Biological bin for sweet potato tissue culture, subculture and rapid propagation

Abstract

The invention discloses a biological bin for sweet potato tissue culture, subculture and rapid propagation, and relates to the technical field of plant tissue culture. The tissue culture device comprises a tissue culture frame, a tissue culture plate is placed in the tissue culture frame, a tissue culture tank is placed at the top of the tissue culture plate, a lifting mechanism is fixedly connected to one side of the tissue culture frame, and the lifting mechanism comprises a lifting plate fixedly connected to one side of the tissue culture frame and a lifting toothed plate fixedly connected to the interior of the lifting plate. Through the synergistic effect of the lifting mechanism and the monitoring mechanism, the monitoring box can freely move in the vertical direction and the horizontal direction, in the vertical direction, a double-shaft motor is meshed with a lifting toothed plate through a gear to drive the monitoring box to ascend and descend, in the horizontal direction, a threaded rod drives a threaded sleeve to transversely move, and a bidirectional camera covers a tissue culture frame; the movement process avoids manual bin opening intervention, ensures that the sterile environment is not damaged, realizes monitoring of the growth state of the tissue culture tank, and remarkably improves the monitoring efficiency and accuracy.

Description

Technical Field

[0001] This invention belongs to the field of plant tissue culture technology, and in particular relates to a bioreactor for rapid propagation of sweet potato tissue culture. Background Technology

[0002] Sweet potato tissue culture and rapid propagation is a method that utilizes the totipotency of plant cells to proliferate sweet potato explants in an artificially controlled environment through aseptic culture techniques. This technology mainly includes steps such as explant disinfection, primary culture, subculture propagation, and rooting and transplanting. It can achieve rapid, efficient, and disease-free propagation of sweet potato seedlings and is widely used in the production of virus-free seedlings, variety improvement, and germplasm resource preservation. A bio-bin is a closed or semi-closed environmental control system used for tissue culture. It is usually equipped with functional modules such as light, temperature and humidity regulation, CO2 supplementation, and aseptic operation area to provide optimal growth conditions. In sweet potato tissue culture, the core role of the bio-bin is to maintain a sterile environment while optimizing culture conditions to improve subculture efficiency and proliferation coefficient.

[0003] Existing bioreactors still have some problems during use, such as: difficulty in monitoring, the need to maintain strict aseptic conditions during tissue culture, the risk of contamination from frequent manual opening of the chamber for observation, which affects the success rate of culture, the difficulty in accurately and in real time to grasp the proliferation progress of different tissue culture racks due to reliance on manual recording of growth status, which leads to inconsistent subculture cycles or waste of resources, and the fact that existing monitoring methods are mostly fixed cameras, which are difficult to cover culture containers with multiple layers of tissue culture racks.

[0004] To address these issues, we provide a bioreactor for rapid propagation of sweet potato tissue culture. Summary of the Invention

[0005] The purpose of this invention is to provide a bioreactor for rapid propagation of sweet potato tissue culture. By coordinating a lifting mechanism, a monitoring mechanism, and a locking mechanism, it solves the problem in existing bioreactors where the existing monitoring methods, which are mostly fixed cameras, are difficult to cover the culture containers of multi-layer tissue culture racks.

[0006] To solve the above-mentioned technical problems, the present invention is achieved through the following technical solution.

[0007] This invention relates to a bioreactor for rapid propagation of sweet potato tissue culture, comprising a tissue culture rack, inside which a tissue culture plate is placed, and on top of the tissue culture plate a tissue culture tank. A lifting mechanism is fixedly connected to one side of the tissue culture rack, the lifting mechanism comprising a lifting plate fixedly connected to one side of the tissue culture rack, a lifting toothed plate fixedly connected inside the lifting plate, and a gear meshing on one side of the lifting toothed plate. A monitoring mechanism is provided on one side of the lifting plate, the monitoring mechanism comprising a monitoring box disposed on one side of the lifting plate, a screw movably connected to the monitoring box via a bearing, a threaded sleeve threaded to the surface of the screw, and a camera fixedly connected to the top of the threaded sleeve. A locking mechanism is provided on the other side of the monitoring box, the locking mechanism comprising a locking plate fixedly connected to the other side of the tissue culture rack, a locking toothed plate fixedly connected between the locking plate and the inside of the lifting plate, an anti-moving toothed plate meshing on one side of the locking toothed plate, and a hydraulic cylinder fixedly connected to one side of the anti-moving toothed plate.

