Method for manufacturing sensitive layer of pressure sensor with high detection upper limit

Abstract

The invention discloses a method for manufacturing a sensitive layer of a high detection upper limit pressure sensor, and relates to the technical field of biological materials.The method comprises the steps that a target strain is inoculated into a sterile culture medium and cultured at the set temperature for set time, and activated target bacteria are obtained; inoculating the activated target bacteria into a liquid culture medium, and putting the liquid culture medium into a shaking table, so that oxygen is dissolved in the liquid culture medium; when the target bacteria grow to a logarithmic phase in the liquid culture medium, inducing the target bacteria to express proteins which can be combined with each other, so that an engineering bacteria adhesion mixture with a network structure is generated; according to the method, the engineering bacteria adhesion mixture with a network structure can be generated by inducing bacteria to express proteins which can be combined with each other, and the engineering bacteria adhesion mixture still has relatively high performance in a high-pressure environment and is prepared by bacterial culture, so that the cost is relatively low.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological materials, and particularly relates to a method for manufacturing a sensitive layer of a high-detection-upper-limit pressure sensor. BACKGROUND

[0002] The current high-pressure detection technology faces the following main problems in actual application:

[0003] 1. Limitation of high-pressure detection: Traditional pressure sensors are usually based on silicon or quartz materials. Although these sensors perform well in the standard pressure range, they often have problems such as reduced sensitivity and unstable signals in high-pressure environments.

[0004] 2. High manufacturing cost: High-quality pressure sensors usually have high manufacturing costs, which to some extent limits their large-scale application. Some new sensors, such as those based on nanomaterials or new composite materials, have complex preparation processes and higher costs. For example, sensors made of carbon nanotubes or graphene have excellent performance, but the manufacturing cost is high, making it difficult to achieve large-scale production and application. SUMMARY

[0005] Therefore, the embodiments of the present application provide a method for manufacturing a sensitive layer of a high-detection-upper-limit pressure sensor to improve the pressure resistance of the sensor sensitive layer and reduce the manufacturing cost.

[0006] An aspect of the embodiments of the present application provides a method for manufacturing a sensitive layer of a high-detection-upper-limit pressure sensor, which comprises the following steps:

[0007] Inoculate the target strain into a sterile culture medium and cultivate it at a set temperature for a set time to obtain activated target bacteria;

[0008] Inoculate the activated target bacteria into a liquid culture medium and place the liquid culture medium in a shaking bed to dissolve oxygen in the liquid culture medium;

[0009] When the target bacteria grow to the logarithmic growth phase in the liquid culture medium, induce the target bacteria to express proteins that can combine with each other to generate an engineered bacterial adhesion mixture with a network structure;

[0010] Use the engineered bacterial adhesion mixture to prepare a sensor sensitive layer.

[0011] In some embodiments, the method further comprises the following steps:

[0012] Inject the engineered bacterial adhesion mixture into a catheter through a syringe, add wires at both ends of the catheter respectively, and then seal both ends of the catheter with hot melt adhesive;

[0013] The two wires are connected to a power source, and the resistance of the conduit when pressure is applied is tested.

[0014] In some embodiments, the method further comprises the steps of:

[0015] Removing gas from the conduit.

[0016] In some embodiments, the inoculating the target bacteria into the sterile culture medium comprises the steps of:

[0017] Inoculating Escherichia coli as the target bacteria into the sterile culture medium.

[0018] In some embodiments, the inducing the target bacteria to express the mutually combinable proteins when the target bacteria grow to the logarithmic growth phase in the liquid culture medium comprises the steps of:

[0019] In some embodiments, the inducing the target bacteria to express the mutually combinable proteins when the target bacteria grow to the logarithmic growth phase in the liquid culture medium comprises the steps of:

[0020] The present application has at least the following advantages:

