Sugarless tissue culture system and method for sweet potato

Abstract

The application discloses a kind of sweet potato sugar-free tissue culture systems and methods, it is related to sweet potato culture technical field, including bottom plate, the upper side of the bottom plate is fixedly connected with incubator, fixed frame and carbon dioxide tank, the inside of the incubator is provided with culture inner container, the upper side of the incubator is provided with top cover, the outside of the incubator is provided with processing unit, the processing unit includes two side covers, the side cover is fixedly connected on the left and right sides of incubator, air inlet is formed in the left and right inner walls of the incubator, and the front and rear inner walls of the side cover are fixedly connected with fixed pipe.The sweet potato sugar-free tissue culture system is provided with incubator, culture inner container, carbon dioxide tank and branch pipe, which facilitates the introduction of carbon dioxide into the sweet potato seedlings in the culture inner container, and the uniform assembly facilitates the swing of the rotating fan blades when moving forward and backward, which helps the culture of sweet potato sugar-free tissue.

Description

TECHNICAL FIELD

[0001] The present application relates to sweet potato culture technical field, specifically to a kind of sweet potato sugar-free tissue culture system and method. BACKGROUND

[0002] Sweet potato, also known as red potato, sweet potato, potato, etc., is an important food crop and economic crop, which belongs to perennial herbaceous plant of Convolvulaceae Ipomoea. Sugar-free tissue culture of sweet potato is a culture method using light independent culture technology, which replaces sugar in traditional culture medium by optimizing environmental conditions (such as light, carbon dioxide concentration, ventilation, etc.), to promote photosynthetic autotrophic growth of plant.

[0003] According to the search, the Chinese patent with application number "201911093908.0" is a method for cultivating and transplanting seedlings of virus-free sweet potato by sugar-free culture. The virus-free tissue culture of sweet potato is carried out in a test tube, the virus-free seedlings are proliferated, the old roots of the virus-free seedlings are cut off, the stem segments are cut, and the virus-free seedlings are transplanted into a sugar-free culture box filled with vermiculite, each box is filled with 670g of vermiculite, and 800mL of nutrient solution is poured into each box. The nutrient solution contains 2g / L of calcium nitrate and 170mg / L of potassium dihydrogen phosphate, and the rest is deionized water. Carbon dioxide is introduced into the culture box, and after 25 days of culture in the culture box, the cover is opened, and the seedlings are naturally acclimated and transferred to a greenhouse after 4 days.

[0004] When the above-mentioned virus-free sweet potato seedlings are cultured, carbon dioxide needs to be introduced into the culture box, and the sweet potato seedlings need to be illuminated. The direct introduction of carbon dioxide into the culture box makes the local concentration of carbon dioxide in the culture box too high or too low, which affects the effect of sugar-free tissue culture of sweet potato. The sweet potato seedlings grow unevenly. The light source (such as LED lamp tube) of the culture box is fixed, which causes the light to propagate in a straight line. The light intensity is high in the central area of the culture box and gradually decreases in the edge area. The leaves in the area with too high light intensity are yellow and burned, and the photosynthetic efficiency is reduced. The seedlings in the area with too low light intensity are weak and overgrown (thin and long stems and small leaves), and the dry matter accumulation is less. SUMMARY

[0005] In view of the shortcomings of the prior art, the present application provides a sweet potato sugar-free tissue culture system and method, which solves the problem of uneven distribution of introduced carbon dioxide during sugar-free tissue culture of sweet potato.

[0006] To achieve the above-mentioned purposes, the present application is realized by the following technical solutions:

[0007] The utility model provides a kind of sugarless tissue culture system of sweet potato, including bottom plate, the upper side of the bottom plate is fixedly connected with incubator, fixed frame and carbon dioxide tank, the inside of the incubator is provided with culture inner container, the upper side of the incubator is provided with top cover, the outside of the incubator is provided with processing unit, the processing unit includes two side covers, the side cover is fixedly connected on the left and right sides of incubator, the left and right inner walls of the incubator are all provided with air inlet, the front and rear inner walls of the side cover are fixedly connected with fixed pipe, the outside of the fixed pipe is fixedly connected with multiple branch pipes, the fixed pipe between two is fixedly connected with connecting pipe, the connecting pipe and carbon dioxide tank are fixedly connected with conveying pipe, the inside of the side cover is provided with uniform component, and the uniform component makes that carbon dioxide is evenly distributed.

