High-voltage cooperative pulse generation system

Abstract

The invention discloses a high-voltage collaborative pulse generation system which comprises a user interface, a control unit, a driving circuit, a charging module and a collaborative pulse generation device. The cooperative pulse generation device is a Marx generator; the charging module charges a high-voltage pulse main capacitor bank of the Marx generator according to the obtained high-voltage control signal so as to obtain a high-voltage processing pulse; the Buck circuit reduces the voltage of the direct current charging power supply output of the charging module according to the obtained duty ratio and outputs the voltage to a low-voltage pulse main capacitor bank of the Marx generator, the capacitor bank is charged, and a low-voltage processing pulse is obtained; the Marx generator generates high and low voltage pulse signals with different parameters according to the driving signal and the charging control signal; the electrode transmits high-low voltage cooperative pulses generated by the Marx generator circuit to a load to be processed. Only one direct-current charging power supply is needed, part of circuit elements are shared, the utilization efficiency of all the elements is greatly improved, and the manufacturing cost is reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of high-voltage cooperative pulse generation, and particularly relates to a high-voltage cooperative pulse generation system. BACKGROUND

[0002] The biological effects induced by pulsed electromagnetic fields have been increasingly applied in the biomedical field and attracted more and more attention. The electroporation effect induced by high-voltage pulsed electric fields has entered the commercial application stage in many fields. For example, reversible electroporation has been relatively maturely applied in transdermal drug delivery, gene electroporation, cell fusion, and auxiliary electrochemical tumor therapy; irreversible electroporation technology has shown great prospects in the application of tissue ablation, tumor ablation, and cardiac defibrillation. In the application of reversible electroporation, studies have shown that the combination of high-voltage narrow pulses and low-voltage wide pulses can significantly improve the efficiency of gene electroporation and cell fusion. High-voltage pulsed irreversible electroporation tissue ablation relies on the transmission of pulses to the inside of biological tissues by treatment electrodes. Considering biological safety, the distance between treatment electrodes is relatively small (0.5-3 cm) to achieve a relatively high treatment electric field at a relatively low voltage, but this may cause a small treatment area. In particular, in the process of tumor ablation, the size of the tumor that can be ablated by traditional pulses is mostly limited to less than 3 cm. In order to improve the size of pulsed electric field ablation while avoiding the threat to biological safety caused by the increase of pulse voltage, researchers found that the combined use of high-voltage narrow pulses and low-voltage narrow pulses can significantly improve the size of tissue ablation and has been verified by experiments. However, the mechanism of the effect of cooperative pulses on increasing the size of ablation is still inconclusive. The main academic views include the electrolysis effect of low-voltage wide pulses, which increases the size of ablation. Some views also suggest that low-voltage wide pulses can expand the reversible electroporation region outside the ablation region during the action of high-voltage narrow pulses to irreversible electroporation region, thereby expanding the ablation range. Therefore, it is necessary to further study the influence mechanism of cooperative pulse parameters on tissue ablation effect. Therefore, it is particularly necessary to develop a cooperative pulse generation device that can stably generate high-voltage and low-voltage pulses with different pulse widths.

[0003] The existing collaborative pulse generating device adopts a double power supply mode, in which the high-voltage narrow pulse and the low-voltage wide pulse adopt independent charging direct-current power supplies, and the discharge circuits are relatively independent. The collaborative pulse irreversible electroporation device disclosed in the prior art with the publication number CN109124760B generates two kinds of pulses by using two modules with high voltage and low voltage respectively to form collaborative pulses, wherein the high-voltage pulse module and the low-voltage pulse module have their own charging power supplies and discharge circuits. The publication number CN114448396A discloses a collaborative pulse generating device and method, in which the low-voltage pulse module is charged by a low-voltage direct-current power supply, the high-voltage pulse module is boosted by a power supply through a step-up transformer, and then a high-voltage direct-current input is obtained by rectification to charge the high-voltage module capacitor. Essentially, it is still a mode of independent charging and independent discharging of high-voltage and low-voltage modules. The publication number CN110071707B discloses a pulse signal generating device, which also separately uses a high-voltage direct-current module to charge the high-voltage module capacitor and a low-voltage direct-current power supply to charge the low-voltage module capacitor, and the discharge circuits are respectively the processes of discharging the load by the series connection of the high-voltage module capacitor and the series connection of the low-voltage module capacitor. The double power supply collaborative pulse generation mode of the above-mentioned prior art has the advantage of reducing the mutual influence of high-voltage and low-voltage discharges, but the circuit structure is not compact enough, and the use of double power supplies increases the spatial complexity and the manufacturing cost. SUMMARY

