Natural fermented grease composition as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and particularly discloses a natural fermented grease composition and a preparation method and application thereof.According to the natural fermented grease composition, a probiotic compound fermentation preparation method is adopted, triglyceride is hydrolyzed into diglyceride or monoglyceride, meanwhile, grease contains rich probiotic metabolites and intracellular active ingredients, and therefore the natural fermented grease composition is prepared. The prepared composition has excellent permeability and good spreadability, and has the effects of moisturizing, repairing and oxidation resistance.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a natural fermented oil composition, its preparation method, and its application. Background Technology

[0002] The stratum corneum and sebum film, as the core structures of the skin barrier, are essentially a dynamic protective system built from a lipid network. Components in natural sebum, such as triglycerides and free fatty acids, work synergistically with ceramides and cholesterol between keratinocytes to form a "brick-and-mortar structure," maintaining the skin's ability to retain moisture and resist irritation. Oil-based ingredients in cosmetics, such as squalane and phytosterols, not only repair barrier function by mimicking or supplementing skin lipids, but their polarity also regulates the transdermal penetration efficiency of active ingredients.

[0003] In recent years, new cosmetic raw materials developed using microbial fermentation technology have attracted increasing attention. The advantage of microbial fermentation is that it can produce a variety of different ingredients. It can biosynthesize easily absorbed small molecules such as amino acids, organic acids, flavonoids, and polyphenols, as well as produce large molecules with important functions such as polysaccharides, polypeptides, or proteins. Many surfactants, preservatives, and other ingredients are also gradually being prepared using fermentation technology.

[0004] Therefore, this application is submitted. Summary of the Invention

[0005] This invention provides a natural fermented oil composition, its preparation method, and its application. The invention employs a probiotic compound fermentation method to hydrolyze triglycerides into diglycerides or monoglycerides. Simultaneously, the oil contains abundant probiotic metabolites and intracellular active ingredients, resulting in a composition with excellent permeability and spreadability, as well as moisturizing, repairing, and antioxidant effects.

[0006] The present invention solves its technical problem by adopting the following technical solution: A method for preparing a naturally fermented oil composition includes the following steps: (1) Mix rice bran oil, liquid avocado oil, and wheat germ oil evenly to obtain a mixed oil; (2) Add the fermentation culture medium to the mixed oil, mix evenly, sterilize, inoculate with Bacillus subtilis culture medium for aerobic fermentation, then add the inoculum culture medium for anaerobic fermentation, homogenize, centrifuge, collect the oil phase, add activated carbon for adsorption, filter, and obtain fermented combined oil; the inoculum culture medium includes brewer's yeast culture medium and Lactobacillus plantarum culture medium in a mass ratio of (1~2):(2~3); (3) Mix glycerol, polyglycerol-10 myristate, lecithin and water evenly, then mix with fermented combination oil evenly and homogenize to obtain natural fermented oil composition.

[0007] Rice bran is used in cosmetics primarily through two methods: direct application and the application of extracted active ingredients. Active ingredients extracted from rice bran, such as rice bran oil, oryzanol, phytosterols, octacosanol, vitamins, and phytic acid, can impart moisturizing, sun protection, anti-aging, antioxidant, and anti-wrinkle effects to cosmetics.

[0008] Wheat germ oil is rich in various bioactive components such as vitamin E, linoleic acid, linolenic acid, and octacosanol. Unsaturated fatty acids account for over 80% of its total content, with linoleic acid, which is crucial for the human body, accounting for over 60%. Its vitamin E content is the highest among many plant oils, containing all four types: α, β, γ, and δ. It is a complete form of vitamin E, with the most active α-type vitamin E reaching 60%. As a natural oil, wheat germ oil possesses excellent skin-friendly lipophilic and moisturizing properties, forming a film that provides both a superior feel and protection.

[0009] Shea butter contains abundant unsaponifiable components such as stearic acid, oleic acid, and linoleic acid, which have repairing, anti-inflammatory, and sun-protective effects. It can be widely used in skin creams, sunscreens, and hair products, becoming a safe and effective green additive in cosmetics.

[0010] This invention innovatively ferments a mixture of rice bran oil, wheat germ oil, and avocado fruit oil using Bacillus subtilis, Saccharomyces cerevisiae, and Lactobacillus plantarum. This fermented oil blend exhibits improved spreadability, a lighter texture, and superior absorption. Furthermore, the fermented oil blend is formulated into a natural fermented composition, preventing oil deterioration due to oxygen exposure and significantly enhancing its practicality.

[0011] As a preferred embodiment of the present invention, the mass ratio of rice bran oil, liquid avocado oil and wheat germ oil is (25~30):(1~2):(6~7).

[0012] As a preferred embodiment of the present invention, the fermentation culture medium comprises the following components by mass percentage: 1%~5% peptone, 1%~5% yeast extract, 1%~5% beef extract, 0.1%~0.5% NaCl, 0.1%~0.5% dipotassium hydrogen phosphate, and the balance being deionized water.

[0013] In a preferred embodiment of the present invention, the mass ratio of the fermentation culture medium to the mixed oil is (0.05~0.1):1; The mass ratio of the mixed oil, Bacillus subtilis culture medium, and bacterial culture medium is 1:(0.05~0.1):(0.05~0.1).

[0014] In a preferred embodiment of the present invention, the concentration of Bacillus subtilis in the Bacillus subtilis culture medium is 10. 8 ~109 cfu / mL; The concentration of Saccharomyces cerevisiae in the culture medium is 10. 8 ~10 9 cfu / mL; The concentration of *Lactobacillus plantarum* in the culture medium is 10. 8 ~10 9 cfu / mL.

[0015] In a preferred embodiment of the present invention, the temperature of the aerobic fermentation is 35~37℃ and the fermentation time is 18~30h; The anaerobic fermentation temperature is 28~30℃, and the fermentation time is 40~56h.

[0016] As a preferred embodiment of the present invention, the mass ratio of glycerol, polyglycerol-10 myristate, lecithin, water, and fermented oil is (30~35):(5~6):(0.2~0.3):(25~30):(38~40).

[0017] In a preferred embodiment of the present invention, the sterilization temperature is 115~120℃ and the time is 10~30min.

[0018] In a preferred embodiment of the present invention, the amount of activated carbon used is 0.5 to 2 wt% of the oil phase.

[0019] Among them, the Bacillus subtilis preservation number mentioned in this invention is CGMCC No.1107, the Saccharomyces cerevisiae preservation number is GDMCC 62898, and the Lactobacillus plantarum preservation number is ATCC NO.8014.

[0020] The Bacillus subtilis culture medium, Saccharomyces cerevisiae culture medium, and Lactobacillus plantarum culture medium are obtained by activating and amplifying Bacillus subtilis, Saccharomyces cerevisiae, and Lactobacillus plantarum, respectively.