[0008] Preferably, the uniform component includes a guide plate fixedly connected to the inside of the side cover, a guide block slidingly connected to the outside of the guide plate, two side blocks fixedly connected to the side of the guide block close to the fixed pipe, a rotating shaft rotatably connected between the two side blocks, a rotating block fixedly connected to the outside of the rotating shaft, a fixed frame fixedly connected to the outside of the rotating block, a motor one fixedly connected to one side of the fixed frame, a control shaft rotatably connected to the other side of the fixed frame, a fan blade fixedly connected to the outer end of the control shaft, a bevel gear one fixedly connected to the front end of the rotating shaft passing through the front side of the side block, a limiting block fixedly connected to the front side of the side block, a connecting rod rotatably connected to the outside of the limiting block, a bevel gear two fixedly connected to the lower end of the connecting rod and engaged with the bevel gear one, a gear one fixedly connected to the upper end of the connecting rod, a connecting block one fixedly connected to the upper side of the guide plate, a toothed plate one fixedly connected to the outside of the connecting block one and engaged with the gear one, a reciprocating rod fixedly connected to the rear side of the guide block, a rear plate fixedly connected to the rear end of the reciprocating rod passing through the rear side of the side cover, and a spring fixedly connected between the rear plate and the side cover.

[0009] Preferably, a moving plate is fixedly connected to the rear side of the rear plate, an installation plate two is fixedly connected to the rear side of the fixed frame, a control rod and a rotating shaft are rotatably connected to the front side of the installation plate two, a control plate one is fixedly connected to the front end of the control rod, a control plate two is rotatably connected to the front side of the control plate one, a sliding groove is formed in the front side of the fixed frame, a sliding block one is slidingly connected to the inside of the sliding groove, the sliding block one is rotatably connected with the control plate two, a connecting block two is fixedly connected to the front side of the sliding block one, an inclined plate is rotatably connected to the outside of the connecting block two, a connecting block three is fixedly connected to the rear side of the moving plate, and the connecting block three is rotatably connected with the inclined plate.

[0010] Preferably, a gear two is fixedly connected to the outside of the control rod, a gear three is fixedly connected to the front end of the rotating shaft and engaged with the gear two, and a motor two is fixedly connected to the rear side of the installation plate two to drive the rotating shaft to rotate.

[0011] Preferably, the lower side of the fixing frame is fixedly connected with two bottom blocks, a swing rod is rotatably connected between the two bottom blocks, the outer side of the swing rod is fixedly connected with a swing block, the lower side of the swing block is fixedly connected with a mounting plate one, the lower side of the mounting plate one is fixedly connected with an LED lamp plate, and the rear side of the fixing frame is provided with a swing assembly.

[0012] Preferably, the swing assembly comprises a rotating plate one, the rotating plate one is fixedly connected at the rear end of the control rod, the rear side of the rotating plate one is rotatably connected with a rotating plate two, the rear side of the fixing frame is rotatably connected with a swing shaft, the outer side of the swing shaft is fixedly connected with an arc-shaped block, the rotating plate two is rotatably connected with the arc-shaped block, the rear end of the swing rod penetrates through the rear side of the fixing frame and is fixedly connected with a gear four, the upper side of the arc-shaped block is fixedly connected with a sliding rail, the outer side of the sliding rail is slidably connected with a sliding block two, the outer side of the sliding block two is fixedly connected with a toothed plate two which is meshingly connected with the gear four, the toothed plate two is in an arc-shaped structure, and the upper side of the arc-shaped block and located on the left and right sides of the toothed plate two are both fixedly connected with a stop block.

[0013] A sugar-free tissue culture method of sweet potato, comprising the following steps:

[0014] S1, placing the sweet potato seedling in the culture inner container containing the culture solution, then opening the valve of the carbon dioxide tank to make the plurality of branch pipes spray carbon dioxide into the culture box, and turning on the LED lamp plate to irradiate the sweet potato seedling;

[0015] S2, starting the motor two and the motor one, the motor one drives the fan blade to rotate to blow the carbon dioxide to flow, and the fan blade swings back and forth when repeatedly moving, and the LED lamp plate is controlled to swing intermittently to irradiate the sweet potato seedling;

[0016] S3, recording the growth of the sweet potato seedling.

