Pesticide composition containing emamectin benzoate and application of pesticide composition in prevention and treatment of rice blast

Abstract

The invention discloses a pesticide composition containing emamectin benzoate and application of the pesticide composition in prevention and treatment of rice blast, and belongs to the technical field of pesticides. The pesticide composition comprises 1-5 parts of emamectin benzoate, 1-3 parts of cinnamon essential oil and 0.5-1.5 parts of orange peel essential oil. Adding polyvinyl alcohol into the heated deionized water, adding glycerol, and uniformly stirring to obtain a water phase; adding the pesticide composition into soybean oil, and then adding alkyl glycoside and phenolic ether phosphate to obtain an oil phase; under high-speed shearing, adding the oil phase into the water phase, continuously shearing, finally adding a defoaming agent, stirring and filtering to obtain the pesticide for preventing and treating the rice blast. The emamectin benzoate is compounded with the cinnamon essential oil and the orange peel essential oil, and the orange peel essential oil not only has an antibacterial effect, but also has a rapid permeation effect, so that permeation of the cinnamon essential oil can be accelerated, and volatilization of the cinnamon essential oil is reduced; components such as a film-forming agent are added into the auxiliary agent, so that a film is quickly formed after the pesticide is sprayed, and the volatilization of the cinnamon essential oil is further prevented; the control effect on the rice blast is obviously improved.

Description

Technical Field

[0001] This invention relates to the field of pesticide technology, specifically to pesticide compositions containing abamectin and their application in the control of rice blast. Background Technology

[0002] Emamectin benzoate, also known as abamectin, is not a completely synthetic chemical but rather derived from a natural product. Its parent compound, avermectin, is isolated from the fermentation products of the soil microorganism *Streptomyces avermitilis*. Emamectin benzoate is a chemically modified version of avermectin B1. By introducing a methylamino group, its biological activity (especially stomach poison toxicity) is increased by approximately 1-3 orders of magnitude compared to avermectin, while its toxicity to mammals is reduced, thus improving its safety. Emamectin benzoate is a neurotoxin that primarily acts on the nervous system of insect pests. Its mechanism of action involves stimulating the release of gamma-aminobutyric acid (GABA) in the pest. GABA binds tightly to GABA receptors, opening chloride ion channels and allowing a large influx of chloride ions into nerve cells. This blocks the normal transmission of nerve impulses, ultimately leading to paralysis, cessation of feeding, slowed movement, and death within 2-4 days.

[0003] Abamectin, a broad-spectrum pesticide, is most notably characterized by its extremely high activity against the larvae of lepidopteran pests, primarily controlling diamondback moth, cotton bollworm, and rice leaf roller. While mainly used for insect pest control, its application for disease control is less common. Patent application CN103503889A discloses a compound insecticidal and fungicidal composition, its formulation, and its application, using abamectin, hexaconazole, and jinggangmycin to kill and control rice pests and diseases. Although this composition can control rice blast, its control efficacy needs improvement.

[0004] Cinnamaldehyde, the main active ingredient in cinnamon essential oil, is a common natural antibacterial agent extracted from cinnamon. It possesses broad-spectrum antibacterial properties, exhibiting strong inhibitory effects against bacteria, molds, and yeasts. However, cinnamon essential oil is highly volatile, making it difficult to use in pesticides. Therefore, there is a need to design a pesticide composition containing abamectin for the control of rice blast. By combining abamectin and cinnamon essential oil, the volatility of cinnamon essential oil can be reduced, while still achieving highly effective control of rice blast. Summary of the Invention

[0005] In view of the above-mentioned prior art, the purpose of this invention is to provide a pesticide composition containing abamectin and its application in the control of rice blast. This invention uses abamectin in combination with cinnamon oil and orange peel oil. Orange peel oil not only has antibacterial properties but also rapid penetration, which can accelerate the penetration of cinnamon oil and reduce its volatilization. The adjuvants, by adding film-forming agents and other components, enable rapid film formation after pesticide spraying, further preventing the volatilization of cinnamon oil; thus significantly improving the control effect against rice blast.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: A first aspect of the present invention provides a pesticide composition containing abamectin, comprising the following raw materials in weight fractions: 1-5 parts abamectin, 1-3 parts cinnamon essential oil, and 0.5-1.5 parts orange peel essential oil.