[0008] The present invention is further configured such that a power distribution assembly is provided on one side of the monitoring box, the power distribution assembly including a dual-axis motor fixedly connected to one side of the monitoring box, a first electromagnetic clutch fixedly connected to one end of the dual-axis motor, and a second electromagnetic clutch fixedly connected to the other end of the dual-axis motor.

[0009] The present invention is further configured such that a synchronous pulley assembly is provided on one side of the dual-axis motor, the synchronous pulley assembly including a first synchronous pulley provided on one side of the dual-axis motor and a second synchronous pulley connected by a synchronous belt drive, the first synchronous pulley being fixedly connected to the second electromagnetic clutch.

[0010] The invention is further configured such that the top of the monitoring box has a movable through groove adapted to the screw sleeve, and the number of cameras is two, with the two cameras monitoring in opposite directions.

[0011] The present invention is further configured such that a limiting rod is fixedly connected to the bottom of the monitoring box, a limiting block is slidably connected to the surface of the limiting rod, and the top of the limiting block is fixedly connected to the bottom of the screw sleeve.

[0012] The invention is further configured such that sliders are fixedly connected to both the front and rear sides of the monitoring box, and vertical rods are slidably connected inside the sliders, with the number of vertical rods being four.

[0013] The invention is further configured such that the monitoring box is designed longitudinally, the monitoring box is located between the lifting plate and the locking plate, and the monitoring box is located between the left and right tissue culture racks.

[0014] The invention is further configured such that a fixing frame is fixedly connected to both the front and rear sides of the monitoring box, and the hydraulic cylinder is fixedly connected to one side of the fixing frame.

[0015] The invention is further configured such that a transmission groove is provided on one side of the monitoring box, and the transmission groove is located on the side close to the lifting plate.

[0016] The present invention is further configured such that the number of hydraulic cylinders is six, the number of anti-moving tooth plates and the number of locking tooth plates are three, and two hydraulic cylinders are fixedly connected to one side of the anti-moving tooth plates as a group.

[0017] The present invention has the following beneficial effects.

[0018] 1. This invention utilizes the synergistic effect of the lifting mechanism and the monitoring mechanism, allowing the monitoring box to move freely in both vertical and horizontal directions. In the vertical direction, a dual-axis motor drives the monitoring box to rise and fall through gear meshing with the lifting gear plate. In the horizontal direction, a screw drives the screw sleeve to move laterally, allowing the bidirectional camera to cover the tissue culture rack. This movement process avoids manual intervention by opening the chamber, ensuring that the sterile environment is not disrupted, while simultaneously enabling the monitoring of the growth status of the tissue culture tank, significantly improving monitoring efficiency and accuracy.

[0019] 2. In this invention, the locking mechanism uses a hydraulic cylinder to push the anti-moving toothed plate to engage with the locking toothed plate, firmly locking the monitoring box in the target position. Combined with the guiding effect of the limit rod and the slider, the monitoring box remains stable both when moving and stationary. This design effectively eliminates the offset of the camera caused by mechanical vibration or inertia, ensuring clear and stable image acquisition, providing a reliable basis for subsequent data analysis, and extending the service life of the equipment.

[0020] 3. The longitudinally designed monitoring box in this invention is located between two tissue culture racks. It monitors the cultures on both sides simultaneously through a bidirectional camera. The power distribution component switches the power direction through an electromagnetic clutch to achieve automated switching between vertical lifting and horizontal scanning. This structure is compact and rationally laid out, maximizing the use of the biological chamber space while reducing manual operation steps, significantly improving the level of intelligence and batch processing efficiency of tissue culture production.

[0021] Of course, any product implementing this invention does not necessarily need to achieve all of the advantages described above at the same time. Attached Figure Description

[0022] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below.

[0023] Figure 1 This is a three-dimensional diagram of a bioreactor used for rapid propagation of sweet potato tissue culture.

[0024] Figure 2 This is a structural diagram of the power distribution component in a bioreactor used for rapid propagation of sweet potato tissue culture.

[0025] Figure 3 This is a front view of a lifting mechanism in a bioreactor used for rapid propagation of sweet potato tissue culture.

[0026] Figure 4 This is a front view of a locking mechanism in a bioreactor used for rapid propagation of sweet potato tissue culture.

[0027] Figure 5 This is a front view of a monitoring mechanism in a bioreactor used for rapid propagation of sweet potato tissue culture.

[0028] Figure 6 This is a front view of a hydraulic cylinder and a fixing frame in a bioreactor used for rapid propagation of sweet potato tissue culture.

[0029] Figure 7 This is a front view of a limiting rod and limiting block in a biocompartment used for rapid propagation of sweet potato tissue culture.