[0017] Beneficial effects

[0018] The application provides a sugar-free tissue culture system and method of sweet potato.

[0019] (1) The sugar-free tissue culture system of sweet potato, by setting the culture box, the culture inner container, the carbon dioxide tank and the branch pipe, it is convenient to pass into the carbon dioxide to the sweet potato seedling in the culture inner container, and through the uniform assembly, it is convenient for the motor two to control the swing of the rotating fan blade when moving back and forth, so that the carbon dioxide is uniformly distributed in the culture box, which is helpful for the culture of sugar-free tissue of sweet potato.

[0020] (2) The sugar-free tissue culture system of sweet potato, by setting the bottom block, the swing block, the LED lamp plate, the arc-shaped block, the toothed plate two and the gear four, it is convenient to control the swing of the arc-shaped block, so that the LED lamp plate swings intermittently, and the irradiation of the LED lamp plate to the sweet potato seedling is more uniform. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 is a perspective view of the entire structure of the present application;

[0022] Figure 2 is a sectional perspective view of the present application;

[0023] Figure 3 is a partial perspective view of the uniform assembly in the present application;

[0024] Figure 4 is Figure 3 is an enlarged view of A in the middle;

[0025] Figure 5 is a perspective view of the fixed frame in the present application;

[0026] Figure 6 is a perspective view of the fixed frame in the present application;

[0027] Figure 7 is Figure 6 is an enlarged view of B in the middle;

[0028] Figure 8 is Figure 7 is an enlarged view of C in the middle;

[0029] Figure 9 is another perspective view of the fixed frame in the present application;

[0030] Figure 10 is Figure 9 is an enlarged view of D in the middle.

[0031] In the figure: 1, bottom plate; 2, incubator; 3, fixing frame; 4, top cover; 5, carbon dioxide tank; 6, processing unit; 7, connecting pipe; 8, conveying pipe; 9, culture liner; 61, air inlet hole; 62, side cover; 63, fixed pipe; 64, branch pipe; 65, uniform assembly; 66, swing assembly; 67, bottom block; 68, swing rod; 69, swing block; 610, mounting plate one; 611, LED lamp plate; 651, guide plate; 652, guide block; 653, side block; 654, rotating rod; 655, rotating block; 656, gear one; 657, toothed plate one; 658, connecting block one; 659, fixed frame; 6510, motor one; 6511, control shaft; 6512, fan blade; 6513, reciprocating rod; 6514, spring; 6515, back plate; 6516, moving plate; 6517, sliding groove; 6518, sliding block one; 6519, mounting plate two; 6520, control rod; 6521, gear two; 6522, gear three; 6523, rotating shaft; 6524, motor two; 6525, control plate one; 6526, control plate two; 6527, connecting block two; 6528, inclined plate; 6529, connecting block three; 6530, bevel gear one; 6531, bevel gear two; 6532, limiting block; 6533, connecting rod; 661, rotating plate one; 662, rotating plate two; 663, swing shaft; 664, arc-shaped block; 665, slide rail; 666, sliding block two; 667, toothed plate two; 668, gear four; 669, stop block. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by a person of ordinary skill in the art without creative work fall within the protection scope of the present application.

[0033] The present application provides the following technical solutions:

