Method for detecting correlation between quality of radix tetrastigme and soil components

Abstract

The invention discloses a method for detecting correlation between radix tetrastigme quality and soil components, which comprises the following steps: respectively pretreating radix tetrastigme and planting soil under different planting conditions to obtain corresponding liquid to be detected; respectively detecting the content of chemical components in the to-be-detected liquid of the radix tetrastigme, and respectively detecting the content of essential elements and trace elements in the to-be-detected liquid of the planting soil; determining weights of corresponding items by adopting an entropy weight method according to the contents of the chemical components under the corresponding planting conditions; and evaluating the quality of the radix tetrastigme according to the weight of each item under the corresponding planting condition by adopting a superior and inferior solution distance method. The correlation degree between the quality of the radix tetrastigme and the soil components is determined through a grey correlation degree method, and when the correlation degree exceeds a preset threshold value, it is considered that the correlation between the quality of the radix tetrastigme and corresponding elements in the soil components is high. The method is high in detection accuracy, and the planting quality of the radix tetrastigme can be improved.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of analytical detection, and particularly relates to a detection method for the correlation between the quality of Radix Tetrastigmatis and soil components. BACKGROUND

[0002] Radix Tetrastigmatis is the tuber of Tetrastigma hemsleyanum Diels et Gilg in the grape family, and the quality of Radix Tetrastigmatis on the market is uneven, and the components of the soil for cultivation are closely related to the quality of traditional Chinese medicinal materials.

[0003] The total nitrogen content in the soil has different regulatory effects on the accumulation of effective components of different traditional Chinese medicinal materials, the total phosphorus in the soil is conducive to the accumulation of total flavones in traditional Chinese medicinal materials, in addition, the change of the total phosphorus content in the soil will affect the physical and chemical properties of the soil, and then indirectly affect the growth environment of traditional Chinese medicinal materials. The application of potassium fertilizer can improve the medicinal quality and yield of traditional Chinese medicinal materials, and promote the growth of rhizome medicinal materials such as Corydalis and Rhizoma Liriopis, in addition, the effective potassium content in the soil is closely related to the medicinal components of traditional Chinese medicinal materials. Appropriate ammonium nitrogen supply can promote the growth of traditional Chinese medicinal materials, ammonium nitrogen indirectly promotes the accumulation of medicinal components by affecting the activity of plant metabolic enzymes, and excessive application may cause soil acidification and inhibit nitrification, thereby affecting the absorption of nitrate nitrogen by plants. In summary, reasonable fertilization and soil management are the key measures to improve the quality of traditional Chinese medicinal materials.

[0004] Geographical location and soil type and other factors can significantly affect the solubility of copper and iron in the soil and the absorption capacity of plants for these important trace elements, and the accumulation of secondary metabolites of traditional Chinese medicinal materials is closely related to soil nutrients. By detecting and determining the quality of medicinal materials and the content of essential elements in the soil under different proportions of substrates for Radix Tetrastigmatis, the influence of each element in the planting substrate on the quality of Radix Tetrastigmatis is considered, and the correlation between the two is determined, which is of great significance to avoid uneven quality of cultivated Radix Tetrastigmatis. SUMMARY

[0005] The purpose of the present application is to solve the above problems, and a detection method for the correlation between the quality of Radix Tetrastigmatis and soil components is provided, which has high detection accuracy and helps to improve the planting quality of Radix Tetrastigmatis.

[0006] To achieve the above purpose, the technical solution adopted by the present application is as follows:

[0007] The detection method for the correlation between the quality of Radix Tetrastigmatis and soil components provided by the present application comprises the following steps:

[0008] S1, pretreat Radix Tetrastigmatis and planting soil under different planting conditions respectively to obtain corresponding test solutions;

[0009] S2, the test solution of three-leaf green is subjected to content detection of chemical components, and the test solution of the planting soil is subjected to content detection of essential elements and trace elements;

[0010] S3, the weight of the corresponding item is determined according to the content of the chemical components under the corresponding planting condition by using the entropy weight method;

[0011] S4, the quality of three-leaf green is evaluated according to the weight of each item under the corresponding planting condition by using the distance method of superior and inferior solutions;

[0012] S5, the yield of three-leaf green under the planting condition with the best quality of three-leaf green and the content of the top K items with the highest weight in the chemical components are taken as a mother sequence, the content of essential elements and trace elements in the corresponding planting soil are taken as a comparison sequence, the correlation degree between the quality of three-leaf green and the soil components is determined by using the grey correlation degree method, and when the correlation degree exceeds a preset threshold, it is considered that the quality of three-leaf green has high correlation with the corresponding elements in the soil components.

[0013] Preferably, the chemical components include extract, total flavonoids, total polysaccharides, catechin, epicatechin, syringin, astragalin, kaempferol, kaempferol-3-O-β-D- apiosyl-(1→2)-β-D-glucosyl-7-O-α-L-rhamnoside and kaempferol-3-O-β-D-glucosyl-7-O-α-L-rhamnoside, the essential elements include total nitrogen, total phosphorus, available potassium and ammonium nitrogen, and the trace elements include iron and copper.

[0014] Preferably, the content detection of extract adopts a reflux extraction method, the content detection of total flavonoids, total polysaccharides, total nitrogen, total phosphorus, available potassium, ammonium nitrogen, iron and copper adopts an ultraviolet-visible spectrophotometer method, and the content detection of catechin, epicatechin, syringin, astragalin, kaempferol, kaempferol-3-O-β-D-apiosyl-(1→2)-β-D-glucosyl-7-O-α-L-rhamnoside and kaempferol-3-O-β-D-glucosyl-7-O-α-L-rhamnoside adopts a high performance liquid chromatography method.

[0015] Preferably, the content detection of extract is specifically as follows:

[0016] 10 ml-30 ml of the test solution of extract is taken, placed in an evaporating dish dried to constant weight, evaporated to dryness on a water bath at 90°C-100°C, dried in an electric heating air drying oven at 100°C-110°C for 3 hours, then cooled in a desiccator for 0.5 h-1.0 h, and weighed to obtain the content of extract in the test solution of extract, unit %, satisfying , M J is the content of extract in the test solution of extract, C J is the weight of the dried product, β J is the first dilution coefficient, GJ W is the weight of the test sample as the extract J W is the weight of the test sample as the extract

[0017] The content of total flavonoids is detected as follows:

[0018] 5 mg-15 mg of rutin reference substance is weighed, dissolved by ultrasonic after adding 5 ml-8 ml of methanol with a concentration of 70%-100%, cooled to room temperature, and then added with methanol with a concentration of 70%-100% to the 10 ml mark of the volumetric flask, shaken well, and a rutin reference substance solution is obtained. 0 ml, 0.1 ml, 0.2 ml, 0.4 ml, 0.7 ml, and 1.0 ml of the rutin reference substance solution are measured respectively, and water is added to 1 ml. The rutin reference substance solution after water addition and 0.5 ml-2 ml of total flavonoids to be tested are respectively placed in different volumetric flasks. 200 μL-500 μL of NaNO2 with a concentration of 5% is sequentially added in each volumetric flask, shaken well, and allowed to react for 5 min-10 min, 200 μL-500 μL of Al(NO3)3 with a concentration of 10% is sequentially added in each volumetric flask, shaken well, and allowed to react for 5 min-10 min, 2 ml-5 ml of NaOH with a concentration of 4% is sequentially added in each volumetric flask, shaken well, and allowed to react for 20 min-40 min, water is added to the 10 ml mark of the volumetric flask, shaken well, and allowed to stand for 10 min-20 min. A reagent formed by the rutin reference substance solution is obtained. The absorbance is detected by an ultraviolet-visible spectrophotometer. A linear regression equation of total flavonoids is formed according to the absorbance and concentration of different rutin reference substance solutions. The concentration of total flavonoids in the total flavonoids to be tested is calculated by substituting the absorbance of the total flavonoids to be tested into the linear regression equation of total flavonoids, and then multiplied by the second dilution coefficient. The content of total flavonoids in the total flavonoids to be tested is obtained. The average of the content of total flavonoids in the total flavonoids to be tested under the corresponding planting conditions is taken as the content of total flavonoids under the current planting conditions. The wavelength of the ultraviolet-visible spectrophotometer is set to 490 nm-520 nm.

[0019] The content of total polysaccharides is detected as follows:

[0020] Respectively, 0 μL, 25 μL, 50 μL, 100 μL, 200 μL, 400 μL glucose control and corresponding water make up to 0.4 ml-1 ml, the water make up after glucose control and 0.4 ml-1 ml total polysaccharide to be tested are respectively put into different glass test tubes, 1 ml-2 ml 5% concentration phenol solution and 3 ml-7 ml 90%-98% concentration concentrated sulfuric acid are added into each glass test tube in turn, mixed and reacted in boiling water bath for 30 min-60 min, cooled to room temperature after standing, 1.5 ml-2 ml supernatant is taken and added into quartz colorimetric cell, the absorbance is detected by UV-visible spectrophotometer, the linear regression equation of total polysaccharide is formed according to the absorbance and concentration of different glucose control, then the absorbance of total polysaccharide to be tested is substituted into the linear regression equation of total polysaccharide, the concentration of total polysaccharide in total polysaccharide to be tested is calculated, multiplied by the third dilution coefficient, which is the content of total polysaccharide in total polysaccharide to be tested, and the average of the content of total polysaccharide in all total polysaccharide to be tested under corresponding planting conditions is taken as the content of total polysaccharide under current planting conditions, and the wavelength of UV-visible spectrophotometer is set to 490 nm-520 nm;

[0021] The content of kaempferol-3-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside and kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside is detected as follows:

[0022] The reference substance kaempferol-3-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside and kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside are weighed in a volumetric flask to prepare a first mixed reference solution with the concentration of kaempferol-3-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside being 10 μg / ml-30 μg / ml and the concentration of kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside being 5 μg / ml-20 μg / ml; the first mixed reference solution and the test solution of kaempferol-3-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside and kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside are isocratically eluted by using a reversed-phase ODS column, the mobile phase A is acetonitrile, the mobile phase B is 0.085% phosphoric acid water, and acetonitrile:0.085% phosphoric acid water = 12:88, the detection wavelength is 348 nm, the flow rate is 1.0 ml / min, and the column temperature is 25-35°C; the content of kaempferol-3-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside and the content of kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside in the corresponding test solution are calculated according to the isocratic elution results, and the average of the content of kaempferol-3-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside and the average of the content of kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside in all corresponding test solutions under the corresponding planting conditions are respectively taken as the content of kaempferol-3-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside and the content of kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside under the current planting conditions.

