Plant disease-related protein ta cncg10.2 and gene and application thereof

Abstract

The present application belongs to the field of biotechnology, and particularly relates to a plant disease-related protein TaCNGC10.2, a gene thereof and application. The present application provides a plant disease-related protein TaCNGC10.2, wherein the amino acid sequence of the plant disease-related protein TaCNGC10.2 is as shown in SEQ ID NO:1, or a derived amino acid sequence obtained by substitution, deletion and / or addition of one or more amino acids in the amino acid sequence in 1), wherein the derived amino acid sequence has the activity of the amino acid sequence shown in 1). The plant disease-related gene TaCNGC10.2 can be used for breeding improvement of wheat against stripe rust, and provides a gene resource for breeding wheat materials against stripe rust.

Description

Technical Field

[0001] This invention belongs to the field of biotechnology, and in particular relates to the plant disease-associated protein TaCNGC10.2, its gene, and its applications. Background Technology

[0002] Stripe rust fungi are obligate nutritional parasites that must rely on living wheat cells for survival. They require the absorption of intermediate products to grow and develop. Their urediniospores are dispersed to leaves by wind and, under suitable temperature and humidity conditions, germinate within 4-6 hours to form germ tubes that infect wheat and establish a parasitic relationship. After infecting the host plant, stripe rust fungi absorb nutrients from the host plant through their haustoria and reproduce. During this process, the host plant exhibits symptoms such as yellowing and necrosis, weakening its vitality and significantly reducing photosynthesis. This is accompanied by a decrease in key crop yield indicators such as tiller number, ear number, and seed number, as well as reduced plant height and seed weight and quality. Wheat yields plummet, resulting in severe socioeconomic damage.

[0003] Pathogen infection in plants triggers a defensive response, while simultaneously utilizing host components to regulate its own growth, differentiate infection structures, negatively regulate the plant's immune response, and absorb nutrients from the host, thus causing host susceptibility. Host genes in plants that promote pathogen infection and support affinity interactions are considered susceptibility genes. Mutations or deletions of susceptibility genes limit the pathogen's ability to infect and cause disease, thereby enhancing the host's resistance. Before infection, susceptibility genes promote host recognition and pathogen invasion. In the pre-invasion phase, fungal and oomycete spore germination or bacterial adsorption requires specific structures on the host surface. During invasion, different pathogens use different methods to invade the host. Some directly invade by disrupting physicochemical barriers, while others indirectly invade through pores on the host surface, such as stomata. Most fungi and oomycetes can produce spores that enter cells through a series of pathways and form haustoria to complete colonization. During invasion, susceptibility genes negatively regulate the plant's immune response. After invasion, they promote affinity interactions between the host and pathogen, including the absorption of nutrients from the host to meet the pathogen's metabolic and structural needs. Living parasitic fungi require the support of a host to establish a parasitic relationship and absorb nutrients; in fact, they can severely affect the growth and development of host cells.

[0004] Wheat stripe rust is caused by the wheat stripe rust fungus ( Puccinia stiiformis f. sp. tritici This is a major wheat disease caused by pathogens. Using resistant varieties remains the most economical and effective method for control. Plant pathogens utilize susceptible genes in plants to promote infection; interfering with and mutating these susceptible genes will block the affinity interaction between the host and the pathogen, thus enhancing resistance.

[0005] Therefore, identifying wheat disease-susceptibility genes, understanding the response and signal transduction mechanisms of wheat under stripe rust stress, and improving wheat resistance have become important means for the sustainable control of wheat stripe rust. Summary of the Invention

[0006] This invention uses genetic engineering to screen out the plant susceptibility-related protein TaCNGC10.2 and the plant susceptibility-related gene TaCNGC10.2 that encodes the protein. By performing functional analysis on the protein and gene, the disease resistance mechanism in the interaction between wheat and stripe rust is revealed, providing a solution for the breeding of stripe rust-resistant materials.

[0007] To achieve this technical objective, the present invention adopts the following technical solution.

[0008] In a first aspect, the present invention provides a plant susceptibility-associated protein TaCNGC10.2, the amino acid sequence of which is as follows:

[0009] 1) As shown in SEQ ID NO:1; or

[0010] 2) A derived amino acid sequence obtained by substituting, deleting and / or adding one or more amino acids from the amino acid sequence in 1), said derived amino acid sequence having the activity of the amino acid sequence shown in 1).