[0034] Embodiment one

[0035] Please refer to Figures 1-8The utility model provides a kind of sugarless tissue culture system of sweet potato, including bottom plate 1, the upper side of bottom plate 1 is fixedly connected with incubator 2, fixed frame 3 and carbon dioxide tank 5, the inside of incubator 2 is provided with culture inner container 9, the upper side of incubator 2 is provided with top cover 4, the outside of incubator 2 is provided with processing unit 6, processing unit 6 includes two side covers 62, side cover 62 is fixedly connected on the left and right sides of incubator 2, the left and right inner walls of incubator 2 are all provided with air inlet 61, fixed pipe 63 is fixedly connected between the front and rear inner walls of side cover 62, a plurality of branch pipes 64 are fixedly connected on the outside of fixed pipe 63, connecting pipe 7 is fixedly connected between two fixed pipes 63, connecting pipe 7 and carbon dioxide tank 5 are fixedly connected with conveying pipe 8, the inside of side cover 62 is provided with uniform component 65, uniform component 65 makes that carbon dioxide is distributed uniformly, wherein valve is installed on carbon dioxide tank 5, the on-off of carbon dioxide is controlled by valve, culture solution and vermiculite are contained in culture inner container 9, when sugarless tissue is cultured, open valve on carbon dioxide tank 5, and carbon dioxide is passed into the fixed pipe 63 of two sides by conveying pipe 8 and connecting pipe 7, and carbon dioxide is passed into incubator 2 by a plurality of branch pipes 64, carbon dioxide is distributed uniformly due to the fact that carbon dioxide is sprayed simultaneously on two sides, and carbon dioxide is distributed more uniformly by uniform component 65, wherein the middle of top cover 4 is provided with rectangular hole, the inside of rectangular hole is provided with polytetrafluoroethylene (PTFE) microporous membrane, the flow of carbon dioxide is facilitated, and external water is blocked, the growth of sweet potato seedling is facilitated.

[0036] The uniform assembly 65 comprises a guide plate 651 fixedly connected to the inner side of the side cover 62, the outer side of the guide plate 651 being slidingly connected with a guide block 652, two side blocks 653 being fixedly connected to the side of the guide block 652 close to the fixed tube 63, a rotating rod 654 being rotatably connected between the two side blocks 653, a rotating block 655 being fixedly connected to the outer side of the rotating rod 654, a fixed frame 659 being fixedly connected to the outer side of the rotating block 655, a motor I 6510 being fixedly connected to one side of the fixed frame 659, a control shaft 6511 being rotatably connected to the other side of the fixed frame 659, a fan blade 6512 being fixedly connected to the outer end of the control shaft 6511, a bevel gear piece I 6530 being fixedly connected to the front end of the rotating rod 654 penetrating through the front side of the side block 653, a limiting block 6532 being fixedly connected to the front side of the side block 653, a connecting rod 6533 being rotatably connected to the outer side of the limiting block 6532, a bevel gear piece II 6531 being fixedly connected to the lower end of the connecting rod 6533 and engaged with the bevel gear piece I 6530, a gear I 656 being fixedly connected to the upper end of the connecting rod 6533, a connecting block I 658 being fixedly connected to the upper side of the guide plate 651, a toothed plate I 657 being fixedly connected to the outer side of the connecting block I 658 and engaged with the gear I 656, a reciprocating rod 6513 being fixedly connected to the rear side of the guide block 652, a rear plate 6515 being fixedly connected to the rear end of the reciprocating rod 6513 penetrating through the rear side of the side cover 62, a spring 6514 being fixedly connected between the rear plate 6515 and the side cover 62, a moving plate 6516 being fixedly connected to the rear side of the rear plate 6515, an installation plate II 6519 being fixedly connected to the rear side of the fixed frame 3, a control rod 6520 and a rotating shaft 6523 being rotatably connected to the front side of the installation plate II 6519, a control plate I 6525 being fixedly connected to the front end of the control rod 6520, a control plate II 6526 being rotatably connected to the front side of the control plate I 6525, a sliding groove 6517 being formed in the front side of the fixed frame 3, a sliding block I 6518 being slidingly connected to the inner side of the sliding groove 6517 and rotatably connected with the control plate II 6526, a connecting block II 6527 being fixedly connected to the front side of the sliding block I 6518, an inclined plate 6528 being rotatably connected to the outer side of the connecting block II 6527, a connecting block III 6529 being fixedly connected to the rear side of the moving plate 6516 and rotatably connected with the inclined plate 6528, a gear II 6521 being fixedly connected to the outer side of the control rod 6520, a gear III 6522 being fixedly connected to the front end of the rotating shaft 6523 and engaged with the gear II 6521, a motor II 6524 being fixedly connected to the rear side of the installation plate II 6519 and driving the rotating shaft 6523 to rotate.

