Synergistic skin-brightening composition for daytime protection and night repair as well as preparation method and application of synergistic skin-brightening composition

Abstract

The invention discloses a synergistic skin-brightening composition for daytime protection and night repair as well as a preparation method and application of the synergistic skin-brightening composition, and belongs to the technical field of skin-care compositions. The composition is prepared from the following raw materials: resveratrol, glabridin, an amphiphilic nano-micelle carrier, water and a dissolution promoting auxiliary material, the dissolution promoting auxiliary material comprises at least one of paeonol, curcumin and a preservative system; the mass ratio of glabridin to resveratrol is (1-20): 1. When the glycyrrhizic acid-glabridin-resveratrol micelle is prepared, at least one of paeonol, curcumin and a preservative system is introduced, so that the content of resveratrol in the micelle can be remarkably increased, formation of transparent micelle is promoted, the particle size of the micelle is reduced, and the water solubility of the micelle is improved; therefore, the tyrosinase inhibiting and free radical scavenging capabilities of the glabridin and resveratrol composition are remarkably improved.

Description

Technical Field

[0001] This invention belongs to the field of skincare composition technology, specifically relating to a synergistic brightening composition for daytime protection and nighttime repair, its preparation method, and its application. Background Technology

[0002] With environmental degradation and increasing life pressures, symptoms such as dull skin, pigmentation, and age spots are becoming increasingly common. Improving skin tone has become an area of ​​growing importance for modern people, and the research and development of skin care products with whitening effects has also attracted much attention.

[0003] Glabridin (GLA) is an isopentenylated isoflavone extracted from the roots and rhizomes of the legume *Glycyrrhiza glabra*. Its molecular formula is C2. 20 H 20 O4. Glycyrrhizin is present in relatively small amounts in licorice root, accounting for approximately 11% of glycyrrhizin flavonoids and about 0.2% of licorice root itself, and is known as "whitening gold." Therefore, it is crucial to ensure that glycyrrhizin is effective at low concentrations or to combine it with other ingredients to achieve a synergistic effect.

[0004] Resveratrol, a natural polyphenol compound, not only exhibits excellent tyrosinase inhibition activity (with an inhibition efficiency exceeding 50%), but also effectively scavenge various free radicals such as DPPH and ABTS. These properties can complement and synergistically enhance the whitening and antioxidant effects of glycyrrhizin. Furthermore, resveratrol also possesses anti-inflammatory and anti-aging properties, which can further expand the application prospects of glycyrrhizin in anti-aging skincare products, medical dressings, and other fields.

[0005] The water solubility, transdermal absorption, and discoloration of glycyrrhizin all affect its application. ① Glycyrrhizin has a water solubility of only 1.5 mg / L, making it almost insoluble in water. This results in low bioavailability and limited biological activity, restricting its widespread use in cosmetics and topical skin preparations. ② Glycyrrhizin has a molecular weight of 324.37, which is greater than 300, slightly limiting its transdermal absorption rate in the stratum corneum (the 500 Dalton rule is the widely accepted molecular weight threshold for transdermal absorption. Studies have shown that the physical barrier function of the stratum corneum in normal skin blocks substances with a molecular weight greater than 500 Daltons. A molecular weight less than 300 is a more stringent "high-efficiency absorption threshold"). ③ Glycyrrhizin has a LOP of 4.26 and an oil-water partition coefficient greater than 4, leading to excessive retention in the stratum corneum and a decreased epidermal transfer rate. Similarly, the application of resveratrol is also limited by its low solubility, chemical instability, and poor skin permeability.

[0006] Encapsulating active ingredients of traditional Chinese medicine (TCM) in nanocarriers is a highly efficient method for delivering these ingredients to pathological sites. This method includes liposomes, polymer micelles, nanoliposome carriers, and inorganic nanoparticles. Nanoencapsulation technology offers advantages such as low toxicity, the ability to load larger amounts of active ingredients / drugs, biodegradability, and good biocompatibility. Furthermore, surface modification and linking with different ligands can achieve active targeting, and responses to different microenvironments can enable targeted release of components. Classical liposomes are bilayer closed vesicles formed by amphiphilic substances such as phospholipids, which can promote the stability, solubility, and transdermal absorption of active ingredients, thereby enhancing efficacy. In recent years, numerous studies have discovered that some TCM active ingredients, such as terpenes, possess the ability to self-assemble or co-assemble into drug delivery carriers. Compared to classical liposomes, TCM active ingredients, as nanocarriers, exhibit higher biocompatibility, lower toxicity, and fewer adverse reactions. Moreover, these ingredients themselves possess pharmacological activity and can synergistically interact with the encapsulated drugs, achieving a "drug-adjuvant" effect. Therefore, many TCM active ingredients hold promise as next-generation drug delivery carriers, offering enhanced drug delivery and disease treatment capabilities.

