Selenium-rich Chinese herbal medicine pill for consolidating basis and preparation method of selenium-rich Chinese herbal medicine pill

Abstract

The invention discloses a preparation method of selenium-enriched Chinese herbal medicine pills for consolidating basis, which belongs to the technical field of nutrient enrichment and is realized by five steps of biological enhancement planting, compound enzyme hydrolysis, two-stage fermentation, calcination reaction and finished product preparation. According to the preparation method of the selenium-rich traditional Chinese herbal medicine pill for consolidating basis and cultivating primordial qi, the selenium content in prepared polygonum multiflorum, astragalus membranaceus and nutgrass flatsedge is increased in a selenium biological enhancement planting mode, and then protein and polysaccharide in enzymolysis products are degraded into small molecule peptides and oligosaccharides through compound enzyme hydrolysis and two-time fermentation, so that the selenium-rich traditional Chinese herbal medicine pill for consolidating basis and cultivating primordial qi can be easily absorbed by a human body. And finally, by calcining the sea clam shells and mixing the sea clam shells with the fermentation liquor, the utilization rate of selenium can be further increased. The whole process is simple and easy to implement, convenient to operate, low in production cost and suitable for industrial large-scale production.

Description

Technical Field

[0001] This invention relates to the field of nutritional fortification technology, and more specifically, to a method for preparing a selenium-enriched herbal pill for strengthening the body and nourishing vital energy. Background Technology

[0002] Selenium is one of the essential trace elements for the human body, playing a vital role in maintaining normal physiological functions. Studies have shown that selenium deficiency can lead to various diseases, such as endemic diseases like Keshan disease and Kashin-Beck disease, as well as common diseases like cardiovascular disease and cancer. With economic development and improved living standards, people are paying more and more attention to health, and the demand for selenium supplementation is increasing. Currently, selenium products sold on the market mainly fall into two categories: one is supplements with chemically synthesized products such as sodium selenite and selenized carrageenan as the main ingredients; the other is supplementation achieved by consuming selenium-rich foods or taking selenium-containing health products.

[0003] In recent years, scholars both at home and abroad have conducted extensive research on how to increase the selenium content in plants and have achieved certain results. Among them, biofortification technology is considered an effective and safe method.

[0004] Biofortification refers to the technology of increasing the content of certain nutrients in food using biological methods. This technology mainly includes two types: genetic engineering and non-GMO methods. Genetic engineering technology aims to increase the production of specific nutrients by introducing foreign genes into plant cells. Non-GMO methods, on the other hand, promote the accumulation of certain nutrients by regulating the plant's growth environment without altering its original genetic information. Commonly used non-GMO methods include: soil improvement; foliar spraying; root soaking; and seed treatment.

[0005] Among these methods, soil improvement is the most common. However, because different crops have significantly different selenium requirements (vegetables generally require higher amounts), it's difficult to solve all problems once and for all. Furthermore, the high cost, long timeframe, and slow results of soil improvement are also significant drawbacks. In contrast, other methods can quickly and effectively increase the selenium content in agricultural products. Summary of the Invention

[0006] 1. Technical problems to be solved

[0007] In view of the problems existing in the prior art, the purpose of this invention is to provide a method for preparing selenium-enriched herbal pills for strengthening the body and nourishing vital energy. This method can further improve the utilization rate of selenium by calcining clam shells and mixing them with fermentation liquid.

[0008] 2. Technical Solution

[0009] To solve the above problems, the present invention adopts the following technical solution.

[0010] This invention discloses a method for preparing a selenium-enriched, health-strengthening traditional Chinese medicine pill, comprising the following steps:

[0011] 1) Processed Polygonum multiflorum, Astragalus membranaceus, and Cyperus rotundus were subjected to selenium biofortification cultivation, among which:

[0012] Processed Polygonum multiflorum should be grown in soil with a selenium content of 20-50 mg / kg;

[0013] Spray Astragalus membranaceus and Cyperus rotundus with a 0.05-0.2 wt% selenomethionine solution 3-5 times during their growing season;

[0014] 2) Mix the raw materials obtained in step 1), add a compound enzyme for hydrolysis. The compound enzyme is composed of cellulase and protease in a mass ratio of 2:1. The amount of enzyme added is 0.4-0.6% cellulase and 0.2-0.4% protease based on the dry weight of the raw materials. Hydrolyze at 40-50°C for 2-4 hours.