[0007] Preferably, the raw materials include the following weight fractions: 3 parts abamectin, 1.2 parts cinnamon oil, and 0.8 parts orange peel oil.

[0008] In a second aspect, the present invention provides the application of the above-described pesticide composition in the control of rice blast.

[0009] In a third aspect, the present invention provides a pesticide for preventing and controlling rice blast, wherein the pesticide uses the above-mentioned pesticide composition as the active ingredient.

[0010] Preferably, the pesticide comprises the following raw materials in weight percentages: The pesticide composition consists of 1-10% pesticide, 10-30% adjuvants, and the remainder is water.

[0011] Preferably, the additives include film-forming matrix, glycerin, vegetable oil, surfactant, emulsifying stabilizer and defoamer.

[0012] Preferably, the film-forming matrix is ​​polyvinyl alcohol; the vegetable oil is soybean oil; the surfactant is alkyl glycoside; and the emulsifying stabilizer is phenolic ether phosphate.

[0013] Preferably, the polyvinyl alcohol accounts for 2.0% of the total mass of the pesticide; the glycerin accounts for 3.0% of the total mass of the pesticide; the soybean oil accounts for 10.0% of the total mass of the pesticide; the alkyl glycoside accounts for 2.5% of the total mass of the pesticide; the phenolic ether phosphate accounts for 1.0% of the total mass of the pesticide; and the defoamer accounts for 0.2% of the total mass of the pesticide.

[0014] Preferably, the pesticide is prepared by the following method: Polyvinyl alcohol was added to heated deionized water and kept warm while stirring until completely dissolved. Glycerin was added and stirred evenly to obtain an aqueous phase. The above pesticide composition was added to soybean oil and stirred. Alkyl glycosides and phenolic ether phosphates were then added and stirred evenly to obtain an oil phase. Under high-speed shearing, the oil phase was added to the aqueous phase and sheared continuously to form a stable primary emulsion. The primary emulsion was homogenized to obtain a stable emulsion. An antifoaming agent was added to the stable emulsion, and after stirring and filtration, a pesticide for controlling rice blast was obtained.

[0015] In a fourth aspect, the present invention provides a method for using the above-mentioned pesticide, characterized in that the method comprises: diluting the pesticide to 800-1500 times and then spraying it on the leaves at a rate of 1.0 L / mu.

[0016] The beneficial effects of this invention are: (1) This invention combines abamectin with cinnamon oil and orange peel oil, which significantly improves the control effect against rice blast. Furthermore, orange peel oil not only has antibacterial properties but also rapid penetration, which can accelerate the penetration of cinnamon oil and reduce its volatilization. The combination of these three agents can reduce the risk of pathogens developing resistance to a single agent and extend the lifespan of the agent.

[0017] (2) The pesticide for preventing rice blast prepared in this invention contains film-forming matrix, glycerin, vegetable oil, surfactant, emulsifying stabilizer and defoamer. After being sprayed on the leaves, it can help the pesticide form a film quickly, further preventing the volatilization of cinnamon essential oil. It can also reduce the total amount of pesticide in the environment by reducing the number of times it is applied, which is more in line with the development direction of green agriculture. Detailed Implementation

[0018] It should be noted that the following detailed descriptions are illustrative and intended to provide further explanation of this application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.

[0019] As described in the background section, cinnamaldehyde is the main active ingredient in cinnamon essential oil and a common natural antibacterial agent extracted from cinnamon. It has broad-spectrum antibacterial properties, exhibiting strong inhibitory effects against bacteria, molds, and yeasts. However, cinnamon essential oil is highly volatile, making it difficult to use in pesticides.

[0020] Based on this, the purpose of this invention is to provide a pesticide composition containing abamectin and its application in the control of rice blast. This invention discovered that combining abamectin and cinnamon oil can improve the control effect of rice blast, but cinnamon oil volatilizes too quickly, affecting the control effect when used as a pesticide. Therefore, this invention further investigated this by improving the permeability of the active ingredient and preventing its volatilization. It was found that adding orange peel oil not only improves the permeability of the active ingredient but also further enhances the control effect of rice blast. When abamectin and cinnamon oil are combined, the co-toxicity coefficient is significantly increased, showing a significant synergistic effect; while the addition of orange peel oil not only increases the co-toxicity coefficient, indicating that orange peel oil also improves the permeability of cinnamon oil. Regarding preventing the volatilization of the active ingredient, this invention, by adding adjuvants such as film-forming matrices, prepares a foliar spray pesticide that can quickly form a film, preventing the volatilization of cinnamon oil and further improving the control effect of rice blast. In addition, the pesticide of this invention can also increase rice yield.