[0007] Studies have found that micellar excipients such as glycyrrhizic acid can encapsulate glycyrrhizin to form stable nanomicelles. However, in the preparation of micelles co-loaded with glycyrrhizin and resveratrol, the limited drug loading capacity of the glycyrrhizic acid carrier leads to a significant concentration threshold effect in the compatibility of the active ingredients. When the amount of resveratrol added is small, the two active ingredients can form a stable co-loaded system; however, when the resveratrol content increases, significant drug crystallization occurs, and hydration with pure water alone cannot form a transparent and stable nanomicelle dispersion system. This phenomenon may be related to the spatial competition effect between the two hydrophobic components in the micelle core; excessive resveratrol can disrupt the micelle structure formed by the amphiphilic glycyrrhizic acid molecules. Furthermore, this competition effect affects the synergistic effect of the two active ingredients. Therefore, further research is needed to find a method to increase the drug content in micelles co-loaded with glycyrrhizin and resveratrol to improve their efficacy. Summary of the Invention

[0008] To address the aforementioned problems in the prior art, this invention provides a synergistic skin-brightening composition for daytime protection and nighttime repair, along with its preparation method and application.

[0009] To achieve the above object, the present invention provides the following technical solutions:

[0010] This invention provides a synergistic skin-brightening composition for daytime protection and nighttime repair, comprising the following raw materials: resveratrol, glycyrrhizin, amphiphilic nanomicelle carrier, water, and solubilizing excipients; the solubilizing excipients include at least one of paeonol, curcumin, and a preservative system; the mass ratio of glycyrrhizin to resveratrol is (1-20):1.

[0011] As a preferred embodiment of the present invention, the ratio of glycyrrhizin to resveratrol is (5-20):1; when paeonol and / or curcumin are used, the mass ratio of the solubilizing excipient to glycyrrhizin is 1:(3-20); when the solubilizing excipient includes a preservative system, the preservative system accounts for 15-20 wt% of the total system.

[0012] As a preferred embodiment of the present invention, the amphiphilic nanomicelle carrier includes glycyrrhizic acid.

[0013] As a preferred embodiment of the present invention, the anti-corrosion system is composed of butanediol, 1,2-hexanediol and p-hydroxyacetophenone, wherein the content of 1,2-hexanediol is 8-12 wt%, the content of p-hydroxyacetophenone is 12-28 wt%, and the balance is butanediol.

[0014] The present invention also provides a method for preparing a synergistic skin brightening composition for daytime protection and nighttime repair as described above, comprising the following steps: weighing each raw material, first dispersing the raw materials other than water in a thin film, then adding water for hydration and dissolution to obtain nano micelles.

[0015] This invention also provides the application of the synergistic brightening composition for daytime protection and nighttime repair described above in the preparation of skin care products.

[0016] As a preferred embodiment of the present invention, the skin care product includes a skin care product with whitening effect.

[0017] Amphiphilic nanomicelle carriers, such as glycyrrhizic acid, have limited encapsulation rates. When resveratrol is introduced, it can be well encapsulated in glycyrrhizic acid-glycyrrhizin nanomicelles at low resveratrol content, exhibiting excellent water solubility. However, with increasing resveratrol content, due to the spatial competition between the two hydrophobic components within the micelle core, transparent nanomicelles cannot be obtained through water hydration alone. This invention discovers that although paeonol and curcumin are also water-insoluble active ingredients, introducing at least one of paeonol, curcumin, or a preservative system during the preparation of glycyrrhizic acid-glycyrrhizin-resveratrol micelles can significantly improve the encapsulation rate of resveratrol in glycyrrhizic acid-glycyrrhizin nanomicelles, promote the formation of transparent nanomicelles, improve the overall water solubility of the micelles, and reduce the particle size of the glycyrrhizic acid-glycyrrhizin-resveratrol nanomicelles.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] In preparing glycyrrhizic acid-glycyrrhizin-resveratrol micelles, this invention introduces at least one of paeonol, curcumin, and a preservative system, which can significantly increase the resveratrol content in the micelles, promote the formation of transparent micelles, reduce the particle size of the micelles, and improve the water solubility of the micelles, thereby significantly enhancing the ability of the glycyrrhizin and resveratrol composition to inhibit tyrosinase and scavenge free radicals.