[0011] In this invention, in order to facilitate the purification of the plant disease-associated protein TaCNGC10.2 in 1), a tag as shown in Table 1 can be attached to the amino terminus or carboxyl terminus of the protein consisting of the amino acid sequence shown in SEQ ID NO:1.

[0012] In this invention, the plant susceptibility-related protein TaCNGC10.2 in 2) can be artificially synthesized, or its encoding gene can be synthesized first and then expressed biologically. The encoding gene of the plant susceptibility-related protein TaCNGC10.2 in 2) can be obtained by deleting one or several amino acid residues from the codon of the plant susceptibility-related gene TaCNGC10.2 sequence as shown in SEQ ID NO:2, and / or by performing a missense mutation of one or several base pairs, and / or by attaching the coding sequence of the tag shown in Table 1 to its 5' end and / or 3' end.

[0013] Table 1. Sequence of Labels

[0014]

[0015] Secondly, a plant susceptibility-related gene TaCNGC10.2 is provided, wherein the plant susceptibility-related gene TaCNGC10.2 is:

[0016] a) Encodes the plant disease-associated protein TaCNGC10.2 described in this invention;

[0017] b) The CDS sequence is shown in SEQ ID NO:2; or

[0018] c) is complementary to a) or b).

[0019] In this invention, the plant disease-related gene TaCNGC10.2 sequence has at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% homology and encodes a DNA molecule with the same function.

[0020] In this invention, those skilled in the art can readily employ known methods, such as directed evolution and point mutation, to mutate the plant disease-related gene TaCNGC10.2, which encodes the plant disease-related protein TaCNGC10.2. Artificially modified polynucleotides possessing 75% or higher sequence identity with the plant disease-related gene TaCNGC10.2 sequence isolated from this invention, as long as they encode the plant disease-related protein TaCNGC10.2 and have the same function, are all derived from and equivalent to the sequence of the plant disease-related gene TaCNGC10.2 of this invention.

[0021] In this invention, the term "identity" refers to sequence similarity to a natural nucleic acid sequence. "Identity" includes nucleotide sequences having 75% or higher, 85% or higher, 90% or higher, or 95% or higher identity with a polynucleotide sequence encoding a protein as shown in SEQ ID NO:1. Identity can be evaluated visually or using computer software. Using computer software, the identity between two or more sequences can be expressed as a percentage (%), which can be used to evaluate the identity between related sequences. 75% or higher identity can be 80%, 85%, 90%, or 95% or higher.

[0022] Thirdly, a polynucleotide construct or host cell containing the plant disease-related gene TaCNGC10.2 as described in this invention is provided.

[0023] Fourthly, a recombinant vector, expression cassette, or recombinant bacteria containing the plant disease-related gene TaCNGC10.2 as described in this invention is provided.

[0024] In this invention, an expression cassette refers to DNA capable of expressing the plant disease-associated gene TaCNGC10.2 as shown in SEQ ID NO:2 in host cells. This DNA may include not only a promoter to initiate polynucleotide transcription as shown in SEQ ID NO:2, but also a terminator to terminate transcription of the plant disease-associated gene TaCNGC10.2 as shown in SEQ ID NO:2. Furthermore, the expression cassette may also include an enhancer sequence. Promoters that can be used in this invention include, but are not limited to: constitutive promoters; tissue-, organ-, and development-specific promoters; and inducible promoters. Suitable transcription terminators include, but are not limited to: Agrobacterium carmine synthase terminator (NOS terminator), cauliflower mosaic virus CaMV 35S terminator, tml terminator, pea rbcS E9 terminator, and carmine and octopine synthase terminator.