[0021] The present application can inoculate the target bacteria into the sterile culture medium, and culture at a set temperature for a set time to obtain activated target bacteria; inoculate the activated target bacteria into the liquid culture medium, and place the liquid culture medium in a shaker to dissolve oxygen in the liquid culture medium; when the target bacteria grow to the logarithmic growth phase in the liquid culture medium, induce the target bacteria to express the mutually combinable proteins to generate the engineered bacteria adhesion mixture with network structure; and use the engineered bacteria adhesion mixture to prepare the sensor sensitive layer. The present application can generate the engineered bacteria adhesion mixture with network structure by inducing the bacteria to express the mutually combinable proteins, and the engineered bacteria adhesion mixture still has high performance in a high-pressure environment, and is made of bacteria culture, so the cost is low. BRIEF DESCRIPTION OF DRAWINGS

[0022] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiment description. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0023] Figure 1A flowchart of a method for manufacturing a sensitive layer of a high-detection upper limit pressure sensor is provided for the embodiments of the present application.

[0024] Figure 2 An example flowchart of a method for manufacturing a sensitive layer of a high-detection upper limit pressure sensor is provided for the embodiments of the present application.

[0025] Figure 3 An example graph of an R-P curve is provided for the embodiments of the present application. DETAILED DESCRIPTION

[0026] In order to make the purposes, technical solutions and advantages of the present application clearer, the present application will be further described in detail below in combination with the drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and do not limit the present application.

[0027] Before the embodiments of the present application are described in detail, first, some related technologies involved in the embodiments of the present application are described as follows:

[0028] Related technologies:

[0029] 1. Industrial automation and intelligent manufacturing demand: In modern industrial production, the degree of automation and intelligentization is continuously improved, and the demand for pressure sensors capable of accurately measuring high pressure is increasing. For example, in the petroleum chemical industry, metallurgy, pharmaceutical industry and other industries, real-time pressure monitoring of high-pressure reaction kettles, pipeline systems and other equipment is required to ensure the safety and stability of the production process. High-detection upper limit pressure sensors can meet the precise measurement needs of these industrial equipment under high-pressure working conditions, and realize precise control and optimization of the production process.

[0030] 2. Technological upgrading of the automobile industry: As an important means of transportation in modern society, automobiles are constantly upgrading their technology. In the automobile engine management system, high-detection upper limit pressure sensors are needed to accurately measure fuel injection pressure, intake pressure, etc., to improve engine performance and fuel economy, while reducing exhaust emissions. In addition, in the automobile braking system, suspension system and other parts, high-detection upper limit pressure sensors are also needed to monitor pressure changes to ensure the safety and comfort of the vehicle.

[0031] 3. High precision requirements in the field of aerospace: Aerospace is a field with extremely high requirements for precision and reliability. In the monitoring of aircraft engine operation, high-detection upper limit pressure sensors can be used to measure the high-temperature and high-pressure environment inside the engine, such as combustion chamber pressure, turbine inlet pressure, etc., to ensure the normal operation and safe operation of the engine. At the same time, in the key parts of the aircraft's hydraulic system, fuel system, etc., high-detection upper limit pressure sensors are also needed for accurate pressure measurement and control to ensure the safety of the aircraft in flight.

[0032] 4. Monitoring needs in the energy and power industries: In the energy sector, such as power plants, oil pipelines, and natural gas pipelines, accurate monitoring of high-pressure gas or liquid pressure is required. High-limit pressure sensors can monitor pipeline pressure in real time, preventing safety incidents such as pipeline ruptures caused by excessive pressure, while also helping to optimize energy transmission and distribution efficiency.

[0033] 5. Technological development promotes sensor technology advancement: The continuous advancement of microelectromechanical systems (MEMS), nanotechnology, and materials science has provided advanced technical means and material foundations for the development of high-detection-limit pressure sensors. For example, MEMS technology enables the miniaturization and high-performance of sensors, enabling the miniaturization and high-performance of high-detection-limit pressure sensors to meet the space constraints and performance requirements of different application scenarios.