[0020] The raw materials used in this invention are readily available and low in cost, and the preparation method is simple, making it suitable for large-scale promotion and application. Detailed Implementation

[0021] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention. It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the present invention.

[0022] Furthermore, regarding the numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Every smaller range between any stated value or intermediate value within a stated range, and any other stated value or intermediate value within said range, is also included within this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0023] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0024] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0025] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0026] The raw materials used in the following examples and comparative examples are all commercially available conventional raw materials, and are not particularly limited.

[0027] In the following examples and comparative examples, "parts" refers to parts by weight, and will not be described again below.

[0028] Example 1

[0029] According to the raw materials and dosages in Table 1, a synergistic skin-brightening composition for daytime protection and nighttime repair, namely nanomicelles, was prepared. The specific preparation method is as follows:

[0030] Preparation of common water-soluble nanomicelles: The content of glycyrrhizin was fixed at 0.1 wt% of the total system. The active ingredients were weighed according to the proportions described in Table 1. 0.5 wt% glycyrrhizic acid was weighed as a carrier. Nanomicelles were prepared by thin film dispersion. After film formation, the nanomicelles were hydrated with distilled water (after adding distilled water, the content of glycyrrhizin in the final system was 0.1 wt%), thus obtaining common water-soluble micelles.

[0031] Preparation of micelles with an anti-corrosion system: Butanediol, 1,2-hexanediol, and p-hydroxyacetophenone were prepared into a solution with a content of 70 wt%, 10 wt%, and 20 wt%, respectively, to serve as the anti-corrosion system. The preparation method was the same as that for ordinary water-soluble nanomicelles, except that 17.5 wt.% of distilled water was replaced with an equal weight of the anti-corrosion system to obtain micelles with an anti-corrosion system. The particle size of the micelles with the anti-corrosion system was tested by passing them through a 0.22 μm filter membrane.

[0032] Table 1 Raw Materials and Usage

[0033]

[0034] Effect verification

[0035] 1. The micelles prepared in Table 1 were evaluated by observing the hydration time, sample state, and particle size, as shown in Table 2.

[0036] Table 2 Evaluation of micelle preparation

[0037]

[0038]

[0039] The concentration of glycyrrhizin in the nanomicelles was fixed at 0.1 wt%. Table 2 shows that:

[0040] ① When the ratio of glycyrrhizin to resveratrol is 1:1, glycyrrhizin and resveratrol alone cannot form transparent micelles; after adding curcumin and the preservative system, transparent micelles (mice 21) can be formed, and the particle size is small; since the most difficult ratio can form nano micelles, other ratios in the preparation can also form transparent micelle solutions, so the particle size of other micelles was not tested.

[0041] ② When the ratio of glycyrrhizin to resveratrol is (2-5):1, glycyrrhizin and resveratrol alone cannot completely form transparent micelles; (2-3):1 will produce precipitation; (4-5):1 will be slightly turbid. However, after adding the preservative system, the micelle particle size is <200nm when the ratio of glycyrrhizin to resveratrol is (3-5):1. After introducing paeonol, the hydration time of micelle preparation is reduced, and the state of ordinary water-soluble micelles after hydration is improved. After adding the preservative system, particle size testing showed that the micelles with the introduction of paeonol are smaller than those without paeonol. The micelle particle size of the glycyrrhizin to resveratrol ratio of (2-5):1 is <200nm, which is significantly smaller than the particle size of the micelles without paeonol.

