Pure traditional Chinese medicine for oral administration and external application of cattle and sheep intestinal mastitis

Abstract

The invention relates to the field of treatment of mastitis in cattle and sheep breeding in biological agriculture, in particular to a pure traditional Chinese medicine oral administration and external application dual-way medicine for cattle and sheep intestinal mastitis. The oral prescription comprises the following main medicines in parts by weight: 15-18 parts of dandelion, 17 parts of salvia miltiorrhiza, 12 parts of pomegranate peel extract and 15 parts of attapulgite; the oral composition adjuvant comprises 6-8 parts of asparagus, 5-7 parts of ligusticum wallichii, 5 parts of an echinacea extract or 5 parts of astragalus polysaccharide; the ratio of lactobacillus plantarum to lactobacillus acidophilus compound bacterium powder is 2: 1 (15 parts); and the external application formula comprises 48 parts of main medicines: 15 parts of selfheal, 15 parts of philippine violet herb, 8 parts of ricepaperplant pith, 8 parts of frankincense and 2 parts of baicalin. According to the device, the medicine external application function and the milking function are combined, the treatment efficiency is improved, the stress response to animals is reduced, and the whole treatment process is more humanized and efficient.

Description

Technical Field

[0001] This invention relates to the treatment of mastitis in cattle and sheep raised in bio-agriculture, and particularly to a pure traditional Chinese medicine drug for enterogenic mastitis in cattle and sheep that can be administered orally and applied topically. Background Technology

[0002] During animal treatment, animals may exhibit uncooperative behavior, which typically involves the following aspects: Fear of treatment tools or medications: Animals may feel unfamiliar and fearful of the tools used during treatment, such as sprayers, syringes, etc. Additionally, the smell, taste, or texture of the medication may also cause resistance and anxiety in animals. During treatment, if the animal experiences pain when the injured area is touched or medication is injected, it may struggle to escape the pain. Animals are easily agitated in unfamiliar or noisy environments, thus refusing treatment. Adverse reactions after treatment (such as bone marrow suppression, gastrointestinal discomfort, etc.) may lead to a poor mental state in animals, thereby affecting their cooperation.

[0003] Applying medication directly to the udder of cows or goats is difficult and can easily lead to injury. Generally, both cows and goats can adapt to the use of milking machines, but this process can take time and requires patience and consistency. In practice, farmers take several measures to help cows and goats acclimatize to the milking machine. For example, during initial use, they may first hand-milk to stimulate the teats before attaching the milking machine. This helps the animals relax and reduces stress from the new equipment. Furthermore, the milking machine is designed with animal welfare in mind; for instance, parts made of soft materials gently contact the udder, increasing comfort.

[0004] Over time, most dairy cows and goats gradually get used to the presence of milking machines and are able to accept the daily milking process relatively calmly.

[0005] On February 25, 2025, a search was conducted in the China Patent Publication Database using "(cow or sheep) and mammary gland and medicine and external application and (cone or sheath)" as the abstract keywords, with the option to allow synonym expansion. No relevant literature was found.

[0006] On February 25, 2025, an abstract search was conducted on CNKI (China National Knowledge Infrastructure) using the keywords "(cow or sheep) and mammary gland and medicine and external application and (cone or sheath)". No relevant literature was found.

[0007] On February 25, 2025, a search was conducted on the website of the United States Patent and Trademark Office for the term “(Cattle or Sheep) WITH Mammary Gl WITHs WITH Drug WITH External Application WITH (Cone or Sleeve)”, but no relevant literature was found; the search URL is https: / / ppubs.uspto.gov / pubwebapp / .

[0008] On February 25, 2025, a search was conducted on WIPO's website https: / / patentscope2.wipo.int / with the search term "(Cattle or Sheep)AND Mammary Glands AND Drug AND External Application AND (Cone or Sleeve)", but no relevant literature was found.

[0009] On February 25, 2025, a search was conducted on the website of the Japan Patent Office (https: / / www.j-platpat.inpit.go.jp / ) for the search term "Sheep AND Mammary Glands AND Drug AND External Application AND Cone", but no relevant literature was found.

[0010] The search was then expanded, and the following drug literature on bovine mammary glands was found (none of the following drugs had combination for external or internal use or specific animal administration structures):

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Summary of the Invention

[0017] Purpose of the invention: To provide a more effective pure traditional Chinese medicine drug for enterogenic mastitis in cattle and sheep, administered both orally and topically. Specific objectives are detailed in the specific implementation section, which outlines several substantive technical effects.