[0034] Other related technologies:

[0035] 1. Advances in material technology (this patent is based on improvements in materials):

[0036] (1) Application of semiconductor materials: The piezoresistive effect of semiconductor materials such as silicon is widely used in pressure sensors. By making strain gauges on silicon wafers, high-sensitivity pressure measurements can be achieved. In addition, the emergence of new semiconductor materials such as silicon carbide (SiC) enables pressure sensors to operate in harsh environments such as high temperature and high pressure, thereby improving the upper detection limit of the sensor. For example, the α(6H) SiC pressure sensor reported by Robert.S.Okojie, which was tested at 500°C, performed well under the conditions of an input voltage of 5 V and a measured pressure of 6.9 MPa.

[0037] (2) The emergence of new conductive polymer materials: Some conductive polymer materials, such as conjugated polymers, have unique electrical and mechanical properties. By designing and modifying their molecular structure, they can produce more significant electrical signal changes when subjected to pressure, thereby improving the sensitivity and detection limit of pressure sensors. For example, Zhang Jianhua et al. from the School of Microelectronics at Shanghai University proposed a doped polymer flexible pressure sensor prepared based on a sacrificial template-full solution method. Its sensitivity in the range of 0-5.4 kPa is as high as 699.8 kPa⁻¹, and by optimizing the structure and materials, it is expected to further expand its detection limit.

[0038] (3) Innovation of modulus gradient ion conductive hydrogel materials: The modulus gradient ion conductive hydrogel developed by the Ningbo Institute of Materials Technology and Engineering of the Chinese Academy of Sciences combines the polyacrylamide / phytic acid region rich in plasticizing effect and the polyacrylic acid / phytic acid region rich in microphase separation, effectively solving the problem of traditional pressure sensors that cannot simultaneously consider high sensitivity and wide pressure detection range, and realizing high sensitivity detection in a wide pressure range of 3.7 Pa to 1200 kPa.

[0039] 2. Innovation of structural design:

[0040] (1) Microstructure design: Using micro-machining technology, various microstructures such as thin films, cantilever beams, and bridge structures can be manufactured on silicon wafers, which can improve the sensitivity and upper detection limit of pressure sensors. For example, by reducing the thickness and increasing the area of the thin film, the response sensitivity of the sensor to pressure changes can be improved, thereby achieving a higher detection limit.

[0041] (2) Multi-film layer structure: The use of multi-film layer structure design can achieve stress transmission and amplification between different film layers, improving the detection limit of the sensor. For example, combining pressure-sensitive thin films with multi-layer structures such as insulating thin films and conductive thin films can optimize the performance of the sensor, enabling it to work in a higher pressure range.

[0042] (3) Special structure pressure sensors: Some special structure pressure sensors are also emerging, such as fiber Bragg grating (FBG) based pressure sensors and capacitive micro-mechanical structure based pressure sensors. These sensors have high sensitivity, high detection limit, and anti-electromagnetic interference, etc., and are suitable for different application scenarios.

[0043] 3. Development of signal processing technology:

[0044] (1) Application of high-precision analog-to-digital converters: With the development of electronic technology, high-precision analog-to-digital converters (ADCs) are constantly emerging. The use of high-precision ADCs can accurately convert the weak analog signals output by the sensor into digital signals, improving the measurement accuracy and detection limit of the sensor.

[0045] (2) Noise suppression algorithms and adaptive filtering technology: In high detection limit pressure sensors, the signal processing circuit needs to process weak sensor signals, which are easily disturbed by various noises. Therefore, the application of noise suppression algorithms and adaptive filtering technology becomes particularly important. Through these technologies, the influence of noise on sensor signals can be effectively reduced, improving the quality and reliability of signals, thereby achieving a higher detection limit.

[0046] (3) Intelligent signal processing technology: With the development of artificial intelligence and machine learning technology, intelligent signal processing technology has also begun to be applied in high-detection upper limit pressure sensors. For example, using neural network algorithms to analyze and process sensor signals can achieve self-calibration, self-adaptation, and fault diagnosis functions of the sensor, improving the performance and reliability of the sensor.