[0025] In this invention, recombinant vectors containing the expression cassette can be constructed using existing expression vectors. The plant expression vectors include vectors suitable for plant microbombardment, such as pAHC25, pBin438, pCAMBIA1302, pCAMBIA2300, pCAMBIA2301, pCAMBIA1305, pCAMBIA1300, pBI121, pCAMBIA1391-Xa, or pCAMBIA1391-Xb (CAMBIA). The plant expression vectors may also contain 3-cell structures of exogenous genes. ’ The terminal untranslated region contains the polyadenylated signal and any other DNA fragments involved in mRNA processing or gene expression. The polyadenylated signal guides the addition of polyadenylated DNA to the 3' end of the mRNA precursor. ’ End-of-cell structures, such as Agrobacterium crown gall induction (Ti) plasmid genes (e.g., the carmine synthase gene Nos), and plant genes (e.g., soybean storage protein genes). ’The untranslated regions of terminal transcription all have similar functions. When constructing plant expression vectors using the genes of this invention, enhancers, including translational enhancers or transcriptional enhancers, can also be used. These enhancer regions can be ATG start codons or adjacent region start codons, etc., but must be identical to the reading frame of the coding sequence to ensure correct translation of the entire sequence. The sources of the translation control signals and start codons are wide-ranging; they can be natural or synthetic. The translation initiation region can originate from the transcription initiation region or structural genes. To facilitate the identification and screening of transgenic plant cells or plants, the plant expression vectors used can be processed. This can involve adding genes that can be expressed in plants, encoding enzymes or luminescent compounds that produce color changes (GUS genes, luciferase genes, etc.), antibiotic marker genes (such as the nptII gene for resistance to kanamycin and related antibiotics, the bar gene for resistance to the herbicide phosphinic acid, the hph gene for resistance to the antibiotic hygromycin, the dhfr gene for resistance to methotrexate, and the EPSPS gene for resistance to glyphosate), or chemical reagent resistance marker genes (such as herbicide resistance genes), and mannose-6-phosphate isomerase genes that provide the ability to metabolize mannose. From a safety perspective, transgenic plants can be screened directly under stress without adding any selective marker genes.

[0026] In this invention, the vector can be a plasmid, a granule, a bacteriophage, or a viral vector.

[0027] In this invention, the microorganism can be yeast, bacteria, algae or fungi, such as Agrobacterium.

[0028] In this invention, the transgenic plant cell lines do not include propagation material.

[0029] Fifthly, the invention provides the application of the polynucleotide constructs or host cells described herein in regulating plant disease resistance.

[0030] Sixthly, the invention provides the application of the polynucleotide constructs or host cells described herein in the cultivation of disease-resistant plants.

[0031] In a seventh aspect, the present invention provides the application of the recombinant vector, expression cassette, or recombinant bacteria described herein in regulating plant disease resistance.

[0032] Eighthly, the invention provides the application of the recombinant vector, expression cassette, or recombinant bacteria described herein in the cultivation of disease-resistant plants.

[0033] Preferably, the disease resistance is resistance to stripe rust.

[0034] In this invention, the improvement of plant resistance to stripe rust is specifically reflected in any one of the following (1)-(3): (1) Under stripe rust stress, the sporulation of stripe rust infecting knockout mutant wheat is lower than that in recipient plants; (2) Under stripe rust stress, the expression level of disease-related genes in knockout mutant plants is higher than that in recipient plants; (3) Under stripe rust stress, the mycelial infection area of ​​stripe rust infecting knockout mutant wheat is lower than that in recipient plants. The stripe rust stress is an affinity treatment, and the affinity treatment uses CYR32 and CYR34 strains. This indicates that wheat plants with suppressed expression of the plant susceptibility-related protein TaCNGC10.2 have reduced susceptibility.

[0035] In this invention, since stripe rust is an obligate parasitic fungus, wheat was chosen as the research object, and the wheat variety (Fielder) was chosen as the recipient of the transgene and the research object.

[0036] Ninthly, a method for cultivating transgenic plants with enhanced disease resistance is provided, the method comprising the following steps:

[0037] (1) Reduce or eliminate the content and / or activity of the plant susceptibility-related protein TaCNGC10.2 described in this invention in the target plant to obtain a transgenic plant; or

[0038] (2) Reduce or eliminate the expression of the plant susceptibility-related gene TaCNGC10.2 encoding the plant susceptibility-related protein TaCNGC10.2 described in this invention in the target plant to obtain transgenic plants;

[0039] The transgenic plant exhibits higher disease resistance than the target plant.

[0040] Preferably, the reduction or elimination of the content and / or activity of the plant disease-associated protein TaCNGC10.2 described in this invention in the target plant is achieved by disrupting the expression of the plant disease-associated gene TaCNGC10.2 described in this invention in the target plant.