[0023] The contents of catechin, epicatechin, tricin, astragalin, and kaempferol are detected as follows:

[0024] Take 1 mg-2 mg of control catechin, epicatechin, Melissa glycoside, Chinese milk vetch glycoside, kaempferol, respectively, into a volumetric flask, add 4 ml-8 ml of methanol with purity greater than or equal to 95%, ultrasonic dissolve for 15 min-60 min, and then dilute to 5 ml-10 ml; take 200 μl-2000 μl of each to mix and prepare a second mixed control solution, so that the final concentrations of catechin, epicatechin, Melissa glycoside, Chinese milk vetch glycoside and kaempferol standards are 15.9 μg / ml, 11.3 μg / ml, 7.2 μg / ml, 11.2 μg / ml and 2.0 μg / ml, respectively; use a C18 chromatographic column to gradient elute the second mixed control solution and catechin, epicatechin, Melissa glycoside, Chinese milk vetch glycoside and kaempferol test solution, mobile phase A: acetonitrile, mobile phase B: 0.085% phosphoric acid, acetonitrile elution gradient: 0 min-20 min, 10%-15%; 20 min-40 min, 15%-85%; 40 min-55 min, 35%-100%; detection wavelength 230 nm-360 nm, flow rate 1.0 ml / min, column temperature 25℃-35℃; calculate the content of catechin, epicatechin, Melissa glycoside, Chinese milk vetch glycoside and kaempferol in the corresponding test solution according to the isocratic elution results, and take the average of the catechin content, epicatechin content, Melissa glycoside content, Chinese milk vetch glycoside content and kaempferol content in all corresponding test solutions under the corresponding planting conditions as the catechin content, epicatechin content, Melissa glycoside content, Chinese milk vetch glycoside content and kaempferol content under the current planting conditions, respectively;

[0025] The content of total nitrogen and ammonium nitrogen is detected as follows:

[0026] Take 0.5-1 ml of nitrogen standard solution, total nitrogen to be tested solution and ammonium nitrogen to be tested solution respectively, add 50-100 μl of mixed accelerator and shake, then add 50-100 μl of indicator, shake, stand for 15-60 min, add water to make up to 2-5 ml, detect the absorbance by UV-visible spectrophotometer, calculate the concentration of total nitrogen to be tested solution according to the absorbance of total nitrogen to be tested solution, the absorbance of nitrogen standard solution and the concentration of nitrogen standard solution, which is the content of total nitrogen in total nitrogen to be tested solution, and calculate the concentration of ammonium nitrogen to be tested solution according to the absorbance of ammonium nitrogen to be tested solution, the absorbance of nitrogen standard solution and the concentration of nitrogen standard solution, which is the content of ammonium nitrogen in ammonium nitrogen to be tested solution, and take the average of the content of total nitrogen in all total nitrogen to be tested solution and the average of the content of ammonium nitrogen in all ammonium nitrogen to be tested solution under corresponding planting conditions as the content of total nitrogen and the content of ammonium nitrogen under current planting conditions respectively, set the wavelength of UV-visible spectrophotometer to 410-430 nm, the mixed accelerator is potassium sulfate: copper sulfate: selenium = 100: 10: 1 dissolved in 10 times volume of water, and the indicator is methyl red: bromo-chloro-indolyl blue = 1:5 dissolved in 10 times ethanol;

[0027] The content of total phosphorus is detected as follows:

[0028] Take 0.5-1 ml of phosphorus standard solution and total phosphorus to be tested solution respectively, add 50-100 μl of color developing agent and shake, then add 50-100 μl of 0.5% antimony potassium tartrate solution and shake, stand for 5-10 min, add water to make up to 2-5 ml, detect the absorbance by UV-visible spectrophotometer, calculate the concentration of total phosphorus to be tested solution according to the absorbance of total phosphorus to be tested solution, the absorbance of phosphorus standard solution and the concentration of phosphorus standard solution, which is the content of total phosphorus in total phosphorus to be tested solution, and take the average of the content of total phosphorus in all total phosphorus to be tested solution under corresponding planting conditions as the content of total phosphorus under current planting conditions, set the wavelength of UV-visible spectrophotometer to 700 nm, and the color developing agent is 10 g of ammonium molybdate dissolved in 1 L of water and then 126 ml of 90-98% concentrated sulfuric acid is added;

[0029] The content of effective potassium is detected as follows:

[0030] Respectively, potassium standard solution and effective potassium to be measured 0.5 ml-1 ml, 50 μl-100 μl concentration of 0.5 M sodium sulfate solution is added, shake, then add 50 μl-100 μl sodium tetraphenylborate solution, shake, 5 min-10 min after the addition of water to 2 ml-5 ml, the ultraviolet-visible spectrophotometer detection absorbance, according to the effective potassium to be measured absorbance, potassium standard solution and the concentration of potassium standard solution to calculate the concentration of effective potassium to be measured, namely the content of effective potassium in the effective potassium to be measured, and the corresponding planting conditions under the mean of all effective potassium to be measured in the content of effective potassium as the current planting conditions under the content of effective potassium, the wavelength of ultraviolet-visible spectrophotometer is set to 420 nm;

[0031] Iron content detection, as follows:

[0032] Respectively, iron standard solution and iron to be measured 50 μl-100 μl, sequentially add hydroxylamine hydrochloride solution 50 μl-100 μl, phenanthroline 50 μl-200 μl and acetic acid-sodium acetate buffer 100 μl-500 μl for color development, shake, 10 min-30 min after the addition of ultraviolet-visible spectrophotometer detection absorbance, according to the iron to be measured absorbance, iron standard solution and the concentration of iron standard solution to calculate the concentration of iron to be measured, namely the content of iron in the iron to be measured, and the corresponding planting conditions under the mean of all iron to be measured in the content of iron as the current planting conditions under the content of iron, the wavelength of ultraviolet-visible spectrophotometer is set to 510 nm;

[0033] Copper content detection, as follows:

[0034] Respectively, copper standard solution and copper to be measured 50 μl-100 μl, sequentially add 0.1 mol / l-0.5 mol / l concentration of ammonium citrate solution 500 μl, 0.05 mol / L-0.1 mol / L concentration of EDTA disodium salt solution 100 μl-500 μl and carbon tetrachloride 50 μl-200 μl shake, then add sodium diethyldithiocarbamate 0.02 g-0.05 g shake, 10 min-30 min after the addition of ultraviolet-visible spectrophotometer detection absorbance, according to the copper to be measured absorbance, copper standard solution and the concentration of copper standard solution to calculate the concentration of copper to be measured, namely the content of copper in the copper to be measured, and the corresponding planting conditions under the mean of all copper to be measured in the content of copper as the current planting conditions under the content of copper, the wavelength of ultraviolet-visible spectrophotometer is set to 510 nm.

[0035] Preferably, each linear regression equation is formed after drawing a standard curve with absorbance as the ordinate and the concentration of the corresponding item as the abscissa, and satisfies wherein x is absorbance, a and b are both constants, and y is the concentration of the corresponding item.

[0036] Preferably, before the planting soil is pretreated, the following operation is further performed:

[0037] N planting bags of Radix Aristolochiae Contortae are randomly taken from each test area for digging, and the following operation is performed on each planting bag during digging: a ring cut opening is made at a distance of 10 cm from the ground of the planting bag, and M sampling points are set at a diameter of 5 cm outward from the center of the ring cut opening, the crop roots, insects, and stones in the planting soil at each sampling point are removed, dried, and crushed, and then passed through a 1 mm-2 mm mesh sieve to screen out soil samples, which are packaged, stored, and labeled;

[0038] Before the Radix Aristolochiae Contortae is pretreated, the following operation is further performed:

[0039] The tuberous roots of Radix Aristolochiae Contortae in the corresponding planting bag are picked, washed, dried, and cut into 2 mm-4 mm thick slices, which are dried at 55°C, weighed, crushed, and passed through a 50 mesh sieve, the coarse powder that cannot pass through the 50 mesh sieve is mixed with the fine powder that passes through the 50 mesh sieve, and the proportion of fine powder in the mixed coarse powder and fine powder exceeds 90%, and the mixed coarse powder and fine powder are recorded as dry powder.

[0040] Preferably, the pretreatment is as follows:

[0041] 1) For extract detection:

[0042] 2 g-4 g of dry powder is weighed into a flat-bottom extraction bottle, 50 ml-100 ml of ethanol with a concentration of 70% is added, the bottle is tightly sealed and weighed, after standing for 1 h, the stopper is removed and a reflux condenser is connected, heating is performed until boiling and maintained for 1 h, after cooling to room temperature, the flat-bottom extraction bottle is removed, tightly sealed and weighed again, the lost weight is made up with water, and after shaking and filtering through a dry filter, a test solution of the extract is obtained;

[0043] 2) For total flavonoids, catechin, epicatechin, syringin, astragalin, kaempferol, kaempferol-3-O-β-D- apiofuranosyl-(1→2)-β-D-glucopyranosyl-7-O-α-L-rhamnopyranoside, and kaempferol-3-O-β-D-glucopyranosyl-7-O-α-L-rhamnopyranoside detection:

[0044] Take 1 g~2 g of dry powder into a flat-bottom extraction bottle, add 25 times-50 times volume of 60%-90% methanol, weigh, reflux extract at 80℃-95℃ for 1 h-2 h, cool to room temperature, add corresponding concentration of methanol to make up, filter the initial test solution, which is the test solution of total flavonoids; take 20 ml-40 ml of the initial test solution of the filtrate into an evaporating dish, concentrate to 10 ml-25 ml at 80℃-100℃ water bath, obtain the test solution of catechin, epicatechin, prunus triloba glycoside, astragalus glycoside, kaempferol, kaempferol-3-O-β-D-quercetin-(1→2)-β-D-glucoside-7-O-α-L-rhamnoside and kaempferol-3-O-β-D-glucoside-7-O-α-L-rhamnoside;

[0045] 3) for total polysaccharide detection:

