A whitening composition and uses thereof, cosmetic

Abstract

The application belongs to the technical field of cosmetics, and discloses a whitening composition, application and cosmetics thereof; the whitening composition contains the following active components in parts by mass: 16-24 parts of coix seed fermentation liquor, 8-12 parts of extract of peony, 22-38 parts of 4-butylresorcinol solution and 2-8 parts of zinc gluconate; through the synergistic effect among the four components, the activity of tyrosinase can be effectively inhibited, the synthesis of cell melanin can be effectively inhibited, and the whitening effect is good.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and discloses a whitening composition, use thereof and a cosmetic. BACKGROUND

[0002] With the continuous improvement of people's concern for their own image and skin health, whitening skin care has become an important demand direction of the cosmetic market. At present, the global market size of whitening skin care products continues to expand, and consumers' demand for safe, efficient and mild whitening products is increasingly urgent.

[0003] At present, the common whitening products on the market mainly achieve the whitening effect through inhibiting tyrosinase activity, preventing melanin transport, promoting melanin metabolism and other ways. However, most of the existing whitening products still have some problems: some whitening ingredients, such as hydroquinone, have significant whitening effect, but have strong cytotoxicity, and long-term use may cause irreversible damage to the skin, and even cause skin allergy, aggravation of pigmentation and other adverse reactions; for example, kojic acid, which has poor stability under high temperature and light conditions, is easy to decompose and deteriorate, affecting the shelf life and whitening effect of the product; when used at high concentration, nicotinamide may cause stinging, redness and other intolerance in some sensitive skin groups. In addition, the whitening ingredients of some whitening products are relatively single, and it is often difficult to achieve the ideal whitening effect.

[0004] Therefore, the technical problem to be solved by the present application is how to prepare a new whitening composition. SUMMARY

[0005] The present application aims to provide a whitening composition comprising coix seed fermentation liquor, paeony extract combination factor, 4-butylresorcinol solution and zinc gluconate. When these components are used together, they have obvious synergistic effect and can exert excellent whitening effect.

[0006] Meanwhile, the present application also provides a use of the whitening composition and a cosmetic.

[0007] To achieve the above-mentioned purpose, the present application provides the following technical scheme:

[0008] A whitening composition, comprising the following active components in parts by mass: coix seed fermentation liquor 16-24 parts, paeony extract combination factor 8-12 parts, 4-butylresorcinol solution 22-38 parts and zinc gluconate 2-8 parts.

[0009] Preferably, the whitening composition further comprises 0.1-1.5 parts of decarboxylated carnosine hydrochloride and 0.05-2.5 parts of polygonatum extract combination factor by mass fraction.

[0010] Preferably, the whitening composition further comprises water 21-32.75 parts by mass, butylene glycol 4-6 parts by mass, 1,2-hexanediol 0.4-0.6 parts by mass, and p-hydroxyacetophenone 0.4-0.6 parts by mass.

[0011] Preferably, the co-factor of the extract of the root of Paeonia lactiflora comprises, by total mass 100%, 30wt% of the extract of the seed of Coix lacryma-jobi, 68wt% of glycerol, and 2wt% of 1,2-hexanediol.

[0012] Preferably, the co-factor of the extract of the root of Paeonia lactiflora comprises, by total mass 100%, 30wt% of the extract of the root of Paeonia lactiflora, 30wt% of butylene glycol, 39wt% of water, 0.5wt% of 1,2-hexanediol, and 0.5wt% of p-hydroxyacetophenone.

[0013] Preferably, the 4-butylresorcinol solution comprises, by total mass 100%, 10wt% of 4-butylresorcinol, 40wt% of hydroxypropyl cyclodextrin, 44wt% of water, 5wt% of 1,3-propanediol, 0.5wt% of 1,2-hexanediol, and 0.5wt% of p-hydroxyacetophenone.

[0014] In addition, the present application discloses the use of the whitening composition as described above for preparing a cosmetic product.

[0015] Finally, the present application also discloses a cosmetic product comprising 1wt%-20wt% of the whitening composition as described above.

[0016] Preferably, the cosmetic product comprises 2.5wt%-20wt% of the whitening composition as described above.