[0004] 1. Technical problems to be solved:

[0005] In view of the above technical problems, the present application provides a high-voltage collaborative pulse generating system, which adopts a Marx generator circuit based on only one direct-current charging power supply, shares part of the circuit elements, greatly improves the utilization efficiency of each component, and reduces the manufacturing cost. The amplitude of the high-voltage pulse and the low-voltage pulse can be calculated and determined according to the charging voltage, the number of Marx circuit stages, and the resistance-capacitance voltage division ratio. The main purpose of the present application is to design a collaborative pulse generating device with compact structure, high component utilization rate and relatively low manufacturing cost.

[0006] 2. Technical solutions:

[0007] A high-voltage collaborative pulse generating system, comprising a user interface, a control unit, a driving circuit, a charging module, a collaborative pulse generating device, an electrode and a load to be processed connected in sequence; the charging module comprises a direct-current charging power supply and a Buck circuit; the collaborative pulse generating device is a Marx generator circuit;

[0008] The user sends a cooperative pulse parameter requirement to the control unit through a user interface; the control unit analyzes the cooperative pulse parameter into corresponding high / low voltage pulse control signals and sends them to a charging module and a driving circuit respectively; the driving circuit enhances the obtained driving signal to drive the semiconductor switching device in the Marx generator circuit; the charging module charges the high voltage pulse main capacitor group of the Marx generator according to the obtained high voltage control signal to obtain a high voltage processing pulse; the Buck circuit reduces the output of the DC charging power supply of the charging module to the low voltage pulse main capacitor group of the Marx generator according to the obtained duty cycle to charge the capacitor group and obtain a low voltage processing pulse.

[0009] The Marx generator generates high / low voltage pulse signals with different parameters according to the driving signal and the charging control signal; the electrodes transmit the high / low voltage cooperative pulses generated by the Marx generator circuit to the load to be processed.

[0010] Further, the Marx generator circuit is an n-stage Marx circuit, where n is an integer greater than or equal to 2; the anode of the high voltage pulse circuit isolation diode in the first stage of the Marx circuit is connected to the anode of the DC charging power supply through a current-limiting resistor, the anode of the low voltage pulse circuit isolation diode is connected to the output end of the Buck circuit, and the drain of the high voltage charging switch is connected to the cathode of the DC charging power supply; the anode of the high voltage pulse circuit isolation diode in the next stage circuit after the first stage is connected to the cathode of the isolation diode in the previous stage, the anode of the low voltage pulse circuit isolation diode is connected to the cathode of the low voltage pulse circuit isolation diode in the previous stage, and the drain of the high voltage charging switch is connected to the source of the high voltage charging switch in the previous stage.

[0011] Each stage of the Marx circuit is composed of a high voltage pulse Marx circuit and a low voltage pulse Marx circuit; the high voltage pulse Marx circuit is: the drain of the high voltage discharge switch Sn1 is connected to the cathode of the high voltage pulse circuit isolation diode in the stage, and the source is connected to the source of the charging switch Sn3; one end of the high voltage pulse main capacitor Cn2 is connected to the cathode of the high voltage pulse circuit isolation diode in the stage, and the other end is connected to the drain of the high voltage charging switch Sn3; the low voltage pulse Marx circuit is: the drain of the low voltage discharge switch Sn2 is connected to the cathode of the low voltage pulse circuit isolation diode in the stage, and the source is connected to the source of the charging switch Sn3; one end of the low voltage pulse main capacitor Cn1 is connected to the cathode of the low voltage pulse circuit isolation diode in the stage, and the other end is connected to the drain of the high voltage charging switch Sn3.