[0021] The activation and scale-up culture process is as follows: slant tube activation → Erlenmeyer flask → seed tank. The composition and mass ratio of the culture medium for each strain are as follows: 1) Bacillus subtilis Slant culture medium: peptone 10g, yeast extract 5g, NaCl 10g, agar 20g, pH 7.0 Seed culture medium: peptone 10, glucose 5, beef extract 5, yeast powder 5, NaCl 5, pH 7.0.

[0022] Fermentation medium: yeast powder 6g, soluble starch 11g, glucose 6g, soybean meal 13.9g, beef extract 8.9g, corn flour 12.6g, pH 7.0.

[0023] 2) Brewing yeast Slant culture medium: peptone 2, glucose 2, yeast extract 1, agar 1, natural pH.

[0024] Seed culture medium: peptone 2, glucose 2, yeast extract 1, natural pH.

[0025] Fermentation medium: peptone 7.5%, glucose 20%, yeast extract 7.5%, dipotassium hydrogen phosphate 0.156%, magnesium sulfate heptahydrate 0.496%, pH 6.5.

[0026] 3) Lactobacillus plantarum Slant culture medium: peptone 1g, yeast extract 0.5g, NaCl 1g, agar 1g, pH 7.0.

[0027] Seed culture medium: peptone 1g, yeast extract 0.5g, NaCl 1g, pH 7.0.

[0028] Fermentation medium: peptone 10, glucose 20, yeast powder 5, beef extract 10, dipotassium hydrogen phosphate 2, magnesium sulfate heptahydrate 0.58, sodium acetate 5, diammonium citrate 2, manganese sulfate monohydrate 0.19, Tween 80 1, pH 6.5.

[0029] The liquid avocado oil mentioned in this application was purchased from AAK Sweden AB.

[0030] The rice bran oil and wheat germ oil mentioned in this application were purchased from Guangzhou Dingyue Biotechnology Co., Ltd.

[0031] The present invention also provides a natural fermented oil composition, which is prepared by the preparation method described above.

[0032] The present invention also provides an application of a naturally fermented oil composition in the preparation of cosmetics.

[0033] The present invention also provides a scented body butter lotion, comprising the following components by weight percentage: 0.2%–0.6% thickener, 0.01%–0.15% chelating agent, 0.1%–3% skin conditioning agent, 1%–3% emulsifier, 5%–15% emollient, 5%–15% moisturizer, 0.1%–0.3% pH adjuster, 0.5%–10% natural fermented oil composition, 0.01%–0.5% fragrance, 0.3%–1.5% preservative, and the balance being solvent; The natural fermentation composition is the natural fermentation oil composition described above.

[0034] The beneficial effects of the present invention are as follows: 1) The present invention adopts a probiotic compound fermentation preparation method to hydrolyze triglycerides into diglycerides or monoglycerides. At the same time, the oil contains rich probiotic metabolites and intracellular active ingredients, so that the prepared composition has excellent permeability and good spreadability, as well as moisturizing, repairing and antioxidant effects.

[0035] 2) This invention uses brewing yeast in conjunction with Lactobacillus plantarum for anaerobic fermentation. On the one hand, the ethanol produced by yeast and the lactic acid produced by Lactobacillus plantarum together form an antibacterial environment, which inhibits the growth of putrefactive and pathogenic bacteria and delays the rancidity of the fermented oil. On the other hand, the glutathione and polyphenols secreted by yeast and the organic acids secreted by Lactobacillus plantarum neutralize free radicals, reduce oxidation reactions, and extend the shelf life of the fermented oil.

[0036] 3) The combination oils selected in this invention have improved emulsification ability after fermentation, and can achieve excellent emulsification effect with less emulsifier, greatly reducing skin irritation. Detailed Implementation

[0037] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0038] In this invention, the technical features described in an open-ended manner include both closed-ended technical solutions composed of the listed features and open-ended technical solutions that include the listed features.

[0039] In this invention, numerical ranges are involved. Unless otherwise specified, the numerical ranges are considered continuous and include the minimum and maximum values ​​of the range, as well as every value between the minimum and maximum values. Furthermore, when the range refers to integers, it includes every integer between the minimum and maximum values ​​of the range. Additionally, when multiple ranges are provided to describe features or characteristics, the ranges may be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges to which they are included.

[0040] In this invention, there are no particular limitations on the specific dispersion and stirring methods.

[0041] Unless otherwise specified, all reagents or instruments used in this invention are commercially available conventional products. Unless otherwise specified, the raw materials used in each comparative example and the parallel experiments of each embodiment are the same commercially available products.

[0042] Example 1 Activation and scale-up culture of three bacterial strains 1) Inoculate Bacillus subtilis into sterile slant culture medium (peptone 10, yeast extract 5, NaCl 10, agar 20, pH 7.0) and incubate at 37℃ for 24 h. Pick a loopful of Bacillus subtilis from the slant and inoculate it into sterile seed culture medium (peptone 10, glucose 5, beef extract 5, yeast extract 5, NaCl 5, pH 7.0), and incubate at 37℃ and 180 rpm on a shaker for 10 h. Then, transfer the 7% inoculum to a 250 mL shake flask containing 30 mL of sterile fermentation medium (yeast extract 6, soluble starch 11, glucose 6, soybean meal 13.9, beef extract 8.9, corn flour 12.6, pH 7.0) and ferment at 37℃ and 180 rpm. The seed culture was inoculated into a seed tank containing sterile fermentation medium, filling it to 70% capacity. The tank was incubated at 37°C with approximately 20% dissolved oxygen. After 5 hours of fermentation, 50% glucose was continuously added to maintain a glucose concentration of 3.0–6.0 g / L in the fermentation broth. During fermentation, the pH was adjusted to 7.0 using NaOH. Feeding was stopped after 20 hours, and fermentation was halted after 40 hours. The culture was centrifuged, and the supernatant was collected. Deionized water was added to adjust the concentration of Bacillus subtilis in the culture medium to 7.2 × 10⁻⁶. 8 CFU / mL.

[0043] 2) Inoculate the *Saccharomyces cerevisiae* into sterile slant culture medium (peptone 2, glucose 2, yeast extract 1, agar 1, natural pH) and incubate at 28°C for 36 h. Take a loopful of *Saccharomyces cerevisiae* from the slant and inoculate it into sterile seed culture medium (peptone 2, glucose 2, yeast extract 1, natural pH), and incubate at 28°C and 250 rpm on a shaker for 30 h. Transfer the 8% inoculum to a 250 mL shake flask containing 30 mL of sterile fermentation medium (peptone 7.5, glucose 20, yeast extract 7.5, dipotassium hydrogen phosphate 0.156, magnesium sulfate heptahydrate 0.496, pH 6.5), and ferment at 28°C and 250 rpm. Inoculate the seed culture into a seed tank containing sterile fermentation medium, filling to 20% capacity. After 30 h, stop fermentation, centrifuge, collect the supernatant, and add deionized water to adjust the concentration of *Saccharomyces cerevisiae* in the culture medium to 1 × 10⁻⁶. 8 CFU / mL.