[0046] Take 1 g-2 g of dry powder into a flat-bottom extraction bottle, add 50 ml-100 ml of deionized water, weigh, shake well and stand for 0.5 h-1.0 h, remove the plug and connect the reflux condenser, extract at 90℃-100℃ for 1.5 h-3.0 h, cool to room temperature, remove the flat-bottom extraction bottle, weigh again, add water to make up the weight loss, shake well, centrifuge at 3000 rpm-4500 rpm for 5 min-15 min, take 25 ml-50 ml of supernatant, add 1%-5% of α-amylase, shake at 35℃-50℃ for 10 min-30 min, take out, centrifuge at 3000 rpm-4500 rpm for 5 min-15 min, take 2 ml-10 ml of supernatant, add 3 times-5 times of anhydrous ethanol, shake well, refrigerate at 0℃-4℃ for more than 10 hours, centrifuge at 3000 rpm-4500 rpm for 5 min-15 min, discard the supernatant, add 5 ml-25 ml of deionized water and mix well to dissolve, add 1 / 3 times-1 / 5 times volume of chloroform-n-butanol mixed solution with chloroform:n-butanol=4:1, shake to remove protein for 1 h-2 h, the supernatant after removing protein is the test solution of total polysaccharide;

[0047] 4) for total nitrogen and total phosphorus detection:

[0048] Take 1 g-2 g of soil sample into a conical flask, add 0.5 ml-2 ml of pure water to form a wet sample, then add 4 ml-8 ml of concentrated sulfuric acid with a concentration of 90%-98% and 0.5 ml-2 ml of hydrogen peroxide in turn, cover the bottle mouth with a bent funnel for digestion, stop when white smoke appears, cool to room temperature, then transfer to a volumetric flask, dilute to 100 ml with pure water, shake well and stand for 10 min-20 min, take 20 ml-45 ml of the upper solution into a volumetric flask, add 2 ml-5 ml of 50% sodium hydroxide solution, dilute to 100 ml with pure water and shake well to obtain the test solution for total nitrogen and total phosphorus;

[0049] 5) for effective potassium detection:

[0050] Take 4 g-8 g of soil sample into an extraction flask, add 20 ml-40 ml of water and 1 g-2 g of ammonium acetate powder, shake for 10 min-20 min, then filter to obtain the test solution for effective potassium;

[0051] 6) for ammonium nitrogen detection:

[0052] Take 4 g-8 g of soil sample into an extraction flask, add 20 ml-40 ml of water and 1 g-2 g of potassium chloride powder, shake for 10 min-20 min, then filter to obtain the test solution for ammonium nitrogen;

[0053] 7) for iron and copper detection:

[0054] Take 5 g-10 g of soil sample into a conical flask, add 10 ml-20 ml of diethylamine tris(butyl acid) ethylenediamine pentaacetate extractant, shake for 0.5 h-2.0 h, then filter to obtain the test solution for iron and copper, the diethylamine tris(butyl acid) ethylenediamine pentaacetate extractant contains 0.005 mol / L of diethylene triamine pentaacetate, 0.01 mol / L of calcium chloride, and 0.1 mol / L of triethanolamine.

[0055] Preferably, the quality of Radix Caulis Tetrastigae under corresponding planting conditions is evaluated by using the ideal solution distance method according to the weight of each item, i.e. taking the yield of Radix Caulis Tetrastigae under corresponding planting conditions and the content of each item in the chemical composition as positive indicators, obtaining the optimal matrix vector and the worst matrix vector according to the weight of each item in the chemical composition, then calculating the comprehensive degree score index S after sorting according to the positive ideal solution distance D+ and the negative ideal solution distance D- of the corresponding planting conditions, the higher the value of the comprehensive degree score index S, the better the quality of Radix Caulis Tetrastigae under the corresponding planting conditions.

[0056] Preferably, the different planting conditions include seven ash treatment groups, which are sequentially recorded as group A, group B, group C, group D, group E, group F and group G, wherein the ash addition amount of group A is 0 kg and serves as a control group, the ash-based fertilizer of group B is added by 1 kg, the ash-based fertilizer of group C is added by 2 kg, the ash-based topdressing of group D is added by 1 kg, the ash-based topdressing of group E is added by 2 kg, the ash-based fertilizer of group F is added by 0.5 kg and the topdressing is added by 0.5 kg, and the ash-based fertilizer of group G is added by 1 kg and the topdressing is added by 1 kg.

[0057] Compared with the prior art, the method has the following beneficial effects:

[0058] The method can provide accurate basis for the planting mode and quality control of the Radix Caulis Tetrastigae by detecting the chemical components of the Radix Caulis Tetrastigae and the contents of the essential elements and trace elements in the planting soil and performing correlation analysis, and is beneficial to improving the quality of the Radix Caulis Tetrastigae. Specifically, the Radix Caulis Tetrastigae and the planting soil under different planting conditions are pretreated to obtain corresponding test solutions, the contents of the chemical components of the Radix Caulis Tetrastigae are detected, the contents of the essential elements and trace elements in the planting soil are detected, the weight of each item under the corresponding planting condition is determined by using the entropy weight method, the quality of the Radix Caulis Tetrastigae is evaluated by using the ideal and poor solution distance method according to the weight of each item under the corresponding planting condition, the correlation degree between the quality of the Radix Caulis Tetrastigae and the soil components under the planting condition with the best quality of the Radix Caulis Tetrastigae is analyzed by using the grey correlation degree method, the correlation degree between the quality of the Radix Caulis Tetrastigae and the corresponding element in the soil components is considered to be high when the correlation degree exceeds a preset threshold, especially the content detection method of the total flavonoids and total polysaccharides of the Radix Caulis Tetrastigae can greatly reduce the operation error of the detector and the influencing factors of subsequent detection under the premise of ensuring the accuracy of the results, and the content detection method of kaempferol-3-O-beta-D-quercetin-(1→2)-beta-D-glucoside-7-O-alpha-L-rhamnoside and kaempferol-3-O-beta-D-glucoside-7-O-alpha-L-rhamnoside with high content in the Radix Caulis Tetrastigae is established, and the stability and accuracy are high. BRIEF DESCRIPTION OF DRAWINGS

[0059] Figure 1 The flow chart of the detection method of the correlation between the quality of the Radix Caulis Tetrastigae and the soil components is shown in the figure;

[0060] Figure 2 The liquid chromatogram of the mixed control solution and the test solution of F1 and F2 is shown in the figure;

[0061] Figure 3 The content detection result graph of the chemical components of the Radix Caulis Tetrastigae is shown in the figure;

[0062] Figure 4 The content detection result graph of the essential elements and trace elements in the planting soil is shown in the figure. DETAILED DESCRIPTION

[0063] The technical solutions in the embodiments of the present application will be described clearly and completely in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all the other embodiments obtained by those of ordinary skill in the art without creative work fall within the scope of the present application.

[0064] It should be noted that, unless otherwise defined, all the technical and scientific terms used herein have the same meanings as commonly understood by those skilled in the art of the present application. The terms used in the specification of the present application are only for the purpose of describing the specific embodiments and are not intended to limit the present application.

[0065] As shown in Figures 1-4 A method for detecting the correlation between the quality of Radix Ternatae and soil components, comprising the following steps:

[0066] Different proportions and different ways of adding wood ash in the planting soil, setting group A (control group, adding wood ash 0 kg), group B (adding wood ash base fertilizer 1 kg), group C (adding wood ash base fertilizer 2 kg), group D (adding wood ash topdressing 1 kg), group E (adding wood ash topdressing 2 kg), group F (adding wood ash base fertilizer 0.5 kg + topdressing 0.5 kg), group G (adding wood ash base fertilizer 1 kg + topdressing 1 kg), that is, including 7 wood ash treatment groups (including the control group, that is, group A), under the conditions of conventional planting and irrigation in the base, the different wood ash addition proportions and addition methods (that is, different planting conditions) are investigated. The selection of each group above is preferably combined with the economic cost and the cultivation experience of Radix Ternatae in the base for many years. Specifically, in the present embodiment, after 6 months of seedling raising, when planting Radix Ternatae, Radix Ternatae is divided into the above 7 wood ash treatment groups; after 3 years of planting (6 months of seedling raising + 2 years and 6 months), the Radix Ternatae is dug in winter (November-February of next year, November 30 in the present embodiment).

[0067] The threeleaf ginseng and planting soil used in the examples were from Quzhou City, Zhejiang Province, China. N = 3 planting bags of threeleaf ginseng were randomly selected from each test area (i.e., each planting condition) for digging. When digging, M = 3 sampling points were fixed at a distance of 10 cm from the ground and a diameter of 5 cm from the center of the planting bag. The crop roots, insects, stones, and other debris in the planting soil were removed with tweezers and placed in a cool and ventilated room for air drying. The air-dried soil samples were crushed with a wooden stick and sieved through a 1 mm-2 mm sieve. Three soil samples (corresponding to three planting bags) were obtained from each test area, i.e., the screened soil samples were packaged and labeled for future use. The threeleaf ginseng tubers from the corresponding planting bags were washed, dried, and cut into 2 mm-4 mm thick slices. The slices were dried at 55°C, weighed, and then pulverized through a 50 mesh sieve using a high-speed pulverizer. The coarse powder that could not pass through the 50 mesh sieve was mixed with the fine powder that passed through the 50 mesh sieve to ensure uniformity of the dry powder. The proportion of fine powder in the mixed coarse and fine powder was 90%. Similarly, three dry powder samples were obtained from each test area.

[0068] The threeleaf ginseng and planting soil under different planting conditions were pretreated to obtain the corresponding test solutions. The pretreatment was as follows:

[0069] 1) For extract detection:

[0070] 2 g of the test sample (dry powder) was weighed (precisely to 0.0001 g) into a flat-bottom extraction bottle, 50 ml of 70% ethanol was added, and the bottle was tightly sealed and weighed. After standing for 1 h, the stopper was removed and a reflux condenser was connected. The solution was heated to boiling and maintained for 1 h. After cooling to room temperature, the flat-bottom extraction bottle was removed, tightly sealed, and reweighed. Water was added to make up for the weight loss, and the solution was shaken and filtered through a dry filter to obtain the filtrate, which was the test solution for the extract.