[0012] One end of the load is connected to the negative electrode of the DC charging power supply, and the other end is connected to the output of the highest stage of the Marx circuit, i.e. the source of the high / low voltage pulse discharge switch tube in the highest stage.

[0013] Further, the high-voltage charging switch, the high-voltage discharging switch and the low-voltage discharging switch are all full-control solid-state switch tubes.

[0014] Further, when the system charges the high-voltage pulse main capacitor and the low-voltage pulse main capacitor, the high-voltage discharging switch and the low-voltage discharging switch of each stage of the Marx circuit are turned off, and the high-voltage charging switch is turned on; the power supply U1 charges the high-voltage pulse main capacitor of each stage through the current-limiting resistor RC, and the Buck circuit reduces the voltage U1 to U2 to charge the low-voltage pulse main capacitor; after the charging of each stage of the Marx circuit is completed, the voltage of the high-voltage pulse main capacitor is U1, the voltage of the low-voltage pulse main capacitor is U2, and U2 = aU1, wherein a is the duty cycle of the pulse.

[0015] Further, when the system discharges the high-voltage pulse, the high-voltage discharging switch of each stage of the Marx circuit is turned on, and the other switches are turned off; the high-voltage pulse main capacitors in each stage of the Marx circuit are connected in series to discharge the load, and a high-voltage pulse with a voltage of nU1 is obtained, wherein n is the number of stages of the Marx circuit.

[0016] Further, when the system discharges the low-voltage pulse, the low-voltage discharging switch of each stage of the Marx circuit is turned on, and the other switches are turned off; the low-voltage pulse main capacitors in each stage of the Marx circuit are connected in series to discharge the load, and a low-voltage pulse with a voltage of nU1 is obtained.

[0017] 3. Beneficial effects:

[0018] (1) The high-voltage cooperative pulse generation system provided by the method adopts a Buck circuit to reduce the output of a direct-current charging power supply and charge a low-voltage pulse main capacitor, so that only one high-voltage direct-current charging power supply is needed to obtain high-voltage pulses and low-voltage pulses; and because the Buck circuit is used for direct-current voltage reduction, the amplitude of the low-voltage pulse can be adjusted according to the output value of the high-voltage pulse to obtain the duty cycle of the switch of the Buck circuit.

[0019] (2) The high-voltage cooperative pulse generation system provided by the method is based on a traditional Marx circuit structure, the high-voltage pulse main capacitor is charged by a direct-current charging power supply, the low-voltage pulse main capacitor is charged by the output of a Buck circuit, and finally high-voltage pulses and low-voltage pulses with different amplitudes are generated; the cooperative pulse generation device can flexibly adjust each pulse parameter through control of the switch signal, the control signal of the system is simple, the flexible configuration of the high-voltage parameter and the low-voltage parameter can be realized, and the arbitrary cooperation between the high-voltage pulse and the low-voltage pulse can be realized; the high-voltage pulse discharging switch and the low-voltage pulse discharging switch are independently controlled, the flexible adjustment of the respective parameters and the arbitrary cooperation between the high-voltage pulse and the low-voltage pulse can be realized; and only one direct-current power supply is needed for charging, thereby reducing the manufacturing cost. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 It is a system block diagram of the high-voltage cooperative pulse generation system.

[0021] Figure 2 A main topology of a Marx circuit involved in a high-voltage cooperative pulse generation system;

[0022] Figure 3 A Buck circuit involved in a high-voltage cooperative pulse generation system;

[0023] Figure 4 A charging circuit of a cooperative pulse generation device in the method;

[0024] Figure 5 A high-voltage pulse discharge circuit of a cooperative pulse generation device in the method;

[0025] Figure 6 A low-voltage pulse discharge circuit of a cooperative pulse generation device in the method;

[0026] Figure 7 A timing of each switch driving signal and corresponding output in a verification example;

[0027] Figure 8 Different forms of high-voltage and low-voltage pulse outputs in a verification example;

[0028] Figure 9 Different rising edges and falling edges of high-voltage pulses in a verification example;

[0029] Figure 10 Different rising edges and falling edges of high-voltage pulses and low-voltage pulses and their parameter arbitrary combinations in a verification example. DETAILED DESCRIPTION

[0030] The application will be described in detail below with reference to the drawings.