[0041] Preferably, the reduction or elimination of the expression of the plant disease-related gene TaCNGC10.2 encoding the plant disease-related protein TaCNGC10.2 described in this invention in the target plant is achieved by disrupting the expression of the plant disease-related gene TaCNGC10.2 described in this invention in the target plant.

[0042] In this invention, the target gene of the plant susceptibility-associated protein TaCNGC10.2 (i.e., SEQ ID NO:2 from 5) ’Nucleotides shown in 943-957 were introduced into Agrobacterium EHA105 via the recombinant vector Cas9-TaCNGC10.2 containing an expression cassette of the gene encoding the plant susceptibility-associated protein TaCNGC10.2. The recombinant vector Cas9-TaCNGC10.2 was created by inserting a DNA fragment of the plant susceptibility-associated gene TaCNGC10.2 into the Cas9 vector using homologous recombination while maintaining the other sequences of the Cas9 vector unchanged.

[0043] In this invention, the transgenic plant is understood to include not only the first-generation transgenic plant obtained by transforming the plant susceptibility-related gene TaCNGC10.2 into a recipient plant, but also its progeny. For transgenic plants, the gene can be propagated within the species, or it can be transferred into other varieties of the same species using conventional breeding techniques, particularly commercial varieties. The transgenic plant includes seeds, callus tissue, intact plants, and cells.

[0044] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0045] The plant disease susceptibility-related gene TaCNGC10.2, verified by this invention, is induced to express by stripe rust fungus. Furthermore, transgenic wheat obtained by knocking out TaCNGC10.2 exhibits higher resistance to multiple physiological races of stripe rust fungus compared to wild-type wheat. The proteins and genes provided by this invention offer a foundation for the artificial control of disease resistance-related gene expression and will play a crucial role in cultivating broadly resistant plants.

[0046] This invention clarifies the role of the plant susceptibility-related gene TaCNGC10.2 in the interaction between wheat and stripe rust fungus. Experiments have demonstrated that the plant susceptibility-related gene TaCNGC10.2 can be used for breeding and improving wheat resistance to stripe rust. The plant susceptibility-related gene TaCNGC10.2 provided by this invention offers a genetic resource for cultivating wheat materials resistant to stripe rust. Attached Figure Description

[0047] To more clearly illustrate the technical solution of the present invention, the accompanying drawings involved in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings in the description are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0048] Figure 1 The plant susceptibility-related gene TaCNGC10.2 ( TaCNGC10.2 Expression characteristics of wheat in the wheat-striped rust interaction combination.

[0049] Figure 2 The plant susceptibility-related gene TaCNGC10.2 (TaCNGC10.2 Sequencing identification results of the knockout mutant wheat.

[0050] Figure 3 The plant susceptibility-related gene TaCNGC10.2 ( TaCNGC10.2 Disease resistance identification of knockout mutant wheat. Detailed Implementation

[0051] The technical solution of the present invention will be described below with reference to embodiments. However, the present invention is not limited to the following embodiments. Unless otherwise specified, the experimental methods and detection methods described in each embodiment are conventional methods; unless otherwise specified, the reagents and materials can be purchased commercially.

[0052] The wheat stripe rust physiological race CYR23 in this embodiment of the invention has been disclosed in the literature “Liu P, Guo J, Zhang R, et al. TaCIPK10 interacts with and phosphorylates TaNH2 to activate wheat defense responses to stripe rust[J]. Plant biotechnology journal, 2019, 17(5): 956-968.” It is publicly available from the applicant.

[0053] The wheat stripe rust race CYR31 in this embodiment of the invention has been disclosed in the literature “Wang Fengle, Wu Liren, Xu Shichang, Jin Shelin, Jia Qiuzhen, Yuan Wenhuan, Yang Jiaxiu. Study on new stripe rust races CYR30 and CYR31 in China [J]. Acta Phytoprotectica Sinica, 1996(01):39-44.” It is available to the public from the applicant.

[0054] The wheat variety Shuiyuan 11 in this embodiment of the invention has been disclosed in the literature “Cao Zhangjun, Jing Jinxue, Wang Meinan, et al. Analysis of the relationship between the stripe rust resistance genes of important domestic resistance-resistant varieties Shuiyuan 11, Shuiyuan 92 and Hybrid46 [J]. Northwest Botanical Journal, 2003, 23(1):64-68.” It is available to the public from the applicant.

