Composition for promoting intestinal repair and preparation method thereof

Abstract

The invention relates to the technical field of functional food, in particular to a composition for promoting intestinal repair and a preparation method of the composition. 10 to 15 parts of loquat flower leaf extract; 5-10 parts of a dendrobium leaf extract; 5 to 10 parts of glutamine; 3 to 8 parts of fucoidin; 2 to 5 parts of beta-glucan; 1 to 3 parts of probiotics; and 15-25 parts of an auxiliary material. According to the invention, the loquat flower and leaf extract, the dendrobium leaf extract and the fruit pulp matrix are synergistically compounded as a core, and the probiotics and various functional auxiliary materials are supplemented, so that the intestinal repair is realized through a multi-component and multi-mechanism synergistic effect. Therefore, the problems of fuzzy component and efficacy mechanism, poor repair effect, slow improvement process, limited application scene and the like in the prior art are solved.

Description

Technical Field

[0001] This invention relates to the field of functional food technology, specifically to a composition that promotes intestinal repair and its preparation method. Background Technology

[0002] The gut, as a vital digestive and immune organ, is closely related to overall health. The integrity of the intestinal mucosal barrier is fundamental to maintaining normal intestinal function. However, various factors, including poor dietary habits, diseases, medication side effects, and stress, can damage the intestinal mucosa, leading to a range of intestinal diseases such as diarrhea, constipation, and enteritis. Furthermore, gut microbiota imbalance is a significant threat to gut health; the overgrowth of harmful bacteria disrupts the stability of the intestinal environment.

[0003] Currently, there are numerous products on the market for promoting intestinal repair, but most studies focus on the overall efficacy evaluation of traditional Chinese medicine compound formulas, lacking precise functional analysis of different varieties, parts, and graded components, and the depth of research on the material basis is insufficient. Secondly, traditional products suffer from severe loss of active ingredients during extraction, single target sites, and low bioavailability, resulting in long intestinal mucosal repair cycles and low acute inflammation suppression rates, making it difficult to achieve efficient repair. In addition, existing products are mainly in the form of traditional decoction pieces, soups, or drug capsules, which have problems such as poor portability and palatability, insufficient applicability to special populations, and a focus on treatment while neglecting daily maintenance, failing to be optimized for the core needs of modern populations. Summary of the Invention

[0004] This application provides a composition for promoting intestinal repair and a method for preparing the same, in order to solve the problems of unclear components and mechanisms of efficacy, poor repair effect, slow improvement process and limited applicable scenarios in the prior art.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows: The present invention provides a composition for promoting intestinal repair and a method for preparing the same, wherein the composition comprises the following components by weight: Fruit pulp substrate: 40-60 parts; Loquat flower and leaf extract: 10-15 parts; Dendrobium leaf extract: 5-10 parts; Glutamine: 5-10 parts; Fucoidan: 3-8 parts; β-glucan: 2-5 parts; Probiotics: 1-3 servings; Additional ingredients: 15-25 parts.

[0006] Furthermore, the fruit pulp matrix is ​​composed of fructooligosaccharide syrup and loquat pulp in a mass ratio of (1:1) to (2:1).

[0007] Furthermore, the preparation process of the loquat flower and leaf extract includes the following: A1. The dried and pulverized loquat flowers and leaves were extracted three times in sequence as follows; A11. Using 60-90% ethanol solution, with a solid-liquid ratio of 1:30-1:50 g / mL, reflux extraction at 60-80℃ for 1-2 hours. After concentration under reduced pressure, add 1-2 mol / L sodium hydroxide solution to adjust the pH to 9-10, let stand for 10-15 minutes, centrifuge at 4000-6000 r / min for 10-15 minutes, and precipitate as residue. The supernatant contains sodium triterpene acid salt. Add 1-2 mol / L hydrochloric acid solution to the supernatant to adjust the pH to 2-3, let stand at room temperature for 2-3 hours until the triterpene acid is completely precipitated, centrifuge to collect the precipitate, wash with purified water 2-3 times, and vacuum dry to obtain crude triterpene acid extract. A12. Add 40-60% ethanol solution to the residue of step A11, with a material-to-liquid ratio of 1:20-1:30 g / mL, and extract with ultrasonic assistance at 50-70℃ for 1-2 hours. After filtration, concentrate to obtain flavonoid extract. A13. Add pure water to the residue from step A12, with a material-to-liquid ratio of 1:10-1:20 g / mL. Extract at 80-95℃ for 2-3 hours, centrifuge at 4000 r / min for 15 minutes, take the supernatant and concentrate it under reduced pressure to 1 / 10 of the original volume, add 3-4 times the volume of 95% ethanol, let stand at 4℃ for 12 hours, centrifuge to collect the precipitate, and vacuum dry to obtain crude polysaccharide. A2. The crude triterpenic acid extract, the flavonoid extract and the crude polysaccharide are mixed in a mass ratio of (3-5):(2-4):(1-2), and small molecule impurities are removed by ultrafiltration membrane. After freeze-drying, loquat flower and leaf extract is obtained.

[0008] Furthermore, the preparation process of the Dendrobium leaf extract includes the following: B1. The dried and pulverized Dendrobium leaves are processed using a stepwise extraction process combined with compound enzymatic hydrolysis technology; B11. Pulverize dried Dendrobium leaves to 60-80 mesh, add cellulase and pectinase with an enzyme activity ratio of 1:1-1:2, and enzymatically hydrolyze at pH 4.5-5.5 and 45-55℃ for 30-60 minutes. After inactivating the enzyme, filter to obtain the enzymatic hydrolysate. B12. Add 70-90% ethanol solution to the enzymatic hydrolysis residue, with a material-to-liquid ratio of 1:15-1:25 g / mL, reflux at 60-70℃ for 1.5-2.5 hours, centrifuge and concentrate to obtain crude alkaloid extract. B13. Add pure water to the residue of step B12, with a material-to-liquid ratio of 1:20-1:30 g / mL, and extract at 90-100℃ for 3-4 hours. After centrifugation, take the supernatant and concentrate it. Add 3-5 times the volume of 95% ethanol for alcohol precipitation for 12-24 hours, and collect the precipitate to obtain crude polysaccharide. B2. The enzymatic hydrolysate is adsorbed through a macroporous resin column, impurities are first eluted with 20-30% ethanol, and then the target component is eluted with 50-70% ethanol. The eluent is concentrated under reduced pressure and then mixed with the crude alkaloid extract and the crude polysaccharide at a mass ratio of (1-2):(2-3):(4-5). The mixture is then spray-dried to obtain Dendrobium leaf extract.

[0009] Furthermore, the target component is at least one of flavonoids or phenolic acids.

[0010] Furthermore, the probiotics are composed of Bifidobacterium bifidum BB-12 and Bacillus coagulans in a mass ratio of (1:3) to (1:4).

[0011] Furthermore, the viable count of the Bifidobacterium bifidum BB-12 is ≥1×10⁻⁶. 10 CFU / g, the viable count of the Bacillus coagulans is ≥5×10⁻⁶. 9 CFU / g.

[0012] Furthermore, the excipients comprise the following components by weight: Resistant dextrin: 10-20 parts; Sodium bicarbonate: 1-3 parts; Curcumin nanoliposomes: 0.5-2 parts; Natural loquat flavoring: 0.1-0.5 parts.

[0013] Furthermore, the curcumin nanoliposomes have a particle size of 50-200 nm; the resistant dextrin has a degree of polymerization of 10-20 and a water-soluble dietary fiber content of ≥90%.

[0014] Furthermore, it includes the following steps: C1. Weigh the raw materials; C2. Pre-fermentation of fruit pulp substrate: Mix fructooligosaccharide syrup and loquat pulp, heat to 35-40℃, inoculate with Lactobacillus plantarum at an inoculation rate of 3-5%, anaerobic ferment for 12-24 hours, inactivate enzymes at 80℃ for 10 minutes, and cool for later use. C3. Prepare loquat flower and leaf extract and dendrobium leaf extract, and mix fucoidan, β-glucan and resistant dextrin and sodium bicarbonate in the excipients to obtain a premix, and pass it through an 80-100 mesh sieve for later use; C4. Heat the pre-fermented fruit pulp matrix to 35-45℃, add the loquat flower and leaf extract, the dendrobium leaf extract and glutamine in sequence, stir at 200-400 rpm for 20-30 minutes, then add the premix and homogenize it at a pressure of 10-20 MPa for 2-3 cycles. C5. Mix Bifidobacterium bifidum BB-12 with Bacillus coagulans, encapsulate the mixture in microcapsules, and then mix it with the product from step C3 under aseptic conditions with low-speed stirring. The mixture is then dried under low-temperature vacuum at a temperature of 40-50°C and a vacuum degree of -0.08 to -0.1 MPa to obtain a composition. The composition is then dispensed into powder or granules and stored under nitrogen-filled and sealed conditions.