[0031] As shown in the accompanying drawings, Figure 1 A high-voltage cooperative pulse generation system includes a user interface, a control unit, a driving circuit, a charging module, a cooperative pulse generation device, an electrode and a load to be processed connected in sequence; the charging module includes a direct current charging power supply and a Buck circuit; the cooperative pulse generation device is a Marx generator circuit;

[0032] The user sends a cooperative pulse parameter requirement to the control unit through a user interface; the control unit analyzes the cooperative pulse parameter into corresponding high / low voltage pulse control signals and sends them to a charging module and a driving circuit respectively; the driving circuit enhances the obtained driving signal to drive the semiconductor switching device in the Marx generator circuit; the charging module charges the high voltage pulse main capacitor group of the Marx generator according to the obtained high voltage control signal to obtain a high voltage processing pulse; the Buck circuit reduces the output of the DC charging power supply of the charging module to the low voltage pulse main capacitor group of the Marx generator according to the obtained duty cycle to charge the capacitor group and obtain a low voltage processing pulse.

[0033] The Marx generator generates high / low voltage pulse signals with different parameters according to the driving signal and the charging control signal; the electrodes transmit the high / low voltage cooperative pulses generated by the Marx generator circuit to the load to be processed.

[0034] Further, the Marx generator circuit is an n-stage Marx circuit, where n is an integer greater than or equal to 2; the anode of the high voltage pulse circuit isolation diode in the first stage of the Marx circuit is connected to the anode of the DC charging power supply through a current-limiting resistor, the anode of the low voltage pulse circuit isolation diode is connected to the output end of the Buck circuit, and the drain of the high voltage charging switch is connected to the cathode of the DC charging power supply; the anode of the high voltage pulse circuit isolation diode in the next stage circuit after the first stage is connected to the cathode of the isolation diode in the previous stage, the anode of the low voltage pulse circuit isolation diode is connected to the cathode of the low voltage pulse circuit isolation diode in the previous stage, and the drain of the high voltage charging switch is connected to the source of the high voltage charging switch in the previous stage.

[0035] Each stage of the Marx circuit is composed of a high voltage pulse Marx circuit and a low voltage pulse Marx circuit; the high voltage pulse Marx circuit is: the drain of the high voltage discharge switch Sn1 is connected to the cathode of the high voltage pulse circuit isolation diode in the stage, and the source is connected to the source of the charging switch Sn3; one end of the high voltage pulse main capacitor Cn2 is connected to the cathode of the high voltage pulse circuit isolation diode in the stage, and the other end is connected to the drain of the high voltage charging switch Sn3; the low voltage pulse Marx circuit is: the drain of the low voltage discharge switch Sn2 is connected to the cathode of the low voltage pulse circuit isolation diode in the stage, and the source is connected to the source of the charging switch Sn3; one end of the low voltage pulse main capacitor Cn1 is connected to the cathode of the low voltage pulse circuit isolation diode in the stage, and the other end is connected to the drain of the high voltage charging switch Sn3.

[0036] One end of the load is connected to the negative electrode of the DC charging power supply, and the other end is connected to the output of the highest stage of the Marx circuit, i.e. the source of the high / low voltage pulse discharge switch tube in the highest stage.

[0037] Further, the high-voltage charging switch, the high-voltage discharging switch and the low-voltage discharging switch are all full-controlled solid-state switch tubes.

[0038] Further, when the system charges the high-voltage pulse main capacitor and the low-voltage pulse main capacitor, the high-voltage discharging switch and the low-voltage discharging switch of each stage of the Marx circuit are turned off, and the high-voltage charging switch is turned on; the power supply U1 charges the high-voltage pulse main capacitor of each stage through the current-limiting resistor RC, and the Buck circuit reduces the voltage U1 to U2 to charge the low-voltage pulse main capacitor; after the charging of each stage of the Marx circuit is completed, the voltage of the high-voltage pulse main capacitor is U1, the voltage of the low-voltage pulse main capacitor is U2, and U2=αU1, where α≤1, and α is the duty cycle of the pulse.

