please refer to Figure 1-Figure 7 As shown in the figure, the present application is an acid lactic acid bacteria metabolism analysis and detection device, which comprises a detection platform 1 and a mixed reaction cylinder 9. The upper part of the detection platform 1 is fixedly connected with a fixed plate 2. An integrated stirring and sampling mechanism is arranged outside the fixed plate 2. The integrated stirring and sampling mechanism comprises a drive motor 3 arranged outside the fixed plate 2. The output end of the drive motor 3 is provided with a transmission rod 4. The side of the transmission rod 4 away from the drive motor 3 is drivingly connected with a stirring fan 8. The side of the transmission rod 4 close to the drive motor 3 is fixedly connected with a small turntable 10. The side of the small turntable 10 is provided with a pressing plate 11. The side of the pressing plate 11 away from the small turntable 10 is provided below with a convex rod 12. The side of the convex rod 12 is fixedly connected with a large turntable 13. The upper part of the large turntable 13 is drivingly connected with a transmission belt 14. The side of the transmission belt 14 away from the large turntable 13 is drivingly connected with a second connecting rod 15. The side of the second connecting rod 15 away from the transmission belt 14 is fixedly connected with a rotating roller 16. The upper part of the rotating roller 16 is drivingly connected with a conveying belt 17. The upper part of the conveying belt 17 is provided with a fixed groove 18. The drive motor 3 drives the stirring fan 8 to rotate and stir through the transmission rod 4. The transmission rod 4 drives the pressing plate 11 to rotate through the small turntable 10. The pressing plate 11 drives the convex rod 12 to rotate through rotation. The convex rod 12 is provided with eight stirring fans 8 on the side of the large turntable 13 and makes the large turntable 13 rotate by one eighth. The large turntable 13 drives the second connecting rod 15 to rotate through the transmission belt 14. The second connecting rod 15 drives the conveying belt 17 to rotate through the rotating roller 16. The conveying belt 17 drives the fixed groove 18 to move and pour the substances required for acid lactic acid bacteria metabolism into the mixed reaction cylinder 9.

[0024] The fixed plate 2 is fixedly connected to the drive motor 3, the mixing reaction tube 9 is fixedly connected to the detection platform 1, the transmission rod 4 is fixedly connected to the first bevel gear 5 on the side away from the drive motor 3, the second bevel gear 6 is meshed and connected below the first bevel gear 5, and the first connecting rod 7 is fixedly connected above the second bevel gear 6. The first connecting rod 7 is fixedly connected to the stirring fan 8, the first connecting rod 7 is rotatably connected to the detection platform 1, and the first connecting rod 7 is rotatably connected to the mixing reaction tube 9.

[0025] After the worm gear 1 is in the gear 2, the worm gear 1 is in the gear 2 and the worm gear 1 is in the gear 2. When the worm gear 1 is in the gear 2, the worm gear 1 is in the gear 2 and the worm gear 1 is in the gear 2. When the worm gear 1 is in the gear 2, the worm gear 1 is in the gear 2 and the worm gear 1 is in the gear 2. When the turntable 10 rotates, it drives the pressure plate 11 to rotate in a circular manner. When the pressure plate 11 rotates one circle, it drives the convex rod 12 to move. The convex rod 12 is provided with a stirring fan 8 group on the side of the large turntable 13. The pressure plate 11 only drives the large turntable 13 to rotate one-eighth of the time for each rotation. When the large turntable 13 rotates, it drives the transmission belt 14 to rotate. When the transmission belt 14 rotates, it drives the second connecting rod 15 connected to the other side of the transmission to rotate. When the second connecting rod 15 rotates, it drives the rotating roller 16 fixedly connected to the other side of the transmission belt 14 to rotate, so that when the rotating roller 16 rotates, it drives the conveyor belt 17 to rotate in a mobile manner, and when the conveyor belt 17 moves, it drives the fixed groove 18 above to move. The substances required for the metabolism of acidic lactic acid bacteria are placed inside the fixed groove 18. The fixed groove 18 moves in stages to drive different substances to be poured into the interior of the mixing reaction cylinder 9, completing the timed and quantitative stirring and sampling operation.