[0071] 2) For total flavonoids, catechin, epicatechin, syringin, astragalin, kaempferol, kaempferol-3-O-β-D-apiosyl-(1→2)-β-D-glucosyl-7-O-α-L-rhamnoside, and kaempferol-3-O-β-D-glucosyl-7-O-α-L-rhamnoside detection:

[0072] Take 1 g of dry powder (accurately weighed to 0.0001 g) into a 100 ml flat-bottom extraction bottle, add 25 times the volume of 70% methanol, weigh, extract at 85°C for 1 h, cool to room temperature, add 70% methanol to make up, filter the initial test solution, which is the total flavonoids test solution; take 25 ml of the filtrate of the initial test solution into an evaporating dish and concentrate on a 100°C water bath, concentrate the solution to 10 ml to obtain the catechin, epicatechin, prunus triloba glycoside, astragalus glycoside, kaempferol, kaempferol-3-O-β-D- apigenin- (1→2) -β-D-glucoside-7-O-α-L-rhamnoside and kaempferol-3-O-β-D-glucoside-7-O-α-L-rhamnoside test solution.

[0073] 3) When detecting total polysaccharides:

[0074] Take 1 g of dry powder (accurately weighed to 0.0001 g) into a 100 ml flat-bottom extraction bottle, add 50 ml of deionized water, weigh, shake well and stand for 0.5 h, remove the plug and connect the reflux condenser, extract at 95°C for 2 h, cool to room temperature, remove the flat-bottom extraction bottle, weigh again, make up the weight loss with water, shake well and centrifuge at 3000 rpm for 10 min, take 25 ml of the supernatant and add 1% α-amylase, hydrolyze at 50°C for 10 min, remove, centrifuge at 3000 rpm for 10 min, take 2 ml of the supernatant, add 3 times the volume of anhydrous ethanol, shake well, refrigerate at 4°C for 10 h, centrifuge at 3000 rpm for 10 min, discard the supernatant, dissolve the precipitate in 5 ml of deionized water, add 1 / 4 times the volume of chloroform-n-butanol mixed solution (chloroform-n-butanol = 4:1) to remove protein for 1 h, and the supernatant after removing protein is the total polysaccharide test solution.

[0075] 4) When detecting total nitrogen and total phosphorus:

[0076] Take 1 g of soil sample (accurately weighed to 0.0001 g) into a 100 ml conical flask, add 1 ml of pure water to form a wet sample, then add 5 ml of 98% concentrated sulfuric acid and 1 ml of hydrogen peroxide in turn, cover the mouth of the conical flask with a bent funnel for digestion, stop when white smoke appears, cool naturally at room temperature, transfer to a volumetric flask, dilute to 100 ml with pure water, shake well and stand for 10 min, take 25 ml of the upper solution into a 100 ml volumetric flask, add 2 ml of 50% sodium hydroxide solution, dilute to 100 ml with pure water and shake well to obtain the total nitrogen and total phosphorus test solution.

[0077] 5) When detecting effective potassium:

[0078] Take 4 g of soil sample (precisely weighed to 0.0001 g) in the extraction bottle, add 20 ml of water and 1 g of ammonium acetate powder, shake for 15 min, then filter to obtain the test solution of available potassium.

[0079] 6) When detecting ammonium nitrogen:

[0080] Take 4 g of soil sample (precisely weighed to 0.0001 g) in the extraction bottle, add 20 ml of water and 1 g of potassium chloride powder, shake for 15 min, then filter to obtain the test solution of ammonium nitrogen.

[0081] 7) When detecting iron and copper:

[0082] Take 5 g of soil sample (precisely weighed to 0.0001 g) in a 50 ml conical flask, add 10 ml of diethylamine tris(butyl acid) ethylenediamine pentacetic acid extractant, shake for 1 h, then filter to obtain the test solution of iron and copper. The diethylamine tris(butyl acid) ethylenediamine pentacetic acid extractant contains 0.005 mol / L of diethylene triamine pentaacetic acid (DTPA), 0.01 mol / L of calcium chloride (CaCl2), and 0.1 mol / L of triethanolamine.

[0083] The test solution of San Ye Qing was subjected to content detection of chemical components and content detection of essential elements and trace elements in the planting soil, respectively. The chemical components included 10 items such as extract, total flavonoids, total polysaccharides, catechin, epicatechin, syringin, astragalin, kaempferol, kaempferol-3-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranosyl-7-O-α-L-rhamnopyranoside (F1), and kaempferol-3-O-β-D-glucopyranosyl-7-O-α-L-rhamnopyranoside (F2). The essential elements included total nitrogen, total phosphorus, available potassium, and ammonium nitrogen. The trace elements included iron and copper. Among them, the content detection of extract was performed by alcohol-soluble extract detection using reflux extraction method. The content detection of total flavonoids, total polysaccharides, total nitrogen, total phosphorus, available potassium, ammonium nitrogen, iron, and copper was performed by ultraviolet-visible spectrophotometry. The content detection of catechin, epicatechin, syringin, astragalin, kaempferol, kaempferol-3-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranosyl-7-O-α-L-rhamnopyranoside, and kaempferol-3-O-β-D-glucopyranosyl-7-O-α-L-rhamnopyranoside was performed by high performance liquid chromatography (HPLC).

[0084] The content detection of extract was as follows:

[0085] The measured solution of the extract 25 ml was taken and placed in a dry evaporating dish to constant weight. After evaporating in a water bath at 100°C, it was dried in an electric heating air drying oven at 105°C for 3 hours, then cooled in a desiccator for 0.5 h. The weight of the cooled dry product was measured to obtain the content of the extract in the measured solution of the extract, unit %, which met , M J is the content of the extract in the measured solution of the extract, C J is the weight of the dry product, β J is the first dilution coefficient, G J is the weight of the test sample of the extract, W J is the moisture content of the test sample of the extract, unit %, wherein the test sample of the extract here is the dry powder used for pretreatment for the detection of the extract. The moisture content of the test sample is obtained by conventional techniques for those skilled in the art, such as evaporation drying by a similar scheme. In this embodiment, the coefficient is 2, i.e. half of the measured solution of the extract is taken for detection. The average of the content of the extract in the measured solution of all the extracts under the corresponding planting conditions is taken as the content of the extract under the current planting conditions. In this embodiment, there are N=3 planting bags of samples under each planting condition, and the average of the content of the extract of the three samples is taken as the content of the extract under the current planting condition. The same applies to others. The first dilution coefficient is the ratio of the volume of the measured solution of the extract obtained by pretreatment to the volume of the measured solution of the extract taken for content detection. In this embodiment, the measured solution of the extract taken for content detection is 25 ml, while the measured solution of the extract obtained by pretreatment is 50 ml, so the first dilution coefficient is 2 times. The specific adjustment is made according to the actual demand. The other dilution coefficients are obtained in the same way.

[0086] The content of total flavonoids was detected as follows:

[0087] Take 10 mg of rutin reference substance, add 8 ml of 70% methanol, and then ultrasonic dissolve. Cool to room temperature, then add 70% methanol to the 10 ml mark of the volumetric flask, shake well, and obtain the rutin reference substance solution. Respectively, take 0 ml, 0.1 ml, 0.2 ml, 0.4 ml, 0.7 ml, and 1.0 ml of the rutin reference substance solution, and add water to make up to 1 ml. Put the water added rutin reference substance solution and 1 ml of total flavonoids to be tested into different volumetric flasks. Respectively, add 500 μL of 5% NaNO2 in each volumetric flask, shake well, and stand for 6 min. Add 500 μL of 10% Al(NO3)3, shake well, and stand for 6 min. Add 4 ml of 4% NaOH, shake well, and react for 30 min. Add water to the 10 ml mark of the volumetric flask, shake well, and stand for 15 min. Obtain the reagent formed by the rutin reference substance. Calibrate the ultraviolet-visible spectrophotometer with the reagent formed by the rutin reference substance of 0 ml of rutin standard solution. Respectively, detect the linear regression equation of the absorbance formed by total flavonoids using the ultraviolet-visible spectrophotometer. Obtain the concentration of total flavonoids in the total flavonoids to be tested according to the linear regression equation of total flavonoids, multiply by the second dilution coefficient, and the content of total flavonoids in the total flavonoids to be tested is obtained. The average of the contents of total flavonoids in all total flavonoids to be tested under the corresponding planting conditions is taken as the content of total flavonoids under the current planting conditions. The wavelength of the ultraviolet-visible spectrophotometer is set to 500 nm.

[0088] The content of total polysaccharide is detected as follows:

[0089] Respectively, take 0 μL, 25 μL, 50 μL, 100 μL, 200 μL, and 400 μL of glucose reference substance and add water to make up to 0.4 ml. Put the water added glucose reference substance and 0.4 ml of total polysaccharide to be tested into different glass test tubes. Respectively, add 1 ml of 5% phenol solution and 5 ml of 98% concentrated sulfuric acid to each glass test tube. Mix well, then boil in water bath for 30 min. Stand and cool to room temperature. Take 1.5 ml of supernatant and add to a quartz cuvette. Respectively, detect the linear regression equation of the absorbance formed by total polysaccharide using the ultraviolet-visible spectrophotometer. Obtain the concentration of total polysaccharide in the total polysaccharide to be tested according to the linear regression equation of total polysaccharide, multiply by the third dilution coefficient, and the content of total polysaccharide in the total polysaccharide to be tested is obtained. The average of the contents of total polysaccharide in all total polysaccharide to be tested under the corresponding planting conditions is taken as the content of total polysaccharide under the current planting conditions. The wavelength of the ultraviolet-visible spectrophotometer is set to 490 nm.

