Anti-blue-light anti-aging multifunctional microsphere composition, preparation method and application thereof, and skin care product

Abstract

The invention discloses an anti-blue light and anti-aging multifunctional microsphere composition, a preparation method and application thereof and a skin care product, the microsphere composition comprises anti-blue light and anti-aging microspheres and dipotassium glycyrrhizinate, and the mass ratio of the anti-blue light and anti-aging microspheres to the dipotassium glycyrrhizinate is (0.1-8): (0.01-3). According to the anti-blue-light anti-aging multifunctional microsphere composition, the preparation method and application thereof and the skin care product, the hyaluronic acid microspheres are adopted as a core carrier, the three-dimensional network structure of the hyaluronic acid microspheres constructs a stable protection environment, the industrial problem that the stability of active ingredients is poor is effectively solved, and the stability of the active ingredients can be effectively improved.

Description

Technical Field

[0001] This invention belongs to the field of skin care ingredient technology, and relates to a microsphere composition, particularly to a multifunctional microsphere composition for anti-blue light and anti-aging, its preparation method, application, and skin care products. Background Technology

[0002] In modern society, with the widespread use of electronic devices such as smartphones, tablets, and computer monitors, people are exposed to blue light for significantly longer periods. Blue light mainly originates from the screens of these electronic devices and artificial light sources such as LED lights and fluorescent lamps. Its wavelength is concentrated around 400-450 nanometers and has high energy. Blue light can penetrate the epidermis to the dermis. Its energy characteristics induce the skin to produce free radicals and activate oxidative stress, leading to lipid peroxidation and DNA damage. Blue light stimulates fibroblasts to secrete excessive amounts of matrix metalloproteinases (MMP-1 / MMP-3 / MMP-9), causing irreversible degradation of collagen and elastin fibers in the dermis, accelerating skin laxity and wrinkle formation. The disordered arrangement of lipids in the stratum corneum leads to a decline in barrier function, further exacerbating moisture loss and sensitivity to external stimuli. Under the cumulative effect of long-term exposure, the skin exhibits loss of elasticity, pathological pigmentation, and photoaging phenotypes.

[0003] Current blue light blocking and anti-aging products on the market have many shortcomings. Most blue light blocking products only focus on physically blocking or partially absorbing blue light, adding inorganic particles such as titanium dioxide or zinc oxide to reflect blue light. However, these ingredients mainly act on the skin surface and cannot penetrate deep into the skin to remove free radicals induced by blue light, making it difficult to substantially improve the skin's internal repair and antioxidant capacity. Common anti-aging products typically contain only a single active ingredient, lacking a multi-pathway synergistic mechanism targeting matrix degradation and barrier repair, making it difficult to address the combined effects of blue light-induced oxidative damage, collagen loss, and barrier disruption. Furthermore, many active ingredients are inherently unstable and easily deactivated by factors such as light, temperature, and oxygen, leading to reduced product efficacy. Regarding release control, most active ingredients in existing products are directly added to the cosmetic matrix, making precise release control difficult. This hinders effective penetration into specific skin layers and lacks targeting, easily resulting in resource waste and adverse skin reactions.

[0004] Chinese invention patent CN110236965A discloses a method of directly adding active ingredients (such as carotene and saffron extract) to a matrix. This method lacks a protective mechanism and is easily deactivated by light, oxygen, and temperature, resulting in insufficient long-term stability. Furthermore, its action is limited to the epidermis and cannot penetrate the dermis to eliminate blue light-induced free radicals or block collagen degradation. This technology relies on single-dimensional physical absorption of blue light and surface antioxidation, without integrating multi-pathway synergistic mechanisms such as anti-inflammation, barrier repair, and matrix metalloproteinase (MMP) inhibition. Therefore, it struggles to address the combined effects of blue light-induced oxidative damage, inflammatory responses, and collagen loss. The release of ingredients is uncontrollable, lacking sustained-release systems and targeted delivery technologies. Active ingredients are rapidly released or remain on the epidermis, failing to achieve deep and precise repair.

[0005] In view of this, there is an urgent need to design a new anti-blue light and anti-aging skin care product in order to overcome at least some of the aforementioned shortcomings of existing skin care products. Summary of the Invention

[0006] This invention provides a multifunctional microsphere composition for anti-blue light and anti-aging, its preparation method, application, and skin care products, which can improve the stability of active ingredients.

[0007] To solve the above-mentioned technical problems, according to one aspect of the present invention, the following technical solution is adopted:

[0008] A multifunctional microsphere composition for anti-blue light and anti-aging, the microsphere composition comprising: anti-blue light and anti-aging microspheres and dipotassium glycyrrhizate, wherein the mass ratio of the anti-blue light and anti-aging microspheres to dipotassium glycyrrhizate is (0.1-8):(0.01-3).

[0009] In one embodiment of the present invention, the mass ratio of the anti-blue light and anti-aging microspheres to dipotassium glycyrrhizate is (0.1-5):(0.01-1).

[0010] In one embodiment of the present invention, the anti-blue light anti-aging microspheres include superoxide dismutase and saffron extract; the mass ratio of superoxide dismutase to saffron extract in the anti-blue light anti-aging microspheres is (5-15):(5-12).

[0011] As one embodiment of the present invention, the preparation method of the anti-blue light and anti-aging microspheres includes:

[0012] Step S1: Constructing a core layer activity protection system;

[0013] Raw material mixing steps: Dissolve 1.0-2.0 parts by weight of superoxide dismutase (SOD), 5.0-6.0 parts by weight of sodium alginate, and 0.3-0.5 parts by weight of maltodextrin in pre-cooled biocompatible buffer A with a pH of 6-7, and stir to form a homogeneous core phase solution;

[0014] Ion crosslinking molding step: The core phase solution is injected into a water-soluble calcium salt solution, stirred, and solidified to generate a porous gel core; free calcium ions are removed by washing with deionized water to obtain core layer gel particles with uniform particle size.

[0015] Step S2: Functionalization of blue light absorption in the shell layer;

[0016] Preparation steps of self-assembled complex: Dissolve 3.0-4.0 parts by weight of hyaluronic acid in a weakly acidic buffer solution B with a pH of 4-5. After dissolving by stirring in a water bath, add 1.0-1.5 parts by weight of saffron extract and 0.1-0.2 parts by weight of tea polyphenols. Sonicate in the dark and let stand for a set time to form an aqueous shell solution.

[0017] Interfacial polymerization coating steps: The core layer gel particles are dispersed in a hydrophobic ester solvent, and 0.1-0.15 parts by weight of PEG-40 hydrogenated castor oil is added as an emulsifier. Shear emulsification is performed to form a W / O primary emulsion. The shell layer aqueous solution is slowly added to the primary emulsion, and shear emulsification is continued to form a W / O / W secondary emulsion. The emulsion is then transferred to a water bath, and the shell coating is completed through reaction to form microspheres.

[0018] Step S3: Crosslinking curing and surface modification steps;

[0019] Bio-crosslinking enhancement step: Immerse the microspheres in 0.05-0.2% w / v biocompatible cationic polymer solution C, and slowly shake to enhance shell stability through electrostatic interaction, followed by washing with buffer solution with pH 7.1-8;

[0020] Visible light responsive curing step: The microspheres are photocured using a light source and stirred simultaneously to ensure uniform cross-linking.

[0021] According to another aspect of the present invention, the following technical solution is adopted: a skin care product comprising the above-mentioned anti-blue light and anti-aging multifunctional microsphere composition.