[0037] When carbon dioxide is introduced, the second motor 6524 starts, driving the third gear 6522 on the rotating shaft 6523 to rotate. The third gear 6522 drives the second gear 6521 to rotate, which in turn drives the control lever 6520 to rotate. The control lever 6520 drives the first control plate 6525 to rotate, which in turn drives the second control plate 6526 to rotate. The second control plate 6526 drives the first slider 6518 to move left and right repeatedly. The first slider 6518 drives the inclined plate 6528 on the connecting block 6527 to rotate, which in turn drives the moving plate 6516 to move back and forth repeatedly. The moving plate 6516 drives the rear plate 6515 to move, which in turn drives the reciprocating rod 6513 to move back and forth. The reciprocating rod 6513 drives the guide block 652 to move back and forth. At the same time, the first motor 6510 starts, driving the fan blade 6512 on the control shaft 6511 to rotate. The guide block 652 drives the fan blade 6512 forward and backward. The mechanism moves repeatedly, while the guide block 652 drives the gear 656 to move back and forth repeatedly. Since the gear 656 meshes with the toothed plate 657, the gear 656 rotates. The gear 656 drives the connecting rod 6533 to rotate, the connecting rod 6533 drives the bevel gear 6531 to rotate, the bevel gear 6531 drives the bevel gear 6530 to rotate, the bevel gear 6530 drives the rotating rod 654 to rotate, the rotating rod 654 drives the rotating block 655 to rotate, the rotating block 655 drives the fixed frame 659 to swing, and the fixed frame 659 drives the fan blade 6512 to swing. This allows the fan blade 6512 to move back and forth while swinging, making the emitted carbon dioxide more evenly distributed. Compared with the method of directly introducing carbon dioxide for sugar-free tissue culture, the swing angle of the fan blade 6512 is controlled according to the rotation amplitude of the motor 6524. The space inside the side cover 62 is sufficient for the fixed frame 659 to swing.

[0038] Example 2

[0039] Based on Example 1, such as Figure 9 , Figure 10 As shown, two base blocks 67 are fixedly connected to the lower side of the fixed frame 3. A swing rod 68 is rotatably connected between the two base blocks 67. A swing block 69 is fixedly connected to the outer side of the swing rod 68. A mounting plate 610 is fixedly connected to the lower side of the swing block 69. An LED light panel 611 is fixedly connected to the lower side of the mounting plate 610. A swing assembly 66 is provided on the rear side of the fixed frame 3. When cultivating sweet potato seedlings, the LED light panel 611 is activated to provide light to the sweet potato seedlings. The light and carbon dioxide are introduced simultaneously.

[0040] The swinging assembly 66 comprises a rotating plate one 661 fixedly connected to the rear end of the control rod 6520, a rotating plate two 662 rotatably connected to the rear side of the rotating plate one 661, a swinging shaft 663 rotatably connected to the rear side of the fixed frame 3, an arc-shaped block 664 fixedly connected to the outer side of the swinging shaft 663, the rotating plate two 662 and the arc-shaped block 664 being rotatably connected, the rear end of the swinging rod 68 penetrating through the rear side of the fixed frame 3 and being fixedly connected with a gear four 668, the upper side of the arc-shaped block 664 being fixedly connected with a sliding rail 665, the outer side of the sliding rail 665 being slidably connected with a sliding block two 666, the outer side of the sliding block two 666 being fixedly connected with a toothed plate two 667 in mesh connection with the gear four 668, the toothed plate two 667 being in an arc-shaped structure, the upper side of the arc-shaped block 664 and located on the left and right sides of the toothed plate two 667 being fixedly connected with a stop block 669, when the sweet potato seedlings are irradiated, the motor two 6524 drives the control rod 6520 to rotate, the control rod 6520 drives the rotating plate one 661 to rotate, the rotating plate one 661 drives the rotating plate two 662 to rotate, the rotating plate two 662 drives the arc-shaped block 664 to swing, since the gear four 668 is in mesh connection with the toothed plate two 667, the sliding block two 666 on the toothed plate two 667 slides at the sliding rail 665 (at this time the gear four 668 does not rotate), the arc-shaped block 664 drives the stop block 669 to rotate, when the stop block 669 is in contact with the sliding block two 666, the arc-shaped block 664 continues to rotate, so that the stop block 669 drives the toothed plate two 667 to drive the gear four 668 to rotate, when the arc-shaped block 664 reversely rotates, the gear four 668 does not rotate, when the other stop block 669 drives the toothed plate two 667 to be in contact, the toothed plate two 667 is controlled to rotate, so that the gear four 668 reversely rotates, the intermittent forward and reverse rotation of the gear four 668 is facilitated, the gear four 668 drives the swinging rod 68 to rotate, the swinging rod 68 drives the swinging block 69 to rotate, the swinging block 69 drives the mounting plate one 610 to rotate, the mounting plate one 610 drives the LED lamp plate 611 to swing intermittently, so that the LED lamp plate 611 uniformly irradiates the sweet potato seedlings, and the growth of the sweet potato is facilitated.