[0047] 4. Diversification of application requirements:

[0048] (1) Special requirements in the medical field: In the medical field, such as pressure monitoring of surgical instruments, detection of human physiological signals, etc., high-detection upper limit pressure sensors are required to have high precision, high reliability, good biocompatibility, etc. For example, tonometers used to measure intraocular pressure require pressure sensors with extremely small size and high sensitivity to achieve accurate measurement of intraocular pressure in the human eye.

[0049] (2) Demand for robot tactile perception: With the continuous development of robot technology, robots are increasingly widely used in industries, services, and other fields. In order to realize the tactile perception function of robots, high-detection upper limit pressure sensors are required to detect the contact pressure between the robot and the object in real time, and have high resolution and fast response capability. For example, when a robot grasps an object, the size of the grasping force is perceived through a pressure sensor to achieve stable grasping and operation of the object.

[0050] (3) Application in environmental monitoring and meteorological measurement: In environmental monitoring and meteorological measurement, high-precision measurement of atmospheric pressure, water pressure, etc. is required. High-detection upper limit pressure sensors can be used to measure pressure changes in extreme environments such as high mountains and deep seas, providing important data support for weather forecasting, ocean research, etc. For example, sensors used to measure deep-sea pressure need to withstand extremely high water pressure and have good stability and reliability.

[0051] Current high-pressure detection technology faces the following main problems in practical application:

[0052] 1. Limitations of high-pressure detection: Traditional pressure sensors are usually based on silicon or quartz materials. Although these sensors perform well in standard pressure ranges, they often exhibit reduced sensitivity and unstable signals in high-pressure environments.

[0053] 2. High manufacturing cost: High-quality pressure sensors usually have high manufacturing costs, which to some extent limits their large-scale application. Some new sensors, such as those based on nanomaterials or new composite materials, have complex preparation processes and higher costs. For example, sensors made of carbon nanotubes or graphene materials have excellent performance but high manufacturing costs, making it difficult to achieve large-scale production and application.

[0054] In view of the above shortcomings of the prior art, the present application aims to achieve the following main objectives:

[0055] 1. Improve the sensitivity and stability of high pressure detection: The present application utilizes the unique properties of engineered bacterial adhesion materials to improve the detection performance of the sensor under extremely high pressure conditions. Engineered bacterial adhesion materials can maintain stable signal output under extreme environments.

[0056] 2. Low material cost and simple preparation process: Bacteria can be mass-produced under specific culture conditions, which is relatively low-cost. Compared with the preparation process of traditional sensors, the use of bacteria to make sensors generally does not require large and expensive production equipment. It can be completed by simple steps such as culture and purification, without the need for high-precision photolithography machines, vacuum coating equipment, etc. as in semiconductor sensor manufacturing. And the bacterial growth and metabolism process is generally carried out at room temperature and normal pressure, without the need for special high temperature, high pressure or extreme environmental conditions, which not only reduces energy consumption and equipment requirements, but also simplifies the process flow and reduces production costs.

[0057] 3. Biodegradable and environmentally friendly: The sensor material made of bacteria is usually biodegradable and will not produce a large amount of waste like traditional plastics or metal materials after use, reducing waste disposal costs and the risk of environmental pollution.

[0058] Reference Figure 1 The embodiments of the present application provide a method for making a high-detection upper limit pressure sensor sensitive layer, which specifically includes the following steps S100-S130:

[0059] S100: inoculate the target strain into a sterile culture medium and incubate it at a set temperature for a set time to obtain activated target bacteria;

[0060] S110: inoculate the activated target bacteria into a liquid culture medium and place the liquid culture medium in a shaker to dissolve oxygen in the liquid culture medium;

[0061] S120: when the target bacteria grow to the logarithmic growth phase in the liquid culture medium, induce the target bacteria to express proteins that can bind to each other, so as to generate an engineered bacterial adhesion mixture with a network structure;

[0062] S130: use the engineered bacterial adhesion mixture to prepare a sensor sensitive layer.