[0022] According to another aspect of the present invention, the following technical solution is adopted: a method for preparing anti-blue light and anti-aging microspheres, the preparation method comprising the following steps:

[0023] Step S1: Constructing a core layer activity protection system;

[0024] Raw material mixing steps: Dissolve 1.0-2.0 parts by weight of superoxide dismutase (SOD), 5.0-6.0 parts by weight of sodium alginate, and 0.3-0.5 parts by weight of maltodextrin in pre-cooled phosphate buffer solution with a pH of 6-7, and stir to form a homogeneous core phase solution;

[0025] Ion crosslinking molding step: The core phase solution is injected into a calcium lactate crosslinking bath, stirred, and solidified to generate a porous gel core; free calcium ions are removed by washing with deionized water to obtain core layer gel particles with uniform particle size.

[0026] Step S2: Functionalization of blue light absorption in the shell layer;

[0027] Preparation steps of self-assembled complex: Dissolve 3.0-4.0 parts by weight of hyaluronic acid in citrate buffer solution with a pH of 4-5, stir and dissolve in a water bath, add 1.0-1.5 parts by weight of saffron extract and 0.1-0.2 parts by weight of tea polyphenols, treat with ultrasound in the dark, and let stand for a set time to form a shell aqueous solution.

[0028] Interfacial polymerization coating steps: Core gel particles are dispersed in glyceryl caprylate and capric acid, and 0.1–0.15 parts by weight of PEG-40 hydrogenated castor oil is added as an emulsifier. Shear emulsification is then performed to form a W / O emulsion. The shell aqueous solution is slowly added to the primary emulsion, and shear emulsification continues to form a W / O / W complex emulsion. This complex emulsion is then transferred to a water bath, and the shell coating is completed through reaction, forming microspheres.

[0029] Step S3: Crosslinking curing and surface modification steps;

[0030] Bio-crosslinking enhancement step: Immerse the microspheres in a 0.05-0.2% w / v polylysine solution and shake slowly to enhance shell stability through electrostatic interaction, then wash with a buffer solution with a pH of 7.1-8;

[0031] Visible light responsive curing step: The microspheres are photocured using a light source and stirred simultaneously to ensure uniform cross-linking.

[0032] In one embodiment of the present invention, the biocompatible buffer A includes at least one of phosphate buffer, HEPES buffer, and MES buffer;

[0033] The water-soluble calcium salt solution is a water-soluble calcium salt solution, which includes at least one of calcium lactate, calcium chloride, and calcium gluconate;

[0034] The weakly acidic buffer B includes at least one of citrate buffer and acetate-sodium acetate buffer;

[0035] The hydrophobic ester solvent includes at least one of glyceryl caprylate or isononyl isononanoate;

[0036] The biocompatible cationic polymer solution C includes at least one of polylysine and ε-polylysine solution.

[0037] The biocompatible buffer A is a phosphate buffer, the water-soluble calcium salt solution is calcium lactate, the buffer B is a citrate buffer, the hydrophobic ester solvent is caprylic / capric glycerol, and the biocompatible cationic polymer solution C is polylysine.

[0038] According to another aspect of the present invention, the following technical solution is adopted: a method for preparing an anti-blue light anti-aging microsphere composition, the preparation method comprising: preparing anti-blue light anti-aging microspheres, mixing anti-blue light anti-aging microspheres and dipotassium glycyrrhizate in a set mass ratio to obtain an anti-blue light anti-aging microsphere composition; wherein the mass ratio of the anti-blue light anti-aging microspheres to dipotassium glycyrrhizate is (0.1-8):(0.01-3);

[0039] According to another aspect of the present invention, the following technical solution is adopted: the application of the above-mentioned anti-blue light and anti-aging multifunctional microsphere composition in the preparation of skin care products.

[0040] In one embodiment of the present invention, the skin care product is one of skin care water, serum, lotion, face cream, or freeze-dried powder.

[0041] The beneficial effects of this invention are as follows: The multifunctional microsphere composition for anti-blue light and anti-aging, its preparation method, application, and skincare products proposed in this invention utilize hyaluronic acid microspheres as the core carrier. Their three-dimensional network structure creates a stable protective environment, effectively solving the industry problem of poor stability of active ingredients and significantly improving their stability. On one hand, by physically encapsulating and immobilizing superoxide dismutase (SOD) through an ion-crosslinking network, its half-life is significantly extended, ensuring the long-term effectiveness of free radical scavenging. On the other hand, utilizing the sustained-release properties of the microspheres, SOD and saffron extract are precisely controlled for release in the skin, penetrating deep to exert their antioxidant effects. Dipotassium glycyrrhizate, through microsphere targeted delivery technology, precisely targets areas of active inflammation, inhibiting tyrosinase activity and chelating metal ions to block the melanin synthesis signaling pathway at its source, achieving targeted repair.

[0042] This invention constructs a unique core-shell protection system, forming a dual blue light barrier of "oxidation neutralization-light absorption." The core layer uses ion cross-linking technology to fix SOD, constructing a frontline defense against oxidative stress and targeting and eliminating excess free radicals induced by blue light. The shell layer utilizes the conjugated structural properties of crocin to form a specific light absorption barrier, converting high-energy blue light energy into harmless heat energy and reducing energy transmission to the dermis. This core-shell structure overcomes the limitations of single-dimensional protection, achieving synergistic effects of photoprotection and antioxidation. Experimental data show that this system increases the inhibition rate of intracellular ROS generation, significantly enhances the inhibition rate of MMP-1 / MMP-3 synthesis, and effectively delays collagen degradation. Attached Figure Description

[0043] Figure 1This is a flowchart of a method for preparing anti-blue light and anti-aging microspheres in one embodiment of the present invention.

[0044] Figure 2 This is a skin texture diagram before using the serum from Example 3.

[0045] Figure 3 Skin texture image after two weeks of using the serum from Example 3.

[0046] Figure 4 Skin texture diagram after four weeks of using the serum from Example 3.

[0047] Figure 5 Image showing under-eye wrinkles before using the serum in Example 3.

[0048] Figure 6 The image shows under-eye wrinkles two weeks after using the serum from Example 3.

[0049] Figure 7 The image shows under-eye wrinkles four weeks after using the serum from Example 3. Detailed Implementation

[0050] The preferred embodiments of the present invention will now be described in detail with reference to the accompanying drawings.

[0051] To further understand the present invention, preferred embodiments of the present invention are described below in conjunction with examples. However, it should be understood that these descriptions are only for further illustrating the features and advantages of the present invention, and not for limiting the scope of the claims of the present invention.

[0052] The description in this section pertains to only a few typical embodiments, and the present invention is not limited to the scope of the embodiments described. Substitution of identical or similar prior art methods with some technical features in the embodiments is also within the scope of the description and protection of this invention.

[0053] Unless otherwise stated, implied from the context, or as is customary in the art, all parts and percentages in this application are based on weight, and all testing and characterization methods used are concurrent with the filing date of this application. Where applicable, any patent, patent application, or disclosure relating to this application is incorporated herein by reference in its entirety, and its equivalent patent families are also incorporated herein by reference, particularly the definitions disclosed in these documents concerning synthetic techniques, product and processing design, polymers, comonomers, initiators, or catalysts in the art. If any definition of a specific term disclosed in the prior art is inconsistent with any definition provided in this application, the definition provided in this application shall prevail.