[0018] To achieve the above objectives, the present invention adopts the following technical solution:

[0019] A pure traditional Chinese medicine treatment for enterogenic mastitis in cattle and sheep, administered orally and applied topically, characterized by:

[0020] The following oral and topical formulations are included in the following weight proportions:

[0021] The oral formulation is a feed additive for daily prevention.

[0022] The main ingredients of the oral medication formula are: dandelion (15-18 parts), salvia miltiorrhiza (17 parts), pomegranate peel extract (12 parts), and attapulgite (15 parts).

[0023] Oral adjuvants: Asparagus cochinchinensis (6-8 parts), Ligusticum chuanxiong (5-7 parts), Echinacea purpurea extract (5 parts) or Astragalus polysaccharide (5 parts);

[0024] Oral formulation of probiotic preparation: Lactobacillus plantarum: Lactobacillus acidophilus compound powder = 2:1 (15 parts);

[0025] The topical formulation is a local transdermal type, used for treatment during the onset of the disease;

[0026] The main ingredients of the external application formula are 48 parts: Prunella vulgaris (15 parts), Viola yedoensis (15 parts), Tetrapanax papyriferus (8 parts), Boswellia carterii (8 parts), and baicalin (2 parts).

[0027] External application formulation with 10 parts penetration enhancer: azone (4 parts), borneol (5.5 parts), menthol (0.5 parts)

[0028] 42 parts of the external application formulation carrier: ① Thermosensitive gel matrix (28 parts) (Poloxamer 407 + Poloxamer 188 = 3:1) ② Carbomer (1 part), curing temperature 33-37 degrees Celsius, curing into a film after contact with the breast (≤2 min), prolonging drug release to 8-12 hours. ③ Attapulgite (13 parts).

[0029] A further technical solution of the present invention is that the oral formulation also contains the following substances:

[0030] Oral formulation of taste masking agent: 0.35 parts in total, including 0.2 parts steviol glycoside + 0.1 parts menthol + 0.05 parts sweetness-enhancing peptide, used to circumvent the bitterness threshold defect of single steviol glycoside.

[0031] A further technical solution of the present invention is that the carrier filler of the oral formulation is 8 parts of wheat bran.

[0032] A further technical solution of the present invention is that the external application formula includes a kneading and dispensing system based on an improved breast pump;

[0033] The improved kneading and dispensing system based on the breast pump has an external thread 2 on the outer shell of the milk cup 1;

[0034] It also includes a rotating cup 3, which has internal threads inside;

[0035] The rotating cup 3 can be threaded onto the external thread 2, and rotating the rotating cup 3 can cause the rotating cup 3 to rise and fall.

[0036] The rotating cup 3 is connected to a drug delivery structure 5, which is a conical structure. The inner wall 6 of the drug delivery structure 5 can adhere to the external medication.

[0037] A further technical solution of the present invention is that the inner wall 6 can adhere to release paper with external medication.

[0038] A further technical solution of the present invention is that the dosing structure 5 is made of rubber.

[0039] A further technical solution of the present invention is that the dosing structure is an inflatable latex material.

[0040] A further technical solution of the present invention is that the inflatable latex material can conform to the udder area of ​​cattle and sheep after inflation.

[0041] A further technical solution of the present invention is that it also includes an auxiliary infrared temperature sensor, which is capable of measuring the temperature of the udder of cattle and sheep.

[0042] The present invention, employing the above technical solution, offers the following advantages over existing technologies: By combining a device for applying external medication with a milking machine, the problem of animal intolerance and non-cooperation can be solved, while simultaneously reducing the risks and workload of manual labor. In this way, medication can be applied directly to the udder while the animal is being milked, thus avoiding the difficulties and risks associated with applying medication separately. This device, combining external medication application and milking functions, not only improves treatment efficiency but also reduces stress on the animal, making the entire treatment process more humane and efficient. Attached Figure Description

[0043] To further illustrate the present invention, the following description is provided in conjunction with the accompanying drawings:

[0044] Figure 1 This is an improved version of the milk cup;

[0045] Figure 2 Another structural diagram of the improved milk cup;

[0046] The components include: 1. Milk cup; 2. External thread; 3. Rotating cup; 4. Nipple clamping port; 5. Medication dispensing structure; 6. Inner wall; 7. Air inlet. Detailed Implementation

[0047] The present invention will be further illustrated below with reference to the accompanying drawings and specific embodiments. It should be understood that the following specific embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. In the description of the present invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," "outer," "top," and "bottom," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the present invention. In addition, unless otherwise expressly specified and limited, the terms "installed," "connected," and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal communication of two components. For those skilled in the art, the specific meaning of the above terms in the present invention can be understood according to the specific circumstances.