[0015] The beneficial effects achieved by using the present invention described above are as follows: 1. This invention prepares a composition that promotes intestinal repair by synergistically compounding loquat flower and leaf extract, dendrobium leaf extract, and fruit pulp matrix. The loquat flower and leaf extract, after three stages of fractional extraction, yields triterpenoids, flavonoids, and polysaccharides that inhibit iNOS activity and the TLR4 / NF-κB signaling pathway in an LPS-induced RAW264.7 cell model. Simultaneously, it increases the thickness of the intestinal mucus layer by upregulating MUC2 gene expression, forming a dual anti-inflammatory barrier. The dendrobium leaf extract, obtained through a complex enzymatic hydrolysis technique, yields alkaloids, phenolic acids, and polysaccharides that activate the Wnt / β-catenin pathway, increasing Cyclin D1 expression and ZO-1 phosphorylation levels in intestinal epithelial cells, thus promoting intestinal villus regeneration. The fruit pulp matrix, combined with fructooligosaccharide syrup, produces SCFAs through fermentation, which inhibit harmful bacteria, assist in the anti-inflammatory effects of active ingredients, provide colonization sites for probiotics, effectively enhance the TNF-α inhibition rate, and provide a specific carbon source for probiotics.

[0016] 2. This invention incorporates probiotics, wherein Bifidobacterium bifidum BB-12 interacts with mannose receptors on the surface of intestinal epithelial cells via fimbriae protein FimH. 2+ The specific binding of the dependent bacteria enhances colonization efficiency. Bacillus coagulans secretes L-lactic acid with an optical purity >95% through glycolysis, lowering the intestinal pH from 6.8 to 5.2 within 24 hours. This creates an acidic colonization environment for Bifidobacterium bifidum BB-12 and inhibits the growth of harmful bacteria. Simultaneously, the fructooligosaccharide syrup in the fruit pulp matrix is ​​specifically hydrolyzed into fructose-6-phosphate by β-fructosylase secreted by probiotics in the terminal small intestine. This fructose provides energy to the bacterial community at a metabolic rate 1.7 times that of glucose, shortening the logarithmic growth phase of Bifidobacterium bifidum BB-12 to 6 hours and achieving a viable count of 10^6 bacteria within 48 hours. 10With a concentration of CFU / mL, the butyrate produced during fermentation accounts for 42%. It enhances the transcription of anti-inflammatory genes by inhibiting HDAC1, provides 70% of the energy needs of intestinal epithelial cells, and synergistically maintains the intestinal pH at 5.0-5.5 with L-lactic acid. In addition, the "glycoprotein" complex formed by Bifidobacterium bifidum BB-12 and fructooligosaccharide syrup can competitively occupy the adhesion sites of harmful bacteria. The bacteriocins produced by Bacillus coagulans disrupt the cell membrane potential of harmful bacteria, together constituting a dual antibacterial mechanism of "adhesion rejection-membrane damage".

[0017] 3. In addition, a three-layer microcapsule encapsulation technology of sodium alginate-chitosan-maltodextrin is adopted. The microcapsule structure is formed through ion cross-linking-spray drying process. The porosity of its porous wall material reaches 45%, which can not only isolate oxygen to prevent probiotics from oxidative inactivation, but also form a gel barrier in the gastric acid environment. This increases the gastric acid survival rate of Bifidobacterium bifidum BB-12 from 12% in the traditional process to 90%. Moreover, it can be released in a controlled manner under the action of intestinal trypsin, with a live bacteria release rate of 85% within 4 hours, ensuring that the probiotics are accurately colonized in the ileocecal region.

[0018] 4. In the excipients of this invention, sodium bicarbonate forms a dynamic buffer pair with the organic acids in the fruit pulp matrix, releasing CO2 in the stomach to neutralize gastric acid and raising the local pH to a level suitable for protecting probiotics and flavonoids. This reduces the damage of gastric acid to Bifidobacterium bifidum BB-12 and loquat flower and leaf flavonoids, and promotes dosage form disintegration through the microbubble effect, accelerating the release of active ingredients. After entering the intestine, the resistant dextrin is fermented by intestinal flora to produce short-chain fatty acids such as acetic acid and propionic acid, lowering the intestinal pH to 5.0-5.5. On the one hand, this activates the binding ability of the sulfate groups of fucoidan to intestinal mucosal receptors, and on the other hand, it promotes the transmembrane absorption of glutamine through sodium-dependent transporters. Meanwhile, curcumin nanoliposomes form a core-shell structure with lecithin as the membrane material and cholesterol as the stabilizer. The hydrophobic core encapsulates curcumin, and the PEG-modified surface can evade phagocytosis by the reticuloendothelial system. It is delivered to the site of inflamed intestinal tissue via the lymphatic system. Under the action of pH-sensitive lipid membrane, curcumin is released at the site of inflammation. The time to peak blood concentration is shortened from 4-6 hours in traditional formulations to 1.5 hours. It also synergistically inhibits COX-2 enzyme activity with loquat flower and leaf triterpenic acid, enhancing the anti-inflammatory effect. At the same time, the phospholipid bilayer of the nanoliposomes can fuse with intestinal mucosal cells, promoting the endocytic absorption of fat-soluble components such as flavonoids and alkaloids, and improving the overall bioavailability of fat-soluble components.

[0019] 5. In the embodiments of this application, glutamine serves as the main energy source for intestinal mucosal cells, providing 60% of the energy requirement through the TCA cycle, promoting the proliferation of crypt stem cells, and upregulating the expression of tight junction proteins such as ZO-1 to strengthen barrier function. At the same time, it increases the content of secretory IgA by 30% to enhance immunity. Fucoidan uses sulfated fucose residues to mimic the structure of mucin, forming a fibrous gel that increases the mechanical strength of the mucus layer by 40% and inhibits the adhesion rate of harmful bacteria by 70%. The butyrate produced by its fermentation accounts for 38%, which inhibits inflammation through the GPR43 receptor. β-glucan activates the secretion of anti-inflammatory factors through the Dectin-1 receptor, activates the Nrf2 pathway through the CD36 receptor to increase cell survival rate by 60%, and synergistically promotes the formation of probiotic biofilm with fructooligosaccharide syrup, achieving efficient repair of the intestinal mucosa.

[0020] 6. The present invention provides a method for preparing a composition that promotes intestinal repair. The method uses low-temperature vacuum drying at 40-50℃, which reduces the oxidative loss of phenolic acid components in Dendrobium officinale leaves by 42% compared with the traditional hot air drying at 60℃. At the same time, it maintains the structural integrity of the sulfate group of fucoidan (sulfate group retention rate >95%), and increases the extension of its molecular chain in the intestinal mucus layer by 30%, thereby enhancing its binding ability with intestinal mucosal receptors. HPLC detection shows that the heat-sensitive substance retention rate of the active ingredient is 2.3 times higher than that of the traditional process.

[0021] 7. Simultaneously, loquat flowers and leaves were extracted using a three-stage gradient ethanol extraction process. Triterpenic acids were extracted using 60-90% ethanol reflux, flavonoids were extracted using 40-60% ethanol with ultrasound assistance, and crude polysaccharides were obtained by immersion in pure water. This process reduced cross-contamination of components by 37% compared to the traditional decoction method. For dendrobium leaves, a composite enzymatic hydrolysis system of cellulase and pectinase was used. Under conditions of pH 5.0 and 50℃, combined with ultrasonic treatment for 45 minutes, the plant cell wall breakage rate reached 91%, and the phenolic acid dissolution rate increased from 35% to 72%. After the enzymatic hydrolysate was separated by macroporous resin adsorption, the purity of flavonoids and phenolic acids was increased to over 90%. Attached Figure Description

[0022] The above and / or additional aspects and advantages of this application will become apparent and readily understood from the following description of the embodiments taken in conjunction with the accompanying drawings, wherein...

[0023] Figure 1 This is a schematic diagram of an intestinal tissue slice provided in Example 1 of the present invention.

[0024] Figure 2 This is a schematic diagram of an intestinal tissue slice provided in Example 2 of the present invention.

[0025] Figure 3 This is a schematic diagram of an intestinal tissue slice provided in Example 3 of the present invention.

[0026] Figure 4 This is a schematic diagram of an intestinal tissue slice provided in Example 4 of the present invention.

[0027] Figure 5 This is a schematic diagram of an intestinal tissue slice provided in Example 5 of the present invention.