[0030] Figure 8 This is a front view of the slider and vertical rod in a biocompartment used for rapid propagation of sweet potato tissue culture.

[0031] In the attached diagram: 1. Tissue culture rack; 2. Tissue culture plate; 3. Tissue culture tank; 4. Lifting mechanism; 401. Lifting plate; 402. Lifting toothed plate; 403. Gear; 5. Monitoring mechanism; 501. Monitoring box; 502. Screw; 503. Screw sleeve; 504. Camera; 6. Locking mechanism; 601. Locking plate; 602. Locking toothed plate; 603. Anti-movement toothed plate; 604. Hydraulic cylinder; 7. Power distribution assembly; 701. Dual-shaft motor; 702. First electromagnetic clutch; 703. Second electromagnetic clutch; 8. Synchronous pulley assembly; 801. First synchronous pulley; 802. Second synchronous pulley; 9. Limiting rod; 10. Limiting block; 11. Slider; 12. Vertical rod; 13. Fixing frame. Detailed Implementation

[0032] The technical solutions of the present invention will be described below with reference to the accompanying drawings. The described embodiments are only some embodiments of the present invention, and not all embodiments.

[0033] Example 1

[0034] Please see Figures 1-8This invention relates to a bioreactor for rapid propagation of sweet potato tissue culture, comprising a tissue culture rack 1, a tissue culture plate 2 placed inside the rack 1, a tissue culture tank 3 placed on top of the plate 2, a lifting mechanism 4 fixedly connected to one side of the rack 1, the lifting mechanism 4 including a lifting plate 401 fixedly connected to one side of the rack 1, a lifting toothed plate 402 fixedly connected inside the plate 401, and a gear 403 meshing with one side of the plate 402; and a monitoring mechanism 5 provided on one side of the lifting plate 401, the monitoring mechanism 5 including a monitoring box 50 disposed on one side of the plate 401. 1. A screw 502 is movably connected inside the monitoring box 501 via a bearing, a screw sleeve 503 is threaded onto the surface of the screw 502, and a camera 504 is fixedly connected to the top of the screw sleeve 503. A locking mechanism 6 is provided on the other side of the monitoring box 501. The locking mechanism 6 includes a locking plate 601 fixedly connected to the other side of the tissue culture rack 1, a locking toothed plate 602 fixedly connected to the locking plate 601 and the inside of the lifting plate 401, an anti-moving toothed plate 603 meshing with one side of the locking toothed plate 602, and a hydraulic cylinder 604 fixedly connected to one side of the anti-moving toothed plate 603.

[0035] Specifically: The lifting mechanism 4 is used to adjust the vertical height of the tissue culture rack 1. Gear 403 rotates under the drive of the dual-axis motor 701, meshing with the lifting gear plate 402 fixed inside the lifting plate 401, thereby pushing the lifting plate 401 to move up and down. The lifting plate 401 is fixedly connected to the tissue culture rack 1. The rotation of gear 403 is converted into linear motion of the lifting plate 401, driving the entire monitoring box 501 to move up and down, facilitating the monitoring of different levels of tissue culture tanks 3. The monitoring mechanism 5 is responsible for driving the camera 504 to move horizontally. The dual-axis motor 701 drives the screw 502 to rotate through the synchronous wheel assembly 8, and the screw sleeve 503 moves along the screw 502 horizontally. The camera moves horizontally, causing the top bidirectional camera 504 to monitor the tissue culture tanks 3 on the tissue culture rack 1. The limit rod 9 and the limit block 10 prevent the screw sleeve 503 from rotating, ensuring that the camera 504 moves in a straight line. After the camera 504 is adjusted to the specified layer height of the tissue culture rack 1, the monitoring box 501 is locked by the locking mechanism 6. The monitoring box 501 slides up and down along the vertical rod 12 via the slider 11, and rises and falls synchronously with the lifting mechanism 4 to achieve coverage monitoring of multiple layers of tissue culture rack 1. The hydraulic cylinder 604 pushes the anti-movement toothed plate 603 to engage with the locking toothed plate 602 to lock the monitoring box 501, preventing the camera 504 from shaking during monitoring and affecting the monitoring effect.