[0055] Example 1

[0056] This embodiment provides the method for obtaining the plant disease-associated protein TaCNGC10.2 (TaCNGC10.2) and its encoding gene.

[0057] I. Isolation of mRNA and TaCNGC10.2, a gene related to plant disease susceptibility ( TaCNGC10.2 amplification of )

[0058] Take 7-day-old wheat seedlings of the Shuiyuan 11 variety that are growing normally, quick-freeze them with liquid nitrogen, and store them at -80℃ for later use.

[0059] Total RNA was extracted from wheat leaves using a polysaccharide-polyphenol plant RNA extraction kit (Huayueyang Biotechnology Co., Ltd.). First-strand cDNA synthesis was performed using reverse transcriptase XL (AMV). cDNA was synthesized using the SMART method, and the PCR products were detected by 1.0% agarose gel electrophoresis. The amplification primers were:

[0060] TaCNGC10.2 -F:5 ’ -ATGGCGCTCCGCAGGCAGCGGACGT-3 ’ ;

[0061] TaCNGC10.2 -R:5 ’ -TTAGTCTTTTGGCTTGGGCAGCAGA-3 ’ .

[0062] A PCR product of 1959 bp was obtained. Sequencing revealed that this PCR product contained the nucleotides shown in positions 1-1959 of SEQ ID NO:2, and the gene represented by these nucleotides was named the plant disease-associated gene TaCNGC10.2. TaCNGC10.2 ); TaCNGC10.2 The amino acid sequence of the encoded protein is shown in SEQ ID NO:1. The protein is named plant disease-associated protein TaCNGC10.2 (TaCNGC10.2).

[0063] II. RT-qPCR detection TaCNGC10.2 Expression induced by stripe rust fungi

[0064] 1. Preparation of experimental materials

[0065] Wheat variety Shuiyuan 11 leaves were inoculated with CYR23 (incompatible) or CYR31 (compatible) to form incompatible and compatible interaction combinations, respectively, and sterile water was used as a control.

[0066] Samples were taken at 0 h, 6 h, 12 h, 24 h, 48 h, 72 h, 96 h, and 120 h post-inoculation, with the control sampling time points consistent with the treatment. Fresh leaves were cut, wrapped in aluminum foil, flash-frozen in liquid nitrogen, and then stored at -80℃ for later use. Total RNA was extracted from wheat leaves using the Trizol method, and first-strand cDNA was synthesized using reverse transcriptase XL (AMV). cDNA was synthesized using the SMART method.

[0067] 2. RT-qPCR detection TaCNGC10.2 expression level

[0068] According to wheat TaCNGC10.2 Genes and elongation factor genes TaEF We designed specific quantitative PCR primers based on the sequence (GenBank accession number: U76744).

[0069] The RT-PCR primer sequences are:

[0070] TaCNGC10.2 -RT-F:5 ’ -CTGCAGCACACTTTCCGCTACTACT-3 ’ ;

[0071] TaCNGC10.2 -RT-R: 5 ’ -TTCTTCCGTGGAGGCCCTTCTTCAC-3 ’ ;

[0072] TaEF -F:5 ’ -TGGTGTCATCAAGCCTGGTATGGT-3 ’ ;

[0073] TaEF -R:5 ’ -ACTCATGGTGCATCTCAACGGACT-3 ’ .

[0074] Before using quantitative PCR primers, the specificity and amplification efficiency (≥90%) of their amplification products must be tested. TaEF-1α The gene was used as an internal control in real-time PCR analysis. Using an AceQ Universal SYBR qPCR Master Mix (Vazyme, Nanjing, China) and a Bio-Rad CFX Manager quantitative PCR instrument (Bio-rad, Hercules, California), real-time quantitative PCR amplification was performed using cDNA from each treatment sampling site as a template, following the manufacturer's instructions. Each reaction was performed in at least three replicates. The Ct values, mean, and standard deviation of each replicate were generated by the quantitative PCR instrument by manually adjusting the baseline. The Delta-Ct method was used to analyze the experimental data and determine the relative expression level of the gene.

[0075] The results of RT-qPCR are as follows Figure 1 As shown, TaCNGC10.2 The expression patterns of wheat variety Shuiyuan 11 after inoculation with the non-compatible race CYR23 and the compatible race CYR31 of stripe rust, respectively, were analyzed. TaCNGC10.2Upregulated expression was observed in both the incompatible and compatible combinations during the pre-infection phase after inoculation. Figure 1 show, TaCNGC10.2 Expression induced by stripe rust fungi.