[0026] In one embodiment, for the above-mentioned pressing plate 11 , a torsion bar 19 is fixedly connected to one side of the pressing plate 11 close to the small turntable 10 , and the torsion bar 19 is rotationally connected to the small turntable 10 .

[0027] In the embodiment, when the additive is not needed, the torsion bar 19 is rotated to drive the pressing plate 11 fixedly connected with the side of the torsion bar 19 to rotate, so that the pressing plate 11 is separated from the convex rod 12 and no longer drives the large disc 13 to rotate.

[0028] In one embodiment, for the transmission rod 4, a supporting plate 20 is rotationally connected to the side of the transmission rod 4 close to the small disc 10, the supporting plate 20 is fixedly connected with the detection table 1, and the supporting plate 20 is rotationally connected with the large disc 13.

[0029] In the embodiment, when the transmission rod 4 rotates, the transmission rod 4 rotates through the inside of the supporting plate 20, so that the supporting plate 20 can provide stable support for the rotation of the transmission rod 4, and when the large disc 13 rotates, the other side of the large disc 13 rotates at the side of the supporting plate 20, ensuring the stability during transmission.

[0030] In one embodiment, for the detection table 1, a side plate 21 is fixedly connected above the side of the detection table 1, the side plate 21 is rotationally connected with the rotating roller 16, an L-shaped plate 22 is fixedly connected at the side of the side plate 21, and the L-shaped plate 22 is rotationally connected with the second connecting rod 15.

[0031] In the embodiment, the side plate 21 is fixedly connected above the side of the detection table 1, the rotating roller 16 rotates at the side of the side plate 21, and the other side of the L-shaped plate 22 fixedly connected at the side of the side plate 21 is rotationally connected with the second connecting rod 15, ensuring the stability of the rotating roller 16, the transmission belt 17, the fixed groove 18, and the second connecting rod 15 during operation.

[0032] In one embodiment, for the mixing reaction cylinder 9, a heating base layer 23 is arranged on the inner wall of the mixing reaction cylinder 9, and an optical probe 24 is arranged on the side of the stirring fan 8 close to the heating base layer 23.

[0033] In the embodiment, the heating base layer 23 arranged on the inner wall of the mixing reaction cylinder 9 stabilizes the culture environment at the optimal metabolism temperature of lactic acid bacteria, such as 37℃±0.5℃, to avoid temperature fluctuations that reduce enzyme activity or inhibit metabolic pathways, and the optical probe 24 is arranged in multiple groups outside the stirring fan 8, which can realize online detection of pH value in the range of 5.5-8.0 with an accuracy of ±0.02 pH based on the protonation / deprotonation ratio change of fluorescent dyes such as HPTS, and ensure accurate monitoring of the concentration of lactic acid in complex environments.

[0034] In one embodiment, for the above-mentioned mixed reaction cylinder 9, a vacuum pump 25 is fixedly connected to the side of the mixed reaction cylinder 9, an oxygen-free mixed gas cylinder 26 is arranged above the vacuum pump 25, and the oxygen-free mixed gas cylinder 26 is connected to the mixed reaction cylinder 9 through a pipeline. A sealing plate 27 is rotatably connected to the top of the mixed reaction cylinder 9.

[0035] In this embodiment, after the stirring is completed, the inside of the mixed reaction cylinder 9 is closed by the sealing plate 27. The oxygen concentration in the mixed reaction cylinder 9 after the stirring is completed is reduced from 21% air to <0.1% anaerobic bacteria survival threshold through the oxygen-free mixed gas cylinder 26 and the first bevel gear 5 arranged on the side of the mixed reaction cylinder 9, so as to avoid contamination of aerobic bacteria and oxidative damage of lactic acid bacteria.

[0036] In one embodiment, for the above-mentioned device main body detection table 1, a base 28 is arranged on the side of the detection table 1 close to the mixed reaction cylinder 9. An analysis and detection assembly 29 is arranged above the base 28. The analysis and detection assembly 29 is fixedly connected with a connecting valve pipe 30. The connecting valve pipe 30 is fixedly connected between the analysis and detection assembly 29 and the mixed reaction cylinder 9. A display recording screen 31 is arranged above the side of the base 28 close to the analysis and detection assembly 29.