[0048] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus.

[0049] This patent provides multiple parallel solutions; the different descriptions represent improved solutions or parallel solutions based on the basic solution. Each solution has its own unique characteristics. Furthermore, the technical features involved in the different embodiments of the invention described below can be combined with each other as long as they do not conflict with each other. Fixing methods not described herein can be any type of fixing, such as threaded fixing, bolt fixing, or adhesive bonding.

[0050] Example 1: A pure traditional Chinese medicine treatment for enterogenic mastitis in cattle and sheep, administered orally and applied topically, characterized in that...

[0051] The following oral and topical formulations are included in the following weight proportions:

[0052] The oral formulation is a feed additive for daily prevention.

[0053] The main ingredients of the oral medication formula are: dandelion (15-18 parts), salvia miltiorrhiza (17 parts), pomegranate peel extract (12 parts), and attapulgite (15 parts).

[0054] Oral adjuvants: Asparagus cochinchinensis (6-8 parts), Ligusticum chuanxiong (5-7 parts), Echinacea purpurea extract (5 parts) or Astragalus polysaccharide (5 parts);

[0055] Oral formulation of probiotic preparation: Lactobacillus plantarum: Lactobacillus acidophilus compound powder = 2:1 (15 parts);

[0056] The topical formulation is a local transdermal type, used for treatment during the onset of the disease;

[0057] The main ingredients of the external application formula are 48 parts: Prunella vulgaris (15 parts), Viola yedoensis (15 parts), Tetrapanax papyriferus (8 parts), Boswellia carterii (8 parts), and baicalin (2 parts).

[0058] External application formulation with 10 parts penetration enhancer: azone (4 parts), borneol (5.5 parts), menthol (0.5 parts)

[0059] 42 parts of the external application formulation carrier: ① Thermosensitive gel matrix (28 parts) (Poloxamer 407 + Poloxamer 188 = 3:1) ② Carbomer (1 part), curing temperature 33-37 degrees Celsius, curing into a film after contact with the breast (≤2 min), prolonging drug release to 8-12 hours. ③ Attapulgite (13 parts).

[0060] The substantive technical effects and implementation process of the technical solution presented herein, i.e., its basic functions, are as follows:

[0061] The project utilizes a "compound formula + microecology + gut-breast axis mechanism," employing a dual-pathway design of oral and topical administration to achieve dual intervention for mastitis from its source to its localized location. The oral formula contains pomegranate peel and attapulgite, which synergistically adsorb endotoxins and block enterogenic infection. The topical formula uses attapulgite gel carriers for sustained release and secretion clearance. The topical formula (local transdermal type) is used for treatment during the acute phase: its core principle is to rapidly reduce inflammation and swelling, block local breast infection, and promote tissue repair.

[0062] Main ingredients: Dandelion (clears heat and detoxifies, broad-spectrum antibacterial, regulates intestinal barrier), Salvia miltiorrhiza (anti-inflammatory and antioxidant, promotes blood circulation and removes blood stasis), pomegranate peel extract (astringent and antidiarrheal, adsorbs intestinal toxins), attapulgite (toxin adsorption / slow-release carrier);

[0063] Oral formulation adjuvants: Asparagus cochinchinensis + Ligusticum chuanxiong (immune regulation + blood circulation), Echinacea purpurea extract or Astragalus membranaceus polysaccharide (immune regulation); Lactobacillus plantarum: Lactobacillus acidophilus compound powder = 2:1 (probiotics colonize the intestines and inhibit pathogens); External application formulation main ingredients: Prunella vulgaris (dissipates nodules and reduces swelling), Viola yedoensis (broad-spectrum antibacterial, clears heat and detoxifies), Tetrapanax papyriferus, Boswellia carterii (synergistically improves mammary duct blockage), Baicalin (anti-inflammatory).

[0064] Additionally, menthol (for its cooling and analgesic effects) and wheat bran (which improves palatability and also acts as a prebiotic to regulate gut microbiota) are beneficial.