[0028] Figure 6 This is a schematic diagram of the MPO activity provided in the embodiments of the present invention.

[0029] Figure 7 This is a schematic diagram illustrating the relative abundance of various bacilli provided in the embodiments of the present invention.

[0030] Figure 8 This is a schematic diagram showing the comparison of acetic acid content provided in the embodiments of the present invention.

[0031] Figure 9 This is a schematic diagram showing the comparison of propionic acid content in the embodiments of the present invention.

[0032] Figure 10 This is a schematic diagram showing the comparison of butyric acid content in the embodiments of the present invention. Detailed Implementation

[0033] The technical solution of the present invention is illustrated below through specific examples. It should be understood that the one or more method steps mentioned in the present invention do not preclude the existence of other method steps before or after the combined steps, or the insertion of other method steps between these explicitly mentioned steps; it should also be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the present invention. Furthermore, unless otherwise stated, the numbering of each method step is merely a convenient tool for identifying each method step, and not for limiting the order of the method steps or defining the scope of the present invention. Changes or adjustments to their relative relationships, without substantially altering the technical content, should also be considered within the scope of the present invention.

[0034] To better understand the above technical solutions, exemplary embodiments of the present invention are described in more detail below. While exemplary embodiments of the present invention are shown, it should be understood that the present invention can be implemented in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided to enable a more thorough understanding of the present invention and to fully convey the scope of the invention to those skilled in the art.

[0035] The present invention will be further described below with reference to the following embodiments.

[0036] Example 1

[0037] This invention provides a composition for promoting intestinal repair, the composition comprising the following components in parts by weight: Fruit pulp substrate: 40 parts; Loquat flower and leaf extract: 10 parts; Dendrobium leaf extract: 5 parts; Glutamine: 5 parts; Fucoidan: 3 parts; β-glucan: 2 parts; Probiotics: 1 serving; Additional ingredients: 15 portions.

[0038] In the embodiments of this application, the fruit pulp matrix is ​​composed of fructooligosaccharide syrup and loquat fruit pulp in a mass ratio of 1:1.

[0039] In this embodiment of the application, the preparation process of loquat flower and leaf extract includes the following: A1. The dried and pulverized loquat flowers and leaves were extracted three times in sequence as follows; A11. Using 75% ethanol solution, with a solid-liquid ratio of 1:30 g / mL, reflux extraction was performed at 60℃ for 1 hour. After concentration under reduced pressure, 1 mol / L sodium hydroxide solution was added to adjust the pH to 9. The mixture was allowed to stand for 10 minutes, then centrifuged at 4000 r / min for 10 minutes. The precipitate was the residue. The supernatant contained sodium triterpene acid salts. 1 mol / L hydrochloric acid solution was added dropwise to the supernatant to adjust the pH to 2.5. The mixture was allowed to stand at room temperature for 3 hours until the triterpene acid was completely precipitated. The precipitate was collected by centrifugation, washed three times with purified water, and dried under vacuum to obtain the crude triterpene acid extract. A12. Add 40% ethanol solution to the residue of step A11, with a material-to-liquid ratio of 1:20 g / mL, and extract with ultrasonic assistance at 50℃ for 1 hour. After filtration, concentrate to obtain flavonoid extract. A13. Add pure water to the residue of step A12, with a material-to-liquid ratio of 1:10 g / mL, extract at 80℃ for 2 hours, centrifuge at 4000 r / min for 15 minutes, take the supernatant and concentrate it under reduced pressure to 1 / 10 of the original volume, add 3 times the volume of 95% ethanol, let stand at 4℃ for 12 hours, centrifuge to collect the precipitate, and vacuum dry to obtain crude polysaccharide. A2. The crude triterpenic acid extract, flavonoid extract and crude polysaccharide were mixed in a mass ratio of 3:2:1, and small molecule impurities were removed by ultrafiltration membrane. After freeze-drying, loquat flower and leaf extract was obtained.

[0040] In this embodiment of the application, the preparation process of Dendrobium leaf extract includes the following: B1. The dried and pulverized Dendrobium leaves are processed using a stepwise extraction process combined with compound enzymatic hydrolysis technology; B11. Crush dried Dendrobium leaves to 60-80 mesh, add cellulase and pectinase with an enzyme activity ratio of 1:1, and enzymatically hydrolyze at pH 4.5-5.5 and 45-55℃ for 30-60 minutes. After inactivating the enzyme, filter to obtain the enzymatic hydrolysate. B12. Add 70% ethanol solution to the enzymatic hydrolysis residue, with a material-to-liquid ratio of 1:15 g / mL. Reflux at 60℃ for 2 hours. After centrifugation, concentrate to obtain crude alkaloid extract. B13. Add pure water to the residue of step B12 at a material-to-liquid ratio of 1:20 g / mL, extract at 90°C for 3 hours, centrifuge, take the supernatant and concentrate, add 3-5 times the volume of 95% ethanol for 12 hours, collect the precipitate to obtain crude polysaccharide. B2. The enzymatic hydrolysate was adsorbed through a macroporous resin column. Impurities were first eluted with 20% ethanol, and then the target component was eluted with 50% ethanol. The eluent was concentrated under reduced pressure and then mixed with the crude alkaloid extract and crude polysaccharide at a mass ratio of 1:2:4. The mixture was then spray-dried to obtain Dendrobium leaf extract.

[0041] In the embodiments of this application, the target component is at least one of flavonoids or phenolic acids, such as luteolin.

[0042] In the embodiments of this application, the probiotics are composed of Bifidobacterium bifidum BB-12 and Bacillus coagulans in a mass ratio of 1:3.

[0043] In the embodiments of this application, the viable bacterial count of Bifidobacterium bifidum BB-12 is ≥1×10⁻⁶. 10 CFU / g, viable count of Bacillus coagulans ≥5×10 9 CFU / g.

[0044] In this embodiment, the excipients comprise the following components by weight: Resistant dextrin: 10 parts; Sodium bicarbonate: 1 part; Curcumin nanoliposomes: 0.5 parts; Natural loquat flavoring: 0.1 parts.

[0045] In the embodiments of this application, the particle size of curcumin nanoliposomes is 50-200 nm; the degree of polymerization of resistant dextrin is 10-20; and the content of water-soluble dietary fiber is ≥90%.

[0046] In this application embodiment, the following steps are included: C1. Weigh the raw materials; C2. Pre-fermentation of fruit pulp substrate: Mix fructooligosaccharide syrup and loquat pulp, heat to 35°C, inoculate with Lactobacillus plantarum at an inoculation rate of 3%, anaerobic ferment for 12 hours, inactivate enzymes at 80°C for 10 minutes, and cool for later use. C3. Prepare loquat flower and leaf extract and dendrobium leaf extract, and mix fucoidan, β-glucan and resistant dextrin and sodium bicarbonate in the excipients to obtain a premix, and pass it through an 80-100 mesh sieve for later use; C4. Heat the pre-fermented fruit pulp matrix to 35°C, add loquat flower and leaf extract, dendrobium leaf extract and glutamine in sequence, stir at 300 rpm for 20 minutes, then add the premix and homogenize, with a pressure of 10 MPa and 3 cycles. C5. Mix Bifidobacterium bifidum BB-12 with Bacillus coagulans, encapsulate the mixture in microcapsules, and then mix it with the product from step C3 under aseptic conditions with low-speed stirring. The mixture is then dried under low-temperature vacuum at 40°C and a vacuum of -0.08 to -0.1 MPa to obtain the composition. The composition is then dispensed into powder or granules and stored under nitrogen-filled and sealed conditions.

[0047] Example 2

[0048] This invention provides a composition for promoting intestinal repair, the composition comprising the following components in parts by weight: Fruit pulp substrate: 45 parts; Loquat flower and leaf extract: 11 parts; Dendrobium leaf extract: 6 parts; Glutamine: 6 parts; Fucoidan: 4 parts; β-glucan: 3 parts; Probiotics: 1.5 servings; Additional ingredients: 17 portions.

[0049] In the embodiments of this application, the fruit pulp matrix is ​​composed of fructooligosaccharide syrup and loquat fruit pulp in a mass ratio of 1.8:1.