[0090] The content of kaempferol-3-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside and kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside is detected, and the detection is as follows:

[0091] The control kaempferol-3-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside and kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside are weighed in a volumetric flask, and a mixed control solution is prepared, in which the concentration of kaempferol-3-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside is 25 μg / ml, and the concentration of kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside is 15 μg / ml; the mixed control solution and the to-be-detected solution of kaempferol-3-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside and kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside are eluted with acetonitrile (A flow channel phase) and 0.085% phosphoric acid water (B flow channel phase) = 12:88 isocratic elution, the detection wavelength is 348 nm, the flow rate is 1.0 ml / min, and the column temperature is 30°C; the content of kaempferol-3-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside and the content of kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside in the to-be-detected solution are calculated according to the isocratic elution results, and the average content of kaempferol-3-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside and the average content of kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside in all to-be-detected solutions under the corresponding planting conditions are respectively taken as the content of kaempferol-3-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside and the content of kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside under the current planting conditions. Among them, the content of kaempferol-3-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside (denoted as F1) and the content of kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside (denoted as F2) in the to-be-detected solution are calculated according to the isocratic elution results, and the specific method is as follows: the concentration of F1 in the first mixed control solution and the peak area of F1, and the peak area of F1 in the to-be-detected solution are used to calculate the concentration of F1 in the to-be-detected solution, and the concentration of F2 in the first mixed control solution and the peak area of F2, and the peak area of F2 in the to-be-detected solution are used to calculate the concentration of F2 in the to-be-detected solution, which all satisfy the formula Wherein, C 对照品1 is the concentration of F1 or F2 in the first mixed control solution, A 对照品1 is the peak area of F1 or F2 in the first mixed control solution, C F is the concentration of F1 or F2 in the corresponding test solution, A F is the peak area of F1 or F2 in the corresponding test solution. Then calculate the content of F1 or F2 in the corresponding test solution, both meet the formula Wherein, M F is the content of F1 or F2 in the corresponding test solution, β F is the fourth dilution coefficient, G F is the weight of the test sample of F1 or F2. Wherein, the test sample of F1 or F2 here is the dry powder used for pretreatment when detecting the content of kaempferol-3-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside and kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside. And the first mixed control solution can be used to evaluate the influence of different planting conditions on the chemical composition content of Gouqizi, and further evaluate the quality of Gouqizi, that is, the closer the concentration of F1 or F2 in Gouqizi is to the concentration of F1 or F2 in the first mixed control solution, the better the quality is.

[0092] The content of catechin, epicatechin, tricin, astragalin and kaempferol is detected as follows:

[0093] Take 1 mg of the reference substance catechin, epicatechin, trilobatin, astragalin, kaempferol, and respectively place them in a 10 ml (preferably 5 ml-10 ml) volumetric flask, add 8 ml of 95% pure methanol, ultrasonic for 30 min, dissolve, and dilute to 10 ml; respectively take an appropriate amount to prepare the second mixed standard solution, so that the final concentrations of catechin, epicatechin, trilobatin, astragalin, and kaempferol are 15.9 μg / ml, 11.3 μg / ml, 7.2 μg / ml, 11.2 μg / ml, and 2.0 μg / ml respectively; use a C18 chromatographic column, gradient elute the second mixed reference solution and the catechin, epicatechin, trilobatin, astragalin, and kaempferol to be tested, with acetonitrile (A phase) and 0.085% phosphoric acid water (B phase) as the mobile phase, the elution gradient of acetonitrile being: 0 min-20 min, 10%-15%; 20 min-40 min, 15%-85%; 40 min-55 min, 35%-100%; the detection wavelength is 278 nm, the flow rate is 1.0 ml / min, and the column temperature is 30°C; according to the isocratic elution results, the contents of catechin, epicatechin, trilobatin, astragalin, and kaempferol in the corresponding to-be-tested solution are calculated, and the average of the contents of catechin, epicatechin, trilobatin, astragalin, and kaempferol in all corresponding to-be-tested solutions under the corresponding planting conditions are respectively taken as the contents of catechin, epicatechin, trilobatin, astragalin, and kaempferol under the current planting conditions. Among them, according to the isocratic elution results, the contents of catechin, epicatechin, trilobatin, astragalin, and kaempferol in the corresponding to-be-tested solution are calculated, which is as follows: for each component, including catechin, epicatechin, trilobatin, astragalin, and kaempferol, taking catechin as an example, the concentration of catechin in the second mixed reference solution and the peak area, and the peak area of catechin in the corresponding to-be-tested solution are used to calculate the concentration of catechin in the corresponding to-be-tested solution, which satisfies the formula , wherein C 对照品2 is the concentration of catechin in the second mixed reference solution, A 对照品2 is the peak area of catechin in the second mixed reference solution, C E is the concentration of catechin in the corresponding to-be-tested solution, and A E is the peak area of catechin in the corresponding to-be-tested solution. Then the content of catechin in the corresponding to-be-tested solution is calculated, which satisfies the formula , wherein M E is the content of catechin in the corresponding to-be-tested solution, β E is the fifth dilution coefficient, and G EThe weight of the catechin test sample, wherein the catechin test sample here is the dry powder used for pretreatment when detecting the content of catechin, and the calculation of the content of other components in the test solution is the same.

[0094] The content of total nitrogen and ammonium nitrogen is detected as follows:

[0095] 1 ml of nitrogen standard solution, total nitrogen test solution, and ammonium nitrogen test solution is respectively measured, 100 μl of mixed accelerator is added and shaken, 100 μl of indicator is then added, shaken, and left for 30 min before being added with water to make up to 5 ml. The absorbance is detected by using an ultraviolet-visible spectrophotometer. The concentration of the total nitrogen test solution is calculated according to the absorbance of the total nitrogen test solution, the absorbance of the nitrogen standard solution, and the concentration of the nitrogen standard solution, which is the content of total nitrogen in the total nitrogen test solution. The concentration of the ammonium nitrogen test solution is calculated according to the absorbance of the ammonium nitrogen test solution, the absorbance of the nitrogen standard solution, and the concentration of the nitrogen standard solution, which is the content of ammonium nitrogen in the ammonium nitrogen test solution. The calculation formula is as follows: , wherein C ns represents the concentration of the total nitrogen test solution or the ammonium nitrogen test solution, A ns represents the absorbance of the total nitrogen test solution or the ammonium nitrogen test solution, C sn represents the concentration of the nitrogen standard solution, A sn represents the absorbance of the nitrogen standard solution, and the average of the content of total nitrogen in all total nitrogen test solutions and the average of the content of ammonium nitrogen in all ammonium nitrogen test solutions under the corresponding planting conditions are respectively taken as the content of total nitrogen and the content of ammonium nitrogen under the current planting conditions. The wavelength of the ultraviolet-visible spectrophotometer is set to 430 nm. The mixed accelerator is potassium sulfate: copper sulfate: selenium = 100: 10: 1 dissolved in 10 times the volume of water. The indicator is methyl red: bromo-chloro-cresol = 1: 5 dissolved in 10 times the volume of ethanol.

[0096] The content of total phosphorus is detected as follows:

[0097] 1 ml of the corresponding phosphorus standard solution and total phosphorus test solution is respectively measured, 100 μl of color developing agent is added and shaken, 100 μl of 0.5% antimony potassium tartrate solution is then added, shaken, and left for 10 min before being added with water to make up to 5 ml. The absorbance is detected by using an ultraviolet-visible spectrophotometer. The concentration of the total phosphorus test solution is calculated according to the absorbance of the total phosphorus test solution, the absorbance of the phosphorus standard solution, and the concentration of the phosphorus standard solution, which is the content of total phosphorus in the total phosphorus test solution. The average of the content of total phosphorus in all total phosphorus test solutions under the corresponding planting conditions is taken as the content of total phosphorus under the current planting conditions. The calculation formula is as follows: , wherein C ps represents the concentration of the total phosphorus test solution, A ps represents the absorbance of the total phosphorus test solution, C sp represents the concentration of the phosphorus standard solution, Asp The absorbance of the phosphorus standard solution was measured by UV-Vis spectrophotometer with wavelength of 700 nm and chromogenic agent of 10 g of ammonium molybdate dissolved in 1 L of water and then 126 ml of concentrated sulfuric acid with concentration of 98%.

[0098] The content of available potassium was detected as follows:

[0099] 1 ml of potassium standard solution and available potassium sample solution was respectively taken, 100 μl of sodium sulfate solution with concentration of 0.5 M was added and shaken, 100 μl of sodium tetraphenylborate solution was added, shaken and left for 10 min, and then water was added to make up to 5 ml. The absorbance was detected by UV-Vis spectrophotometer. The concentration of available potassium sample solution was calculated according to the absorbance of available potassium sample solution, the absorbance of potassium standard solution and the concentration of potassium standard solution, and the content of available potassium in available potassium sample solution was the concentration of available potassium sample solution, as shown in the following formula: Wherein, C ks represents the concentration of available potassium sample solution, A ks represents the absorbance of available potassium sample solution, C sk represents the concentration of potassium standard solution, A sk represents the absorbance of potassium standard solution. The average of the content of available potassium in all available potassium sample solutions under corresponding planting conditions was taken as the content of available potassium under current planting conditions, and the wavelength of UV-Vis spectrophotometer was set to 420 nm.

[0100] The content of iron was detected as follows:

[0101] 100 μl of iron standard solution and iron sample solution was respectively taken, 100 μl of hydroxylamine hydrochloride solution, 200 μl of phenanthroline and 500 μl of acetic acid-sodium acetate buffer solution were sequentially added for coloration, shaken and left for 20 min, and then the absorbance was detected by UV-Vis spectrophotometer. The concentration of iron sample solution was calculated according to the absorbance of iron sample solution, the absorbance of iron standard solution and the concentration of iron standard solution, and the content of iron in iron sample solution was the concentration of iron sample solution, as shown in the following formula: Wherein, C Fes represents the concentration of iron sample solution, A Fes represents the absorbance of iron sample solution, C sFe represents the concentration of iron standard solution, A sFe represents the absorbance of iron standard solution. The average of the content of iron in all iron sample solutions under corresponding planting conditions was taken as the content of iron under current planting conditions, and the wavelength of UV-Vis spectrophotometer was set to 510 nm.

[0102] The content of copper was detected as follows:

[0103] Measure 100 μl of the copper standard solution and the copper test solution respectively, add 500 μl of 0.5 mol / l ammonium citrate solution, 500 μl of 0.1 mol / L EDTA disodium salt solution and 200 μl of carbon tetrachloride in sequence, shake well, then add 0.05 g of sodium diethyldithiocarbamate, shake well and let stand for 20 minutes, and then use a UV-visible spectrophotometer to detect the absorbance. According to the absorbance of the copper test solution, the absorbance of the copper standard solution and the concentration of the copper standard solution, the concentration of the copper test solution is calculated as the copper content in the copper test solution, as shown in the calculation formula , where C Cus Indicates the concentration of copper in the test solution, A Cus Indicates the absorbance of the copper test solution, C sCu Indicates the concentration of copper standard solution, A sCu The absorbance of the copper standard solution is expressed as , and the average of the copper content in all the copper test solutions under the corresponding planting conditions is taken as the copper content under the current planting conditions. The wavelength of the UV-visible spectrophotometer is set to 510 nm.