[0065] The in vitro antibacterial activity of extracts from traditional Chinese medicine formulas against four pathogenic bacteria causing mastitis in dairy cows was determined by the following method: Frozen strains of *Streptococcus agalactiae* and *Streptococcus dysgalactiae* were retrieved and streaked onto TSA (trypsin-soybean agar) + 5% sheep blood agar solid medium (121°C, sterilized for 20 minutes) for activation culture, and incubated at 37°C until single colonies formed. Single colonies were then inoculated onto brain and heart extract broth (BHI) liquid medium (121°C, sterilized for 20 minutes) and cultured at 37°C and 140 rpm on a shaker until the bacteria reached the logarithmic growth phase.

[0066] Similarly, frozen strains of Escherichia coli and Staphylococcus aureus were retrieved and activated by streaking on MH solid medium, and cultured at 37°C until single colonies formed. Single colonies were picked and inoculated into MH broth liquid medium (sterilized at 121°C for 20 minutes), and cultured at 37°C and 140 rpm on a shaker until the bacteria reached the logarithmic growth phase.

[0067] Finally, the four bacteria in the logarithmic growth phase were diluted with their respective liquid culture media to a cell colony count of approximately 10-1. 6 CFU / mL available for use.

[0068] The extracts of the traditional Chinese medicine formula were prepared into a drug-containing liquid culture medium with a concentration of 800 μg / mL. 50 μL of the drug-containing culture medium and the same volume of approximately 10... 6 CFU / mL bacterial culture was added to the wells of a 96-well plate to obtain a final drug concentration of 400 μg / mL. The 96-well plate was incubated at 37°C for 12–24 h until the control group bacterial culture grew. The OD value (OD) of the bacterial culture in the wells was measured using a microplate reader. 600 ).

[0069] The OD values ​​of 100 μL liquid culture medium and drug-containing culture medium were used as controls to correct the OD values ​​caused by the culture medium and the drug itself.

[0070] Corrected OD value = OD value of bacterial culture medium - OD value of sterile culture medium;

[0071]

[0072] The minimum mass concentration at which bacteria do not grow or inhibit bacterial growth by 90% is taken as the minimum inhibitory concentration (MIC) for different treatments against the bacteria.

[0073]

[0074] Clinical cure rate data table

[0075]

[0076] Example 2: As a further possible improvement, parallel solution, or optional independent solution, the external application formulation includes a kneading and dispensing system based on an improved breast pump;

[0077] The improved kneading and dispensing system based on the breast pump has an external thread 2 on the outer shell of the milk cup 1;

[0078] It also includes a rotating cup 3, which has internal threads inside;

[0079] The rotating cup 3 can be threaded onto the external thread 2, and rotating the rotating cup 3 can cause the rotating cup 3 to rise and fall.

[0080] A medication application structure 5 is connected to the rotating cup 3. The medication application structure 5 is a conical structure, and its inner wall 6 can adhere to the external medication. The substantive technical effect and implementation process of this technical solution, i.e., its basic function, are as follows: If the external medication application device can be combined with a milking machine, the problem of animal intolerance and non-cooperation can be solved, while reducing the danger and workload of manual labor. In this way, the medication can be applied directly to the udder while the animal is being milked, thus avoiding the difficulties and risks of applying medication separately. This device, which combines external medication application and milking functions, not only improves treatment efficiency but also reduces stress on the animal, making the entire treatment process more humane and efficient. When in use, the rotating cup 3 is rotated, and medication is applied while milking or while the animal is being milked. When not in use, the entire rotating cup is removed, just like the milk cup of a regular milking machine.

[0081] This minimizes modification costs and allows for full utilization of the animal's adaptive medication.

[0082] Example 3: As a further improvement, parallel, or optional independent solution, the inner wall 6 can adhere to release paper with external medication. The substantive technical effect and its implementation process, i.e., the basic function, of this technical solution is as follows: medication can be applied by peeling off the release paper.

[0083] Example 4: As a further improvement, parallel, or optional independent solution, the drug delivery structure 5 is made of rubber. The substantive technical effect and its implementation process, i.e., the basic function, of this technical solution are as follows: the rubber material allows for easy contact with the udder of cattle and sheep.

[0084] Example 5: As a further improvement, parallel, or optional independent solution, the medication delivery structure is an inflatable latex material. The substantive technical effect and its implementation process, i.e., the basic function, of this technical solution are as follows: After the latex material is inflated, medication can be easily applied. The inflatable latex material can conform to the udder area of ​​cattle and sheep after inflation.