[0015] 3) The enzyme hydrolysate is fermented in two stages:

[0016] First stage: Inoculate with 5-10 vol% Lactobacillus plantarum and ferment at 35-38℃ for 24-48 hours;

[0017] Second stage: Inoculate with 3-8 vol% of thermophilic streptococci and ferment at 40-42°C for 12-24 hours;

[0018] 4) Calcine the clam shells at 800–1000℃ for 2 hours, crush them, and mix them with the fermentation liquid from step 3). Add CaCl2 solution to adjust the Ca content. 2+ When the concentration reaches 0.08–0.12 mol / L, the reaction produces calcium selenate inclusion complexes;

[0019] 5) Concentrate the mixture obtained in step 4) to a solid content of 40-60%, add 20-30% microcrystalline cellulose by dry weight of the material, and pelletize by extrusion and spheronization.

[0020] Furthermore, in step 1), the planting time for processed Polygonum multiflorum is 1 to 2 years, and the planting time for Astragalus membranaceus and Cyperus rotundus is about half a year. No further fertilization is required during the planting period.

[0021] Further, in step 2), the composite enzyme is a mixed enzyme preparation composed of cellulase and protease in a mass ratio of 2:1, wherein the amount of cellulase added is 0.4-0.6% of the dry weight of the raw materials, and the amount of protease added is 0.2-0.4% of the dry weight of the raw materials.

[0022] Further, in step 3), the *Lactobacillus plantarum* is *Lactobacillus thermophilus*, and the *Streptococcus thermophilus* is *Streptococcus thermophilus*.

[0023] Further, in step 4), the mass concentration of the CaCl2 solution is 0.08–0.12 mol / L, preferably 0.09 mol / L.

[0024] Furthermore, in step 5), the concentration temperature is 80-90°C, the stirring speed is 100-200 r / min, and the concentration time does not exceed 1 hour.

[0025] This invention also discloses a selenium-enriched herbal pill for strengthening the body and nourishing vital energy, prepared by the above method, comprising: selenium bio-enhanced processed Polygonum multiflorum, Astragalus membranaceus, and Cyperus rotundus enzymatic hydrolysate fermentation product; calcined clam shell and calcium selenate inclusion complex; microcrystalline cellulose; wherein the selenium element exists in both organic selenium (enzymatic hydrolysate fermentation product) and inorganic selenium (calcium selenate inclusion complex), and the total selenium content is 30-35 μg / g.

[0026] The present invention also discloses a health food comprising the above-mentioned selenium-enriched herbal pills for strengthening the body and nourishing vital energy, and pharmaceutically acceptable excipients.

[0027] This invention also discloses the application of the above-mentioned selenium-enriched herbal pills for strengthening the body and nourishing vital energy in the preparation of health foods with antioxidant functions.

[0028] 3. Beneficial effects

[0029] Compared to existing technologies, the advantages of this invention are as follows: The method for preparing selenium-enriched herbal pills provided by this invention increases the selenium content in processed Polygonum multiflorum, Astragalus membranaceus, and Cyperus rotundus through selenium bio-enhanced cultivation. Then, through compound enzymatic hydrolysis and two fermentations, the proteins and polysaccharides in the enzymatic hydrolysate are degraded into small molecule peptides and oligosaccharides, thus facilitating human absorption. Finally, calcining clam shells and mixing them with the fermentation broth further improves the utilization rate of selenium. The entire process is simple, easy to operate, and has low production costs, making it suitable for large-scale industrial production. Detailed Implementation

[0030] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0031] Example 1:

[0032] This embodiment provides a method for preparing selenium-enriched herbal pills for strengthening the body and nourishing vital energy, including the following steps:

[0033] 1) Processed Polygonum multiflorum, Astragalus membranaceus, and Cyperus rotundus were subjected to selenium biofortification cultivation, among which:

[0034] Processed Polygonum multiflorum was grown for one year in soil with a selenium content of 20 mg / kg;

[0035] Spray Astragalus membranaceus and Cyperus rotundus with a 0.05wt% selenomethionine solution three times during their growing season;

[0036] 2) Mix the raw materials obtained in step 1), add a compound enzyme for hydrolysis. The compound enzyme is composed of cellulase and protease in a mass ratio of 2:1. The amount of enzyme added is 0.4% cellulase and 0.2% protease of the dry weight of the raw materials. Hydrolyze at 40°C for 2 hours.