[0104] Each linear regression equation is formed by drawing a standard curve with absorbance as the ordinate and the concentration of the corresponding item as the abscissa. , where x is the absorbance, a and b are constants, and y is the concentration of the corresponding term. The linear regression equation for total flavonoids is: y = 0.9952x + 0.0018 (R 2 = 0.9994); linear regression equation of total polysaccharides: y = 0.376x -0.0059 (R 2 = 0.9990), R 2 It is expressed as the sum of squares of the differences between the sample points and the mean line. While testing the total nitrogen, total phosphorus, available potassium, ammonium nitrogen, iron, and copper, blank tests of the corresponding reagents were also performed using pure water as a blank to calibrate the UV-Vis spectrophotometer.

[0105] The weight of each item was determined by entropy weight method (EW) according to the content of chemical components under the corresponding planting conditions. The weight of each index was analyzed by entropy weight method: first, the average value of the content of the main components of the soil corresponding to the ash treatment group and the average value of the chemical components of San Ye Qing were obtained by detection, and the data were standardized (positive index processing) by SPSS pro software. The weight of each index (including extract, total flavonoids, total polysaccharides, catechin, epicatechin, syringin, astragalin, kaempferol, kaempferol-3-O-β-D- apiose-(1→2)-β-D-glucoside-7-O-α-L-rhamnose glycoside, kaempferol-3-O-β-D-glucoside-7-O-α-L- rhamnose glycoside) was analyzed by entropy weight method. Seven compounds (catechin, epicatechin, syringin, astragalin, kaempferol, kaempferol-3-O-β-D-apiose-(1→2)-β-D-glucoside-7-O-α-L-rhamnose glycoside, kaempferol-3-O-β-D-glucoside-7-O-α-L-rhamnose glycoside), alcohol-soluble extract, total flavonoids and total polysaccharides were included in the investigation. The weight of kaempferol-3-O-β-D-glucoside-7-O-α-L-rhamnose glycoside (F2) was the largest (15.72%), followed by catechin (12.953%) and kaempferol (12.555%), and the weight of total flavonoids was the smallest (5.717%).

[0106] The quality of San Ye Qing was evaluated by TOPSIS according to the weight of each item under the corresponding planting conditions. The relationship between the quality of San Ye Qing and the main components of the soil in different ash treatment groups was evaluated by TOPSIS: the content of alcohol-soluble extract, seven compounds, total flavonoids, total polysaccharides and the yield of San Ye Qing were included in the evaluation of TOPSIS as positive indexes. The optimal matrix vector and the worst matrix vector were found out using the weight determined by entropy weight method. The positive ideal solution distance D+ and the negative ideal solution distance D- of the evaluation objects were calculated, respectively. The comprehensive degree score index S was calculated according to the positive ideal solution distance D+ and the negative ideal solution distance D-. The comprehensive degree score index S of each ash treatment group was sorted, and the higher the S value, the better the quality of San Ye Qing. The results showed that group B ranked first, followed by group D. The combination of TOPSIS and weight evaluation is well known to those skilled in the art, and will not be described here. For reference, see: Jiang Y, Zhang Z, Kadira T, Wu L, Ye W, Wang Y, Xu Y, Fan Y, Ma J, Tao O, Wang J. Enrichment rule, health risk assessment and quality evaluation method of inorganic elements in Huangqi based on AHP-CRITIC weight TOPSIS model combined with chemometrics [J]. Chinese Inorganic Analysis Chemistry, 1-21.

[0107] The correlation between the quality of Radix Tetrastigae and the soil composition was analyzed by the grey correlation degree method. The grey correlation analysis was performed by taking the yield, the content of kaempferol-3-O-β-D-glucoside-7-O-α-L-rhamnoside (F2), catechin and kaempferol as the mother sequence, and the content of each element in the planting soil as the comparison sequence. The correlation degree > 0.9 was considered to have a high correlation. The results showed that the yield and the content of kaempferol of Radix Tetrastigae had a high correlation with the content of ammonium nitrogen, copper and iron; the content of kaempferol-3-O-β-D-glucoside-7-O-α-L-rhamnoside (F2) and catechin had a high correlation with the content of available potassium.

[0108] The weight of the chemical components of Radix Tetrastigae was analyzed by the entropy weight method. The quality of Radix Tetrastigae in different wood ash treatment groups was evaluated by the TOPSIS method by taking the content of essential elements, trace elements and chemical components as the positive index. The results showed that the evaluation ranking of different wood ash treatment groups was B > D > A > G > F > E > C. In addition, the top 3 chemical components with high weight in the entropy weight analysis and the yield of Radix Tetrastigae were taken as the mother sequence to analyze the influence of the abundance of common elements in the planting soil on the important indicators of the quality evaluation of medicinal materials. The results showed that the yield of cultivated Radix Tetrastigae had a high correlation with the content of total nitrogen, copper and iron in the planting soil, with the correlation degree of 0.996, 0.995 and 0.995, respectively. The content of kaempferol in Radix Tetrastigae also had a high correlation with the content of total nitrogen, copper and iron, with the correlation degree of 0.994, 0.993 and 0.992, respectively. The content of catechin and kaempferol-3-O-β-D-glucoside-7-O-α-L-rhamnoside (F2) had a high correlation with the content of available potassium, with the correlation degree of 0.929 and 0.962, respectively. Therefore, the content of total nitrogen, copper and iron in the planting soil had a great influence on the yield and the content of kaempferol of cultivated Radix Tetrastigae, and the content of available potassium in the planting soil had a great influence on the content of catechin and kaempferol-3-O-β-D-glucoside-7-O-α-L-rhamnoside (F2). In summary, the comprehensive quality of Radix Tetrastigae in group B was the best among the 7 wood ash treatment groups. This method provides technical guidance for the cultivation of Radix Tetrastigae and is conducive to improving the quality of Radix Tetrastigae.

[0109] Table 1 Evaluation results by the technique for order preference by similarity to ideal solution (TOPSIS)

[0110]

[0111] Table 2 Evaluation results by the grey correlation degree method

[0112]

[0113] In combination with the above parameters and Figure 2 ,Figure 3 and Figure 4 It can be seen that, Figure 2 In the F1 and F2 mixed control, that is, the first mixed control solution, the A group of ash treatment, that is, the A group (other ash treatment groups are not shown), the results show that the retention time of F1 in the A group is 8.860 min, and the retention time of F2 is 16.765 min, which has the best quality. Figure 3 In the A, B, C, D, E, F, and G correspond to the A group, the B group, the C group, the D group, the E group, the F group, and the G group, respectively, which are the content detection results of each item in the chemical composition of Caulis Tripterygii, the results show that, compared with the control group (A group), P<0.05, the difference is significant, P<0.01, indicates that the difference is significant; ns indicates P>0.05, the difference is not significant, P is the probability value. Figure 4 The contents of each item in the soil of the seven ash treatment groups, the results show that, compared with the control group (A group), the total nitrogen and ammonium nitrogen content of the B group is significantly increased, the total phosphorus content of the C group, the E group, and the F group is significantly increased, the ammonium nitrogen of the C group and the D group is also significantly increased, the ammonium nitrogen of the F group is significantly reduced, and the rest of the components of each group has no significant difference compared with the control group (A group).

[0114] The technical features of the above-described embodiments can be combined arbitrarily, and in order to make the description concise, all possible combinations of the technical features in the above-described embodiments are not described, however, as long as the combination of the technical features does not exist contradictory, it should be considered as the scope of the present application.

[0115] The above-described embodiments only express the more specific and detailed embodiments described in the present application, but should not be interpreted as limiting the scope of the application. It should be noted that for ordinary skilled in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which are within the scope of the present application. Therefore, the scope of the present application should be subject to the appended claims.

Claims

1. A method for detecting the correlation between the quality of Tripterygium wilfordii and soil components, characterized by: The steps include: S1. Pre-treat the three-leaf green and planting soil under different planting conditions to obtain the corresponding test solutions; S2. The test solution of the clover is respectively tested for the content of chemical components, and the test solution of the planting soil is respectively tested for the content of essential elements and trace elements; S3. Determine the weight of the corresponding item using the entropy weight method according to the content of the chemical components under the corresponding planting conditions; S4. Evaluate the quality of Sanyeqing using the superior and inferior solution distance method according to the weights of each item under the corresponding planting conditions; S5. The yield of Tripterygium wilfordii under the best planting conditions and the contents of the top K items with the highest weights in the chemical components are taken as the parent sequence, and the contents of essential elements and trace elements in the corresponding planting soil are used as the comparison sequence. The grey correlation method is used to determine the correlation between the quality of Tripterygium wilfordii and the soil components. When the correlation exceeds the preset threshold, it is considered that the quality of Tripterygium wilfordii is highly correlated with the corresponding elements in the soil components.

2. The method for detecting the correlation between the quality of Tripterygium wilfordii and soil components as claimed in claim 1, wherein: The chemical components include extracts, total flavonoids, total polysaccharides, catechins, epicatechin, luteolin, astragaloside, kaempferol, kaempferol-3-O-β-D-apioside-(1→2)-β-D-glucoside-7-O-α-L-rhamnoside and kaempferol-3-O-β-D-glucoside-7-O-α-L-rhamnoside; the essential elements include total nitrogen, total phosphorus, available potassium and ammonium nitrogen; and the trace elements include iron and copper.

3. The method for detecting the correlation between the quality of Tripterygium wilfordii and soil components as claimed in claim 2, wherein: The content of the extract is detected by a reflux extraction method, the content of total flavonoids, total polysaccharides, total nitrogen, total phosphorus, available potassium, ammonium nitrogen, iron and copper is detected by an ultraviolet-visible spectrophotometer, and the content of catechin, epicatechin, luteolin, astragaloside, kaempferol, kaempferol-3-O-β-D-apioside-(1→2)-β-D-glucoside-7-O-α-L-rhamnoside and kaempferol-3-O-β-D-glucoside-7-O-α-L-rhamnoside is detected by a high performance liquid chromatography method.