[0017] Preferably, the cosmetic product is one of a cosmetic water, an emulsion, a cream, an essence, a mask, a cleanser, and a shower gel.

[0018] Compared with the prior art, the present application has the following advantages:

[0019] The whitening composition prepared by the present application comprises the co-factor of the extract of the seed of Coix lacryma-jobi, the co-factor of the extract of the root of Paeonia lactiflora, the 4-butylresorcinol solution, and zinc gluconate, and through the synergistic effect of the four components, the activity of tyrosinase can be effectively inhibited, and the synthesis of cellular melanin can be effectively inhibited, thus having a good whitening effect. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 A column chart showing the effect of different concentrations of the detection sample of Example 1 on the activity of tyrosinase;

[0021] Figure 2Microscope photos of the influence of different concentrations of the test sample of Example 1 on the melanin synthesis of B16 cells;

[0022] Figure 3 Bar chart of the influence of different concentrations of the test sample of Example 1 on the melanin synthesis of B16 cells. DETAILED DESCRIPTION

[0023] The technical solutions of the present application will be described clearly and completely below in combination with the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, but not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application. It should be noted that, if the specific conditions are not mentioned in the embodiments, the conventional conditions or the conditions suggested by the manufacturer are adopted. If the manufacturers of the reagents or instruments are not mentioned, they are all conventional products that can be purchased in the market.

[0024] Material commodity information:

[0025] Coixan fermentate: purchased from Guangzhou Jikemai Life Science Co., Ltd.;

[0026] Paeonia extract combination factor: purchased from Shanghai Ruixi Industry Co., Ltd.;

[0027] 4-Butylresorcinol: purchased from Youyan Biological Technology (Huzhou) Co., Ltd.;

[0028] Decarboxylated myo-inositol hydrochloride: purchased from Guangzhou Baiceste Chemical Co., Ltd.;

[0029] Rhizoma polygonati extract combination factor: purchased from Ruixi (Guangzhou) Trade Co., Ltd.;

[0030] Zinc gluconate: purchased from Zhengzhou Ruipu Biological Engineering Co., Ltd.

[0031] In the present application, the following "Sanmu core" is the trade name of the whitening composition provided by the present application, unless otherwise specified.

[0032] The components in the coixan fermentate, paeonia extract combination factor, 4-butylresorcinol solution and rhizoma polygonati extract combination factor used in the following embodiments are shown in Tables 1-4.

[0033] Table 1 Coixan fermentate component table

[0034]

[0035] Table 2 Paeonia extract combination factor component table

[0036]

[0037] Table 3 Component table of 4-butylresorcinol solution

[0038]

[0039] Table 4 Component table of rhizoma polygonati extract combination factor

[0040]

[0041] Example 1

[0042] The present example provides a whitening composition, which comprises the following components and contents according to 100 parts by total mass: coix seed fermentation broth 20 parts, paeonia extract combination factor 10 parts, 4-butylresorcinol solution 30 parts, zinc gluconate 5 parts, water 29 parts, butanediol 5 parts, 1,2-hexanediol 0.5 parts, and p-hydroxyacetophenone 0.5 parts.

[0043] Example 2

[0044] The present example provides a whitening composition, which comprises the following components and contents according to 100 parts by total mass: coix seed fermentation broth 16 parts, paeonia extract combination factor 12 parts, 4-butylresorcinol solution 38 parts, zinc gluconate 2 parts, water 25 parts, butanediol 6 parts, 1,2-hexanediol 0.4 parts, and p-hydroxyacetophenone 0.6 parts.

[0045] Example 3

[0046] The present example provides a whitening composition, which comprises the following components and contents according to 100 parts by total mass: coix seed fermentation broth 24 parts, paeonia extract combination factor 8 parts, 4-butylresorcinol solution 22 parts, zinc gluconate 8 parts, water 33 parts, butanediol 4 parts, 1,2-hexanediol 0.6 parts, and p-hydroxyacetophenone 0.4 parts.