[0044] 3) Inoculate *Lactobacillus plantarum* into sterile slant culture medium (1 part peptone, 0.5 part yeast extract, 1 part NaCl, 1 part agar, pH 7.0) and incubate at 37°C for 14 h. Pick a loopful of *Lactobacillus plantarum* from the slant and inoculate it into sterile seed culture medium (1 part peptone, 0.5 part yeast extract, 1 part NaCl, pH 7.0). Incubate at 37°C and 180 rpm on a shaker for 24 h. Transfer the 5% inoculum to a 250 mL shake flask containing 30 mL of sterile fermentation medium (10 parts peptone, 20 parts glucose, 5 parts yeast extract, 10 parts beef extract, 2 parts dipotassium hydrogen phosphate, 0.58 g magnesium sulfate heptahydrate, 5 parts sodium acetate, 2 g diammonium citrate, 0.19 g manganese sulfate monohydrate, 1 part Tween 80, pH 7.0) and ferment at 37°C and 180 rpm. The seed culture was inoculated into a seed tank containing sterile fermentation medium, with a liquid volume of 20%. Fermentation was stopped after 24 hours, centrifuged, and the supernatant was collected. Deionized water was added to adjust the concentration of *Lactobacillus plantarum* in the culture medium to 5 × 10⁻⁶. 8 CFU / mL.

[0045] Example 2 A method for preparing a natural fermented oil composition includes the following steps: (1) Pretreatment of fermentation substrate: Mix rice bran oil, liquid avocado oil and wheat germ oil in a ratio of 30:2:6.5 and stir evenly to obtain mixed oil.

[0046] (2) Add 3kg of mixed oil to a 5L fermenter, turn on the stirrer at 180rpm, add 0.3kg of fermentation culture medium, sterilize at 116℃ for 20min, cool to 37℃, then add 0.3kg of Bacillus subtilis culture medium from Example 1, introduce sterile air for aerobic fermentation, fermentation time is 24h, and fermentation product 1 is obtained.

[0047] The fermentation broth comprises 15g peptone, 15g yeast powder, 15g beef extract, 1.5g NaCl, 1.5g dipotassium hydrogen phosphate, and 255g deionized water, with a pH of 6.5.

[0048] (3) When the temperature drops to 30°C, add 0.3 kg of the brewing yeast culture and plant lactobacillus culture of Example 1 by mass ratio of 1:2 and carry out anaerobic fermentation for 48 hours to obtain fermentation product 2.

[0049] (4) Take out fermentation product 2, lower the temperature to 4℃, put it into a high-speed homogenizer for high-pressure homogenization at a pressure of 30MPa, and cycle 3 times. Then centrifuge the fermentation product at 9000rpm for 20min, collect the oil phase, heat it to 55℃, adsorb it with 1wt% activated carbon in the oil phase for 1h, and then filter it through a 1.2μm fine filter plate and a 0.22μm filter screen to obtain the fermented composite oil.

[0050] (5) a. Aqueous phase: Mix 30 parts glycerol, 6 parts polyglycerol-10 myristate, 0.3 parts lecithin and 25.2 parts water at 80°C until homogeneous, and rotate at 30 rpm. b. Oil phase: 38.5 parts of fermented blend oil were stirred at 30 rpm at 80°C; c. Slowly add the oil phase to the water phase, homogenize at high speed (3000 rpm) for 6 minutes, stir for 20 minutes, cool down and filter with a 300 mesh to obtain the natural fermentation composition.

[0051] The high-speed homogenizer was purchased from Niro Soavi, Italy, model NS1001L Panda 2K.

[0052] (6) Prepare the scented body butter lotion according to the following proportions: 0.33% Acrylic (ester) compounds / C10-30 alkanol acrylate crosspolymer, 0.03% Disodium EDTA, 0.2% Allantoin, 3% Glycerin, 4% Glyceryl polyether-26, 0.04% Sodium hyaluronate, 0.03% Sodium polyglutamate, 1.5% Glyceryl mono- and glyceryl distearate, 0.5% Olive oil polyglycerol-6 esters, 2% Ethylhexyl stearate, 0.5% Dimethicone, 2% Isohexadecane, 5% Mineral oil, 0.15% Arginine, 0.5% p-Hydroxyacetophenone, 0.5% 1,2-Hexanediol, 0.7% Gentian extract, 0.3% Hydroxypropyl tetrahydropyrantriol, 0.05% Collagen, 7% Natural fermentation composition, 0.4% Fragrance, Balance: Water.

[0053] The preparation steps for the scented body butter lotion are as follows: a. First, add deionized water to the emulsifying pot, start homogenization at 3000 rpm, add phase A1 (0.33% acrylate / C10-30 alkanol acrylate crosspolymer), phase A2 (0.2% allantoin, 3% glycerol), and phase A3 (4% glycerol polyether-26, 0.04% sodium hyaluronate, 0.03% sodium polyglutamate) (phase A3 is pre-mixed evenly), start stirring at 30 rpm, high-speed homogenization (3000 rpm) for 6 minutes, then heat to 82℃.

[0054] b. Add phase B (1.5% mono- and di-stearate glycerides, 0.5% olive oil polyglycerol-6 esters, 2% ethylhexyl stearate, 0.5% polydimethylsiloxane, 2% isohexadecane, 5% mineral oil) to a pot of oil and heat to 81°C.

[0055] c. Draw phase B into the emulsification pot, vacuum it to -0.03 MPa, homogenize it at high speed (3000 rpm) for 6 minutes, then add phase C (0.15% arginine), vacuum it to -0.03 MPa, homogenize it at high speed (3000 rpm) for 6 minutes, keep it warm for 20 minutes, and then cool it down.

[0056] d. After cooling to 40-45℃, add phase D (0.7% gentian extract, 0.3% hydroxypropyl tetrahydropyrantriol, 0.05% collagen, 7% natural fermentation composition, 0.4% fragrance) and phase E (0.5% p-hydroxyacetophenone, 0.5% 1,2-hexanediol) (phase E ingredients need to be heated and dissolved until transparent before adding). Adjust the speed to 25 rpm and stir continuously for 20 minutes.

[0057] The mono- and di-stearyl glycerides were purchased from Hangzhou Oil & Fat Chemical Co., Ltd.

[0058] Example 3 A method for preparing a natural fermented oil composition includes the following steps: (1) Pretreatment of fermentation substrate: Mix rice bran oil, liquid avocado oil and wheat germ oil in a ratio of 25:1:7 and stir evenly to obtain mixed oil.

[0059] (2) Add 3kg of mixed oil to a 5L fermenter, turn on the stirrer at 180rpm, add 0.3kg of fermentation culture medium, sterilize at 116℃ for 20min, cool to 37℃, then add 0.3kg of Bacillus subtilis culture medium from Example 1, introduce sterile air for aerobic fermentation, fermentation time is 24h, and fermentation product 1 is obtained.