[0085] Example 6: As a further improvement, parallel solution, or optional independent solution, it also includes an auxiliary infrared temperature sensor capable of measuring the temperature of the udder of cattle and sheep.

[0086] Innovatively, each of the above effects exists independently, yet a single structure can be used to combine the results.

[0087] It should be noted that the multiple solutions provided in this patent include their own basic solutions, are independent of each other, and do not restrict each other. However, they can also be combined with each other without conflict to achieve multiple effects simultaneously. The basic principles, main features, and advantages of the present invention have been shown and described above. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are only illustrative of the principles of the present invention. Various changes and modifications can be made to the present invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of protection claimed.

Claims

1. A pure traditional Chinese medicine treatment for enterogenic mastitis in cattle and sheep, administered orally and applied topically, characterized in that... The following oral and topical formulations are included in the following weight proportions: The oral formulation is a feed additive for daily prevention. Main ingredients of the oral medication formula: dandelion (15-18 parts), salvia miltiorrhiza (17 parts), pomegranate peel extract (12 parts), attapulgite (15 parts). Oral adjuvants: Asparagus cochinchinensis (6-8 parts), Ligusticum chuanxiong (5-7 parts), Echinacea purpurea extract (5 parts) or Astragalus polysaccharide (5 parts); Oral formulation of probiotic preparation: Lactobacillus plantarum: Lactobacillus acidophilus compound powder = 2:1 (15 parts); The topical formulation is a local transdermal type, used for treatment during the onset of the disease; The main ingredients of the external application formula are 48 parts: Prunella vulgaris (15 parts), Viola yedoensis (15 parts), Tetrapanax papyriferus (8 parts), Boswellia carterii (8 parts), and baicalin (2 parts). External application formulation with 10 parts penetration enhancer: azone (4 parts), borneol (5.5 parts), menthol (0.5 parts) 42 parts of the external application formulation carrier: ① Thermosensitive gel matrix (28 parts) (Poloxamer 407 + Poloxamer 188 = 3:1) ② Carbomer (1 part), curing temperature 33-37 degrees Celsius, after contact with the breast, it cures into a film (≤2 min), prolonging drug release to 8-12 hours. ③ Attapulgite (13 parts).

2. The pure traditional Chinese medicine for treating enterogenic mastitis in cattle and sheep as described in claim 1, characterized in that, The oral formulation also contains the following substances: Oral formulation of taste masking agent: 0.35 parts in total, including 0.2 parts steviol glycoside + 0.1 parts menthol + 0.05 parts sweetness-enhancing peptide, used to circumvent the bitterness threshold defect of single steviol glycoside.

3. The pure traditional Chinese medicine for treating enterogenic mastitis in cattle and sheep as described in claim 1, characterized in that, The oral formulation also contains the following substances: Carrier filler for the oral formulation: 8 parts wheat bran.

4. The pure traditional Chinese medicine for treating enterogenic mastitis in cattle and sheep as described in claim 1, characterized in that, The topical formulation includes a kneading and dispensing system based on an improved breast pump. The improved kneading and dosing system based on the breast pump has an external thread (2) on the outer shell of the milk cup (1). It also includes a rotating cup (3), which has internal threads inside; The rotating cup (3) can be threaded onto the external thread (2), and rotating the rotating cup (3) can drive the rotating cup (3) to rise and fall; The rotating cup (3) is connected to a drug delivery structure (5), which is a cone-shaped structure. The inner wall (6) of the drug delivery structure (5) can adhere to the external drug.

5. The pure traditional Chinese medicine for treating enterogenic mastitis in cattle and sheep as described in claim 4, characterized in that, The inner wall (6) can adhere to the release paper with the external medication.

6. The pure traditional Chinese medicine for treating enterogenic mastitis in cattle and sheep as described in claim 4, characterized in that, The dosing structure (5) is made of rubber.

7. The pure traditional Chinese medicine for treating enterogenic mastitis in cattle and sheep as described in claim 4, characterized in that, The dosing structure is made of an inflatable latex material.

8. The pure traditional Chinese medicine for treating enterogenic mastitis in cattle and sheep as described in claim 7, characterized in that, The inflatable latex material can conform to the udder area of ​​cattle and sheep after inflation.

9. The pure traditional Chinese medicine for treating enterogenic mastitis in cattle and sheep as described in claim 4, characterized in that, It also includes an auxiliary infrared temperature sensor that can measure the temperature of the udder of cattle and sheep.

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