[0042] ③ When the ratio of glycyrrhizin to resveratrol is (10-20):1, transparent micelles can be formed. However, after the introduction of paeonol, the hydration time is reduced and the nanoparticle size becomes smaller.

[0043] In summary, the paeonol, curcumin, and preservative system significantly improve the encapsulation rate of resveratrol in glycyrrhizic acid-glycyrrhizin nanomicelles, reduce the particle size of the nanomicelles, promote the formation of transparent nanomicelles, and enhance the overall water solubility of the micelles. (It is worth noting that this preservative system is an alcohol system. Most nanomicelles will demulsify after being added to an alcohol system, resulting in the micelles not forming nano-sized particles. However, the preservative effect of adding an alcohol system to the micelles involved in this study can promote the formation of micelles.)

[0044] 2. Tyrosinase inhibition test

[0045] Test method: As shown in Table 3, L-tyrosine, sample solution, and PBS buffer were added to tubes numbered A1, A2, B1, and B2 in sequence and mixed well. Tyrosinase was added to tubes in groups A1 and B1 in sequence, and after shaking well, the mixture was spotted onto a 96-well plate. The plates were incubated at 37°C for 30 min, and the absorbance at 450 nm was measured.

[0046] The formula for calculating the inhibition rate is:

[0047]

[0048] In the formula, A1 is the absorbance of the blank sample with enzyme; A2 is the absorbance of the blank sample without enzyme; B1 is the absorbance of the sample group with enzyme; and B2 is the absorbance of the sample group without enzyme. In the experiment, the negative control was replaced with PBS buffer, and each group was measured in parallel 6 times.

[0049] Table 3 Sample dosage for tyrosinase inhibition test

[0050] reaction group V(L-tyrosine) / μL V(test sample) / μL V(PBS) / μL V (tyrosinase) / μL A1 200 0 400 100 A2 200 0 500 0 B1 200 200 200 100 B2 200 200 300 0

[0051] Table 4 shows the test results of tyrosinase inhibition rate for each group of samples.

[0052] Table 4 Results of Tyrosinase Inhibition Test

[0053]

[0054]

[0055] As shown in Table 4:

[0056] ① The total concentration of the composition remained unchanged, and the amount of glycyrrhizin was fixed at 20 parts. The proportions of each component were adjusted according to Table 1. The results showed that when the ratio of glycyrrhizin to resveratrol was (1-2):1 (i.e., 20:(10-20)), the inhibitory effect on tyrosinase activity was better after the introduction of paeonol compared with the composition without paeonol (the composition containing only resveratrol and glycyrrhizin, i.e., composition 1-2). That is, after the introduction of paeonol, the reduction of the content of resveratrol and glycyrrhizin could still achieve a comparable or better inhibitory effect on tyrosinase. Paeonol has a promoting effect on the tyrosinase inhibitory effect of the resveratrol and glycyrrhizin composition. When the ratio of glycyrrhizin to resveratrol was (4-20):1, the inhibitory effect on tyrosinase activity of the composition containing paeonol (i.e., composition 10-13) was better than that of the composition without paeonol (i.e., composition 4-7).

[0057] ② The total concentration of the composition remained unchanged, and the amount of glycyrrhizin was fixed at 10 parts. The proportions of each component were adjusted according to Table 1. The results showed that when the ratio of glycyrrhizin to resveratrol was (5-20):1, the composition containing paeonol (i.e., composition 17-18) had a better inhibitory effect on tyrosinase activity than the composition without paeonol (i.e., composition 5-6).

[0058] In summary, paeonol has the effect of increasing the inhibitory effect of the combination of glycyrrhizin and resveratrol on tyrosinase activity; when the ratio of glycyrrhizin to resveratrol is (1-5):1, the inhibitory ability of the composition containing paeonol on tyrosinase activity is comparable to or better than that of the composition without paeonol; when the ratio of glycyrrhizin to resveratrol is higher than 5:1, the inhibitory ability of the composition containing paeonol on tyrosinase activity is better than that of the composition without paeonol.

[0059] 3. Antioxidant test

[0060] Test method: Weigh 7.884 mg of 1,1-diphenyl-2-trinitrophenylhydrazine powder, dilute to 100 mL with ethanol, and shake well to obtain a 0.2 mmol DPPH solution. Weigh different samples to prepare gradient concentrations. Use a pipette to take 500 μL of sample solutions of different concentrations into plastic centrifuge tubes, add 500 μL of DPPH solution, shake well, and protect from light for 30 min. Use ethanol as a blank control.