[0063] Optionally, the method further includes the following steps:

[0064] Inject the engineered bacterial adhesion mixture into the catheter through a syringe, add wires at both ends of the catheter respectively, and then seal the two ends of the catheter with hot melt adhesive;

[0065] The two wires are connected to a power source and the resistance of the conduit when pressure is applied is tested.

[0066] Optionally, the method further comprises the steps of:

[0067] Venting gas from the conduit.

[0068] Optionally, the inoculating the target bacteria into the sterile culture medium comprises the steps of:

[0069] Inoculating Escherichia coli as the target bacteria into the sterile culture medium.

[0070] Optionally, the inducing the target bacteria to express the mutually combinable proteins when the target bacteria grow to the logarithmic growth phase in the liquid culture medium so that the engineered bacteria adhesion mixture with a network structure is generated comprises the steps of:

[0071] Inducing the target bacteria to express the mutually combinable antigen proteins and antibody proteins by genetic engineering when the target bacteria grow to the logarithmic growth phase in the liquid culture medium so that the engineered bacteria adhesion mixture with a network structure is generated after the antigen proteins combine with the antibody proteins.

[0072] The application is described below in the detailed description.

[0073] Reference Figure 2 The embodiment provides an example flowchart of a method for manufacturing a sensitive layer of a high-detection-upper-limit pressure sensor.

[0074] Specifically, the embodiment comprises the following technical solutions:

[0075] 1. Bacteria preparation:

[0076] (1) Bacteria activation and culture medium preparation: select appropriate bacteria strains, take them out from the preservation state for activation. In a sterile environment, inoculate the bacteria into the culture medium and place it in a suitable temperature (37°C) condition for 12-24 hours to restore the growth vitality.

[0077] (2) Bacteria amplification culture: take a small amount of bacteria from the activated bacteria and inoculate it into the liquid culture medium. Place the culture medium in a shaker to ensure that oxygen is fully dissolved in the culture medium, which is beneficial to the aerobic respiration and growth metabolism of bacteria. The culture temperature is maintained within the suitable growth range of bacteria. Take samples regularly to detect the growth of bacteria. The growth density of bacteria can be judged by measuring the turbidity (OD value) of the culture solution. When the bacteria grow to the logarithmic growth phase, the next step can be performed.

[0078] (3) Inducing expression: Through genetic engineering, we induce E. coli (this example is not limited to using this bacterium) to express two kinds of antigen proteins and antibody proteins that can bind to each other on the surface (this example is not limited to using this pair of proteins), and each bacterium only expresses one protein. These two proteins will connect together after contacting each other, so that each bacterium is connected and adhered to each other.

[0079] 2. Injecting bacteria into the catheter:

[0080] These connected and adhered bacteria are further used as monomer components to generate an engineered bacterial adhesion mixture with a network structure. This mixture forms a stable bacterial network using specific adhesion factors on the surface of the bacteria, and this structure has good mechanical properties. Subsequently, this mixture is carefully injected into a soft catheter using a syringe to ensure that the bacterial network is evenly distributed and maintains its structural integrity. During the injection process, it must be ensured that no air bubbles enter the tube, as the presence of air bubbles can affect the stability of the network structure and even break the effective connection of the bacteria, thereby reducing the performance of the mixture.

[0081] 3. Sealing the catheter to prevent leakage:

[0082] The two ends of the catheter are connected to the wires through precise connection methods to ensure the stability and reliability of signal transmission. In order to prevent any interference or leakage at the contact point of the wires and the catheter caused by external factors, hot melt adhesive is used for sealing treatment. The hot melt adhesive flows rapidly and fills the gaps at the connection site after heating, and forms a solid sealing layer after cooling, which not only effectively prevents the entry of air, moisture or other contaminants, but also maintains the stability of the bacterial mixture in the tube. This sealing process also increases the mechanical strength of the connection site of the catheter, ensuring that it is not easy to loosen or fall off during long-term use, thereby ensuring the long-term reliability and safety of the equipment. In addition, the use of hot melt adhesive makes the entire connection process simple and efficient, which helps to improve production efficiency.