[0054] The numerical ranges in this application are approximate values ​​and therefore may include values ​​outside the range unless otherwise stated. A numerical range includes all values ​​from the lower limit to the upper limit, increasing by one unit, provided there is an interval of at least two units between any lower and any higher value. For example, if a component, physical, or other property (such as molecular weight, melt index, etc.) is described as 100 to 1000, this means that all individual values, such as 100, 101, 102, etc., are explicitly listed, as well as all subranges, such as 100 to 166, 155 to 170, 198 to 200, etc. For ranges containing values ​​less than 1 or fractions greater than 1 (e.g., 1.1, 1.5, etc.), one unit is appropriately considered as 0.0001, 0.001, 0.01, or 0.1. For ranges containing single digits less than 10 (e.g., 1 to 5), one unit is generally considered as 0.1. These are merely specific examples of what is intended to be expressed, and all possible combinations of values ​​between the listed minimum and maximum values ​​are considered to be clearly stated in this application. It should also be noted that the terms "first," "second," etc., used herein are not intended to specify a particular order, but are merely used to distinguish substances with different structures.

[0055] When referring to chemical compounds, unless explicitly stated otherwise, the singular includes all isomers and vice versa (e.g., "hexane" includes all isomers of hexane, individually or collectively). Additionally, unless explicitly stated otherwise, nouns described with "an," "a," or "the" also include their plural forms.

[0056] The terms “comprising,” “including,” “having,” and their derivatives do not exclude the presence of any other components, steps, or processes, regardless of whether such other components, steps, or processes are disclosed in this application. To eliminate any doubt, unless expressly stated otherwise, all compositions using the terms “comprising,” “including,” or “having” in this application may contain any additional additives, excipients, or compounds. Conversely, except for those necessary for operational performance, the term “substantially constitutes…” excludes any other components, steps, or processes described below with respect to that term. The term “consisting of…” does not include any components, steps, or processes not specifically described or listed. Unless expressly stated otherwise, the term “or” refers to the individual members listed or any combination thereof.

[0057] The steps described in the various embodiments in the specification are for illustrative purposes only, and the implementation of this application is not limited by the order of the steps.

[0058] This invention discloses a multifunctional microsphere composition for anti-blue light and anti-aging, the microsphere composition comprising: anti-blue light and anti-aging microspheres and dipotassium glycyrrhizate, wherein the mass ratio of the anti-blue light and anti-aging microspheres to dipotassium glycyrrhizate is (0.1-8):(0.01-3).

[0059] In one embodiment of the present invention, the mass ratio of the anti-blue light anti-aging microspheres to dipotassium glycyrrhizate is (0.1-5):(0.01-1). In another embodiment, the anti-blue light anti-aging microspheres include superoxide dismutase and saffron extract, and the mass ratio of superoxide dismutase to saffron extract in the anti-blue light anti-aging microspheres is (5-15):(5-12).

[0060] Superoxide dismutase (SOD), as a core component of the endogenous antioxidant system, efficiently catalyzes superoxide anion free radicals (O2). - Superoxide (SOD) is converted into hydrogen peroxide and oxygen, directly blocking the free radical chain reaction and becoming the core active substance in combating blue light damage. Its ability to target and clear blue light-induced mitochondrial ROS significantly reduces the level of DNA oxidation markers, while simultaneously inhibiting the expression of matrix metalloproteinases (MMP-1 / MMP-3), protecting the dermal collagen and elastic fiber network, and thus delaying photoaging from its source. SOD regulates the NF-κB / Nrf2 dual pathway, inhibiting the release of inflammatory factors (TNF-α, IL-6) while activating the expression of endogenous antioxidant enzymes, synergistically repairing the lipid barrier and reducing transepidermal water loss (TEWL). Furthermore, SOD effectively reduces the area of ​​age spots by blocking the upregulation of the ROS-induced α-MSH / MC1R signaling pathway, inhibiting the activation of tyrosinase by the bypass pathway, and reducing lipofuscin deposition.

[0061] Saffron extract is rich in antioxidants such as crocin and crocinic acid. Its conjugated double bond structure can specifically absorb the blue light band, reducing blue light transmission through physical blocking. Simultaneously, it quenches free radicals and neutralizes reactive oxygen species (ROS) caused by photodamage through electron transfer, forming a dual "absorption-scavenging" protection mechanism. This component reduces the release of inflammatory factors by inhibiting the NF-κB signaling pathway and activates the Nrf2 pathway to promote the expression of endogenous antioxidant enzymes, synergistically repairing the stratum corneum lipid barrier and tight junction proteins, significantly improving barrier integrity. Furthermore, crocinic acid competitively binds to metal ions at the active site of tyrosinase, inhibiting enzyme activity and melanin synthesis, while interfering with the function of melanosome transport proteins, reducing pigmentation and resulting in a more even and fairer skin tone. Targeting collagen loss due to photoaging, saffron extract can downregulate the expression of matrix metalloproteinases (MMPs), protecting the dermal collagen and elastic fiber network.

[0062] Dipotassium glycyrrhizate effectively inhibits the release of pro-inflammatory factors and stabilizes mast cell membranes by targeting and regulating the TLR4 / MyD88-NF-κB inflammatory pathway, thereby rapidly alleviating neurogenic sensitivity symptoms such as skin burning and erythema caused by blue light radiation. At the barrier repair level, it significantly enhances the stratum corneum's hydration capacity and reduces transepidermal water loss by activating PPARγ receptors to promote ceramide synthesis and tight junction protein expression, achieving dual repair from inflammation control to physical barrier reconstruction. Dipotassium glycyrrhizate directly inhibits melanin production by chelating metal ions at the active site of tyrosinase and interferes with Rab27a-mediated melanosome transport, simultaneously blocking the synthesis and transfer pathways of pigment deposition. Regarding blue light-induced oxidative damage, its phenolic hydroxyl structure can quench free radicals, enhance mitochondrial antioxidant defense by activating the SIRT1 / PGC-1α pathway, and simultaneously inhibit the ROS-TRP signaling cascade, delaying photoaging and thus providing comprehensive anti-aging protection for the skin.

[0063] Figure 1 This is a flowchart of a method for preparing anti-blue light and anti-aging microspheres according to an embodiment of the present invention; please refer to [link / reference]. Figure 1 In one embodiment of the present invention, the preparation method of the anti-blue light and anti-aging microspheres includes:

[0064] Step S1: Constructing a core layer activity protection system;

[0065] Raw material mixing steps: Dissolve 1.0-2.0 parts by weight of superoxide dismutase (SOD), 5.0-6.0 parts by weight of sodium alginate, and 0.3-0.5 parts by weight of maltodextrin in pre-cooled biocompatible buffer A with a pH of 6-7, and stir to form a homogeneous core phase solution;

[0066] Ion crosslinking molding step: The core phase solution is injected into a water-soluble calcium salt solution, stirred, and solidified to generate a porous gel core; free calcium ions are removed by washing with deionized water to obtain core layer gel particles with uniform particle size.

[0067] Step S2: Functionalization of blue light absorption in the shell layer;

[0068] Preparation steps of self-assembled complex: Dissolve 3.0-4.0 parts by weight of hyaluronic acid in a weakly acidic buffer solution B with a pH of 4-5. After dissolving by stirring in a water bath, add 1.0-1.5 parts by weight of saffron extract and 0.1-0.2 parts by weight of tea polyphenols. Sonicate in the dark and let stand for a set time to form an aqueous shell solution.