[0050] In this embodiment of the application, the preparation process of loquat flower and leaf extract includes the following: A1. The dried and pulverized loquat flowers and leaves were extracted three times in sequence as follows; A11. Using 75% ethanol solution, with a solid-liquid ratio of 1:35 g / mL, reflux extraction was performed at 70℃ for 1.5 hours. After concentration under reduced pressure, 1.2 mol / L sodium hydroxide solution was added to adjust the pH to 9. The mixture was allowed to stand for 12 minutes, then centrifuged at 4500 r / min for 12 minutes. The precipitate was the residue. The supernatant contained sodium triterpene acid salts. 2 mol / L hydrochloric acid solution was added dropwise to the supernatant to adjust the pH to 3. The mixture was allowed to stand at room temperature for 3 hours until the triterpene acid was completely precipitated. The precipitate was collected by centrifugation, washed three times with purified water, and dried under vacuum to obtain the crude triterpene acid extract. A12. Add 50% ethanol solution to the residue of step A11, with a material-to-liquid ratio of 1:23 g / mL, and extract with ultrasonic assistance at 60℃ for 2 hours. After filtration, concentrate to obtain flavonoid extract. A13. Add pure water to the residue from step A12 at a ratio of 1:13 g / mL, extract at 90°C for 3 hours, centrifuge at 4000 r / min for 15 minutes, take the supernatant and concentrate it under reduced pressure to 1 / 10 of the original volume, add 3.5 times the volume of 95% ethanol, let stand at 4°C for 12 hours, centrifuge to collect the precipitate, and vacuum dry to obtain crude polysaccharide. A2. The crude triterpenic acid extract, flavonoid extract and crude polysaccharide were mixed in a mass ratio of 3.5:2.5:1.3, and small molecule impurities were removed by ultrafiltration membrane. After freeze-drying, loquat flower and leaf extract was obtained.

[0051] In this embodiment of the application, the preparation process of Dendrobium leaf extract includes the following: B1. The dried and pulverized Dendrobium leaves are processed using a stepwise extraction process combined with compound enzymatic hydrolysis technology; B11. Pulverize dried Dendrobium leaves to 60-80 mesh, add cellulase and pectinase with an enzyme activity ratio of 1:1.3, and enzymatically hydrolyze for 40 minutes at pH 4.5-5.5 and 45-55℃. After inactivating the enzyme, filter to obtain the enzymatic hydrolysate. B12. Add 80% ethanol solution to the enzymatic hydrolysis residue, with a material-to-liquid ratio of 1:18 g / mL. Reflux at 70°C for 2 hours. After centrifugation, concentrate to obtain crude alkaloid extract. B13. Add pure water to the residue of step B12, with a material-to-liquid ratio of 1:23 g / mL, extract at 100℃ for 4 hours, centrifuge, take the supernatant and concentrate, add 3-5 times the volume of 95% ethanol for 24 hours, collect the precipitate to obtain crude polysaccharide. B2. The enzymatic hydrolysate was adsorbed through a macroporous resin column. Impurities were first eluted with 30% ethanol, and then the target component was eluted with 60% ethanol. The eluent was concentrated under reduced pressure and then mixed with crude alkaloid extract and crude polysaccharide at a mass ratio of 1.3:2.3:4.3. The mixture was then spray-dried to obtain Dendrobium leaf extract.

[0052] In the embodiments of this application, the target component is at least one of flavonoids or phenolic acids, such as kaempferol.

[0053] In the embodiments of this application, the probiotics are composed of Bifidobacterium bifidum BB-12 and Bacillus coagulans in a mass ratio of 1:3.3.

[0054] In the embodiments of this application, the viable bacterial count of Bifidobacterium bifidum BB-12 is ≥1×10⁻⁶. 10 CFU / g, viable count of Bacillus coagulans ≥5×10 9 CFU / g.

[0055] In this embodiment, the excipients comprise the following components by weight: Resistant dextrin: 13 parts; Sodium bicarbonate: 1.5 parts; Curcumin nanoliposomes: 1 part; Natural loquat flavoring: 0.2 parts.

[0056] In the embodiments of this application, the particle size of curcumin nanoliposomes is 50-200 nm; the degree of polymerization of resistant dextrin is 10-20; and the content of water-soluble dietary fiber is ≥90%.

[0057] In this application embodiment, the following steps are included: C1. Weigh the raw materials; C2. Pre-fermentation of fruit pulp substrate: Mix fructooligosaccharide syrup and loquat pulp, heat to 36°C, inoculate with Lactobacillus plantarum at an inoculation rate of 4%, anaerobic ferment for 20 hours, inactivate enzymes at 80°C for 10 minutes, and cool for later use. C3. Prepare loquat flower and leaf extract and dendrobium leaf extract, and mix fucoidan, β-glucan and resistant dextrin and sodium bicarbonate in the excipients to obtain a premix, and pass it through an 80-100 mesh sieve for later use; C4. Heat the pre-fermented fruit pulp matrix to 38°C, add loquat flower and leaf extract, dendrobium leaf extract and glutamine in sequence, stir at 400 rpm for 30 minutes, then add the premix and homogenize, with a pressure of 12 MPa and 3 cycles. C5. Mix Bifidobacterium bifidum BB-12 with Bacillus coagulans, encapsulate the mixture in microcapsules, and then mix it with the product from step C3 under aseptic conditions with low-speed stirring. The mixture is then dried under low-temperature vacuum at 50°C and a vacuum of -0.08 to -0.1 MPa to obtain the composition. The composition is then dispensed into powder or granules and stored under nitrogen-filled and sealed conditions.

[0058] Example 3

[0059] This invention provides a composition for promoting intestinal repair, the composition comprising the following components in parts by weight: Fruit pulp substrate: 50 parts; Loquat flower and leaf extract: 12.5 parts; Dendrobium leaf extract: 7.5 parts; Glutamine: 7.5 parts; Fucoidan: 5.5 parts; β-glucan: 3.5 parts; Probiotics: 2 servings; Additional ingredients: 20 portions.

[0060] In the embodiments of this application, the fruit pulp matrix is ​​composed of fructooligosaccharide syrup and loquat fruit pulp in a mass ratio of 1.5:1.

[0061] In this embodiment of the application, the preparation process of loquat flower and leaf extract includes the following: A1. The dried and pulverized loquat flowers and leaves were extracted three times in sequence as follows; A11. Using 90% ethanol solution, with a solid-liquid ratio of 1:40 g / mL, reflux extraction at 80℃ for 2 hours. After concentration under reduced pressure, 1.5 mol / L sodium hydroxide solution was added to adjust the pH to 10. After standing for 15 minutes, centrifugation was performed at 5000 r / min for 15 minutes, and the precipitate was the residue. The supernatant contained sodium triterpene acid salt. 2 mol / L hydrochloric acid solution was added dropwise to the supernatant to adjust the pH to 3. After standing at room temperature for 3 hours, the triterpene acid was completely precipitated. The precipitate was collected by centrifugation, washed 3 times with purified water, and vacuum dried to obtain crude triterpene acid extract. A12. Add 60% ethanol solution to the residue of step A11, with a material-to-liquid ratio of 1:25 g / mL, and extract with ultrasonic assistance at 70℃ for 2 hours. After filtration, concentrate to obtain flavonoid extract. A13. Add pure water to the residue of step A12, with a material-to-liquid ratio of 1:15 g / mL, extract at 95°C for 3 hours, centrifuge at 4000 r / min for 15 minutes, take the supernatant and concentrate it under reduced pressure to 1 / 10 of the original volume, add 4 times the volume of 95% ethanol, let stand at 4°C for 12 hours, centrifuge to collect the precipitate, and vacuum dry to obtain crude polysaccharide. A2. The crude triterpenic acid extract, flavonoid extract and crude polysaccharide were mixed in a mass ratio of 4:3:1.5, and small molecule impurities were removed by ultrafiltration membrane. After freeze-drying, loquat flower and leaf extract was obtained.

[0062] In this embodiment of the application, the preparation process of Dendrobium leaf extract includes the following: B1. The dried and pulverized Dendrobium leaves are processed using a stepwise extraction process combined with compound enzymatic hydrolysis technology; B11. Pulverize dried Dendrobium leaves to 60-80 mesh, add cellulase and pectinase with an enzyme activity ratio of 1:1.5, and enzymatically hydrolyze for 60 minutes at pH 4.5-5.5 and 45-55℃. After inactivating the enzyme, filter to obtain the enzymatic hydrolysate. B12. Add 80% ethanol solution to the enzymatic hydrolysis residue at a material-to-liquid ratio of 1:20 g / mL, reflux at 70°C for 2.5 hours, centrifuge and concentrate to obtain crude alkaloid extract. B13. Add pure water to the residue of step B12, with a material-to-liquid ratio of 1:25 g / mL, extract at 100℃ for 4 hours, centrifuge, take the supernatant and concentrate, add 3-5 times the volume of 95% ethanol for 24 hours, collect the precipitate to obtain crude polysaccharide. B2. The enzymatic hydrolysate was adsorbed through a macroporous resin column. Impurities were first eluted with 30% ethanol, and then the target component was eluted with 60% ethanol. The eluent was concentrated under reduced pressure and then mixed with crude alkaloid extract and crude polysaccharide at a mass ratio of 1.5:2.5:4.5. The mixture was then spray-dried to obtain Dendrobium leaf extract.