[0021] To enable those skilled in the art to better understand the technical solution of this application, the technical solution of this application will be described in detail below with reference to specific embodiments.

[0022] The test materials used in the embodiments of this invention are all conventional test materials in the art and can be purchased through commercial channels.

[0023] Example 1: Indoor toxicity determination (co-toxicity coefficient method) Using *Magnaporthe oryzae*, the causal agent of rice blast, as the experimental subject, the mycelial growth rate method was employed. Different ratios of abamectin, cinnamon oil, and orange peel oil were added to PDA medium to prepare drug-containing plates. These plates were inoculated with cultured *Magnaporthe oryzae* cakes and incubated at 25℃ for 7 days. Colony diameters were measured, and the EC50 of each agent was calculated. 50 (Inhibition of medium concentration). Co-toxicity coefficient (CTC) was calculated using the Wadley method. The results are shown in Table 1.

[0024] Table 1 Indoor toxicity determination CTC>120 indicates a synergistic effect; 80≤CTC≤120 indicates an additive effect; CTC<80 indicates an antagonistic effect.

[0025] Table 1 shows that the co-toxicity coefficient of abamectin and cinnamon essential oil is 126.56, indicating a significant synergistic effect between the two. Compared with the addition of abamectin and cinnamon essential oil, the co-toxicity coefficient did not decrease after the addition of abamectin, cinnamon essential oil, and orange peel essential oil, indicating a synergistic effect among these three ingredients. Furthermore, the increase in the co-toxicity coefficient suggests that orange peel essential oil enhances the penetration of cinnamon essential oil into rice blast fungus, reduces the volatilization of cinnamon essential oil, and allows more cinnamon essential oil to exert its antibacterial effect.

[0026] Example 2 (1) Heat 76.3g of deionized water to 65℃, and slowly add 2g of polyvinyl alcohol (PVA-1788) while stirring. Keep warm and stir until completely dissolved. Add 3g of glycerin, stir evenly, and cool to room temperature to obtain the aqueous phase. Add 3g of abamectin, 1.2g of cinnamon essential oil, and 0.8g of orange peel essential oil to 10g of soybean oil and stir for 30min. Then add 2.5g of alkyl glycoside (APG-0810) and 1.0g of phenolic ether phosphate and continue stirring for 30min.

[0027] (2) Under high-speed shearing (12000 rpm), the oil phase was slowly added to the aqueous phase, and shearing was continued for 12 minutes to form a uniform and stable primary emulsion. The primary emulsion was homogenized three times using a high-pressure homogenizer (30 MPa) to obtain a stable emulsion. Finally, 0.2 g of organosilicon defoamer was added, and the mixture was stirred at low speed to defoam. The pesticide product was then filtered.

[0028] Comparative Example 1 The difference from Example 2 is that cinnamon oil, orange peel oil, and polyvinyl alcohol were not added, and the mixture was made up to 100g with deionized water. The final pesticide product was obtained.

[0029] Comparative Example 2 The difference from Example 2 is that orange peel oil and polyvinyl alcohol were not added, and the volume was increased to 100g with deionized water. The final pesticide product was obtained.

[0030] Comparative Example 3 The difference from Example 2 is that polyvinyl alcohol was not added, and deionized water was used to make up to 100g. The final pesticide product was obtained.

[0031] Test case Experimental location: Yaoqiang Town, Licheng District, Jinan City, Shandong Province; Rice variety: Maba Yinzhan.

[0032] Experimental groups: Example 2 group and Comparative Examples 1-3 groups. The pesticides prepared in Example 2 and Comparative Examples 1-3 were diluted 1000 times with water, with a dosage of 1.0 L per acre. An equal amount of water was used as a blank control group. One acre of rice was planted in each group, and the plots were divided into four plots (i.e., four replicates per group).

[0033] The pesticide was applied once each during the tillering and heading stages of rice. The incidence of rice blast in panicles was investigated six months after sowing, and the severity of the diseased panicles was graded. Three points were set up for each plot, with 100 panicles at each point. The yield was recorded after rice harvest, and the results are shown in Table 2.