[0069] Interfacial polymerization coating steps: The core layer gel particles are dispersed in a hydrophobic ester solvent, and 0.1-0.15 parts by weight of PEG-40 hydrogenated castor oil is added as an emulsifier. Shear emulsification is performed to form a W / O primary emulsion. The shell layer aqueous solution is slowly added to the primary emulsion, and shear emulsification is continued to form a W / O / W secondary emulsion. The emulsion is then transferred to a water bath, and the shell coating is completed through reaction to form microspheres.

[0070] Step S3: Crosslinking curing and surface modification steps;

[0071] Bio-crosslinking enhancement step: Immerse the microspheres in 0.05-0.2% w / v biocompatible cationic polymer solution C, and slowly shake to enhance shell stability through electrostatic interaction, followed by washing with buffer solution with pH 7.1-8;

[0072] Visible light responsive curing step: The microspheres are photocured using a light source and stirred simultaneously to ensure uniform cross-linking.

[0073] The biocompatibility buffer A includes at least one of phosphate buffer, HEPES buffer, and MES buffer.

[0074] The water-soluble calcium salt solution is a water-soluble calcium salt solution, which includes at least one of calcium lactate, calcium chloride, and calcium gluconate;

[0075] The weakly acidic buffer B includes at least one of citrate buffer and acetate-sodium acetate buffer;

[0076] The hydrophobic ester solvent includes at least one of glyceryl caprylate or isononyl isononanoate;

[0077] The biocompatible cationic polymer solution C includes at least one of polylysine and ε-polylysine solution.

[0078] In one embodiment of the present invention, the biocompatible buffer A is a phosphate buffer, the water-soluble calcium salt solution is calcium lactate, the buffer B is a citrate buffer, the hydrophobic ester solvent is caprylic / capric glycerol, and the biocompatible cationic polymer solution C is polylysine.

[0079] The present invention also discloses a skin care product comprising the above-mentioned anti-blue light and anti-aging multifunctional microsphere composition.

[0080] This invention further discloses a method for preparing anti-blue light and anti-aging microspheres, the method comprising the following steps:

[0081] Step S1: Constructing a core layer activity protection system;

[0082] Raw material mixing steps: Dissolve 1.0-2.0 parts by weight of superoxide dismutase (SOD), 5.0-6.0 parts by weight of sodium alginate, and 0.3-0.5 parts by weight of maltodextrin in pre-cooled phosphate buffer solution with a pH of 6-7, and stir to form a homogeneous core phase solution;

[0083] Ion crosslinking molding step: The core phase solution is injected into a calcium lactate crosslinking bath, stirred, and solidified to generate a porous gel core; free calcium ions are removed by washing with deionized water to obtain core layer gel particles with uniform particle size.

[0084] Step S2: Functionalization of blue light absorption in the shell layer;

[0085] Preparation steps of self-assembled complex: Dissolve 3.0-4.0 parts by weight of hyaluronic acid in citrate buffer solution with a pH of 4-5, stir and dissolve in a water bath, add 1.0-1.5 parts by weight of saffron extract and 0.1-0.2 parts by weight of tea polyphenols, treat with ultrasound in the dark, and let stand for a set time to form a shell aqueous solution.

[0086] Interfacial polymerization coating steps: Core gel particles are dispersed in glyceryl caprylate and capric acid, and 0.1–0.15 parts by weight of PEG-40 hydrogenated castor oil is added as an emulsifier. Shear emulsification is then performed to form a W / O emulsion. The shell aqueous solution is slowly added to the primary emulsion, and shear emulsification continues to form a W / O / W complex emulsion. This complex emulsion is then transferred to a water bath, and the shell coating is completed through reaction, forming microspheres.

[0087] Step S3: Crosslinking curing and surface modification steps;

[0088] Bio-crosslinking enhancement step: Immerse the microspheres in a 0.05-0.2% w / v polylysine solution and shake slowly to enhance shell stability through electrostatic interaction, then wash with a buffer solution with a pH of 7.1-8;

[0089] Visible light responsive curing step: The microspheres are photocured using a light source and stirred simultaneously to ensure uniform cross-linking.

[0090] In one embodiment of the present invention, the preparation method of the anti-blue light and anti-aging microspheres includes the following steps:

[0091] Step S1: Constructing a core layer activity protection system;

[0092] Raw material mixing: Dissolve 1.0-2.0 g (e.g., 1.0-2.0 g) of superoxide dismutase (SOD), 5.0-6.0 g (e.g., 5.0-6.0 g) of sodium alginate, and 0.3-0.5 g (e.g., 0.3-0.5 g) of maltodextrin in a pre-cooled phosphate buffer solution with a pH of 6-7 (e.g., pH 6.8) (e.g., 500 mL). Stir magnetically (at 500 rpm) for 30 minutes to form a homogeneous solution. Continue stirring at 4°C in the dark for 2 hours to stabilize the SOD conformation, finally forming a homogeneous core phase solution.

[0093] Ion crosslinking molding: The core phase solution was injected into a 1.2 mol / L calcium lactate solution through a microfluidic nozzle (150 μm inner diameter), and the core phase flow rate was controlled at 1 mL / min and the aqueous phase flow rate at 15 mL / min. The solution was continuously stirred at 25°C (200 rpm) for 45 minutes to solidify and generate a porous gel core. Free calcium ions were removed by washing with deionized water three times to obtain core layer gel particles with uniform particle size.

[0094] Step S2: Functionalization of blue light absorption in the shell layer;

[0095] Preparation of self-assembled complex: Dissolve 3.0-4.0 g (e.g., 3.0-4.0 g) of hyaluronic acid in an appropriate amount of citrate buffer (e.g., 300 mL) with a pH of 4-5 (pH can be 4.5). After dissolving by stirring in a water bath at 40°C, add 1.0-1.5 g (e.g., 1.0-1.5 g) of saffron extract and 0.1-0.2 g (e.g., 0.1-0.2 g) of tea polyphenols. Sonicate in the dark (40 kHz, 100 W) for 15 minutes and let stand for a set time (e.g., 2 hours) to form a shell aqueous solution.

[0096] Interfacial polymerization coating: Core gel particles were dispersed in caprylic / capric triglyceride, and 0.1-0.15 parts by weight of PEG-40 hydrogenated castor oil were added as an emulsifier. The mixture was sheared at 8000 rpm for 10 minutes to form a W / O emulsion. The shell aqueous solution was slowly added to the primary emulsion, and shearing emulsification was continued for 10 minutes to form a W / O / W complex emulsion. The mixture was then transferred to a 40°C constant temperature water bath and reacted for 5 hours to complete the shell coating and form microspheres.

[0097] Step S3: Crosslinking curing and surface modification steps;

[0098] Bio-crosslinking enhancement: The microspheres were immersed in a polylysine solution of 0.1% w / v (other values ​​are also possible, such as 0.05% w / v, 0.08% w / v, 0.15% w / v, 0.2% w / v, etc.) and slowly shaken (50 rpm) at 4°C for 18 hours to enhance shell stability through electrostatic interaction. The microspheres were then washed three times with PBS buffer at pH 7.1–8 (pH 7.4 is acceptable).

[0099] Visible light responsive curing: using a 405nm LED light source (intensity 40mW / cm²). 2 The microspheres were photocured for 15 minutes while being stirred simultaneously (100 rpm) to ensure uniform cross-linking.