[0039] Further, when the system discharges the high-voltage pulse, the high-voltage discharging switch of each stage of the Marx circuit is turned on, and the other switches are turned off; the high-voltage pulse main capacitors in each stage of the Marx circuit are connected in series to discharge the load, and a high-voltage pulse with a voltage of nU1 is obtained, where n is the number of stages of the Marx circuit.

[0040] Further, when the system discharges the low-voltage pulse, the low-voltage discharging switch of each stage of the Marx circuit is turned on, and the other switches are turned off; the low-voltage pulse main capacitors in each stage of the Marx circuit are connected in series to discharge the load, and a low-voltage pulse with a voltage of nU1 is obtained.

[0041] The principles and parameters involved in the method will be briefly described below with reference to the accompanying drawings.

[0042] As shown in FIG. 1, the system comprises a power supply U1, a current-limiting resistor RC, a Buck circuit, a high-voltage charging switch, a high-voltage discharging switch, a low-voltage discharging switch and a Marx circuit. Figure 1As shown, the overall structure diagram of the application, the system mainly includes user interface, control unit, direct current charging power supply, drive circuit, Buck circuit, Marx generator circuit, electrode, object to be processed eight parts. Each unit: (1) user interface: user interface is the key interface of human-computer interaction, its main function is to pass the user instruction to the control unit, mainly including high and low voltage pulse parameters. (2) control unit: the control unit mainly converts the user instruction into corresponding pulse control signal, such as high voltage pulse amplitude, pulse width, rising edge, falling edge, frequency, number and corresponding low voltage pulse parameters. (3) direct current charging power supply: mainly used for charging high voltage main capacitor, used for obtaining high voltage processing pulse. (4) drive circuit: the received high and low voltage pulse control signal is converted into the drive signal required by high voltage solid state switch. (5) Buck circuit: Buck circuit mainly reduces the output of direct current charging power supply to low voltage main capacitor, charges the capacitor group, and obtains low voltage processing pulse. (6) Marx generator circuit: mainly used for generating high and low voltage pulse signal, for Marx circuit topology structure, main capacitor is divided into high voltage pulse main capacitor and low voltage pulse main capacitor, high voltage main capacitor is charged by direct current charging power supply, low voltage pulse main capacitor is charged by Buck circuit output, and finally high and low voltage pulses with different amplitudes are generated. (7) electrode: the electrode can transmit the high and low voltage cooperative pulse generated by the Marx generator to the object to be processed; according to the difference of the processing object, different electrodes can be selected, such as plate electrode or needle electrode. (8) object to be processed: according to the difference of application field, the cooperative pulse generating device can be applied to the fields of gene electroporation, cell fusion and tissue ablation. Therefore, the processing object can be cell or biological tissue.

[0043] As shown in the accompanying drawings Figure 2 , 3 , respectively, the Marx generator circuit and the Buck circuit of the cooperative pulse generating device for realizing the method, and the working principle is: the circuit adopts Marx circuit topology structure; the direct current charging power supply voltage of the charging module is U1, and the high voltage pulse amplitude that n level Marx can output is nU1. The direct current charging power supply U1 is also the input voltage of the Buck circuit, the output voltage of the Buck circuit is U2, and the relationship between the Buck circuit output voltage U2 and the input voltage U1 satisfies: U2 = αU1, wherein α is the duty ratio, α ≤ 1; the low voltage pulse output amplitude is nU2.