[0041] A sugar-free tissue culture method of sweet potato, comprising the following steps:

[0042] S1, placing the sweet potato seedlings in the culture inner container 9 containing the culture solution, then opening the valve of the carbon dioxide tank 5, so that the plurality of branch pipes 64 spray carbon dioxide into the incubator 2, and turning on the LED lamp plate 611 to irradiate the sweet potato seedlings;

[0043] S2, starting the motor two 6524 and the motor one 6510, the motor one 6510 drives the fan blade 6512 to rotate to blow the carbon dioxide to flow, the fan blade 6512 repeatedly moves back and forth to swing, and the LED lamp plate 611 is controlled to swing intermittently to irradiate the sweet potato seedlings;

[0044] S3, recording the growth of the sweet potato seedlings.

[0045] It is to be understood that the terminology used herein such as first and second, and the like, is only used to distinguish one entity or action from another entity or action, and does not necessarily require or imply any such actual relationship or order between such entities or actions. Moreover, the terms "comprises", "comprising", or any other variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can include other elements not expressly listed or inherent to such process, method, article, or apparatus.

[0046] While embodiments of the present application have been shown and described with reference to particular embodiments thereof, it will be understood by those skilled in the art that various changes in form and details can be made therein without departing from the spirit and scope of the application. The scope of the application is defined by the appended claims and their equivalents.

Claims

1. A sugar-free sweet potato tissue culture system, comprising a substrate (1), characterized in that: The upper side of the base plate (1) is fixedly connected to an incubator (2), a fixing frame (3) and a carbon dioxide tank (5). The inner side of the incubator (2) is provided with an inner culture liner (9). The upper side of the incubator (2) is provided with a top cover (4). The outer side of the incubator (2) is provided with a processing unit (6). The processing unit (6) includes two side covers (62). The side covers (62) are fixedly connected to the left and right sides of the incubator (2). The left and right inner walls of the incubator (2) are provided with air inlets (61). The front and rear inner walls of the side covers (62) are fixedly connected with a fixing pipe (63). The outer side of the fixing pipe (63) is fixedly connected with multiple branch pipes (64). The two fixing pipes (63) are fixedly connected with a connecting pipe (7). The connecting pipe (7) and the carbon dioxide tank (5) are fixedly connected with a conveying pipe (8). The inner side of the side cover (62) is provided with a uniform component (65). The uniform component (65) makes the carbon dioxide evenly distributed. The uniform assembly (65) includes a guide plate (651), which is fixedly connected to the inner side of the side cover (62). A guide block (652) is slidably connected to the outer side of the guide plate (651). Two side blocks (653) are fixedly connected to the side of the guide block (652) near the fixed tube (63). A rotating rod (654) is rotatably connected between the two side blocks (653). A rotating block (655) is fixedly connected to the outer side of the rotating rod (654). A fixed frame (659) is fixedly connected to the outer side of the rotating block (655). A motor (6510) is fixedly connected to one side of the fixed frame (659). A control shaft (6511) is rotatably connected to the other side of the fixed frame (659). A fan blade (6512) is fixedly connected to the outer end of the control shaft (6511). The front end of the rotating rod (654) passes through the front side of the side block (653) and is fixedly connected to a bevel gear (6510). 30), a limiting block (6532) is fixedly connected to the front side of the side block (653), a connecting rod (6533) is rotatably connected to the outer side of the limiting block (6532), a bevel gear (6531) is fixedly connected to the lower end of the connecting rod (6533) and meshes with the first bevel gear (6530), a gear (656) is fixedly connected to the upper end of the connecting rod (6533), and the upper side of the guide plate (651) is fixedly connected to There is a connecting block (658), and a toothed plate (657) that meshes with a gear (656) is fixedly connected to the outer side of the connecting block (658). A reciprocating rod (6513) is fixedly connected to the rear side of the guide block (652). The rear end of the reciprocating rod (6513) passes through the rear side of the side cover (62) and is fixedly connected to a rear plate (6515). A spring (6514) is fixedly connected between the rear plate (6515) and the side cover (62). A movable plate (6516) is fixedly connected to the rear side of the rear plate (6515), and a second mounting plate (6519) is fixedly connected to the rear side of the fixed frame (3). A control rod (6520) and a rotating shaft (6523) are rotatably connected to the front side of the second mounting plate (6519). A control plate (6525) is fixedly connected to the front end of the control rod (6520), and a second control plate (6526) is rotatably connected to the front side of the first control plate (6525). A sliding groove (6526) is provided on the front side of the fixed frame (3). 17) A slider 1 (6518) is slidably connected to the inner side of the slide groove (6517). The slider 1 (6518) is rotatably connected to the control plate 2 (6526). A connecting block 2 (6527) is fixedly connected to the front side of the slider 1 (6518). An inclined plate (6528) is rotatably connected to the outer side of the connecting block 2 (6527). A connecting block 3 (6529) is fixedly connected to the rear side of the moving plate (6516). The connecting block 3 (6529) is rotatably connected to the inclined plate (6528). Gear 2 (6521) is fixedly connected to the outside of the control lever (6520), gear 3 (6522) is fixedly connected to the front end of the rotating shaft (6523) and meshes with gear 2 (6521), and motor 2 (6524) that drives the rotating shaft (6523) to rotate is fixedly connected to the rear side of the mounting plate 2 (6519).