[0076] Example 2

[0077] This embodiment provides TaCNGC10.2 Application of knockout mutants in improving plant resistance to stripe rust

[0078] I. TaCNGC10.2 Obtaining knockout mutant wheat

[0079] 1. TaCNGC10.2 Preparation of knockout mutant wheat

[0080] Recombinant vector TaCNGC10.2 -Cas9 used Agrobacterium to infect wild-type wheat Fielder callus to obtain T0 generation transgenic wheat, which was then cultured until sequencing verification was achieved. TaCNGC10.2 Two knockout mutant wheat lines were identified.

[0081] 2 、TaCNGC10.2 Sequencing validation of knockout mutant wheat

[0082] Extract using CTAB method TaCNGC10.2 Genomic DNA was extracted from leaves of two lines of the mutant wheat and the wild-type control wheat (Fielder). TaCNGC10.2 Target primer detection F: 5 ’ -CAGAAATGGGCTAGTACGACTGA-3 ’ R: 5 ’ -CAAGAAACCCTGAGCAGGCGCTT-3 ’ right TaCNGC10.2 Knockout mutant wheat plants were sequenced for identification (upstream primer before the target site, downstream primer after the target site). Fielder was used as a wild-type control.

[0083] The results are as follows Figure 2 As shown, we obtain TaCNGC10.2 Two lines of knockout mutant wheat ( TaCNGC10.2 mutant wheat lines 1 and TaCNGC10.2 Mutant wheat lines 2) are all knockout mutant lines, named TaCNGC10.2 Knockout strain 1 TaCNGC10.2 Knockout strain 2.

[0084] two, TaCNGC10.2 Analysis of stripe rust resistance in mutant wheat

[0085] Wild-type wheat Fielder (material sourced from the College of Plant Protection, Northwest A&F University)TaCNGC10.2 Knockout strain 1 and TaCNGC10.2 Knockout strain 2 was planted on 25 / 23 o After the second leaf unfolds in an incubator with day / night temperature and a 16-hour light / 8-hour dark light cycle (C), Figure 3 In section A), the bacteria were inoculated with physiological races CYR32 and CYR34 of stripe rust (the strains were obtained from the College of Plant Protection, Northwest A&F University). The inoculation method was in accordance with the literature "Kang Zhensheng, Li Zhenqi. Discovery of a new pathogenic strain of Lovlin 10 at room temperature [J]. Journal of Northwest A&F University (Natural Science Edition), 1984(04):18-28." The disease phenotype was observed 14 days after inoculation.

[0086] Phenotypic results are as follows Figure 3 As shown in Figure A, under the infection conditions of stripe rust fungi CYR32 and CYR34, a large number of spore masses were observed on the leaves of all treatments in the control plants (Fielder). TaCNGC10.2 Knockout strain 1 TaCNGC10.2 The number of uredinia on the leaves of the knockout strain 2 wheat was significantly reduced, and the area of ​​chlorotic necrosis was increased.

[0087] III. Biomass Detection

[0088] Stripe rust biomass was measured in wheat 7 days after inoculation. The biomass measurement method followed the guidelines in the literature "Qi Tuo et al., Stripe Rust Effector PstGSRE1 Disrupts Nuclear Localization of ROS-Promoting Transcription Factor TaLOL2 to Defeat ROS-Induced Defense in Wheat, 2019". Results are as follows. Figure 3 As shown in B, it was found that TaCNGC10.2 Knockout strain 1 and TaCNGC10.2 The biomass of stripe rust fungi was significantly reduced in knockout strain 2.

[0089] The above disease resistance identification results indicate that under the treatment conditions of CYR32 and CYR34, TaCNGC10.2 The mutant wheat showed strong resistance, which is a significant advantage in production applications.

[0090] This proves that the plant susceptibility-related gene TaCNGC10.2 ( TaCNGC10.2 ) is an important gene involved in the wheat stripe rust response process.

[0091] The embodiments described above are some, but not all, of the embodiments of the present invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the claimed invention, but rather to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art through related deductions and substitutions based on the inventive concept, without inventive effort, are within the scope of protection of the present invention.