[0044] In the figure, diode D n1 , D n2 is used for isolation of different potentials in the process of forming high voltage pulse and low voltage pulse, and the reverse withstand voltage value should be higher than the charging voltage. Each charging main capacitor is divided into high voltage pulse main capacitor and low voltage pulse main capacitor, wherein C n1 , C n2They are respectively the low-voltage pulse main capacitor and the high-voltage pulse main capacitor; the equivalent energy storage capacitor after each stage of series connection needs to ensure that when the n-stage series discharge is carried out, the voltage drop of the square wave pulse under the conditions of maximum pulse width output and minimum load does not exceed the set threshold (generally 5% of the output voltage); the withstand voltage value of each stage of capacitance should be higher than the maximum voltage value it withstands during the charging and discharging process.

[0045] In the figure, the high voltage discharge switch S n1 Used to control high voltage pulse parameters. When high voltage pulse is generated, S n1 Closed, S n2 Disconnect, S n3 Disconnect, S n1 The driving signal determines the high voltage pulse width, repetition rate, rising edge and other parameters. n2 Used to control low-voltage pulse parameters. When a low-voltage pulse is generated, S n1 Disconnect, S n2 Closed, S n3 Disconnect, S n2 The driving signal determines the low voltage pulse width, repetition rate, rising edge and other parameters. n3 It is the charging switch. When charging, S n1 Disconnect, S n2 Disconnect, S n3 After the discharge is completed, S n3 Closing can speed up the charge release channel on the load and increase the steepness of the falling edge; R L is the equivalent resistance of the object to be processed. Specific embodiment 1:

[0047] This embodiment is used to illustrate the charging mode of this system; Figure 4 As shown, during the charging phase, the switch S 11 , S 21 ,……,S n1 Disconnect, switch S 12 , S 22 ,……,S n2 Disconnect, switch S 13 , S 23 ,……,S n3 Closed, DC power supply U1 to C 12 , C 22 ,……,C n2 All high voltage pulse main capacitors are finally charged to voltage U1. Buck circuit output U2 to C 11 , C 21 ,……,C n1 Charging, all low-voltage pulse main capacitors are eventually charged to voltage U2, U2 = αU1, α≤1.

[0048] In this embodiment, for different high-voltage pulse output amplitude requirements, it can be realized by controlling the DC charging power output, and the low-voltage pulse amplitude needs to consider the DC charging power output and control the on-duty ratio of switch S0 in the Buck circuit. Embodiment 2:

[0050] This embodiment is used to illustrate the high-voltage pulse discharge phase of the system, and the circuit schematic diagram is as shown in Figure 5 When high-voltage pulse discharge, switches S 11 , S 21 , …, S n1 are closed, switches S 12 , S 22 , …, S n2 are opened, switches S 13 , S 23 , …, S n3 are opened, and the capacitors are discharged in series to the load, and the voltage obtained on the load is a square wave pulse signal of nU1. In the high-voltage pulse discharge phase, capacitors C 12 , C 22 , …, C n2 participate in discharge, and the equivalent discharge capacitance is:

[0051]

[0052] In order to ensure that the voltage drop during high-level discharge does not exceed the set threshold β, then

[0053]

[0054] Where R L is the load resistance, and τ is the high-voltage pulse width. When the maximum high-voltage pulse width and the minimum load resistance are selected, the lower limit value of capacitor C n2 can be calculated.

[0055] The high-voltage pulse width, rising edge, falling edge, and repetition frequency are determined by the driving signals of switches S 11 , S 21 , …, S n1 . Embodiment 3:

[0057] This embodiment is used to illustrate the low-voltage pulse discharge phase of the system, and the circuit schematic diagram of the low-voltage pulse discharge phase is as shown in Figure 6 When low-voltage pulse discharge, switches S 11 , S 21 , …, S n1 are opened, switches S 12 , S 22 , …, S n2 are closed, and switches S 13, S 23 , …, S n3 is disconnected, the capacitor discharges in series to the load, and the voltage on the load is a square wave pulse signal of nαU1. In the low-voltage pulse discharge stage, the capacitor C 11 , C 21 , …, C n1 participates in the discharge, and the equivalent discharge capacitance is:

[0058]

[0059] To ensure that the voltage drop during discharge does not exceed the set threshold β', then

[0060]

[0061] where R L is the load resistance, and τ' is the low-voltage pulse width. When the maximum low-voltage pulse width and the minimum load resistance are selected, the lower limit value of the capacitor C n1 can be calculated.