2. The sweet potato sugar-free tissue culture system according to claim 1, characterized in that: The lower side of the fixed frame (3) is fixedly connected to two base blocks (67), and a swing rod (68) is rotatably connected between the two base blocks (67). A swing block (69) is fixedly connected to the outer side of the swing rod (68), and a mounting plate (610) is fixedly connected to the lower side of the swing block (69). An LED light board (611) is fixedly connected to the lower side of the mounting plate (610). A swing assembly (66) is provided on the rear side of the fixed frame (3).

3. The sweet potato sugar-free tissue culture system according to claim 2, characterized in that: The swing assembly (66) includes a first rotating plate (661), which is fixedly connected to the rear end of the control rod (6520). A second rotating plate (662) is rotatably connected to the rear side of the first rotating plate (661). A swing shaft (663) is rotatably connected to the rear side of the fixed frame (3). An arc-shaped block (664) is fixedly connected to the outer side of the swing shaft (663). The second rotating plate (662) is rotatably connected to the arc-shaped block (664). The rear end of the swing rod (68) passes through the fixed frame (3). The arc-shaped block (664) is fixedly connected to the rear side of the arc-shaped block (664) and to the upper side of the arc-shaped block (664). A slide rail (665) is fixedly connected to the upper side of the slide rail (665). A slider (666) is slidably connected to the outer side of the slide rail (665). A toothed plate (667) is fixedly connected to the outer side of the slider (666) and meshes with the gear four (668). The toothed plate (667) has an arc-shaped structure. A stop block (669) is fixedly connected to the upper side of the arc-shaped block (664) and to both the left and right sides of the toothed plate (667).

4. A method for sugar-free sweet potato tissue culture, based on the sugar-free sweet potato tissue culture system described in claim 3, characterized in that, Includes the following steps: S1. Place the sweet potato seedlings in the culture tank (9) containing the culture solution, then open the valve of the carbon dioxide tank (5) so that multiple branch pipes (64) spray carbon dioxide into the culture box (2), and turn on the LED light panel (611) to illuminate the sweet potato seedlings. S2. Start motor two (6524) and motor one (6510). Motor one (6510) drives the fan blade (6512) to rotate and blow carbon dioxide. The fan blade (6512) swings back and forth repeatedly, while controlling the LED light panel (611) to swing intermittently to illuminate the sweet potato seedlings. S3. Record the growth of sweet potato seedlings.

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