[0034] Rice blast disease grading standards: Level 1: Individual branches and stalks are affected, with a loss of less than 5% per ear; Grade 2: 1 / 3 of the branches and stalks are affected, with a loss of about 20% per ear; Level 3: Disease occurs on the neck or main axis of the panicle, resulting in half-filled grains and a loss of about 50% per panicle; Level 4: Disease on the neck of the ear, many empty grains, loss of about 70% per ear; Level 5: Disease on the neck of the ear causes white ears, resulting in a loss of about 90% per ear.

[0035] Disease control efficacy (%) = (Disease rate in the control area - Disease rate in the treatment area) / Disease rate in the control area × 100%; Disease index = Σ (number of diseased ears (plants) at each level × representative value at each level) / (total number of ears (plants) surveyed × highest representative value) × 100; Disease control efficacy (%) = (Disease index in blank control area - Disease index in treatment area) / Disease index in blank control area × 100%.

[0036] Table 2. Rice Diseases and Yield As can be seen from Table 2, the disease index of Example 2 group was the lowest, the effect of controlling rice blast was the best, and the rice yield was the highest.

[0037] Compared with Comparative Example 1, Comparative Example 2 showed a significant increase in control efficacy after the addition of abamectin, indicating that abamectin and cinnamon essential oil have a synergistic effect, improving the control efficacy.

[0038] The addition of orange peel essential oil to Comparative Example 3 significantly improved the control effect, indicating that orange peel essential oil can not only enhance the control effect of rice blast, but also improve the permeability of cinnamon essential oil, thereby allowing more cinnamon essential oil to play its role.

[0039] Compared with Comparative Example 3, Example 2 showed improved yield and control effect, indicating that the film formed after pesticide spraying can further prevent the volatilization of cinnamon essential oil.

[0040] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A pesticide composition containing abamectin, characterized in that, Raw materials including the following weight fractions: 1-5 parts abamectin, 1-3 parts cinnamon essential oil, and 0.5-1.5 parts orange peel essential oil.

2. The pesticide composition containing abamectin according to claim 1, characterized in that, Raw materials including the following weight fractions: 3 parts abamectin, 1.2 parts cinnamon oil, and 0.8 parts orange peel oil.

3. The application of the pesticide composition according to claim 1 or 2 in the control of rice blast.

4. A pesticide for controlling rice blast, characterized in that, The pesticide uses the pesticide composition according to claim 1 or 2 as its active ingredient.

5. The pesticide according to claim 4, characterized in that, The pesticide comprises the following raw materials by weight percentage: The pesticide composition consists of 1-10% pesticide, 10-30% adjuvants, and the remainder is water.

6. The pesticide according to claim 5, characterized in that, The additives include film-forming matrix, glycerin, vegetable oil, surfactant, emulsifying stabilizer, and defoamer.

7. The pesticide according to claim 6, characterized in that, The film-forming matrix is ​​polyvinyl alcohol; the vegetable oil is soybean oil; the surfactant is alkyl glycoside; and the emulsifying stabilizer is phenolic ether phosphate.

8. The pesticide according to claim 7, characterized in that, The polyvinyl alcohol accounts for 2.0% of the total mass of the pesticide; the glycerin accounts for 3.0% of the total mass of the pesticide; the soybean oil accounts for 10.0% of the total mass of the pesticide; the alkyl glycoside accounts for 2.5% of the total mass of the pesticide; the phenolic ether phosphate accounts for 1.0% of the total mass of the pesticide; and the defoamer accounts for 0.2% of the total mass of the pesticide.

9. The pesticide according to claim 8, characterized in that, The pesticide is prepared by the following method: Polyvinyl alcohol is added to heated deionized water and kept warm while stirring until completely dissolved. Glycerin is added and stirred evenly to obtain an aqueous phase. The pesticide composition according to claim 1 or 2 is added to soybean oil and stirred. Alkyl glycoside and phenolic ether phosphate are then added and stirred evenly to obtain an oil phase. Under high-speed shearing, the oil phase is added to the aqueous phase and sheared continuously to form a stable primary emulsion. The primary emulsion is homogenized to obtain a stable emulsion. An antifoaming agent is added to the stable emulsion, and after stirring and filtration, a pesticide for controlling rice blast is obtained.

10. The method of using the pesticide according to any one of claims 4 to 9, characterized in that, The application method is as follows: dilute the pesticide to 800-1500 times and then spray it on the leaves at a rate of 1.0L / acre.

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