Method for preparing L-glufosinate-ammonium by splitting racemic glufosinate-ammonium through electrochemical multienzyme molecular machine

By monitoring current changes through an electrochemical multi-enzyme molecular machine and combining the synergistic effect of specific enzymes, the problem of difficulty in monitoring the process of biological enzymatic separation of D,L-glufosinate has been solved, and efficient and controllable L-glufosinate preparation has been achieved.

CN120829940APending Publication Date: 2025-10-24NANKAI UNIV
1 Cites 0 Cited by

Patent Information

Application Number
CN202410466774.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-04-18
Publication Date
2025-10-24

AI Technical Summary

Technical Problem

Existing enzymatic methods for the separation of D,L-glufosinate lack effective detection methods, making it impossible to achieve controllable and visual monitoring of the reaction process, resulting in low separation efficiency.

Method used

An electrochemical multi-enzyme molecular machine was used to monitor current changes through a three-electrode system. By combining the synergistic effects of catalase, D-amino acid oxidase, iron oxide protein-NADP+ reductase, and glutamate dehydrogenase, the glufosinate separation process was visualized and controlled.

Benefits of technology

The process of glufosinate resolution has been visualized and made controllable, improving resolution efficiency and achieving highly selective and efficient L-glufosinate preparation, which is suitable for large-scale production.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention relates to a method for preparing L-glufosinate-ammonium by splitting racemic glufosinate-ammonium (D, L-glufosinate-ammonium) through an electrochemical multi-enzyme molecular machine, which comprises the following steps: catalyzing D-glufosinate-ammonium by D-amino acid oxidase to obtain 2-carbonyl-4-[hydroxy (methyl) phosphonyl] butyric acid, and catalyzing by amino acid dehydrogenase to obtain L-glufosinate-ammonium. And an electrochemical multi-enzyme molecular machine is utilized to realize dynamic monitoring of the preparation process. By detecting the current change, the reaction kinetics and process are known, and the enzyme catalysis efficiency is improved; nADP < + > green efficient cyclic regeneration is achieved, separation of an intermediate product is not needed, L-glufosinate-ammonium is directly obtained, the conversion efficiency reaches up to 90% or above, and the enantiomer excess rate is gt; 99%; the method is simple to operate, green, efficient, high in selectivity and suitable for large-scale popularization and demonstration of L-glufosinate-ammonium.
Need to check novelty before this filing date? Find Prior Art