[0036] Example 2

[0037] Please see Figures 1-8Based on Embodiment 1, a power distribution assembly 7 is provided on one side of the monitoring box 501. The power distribution assembly 7 includes a dual-axis motor 701 fixedly connected to one side of the monitoring box 501, a first electromagnetic clutch 702 fixedly connected to one end of the dual-axis motor 701, and a second electromagnetic clutch 703 fixedly connected to the other end of the dual-axis motor 701. A synchronous pulley assembly 8 is provided on one side of the dual-axis motor 701. The synchronous pulley assembly 8 includes a first synchronous pulley 801 provided on one side of the dual-axis motor 701 and a second synchronous pulley 802 connected by a synchronous belt drive. The first synchronous pulley 801 is fixedly connected to the second electromagnetic clutch 703, and the gear 403 is connected to the first electromagnetic clutch 702. The clutch 702 is fixedly connected, the second synchronous pulley 802 is fixedly connected to the surface of the screw 502, the top of the monitoring box 501 is provided with a movable through groove that matches the screw sleeve 503, there are two cameras 504, the two cameras 504 monitor in opposite directions, the bottom of the monitoring box 501 is fixedly connected to a limit rod 9, the surface of the limit rod 9 is slidably connected to a limit block 10, the top of the limit block 10 is fixedly connected to the bottom of the screw sleeve 503, the front and rear sides of the monitoring box 501 are both fixedly connected to sliders 11, the inside of the sliders 11 is slidably connected to vertical rods 12, there are four vertical rods 12, the vertical rods 12 are in pairs and fixedly connected to the inside of the lifting plate 401 and the locking plate 601 respectively.

[0038] Specifically: the power distribution component 7 is used to control the power output direction of the dual-axis motor 701; the synchronous pulley component 8 is used to transmit the power from one end of the dual-axis motor 701 to the screw 502, causing the screw 502 to rotate; the two cameras 504 are used to monitor the left and right tissue culture racks 1; the limit rod 9 and the limit block 10 are used to limit the movement of the screw sleeve 503, preventing the screw sleeve 503 from rotating; the slider 11 and the vertical rod 12 are used to vertically limit the monitoring box 501, ensuring that the monitoring box 501 always moves along the axial direction of the vertical rod 12.

[0039] Example 3

[0040] Please see Figures 1-8 Based on Embodiments 1 and 2, the monitoring box 501 is designed longitudinally and is located between the lifting plate 401 and the locking plate 601. The monitoring box 501 is located between the two left and right tissue culture racks 1. The front and rear sides of the monitoring box 501 are fixedly connected to the fixing frame 13, which is L-shaped. The hydraulic cylinder 604 is fixedly connected to one side of the fixing frame 13. A transmission slot is opened on one side of the monitoring box 501, which is located near the lifting plate 401. There are six hydraulic cylinders 604. There are three anti-moving toothed plates 603 and three locking toothed plates 602. Two hydraulic cylinders 604 are fixedly connected to one side of the anti-moving toothed plate 603.

[0041] Specifically: The longitudinally designed monitoring box 501 is used to move two cameras 504 between the tissue culture racks 1 to monitor the left and right tissue culture racks 1. The fixed frame 13 is used to support the hydraulic cylinder 604. The transmission groove allows the synchronous belt to extend into the monitoring box 501 to complete the transmission between the first synchronous pulley 801 and the second synchronous pulley 802. The six hydraulic cylinders 604 provide sufficient driving force to the anti-moving toothed plate 603, so that the anti-moving toothed plate 603 and the locking toothed plate 602 are tightly engaged.

[0042] The working principle of this invention is as follows: the lifting mechanism 4 is driven by a dual-shaft motor 701. When the first electromagnetic clutch 702 is engaged, the power is transmitted to the gear 403. The gear 403 meshes with the lifting tooth plate 402 fixed inside the lifting plate 401, which drives the monitoring box 501 to rise and fall in the vertical direction. This design realizes the adjustment of the height of the monitoring box 501, so that the camera 504 can monitor the tissue culture tank 3 at different heights.

[0043] When horizontal monitoring is required, the second electromagnetic clutch 703 of the dual-axis motor 701 is engaged, and power is transmitted to the screw 502 through the synchronous pulley assembly 8. The rotation of the screw 502 drives the screw sleeve 503 to move horizontally, which in turn drives the bidirectional camera 504 fixed on its top to scan horizontally along the tissue culture rack 1. The limiting rod 9 and the limiting block 10 ensure that the screw sleeve 503 moves in a straight line and avoids deviation. The longitudinal design of the monitoring box 501 enables it to monitor the tissue culture racks 1 on both the left and right sides at the same time, which significantly improves the monitoring efficiency.

[0044] During the monitoring process, when the monitoring box 501 reaches the target height, the hydraulic cylinder 604 pushes the anti-movement toothed plate 603 to engage tightly with the locking toothed plate 602, firmly locking the monitoring box 501 in the current position. This locking mechanism effectively prevents the camera 504 from shaking due to vibration or movement during the monitoring process, ensuring the clarity and stability of image acquisition. After the monitoring is completed, the hydraulic cylinder 604 resets, releases the lock, and the monitoring box 501 can move freely to the next monitoring position.