[0060] The fermentation broth comprises 15g peptone, 15g yeast powder, 15g beef extract, 1.5g NaCl, 1.5g dipotassium hydrogen phosphate, and 255g deionized water, with a pH of 6.5.

[0061] (3) When the temperature drops to 30°C, add 0.3 kg of the brewing yeast culture and plant lactobacillus culture of Example 1 by mass ratio of 1:2 and carry out anaerobic fermentation for 48 hours to obtain fermentation product 2.

[0062] (4) Take out fermentation product 2, lower the temperature to 4℃, put it into a high-speed homogenizer for high-pressure homogenization at a pressure of 30MPa, and cycle 3 times. Then centrifuge the fermentation product at 9000rpm for 20min, collect the oil phase, heat it to 55℃, adsorb it with 1wt% activated carbon in the oil phase for 1h, and then filter it through a 1.2μm fine filter plate and a 0.22μm filter screen to obtain the fermented composite oil.

[0063] (5) a. Aqueous phase: Mix 30 parts glycerol, 6 parts polyglycerol-10 myristate, 0.3 parts lecithin and 25.2 parts water at 80°C until homogeneous, and rotate at 30 rpm. b. Oil phase: 38.5 parts of fermented blend oil were stirred at 30 rpm at 80°C; c. Slowly add the oil phase to the water phase, homogenize at high speed (3000 rpm) for 6 minutes, stir for 20 minutes, cool down and filter with a 300 mesh to obtain the natural fermentation composition.

[0064] The high-speed homogenizer was purchased from Niro Soavi, Italy, model NS1001L Panda 2K.

[0065] (6) Prepare the scented body butter lotion according to the following proportions: 0.33% Acrylic (ester) compounds / C10-30 alkanol acrylate crosspolymer, 0.03% Disodium EDTA, 0.2% Allantoin, 3% Glycerin, 4% Glyceryl polyether-26, 0.04% Sodium hyaluronate, 0.03% Sodium polyglutamate, 1.5% Glyceryl mono- and glyceryl distearate, 0.5% Olive oil polyglycerol-6 esters, 2% Ethylhexyl stearate, 0.5% Dimethicone, 2% Isohexadecane, 5% Mineral oil, 0.15% Arginine, 0.5% p-Hydroxyacetophenone, 0.5% 1,2-Hexanediol, 0.7% Gentian extract, 0.3% Hydroxypropyl tetrahydropyrantriol, 0.05% Collagen, 7% Natural fermentation composition, 0.4% Fragrance, Balance: Water.

[0066] Example 4 A method for preparing a natural fermented oil composition includes the following steps: (1) Pretreatment of fermentation substrate: Mix rice bran oil, liquid avocado oil and wheat germ oil in a ratio of 30:2:6.5 and stir evenly to obtain mixed oil.

[0067] (2) Add 3kg of mixed oil to a 5L fermenter, turn on the stirrer at 180rpm, add 0.3kg of fermentation culture medium, sterilize at 116℃ for 20min, cool to 37℃, then add 0.3kg of Bacillus subtilis culture medium from Example 1, introduce sterile air for aerobic fermentation, fermentation time is 24h, and fermentation product 1 is obtained.

[0068] The fermentation broth comprises 15g peptone, 15g yeast powder, 15g beef extract, 1.5g NaCl, 1.5g dipotassium hydrogen phosphate, and 255g deionized water, with a pH of 6.5.

[0069] (3) When the temperature drops to 30°C, add 0.3 kg of the brewing yeast culture and plant lactobacillus culture of Example 1 by mass ratio of 1:2 and carry out anaerobic fermentation for 48 hours to obtain fermentation product 2.

[0070] (4) Take out fermentation product 2, lower the temperature to 4℃, put it into a high-speed homogenizer for high-pressure homogenization at a pressure of 30MPa, and cycle 3 times. Then centrifuge the fermentation product at 9000rpm for 20min, collect the oil phase, heat it to 55℃, adsorb it with 1wt% activated carbon in the oil phase for 1h, and then filter it through a 1.2μm fine filter plate and a 0.22μm filter screen to obtain the fermented composite oil.

[0071] (5) a. Aqueous phase: Mix 35 parts glycerol, 5 parts polyglycerol-10 myristate, 0.2 parts lecithin and 30 parts water at 80°C until homogeneous, and rotate at 30 rpm. b. Oil phase: Stir 40 parts of fermented oil mixture at 80°C at 30 rpm; c. Slowly add the oil phase to the water phase, homogenize at high speed (3000 rpm) for 6 minutes, stir for 20 minutes, cool down and filter with a 300 mesh to obtain the natural fermentation composition.

[0072] The high-speed homogenizer was purchased from Niro Soavi, Italy, model NS1001L Panda 2K.

[0073] (6) Prepare the scented body butter lotion according to the following proportions: 0.33% Acrylic (ester) compounds / C10-30 alkanol acrylate crosspolymer, 0.03% Disodium EDTA, 0.2% Allantoin, 3% Glycerin, 4% Glyceryl polyether-26, 0.04% Sodium hyaluronate, 0.03% Sodium polyglutamate, 1.5% Glyceryl mono- and glyceryl distearate, 0.5% Olive oil polyglycerol-6 esters, 2% Ethylhexyl stearate, 0.5% Dimethicone, 2% Isohexadecane, 5% Mineral oil, 0.15% Arginine, 0.5% p-Hydroxyacetophenone, 0.5% 1,2-Hexanediol, 0.7% Gentian extract, 0.3% Hydroxypropyl tetrahydropyrantriol, 0.05% Collagen, 7% Natural fermentation composition, 0.4% Fragrance, Balance: Water.

[0074] Comparative Example 1

[0075] The difference between Comparative Example 1 and Example 2 is that in the scented body butter lotion of Comparative Example 1, an equal amount of mixed oil was used to replace the natural fermented composition.

[0076] Mix rice bran oil, liquid avocado oil, and wheat germ oil in a ratio of 30:2:6.5 and stir well to obtain a mixed oil.

[0077] Comparative Example 2

[0078] A method for preparing a natural fermented oil composition includes the following steps: (1) Pretreatment of fermentation substrate: Mix rice bran oil, liquid avocado oil and wheat germ oil in a ratio of 30:2:6.5 and stir evenly to obtain mixed oil.

[0079] (2) Add 3kg of mixed oil to a 5L fermenter, turn on the stirrer at 180rpm, add 0.3kg of fermentation culture medium, sterilize at 116℃ for 20min, cool to 37℃, then add 0.3kg of Bacillus subtilis culture medium from Example 1, introduce sterile air for aerobic fermentation, fermentation time is 24h, and fermentation product 1 is obtained.