[0062] The low-voltage pulse width, rising edge, falling edge, and repetition frequency are determined by the driving signals of the switches S 12 , S 22 , …, S n2 . In addition, the coordination mode between high and low voltage pulses, including the high and low voltage pulse interval time and the number of pulses, can also be obtained by controlling the timing of each control switch.

[0063] Verification example:

[0064] To verify that the cooperative pulse generation device of the present scheme can generate specified pulse waveforms, first control the timing of the driving signals of the switches, Figure 7 as shown in Fig. 6, which shows the typical control timing of the three switches S i1 , S i2 , S i3 (i = 1, 2, …, n). Among them, the ab section is the charging stage, the driving signal of the switch S i3 (i = 1, 2, …, n) is high level on, and the driving signal of the switch S i1 , S i2 (i = 1, 2, …, n) is low level off. The bc section is the high-voltage pulse discharge stage, the switches S i1 (i = 1, 2, …, n) are synchronously turned on, and the switches S i2 , S i3 (i = 1, 2, …, n) are turned off. In order to protect the switch device, a certain dead time is left between the switch S i3 and the switch S i1 . The cd section again enters the charging mode, after a certain dead time, enters the de section low-voltage pulse discharge stage, the switches Si2 (i=1, 2, …, n) are turned on synchronously, and the switch S i1 , S i3 (i=1, 2, …, n) are turned off. Figure 7 It is shown that the output high-low voltage pulse waveform is controlled by the control switch driving signal, and the main pulse parameters of the high-low voltage pulse, such as pulse width, pulse interval time and pulse number, can be obtained by controlling the parameters of the control switch driving signal.

[0065] The cooperation mode of any high-low voltage pulse can be realized by controlling the switch driving signal, as shown in Figure 8 . The application order of the high-low voltage pulse, the number of the application of the high-low voltage pulse, and the interval between the high-low voltage pulses can be flexibly adjusted, as shown in Figure 8 (a), (b). In addition, the high-low voltage pulse can also be applied in the form of pulse train, Figure 8 (c) gives the high voltage pulse train, and by controlling the switch driving signal, the low voltage pulse can also be applied in the form of pulse train. The pulse with variable pulse width can be obtained according to the actual demand; (c) gives the application mode of the low voltage pulse with variable pulse width, and in fact, the high voltage pulse can also be realized to output with variable pulse width according to the demand.

[0066] The cooperative pulse generating device proposed in the application can control the rising and falling edges of the output waveform by changing the switch on-off time sequence, as shown in Figure 9 . The rising and falling edges of the output pulse are realized by changing the on-off time sequence of the high voltage discharge switch S i1 (i=1, 2, …, n). Similarly, the rising and falling edges of the low voltage discharge pulse can be controlled.

[0067] In summary, the cooperative pulse generating device proposed in the application can generate high-low voltage pulses with different rising and falling edges and combine them in any mode, as shown in Figure 10 . The square wave pulse with different rising edges contains different frequency contents, and in the application of reversible electroporation and irreversible electroporation, the high-low voltage pulse with different rising edges can be selected according to the frequency spectrum demand to process the biological object.

[0068] Although the application has been disclosed as above with preferred embodiments, they are not intended to limit the application, and any person skilled in the art can make various changes or modifications without departing from the spirit and scope of the application, therefore the protection scope of the application should be defined by the protection scope of the claims of the application.

Claims

1. A high voltage coordinated pulse generation system, characterized by: The system comprises a user interface, a control unit, a driving circuit, a charging module, a collaborative pulse generator, an electrode and a load to be treated, which are connected in sequence. The user sends a collaborative pulse parameter demand to the control unit through the user interface, and the control unit analyzes the collaborative pulse parameter and sends corresponding high and low voltage pulse control signals to the charging module and the driving circuit respectively. The driving circuit enhances the obtained driving signal to drive the semiconductor switching device in the Marx generator circuit. The Buck circuit reduces the output of the DC charging power supply of the charging module to the low voltage pulse main capacitor group of the Marx generator according to the duty cycle obtained by it, charges the capacitor group, and obtains a low voltage treatment pulse. The Marx generator generates high and low voltage pulse signals with different parameters according to the driving signal and the charging control signal.