Claims

1. Use of a polynucleotide construct or a host cell in regulating plant resistance to stripe rust, characterized in that, the polynucleotide construct or the host cell contains a plant disease-related gene TaCNGC10.2; the plant disease-related gene TaCNGC10.2 encodes a plant disease-related protein TaCNGC10.2, the CDS sequence of the plant disease-related gene TaCNGC10.2 is shown as SEQ ID NO: 2; the amino acid sequence of the plant disease-related protein TaCNGC10.2 is shown as SEQ ID NO: 1; the regulation is to knockout the expression of the plant disease-related gene TaCNGC10.2 in plants to obtain a transgenic plant; knocking out the plant disease-related gene TaCNGC10.2 can improve the resistance of the transgenic plant to stripe rust; the plant is wheat.

2. Use of a polynucleotide construct or a host cell in breeding a plant resistant to stripe rust, characterized in that, the polynucleotide construct or the host cell contains a plant disease-related gene TaCNGC10.2; the plant disease-related gene TaCNGC10.2 encodes a plant disease-related protein TaCNGC10.2, the CDS sequence of the plant disease-related gene TaCNGC10.2 is shown as SEQ ID NO: 2; the amino acid sequence of the plant disease-related protein TaCNGC10.2 is shown as SEQ ID NO: 1; the breeding is to knockout the expression of the plant disease-related gene TaCNGC10.2 in plants to obtain a transgenic plant; knocking out the plant disease-related gene TaCNGC10.2 can improve the resistance of the transgenic plant to stripe rust; the plant is wheat.

3. Use of a recombinant vector, an expression cassette or a recombinant bacteria in regulating plant resistance to stripe rust, characterized in that, the recombinant vector, the expression cassette or the recombinant bacteria contains a plant disease-related gene TaCNGC10.2; the plant disease-related gene TaCNGC10.2 encodes a plant disease-related protein TaCNGC10.2, the CDS sequence of the plant disease-related gene TaCNGC10.2 is shown as SEQ ID NO: 2; the amino acid sequence of the plant disease-related protein TaCNGC10.2 is shown as SEQ ID NO: 1; the regulation is to knockout the expression of the plant disease-related gene TaCNGC10.2 in plants to obtain a transgenic plant; knocking out the plant disease-related gene TaCNGC10.2 can improve the resistance of the transgenic plant to stripe rust; the plant is wheat.

4. Use of a recombinant vector, an expression cassette or a recombinant bacteria in breeding a plant resistant to stripe rust, characterized in that, the recombinant vector, the expression cassette or the recombinant bacteria contains a plant disease-related gene TaCNGC10.2; The plant disease-related gene TaCNGC10.2 encodes a plant disease-related protein TaCNGC10.2, the CDS sequence of the plant disease-related gene TaCNGC10.2 is shown as SEQ ID NO: 2; the amino acid sequence of the plant disease-related protein TaCNGC10.2 is shown as SEQ ID NO: 1; The cultivation is to knockout the expression of the plant disease-related gene TaCNGC10.2 in plants to obtain a transgenic plant; Knocking out the plant disease-related gene TaCNGC10.2 can improve the resistance of the transgenic plant to stripe rust; The plant is wheat.

5. A method of breeding transgenic plants with increased resistance to stripe rust, comprising, The method comprises the following steps: (1) reducing or eliminating the content and / or activity of the plant disease-related protein TaCNGC10.2 in a target plant to obtain a transgenic plant; or (2) reducing or eliminating the expression of the plant disease-related gene TaCNGC10.2 encoding the plant disease-related protein TaCNGC10.2 in a target plant to obtain a transgenic plant; The transgenic plant has higher resistance to stripe rust than the target plant; The amino acid sequence of the plant disease-related protein TaCNGC10.2 is shown as SEQ ID NO: 1; the plant disease-related gene TaCNGC10.2 encodes a plant disease-related protein TaCNGC10.2, and the CDS sequence of the plant disease-related gene TaCNGC10.2 is shown as SEQ ID NO: 2; The cultivation is to knockout the expression of the plant disease-related gene TaCNGC10.2 in plants to obtain a transgenic plant; Knocking out the plant disease-related gene TaCNGC10.2 can improve the resistance of the transgenic plant to stripe rust; The plant is wheat.

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