[0100] This invention further discloses a method for preparing an anti-blue light anti-aging microsphere composition, the method comprising: preparing anti-blue light anti-aging microspheres by mixing anti-blue light anti-aging microspheres and dipotassium glycyrrhizate in a set mass ratio to obtain an anti-blue light anti-aging microsphere composition; wherein the mass ratio of the anti-blue light anti-aging microspheres to dipotassium glycyrrhizate is (0.1-8):(0.01-3);

[0101] This invention further discloses the application of the above-mentioned anti-blue light and anti-aging multifunctional microsphere composition in the preparation of skin care products. The skin care products can be one of the following: skin care water, serum, lotion, cream, or freeze-dried powder.

[0102] Example 1

[0103] The composition of the present invention consists of the following components in parts by weight: 5 parts of anti-blue light and anti-aging microspheres and 1 part of dipotassium glycyrrhizate.

[0104] Example 2

[0105] The composition of the present invention consists of the following components in parts by weight: 8 parts of anti-blue light and anti-aging microspheres and 3 parts of dipotassium glycyrrhizate.

[0106] Example 3

[0107] The composition of the present invention consists of the following components in parts by weight: 2 parts of anti-blue light and anti-aging microspheres and 0.5 parts of dipotassium glycyrrhizate.

[0108] Comparative Example 1

[0109] The composition of the present invention consists of the following components in parts by weight: 2.5 parts of anti-blue light and anti-aging microspheres and 0 parts of dipotassium glycyrrhizate.

[0110] Comparative Example 2

[0111] The composition of the present invention consists of the following components in parts by weight: 0 parts of anti-blue light and anti-aging microspheres and 2.5 parts of dipotassium glycyrrhizate.

[0112] Comparative Example 3

[0113] The composition of the present invention consists of the following components in parts by weight: 1 part superoxide dismutase, 1 part saffron extract, and 0.5 parts dipotassium glycyrrhizate.

[0114] The compositions prepared in the above embodiments and comparative examples can be used in skincare products. Skincare products include, but are not limited to, toners, serums, lotions, creams, and lyophilized powders. During preparation, in addition to the above compositions, the skincare products also include a matrix necessary for various dosage forms. The matrix can be selected from conventional matrices used in existing technologies for different dosage forms, and the skincare products can be prepared using conventional methods from existing technologies.

[0115] To verify the superior effects of the composition containing anti-blue light and anti-aging microspheres and dipotassium glycyrrhizate of the present invention, the compositions prepared in the above examples and comparative examples were formulated into anti-blue light and anti-aging serums. A skincare product that achieves anti-blue light damage through multi-pathway synergistic action is specifically a serum.

[0116] The basic formula of the anti-blue light anti-aging serum and the content of each composition in the anti-blue light anti-aging serum are shown in Tables 1 and 2 below.

[0117] Table 1. Proportions of each component in the serum formula.

[0118]

[0119]

[0120] Examples 1-3, Comparative Examples 1-3, and Blank Example 1

[0121] The only difference between Example 2 and Example 1 is the addition of each active ingredient, and the only difference between Example 3 and Example 1 is the reduction of each active ingredient.

[0122] The difference between Comparative Examples 1-2 and Example 3 is that only one key composition component is missing. The difference between Comparative Example 3 and Example 3 is that the key components, anti-blue light and anti-aging microspheres, are replaced with superoxide dismutase and saffron extract. The difference between Blank Example 1 and Example 3 is that all key composition components are missing.

[0123] Table 2. Content of the anti-blue light and anti-aging composition in the anti-blue light and anti-aging serum.

[0124]

[0125] The essence is prepared by the following method:

[0126] a. Aqueous phase: Place water in a container equipped with a stirring device and stir. After fully wetting and mixing the other components of phase A, add them to the water and stir thoroughly until completely dissolved. Heat to 80-85℃.

[0127] b. Oil phase; Mix the components of phase B and heat to 80-85℃, stirring until completely dissolved;

[0128] c. Emulsification: Add the oil phase obtained in step b to the aqueous phase obtained in step a, stir at 350-400 rpm for 3-5 minutes, and then homogenize at 3000-4000 rpm for 3-10 minutes.

[0129] d. Add each component to phase C, stir at 350-400 rpm for 3-5 minutes, then homogenize at 3000-4000 rpm for 3-5 minutes, and stir while cooling to 45-48℃;

[0130] e. Add the components from phase E, stir at 300-400 rpm for 3 minutes, and then homogenize at 3000-4000 rpm for 3-5 minutes;

[0131] f. After cooling to 35℃, add phase D, stir at 300-400 rpm for 3 minutes, then homogenize at 3000-4000 rpm for 3-5 minutes to obtain the essence. Before filling, filter to sterilize and test for bacterial content, toxins, immunogenicity, etc. for quality control.

[0132] All of the above steps are performed under strict aseptic conditions.

[0133] The characterization and performance data of the products in the examples and comparative examples are as follows.

[0134] Efficacy evaluation test

[0135] In vitro cell experiments

[0136] Table 3. Distribution ratio of raw materials for cell experiments

[0137]

[0138] 1. Tyrosinase inhibitory activity test

[0139] 1) Experimental materials

[0140] Cell line: Human immortalized keratinocytes (HaCaT) were cultured in DMEM medium containing 10% fetal bovine serum (FBS) at 37°C and 5% CO2.

[0141] Positive control: kojic acid solution (100 μg / mL).

[0142] Tyrosinase solution: purity ≥99%, dissolved in 0.1M phosphate buffer (PBS, pH 6.8) to prepare an enzyme solution of 1 mg / mL.

[0143] Substrate solution: L-DOPA (10 mM).

[0144] Cellular experimental materials: Experimental Examples 1-3, Experimental Comparative Examples 1-3.

[0145] Other reagents: PBS buffer (0.1M, pH 6.8), anhydrous ethanol, distilled water, etc.

[0146] Instruments and equipment: spectrophotometer, 96-well plate, pipette, constant temperature water bath, etc.

[0147] 2) Experimental Procedure

[0148] Cell culture and processing:

[0149] a. HaCaT cells were seeded in 96-well plates (density 5 × 10⁶ cells / well). 3 Add 100 μL of culture medium to each well and incubate the cells in a CO2 incubator at 37°C for 24 hours.

[0150] b. Aspirate the culture medium and gently wash the cells twice with PBS buffer to remove residual culture medium and unattached cells;

[0151] c. Add the cell experiment raw material group samples, positive control group (kojic acid solution) and blank group (without any additives) to a 96-well plate, with 3 replicates for each group;

[0152] d. Incubate the 96-well plate in a CO2 incubator for 48 hours.

[0153] Tyrosinase reaction:

[0154] a. Remove the culture medium after incubation and wash the cells twice with PBS buffer;

[0155] b. Add 50 μL of the cell experimental raw material sample of the present invention or the positive control drug solution to each well of a 96-well plate;

[0156] c. Add 50 μL of 1 mg / mL tyrosinase solution to each well;

[0157] d. Add 100 μL of 0.1 M PBS buffer to each well to make the total reaction volume 200 μL;

[0158] e. Set up a blank control group (only buffer solution is added, without sample and enzyme) and an enzyme control group (only enzyme solution and buffer solution are added, without sample);

[0159] f. Incubate the 96-well plate in a 37°C water bath for 10 minutes to allow the reaction system to reach equilibrium;

[0160] g. Add 50 μL of 10 mM L-DOPA solution to each well and mix quickly;

[0161] h. Continue incubation at 37°C for 30 minutes to allow tyrosinase to catalyze the production of dopaquinone and melanin from L-DOPA.