[0037] 3) The enzyme hydrolysate is fermented in two stages:

[0038] First stage: Inoculate with 5 vol% Lactobacillus plantarum and ferment at 35°C for 24 hours;

[0039] Second stage: Inoculate with 3 vol% of thermophilic streptococci and ferment at 40°C for 12 hours;

[0040] 4) Calcine the clam shells at 800℃ for 2 hours, crush them, and mix them with the fermentation liquid from step 3). Add CaCl2 solution to adjust the Ca content. 2+ When the concentration is increased to 0.08 mol / L, the reaction produces calcium selenate inclusion complex;

[0041] 5) Concentrate the mixture obtained in step 4) to a solid content of 40%, add 20% of the dry weight of microcrystalline cellulose, and pelletize by extrusion and spheronization.

[0042] The obtained pills were tested, and the results showed that the selenium content of the pills was 30 μg / g.

[0043] Example 2

[0044] This embodiment provides a method for preparing selenium-enriched herbal pills for strengthening the body and nourishing vital energy, including the following steps:

[0045] 1) Processed Polygonum multiflorum, Astragalus membranaceus, and Cyperus rotundus were subjected to selenium biofortification cultivation, among which:

[0046] Processed Polygonum multiflorum was grown in soil with a selenium content of 30 mg / kg for one and a half years;

[0047] Astragalus and Cyperus were sprayed with a 0.1 wt% selenomethionine solution four times during their growing season;

[0048] 2) Mix the raw materials obtained in step 1), add a compound enzyme for hydrolysis. The compound enzyme is composed of cellulase and protease in a mass ratio of 2:1. The amount of enzyme added is 0.5% cellulase and 0.3% protease of the dry weight of the raw materials. Hydrolyze at 45°C for 3 hours.

[0049] 3) The enzyme hydrolysate is fermented in two stages:

[0050] First stage: Inoculate with 8 vol% Lactobacillus plantarum and ferment at 38°C for 36 hours;

[0051] Second stage: Inoculate with 5 vol% Streptococcus thermophilus and ferment at 42°C for 18 hours;

[0052] 4) Calcine the clam shells at 900℃ for 2 hours, crush them, mix them with the fermentation liquid from step 3), and add CaCl2 solution to adjust the Ca content. 2+ When the concentration is increased to 0.09 mol / L, the reaction produces calcium selenate inclusion complex;

[0053] 5) Concentrate the mixture obtained in step 4) to a solid content of 50%, add 25% microcrystalline cellulose by dry weight of the material, and pelletize by extrusion and spheronization.

[0054] The obtained pills were tested, and the results showed that the selenium content of the pills was 33 μg / g.

[0055] Example 3

[0056] This embodiment provides a method for preparing selenium-enriched herbal pills for strengthening the body and nourishing vital energy, including the following steps:

[0057] 1) Processed Polygonum multiflorum, Astragalus membranaceus, and Cyperus rotundus were subjected to selenium biofortification cultivation, among which:

[0058] Processed Polygonum multiflorum was grown for 2 years in soil with a selenium content of 40 mg / kg;

[0059] Astragalus and Cyperus were sprayed with a 0.2wt% selenomethionine solution five times during their growing season.

[0060] 2) Mix the raw materials obtained in step 1), add a compound enzyme for hydrolysis. The compound enzyme is composed of cellulase and protease in a mass ratio of 2:1. The amount of enzyme added is 0.6% cellulase and 0.4% protease of the dry weight of the raw materials. Hydrolyze at 50°C for 4 hours.