[0080] The fermentation broth comprises 15g peptone, 15g yeast powder, 15g beef extract, 1.5g NaCl, 1.5g dipotassium hydrogen phosphate, and 255g deionized water, with a pH of 6.5.

[0081] (3) Take out fermentation product 1, lower the temperature to 4℃, put it into a high-speed homogenizer for high-pressure homogenization at 30MPa, and cycle 3 times. Then centrifuge the fermentation product at 9000rpm for 20min, collect the oil phase, heat it to 55℃, adsorb it with 1wt% activated carbon in the oil phase for 1h, and then filter it through a 1.2μm fine filter plate and a 0.22μm filter screen to obtain the fermentation combination oil.

[0082] (4) a. Aqueous phase: Mix 30 parts glycerol, 6 parts polyglycerol-10 myristate, 0.3 parts lecithin and 25.2 parts water at 80°C until homogeneous, and rotate at 30 rpm. b. Oil phase: 38.5 parts of fermented blend oil were stirred at 30 rpm at 80°C; c. Slowly add the oil phase to the water phase, homogenize at high speed (3000 rpm) for 6 minutes, stir for 20 minutes, cool down and filter with a 300 mesh to obtain the natural fermentation composition.

[0083] The high-speed homogenizer was purchased from Niro Soavi, Italy, model NS1001L Panda 2K.

[0084] (5) Prepare the scented body butter lotion according to the following proportions: 0.33% Acrylic (ester) compounds / C10-30 alkanol acrylate crosspolymer, 0.03% Disodium EDTA, 0.2% Allantoin, 3% Glycerin, 4% Glyceryl polyether-26, 0.04% Sodium hyaluronate, 0.03% Sodium polyglutamate, 1.5% Glyceryl mono- and glyceryl distearate, 0.5% Olive oil polyglycerol-6 esters, 2% Ethylhexyl stearate, 0.5% Dimethicone, 2% Isohexadecane, 5% Mineral oil, 0.15% Arginine, 0.5% p-Hydroxyacetophenone, 0.5% 1,2-Hexanediol, 0.7% Gentian extract, 0.3% Hydroxypropyl tetrahydropyrantriol, 0.05% Collagen, 7% Natural fermentation composition, 0.4% Fragrance, Balance: Water.

[0085] Comparative Example 3

[0086] A method for preparing a natural fermented oil composition includes the following steps: (1) Pretreatment of fermentation substrate: Mix rice bran oil, liquid avocado oil and wheat germ oil in a ratio of 30:2:6.5 and stir evenly to obtain mixed oil.

[0087] (2) Add 3 kg of mixed oil to a 5L fermenter, turn on the stirrer at 180 rpm, add 0.3 kg of fermentation culture medium, sterilize at 116℃ for 20 min, cool to 37℃, and then add 0.3 kg of brewer's yeast culture medium and Lactobacillus plantarum culture medium from Example 1. The mass ratio of the two cultures is 1:2. Perform anaerobic fermentation for 48 h to obtain fermentation product 2.

[0088] (3) Take out fermentation product 2, lower the temperature to 4℃, put it into a high-speed homogenizer for high-pressure homogenization at 30MPa, and cycle 3 times. Then centrifuge the fermentation product at 9000rpm for 20min, collect the oil phase, heat it to 55℃, adsorb it with 1wt% activated carbon in the oil phase for 1h, and then filter it through a 1.2μm fine filter plate and a 0.22μm filter screen to obtain the fermented composite oil.

[0089] (4) a. Aqueous phase: Mix 30 parts glycerol, 6 parts polyglycerol-10 myristate, 0.3 parts lecithin and 25.2 parts water at 80°C until homogeneous, and rotate at 30 rpm. b. Oil phase: 38.5 parts of fermented blend oil were stirred at 30 rpm at 80°C; c. Slowly add the oil phase to the water phase, homogenize at high speed (3000 rpm) for 6 minutes, stir for 20 minutes, cool down and filter with a 300 mesh to obtain the natural fermentation composition.

[0090] The high-speed homogenizer was purchased from Niro Soavi, Italy, model NS1001L Panda 2K.

[0091] (5) Prepare the scented body butter lotion according to the following proportions: 0.33% Acrylic (ester) compounds / C10-30 alkanol acrylate crosspolymer, 0.03% Disodium EDTA, 0.2% Allantoin, 3% Glycerin, 4% Glyceryl polyether-26, 0.04% Sodium hyaluronate, 0.03% Sodium polyglutamate, 1.5% Glyceryl mono- and glyceryl distearate, 0.5% Olive oil polyglycerol-6 esters, 2% Ethylhexyl stearate, 0.5% Dimethicone, 2% Isohexadecane, 5% Mineral oil, 0.15% Arginine, 0.5% p-Hydroxyacetophenone, 0.5% 1,2-Hexanediol, 0.7% Gentian extract, 0.3% Hydroxypropyl tetrahydropyrantriol, 0.05% Collagen, 7% Natural fermentation composition, 0.4% Fragrance, Balance: Water.

[0092] Comparative Example 4

[0093] A method for preparing a natural fermented oil composition includes the following steps: (1) Pretreatment of fermentation substrate: Mix rice bran oil, liquid avocado oil and wheat germ oil in a ratio of 30:2:6.5 and stir evenly to obtain mixed oil.

[0094] (2) Add 3kg of mixed oil to a 5L fermenter, turn on the stirrer at 180rpm, add 0.3kg of fermentation culture medium, sterilize at 116℃ for 20min, cool to 37℃, then add 0.3kg of Bacillus subtilis culture medium from Example 1, introduce sterile air for aerobic fermentation, fermentation time is 24h, and fermentation product 1 is obtained.

[0095] The fermentation broth comprises 15g peptone, 15g yeast powder, 15g beef extract, 1.5g NaCl, 1.5g dipotassium hydrogen phosphate, and 255g deionized water, with a pH of 6.5.

[0096] (3) When the temperature drops to 30°C, 0.3 kg of the brewing yeast culture broth from Example 1 is added at a mass ratio for anaerobic fermentation. The fermentation time is 48 h, and fermentation product 2 is obtained.

[0097] (4) Take out fermentation product 2, lower the temperature to 4℃, put it into a high-speed homogenizer for high-pressure homogenization at a pressure of 30MPa, and cycle 3 times. Then centrifuge the fermentation product at 9000rpm for 20min, collect the oil phase, heat it to 55℃, adsorb it with 1wt% activated carbon in the oil phase for 1h, and then filter it through a 1.2μm fine filter plate and a 0.22μm filter screen to obtain the fermented composite oil.

[0098] (5) a. Aqueous phase: Mix 30 parts glycerol, 6 parts polyglycerol-10 myristate, 0.3 parts lecithin and 25.2 parts water at 80°C until homogeneous, and rotate at 30 rpm. b. Oil phase: 38.5 parts of fermented blend oil were stirred at 30 rpm at 80°C; c. Slowly add the oil phase to the water phase, homogenize at high speed (3000 rpm) for 6 minutes, stir for 20 minutes, cool down and filter with a 300 mesh to obtain the natural fermentation composition.