[0162] Absorbance measurement:

[0163] a. After the reaction is complete, remove the 96-well plate and cool it to room temperature;

[0164] b. Use a spectrophotometer to measure the absorbance of each well at a wavelength of 475 nm;

[0165] c. Using the experimental blank as a reference, calculate the inhibition rate.

[0166] 3) Detection indicators

[0167] Inhibition rate calculation:

[0168] Inhibition rate = (1-A) 样品 -A 空白 / A 酶对照 -A 空白 )×100%

[0169] Among them, A 样品 A represents the absorbance value of the sample group. 空白 A represents the absorbance value of the blank control group. 酶对照 The absorbance values ​​are for the enzyme control group. The test results are shown in Table 4 below.

[0170] Table 4 Tyrosinase Inhibition Rate

[0171]

[0172]

[0173] According to the data in Table 4, the tyrosinase inhibition rates of Experiments 1-3 (containing anti-blue light anti-aging microspheres and dipotassium glycyrrhizate composition) were 83.14%, 86.59%, and 78.03%, respectively, significantly higher than those of Comparative Example 1 (without dipotassium glycyrrhizate, 52.34%), Comparative Example 2 (without anti-blue light anti-aging microspheres, 51.17%), and Comparative Example 3 (free SOD and saffron extract, 63.72%). The inhibition rate of Experiment 2 (86.59%) was close to that of the positive control kojic acid (88.31%), indicating that the composition of the present invention achieves highly efficient tyrosinase activity blocking through the core-shell microsphere encapsulation technology (protecting SOD activity and slow-release saffron extract) and the targeted inhibition synergistic effect of dipotassium glycyrrhizate. Specifically, the microsphere system enhances free radical scavenging and blue light absorption capabilities by stably delivering SOD and saffron extract, while dipotassium glycyrrhizate directly chelates the metal ions at the active center of tyrosinase, jointly inhibiting melanin production. In contrast, Comparative Example 3, lacking microsphere protection, resulted in degradation of active ingredients, with a significantly lower inhibition rate than the experimental example, further validating the crucial role of microsphere technology in maintaining ingredient stability and efficacy.

[0174] 2. Cellular ROS Inhibition Experiment

[0175] (1) Cell seeding: Human keratinocytes HaCat were seeded into the wells of a 24-well culture plate at a density of 3×10⁵ cells / well, and then incubated in a CO₂ cell culture incubator for 24 hours.

[0176] (2) According to the experimental group, 50 μL of each test substance was added to the cells after inoculation in step (1) and cultured for 24 h. The experimental groups are as follows: blank control group (no serum culture medium, no UVB irradiation later), damage model group (no serum culture medium, UVB irradiation later), positive control group (vitamin C 200 ug / mL), experimental examples 1-3 and experimental comparison examples 1-3 (sample solution prepared with 200 ug / mL serum culture medium).

[0177] (3) Except for the blank control group, the remaining cells were irradiated with UVB (2J / cm2).

[0178] (4) Discard the culture medium, wash and fix the cells, and add the working concentration of the reactive oxygen species fluorescent probe DCFH-DA reagent to the cells for co-incubation according to the reagent operation instructions.

[0179] (5) After incubation, using the blank control group as a reference, the cells were photographed under an inverted fluorescence microscope, and the fluorescence intensity was counted. The rate of change in intracellular fluorescence intensity was calculated as follows: Rate of change in intracellular fluorescence intensity = (Sample fluorescence intensity - Fluorescence intensity of the damage model group) / Fluorescence intensity of the damage model group × 100%

[0180] Table 5. Rate of change in intracellular fluorescence intensity

[0181]

[0182]

[0183] According to the data in Table 5, the intracellular fluorescence intensity change rates of Experimental Examples 1-3 (composition containing anti-blue light anti-aging microspheres and dipotassium glycyrrhizate) were -54.19%, -56.43%, and -50.78%, respectively, which were significantly higher than those of Comparative Example 1 (without dipotassium glycyrrhizate, -36.15%), Comparative Example 2 (without anti-blue light anti-aging microspheres, -34.62%), and Comparative Example 3 (free SOD and saffron extract, -45.17%). This indicates that the composition of the present invention can significantly inhibit UVB-induced intracellular ROS generation, effectively scavenge free radicals, and thus reduce oxidative damage. Among them, Experimental Example 2 showed the most significant inhibitory effect, close to that of the positive control group (vitamin C, -57.76%). In addition, compared with the free active ingredients, the active ingredients in the composition of the present invention are more stable under the protection of microspheres and can more effectively exert antioxidant effects, further verifying the significant advantages of the composition of anti-blue light anti-aging microspheres and dipotassium glycyrrhizate in terms of anti-oxidation and anti-photodamage. The comparison results show that, compared with using only one of the anti-blue light anti-aging microspheres and dipotassium glycyrrhizate, the anti-oxidation and anti-photodamage effects are more obvious when both are used, indicating that the anti-blue light anti-aging microspheres and dipotassium glycyrrhizate have a synergistic effect.

[0184] 3. Matrix metalloproteinase (MMP) inhibition assay

[0185] 1) Seed human fibroblasts at a density of 10³-10⁴ cells / well in 24-well plates, add 100 μL of LMEM medium, and incubate the cells in a CO₂ incubator at 37°C for 24 hours.

[0186] 2) Add 50 μL of each of Experimental Examples 1-3, Comparative Examples 1-3 and Blank Example 1 to the plate and incubate in an incubator for 48 hours.

[0187] 3) Collect cell culture supernatant and use an ELISA kit to add the supernatant of Experimental Examples 1-3, Comparative Examples 1-3 and Blank Example 1 to the corresponding reaction wells in the ELISA plate for subsequent ELISA analysis.

[0188] The ELISA kit includes MMP-1 and MMP-3 specific antibodies and standards.

[0189] 4) Measure the absorbance at 450 nm using an ELISA reader, and calculate the concentrations of MMP-1 and MMP-3 in the sample based on the standard curve.

[0190] 5) Using experimental blank example 1 as a reference, calculate the inhibition rate of MMP-1 and MMP-3 synthesis by experimental examples 1-3 and blank example 1-3. Synthesis inhibition rate (%) = (absorbance of test group - absorbance of experimental blank example 1) / absorbance of experimental blank example 1 × 100%; the test results are shown in Table 6-7 below.

[0191] Table 6 MMP-1 Synthesis Inhibition Rate

[0192]

[0193]

[0194] Table 7 MMP-3 Synthesis Inhibition Rate

[0195] sample MMP-3 synthesis inhibition rate % Experimental Example 1 40.87 Experiment Example 2 42.11 Experimental Example 3 35.27 Comparative Example 1 24.63 Comparative Example 2 24.18 Comparative Example 3 29.89

[0196] According to the data in Tables 6 and 7, the MMP-1 synthesis inhibition rates of Examples 1-3 were 43.21%, 45.77%, and 38.59%, respectively, and the MMP-3 synthesis inhibition rates were 40.87%, 42.11%, and 35.27%, respectively. This indicates that the composition of the present invention can significantly inhibit UVB-induced MMP-1 and MMP-3 synthesis, effectively reduce the degradation of collagen and elastin fibers, thereby delaying skin laxity and wrinkle formation. Among them, Example 2 showed the most significant inhibitory effect, approaching the level of the positive control group. In contrast, the MMP-1 synthesis inhibition rates of Comparative Example 1 (without dipotassium glycyrrhizate) and Comparative Example 2 (without anti-blue light anti-aging microspheres) were 27.27% and 25.32%, respectively, and the MMP-3 synthesis inhibition rates were 24.63% and 24.18%, respectively, showing significantly insufficient inhibitory ability. Furthermore, the synthesis inhibition rates of experimental comparative example 3 for MMP-1 and MMP-3 were 33.83% and 29.89%, respectively, which were significantly lower than those of experimental examples 1-3. This fully demonstrates that active ingredients without microsphere protection cannot achieve stable and efficient MMP inhibition effects. Microsphere encapsulation technology significantly improves the stability and efficacy of active ingredients, effectively protects dermal collagen and elastic fibers, and delays photoaging.