[0061] 3) The enzyme hydrolysate is fermented in two stages:

[0062] First stage: Inoculate with 10 vol% Lactobacillus plantarum and ferment at 38°C for 48 hours;

[0063] Second stage: Inoculate with 8 vol% Streptococcus thermophilus and ferment at 42°C for 24 hours;

[0064] 4) Calcine the clam shells at 1000℃ for 2 hours, crush them, mix them with the fermentation liquid from step 3), and add CaCl2 solution to adjust the Ca content. 2+ When the concentration is increased to 0.12 mol / L, the reaction produces calcium selenate inclusion complex;

[0065] 5) Concentrate the mixture obtained in step 4) to a solid content of 60%, add 30% microcrystalline cellulose by dry weight of the material, and pelletize by extrusion and spheronization.

[0066] The obtained pills were tested, and the results showed that the selenium content of the pills was 35 μg / g.

[0067] Comparative Example 1

[0068] This comparative example provides a method for preparing selenium-enriched herbal pills for strengthening the body and nourishing vital energy, including the following steps:

[0069] 1) Processed Polygonum multiflorum, Astragalus membranaceus, and Cyperus rotundus were subjected to selenium biofortification cultivation, among which:

[0070] Processed Polygonum multiflorum was grown for one year in soil with a selenium content of 20 mg / kg;

[0071] Spray Astragalus membranaceus and Cyperus rotundus with a 0.05wt% selenomethionine solution three times during their growing season;

[0072] 2) Mix the raw materials obtained in step 1), add a compound enzyme for hydrolysis. The compound enzyme is composed of cellulase and protease in a mass ratio of 2:1. The amount of enzyme added is 0.4% cellulase and 0.2% protease of the dry weight of the raw materials. Hydrolyze at 40°C for 2 hours.

[0073] 3) The enzyme hydrolysate is fermented in two stages:

[0074] First stage: Inoculate with 5 vol% Lactobacillus plantarum and ferment at 35°C for 24 hours;

[0075] Second stage: Inoculate with 3 vol% Streptococcus thermophilus and ferment at 40°C for 12 hours;

[0076] 4) Calcine the clam shells at 800℃ for 2 hours, crush them, and mix them with the fermentation liquid from step 3). Add CaCl2 solution to adjust the Ca content. 2+ When the concentration is increased to 0.08 mol / L, the reaction produces calcium selenate inclusion complex;

[0077] 5) Concentrate the mixture obtained in step 4) to a solid content of 40%, add 20% of the dry weight of microcrystalline cellulose, and pelletize by extrusion and spheronization.

[0078] The obtained pills were tested, and the results showed that the selenium content of the pills was 28 μg / g.

[0079] Comparative Example 2

[0080] This comparative example provides a method for preparing selenium-enriched herbal pills for strengthening the body and nourishing vital energy, including the following steps:

[0081] 1) Processed Polygonum multiflorum, Astragalus membranaceus, and Cyperus rotundus were subjected to selenium biofortification cultivation, among which:

[0082] Processed Polygonum multiflorum was grown for one year in soil with a selenium content of 20 mg / kg;

[0083] Spray Astragalus membranaceus and Cyperus rotundus with a 0.05wt% selenomethionine solution three times during their growing season;

[0084] 2) Mix the raw materials obtained in step 1), add a compound enzyme for hydrolysis. The compound enzyme is composed of cellulase and protease in a mass ratio of 2:1. The amount of enzyme added is 0.4% cellulase and 0.2% protease of the dry weight of the raw materials. Hydrolyze at 40°C for 2 hours.

[0085] 3) The enzyme hydrolysate is fermented in two stages:

[0086] First stage: Inoculate with 5 vol% Lactobacillus plantarum and ferment at 35°C for 24 hours;

[0087] Second stage: Inoculate with 3 vol% Streptococcus thermophilus and ferment at 40°C for 12 hours;

[0088] 4) Calcine the clam shells at 800℃ for 2 hours, crush them, and mix them with the fermentation liquid from step 3). Add CaCl2 solution to adjust the Ca content. 2+ When the concentration is increased to 0.08 mol / L, the reaction produces calcium selenate inclusion complex;

[0089] 5) Concentrate the mixture obtained in step 4) to a solid content of 40%, add 20% of the dry weight of microcrystalline cellulose, and pelletize by extrusion and spheronization.