[0063] In the embodiments of this application, the target component is at least one of flavonoids or phenolic acids, such as quercetin.

[0064] In the embodiments of this application, the probiotics are composed of Bifidobacterium bifidum BB-12 and Bacillus coagulans in a mass ratio of 1:3.5.

[0065] In the embodiments of this application, the viable bacterial count of Bifidobacterium bifidum BB-12 is ≥1×10⁻⁶. 10 CFU / g, viable count of Bacillus coagulans ≥5×10 9 CFU / g.

[0066] In this embodiment, the excipients comprise the following components by weight: Resistant dextrin: 15 parts; Sodium bicarbonate: 2 parts; Curcumin nanoliposomes: 1.3 parts; Natural loquat flavoring: 0.3 parts.

[0067] In the embodiments of this application, the particle size of curcumin nanoliposomes is 50-200 nm; the degree of polymerization of resistant dextrin is 10-20; and the content of water-soluble dietary fiber is ≥90%.

[0068] In this application embodiment, the following steps are included: C1. Weigh the raw materials; C2. Pre-fermentation of fruit pulp substrate: Mix fructooligosaccharide syrup and loquat pulp, heat to 38°C, inoculate with Lactobacillus plantarum at an inoculation rate of 5%, anaerobic ferment for 20 hours, inactivate enzymes at 80°C for 10 minutes, and cool for later use. C3. Prepare loquat flower and leaf extract and dendrobium leaf extract, and mix fucoidan, β-glucan and resistant dextrin and sodium bicarbonate in the excipients to obtain a premix, and pass it through an 80-100 mesh sieve for later use; C4. Heat the pre-fermented fruit pulp matrix to 45°C, add loquat flower and leaf extract, dendrobium leaf extract and glutamine in sequence, stir at 400 rpm for 30 minutes, then add the premixed material and homogenize it at a pressure of 15 MPa for 3 cycles. C5. Mix Bifidobacterium bifidum BB-12 with Bacillus coagulans, encapsulate the mixture in microcapsules, and then mix it with the product from step C3 under aseptic conditions with low-speed stirring. The mixture is then dried under low-temperature vacuum at 50°C and a vacuum of -0.08 to -0.1 MPa to obtain the composition. The composition is then dispensed into powder or granules and stored under nitrogen-filled and sealed conditions.

[0069] Example 4

[0070] This invention provides a composition for promoting intestinal repair, the composition comprising the following components in parts by weight: Fruit pulp substrate: 55 parts; Loquat flower and leaf extract: 14 parts; Dendrobium leaf extract: 9 parts; Glutamine: 9 parts; Fucoidan: 7 parts; β-glucan: 4 parts; Probiotics: 2.5 servings; Additional ingredients: 23 portions.

[0071] In the embodiments of this application, the fruit pulp matrix is ​​composed of fructooligosaccharide syrup and loquat fruit pulp in a mass ratio of 1.2:1.

[0072] In this embodiment of the application, the preparation process of loquat flower and leaf extract includes the following: A1. The dried and pulverized loquat flowers and leaves were extracted three times in sequence as follows; A11. Using 90% ethanol solution, with a solid-liquid ratio of 1:45 g / mL, reflux extraction at 80℃ for 1-2 hours. After concentration under reduced pressure, add 2 mol / L sodium hydroxide solution to adjust the pH to 10, let stand for 15 minutes, centrifuge at 5500 r / min for 15 minutes, and the precipitate is the residue. The supernatant contains sodium triterpene acid salt. Add 2 mol / L hydrochloric acid solution to the supernatant to adjust the pH to 3, let stand at room temperature for 3 hours until the triterpene acid is completely precipitated, centrifuge to collect the precipitate, wash 3 times with purified water, and vacuum dry to obtain crude triterpene acid extract. A12. Add 60% ethanol solution to the residue of step A11, with a material-to-liquid ratio of 1:28 g / mL, and extract with ultrasonic assistance at 70℃ for 2 hours. After filtration, concentrate to obtain flavonoid extract. A13. Add pure water to the residue of step A12, with a material-to-liquid ratio of 1:18 g / mL, extract at 95℃ for 3 hours, centrifuge at 4000 r / min for 15 minutes, take the supernatant and concentrate it under reduced pressure to 1 / 10 of the original volume, add 4 times the volume of 95% ethanol, let stand at 4℃ for 12 hours, centrifuge to collect the precipitate, and vacuum dry to obtain crude polysaccharide. A2. The crude triterpenic acid extract, flavonoid extract and crude polysaccharide were mixed in a mass ratio of 4.5:3.5:1.8, and small molecule impurities were removed by ultrafiltration membrane. After freeze-drying, loquat flower and leaf extract was obtained.

[0073] In this embodiment of the application, the preparation process of Dendrobium leaf extract includes the following: B1. The dried and pulverized Dendrobium leaves are processed using a stepwise extraction process combined with compound enzymatic hydrolysis technology; B11. Pulverize dried Dendrobium leaves to 60-80 mesh, add cellulase and pectinase with an enzyme activity ratio of 1:1.8, and enzymatically hydrolyze for 60 minutes at pH 4.5-5.5 and 45-55℃. After inactivating the enzyme, filter to obtain the enzymatic hydrolysate. B12. Add 90% ethanol solution to the enzymatic hydrolysis residue at a material-to-liquid ratio of 1:23 g / mL, reflux at 70°C for 2.5 hours, centrifuge and concentrate to obtain crude alkaloid extract. B13. Add pure water to the residue of step B12, with a material-to-liquid ratio of 1:28 g / mL, extract at 100℃ for 4 hours, centrifuge, take the supernatant and concentrate, add 3-5 times the volume of 95% ethanol for 24 hours, collect the precipitate to obtain crude polysaccharide. B2. The enzymatic hydrolysate was adsorbed through a macroporous resin column. Impurities were first eluted with 30% ethanol, and then the target component was eluted with 70% ethanol. The eluent was concentrated under reduced pressure and then mixed with crude alkaloid extract and crude polysaccharide at a mass ratio of 1.8:2.8:4.8. The mixture was then spray-dried to obtain Dendrobium leaf extract.

[0074] In the embodiments of this application, the target component is at least one of flavonoids or phenolic acids, such as luteolin and quercetin.

[0075] In the embodiments of this application, the probiotics are composed of Bifidobacterium bifidum BB-12 and Bacillus coagulans in a mass ratio of 1:3.8.

[0076] In the embodiments of this application, the viable bacterial count of Bifidobacterium bifidum BB-12 is ≥1×10⁻⁶. 10 CFU / g, viable count of Bacillus coagulans ≥5×10 9 CFU / g.

[0077] In this embodiment, the excipients comprise the following components by weight: Resistant dextrin: 18 parts; Sodium bicarbonate: 2.5 parts; Curcumin nanoliposomes: 1.5 parts; Natural loquat flavoring: 0.4 parts.

[0078] In the embodiments of this application, the particle size of curcumin nanoliposomes is 50-200 nm; the degree of polymerization of resistant dextrin is 10-20; and the content of water-soluble dietary fiber is ≥90%.

[0079] In this application embodiment, the following steps are included: C1. Weigh the raw materials; C2. Pre-fermentation of fruit pulp substrate: Mix fructooligosaccharide syrup and loquat pulp, heat to 39°C, inoculate with Lactobacillus plantarum at an inoculation rate of 5%, anaerobic ferment for 24 hours, inactivate enzymes at 80°C for 10 minutes, and cool for later use. C3. Prepare loquat flower and leaf extract and dendrobium leaf extract, and mix fucoidan, β-glucan and resistant dextrin and sodium bicarbonate in the excipients to obtain a premix, and pass it through an 80-100 mesh sieve for later use; C4. Heat the pre-fermented fruit pulp matrix to 45°C, add loquat flower and leaf extract, dendrobium leaf extract and glutamine in sequence, stir at 400 rpm for 30 minutes, then add the premix and homogenize, with a pressure of 18 MPa and 3 cycles. C5. Mix Bifidobacterium bifidum BB-12 with Bacillus coagulans, encapsulate the mixture in microcapsules, and then mix it with the product from step C3 under aseptic conditions with low-speed stirring. The mixture is then dried under low-temperature vacuum at 50°C and a vacuum of -0.08 to -0.1 MPa to obtain the composition. The composition is then dispensed into powder or granules and stored under nitrogen-filled and sealed conditions.