4. The method for detecting the correlation between the quality of Tripterygium wilfordii and soil components as claimed in claim 3, wherein: The content of the extract is detected as follows: Measure 10 ml to 30 ml of the test solution of the extract, place it in an evaporating dish that has been dried to constant weight, evaporate to dryness in a water bath at 90°C to 100°C, dry it in an electric blast drying oven at 100°C to 110°C for 3 hours, then cool it in a desiccator for 0.5 h to 1.0 h, weigh the cooled dry product, and obtain the content of the extract in the test solution of the extract, in units of %, to meet the requirements. , M J is the content of the extract in the test solution, C J is the weight of the dry product, β J is the first dilution factor, G J is the weight of the sample to be leached, W J is the water content of the sample of the extract, in %, and the average of the extract contents in the test solutions of all extracts under the corresponding planting conditions is taken as the extract content under the current planting conditions; The content of total flavonoids is detected as follows: Weigh 5 mg-15 mg of rutin reference substance, add 5 ml-8 ml of 70%-100% methanol and dissolve it by ultrasonication, cool to room temperature, add 70%-100% methanol to the 10 ml mark of the volumetric flask, shake well, and obtain rutin reference substance solution; respectively measure 0 ml, 0.1 ml, 0.2 ml, 0.4 ml, 0.7 ml, and 1.0 ml of rutin reference substance solution and add water to 1 ml, and put the rutin reference substance solution and 0.5 ml-2 ml of total flavonoids test solution after adding water into different volumetric flasks, add 200 μL-500 μL of 5% NaNO2 to each volumetric flask, shake well and react for 5 min-10 min, add 200 μL-500 μL of 10% Al(NO3)3, shake well and react for 5 min-10 min, add 2 ml-5 ml of 4% NaOH and shake well for 20 min-40 min, add water to the 10ml scale line of the volumetric flask, shake and place for 10 min-20 min, obtain the reagent formed by the corresponding rutin reference solution, respectively detect the absorbance by ultraviolet-visible spectrophotometer, form a linear regression equation of total flavonoids according to the absorbance and concentration of different rutin reference solutions, and then substitute the absorbance of the total flavonoids test solution into the linear regression equation of total flavonoids to calculate the concentration of total flavonoids in the test solution of total flavonoids, and then multiply by the second dilution factor, i.e. the content of total flavonoids in the test solution of total flavonoids, and the mean of the content of total flavonoids in the test solution of all total flavonoids under the corresponding planting conditions is used as the content of total flavonoids under the current planting conditions, and the wavelength of the ultraviolet-visible spectrophotometer is set to 490nm-520nm; The content of the total polysaccharide is detected as follows: 0 μL, 25 μL, 50 μL, 100 μL, 200 μL, and 400 μL of glucose reference solution were respectively drawn up and made up to 0.4 ml-1 ml with water. The glucose reference solution made up with water and 0.4 ml-1 ml of the total polysaccharide test solution were placed in different glass test tubes. 1 ml-2 ml of 5% phenol solution and 3 ml-7 ml of 90%-98% concentrated sulfuric acid were added to each glass test tube in sequence. After mixing, the mixture was reacted in a boiling water bath for 30 min-60 min, and then allowed to cool to room temperature. 1.5 ml-2 ml of the supernatant was drawn up. ml is added to a quartz cuvette, and the absorbance is detected respectively by an ultraviolet-visible spectrophotometer. A linear regression equation of total polysaccharides is formed according to the absorbance and concentration of different glucose reference substances. The absorbance of the total polysaccharide test solution is substituted into the linear regression equation of total polysaccharides to calculate the concentration of total polysaccharides in the total polysaccharide test solution, and then multiplied by the third dilution factor to obtain the total polysaccharide content in the total polysaccharide test solution. The average of the total polysaccharide contents in all the total polysaccharide test solutions under the corresponding planting conditions is used as the total polysaccharide content under the current planting conditions. The wavelength of the ultraviolet-visible spectrophotometer is set to 490 nm-520 nm; The content of kaempferol-3-O-β-D-glucoside-(1→2)-β-D-glucoside-7-O-α-L-rhamnoside and kaempferol-3-O-β-D-glucoside-7-O-α-L-rhamnoside is detected as follows: The reference substances kaempferol-3-O-β-D-apioside-(1→2)-β-D-glucoside-7-O-α-L-rhamnoside and kaempferol-3-O-β-D-glucoside-7-O-α-L-rhamnoside were weighed into a volumetric flask to prepare kaempferol-3-O-β-D-apioside-(1→2)-β-D-glucoside-7-O-α-L-rhamnoside at a concentration of 10 μg / ml-30 μg / ml and kaempferol-3-O-β-D-glucoside-7-O-α-L-rhamnoside at a concentration of 5 μg / ml-20 μg / ml. μg / ml of the first mixed standard solution; a reversed-phase ODS column was used for isocratic elution of the first mixed standard solution and the test solutions of kaempferol-3-O-β-D-glucoside-(1→2)-β-D-glucoside-7-O-α-L-rhamnoside and kaempferol-3-O-β-D-glucoside-7-O-α-L-rhamnoside. The mobile phase A was acetonitrile, and the mobile phase B was 0.085% phosphoric acid water, and the ratio of acetonitrile to 0.085% phosphoric acid water was 12:

88. The detection wavelength was 348 nm and the flow rate was 1.0 ml / min, column temperature 25℃-35℃; the content of kaempferol-3-O-β-D-apioside-(1→2)-β-D-glucoside-7-O-α-L-rhamnoside and the content of kaempferol-3-O-β-D-glucoside-7-O-α-L-rhamnoside in the corresponding test solution were calculated according to the isocratic elution results, and the content of kaempferol-3-O-β-D-apioside-(1→2)-β-D-glucoside-7-O-α-L-rhamnoside in all the corresponding test solutions under the corresponding planting conditions was calculated. The mean value of the content of kaempferol-3-O-β-D-glucoside-7-O-α-L-rhamnoside and the mean value of the content of kaempferol-3-O-β-D-glucoside-7-O-α-L-rhamnoside correspond to the content of kaempferol-3-O-β-D-apiosyl-(1→2)-β-D-glucoside-7-O-α-L-rhamnoside and the content of kaempferol-3-O-β-D-glucoside-7-O-α-L-rhamnoside under the current planting conditions, respectively; The content of catechin, epicatechin, luteolin, astragaloside and kaempferol is specifically detected as follows: Weigh 1 mg to 2 mg of each of the reference substances catechin, epicatechin, luteolin, astragaloside, and kaempferol, place them in volumetric flasks, add 4 ml to 8 ml of methanol with a purity greater than or equal to 95%, sonicate and dissolve for 15 min to 60 min, and dilute to 5 ml to 10 ml; take 200 μl to 2000 μl of each and mix well to prepare the second mixed reference solution, so that the final concentrations of the standard substances of catechin, epicatechin, luteolin, astragaloside, and kaempferol are 15.9 μg / ml, 11.3 μg / ml, 7.2 μg / ml, 11.2 μg / ml, 2.0 μg / ml; a C18 column was used for gradient elution of the second mixed reference solution and the test solutions of catechin, epicatechin, luteolin, astragaloside, and kaempferol. Mobile phase A was acetonitrile, and mobile phase B was 0.085% phosphoric acid water. The elution gradient of acetonitrile was: 0 min-20 min, 10%-15%; 20 min-40 min, 15%-85%; 40 min-55 min, 35%-100%; the detection wavelength was 230 nm-360 nm, and the flow rate was 1.0 ml / min, column temperature 25°C-35°C; calculating the catechin content, epicatechin content, luteolin content, astragalin content and kaempferol content in the corresponding test solution according to the isocratic elution result, and using the average value of the catechin content, the average value of the epicatechin content, the average value of the luteolin content, the average value of the astragalin content and the average value of the kaempferol content in all corresponding test solutions under the corresponding planting conditions as the catechin content, epicatechin content, luteolin content, astragalin content and kaempferol content under the current planting conditions, respectively; The content of total nitrogen and ammonium nitrogen is detected as follows: Take 0.5 ml to 1 ml of nitrogen standard solution, total nitrogen test solution, and ammonium nitrogen test solution respectively, add 50 μl to 100 μl of mixed accelerator and shake well, then add 50 μl to 100 μl of indicator, shake well, let stand for 15 min to 60 min, and then add water to make up to 2 ml to 5 ml. ml, adopt ultraviolet-visible spectrophotometer to detect absorbance, calculate the concentration of total nitrogen in the test solution according to the absorbance of total nitrogen, the absorbance of nitrogen standard solution and the concentration of nitrogen standard solution, that is, the content of total nitrogen in the test solution of total nitrogen, and calculate the concentration of ammonium nitrogen in the test solution according to the absorbance of ammonium nitrogen, the absorbance of nitrogen standard solution and the concentration of nitrogen standard solution, that is, the content of ammonium nitrogen in the test solution of ammonium nitrogen, and the mean of the content of total nitrogen in the test solution of all total nitrogen under the corresponding planting conditions and the mean of the content of ammonium nitrogen in the test solution of all ammonium nitrogen are respectively used as the content of total nitrogen and the content of ammonium nitrogen under the current planting conditions, and the wavelength of the ultraviolet-visible spectrophotometer is set to 410 nm-430nm, the mixed accelerator is potassium sulfate: copper sulfate: selenium = 100:10:1 dissolved in 10 times the volume of water; the indicator is methyl red: bromocresol green = 1:5 dissolved in 10 times the volume of ethanol; The total phosphorus content is detected as follows: 0.5 ml-1 ml of the phosphorus standard solution and the total phosphorus test solution were respectively measured, 50 μl-100 μl of the color developer was added and shaken, and then 50 μl-100 μl of 0.5% potassium antimony tartrate solution was added, and the mixture was shaken and allowed to stand for 5 min-10 min, and then water was added to 2 ml-5 ml. The absorbance was detected by ultraviolet-visible spectrophotometer, and the concentration of the total phosphorus test solution was calculated according to the absorbance of the total phosphorus test solution, the absorbance of the phosphorus standard solution, and the concentration of the phosphorus standard solution, which was the total phosphorus content in the total phosphorus test solution. The average of the total phosphorus content in all the total phosphorus test solutions under the corresponding planting conditions was used as the total phosphorus content under the current planting conditions. The wavelength of the ultraviolet-visible spectrophotometer was set to 700 nm. The color developer was 10 g of ammonium molybdate dissolved in 1 L of water and then 126 ml of 90%-98% concentrated sulfuric acid was added; The content of the available potassium is detected as follows: 0.5 ml-1 ml of the potassium standard solution and the effective potassium test solution were respectively measured, 50 μl-100 μl of 0.5 M sodium sulfate solution was added and shaken, 50 μl-100 μl of sodium tetraphenylborate solution was added, and the mixture was shaken and allowed to stand for 5 min-10 min, and then water was added to 2 ml-5 ml. The absorbance was detected by ultraviolet-visible spectrophotometer, and the concentration of the effective potassium test solution was calculated according to the absorbance of the effective potassium test solution, the absorbance of the potassium standard solution, and the concentration of the potassium standard solution, which was the effective potassium content in the effective potassium test solution. The average of the effective potassium contents in all the effective potassium test solutions under the corresponding planting conditions was taken as the effective potassium content under the current planting conditions. The wavelength of the ultraviolet-visible spectrophotometer was set to 420 nm; The iron content detection is as follows: 50 μl-100 μl of the iron standard solution and the iron test solution were measured respectively, and 50 μl-100 μl of hydroxylamine hydrochloride solution, 50 μl-200 μl of phenanthroline and 100 μl-500 μl of acetic acid-sodium acetate buffer were added in sequence for color development. After shaking and standing for 10 min-30 min, the absorbance was detected by ultraviolet-visible spectrophotometer. The concentration of the iron test solution was calculated according to the absorbance of the iron test solution, the absorbance of the iron standard solution and the concentration of the iron standard solution, which was the iron content in the iron test solution. The average of the iron content in all the iron test solutions under the corresponding planting conditions was used as the iron content under the current planting conditions. The wavelength of the ultraviolet-visible spectrophotometer was set to 510 nm. The copper content is detected as follows: 50 μl-100 μl of the copper standard solution and the copper test solution were respectively measured, and 500 μl of 0.1 mol / l-0.5 mol / l ammonium citrate solution, 100 μl-500 μl of 0.05 mol / L-0.1 mol / L EDTA disodium salt solution and 50 μl-200 μl of carbon tetrachloride were added in sequence and shaken well. Then, 0.02 g-0.05 g of sodium diethyldithiocarbamate was added and shaken well. After standing for 10 min-30 min, the absorbance was detected by ultraviolet-visible spectrophotometer. The concentration of the copper test solution was calculated according to the absorbance of the copper test solution, the absorbance of the copper standard solution and the concentration of the copper standard solution, which was the copper content in the copper test solution. The average of the copper content in all the copper test solutions under the corresponding planting conditions was used as the copper content under the current planting conditions. The wavelength of the ultraviolet-visible spectrophotometer was set to 510 nm.