[0197] Characterization data and effect data of the products in the examples and comparative examples

[0198] Human efficacy test

[0199] (1) Test samples: the essence of Examples 1-3, Comparative Examples 1-3 and Blank Example 1 as described in Tables 1 and 2.

[0200] (2) Test subjects: 70 healthy women (28-55 years old) were randomly divided into 7 groups of 10 each, and each group used the same formula product. Written informed consent forms were signed. Before enrollment, subjects were asked a series of questions about their medical history and health status according to the inclusion and exclusion criteria. At the same time, the skin of the test site was assessed for conformity and skin color was tested for screening, and the results were recorded.

[0201] Environmental conditions: The visual assessment and instrument testing were conducted in an environment with a temperature of 21±1℃ and a relative humidity of 50±10%RH. The visual assessment was conducted under constant lighting conditions (fluorescent tubes or LED lights with a color temperature of 5500~6500K). Subjects were required to adapt to these environmental conditions for at least 30 minutes before assessment and testing could be performed.

[0202] (3) Test method: Apply the sample twice a day, morning and evening, for 4 weeks. Apply an appropriate amount of the test sample to the cheeks. Apply sunscreen to the entire face every morning.

[0203] Results were tested at the following three time periods: before sample use (D0), 2 weeks after sample use (W1), and 4 weeks after sample use (W4).

[0204] (4) The test results can be calculated using the following formula:

[0205] Rate of change = (Analysis value after product use - Analysis value before product use) ÷ Analysis value before product use × 100%

[0206] 1) Skin roughness

[0207] Antera 3D was used to test skin roughness; data is shown in Table 8. A smaller analytical value and a smaller rate of change indicate an improvement in skin roughness.

[0208] Table 8. Change Rate of Skin Roughness

[0209] sample Use for two weeks % Use for four weeks % Example 1 -8.56 -14.25 Example 2 -9.24 -15.84 Example 3 -7.52 -12.06 Comparative Example 1 -6.21 -9.61 Comparative Example 2 -6.07 -9.55 Comparative Example 3 -6.95 -11.08 Blank example 1 -3.12 -6.23

[0210] Based on the data in Table 8 and Figures 2 to 4The skin roughness change rates of Examples 1-3 were -8.56%, -9.24%, and -7.52% after 2 weeks of use, and -14.25%, -15.84%, and -12.06% after 4 weeks of use, respectively. This indicates that the composition of the present invention can significantly improve skin roughness, and the improvement effect becomes more obvious with the extension of use time, making the skin smoother and more delicate. Compared with the examples, Comparative Examples 1 and 2 lacked key components (dipotassium glycyrrhizate or anti-blue light anti-aging microspheres), and their improvement effects were significantly inferior to those of the examples. This indicates that dipotassium glycyrrhizate and anti-blue light anti-aging microspheres are key components in the composition of the present invention that play a role in improving skin roughness, and both are indispensable. In addition, although Comparative Example 3 contained some active ingredients (superoxide dismutase and saffron extract), its improvement effect was still inferior to that of the examples due to the lack of microsphere technology protection, further demonstrating the importance of microsphere technology in improving the stability and efficacy of active ingredients. Experiments show that compared with using only one of the anti-blue light anti-aging microspheres and dipotassium glycyrrhizate, the combined use of anti-blue light anti-aging microspheres and dipotassium glycyrrhizate results in a more significant improvement in skin roughness. The composite system of dipotassium glycyrrhizate and anti-blue light anti-aging microspheres can significantly improve skin smoothness through stable active ingredients and targeted delivery, and its synergistic effect is far superior to the application of single ingredients.

[0211] 2) Number of wrinkles under the eyes

[0212] The EvaFACE rapid optical imaging system was used to collect data on the number of under-eye wrinkles; the data is shown in Table 9. A smaller analytical value and a smaller rate of change indicate an improvement in the number of under-eye wrinkles.

[0213] Table 9. Change Rate of the Number of Under-Eye Wrinkles

[0214]

[0215]

[0216] Based on the data in Table 9 and Figures 5 to 7The change rates of under-eye wrinkles in Examples 1-3 were -9.03%, -9.89%, and -7.94% after 2 weeks of use, and -11.67%, -12.51%, and -10.16% after 4 weeks of use, respectively. This indicates that the composition of the present invention can significantly reduce the number of under-eye wrinkles, and the improvement effect is more obvious with the extension of use time, effectively alleviating skin wrinkles and making the skin around the eyes smoother and firmer. Compared with the examples, Comparative Examples 1 and 2 lacked key components (dipotassium glycyrrhizate or anti-blue light anti-aging microspheres), and their improvement effects were significantly inferior to those of the examples. This indicates that dipotassium glycyrrhizate and anti-blue light anti-aging microspheres are key components in the composition of the present invention that play a role in improving under-eye wrinkles, and both are indispensable. In addition, although Comparative Example 3 contains some active ingredients (superoxide dismutase and saffron extract), its improvement effect is still inferior to that of the examples due to the lack of microsphere technology protection, further demonstrating the importance of microsphere technology in improving the stability and efficacy of active ingredients. Experiments show that, compared to using only one of the two anti-blue light anti-aging microspheres and dipotassium glycyrrhizate, the combined use of anti-blue light anti-aging microspheres and dipotassium glycyrrhizate significantly improves the smoothness and firmness of the skin around the eyes. The composite system of dipotassium glycyrrhizate and anti-blue light anti-aging microspheres can significantly improve the smoothness and firmness of the skin around the eyes through stable active ingredients and targeted delivery; its synergistic effect is far superior to the application of a single ingredient.

[0217] In summary, the multifunctional anti-blue light and anti-aging microsphere composition, its preparation method, applications, and skincare products proposed in this invention utilize hyaluronic acid microspheres as the core carrier. Their three-dimensional network structure creates a stable protective environment, effectively solving the industry problem of poor stability of active ingredients and significantly improving their stability. On one hand, by physically encapsulating and immobilizing superoxide dismutase (SOD) through an ionic cross-linking network, its half-life is significantly extended, ensuring the long-term effectiveness of free radical scavenging. On the other hand, the sustained-release properties of the microspheres allow for precise controlled release of SOD and saffron extract into the skin, penetrating deep to exert their antioxidant effects. Dipotassium glycyrrhizate, through microsphere targeted delivery technology, precisely targets areas of active inflammation, inhibiting tyrosinase activity and chelating metal ions to block the melanin synthesis signaling pathway at its source, achieving targeted repair.

[0218] This invention constructs a unique core-shell protection system, forming a dual blue light barrier of "oxidation neutralization-light absorption." The core layer uses ion cross-linking technology to fix SOD, constructing a frontline defense against oxidative stress and targeting and eliminating excess free radicals induced by blue light. The shell layer utilizes the conjugated structural properties of crocin to form a specific light absorption barrier, converting high-energy blue light energy into harmless heat energy and reducing energy transmission to the dermis. This core-shell structure overcomes the limitations of single-dimensional protection, achieving synergistic effects of photoprotection and antioxidation. Experimental data show that this system increases the inhibition rate of intracellular ROS generation, significantly enhances the inhibition rate of MMP-1 / MMP-3 synthesis, and effectively delays collagen degradation.