[0045] The foregoing has only described certain exemplary embodiments of the present invention by way of illustration. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the foregoing drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A bioreactor for rapid propagation of sweet potato tissue culture, comprising a tissue culture rack (1), characterized in that: The tissue culture rack (1) contains a tissue culture plate (2), and a tissue culture tank (3) is placed on top of the tissue culture plate (2); The tissue culture rack (1) is fixedly connected to one side of a lifting mechanism (4). The lifting mechanism (4) includes a lifting plate (401) fixedly connected to one side of the tissue culture rack (1), a lifting tooth plate (402) fixedly connected inside the lifting plate (401), and a gear (403) meshing with one side of the lifting tooth plate (402). A monitoring mechanism (5) is provided on one side of the lifting plate (401). The monitoring mechanism (5) includes a monitoring box (501) provided on one side of the lifting plate (401), a screw (502) movably connected to the inside of the monitoring box (501) by a bearing, a screw sleeve (503) threadedly connected to the surface of the screw (502), and a camera (504) fixedly connected to the top of the screw sleeve (503). A locking mechanism (6) is provided on the other side of the monitoring box (501). The locking mechanism (6) includes a locking plate (601) fixedly connected to the other side of the tissue culture rack (1), a locking tooth plate (602) fixedly connected to the inside of the locking plate (601) and the lifting plate (401), an anti-moving tooth plate (603) meshing with one side of the locking tooth plate (602), and a hydraulic cylinder (604) fixedly connected to one side of the anti-moving tooth plate (603).

2. The bioreactor for rapid propagation of sweet potato tissue culture according to claim 1, characterized in that: A power distribution assembly (7) is provided on one side of the monitoring box (501). The power distribution assembly (7) includes a dual-axis motor (701) fixedly connected to one side of the monitoring box (501), a first electromagnetic clutch (702) fixedly connected to one end of the dual-axis motor (701), and a second electromagnetic clutch (703) fixedly connected to the other end of the dual-axis motor (701).

3. A bioreactor for rapid propagation of sweet potato tissue culture according to claim 2, characterized in that: A synchronous pulley assembly (8) is provided on one side of the dual-axis motor (701). The synchronous pulley assembly (8) includes a first synchronous pulley (801) provided on one side of the dual-axis motor (701) and a second synchronous pulley (802) connected by a synchronous belt drive. The first synchronous pulley (801) is fixedly connected to the second electromagnetic clutch (703).

4. A bioreactor for rapid propagation of sweet potato tissue culture according to claim 1, characterized in that: The top of the monitoring box (501) is provided with a movable through groove that is compatible with the screw sleeve (503). There are two cameras (504), and the two cameras (504) monitor in opposite directions.

5. A bioreactor for rapid propagation of sweet potato tissue culture according to claim 1, characterized in that: A limiting rod (9) is fixedly connected to the bottom of the monitoring box (501), and a limiting block (10) is slidably connected to the surface of the limiting rod (9). The top of the limiting block (10) is fixedly connected to the bottom of the screw sleeve (503).

6. A bioreactor for rapid propagation of sweet potato tissue culture according to claim 1, characterized in that: The monitoring box (501) is fixedly connected to the front and rear sides with sliders (11), and vertical rods (12) are slidably connected inside the sliders (11), and there are four vertical rods (12).

7. A bioreactor for rapid propagation of sweet potato tissue culture according to claim 1, characterized in that: The monitoring box (501) is designed vertically and is located between the lifting plate (401) and the locking plate (601). The monitoring box (501) is located between the left and right tissue culture racks (1).

8. A bioreactor for rapid propagation of sweet potato tissue culture according to claim 1, characterized in that: The monitoring box (501) is fixedly connected to a fixing frame (13) on both the front and rear sides, and the hydraulic cylinder (604) is fixedly connected to one side of the fixing frame (13).

9. A bioreactor for rapid propagation of sweet potato tissue culture according to claim 1, characterized in that: The monitoring box (501) has a transmission groove on one side, which is located near the lifting plate (401).

10. A bioreactor for rapid propagation of sweet potato tissue culture according to claim 1, characterized in that: The number of hydraulic cylinders (604) is six, the number of anti-moving toothed plates (603) and the number of locking toothed plates (602) are three each, and two hydraulic cylinders (604) are fixedly connected to one side of the anti-moving toothed plate (603) as a group.

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