[0090] The obtained pills were tested, and the results showed that the selenium content of the pills was 32 μg / g.

[0091] Experimental testing shows that the preparation method of the selenium-enriched herbal pills provided by this invention increases the selenium content in processed Polygonum multiflorum, Astragalus membranaceus, and Cyperus rotundus through selenium-enhanced bio-cultivation. Then, through compound enzymatic hydrolysis and two fermentations, the proteins and polysaccharides in the enzymatic hydrolysates are degraded into small molecule peptides and oligosaccharides, thus facilitating human absorption. Finally, calcining clam shells and mixing them with the fermentation broth further improves the utilization rate of selenium. The entire process is simple, easy to operate, and has low production costs, making it suitable for large-scale industrial production.

[0092] The above description is merely a preferred embodiment of the present invention; however, the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and its improved concepts, should be covered within the scope of protection of the present invention.

Claims

1. A method for preparing a selenium-enriched, traditional Chinese medicine pill for strengthening the body and nourishing vital energy, characterized in that... Includes the following steps: 1) Processed Polygonum multiflorum, Astragalus membranaceus, and Cyperus rotundus were subjected to selenium biofortification cultivation, among which: Processed Polygonum multiflorum should be grown in soil with a selenium content of 20-50 mg / kg; Spray Astragalus membranaceus and Cyperus rotundus with a 0.05-0.2 wt% selenomethionine solution 3-5 times during their growing season; 2) Mix the raw materials obtained in step 1), add a compound enzyme for hydrolysis. The compound enzyme is composed of cellulase and protease in a mass ratio of 2:

1. The amount of enzyme added is 0.4-0.6% cellulase and 0.2-0.4% protease based on the dry weight of the raw materials. Hydrolyze at 40-50°C for 2-4 hours. 3) The enzyme hydrolysate is fermented in two stages: First stage: Inoculate with 5-10 vol% Lactobacillus plantarum and ferment at 35-38℃ for 24-48 hours; Second stage: Inoculate with 3-8 vol% of thermophilic streptococci and ferment at 40-42°C for 12-24 hours; 4) Calcine the clam shells at 800–1000℃ for 2 hours, crush them, and mix them with the fermentation liquid from step 3). Add CaCl2 solution to adjust the Ca content. 2+ When the concentration reaches 0.08–0.12 mol / L, the reaction produces calcium selenate inclusion complexes; 5) Concentrate the mixture obtained in step 4) to a solid content of 40-60%, add 20-30% microcrystalline cellulose by dry weight of the material, and pelletize by extrusion and spheronization.

2. The method for preparing a selenium-enriched herbal pill for strengthening the body and nourishing vital energy according to claim 1, characterized in that: In step 4), the calcium selenate inclusion complex formation rate is 45-55%.

3. The method for preparing a selenium-enriched herbal pill for strengthening the body and nourishing vital energy according to claim 1, characterized in that: The selenium content of the resulting pills is 30–35 μg / g.

4. A selenium-enriched herbal pill for strengthening the body and nourishing vital energy, characterized in that: Prepared by the method according to any one of claims 1 to 3, comprising: selenium bio-enhanced processed Polygonum multiflorum, Astragalus membranaceus, and Cyperus rotundus enzymatic hydrolysate fermentation product; calcined clam shell and calcium selenate inclusion complex; microcrystalline cellulose; wherein selenium exists in both organic selenium (enzymatic hydrolysate fermentation product) and inorganic selenium (calcium selenate inclusion complex), and the total selenium content is 30-35 μg / g.

5. The method for preparing a selenium-enriched herbal pill for strengthening the body and nourishing vital energy according to claim 1, characterized in that: The total amount of selenium in the pills obtained in step 4) is 30 μg / g to 35 μg / g.

6. A health food product, characterized in that... It includes the selenium-enriched herbal pills for strengthening the body and nourishing vital energy as described in claim 4, and pharmaceutically acceptable excipients.

7. The application of a selenium-enriched herbal pill for strengthening the body and nourishing vital energy as described in claim 4 in the preparation of health food with antioxidant function.