[0061] 200 μL of absorbance was taken from each well of a 96-well plate, and the absorbance was measured at 517 nm. The experiment was performed in triplicate. The specific calculation formula is as follows:

[0062] DPPH removal rate = (1 - D1 / D2) × 100%

[0063] In the formula, D1 is the absorbance of the test sample or positive control; D2 is the absorbance of the blank control.

[0064] Table 5 shows the test results for scavenging DPPH free radicals.

[0065] Table 5 Results of DPPH free radical scavenging assay

[0066]

[0067]

[0068] From Table 5 we can see that:

[0069] ① The total concentration of the composition remained unchanged, and the amount of glycyrrhizin was fixed at 20 parts. The proportions of each component were adjusted according to Table 1. The results showed that when the ratio of glycyrrhizin to resveratrol was (1-2):1, the DPPH free radical scavenging ability after adding paeonol was not as good as that of the composition without paeonol (i.e., the composition containing only resveratrol and glycyrrhizin, i.e., composition 1-2); however, when the ratio of glycyrrhizin to resveratrol was 4:1, the composition containing paeonol (i.e., composition 10) had a better DPPH free radical scavenging ability at a low concentration (62.5 ug / mL) than the composition without paeonol (composition 4); when the ratio of glycyrrhizin to resveratrol was (5-20):1, the composition containing paeonol (i.e., composition 11-13) had a better DPPH free radical scavenging ability at both concentrations than the composition without paeonol (i.e., composition 5-7).

[0070] ② The total concentration of the composition remained unchanged, and the amount of glycyrrhizin was fixed at 10 parts. The proportions of each component were adjusted according to Table 1. The results showed that when the ratio of glycyrrhizin to resveratrol was 1:1, the DPPH free radical scavenging ability after adding paeonol was not as good as that of composition 1 without paeonol. However, when the ratio of glycyrrhizin to resveratrol was 2:1, the composition containing paeonol (i.e., composition 15) had a better DPPH free radical scavenging ability at a low concentration (6.25 ug / mL) than the composition without paeonol (i.e., composition 2). Comparing the ratio of glycyrrhizin:resveratrol:paeonol in composition 9 (20:10:1) and glycyrrhizin:resveratrol:paeonol in composition 15 (10:5:1, i.e., 20:10:2), it can be concluded that increasing the amount of paeonol can promote the antioxidant effect of the glycyrrhizin and resveratrol composition. When the ratio of glycyrrhizin to resveratrol was 3:1, the composition containing paeonol (i.e., composition 20) showed better DPPH radical scavenging ability at a low concentration (62.5 μg / mL) than the composition without paeonol (composition 3). When the ratio of glycyrrhizin to resveratrol was (5–10):1, the compositions containing paeonol (i.e., compositions 17–18) showed better DPPH radical scavenging ability at both concentrations than the compositions without paeonol (i.e., compositions 5–6).

[0071] ③ Keeping the total concentration of the composition constant, and fixing the amount of glycyrrhizin at 5 parts, the ratio of resveratrol and paeonol was adjusted according to Table 1. The results showed that when the ratio of glycyrrhizin to resveratrol was 5:1, the composition containing paeonol (i.e., composition 19) had better DPPH free radical scavenging ability than the composition without paeonol (i.e., composition 5) at both concentrations.

[0072] ④ The total concentration of the composition remained unchanged, and the amount of glycyrrhizin was fixed at 3 parts. The ratio of resveratrol and paeonol was adjusted according to Table 1. The results showed that when the ratio of glycyrrhizin to resveratrol was 3:1, the composition containing paeonol (i.e., composition 20) had a better DPPH free radical scavenging ability at low concentration (62.5 ug / mL) than the composition without paeonol (composition 3).

[0073] In summary, paeonol can enhance the antioxidant effect of the combination of glycyrrhizin and resveratrol. When the ratio of glycyrrhizin to resveratrol is (2-3):1, the composition containing paeonol has a better DPPH free radical scavenging ability at low concentrations than the composition without paeonol. When the ratio of glycyrrhizin to resveratrol is 5-20:1, the composition containing paeonol has a better DPPH free radical scavenging ability at both concentrations than the composition without paeonol.