[0047] Example 4

[0048] The present example provides a whitening composition, which comprises the following components and contents according to 100 parts by total mass: coix seed fermentation broth 22 parts, paeonia extract combination factor 11 parts, 4-butylresorcinol solution 25 parts, zinc gluconate 4 parts, water 31 parts, butanediol 6 parts, 1,2-hexanediol 0.6 parts, and p-hydroxyacetophenone 0.4 parts.

[0049] Example 5

[0050] The embodiment provides a whitening composition, which comprises the following components and contents according to 100 parts of total mass fraction: 20 parts of coix seed fermentation liquor, 10 parts of paeony extract combination factor, 30 parts of 4-butylresorcinol solution, 1.5 parts of decarboxylated myo-inositol hydrochloride, 5 parts of zinc gluconate, 27.5 parts of water, 5 parts of butanediol, 0.5 part of 1,2-hexanediol, and 0.5 part of p-hydroxyacetophenone.

[0051] Embodiment 6

[0052] The embodiment provides a whitening composition, which comprises the following components and contents according to 100 parts of total mass fraction: 20 parts of coix seed fermentation liquor, 10 parts of paeony extract combination factor, 30 parts of 4-butylresorcinol solution, 1.5 parts of decarboxylated myo-inositol hydrochloride, 5 parts of zinc gluconate, 27.5 parts of water, 5 parts of butanediol, 0.5 part of 1,2-hexanediol, and 0.5 part of p-hydroxyacetophenone.

[0053] Embodiment 7

[0054] The embodiment provides a whitening composition, which comprises the following components and contents according to 100 parts of total mass fraction: 20 parts of coix seed fermentation liquor, 10 parts of paeony extract combination factor, 30 parts of 4-butylresorcinol solution, 1.5 parts of decarboxylated myo-inositol hydrochloride, 5 parts of zinc gluconate, 27.5 parts of water, 5 parts of butanediol, 0.5 part of 1,2-hexanediol, and 0.5 part of p-hydroxyacetophenone.

[0055] Comparative Examples 1-4

[0056] The whitening composition provided by Comparative Examples 1-4 comprises the components and mass fractions shown in Table 5.

[0057] Table 5 Component Table

[0058] Fermented liquor of coix seed Factor of peony extract 4-Butylresorcinol solution Zinc gluconate Water Butylene glycol 1,2-Hexanediol p-Hydroxyacetophenone Total parts Comparative Example 1 0 15 45 5 29 5 0.5 0.5 100 Comparative Example 2 23 0 37 5 29 5 0.5 0.5 100 Comparative Example 3 40 20 0 5 29 5 0.5 0.5 100 Comparative Example 4 20 10 30 0 34 5 0.5 0.5 100

[0059] The preparation method of the whitening composition of each embodiment and comparative example is as follows:

[0060] Each component is added into water in proportion, dissolved at 25 DEG C, and uniformly mixed to obtain the whitening composition.

[0061] Performance detection

[0062] 1. Experimental principle

[0063] Tyrosinase is a key enzyme in the melanin biosynthesis of skin, which acts on tyrosine and spontaneously undergoes a series of reactions to finally form melanin. Tyrosinase can catalyze the production of melanin from tyrosine in a buffer solution at pH 6.8, and the absorbance at 450 mm can be measured. Raw materials with tyrosinase activity inhibition can reduce the catalytic effect of tyrosinase, thereby reducing the absorbance. According to the change of absorbance, the inhibition of sample on tyrosinase activity can be evaluated. The production of melanin by melanocytes in the skin is the main cause of skin darkening and the formation of pigmented spots. Therefore, in this experiment, tyrosinase and melanocytes were used as test tools to evaluate the whitening effect of samples by changes in tyrosinase activity and melanin content after administration.

[0064] 2. Equipment and materials

[0065] 2.1 Equipment: 37℃ constant temperature incubator, multifunctional enzyme label instrument (TECAN, SPARK), microscope (Olympus);

[0066] 2.2 Cell line: B16 (mouse melanoma cells);

[0067] 2.3 Reagents: tyrosinase (Merck), L-tyrosine (Merck), kojic acid (purity ≥ 98.5%, source leaf), melanin staining kit (ferrous sulfate method) (Solabio), DMEM high glucose culture medium containing 10% FBS (complete culture medium).