[0083] 4. Connecting power supply, pressure detection:

[0084] The wires are connected to the power source through precise connections, forming a complete circuit system. When the user presses the bacteria-loaded conduit, the structure of the bacterial network inside the tube changes, causing a measurable change in resistance. Since the bacteria are connected through surface adhesion proteins, this change is closely related to the applied pressure, allowing the sensor to sensitively detect different intensities of pressure. As the pressure increases, the morphology and connection of the bacterial network adjust, causing a corresponding change in resistance, which can be monitored and fed back in real time through the circuit sensor. Through precise measurement of resistance changes, the sensor can achieve a high upper limit of detection for high-pressure environments, maintaining stable performance in complex or extreme conditions. This process not only accurately captures small pressure fluctuations but also makes the pressure sensor highly adaptable and reliable in various application scenarios, especially in situations requiring high sensitivity and a wide range of pressures.

[0085] 5. Pressure measurement effect:

[0086] When external pressure is applied, the pressure sensor of the engineered bacterial adhesion material responds to changes in external pressure, causing a change in resistance. This resistance change is a certain function of the applied pressure. These resistance signals can be monitored in real time through the circuit system and converted into voltage or other measurable output forms, ultimately representing a continuous pressure-resistance relationship curve, i.e., the R-P curve. Referring to Figure 3 By accurately analyzing the R-P curve, the resistance values corresponding to different pressures can be clearly determined, achieving high-precision pressure detection. This sensor performs excellently in terms of pressure range, accurately detecting high pressures close to 15 MPa, which makes it still have good responsiveness and stability in various extreme environments. The R-P curve provides an intuitive and reliable way to characterize the performance of the pressure sensor, allowing the sensor to be widely used in high-pressure detection fields, especially in industrial applications requiring accurate measurement and control.

[0087] In summary, the embodiment includes the following technical solutions:

[0088] 1. Bacteria used as the sensitive layer of the pressure sensor: This scheme uses bacteria as a living organism for the pressure sensor. Since bacteria can reproduce in large quantities, the material cost is low and the preparation process is simple, and it is biodegradable, with the characteristics of environmental friendliness.

[0089] 2. The pressure sensor has an ultra-high upper limit of detection: The bacteria-prepared pressure sensor has a high upper limit of detection. The unique properties of this material allow the sensor to maintain stable signal output in extremely high-pressure environments.

[0090] Compared with the prior art, the embodiment has the following significant advantages:

[0091] 1. Enhanced high-pressure detection capability:

[0092] Existing technology disadvantages: Traditional pressure sensors often have reduced sensitivity in extremely high-pressure environments and are easily disturbed by external factors, leading to unstable detection signals.

[0093] Advantages of this embodiment: The engineered bacterial adhesion material used in this embodiment has excellent high-pressure detection capability. This material can maintain stable signal output under high-pressure conditions, significantly improving the sensitivity and detection accuracy of the sensor. At the same time, the pressure resistance and reliability of the engineered bacterial adhesion material ensure the effectiveness of the sensor in extreme environments.

[0094] 2. Low material cost and simple preparation process:

[0095] Existing technology disadvantages: Traditional sensor materials require the purchase of expensive raw materials from specific suppliers, such as some special metals, semiconductors, etc. The preparation process of traditional sensors requires large and expensive production equipment. For example, semiconductor sensor manufacturing requires high-precision photolithography machines, vacuum coating equipment, etc.

[0096] Advantages of this embodiment: Bacteria can be mass-produced under specific culture conditions, with relatively low costs. The growth and metabolism of bacteria generally occur at normal temperature and pressure, without the need for special high-temperature, high-pressure, or extreme environmental conditions. This not only reduces energy consumption and equipment requirements, but also simplifies the process flow and reduces production costs.

[0097] 3. Biodegradable and environmentally friendly:

[0098] Existing technology disadvantages: Traditional plastic or metal materials generate a large amount of waste that is difficult to handle, increasing waste disposal costs and the risk of environmental pollution.