[0037] In this embodiment, the base 28 is fixedly connected above the detection table 1. The analysis and detection assembly 29 is arranged above the base 28. The lactic acid bacteria after the metabolic reaction is transmitted to the inside of the analysis and detection assembly 29 through the connecting valve pipe 30 fixedly connected between the inside of the mixed reaction cylinder 9 and the analysis and detection assembly 29. The display recording screen 31 arranged above the base 28 synchronously displays the parameters such as pH, dissolved oxygen, and metabolite concentration in the form of numbers, curves, and thermal maps, with a resolution of 0.01 pH unit and 0.1 mM concentration gradient.

[0038] In one embodiment, for the above-mentioned device main body detection table 1, a base 28 is arranged on the side of the detection table 1 close to the mixed reaction cylinder 9. An analysis and detection assembly 29 is arranged above the base 28. The analysis and detection assembly 29 is fixedly connected with a connecting valve pipe 30. The connecting valve pipe 30 is fixedly connected between the analysis and detection assembly 29 and the mixed reaction cylinder 9. A display recording screen 31 is arranged above the side of the base 28 close to the analysis and detection assembly 29.

[0039] In this embodiment, the base 28 is fixedly connected above the detection table 1. The analysis and detection assembly 29 is arranged above the base 28. The lactic acid bacteria after the metabolic reaction is transmitted to the inside of the analysis and detection assembly 29 through the connecting valve pipe 30 fixedly connected between the inside of the mixed reaction cylinder 9 and the analysis and detection assembly 29. The display recording screen 31 arranged above the base 28 synchronously displays the parameters such as pH, dissolved oxygen, and metabolite concentration in the form of numbers, curves, and thermal maps, with a resolution of 0.01 pH unit and 0.1 mM concentration gradient.

[0040] Although the embodiments of the present application have been shown and described, it is to be understood that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present application. The scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A device for analyzing and detecting the metabolism of lactic acid bacteria, comprising a detection table (1) and a mixing reaction cylinder (9), characterized in that: A fixed plate (2) is fixedly connected to the top of the detection table (1), and a stirring and sampling integrated mechanism is arranged on the outside of the fixed plate (2). The stirring and sampling integrated mechanism includes a driving motor (3) arranged on the outside of the fixed plate (2), and a transmission rod (4) is arranged at the output end of the driving motor (3). The side of the transmission rod (4) away from the driving motor (3) is transmission-connected to a stirring fan (8), and the side of the transmission rod (4) close to the driving motor (3) is fixedly connected to a small turntable (10), and a pressure plate (11) is arranged on the side of the small turntable (10), and a protruding rod (12) is arranged below the side of the pressure plate (11) away from the small turntable (10), and a large turntable (13) is fixedly connected to the side of the protruding rod (12), and a transmission belt (14) is transmission-connected above the large turntable (13), and a second connecting rod (15) is transmission-connected to the side of the transmission belt (14) away from the large turntable (13), and the second connecting rod (15) is transmission-connected to the side of the transmission belt ( A rotating roller (16) is fixedly connected to one side of the large turntable (14), and a transmission belt (17) is connected to the top of the rotating roller (16). A fixed groove (18) is provided above the transmission belt (17). The driving motor (3) drives the stirring fan (8) to rotate and stir through the transmission rod (4). The transmission rod (4) drives the pressure plate (11) to rotate through the small turntable (10). The pressure plate (11) drives the convex rod (12) to rotate by rotating. The convex rod (12) is provided with a stirring fan (8) group on the side of the large turntable (13) and causes the large turntable (13) to rotate one-eighth. The large turntable (13) drives the second connecting rod (15) to rotate through the transmission belt (14). The second connecting rod (15) drives the transmission belt (17) to rotate through the rotating roller (16). The transmission belt (17) drives the fixed groove (18) to move and pours the substances required for the metabolism of acidic lactic acid bacteria into the mixing reaction cylinder (9).

2. The acid lactic acid bacteria metabolic analysis and detection device according to claim 1, characterized in that: The fixing plate (2) is fixedly connected to the driving motor (3), the mixing reaction cylinder (9) is fixedly connected to the detection platform (1), the transmission rod (4) is fixedly connected to a first bevel gear (5) on a side away from the driving motor (3), a second bevel gear (6) is meshedly connected below the first bevel gear (5), a first connecting rod (7) is fixedly connected above the second bevel gear (6), the first connecting rod (7) is fixedly connected to the stirring fan (8), the first connecting rod (7) is rotationally connected to the detection platform (1), and the first connecting rod (7) is rotationally connected to the mixing reaction cylinder (9).