[0219] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0220] The description and application of the present invention herein are illustrative and not intended to limit the scope of the invention to the embodiments described above. Effects or advantages involved in the embodiments may not be apparent due to various factors, and the description of effects or advantages is not intended to limit the embodiments. Variations and modifications of the embodiments disclosed herein are possible, and various substitutions and equivalents of the components in the embodiments are well known to those skilled in the art. It should be apparent to those skilled in the art that the invention can be implemented in other forms, structures, arrangements, proportions, and with other components, materials, and parts without departing from the spirit or essential characteristics of the invention. Other variations and modifications can be made to the embodiments disclosed herein without departing from the scope and spirit of the invention.

Claims

1. A multifunctional microsphere composition for anti-blue light and anti-aging, characterized in that, The microsphere composition comprises: anti-blue light and anti-aging microspheres and dipotassium glycyrrhizate, wherein the mass ratio of the anti-blue light and anti-aging microspheres to dipotassium glycyrrhizate is (0.1-8):(0.01-3).

2. The anti-blue light and anti-aging multifunctional microsphere composition according to claim 1, characterized in that: The mass ratio of the anti-blue light and anti-aging microspheres to dipotassium glycyrrhizate is (0.1-5):(0.01-1).

3. The anti-blue light and anti-aging multifunctional microsphere composition according to claim 1, characterized in that: The anti-blue light anti-aging microspheres include superoxide dismutase and saffron extract; the mass ratio of superoxide dismutase to saffron extract in the anti-blue light anti-aging microspheres is (5-15):(5-12).

4. The anti-blue light and anti-aging multifunctional microsphere composition according to claim 1, characterized in that: The preparation method of the anti-blue light and anti-aging microspheres includes: Step S1: Constructing a core layer activity protection system; Raw material mixing steps: Dissolve 1.0-2.0 parts by weight of superoxide dismutase (SOD), 5.0-6.0 parts by weight of sodium alginate, and 0.3-0.5 parts by weight of maltodextrin in pre-cooled biocompatible buffer A with a pH of 6-7, and stir to form a homogeneous core phase solution; Ion crosslinking molding step: The core phase solution is injected into a water-soluble calcium salt solution, stirred, and solidified to generate a porous gel core; free calcium ions are removed by washing with deionized water to obtain core layer gel particles with uniform particle size. Step S2: Functionalization of blue light absorption in the shell layer; Preparation steps of self-assembled complex: Dissolve 3.0-4.0 parts by weight of hyaluronic acid in a weakly acidic buffer solution B with a pH of 4-5. After dissolving by stirring in a water bath, add 1.0-1.5 parts by weight of saffron extract and 0.1-0.2 parts by weight of tea polyphenols. Sonicate in the dark and let stand for a set time to form an aqueous shell solution. Interfacial polymerization coating steps: The core layer gel particles are dispersed in a hydrophobic ester solvent, and 0.1-0.15 parts by weight of PEG-40 hydrogenated castor oil is added as an emulsifier. Shear emulsification is performed to form a W / O primary emulsion. The shell layer aqueous solution is slowly added to the primary emulsion, and shear emulsification is continued to form a W / O / W secondary emulsion. The emulsion is then transferred to a water bath, and the shell coating is completed through reaction to form microspheres. Step S3: Crosslinking curing and surface modification steps; Bio-crosslinking enhancement step: Immerse the microspheres in 0.05-0.2% w / v biocompatible cationic polymer solution C, and slowly shake to enhance shell stability through electrostatic interaction, followed by washing with buffer solution with pH 7.1-8; Visible light responsive curing step: The microspheres are photocured using a light source and stirred simultaneously to ensure uniform cross-linking.

5. A skincare product, characterized in that: The skincare product comprises the anti-blue light and anti-aging multifunctional microsphere composition according to any one of claims 1 to 4.

6. A method for preparing anti-blue light and anti-aging microspheres, characterized in that, The preparation method includes the following steps: Step S1: Constructing a core layer activity protection system; Raw material mixing steps: Dissolve 1.0-2.0 parts by weight of superoxide dismutase (SOD), 5.0-6.0 parts by weight of sodium alginate, and 0.3-0.5 parts by weight of maltodextrin in pre-cooled biocompatible buffer A with a pH of 6-7, and stir to form a homogeneous core phase solution; Ion crosslinking molding step: The core phase solution is injected into a water-soluble calcium salt solution, stirred, and solidified to generate a porous gel core; Free calcium ions were removed by washing with deionized water to obtain core layer gel particles with uniform particle size; Step S2: Functionalization of blue light absorption in the shell layer; Preparation steps of self-assembled complex: Dissolve 3.0-4.0 parts by weight of hyaluronic acid in a weakly acidic buffer solution B with a pH of 4-5. After dissolving by stirring in a water bath, add 1.0-1.5 parts by weight of saffron extract and 0.1-0.2 parts by weight of tea polyphenols. Sonicate in the dark and let stand for a set time to form an aqueous shell solution. Interfacial polymerization coating steps: The core layer gel particles are dispersed in a hydrophobic ester solvent, and 0.1-0.15 parts by weight of PEG-40 hydrogenated castor oil is added as an emulsifier. Shear emulsification is performed to form a W / O emulsion. The shell layer aqueous solution is slowly added to the primary emulsion, and shear emulsification is continued to form a W / O / W complex emulsion. The mixture is then transferred to a water bath, and the shell coating is completed through reaction to form microspheres. Step S3: Crosslinking curing and surface modification steps; Bio-crosslinking enhancement step: Immerse the microspheres in 0.05% to 0.2% w / v biocompatible cationic polymer solution C, and slowly shake to enhance shell stability through electrostatic interaction, followed by washing with buffer solution with pH 7.1 to 8; Visible light responsive curing step: The microspheres are photocured using a light source and stirred simultaneously to ensure uniform cross-linking.

7. The method for preparing anti-blue light and anti-aging microspheres according to claim 6, characterized in that: The biocompatible buffer A includes at least one of phosphate buffer, HEPES buffer, and MES buffer. The water-soluble calcium salt solution is a water-soluble calcium salt solution, which includes at least one of calcium lactate, calcium chloride, and calcium gluconate; The weakly acidic buffer B includes at least one of citrate buffer and acetate-sodium acetate buffer; The hydrophobic ester solvent includes at least one of glyceryl caprylate or isononyl isononanoate; The biocompatible cationic polymer solution C includes at least one of polylysine and ε-polylysine solution.

8. A method for preparing a multifunctional microsphere composition for anti-blue light and anti-aging, characterized in that, The preparation method includes: preparing anti-blue light anti-aging microspheres, mixing anti-blue light anti-aging microspheres and dipotassium glycyrrhizate in a set mass ratio to obtain an anti-blue light anti-aging microsphere composition; the mass ratio of the anti-blue light anti-aging microspheres to dipotassium glycyrrhizate is (0.1-8):(0.01-3).

9. The use of the anti-blue light and anti-aging multifunctional microsphere composition according to any one of claims 1 to 4 in the preparation of skin care products.

10. The application according to claim 9, characterized in that: The skincare product is one of the following: skincare water, serum, lotion, face cream, or freeze-dried powder.

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