[0099] Advantages of this embodiment: Bacteria-made sensor materials are biodegradable, which is more in line with the concept of sustainable development in the context of increasingly stringent environmental requirements. Biodegradable bacterial sensors help reduce environmental impact and avoid additional costs due to environmental pollution.

[0100] In some alternative embodiments, the functions / operations described in the block diagrams can not occur in the order described in the operational illustrations. For example, two blocks shown in succession can in fact be executed substantially concurrently or the blocks can sometimes be executed in the reverse order, depending upon the functionality / operations involved. Also, although the flow diagrams present the embodiments of the application as a collection of steps occurring in a certain order, the present application is not so limited. Various embodiments of the present application can occur missing some of the operations, occurring in a different order than presented, or occurring concurrently. For example, the operations described in the flow diagrams can be performed in parallel, or the operations can be performed in an order different than that presented.

[0101] In the description of the present application, the terms "one embodiment", "some embodiments", "an example", "a specific example", or "some examples" are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present application. The illustrative appearances of the above terms in various places in the specification are not intended to exclude that the terms in other places mean the same or similar features, structures, materials, or characteristics. Furthermore, examples of the specific features, structures, materials, or characteristics are described in the specification, and each combination of features, structures, materials, or characteristics is also an example of the present application.

[0102] Although embodiments of the present application have been shown and described, it would be recognized by those of ordinary skill in the art that various changes, modifications, substitutions, and alterations can be made hereto without departing from the spirit and scope of the application. The scope of the present application is limited only by the claims and the equivalents thereof.

[0103] The above is a specific description of the preferred embodiments of the present application, but the present application is not limited to the described embodiments, and those skilled in the art can make various equivalent modifications or replacements without departing from the spirit of the present application, and these equivalent modifications or replacements are all included in the scope defined by the claims of the present application.

Claims

1. A method for manufacturing a sensitive layer of a high detection limit pressure sensor, characterized in that: The method comprises the following steps: The target bacteria are inoculated into a sterile culture medium and cultured at a set temperature for a set time to obtain activated target bacteria; inoculating the activated target bacteria into a liquid culture medium, and placing the liquid culture medium in a shaker so that oxygen is dissolved in the liquid culture medium; When the target bacteria grow to a logarithmic growth phase in the liquid culture medium, inducing the target bacteria to express proteins that can bind to each other, so as to generate an engineered bacterial adhesion mixture having a network structure; The engineered bacteria adhesion mixture is used to prepare a sensor sensitive layer.

2. The method for manufacturing a sensitive layer of a pressure sensor with a high detection limit according to claim 1, characterized in that: The method further comprises the following steps: Injecting the engineered bacterial adhesion mixture into a catheter through a syringe, adding wires to both ends of the catheter, and then sealing both ends of the catheter with hot melt adhesive; A power source was connected to the two wires, and the resistance of the catheter when pressure was applied was tested.

3. The method for manufacturing a sensitive layer of a pressure sensor with a high detection limit according to claim 2, wherein: The method further comprises the following steps: The gas in the conduit is vented.

4. The method for manufacturing a sensitive layer of a pressure sensor with a high detection limit according to claim 1, wherein: The method of inoculating the target bacteria into a sterile culture medium comprises the following steps: Escherichia coli is inoculated as the target bacterial species into the sterile culture medium.

5. The method for manufacturing a sensitive layer of a pressure sensor with a high detection limit according to any one of claims 1 to 4, characterized in that: When the target bacteria grow to the logarithmic growth phase in the liquid culture medium, inducing the target bacteria to express proteins that can bind to each other, so as to generate an engineered bacterial adhesion mixture with a network structure, comprising the following steps: When the target bacteria grow to the logarithmic growth phase in the liquid culture medium, the target bacteria are induced to express antigen proteins and antibody proteins that can bind to each other through genetic engineering modification, so that the antigen proteins and the antibody proteins bind to generate an engineered bacterial adhesion mixture with a network structure.

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