3. The acid lactic acid bacteria metabolic analysis and detection device according to claim 1, characterized in that: A torsion bar (19) is fixedly connected to one side of the pressure plate (11) close to the small turntable (10), and the torsion bar (19) is rotationally connected to the small turntable (10).

4. The acid lactic acid bacteria metabolic analysis and detection device according to claim 3, characterized in that: A support plate (20) is rotatably connected to a side of the transmission rod (4) close to the small turntable (10); the support plate (20) is fixedly connected to the detection table (1); and the support plate (20) is rotatably connected to the large turntable (13).

5. The acid lactic acid bacteria metabolic analysis and detection device according to claim 1, characterized in that: A side plate (21) is fixedly connected to the upper side of the detection platform (1), and the side plate (21) is rotatably connected to the rotating roller (16). An L-shaped plate (22) is fixedly connected to the side of the side plate (21), and the L-shaped plate (22) is rotatably connected to the second connecting rod (15).

6. The acid lactic acid bacteria metabolic analysis and detection device according to claim 1, characterized in that: The inner wall of the mixing reaction cylinder (9) is provided with a heating base layer (23), and the side of the stirring fan (8) close to the heating base layer (23) is provided with an optical probe (24).

7. The acid lactic acid bacteria metabolic analysis and detection device according to claim 1, characterized in that: The side of the mixing reaction cylinder (9) is fixedly connected to a vacuum pump (25), and an oxygen-free mixed gas cylinder (26) is provided above the vacuum pump (25). The oxygen-free mixed gas cylinder (26) and the mixing reaction cylinder (9) are connected via a pipeline, and a sealing plate (27) is rotatably connected to the top of the mixing reaction cylinder (9).

8. The acid lactic acid bacteria metabolic analysis and detection device according to claim 1, characterized in that: The detection table (1) is provided with a base (28) on a side close to the mixing reaction cylinder (9), and an analysis and detection component (29) is provided above the base (28). The analysis and detection component (29) is fixedly connected to a connecting valve tube (30), and the connecting valve tube (30) is fixedly connected to the mixing reaction cylinder (9). A display recording screen (31) is provided above the side of the base (28) close to the analysis and detection component (29).

9. The acid lactic acid bacteria metabolic analysis and detection device according to claim 8, characterized in that: A branch valve pipe (32) is provided on the side of the connecting valve pipe (30) close to the mixing reaction cylinder (9), and a cleaning liquid storage cylinder (33) is fixedly connected to the side of the branch valve pipe (32) away from the connecting valve pipe (30).

10. The method for using the acid lactic acid bacteria metabolic analysis and detection device according to claim 1, characterized in that: The following steps are involved: Step 1: After the lactic acid bacteria are added into the mixing reaction tube (9), the stirring and sampling integrated mechanism provided outside the fixed plate (2) is started, and the driving motor (3) in the stirring and sampling integrated mechanism starts to operate. After the driving motor (3) is started, the stirring fan (8) is controlled by the transmission rod (4) to rotate and stir inside the mixing reaction tube (9); Step 2: The transmission rod (4) drives the small turntable (10) to rotate, and the small turntable (10) drives the pressure plate (11) to rotate in a circle. When the pressure plate (11) rotates one circle, it drives a group of protruding rods (12) to move. When the protruding rods (12) rotate one eighth, it drives the large turntable (13) to rotate one eighth. Step 3: The large turntable (13) rotates to drive the transmission belt (14) to rotate, and the transmission belt (14) rotates through the rotating roller (16) with the second connecting rod (15), and the rotating roller (16) drives the transmission belt (17) to rotate in a mobile manner during the rotation process; Step 4: The movable rotation of the conveyor belt (17) will drive the upper fixed tank (18) to move. When the fixed tank (18) moves, it will drive the substances required for the metabolism of acid lactic acid bacteria to move and pour them into the interior of the mixing reaction cylinder (9), completing the timed and quantitative stirring and sampling operation.

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