[0074] The above test results indicate that the addition of paeonol or curcumin significantly enhances the efficacy (antioxidant and tyrosinase inhibition) and micelle formation (stability, encapsulation rate): First, paeonol or curcumin promotes the antioxidant and tyrosinase-inhibiting effects of the glycyrrhizin-resveratrol composition. When the ratio of glycyrrhizin to resveratrol is (5-20):1, the composition containing paeonol shows superior inhibitory ability against tyrosinase activity and DPPH free radical scavenging ability compared to the composition without paeonol. Second, in the preparation of glycyrrhizic acid-glycyrrhizin-resveratrol micelles, the addition of at least one of paeonol, curcumin, or an alcoholic solution can significantly increase the resveratrol content in the micelles, promote the formation of transparent micelles, reduce the micelle particle size, and improve the water solubility of the micelles.

[0075] When the mass ratio of glycyrrhizin to resveratrol was (1–5):1, the addition of paeonol significantly improved the precipitation problem (i.e., encapsulating resveratrol), but some micelles also showed instability, such as micelle 14. Secondly, regarding the antioxidant and whitening effects, when the mass ratio of glycyrrhizin to resveratrol was (1–5):1, the activity of some micelles was lower than that of the comparative sample, while when the mass ratio of glycyrrhizin to resveratrol was (5–20):1, the activity was higher than that of the comparative sample.

[0076] Therefore, a mass ratio of 5:1 of glycyrrhizin to resveratrol can be regarded as the "critical point" for micelle stability and efficacy. Based on a comprehensive consideration of product stability and efficacy, a mass ratio of (5-20):1 of glycyrrhizin to resveratrol is selected.

[0077] The tyrosinase inhibition test and antioxidant test mentioned above were conducted on nanomicelles without a preservative system. The results of the tyrosinase inhibition test and antioxidant test on nanomicelles with a preservative system were similar to those of the above test results, and the patterns were consistent.

[0078] In the above tests, the total concentration of glycyrrhizin in the nanomicelles was 0.1 wt%, but the preparation method of the present invention can encapsulate a larger amount of glycyrrhizin (concentrations of 1 wt%, 2 wt%, etc.).

[0079] The above description is merely a preferred embodiment of the present invention, and the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A synergistic brightening composition for daytime protection and nighttime repair, characterized in that, The raw materials include: resveratrol, glycyrrhizin, amphiphilic nanomicelle carrier, water and solubilizing excipients; the solubilizing excipients include at least one of paeonol, curcumin and a preservative system; the mass ratio of glycyrrhizin to resveratrol is (1-20):

1.

2. The synergistic brightening composition for daytime protection and nighttime repair according to claim 1, characterized in that, The ratio of glycyrrhizin to resveratrol is (5-20):1; when the solubilizing excipient is paeonol and / or curcumin, the mass ratio of the solubilizing excipient to glycyrrhizin is 1:(3-20); when the solubilizing excipient includes a preservative system, the preservative system accounts for 15-20 wt% of the total system.

3. The synergistic brightening composition for daytime protection and nighttime repair according to claim 1, characterized in that, The amphiphilic nanomicelle carrier includes glycyrrhizic acid.

4. The synergistic brightening composition for daytime protection and nighttime repair according to claim 1, characterized in that, The corrosion protection system is composed of butanediol, 1,2-hexanediol and p-hydroxyacetophenone, wherein the content of 1,2-hexanediol is 8-12 wt%, the content of p-hydroxyacetophenone is 12-28 wt%, and the balance is butanediol.

5. A method for preparing a synergistic skin-brightening composition for daytime protection and nighttime repair according to any one of claims 1 to 4, characterized in that, The process includes the following steps: weighing each raw material, first dispersing the raw materials other than water in a thin film, then adding water to hydrate and dissolve them to obtain nano micelles.

6. The use of a synergistic brightening composition for daytime protection and nighttime repair according to any one of claims 1 to 4 in the preparation of skin care products.

7. The application according to claim 6, characterized in that, The skincare products mentioned include those with whitening effects.

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