[0099] The high-speed homogenizer was purchased from Niro Soavi, Italy, model NS1001L Panda 2K.

[0100] (6) Prepare the scented body butter lotion according to the following proportions: 0.33% Acrylic (ester) compounds / C10-30 alkanol acrylate crosspolymer, 0.03% Disodium EDTA, 0.2% Allantoin, 3% Glycerin, 4% Glyceryl polyether-26, 0.04% Sodium hyaluronate, 0.03% Sodium polyglutamate, 1.5% Glyceryl mono- and glyceryl distearate, 0.5% Olive oil polyglycerol-6 esters, 2% Ethylhexyl stearate, 0.5% Dimethicone, 2% Isohexadecane, 5% Mineral oil, 0.15% Arginine, 0.5% p-Hydroxyacetophenone, 0.5% 1,2-Hexanediol, 0.7% Gentian extract, 0.3% Hydroxypropyl tetrahydropyrantriol, 0.05% Collagen, 7% Natural fermentation composition, 0.4% Fragrance, Balance: Water.

[0101] Comparative Example 5

[0102] A method for preparing a natural fermented oil composition includes the following steps: (1) Pretreatment of fermentation substrate: Mix rice bran oil, liquid avocado oil and wheat germ oil in a ratio of 30:2:6.5 and stir evenly to obtain mixed oil.

[0103] (2) Add 3kg of mixed oil to a 5L fermenter, turn on the stirrer at 180rpm, add 0.3kg of fermentation culture medium, sterilize at 116℃ for 20min, cool to 37℃, then add 0.3kg of Bacillus subtilis culture medium from Example 1, introduce sterile air for aerobic fermentation, fermentation time is 24h, and fermentation product 1 is obtained.

[0104] The fermentation broth comprises 15g peptone, 15g yeast powder, 15g beef extract, 1.5g NaCl, 1.5g dipotassium hydrogen phosphate, and 255g deionized water, with a pH of 6.5.

[0105] (3) When the temperature drops to 30°C, 0.3 kg of Lactobacillus plantarum culture solution from Example 1 is added at a mass ratio of 1:2. Anaerobic fermentation is carried out for 48 hours to obtain fermentation product 2.

[0106] (4) Take out fermentation product 2, lower the temperature to 4℃, put it into a high-speed homogenizer for high-pressure homogenization at a pressure of 30MPa, and cycle 3 times. Then centrifuge the fermentation product at 9000rpm for 20min, collect the oil phase, heat it to 55℃, adsorb it with 1wt% activated carbon in the oil phase for 1h, and then filter it through a 1.2μm fine filter plate and a 0.22μm filter screen to obtain the fermented composite oil.

[0107] (5) a. Aqueous phase: Mix 30 parts glycerol, 6 parts polyglycerol-10 myristate, 0.3 parts lecithin and 25.2 parts water at 80°C until homogeneous, and rotate at 30 rpm. b. Oil phase: 38.5 parts of fermented blend oil were stirred at 30 rpm at 80°C; c. Slowly add the oil phase to the water phase, homogenize at high speed (3000 rpm) for 6 minutes, stir for 20 minutes, cool down and filter with a 300 mesh to obtain the natural fermentation composition.

[0108] The high-speed homogenizer was purchased from Niro Soavi, Italy, model NS1001L Panda 2K.

[0109] (6) Prepare the scented body butter lotion according to the following proportions: 0.33% Acrylic (ester) compounds / C10-30 alkanol acrylate crosspolymer, 0.03% Disodium EDTA, 0.2% Allantoin, 3% Glycerin, 4% Glyceryl polyether-26, 0.04% Sodium hyaluronate, 0.03% Sodium polyglutamate, 1.5% Glyceryl mono- and glyceryl distearate, 0.5% Olive oil polyglycerol-6 esters, 2% Ethylhexyl stearate, 0.5% Dimethicone, 2% Isohexadecane, 5% Mineral oil, 0.15% Arginine, 0.5% p-Hydroxyacetophenone, 0.5% 1,2-Hexanediol, 0.7% Gentian extract, 0.3% Hydroxypropyl tetrahydropyrantriol, 0.05% Collagen, 7% Natural fermentation composition, 0.4% Fragrance, Balance: Water.

[0110] Comparative Example 6

[0111] A method for preparing a natural fermented oil composition includes the following steps: (1) Add 3kg of rice bran oil to a 5L fermenter, turn on the stirrer at 180rpm, add 0.3kg of fermentation culture medium, sterilize at 116℃ for 20min, cool to 37℃, then add 0.3kg of Bacillus subtilis culture medium from Example 1, introduce sterile air for aerobic fermentation, fermentation time is 24h, and obtain fermentation product 1.

[0112] The fermentation broth comprises 15g peptone, 15g yeast powder, 15g beef extract, 1.5g NaCl, 1.5g dipotassium hydrogen phosphate, and 255g deionized water, with a pH of 6.5.

[0113] (2) When the temperature drops to 30°C, 0.3 kg of the brewing yeast culture and Lactobacillus plantarum culture from Example 1 are added at a mass ratio of 1:2. Anaerobic fermentation is carried out for 48 hours to obtain fermentation product 2.

[0114] (3) Take out fermentation product 2, lower the temperature to 4℃, put it into a high-speed homogenizer for high-pressure homogenization at 30MPa, and cycle 3 times. Then centrifuge the fermentation product at 9000rpm for 20min, collect the oil phase, heat it to 55℃, adsorb it with 1wt% activated carbon in the oil phase for 1h, and then filter it through a 1.2μm fine filter plate and a 0.22μm filter screen to obtain fermented oil.

[0115] (4) a. Aqueous phase: Mix 30 parts glycerol, 6 parts polyglycerol-10 myristate, 0.3 parts lecithin and 25.2 parts water at 80°C until homogeneous, and rotate at 30 rpm. b. Oil phase: 38.5 parts of fermented oil were stirred at 30 rpm at 80°C; c. Slowly add the oil phase to the water phase, homogenize at high speed (3000 rpm) for 6 minutes, stir for 20 minutes, cool down and filter with a 300 mesh to obtain the natural fermentation composition.

[0116] The high-speed homogenizer was purchased from Niro Soavi, Italy, model NS1001L Panda 2K.