[0080] Example 5

[0081] This invention provides a composition for promoting intestinal repair, the composition comprising the following components in parts by weight: Fruit pulp substrate: 60 parts; Loquat flower and leaf extract: 15 parts; Dendrobium leaf extract: 10 parts; Glutamine: 10 parts; Fucoidan: 8 parts; β-glucan: 5 parts; Probiotics: 3 servings; Additional ingredients: 25 portions.

[0082] In the embodiments of this application, the fruit pulp matrix is ​​composed of fructooligosaccharide syrup and loquat fruit pulp in a mass ratio of 1:1.

[0083] In this embodiment of the application, the preparation process of loquat flower and leaf extract includes the following: A1. The dried and pulverized loquat flowers and leaves were extracted three times in sequence as follows; A11. Using 90% ethanol solution at a material-to-liquid ratio of 1:50 g / mL, reflux extraction was performed at 80℃ for 2 hours. After concentration under reduced pressure, 2 mol / L sodium hydroxide solution was added to adjust the pH to 10. The mixture was allowed to stand for 15 minutes, then centrifuged at 6000 r / min for 15 minutes. The precipitate was the residue. The supernatant contained sodium triterpene acid. 2 mol / L hydrochloric acid solution was added dropwise to the supernatant to adjust the pH to 3. The mixture was allowed to stand at room temperature for 3 hours until the triterpene acid was completely precipitated. The precipitate was collected by centrifugation, washed three times with purified water, and dried under vacuum to obtain the crude triterpene acid extract. A12. Add 60% ethanol solution to the residue of step A11, with a material-to-liquid ratio of 1:30 g / mL, and extract with ultrasonic assistance at 70℃ for 2 hours. After filtration, concentrate to obtain flavonoid extract. A13. Add pure water to the residue of step A12, with a material-to-liquid ratio of 1:20 g / mL, extract at 95°C for 3 hours, centrifuge at 4000 r / min for 15 minutes, take the supernatant and concentrate it under reduced pressure to 1 / 10 of the original volume, add 4 times the volume of 95% ethanol, let stand at 4°C for 12 hours, centrifuge to collect the precipitate, and vacuum dry to obtain crude polysaccharide. A2. The crude triterpenic acid extract, flavonoid extract and crude polysaccharide were mixed in a mass ratio of 5:4:2, and small molecule impurities were removed by ultrafiltration membrane. After freeze-drying, loquat flower and leaf extract was obtained.

[0084] In this embodiment of the application, the preparation process of Dendrobium leaf extract includes the following: B1. The dried and pulverized Dendrobium leaves are processed using a stepwise extraction process combined with compound enzymatic hydrolysis technology; B11. Pulverize dried Dendrobium leaves to 60-80 mesh, add cellulase and pectinase with an enzyme activity ratio of 1:2, and enzymatically hydrolyze for 60 minutes at pH 4.5-5.5 and 45-55℃. After inactivating the enzyme, filter to obtain the enzymatic hydrolysate. B12. Add 90% ethanol solution to the enzymatic hydrolysis residue at a material-to-liquid ratio of 1:25 g / mL, reflux at 70°C for 2.5 hours, centrifuge and concentrate to obtain crude alkaloid extract. B13. Add pure water to the residue of step B12, with a material-to-liquid ratio of 1:30 g / mL, extract at 100℃ for 4 hours, centrifuge, take the supernatant and concentrate, add 3-5 times the volume of 95% ethanol for 24 hours, collect the precipitate to obtain crude polysaccharide. B2. The enzymatic hydrolysate was adsorbed through a macroporous resin column. Impurities were first eluted with 30% ethanol, and then the target component was eluted with 70% ethanol. The eluent was concentrated under reduced pressure and then mixed with crude alkaloid extract and crude polysaccharide at a mass ratio of 2:3:5. The mixture was then spray-dried to obtain Dendrobium leaf extract.

[0085] In the embodiments of this application, the target component is at least one of flavonoids or phenolic acids, such as luteolin and kaempferol.

[0086] In the embodiments of this application, the probiotics are composed of Bifidobacterium bifidum BB-12 and Bacillus coagulans in a mass ratio of 1:4.

[0087] In the embodiments of this application, the viable bacterial count of Bifidobacterium bifidum BB-12 is ≥1×10⁻⁶. 10 CFU / g, viable count of Bacillus coagulans ≥5×10 9 CFU / g.

[0088] In this embodiment, the excipients comprise the following components by weight: Resistant dextrin: 20 parts; Sodium bicarbonate: 3 parts; Curcumin nanoliposomes: 2 parts; Natural loquat flavoring: 0.5 parts.

[0089] In the embodiments of this application, the particle size of curcumin nanoliposomes is 50-200 nm; the degree of polymerization of resistant dextrin is 10-20; and the content of water-soluble dietary fiber is ≥90%.

[0090] In this application embodiment, the following steps are included: C1. Weigh the raw materials; C2. Pre-fermentation of fruit pulp substrate: Mix fructooligosaccharide syrup and loquat pulp, heat to 40°C, inoculate with Lactobacillus plantarum at an inoculation rate of 5%, anaerobic ferment for 24 hours, inactivate enzymes at 80°C for 10 minutes, and cool for later use. C3. Prepare loquat flower and leaf extract and dendrobium leaf extract, and mix fucoidan, β-glucan and resistant dextrin and sodium bicarbonate in the excipients to obtain a premix, and pass it through an 80-100 mesh sieve for later use; C4. Heat the pre-fermented fruit pulp matrix to 45°C, add loquat flower and leaf extract, dendrobium leaf extract and glutamine in sequence, stir at 400 rpm for 30 minutes, then add the premix and homogenize, with a pressure of 20 MPa and 3 cycles. C5. Mix Bifidobacterium bifidum BB-12 with Bacillus coagulans, encapsulate the mixture in microcapsules, and then mix it with the product from step C3 under aseptic conditions with low-speed stirring. The mixture is then dried under low-temperature vacuum at 50°C and a vacuum of -0.08 to -0.1 MPa to obtain the composition. The composition is then dispensed into powder or granules and stored under nitrogen-filled and sealed conditions.

[0091] Comparative Example 1 A composition for promoting intestinal repair, which differs from Example 1 only in the absence of loquat flower and leaf extract, the reduced amount of loquat flower and leaf extract being allocated to excipients, while the other components and preparation method are the same.

[0092] Comparative Example 2 A composition for promoting intestinal repair, which differs from Example 1 only in the absence of Dendrobium leaf extract, with the reduced amount of Dendrobium leaf extract allocated to excipients, while the other components and preparation method are the same.

[0093] Comparative Example 3 A composition for promoting intestinal repair differs from Example 1 only in that it does not contain ungraded loquat flower and leaf extract. The loquat flower and leaf extract is obtained by traditional decoction method, wherein the water-to-material ratio is 1:30, and the decoction is decocted twice at 100°C for 1 hour each time. The decoction is then combined, concentrated, and freeze-dried. The remaining processes are the same as in Example 1.

[0094] Comparative Example 4 A composition for promoting intestinal repair differs from Example 1 only in that it does not contain Dendrobium leaf extract that has not been enzymatically hydrolyzed. The enzymatic hydrolysis step of Dendrobium leaves is omitted, and it is directly extracted by reflux with 70% ethanol solution (material-liquid ratio 1:15) for 2 hours. The rest of the process is the same as in Example 1.

[0095] Comparative Example 5 A composition for promoting intestinal repair, which differs from Example 1 only in that it lacks Dendrobium leaf extract and loquat flower and leaf extract. The reduced amount of Dendrobium leaf extract and loquat flower and leaf extract is allocated to the excipients. The other components and preparation method are the same.

[0096] Comparative Example 6 A composition for promoting intestinal repair, which differs from Example 1 only in the absence of probiotics, with the reduced amount of probiotics allocated to excipients, while the other components and preparation method are the same.

[0097] Comparative Example 7 A composition for promoting intestinal repair, which differs from Example 1 only in that it contains loquat flower and leaf extract, with the remaining components replaced by excipients, and is prepared using the same method.

[0098] Comparative Example 8 A composition for promoting intestinal repair, which differs from Example 1 in that it contains only Dendrobium leaf extract, with the remaining components replaced by excipients, and is prepared using the same method.

[0099] Comparative Example 9 A composition for promoting intestinal repair, which differs from Example 1 in that it contains only loquat flower and leaf extract and dendrobium leaf extract, but does not contain fruit pulp matrix, and the remaining components are replaced with excipients, and the preparation method is the same.

[0100] Comparative Example 10 A composition for promoting intestinal repair, which differs from Embodiment 1 only in that the probiotics are encapsulated in a single layer of sodium alginate, while the other components and preparation method are the same. Performance testing

[0101] An acute enteritis model was induced in C57BL / 6 mice using DSS (2.5%). Seven days after modeling, mice exhibiting diarrhea and bloody stools were selected for experiments.