5. The method for detecting the correlation between the quality of Tripterygium wilfordii and soil components as claimed in claim 4, wherein: Each linear regression equation is formed by drawing a standard curve with absorbance as the ordinate and the concentration of the corresponding item as the abscissa. , where x is the absorbance, a and b are constants, and y is the concentration of the corresponding item.

6. The method for detecting the correlation between the quality of Tripterygium wilfordii and soil components as claimed in claim 2, wherein: Before the planting soil pre-treatment, the following operations are also performed: In each experimental area, N planting bags of Tripterygium wilfordii were randomly selected for excavation. The following operations were performed on each planting bag: the planting bag was circumcised 10 cm above the ground, and M sampling points were set at the circumcision opening, 5 cm in diameter from the center of the planting bag. The planting soil at each sampling point was removed of crop roots, insects, and stones, and then air-dried and crushed. The soil was then sieved through a 1 mm to 2 mm pore sieve to select the soil sample, which was then sealed, stored, and labeled. Before the pre-treatment of the three-leaf green, the following operations are also performed: Pick the tubers of the three-leaf green root corresponding to the planting bag, wash and dry them, cut them into 2mm-4mm thick slices, dry them at 55℃, weigh them, and then crush them through a 50-mesh sieve. Mix the coarse powder that cannot pass through the 50-mesh sieve with the fine powder that passes through the 50-mesh sieve, and the proportion of fine powder in the mixed coarse powder and fine powder exceeds 90%, which is recorded as dry powder.

7. The method for detecting the correlation between the quality of Tripterygium wilfordii and soil components according to claim 6, wherein: The pre-processing is as follows: 1) When testing for extractables: Weigh 2 g to 4 g of dry powder into a flat-bottomed extraction flask, add 50 ml to 100 ml of 70% ethanol, seal and weigh. Let stand for 1 hour, then remove the seal and connect a reflux condenser. Heat to boiling and hold for 1 hour. Cool to room temperature, remove the flat-bottomed extraction flask, seal and weigh again. Make up the lost weight with water, shake well, and filter through a drying filter to obtain the extract to be tested. 2) For the determination of total flavonoids, catechins, epicatechin, luteolin, astragaloside, kaempferol, kaempferol-3-O-β-D-apioside-(1→2)-β-D-glucoside-7-O-α-L-rhamnoside, and kaempferol-3-O-β-D-glucoside-7-O-α-L-rhamnoside: Weigh 1 g to 2 g of the dry powder into a flat-bottom extraction bottle, add 25-50 times the volume of 60%-90% methanol, and weigh the mixture. Reflux extract at 80°C-95°C for 1-2 h. After cooling to room temperature, add the corresponding concentration of methanol and filter to obtain the initial test solution, which is the total flavonoid test solution. Then, take 20 ml to 40 ml of the filtrate of the initial test solution and concentrate it in an evaporating dish in an 80°C-100°C water bath to 10 ml to 25 ml to obtain the test solutions of catechin, epicatechin, luteolin, astragaloside, kaempferol, kaempferol-3-O-β-D-apioside-(1→2)-β-D-glucoside-7-O-α-L-rhamnoside, and kaempferol-3-O-β-D-glucoside-7-O-α-L-rhamnoside. 3) When testing total polysaccharides: Weigh 1 g-2 g of dry powder and place it in a flat-bottomed extraction bottle, add 50 ml-100 ml of deionized water, seal and weigh, shake and let stand for 0.5 h-1.0 h, remove the stopper and connect the reflux condenser, reflux and extract at 90 ℃-100 ℃ for 1.5 h-3.0 h, cool to room temperature, remove the flat-bottomed extraction bottle, seal and weigh again, make up the lost weight with water, shake and centrifuge at 3000 rpm-4500 rpm for 5 min-15 min, measure 25 ml-50 ml of supernatant, add 1%-5% α-amylase, shake and hydrolyze at 35 ℃-50 ℃ for 10 min-30 min, then take out, centrifuge at 3000 rpm-4500 rpm for 5 min-15 min, measure 2 ml-10 ml of supernatant, add 3 times-5 times anhydrous ethanol, shake and refrigerate at 0 ℃-4 ℃ for more than 10 hours, and then centrifuge at 3000 rpm-4500 rpm for 5 min-15 min. After centrifugation at 4500 rpm for 5 min-15 min, discard the supernatant, add 5 ml-25 ml of deionized water and mix to dissolve, then add 1 / 3 times to 1 / 5 times the volume of a chloroform-n-butanol mixture with a chloroform:n-butanol ratio of 4:1 and shake to remove protein for 1 h-2 h. The supernatant after deproteinization is the test solution for total polysaccharides; 4) When testing total nitrogen and total phosphorus: Weigh 1 g to 2 g of soil sample and place it in a conical flask. Add 0.5 ml to 2 ml of pure water to wet the sample. Then, add 4 ml to 8 ml of 90% to 98% concentrated sulfuric acid and 0.5 ml to 2 ml of hydrogen peroxide in sequence. Cover the mouth of the conical flask with a bent-neck funnel for digestion until white smoke stops. After cooling to room temperature, transfer the sample to a volumetric flask, dilute to 100 ml with pure water, shake well, and let it stand for 10 min to 20 min. Measure 20 ml to 45 ml of the upper layer solution and place it in a volumetric flask. Add 2 ml to 5 ml of 50% sodium hydroxide solution, dilute to 100 ml with pure water, and shake well to obtain the total nitrogen and total phosphorus test solution. 5) When testing for effective potassium: Weigh 4 g to 8 g of soil sample into an extraction bottle, add 20 ml to 40 ml of water and 1 g to 2 g of ammonium acetate powder, shake well for 10 min to 20 min, and filter to obtain the test solution of available potassium; 6) When testing ammonium nitrogen: Weigh 4 g to 8 g of soil sample into an extraction bottle, add 20 ml to 40 ml of water and 1 g to 2 g of potassium chloride powder, shake well for 10 min to 20 min, and filter to obtain the ammonium nitrogen test solution; 7) When testing iron and copper: Weigh 5 g to 10 g of soil sample and place it in a conical flask. Add 10 ml to 20 ml of diethylamine tributyrate ethylenediamine pentaacetic acid extractant, shake for 0.5 h to 2.0 h, and then filter to obtain the test solution for iron and copper. The diethylamine tributyrate ethylenediamine pentaacetic acid extractant contains 0.005 mol / L diethylenetriamine pentaacetic acid, 0.01 mol / L calcium chloride, and 0.1 mol / L triethanolamine.

8. The method for detecting the correlation between the quality of Tripterygium wilfordii and soil components according to claim 1, wherein: The quality of the three-leaf green is evaluated by the superior and inferior solution distance method based on the weights of each item under the corresponding planting conditions, that is, the yield of the three-leaf green under the corresponding planting conditions and the content of each item in the chemical components are used as positive indicators, and the optimal matrix vector and the worst matrix vector are obtained according to the weights of each item in the chemical components. The comprehensive score index S is then calculated according to the positive ideal solution distance D+ and the negative ideal solution distance D- of the corresponding planting conditions, and then ranked. The higher the value of the comprehensive score index S, the better the quality of the three-leaf green under the corresponding planting conditions.

9. The method for detecting the correlation between the quality of Tripterygium wilfordii and soil components according to claim 1, wherein: The different planting conditions include seven wood ash treatment groups, which are recorded as Group A, Group B, Group C, Group D, Group E, Group F, and Group G in sequence. Among them, Group A added 0 kg of wood ash and served as the control group, Group B added 1 kg of ash base fertilizer, Group C added 2 kg of ash base fertilizer, Group D added 1 kg of ash topdressing, Group E added 2 kg of ash topdressing, Group F added 0.5 kg of ash base fertilizer and 0.5 kg of topdressing, and Group G added 1 kg of ash base fertilizer and 1 kg of topdressing.

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