2. A high voltage coordinated pulse generating system as claimed in claim 1, wherein: The electrode transmits the high and low voltage collaborative pulses generated by the Marx generator circuit to the load to be treated. Each stage of the Marx circuit is composed of a high-voltage pulse Marx circuit and a low-voltage pulse Marx circuit; wherein the high-voltage pulse Marx circuit is: a high-voltage discharge switch S n1 with the drain connected to the negative pole of the high-voltage pulse circuit isolation diode of the stage, and the source connected to the source of the charging switch S n3 ; a high-voltage pulse main capacitor C n2 with one end connected to the negative pole of the high-voltage pulse circuit isolation diode of the stage, and the other end connected to the drain of the high-voltage charging switch S n3 ; the low-voltage pulse Marx circuit is: a low-voltage discharge switch S n2 with the drain connected to the negative pole of the low-voltage pulse circuit isolation diode of the stage, and the source connected to the source of the charging switch S n3 ; a low-voltage pulse main capacitor C n1 with one end connected to the negative pole of the low-voltage pulse circuit isolation diode of the stage, and the other end connected to the drain of the high-voltage charging switch S n3 ; The Marx generator circuit is an n-stage Marx circuit, where n is an integer greater than or equal to 2; the anode of the high voltage pulse circuit isolation diode in the first stage of the Marx circuit is connected to the anode of the DC charging power supply through a current limiting resistor, the anode of the low voltage pulse circuit isolation diode is connected to the output end of the Buck circuit, and the drain of the high voltage charging switch is connected to the negative electrode of the DC charging power supply; the anode of the high voltage pulse circuit isolation diode in the next stage circuit after the first stage is connected to the negative electrode of the isolation diode in the previous stage, the anode of the low voltage pulse circuit isolation diode is connected to the negative electrode of the low voltage pulse circuit isolation diode in the previous stage, and the drain of the high voltage charging switch is connected to the source of the high voltage charging switch in the previous stage.

3. A high voltage coordinated pulse generating system as claimed in claim 2, wherein: One end of the load is connected to the negative electrode of the DC charging power supply, and the other end is connected to the output of the highest stage of the Marx circuit, i.e. the source of the high / low voltage pulse discharge switch tube in the highest stage.

4. A high voltage coordinated pulse generating system as claimed in claim 2, wherein: The high voltage charging switch, the high voltage discharge switch and the low voltage discharge switch are all fully controlled solid state switch tubes. The power supply U1 passes through the current-limiting resistor R C The Buck circuit reduces the voltage U1 to U2 to charge the low-voltage pulse main capacitor; the high-voltage pulse main capacitor voltage of each stage Marx circuit after charging is completed U 1, the low-voltage pulse main capacitor voltage U 2, U 2=α U 1, α≤1, where α is the duty cycle of the pulse.

5. A high voltage coordinated pulse generating system as claimed in claim 2, wherein: When the system charges the high voltage pulse main capacitor and the low voltage pulse main capacitor, the high voltage discharge switch and the low voltage discharge switch of each stage of the Marx circuit are turned off, and the high voltage charging switch is turned on.

6. A high voltage coordinated pulse generating system as claimed in claim 2, wherein: When the system discharges the high voltage pulse, the high voltage discharge switch of each stage of the Marx circuit is turned on, and the other switches are turned off; the high voltage pulse main capacitors in each stage of the Marx circuit are connected in series to discharge the load, and a high voltage pulse with a voltage of nU1 is obtained, where n is the number of stages of the Marx circuit. When the system discharges the low voltage pulse, the low voltage discharge switch of each stage of the Marx circuit is turned on, and the other switches are turned off; the low voltage pulse main capacitors in each stage of the Marx circuit are connected in series to discharge the load, and a low voltage pulse with a voltage of nU1 is obtained.

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