[0102] Group processing: 13 groups in total, 10 animals in each group: Normal group: administered physiological saline by gavage; Model group: administered physiological saline by gavage; Examples 1-5: The corresponding composition (0.2g / kg) was administered by gavage respectively. Comparative Examples 1-10: The corresponding formulation composition (0.2g / kg) was administered by gavage.

[0103] Administration cycle: Administer by gavage for 14 consecutive days, and record mouse body weight, diarrhea index and bloody stools daily.

[0104] The detection indicators and results are as follows: The pathological observation of intestinal tissue is shown in Table 1 below.

[0105] Table 1. Pathological observation of intestinal tissue

[0106] As shown in Table 1, this experiment constructed a DSS-induced acute enteritis model in mice and compared the intestinal repair effects of Examples 1-5 and the 10 comparative groups. The results showed that all examples significantly improved intestinal villus height, crypt depth, and inflammatory cell infiltration. Example 5 showed the best repair effect, restoring intestinal villus height to the level of the normal group and reducing the inflammation score to 0.9±0.2. Comparative verification showed that the lack of loquat flower and leaf extract or dendrobium leaf extract decreased the repair efficiency, and traditional extraction processes and the absence of encapsulated probiotics also led to reduced efficacy.

[0107] The barrier function indicators are shown in Table 2 below.

[0108] Table 2 Barrier Function Indicators Detection

[0109] As shown in Table 2, this experiment evaluated the intestinal mucosal barrier repair effects of Examples 1-5 and the comparative examples by detecting the expression of intestinal tight junction proteins ZO-1 and Occludin. The results showed that all examples significantly improved the levels of both indicators. In Example 5, ZO-1 and Occludin recovered to 93.8% and 97.2% of the normal group, respectively, approaching healthy levels. Comparative analysis indicated that the lack of loquat flower and leaf extracts or dendrobium leaf extracts reduced the barrier function repair efficiency by 35%-51%, and traditional extraction processes and the absence of probiotic encapsulation also led to a reduction in effectiveness of 18%-39%.

[0110] The inflammatory factors were detected, as shown in Table 3 below.

[0111] Table 3. Detection of inflammatory factors

[0112] From Table 3, Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5As shown, this experiment evaluated the inhibitory effects of Examples 1-5 and the comparative examples on intestinal inflammation by detecting the levels of three inflammatory factors: TNF-α, IL-6, and IL-1β. The results showed that the levels of inflammatory factors in the model group were significantly higher than those in the normal group, confirming the successful construction of the acute enteritis model. All examples effectively reduced the content of inflammatory factors. Among them, Example 5 showed the best effect, with TNF-α, IL-6, and IL-1β levels decreasing to 24.9±2.7 pg / mL, 22.5±2.2 pg / mL, and 19.8±1.7 pg / mL, respectively, with inhibition rates of 72.2%, 68.8%, and 69.5%. Due to component deficiencies or process defects, the levels of inflammatory factors in the comparative examples were significantly higher than those in the example groups. The levels of inflammatory factors in Comparative Examples 7 and 8 were significantly higher than those in Example 5, confirming the limited effectiveness of single-component formulations. The effect of Comparative Example 9 was better than that of single-component formulations but still weaker than that of Example 5, verifying the necessity of the synergistic anti-inflammatory effect of "Loquat flower and leaf + Dendrobium leaf + fruit pulp matrix".

[0113] like Figure 6 As shown, the MPO activity in the normal group was 0.32±0.05 U / g, which is at a low level and represents a normal physiological state. The MPO activity in the model group was as high as 2.15±0.18 U / g, significantly higher than that in the normal group, indicating a strong inflammatory response. The MPO activities in Examples 1-5 ranged from 0.60±0.05 to 0.68±0.07 U / g, significantly lower than that in the model group and showing a decreasing trend, indicating that the treatment methods in these examples can effectively inhibit inflammation, reduce MPO activity, and the effect gradually increases. The MPO activities in Comparative Examples 1-6 ranged from 0.79±0.06 to 1.23±0.12 U / g, which were lower than that in the model group but higher than those in the Example groups, indicating that their anti-inflammatory effect was not as good as that in the Example groups.

[0114] like Figure 7As shown, the normal group had a high proportion of Bifidobacteria and Lactobacillus, at 18.7±2.1% and 12.5±1.8% respectively, while the proportion of Escherichia coli was low (3.2±0.5%), and the Shannon index reached 3.85±0.21, indicating a rich and balanced microbial community. In the model group, the proportions of Bifidobacteria and Lactobacillus decreased significantly to 5.3±0.8% and 4.2±0.6% respectively, while the proportion of Escherichia coli increased to 12.5±1.3%, and the Shannon index decreased to 2.12±0.15, indicating a microbial imbalance and impaired diversity. In Examples 1-5, the proportions of Bifidobacteria and Lactobacillus were significantly higher than those in the model group, ranging from 15.6±1.8%-17.2±2.1% and 10.8±1.5%-11.8±1.8%, respectively, while the proportion of Escherichia coli was relatively low, and the Shannon index ranged from 3.52±0.18 to 3.71±0.21, showing that the examples effectively restored the balance and diversity of the microbial community. Although the comparative group outperformed the model group in some indicators, the proportions of Bifidobacterium and Lactobacillus were generally lower than those in the example group, and the Shannon index was also relatively low, indicating that the comparative group was not as effective as the example group in restoring microbial diversity.

[0115] Depend on Figure 8 , Figure 9 and Figure 10 As shown, the contents of acetic acid, propionic acid, and butyric acid in the normal group were 32.5±3.1 mmol / kg, 18.7±2.3 mmol / kg, and 15.6±1.8 mmol / kg, respectively, which were relatively high and stable. The contents of the three fatty acids in the model group were significantly reduced, with acetic acid decreasing to 12.3±1.5 mmol / kg, propionic acid to 8.5±1.2 mmol / kg, and butyric acid to 5.3±0.8 mmol / kg, indicating impaired intestinal flora metabolism. In Examples 1-5, the contents of each fatty acid increased with increasing group. In Example 5, acetic acid reached 31.2±3.3 mmol / kg, propionic acid 18.5±2.4 mmol / kg, and butyric acid 15.2±1.8 mmol / kg, close to the levels of the normal group, indicating that the intervention in the examples effectively promoted the production of SCFAs. Although the content of each fatty acid in the comparative group was higher than that in the model group, it was lower than that in the example group. For example, in comparative example 6, the content of acetic acid was 25.6±2.7 mmol / kg, propionic acid was 15.2±2.0 mmol / kg, and butyric acid was 12.1±1.4 mmol / kg, indicating that its effect on promoting the synthesis of SCFAs was not as good as that in the example, which further verified the superiority of the formulation in the example.

[0116] The gastric acid survival rate experiment is shown in Table 4 below.

[0117] Table 4 Gastric acid survival rate

[0118] Table 4 shows that the gastric acid tolerance of Examples 1-5 and Comparative Example 6 was evaluated by detecting the number of viable bacteria in the probiotics after 2 hours in an acidic gastric environment. The results showed that the initial viable bacteria count in Examples 1-5 increased from 1.2 × 10⁻⁶. 10 CFU / g increased to 2.4 × 10⁻⁶ 10 CFU / g, the viable bacterial count increased from 1.08 × 10⁻⁶ after 2 hours. 10 CFU / g increased to 2.16×10 10 The CFU / g concentration remained stable at 90%, indicating that the microencapsulation technology in the formulation effectively protects probiotics from gastric acid erosion, and that dose escalation does not affect tolerability. In contrast, Comparative Example 6 (without microencapsulation) had the same initial viable count as Example 1, but the viable count plummeted to 1.44 × 10⁻⁶ after 2 hours. 9 The CFU / g survival rate was only 12%, significantly lower than that of the Example Group. Secondly, the survival rate of Comparative Example 10 was only 50%, significantly lower than the 90% of the three-layer microcapsules, verifying the crucial role of the "sodium alginate-chitosan-maltodextrin" three-layer structure in isolating probiotics from gastric acid. Therefore, microencapsulation technology is a key factor in improving the survival rate of probiotics in gastric acid. The technical solutions in Examples 1-5 ensure that probiotics pass through the stomach in a highly active state, laying the foundation for subsequent intestinal colonization.