[0117] (5) Prepare the scented body butter lotion according to the following proportions: 0.33% Acrylic (ester) compounds / C10-30 alkanol acrylate crosspolymer, 0.03% Disodium EDTA, 0.2% Allantoin, 3% Glycerin, 4% Glyceryl polyether-26, 0.04% Sodium hyaluronate, 0.03% Sodium polyglutamate, 1.5% Glyceryl mono- and glyceryl distearate, 0.5% Olive oil polyglycerol-6 esters, 2% Ethylhexyl stearate, 0.5% Dimethicone, 2% Isohexadecane, 5% Mineral oil, 0.15% Arginine, 0.5% p-Hydroxyacetophenone, 0.5% 1,2-Hexanediol, 0.7% Gentian extract, 0.3% Hydroxypropyl tetrahydropyrantriol, 0.05% Collagen, 7% Natural fermentation composition, 0.4% Fragrance, Balance: Water.

[0118] Comparative Example 7

[0119] A method for preparing a natural fermented oil composition includes the following steps: (1) Add 3kg of liquid avocado fruit oil to a 5L fermenter, turn on the stirrer at 180rpm, add 0.3kg of fermentation culture medium, sterilize at 116℃ for 20min, cool to 37℃, then add 0.3kg of Bacillus subtilis culture medium from Example 1, introduce sterile air for aerobic fermentation, fermentation time is 24h, and obtain fermentation product 1.

[0120] The fermentation broth comprises 15g peptone, 15g yeast powder, 15g beef extract, 1.5g NaCl, 1.5g dipotassium hydrogen phosphate, and 255g deionized water, with a pH of 6.5.

[0121] (2) When the temperature drops to 30°C, 0.3 kg of the brewing yeast culture and Lactobacillus plantarum culture from Example 1 are added at a mass ratio of 1:2. Anaerobic fermentation is carried out for 48 hours to obtain fermentation product 2.

[0122] (3) Take out fermentation product 2, lower the temperature to 4℃, put it into a high-speed homogenizer for high-pressure homogenization at 30MPa, and cycle 3 times. Then centrifuge the fermentation product at 9000rpm for 20min, collect the oil phase, heat it to 55℃, adsorb it with 1wt% activated carbon in the oil phase for 1h, and then filter it through a 1.2μm fine filter plate and a 0.22μm filter screen to obtain fermented oil.

[0123] (4) a. Aqueous phase: Mix 30 parts glycerol, 6 parts polyglycerol-10 myristate, 0.3 parts lecithin and 25.2 parts water at 80°C until homogeneous, and rotate at 30 rpm. b. Oil phase: 38.5 parts of fermented oil were stirred at 30 rpm at 80°C; c. Slowly add the oil phase to the water phase, homogenize at high speed (3000 rpm) for 6 minutes, stir for 20 minutes, cool down and filter with a 300 mesh to obtain the natural fermentation composition.

[0124] The high-speed homogenizer was purchased from Niro Soavi, Italy, model NS1001L Panda 2K.

[0125] (6) Prepare the scented body butter lotion according to the following proportions: 0.33% Acrylic (ester) compounds / C10-30 alkanol acrylate crosspolymer, 0.03% Disodium EDTA, 0.2% Allantoin, 3% Glycerin, 4% Glyceryl polyether-26, 0.04% Sodium hyaluronate, 0.03% Sodium polyglutamate, 1.5% Glyceryl mono- and glyceryl distearate, 0.5% Olive oil polyglycerol-6 esters, 2% Ethylhexyl stearate, 0.5% Dimethicone, 2% Isohexadecane, 5% Mineral oil, 0.15% Arginine, 0.5% p-Hydroxyacetophenone, 0.5% 1,2-Hexanediol, 0.7% Gentian extract, 0.3% Hydroxypropyl tetrahydropyrantriol, 0.05% Collagen, 7% Natural fermentation composition, 0.4% Fragrance, Balance: Water.

[0126] Comparative Example 8

[0127] A method for preparing a natural fermented oil composition includes the following steps: (1) Add 3 kg of wheat germ oil to a 5L fermenter, turn on the stirrer at 180 rpm, add 0.3 kg of fermentation culture medium, sterilize at 116℃ for 20 min, cool to 37℃, then add 0.3 kg of Bacillus subtilis culture medium from Example 1, introduce sterile air for aerobic fermentation, fermentation time is 24 h, and obtain fermentation product 1.

[0128] The fermentation broth comprises 15g peptone, 15g yeast powder, 15g beef extract, 1.5g NaCl, 1.5g dipotassium hydrogen phosphate, and 255g deionized water, with a pH of 6.5.

[0129] (2) When the temperature drops to 30°C, 0.3 kg of the brewing yeast culture and Lactobacillus plantarum culture from Example 1 are added at a mass ratio of 1:2. Anaerobic fermentation is carried out for 48 hours to obtain fermentation product 2.

[0130] (3) Take out fermentation product 2, lower the temperature to 4℃, put it into a high-speed homogenizer for high-pressure homogenization at 30MPa, and cycle 3 times. Then centrifuge the fermentation product at 9000rpm for 20min, collect the oil phase, heat it to 55℃, adsorb it with 1wt% activated carbon in the oil phase for 1h, and then filter it through a 1.2μm fine filter plate and a 0.22μm filter screen to obtain fermented oil.

[0131] (4) a. Aqueous phase: Mix 30 parts glycerol, 6 parts polyglycerol-10 myristate, 0.3 parts lecithin and 25.2 parts water at 80°C until homogeneous, and rotate at 30 rpm. b. Oil phase: 38.5 parts of fermented oil were stirred at 30 rpm at 80°C; c. Slowly add the oil phase to the water phase, homogenize at high speed (3000 rpm) for 6 minutes, stir for 20 minutes, cool down and filter with a 300 mesh to obtain the natural fermentation composition.

[0132] The high-speed homogenizer was purchased from Niro Soavi, Italy, model NS1001L Panda 2K.

[0133] (5) Prepare the scented body butter lotion according to the following proportions: 0.33% Acrylic (ester) compounds / C10-30 alkanol acrylate crosspolymer, 0.03% Disodium EDTA, 0.2% Allantoin, 3% Glycerin, 4% Glyceryl polyether-26, 0.04% Sodium hyaluronate, 0.03% Sodium polyglutamate, 1.5% Glyceryl mono- and glyceryl distearate, 0.5% Olive oil polyglycerol-6 esters, 2% Ethylhexyl stearate, 0.5% Dimethicone, 2% Isohexadecane, 5% Mineral oil, 0.15% Arginine, 0.5% p-Hydroxyacetophenone, 0.5% 1,2-Hexanediol, 0.7% Gentian extract, 0.3% Hydroxypropyl tetrahydropyrantriol, 0.05% Collagen, 7% Natural fermentation composition, 0.4% Fragrance, Balance: Water.

[0134] Test case

[0135] Test Example 1: DPPH· Scavenging Rate Test Test method: The emulsions prepared in the examples and comparative examples were diluted with deionized water to a concentration of 1 mg / mL to serve as sample solutions. Vitamin C was used as a control group. 2.00 × 10⁻⁶ mg / mL of each emulsion was taken. -4 Add 3 mL each of mol / L DPPH·anhydrous ethanol solution and sample solution to a test tube, shake well, and let stand in the dark for 30 min. Then measure the absorbance Ai of the mixed solution at 517 nm. At the same time, measure the absorbance A0 of 3 mL of pure water mixed with 3 mL of DPPH·anhydrous ethanol solution and the absorbance Aj of 3 mL of sample solution mixed with 3 mL of anhydrous ethanol. Calculate the DPPH· scavenging rate (%) according to the formula.