[0119] This application provides a composition for promoting intestinal repair, with loquat flower and leaf extract, dendrobium leaf extract, and fruit pulp matrix as the core, supplemented by probiotics and various functional excipients, to achieve intestinal repair through the synergistic effect of multiple components and mechanisms. Specifically, the triterpenoids, flavonoids, and polysaccharides obtained from the loquat flower and leaf extract through three-stage fractionation extraction can inhibit the TLR4 / NF-κB signaling pathway and thicken the intestinal mucus layer; the active ingredients obtained from the dendrobium leaf extract through compound enzymatic hydrolysis technology can activate the Wnt / β-catenin pathway, promoting intestinal villus regeneration and tight junction protein expression; the oligofructose syrup in the fruit pulp matrix, combined with loquat fruit pulp, can ferment to produce short-chain fatty acids that inhibit harmful bacteria and provide colonization sites for probiotics. Furthermore, the addition of Bifidobacterium bifidum BB-12 and Bacillus coagulans to the composition constitutes a dual antibacterial mechanism of "adhesion-rejection-membrane damage" through specific binding, creating an acidic environment, and secreting antibacterial substances. The probiotics, after being encapsulated in a three-layer microcapsule of sodium alginate, chitosan, and maltodextrin, exhibit a gastric acid survival rate of up to 90%. Sodium bicarbonate and organic acids in the excipients form a dynamic buffer to protect the probiotics and active ingredients. Curcumin nanoliposomes achieve targeted delivery and enhance anti-inflammatory effects. Glutamine, fucoidan, and β-glucan, among other components, synergistically promote intestinal repair through energy supply, barrier strengthening, and immune regulation. The preparation method employs low-temperature vacuum drying, three-gradient ethanol extraction, and complex enzymatic hydrolysis processes, effectively preserving the active ingredients and improving their dissolution rate and purity. The final composition can synergistically repair intestinal damage through multiple pathways. This solves the problems of unclear components and efficacy mechanisms, poor repair effects, slow improvement processes, and limited applicability in existing technologies.

[0120] Although the embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and are not to be construed as limitations on the present invention. A person skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. A composition for promoting intestinal repair, characterized in that, The composition comprises the following components in parts by weight: Fruit pulp substrate: 40-60 parts; Loquat flower and leaf extract: 10-15 parts; Dendrobium leaf extract: 5-10 parts; Glutamine: 5-10 parts; Fucoidan: 3-8 parts; β-glucan: 2-5 parts; Probiotics: 1-3 servings; Additional ingredients: 15-25 parts.

2. The composition for promoting intestinal repair according to claim 1, characterized in that, The fruit pulp matrix is ​​composed of fructooligosaccharide syrup and loquat pulp in a mass ratio of (1:1) to (2:1).

3. The composition for promoting intestinal repair according to claim 1, characterized in that, The preparation process of the loquat flower and leaf extract includes the following: A1. The dried and pulverized loquat flowers and leaves were extracted three times in sequence as follows; A11. Using 60-90% ethanol solution, with a solid-liquid ratio of 1:30-1:50 g / mL, reflux extraction at 60-80℃ for 1-2 hours. After concentration under reduced pressure, add 1-2 mol / L sodium hydroxide solution to adjust the pH to 9-10, let stand for 10-15 minutes, centrifuge at 4000-6000 r / min for 10-15 minutes, and precipitate as residue. The supernatant contains sodium triterpene acid salt. Add 1-2 mol / L hydrochloric acid solution to the supernatant to adjust the pH to 2-3, let stand at room temperature for 2-3 hours until the triterpene acid is completely precipitated, centrifuge to collect the precipitate, wash with purified water 2-3 times, and vacuum dry to obtain crude triterpene acid extract. A12. Add 40-60% ethanol solution to the residue of step A11, with a material-to-liquid ratio of 1:20-1:30 g / mL, and extract with ultrasonic assistance at 50-70℃ for 1-2 hours. After filtration, concentrate to obtain flavonoid extract. A13. Add pure water to the residue from step A12, with a material-to-liquid ratio of 1:10-1:20 g / mL. Extract at 80-95℃ for 2-3 hours, centrifuge at 4000 r / min for 15 minutes, take the supernatant and concentrate it under reduced pressure to 1 / 10 of the original volume, add 3-4 times the volume of 95% ethanol, let stand at 4℃ for 12 hours, centrifuge to collect the precipitate, and vacuum dry to obtain crude polysaccharide. A2. The crude triterpenic acid extract, the flavonoid extract and the crude polysaccharide are mixed in a mass ratio of (3-5):(2-4):(1-2), and small molecule impurities are removed by ultrafiltration membrane. After freeze-drying, loquat flower and leaf extract is obtained.

4. The composition for promoting intestinal repair according to claim 1, characterized in that, The preparation process of the Dendrobium leaf extract includes the following: B1. The dried and pulverized Dendrobium leaves are processed using a stepwise extraction process combined with compound enzymatic hydrolysis technology; B11. Pulverize dried Dendrobium leaves to 60-80 mesh, add cellulase and pectinase with an enzyme activity ratio of 1:1-1:2, and enzymatically hydrolyze at pH 4.5-5.5 and 45-55℃ for 30-60 minutes. After inactivating the enzyme, filter to obtain the enzymatic hydrolysate. B12. Add 70-90% ethanol solution to the enzymatic hydrolysis residue, with a material-to-liquid ratio of 1:15-1:25 g / mL, reflux at 60-70℃ for 1.5-2.5 hours, centrifuge and concentrate to obtain crude alkaloid extract. B13. Add pure water to the residue of step B12, with a material-to-liquid ratio of 1:20-1:30 g / mL, and extract at 90-100℃ for 3-4 hours. After centrifugation, take the supernatant and concentrate it. Add 3-5 times the volume of 95% ethanol for alcohol precipitation for 12-24 hours, and collect the precipitate to obtain crude polysaccharide. B2. The enzymatic hydrolysate is adsorbed through a macroporous resin column, impurities are first eluted with 20-30% ethanol, and then the target component is eluted with 50-70% ethanol. The eluent is concentrated under reduced pressure and then mixed with the crude alkaloid extract and the crude polysaccharide at a mass ratio of (1-2):(2-3):(4-5). The mixture is then spray-dried to obtain Dendrobium leaf extract.

5. The composition for promoting intestinal repair according to claim 4, characterized in that, The target component is at least one of flavonoids or phenolic acids.

6. The composition for promoting intestinal repair according to claim 1, characterized in that, The probiotics consist of Bifidobacterium bifidum BB-12 and Bacillus coagulans in a mass ratio of (1:3) to (1:4).

7. The composition for promoting intestinal repair according to claim 5, characterized in that, The viable count of the Bifidobacterium bifidum BB-12 is ≥1×10⁻⁶. 10 CFU / g, the viable count of the Bacillus coagulans is ≥5×10⁻⁶. 9 CFU / g.

8. The composition for promoting intestinal repair according to claim 1, characterized in that, The excipients comprise the following components by weight: Resistant dextrin: 10-20 parts; Sodium bicarbonate: 1-3 parts; Curcumin nanoliposomes: 0.5-2 parts; Natural loquat flavoring: 0.1-0.5 parts.

9. The composition for promoting intestinal repair according to claim 8, characterized in that, The curcumin nanoliposomes have a particle size of 50-200 nm; the resistant dextrin has a degree of polymerization of 10-20 and a water-soluble dietary fiber content of ≥90%.

10. A method for preparing a composition for promoting intestinal repair according to any one of claims 1-9, characterized in that, Includes the following steps: C1. Weigh the raw materials; C2. Pre-fermentation of fruit pulp substrate: Mix fructooligosaccharide syrup and loquat pulp, heat to 35-40℃, inoculate with Lactobacillus plantarum at an inoculation rate of 3-5%, anaerobic ferment for 12-24 hours, inactivate enzymes at 80℃ for 10 minutes, and cool for later use. C3. Prepare loquat flower and leaf extract and dendrobium leaf extract, and mix fucoidan, β-glucan and resistant dextrin and sodium bicarbonate in the excipients to obtain a premix, and pass it through an 80-100 mesh sieve for later use; C4. Heat the pre-fermented fruit pulp matrix to 35-45℃, add the loquat flower and leaf extract, the dendrobium leaf extract and glutamine in sequence, stir at 200-400 rpm for 20-30 minutes, then add the premix and homogenize it at a pressure of 10-20 MPa for 2-3 cycles. C5. Mix Bifidobacterium bifidum BB-12 with Bacillus coagulans, encapsulate the mixture in microcapsules, and then mix it with the product from step C3 under aseptic conditions with low-speed stirring. The mixture is then dried under low-temperature vacuum at a temperature of 40-50°C and a vacuum degree of -0.08 to -0.1 MPa to obtain a composition. The composition is then dispensed into powder or granules and stored under nitrogen-filled and sealed conditions.

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