[0136] DPPH· clearance rate (%) = [1 - (Ai - Aj) / A0] × 100% Test results: Table 1. Comparison of DPPH· clearance rate (%) test results

[0137] As can be seen from the table, the scented body butter lotion prepared in the embodiments of the present invention has good antioxidant activity.

[0138] Test Example 2: Human skin patch test of the scented body butter lotion of Example 2 Test subjects: 30 participants aged 18-60 years who were able to understand the test process, cooperated well with the test according to the requirements of the experimental protocol, maintained a good lifestyle, voluntarily participated in the test, and signed a written informed consent form.

[0139] Test method: Select an area not exceeding 50mm² 2 A qualified spot test apparatus with a depth of approximately 1 mm is used. The test substance is placed in the small chamber of the spot test apparatus, with a dosage of approximately 0.020 g to 0.025 g. The test substance is the emulsion from Example 2, and the control well is a blank control (without any substance). The spot test apparatus containing the test substance is applied to the back or flexor side of the forearm of the subject using hypoallergenic adhesive tape, and gently pressed with the palm of the hand to ensure even application to the skin, for 24 hours. Thirty minutes after removing the spot test apparatus, the skin reaction is observed according to the standards in Table 1, and the results are recorded. Recordings are repeated at 24 hours and 48 hours.

[0140] Table 2. Grading Criteria for Skin Reaction in Closed Patch Tests

[0141] Table 3. Results of skin reaction test in closed patch test of subjects

[0142] Test results: Under normal conditions, all 30 subjects showed negative reactions to the test sample, as did all 30 subjects in the blank control group. This indicates that the emulsion made from the natural fermentation composition has a mild and non-irritating effect.

[0143] Test Example 3: Repair Efficacy Test Test subjects: 110 participants aged 18 to 30 years old, randomly divided into 11 groups of 10 people each. They were healthy men and women with sensitive facial skin and an average hemoglobin level greater than 0.900 within a 24mm diameter circle on their face. They were able to understand the test process, cooperate well with the test according to the experimental protocol, maintain a good lifestyle, voluntarily participate in the test, and sign a written informed consent form.

[0144] Test Method: Subjects cleansed their faces with water and dried them with a lint-free cloth or dry facial tissue. They sat quietly for 30 minutes in a constant temperature and humidity chamber at 21±1℃ and 50±10% relative humidity. The test areas (face) were photographed using the ANTERA 3D multifunctional 3D skin imaging analyzer. The transepidermal water loss was measured in each test area using a VapoMeter transepidermal water loss meter, with three parallel measurements per area. The MoistureMeterSC stratum corneum moisture content was then measured in each test area, with three parallel measurements per area. Data were collected at weeks 0 and 4 for samples from the example and comparative studies. The rate of increase in moisture content (in AU) and the rate of decrease in transepidermal water loss (in g / m²) were calculated. 2 h), the rate of decrease in average blood color level.

[0145] Table 4 Test Results

[0146] As can be seen from Table 4, after 4 weeks of using the test sample, there were significant improvements in the moisture content of the stratum corneum, the loss of epidermal moisture, and the average level of facial hemoglobin, indicating that it has excellent repair effects.

[0147] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention and not to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A method for preparing a naturally fermented oil composition, characterized in that, Includes the following steps: (1) Mix rice bran oil, liquid avocado oil, and wheat germ oil evenly to obtain a mixed oil; (2) Add the fermentation culture medium to the mixed oil, mix evenly, sterilize, inoculate with Bacillus subtilis culture medium for aerobic fermentation, then add the inoculum culture medium for anaerobic fermentation, homogenize, centrifuge, collect the oil phase, add activated carbon for adsorption, filter, and obtain fermented combined oil; the inoculum culture medium includes brewer's yeast culture medium and Lactobacillus plantarum culture medium in a mass ratio of (1~2):(2~3); (3) Mix glycerol, polyglycerol-10 myristate, lecithin and water evenly, then mix with fermented combination oil evenly and homogenize to obtain natural fermented oil composition.

2. The method for preparing the natural fermented oil composition according to claim 1, characterized in that, The mass ratio of rice bran oil, liquid avocado oil, and wheat germ oil is (25~30):(1~2):(6~7).

3. The method for preparing the natural fermented oil composition according to claim 1, characterized in that, The fermentation broth comprises the following components by mass percentage: 1%~5% peptone, 1%~5% yeast extract, 1%~5% beef extract, 0.1%~0.5% NaCl, 0.1%~0.5% dipotassium hydrogen phosphate, and the balance being deionized water.

4. The method for preparing the natural fermented oil composition according to claim 1, characterized in that, The mass ratio of the fermentation broth to the mixed oil is (0.05~0.1):1; The mass ratio of the mixed oil, Bacillus subtilis culture medium, and bacterial culture medium is 1:(0.05~0.1):(0.05~0.1).

5. The method for preparing the natural fermented oil composition according to claim 1, characterized in that, The concentration of Bacillus subtilis in the culture medium was 10. 8 ~10 9 cfu / mL; The concentration of Saccharomyces cerevisiae in the culture medium is 10. 8 ~10 9 cfu / mL; The concentration of *Lactobacillus plantarum* in the culture medium is 10. 8 ~10 9 cfu / mL.

6. The method for preparing the natural fermented oil composition according to claim 1, characterized in that, The aerobic fermentation temperature is 35~37℃, and the fermentation time is 18~30h; The anaerobic fermentation temperature is 28~30℃, and the fermentation time is 40~56h.

7. The method for preparing the natural fermented oil composition according to claim 1, characterized in that, The mass ratio of glycerol, polyglycerol-10 myristate, lecithin, water, and fermented oil is (30~35):(5~6):(0.2~0.3):(25~30):(38~40).

8. A naturally fermented oil composition, characterized in that, It is prepared by any of the preparation methods described in claims 1 to 7.

9. The use of the natural fermented oil composition according to claim 8 in the preparation of cosmetics.

10. A scented body butter lotion, characterized in that, It comprises the following components by weight percentage: 0.2%–0.6% thickener, 0.01%–0.15% chelating agent, 0.1%–3% skin conditioning agent, 1%–3% emulsifier, 5%–15% emollient, 5%–15% moisturizer, 0.1%–0.3% pH adjuster, 0.5%–10% naturally fermented oil composition, 0.01%–0.5% fragrance, 0.3%–1.5% preservative, and the balance being solvent; The natural fermentation composition is the natural fermentation oil composition according to claim 8.

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