[0068] 3. Test sample processing

[0069] Test sample group: The whitening composition samples prepared in Examples 1-7 and Comparative Examples 1-4 were filtered with a 0.22 um filter membrane, and the filtrate was collected as the test sample mother liquor, and then diluted with pure water to a volume concentration of 1.0%, 1.5%, 2.5%, 5.0%, 7.5%, and 10.0%.

[0070] Blank control group: pure water;

[0071] Positive control group: kojic acid (100 μg / mL).

[0072] 4. Test method

[0073] 4.1 Tyrosinase activity inhibition

[0074] In a 96-well plate, 50 μL of different concentrations of test sample diluent and 20 μL of tyrosinase solution with a concentration of 200 U / mL were added, and then shaken and mixed. After incubation at 37 ℃ for 10 min, 130 μL of L-tyrosine solution was added, and then shaken and mixed. After incubation at 37 ℃ for 15 min, the OD450nm value was detected, and the tyrosinase inhibition rate was calculated according to Formula 1.

[0075] Formula 1:

[0076]

[0077] In the formula:

[0078] T: sample tube absorbance value, i.e. the absorbance value of the solution after the sample reacts with tyrosinase;

[0079] T0: sample background absorbance value;

[0080] C: enzyme reaction tube absorbance value, i.e. the absorbance value of the reaction of tyrosinase and dopa without adding sample;

[0081] C0: solvent background absorbance value.

[0082] 4.1.1 Test results

[0083] The tyrosinase inhibition rate results are shown in Figure 1 Table 6 and Table 7.

[0084] Table 6 Tyrosinase inhibition rate

[0085]

[0086] From Figure 1 and Table 6, it can be seen that the inhibition rate of tyrosinase activity of Sanmu core (Example 1) is 8.23%, 11.36%, 30.22%, 45.76%, 78.27%, and 83.41% at 1.0%, 1.5%, 2.5%, 5.0%, 7.5%, and 10.0%, respectively, which has a significant difference compared with the blank control group, indicating that Sanmu core (Example 1) has a significant inhibitory effect on tyrosinase activity.

[0087] It can also be seen that the tyrosinase inhibition rate of the whitening composition provided by Example 1 is the highest when the content is 10.0%. Therefore, the compositions provided by Examples 2-7 and Comparative Examples 1-4 with a volume concentration of 10.0% were tested for tyrosinase activity inhibition, and the tyrosinase inhibition rates are shown in Table 7.

[0088] Table 7 Tyrosinase inhibition rate data table

[0089] Sample Tyrosinase inhibition rate (%) Example 2 82.65 Example 3 84.17 Example 4 83.42 Example 5 85.67 Example 6 85.24 Example 7 89.49 Comparative Example 1 60.38 Comparative Example 2 71.94 Comparative Example 3 68.75 Comparative Example 4 58.96

[0090] From the data in Table 6 and Table 7, it can be seen that Sanmu core has a significant inhibitory effect on tyrosinase activity, especially Example 7, which has an excellent whitening effect.

[0091] 4.2 B16 cell melanin synthesis inhibition test

[0092] 4.2.1 Cell preparation

[0093] B16 cells were cultured in complete medium at 37℃, 5% CO 2 The cell concentration was controlled to be 1.0 x 10 5 ~5.0 x 10 6 cells per 1 mL, and the cells were used for biological activity determination 24-36 hours after subculture.

[0094] 4.2.2 Safety concentration screening of the test substance

[0095] The cells were seeded at 3500 cells per well in a 96-well cell culture plate and adherently cultured for 24 hours. The old medium was removed, and complete medium containing different concentrations of the test substance was added and incubated for 24 hours. 0.5% MTT was added and incubated at 37℃, 5% CO 2 for 4 hours. The liquid in the culture plate was discarded, DMSO (dimethyl sulfoxide) was added, and the absorbance at 490 nm was determined using an enzyme marker, and the determination results were recorded.

[0096] 4.2.3 Efficacy detection

[0097] Cell plating: The cells were resuspended with fresh complete medium and seeded at 2500 cells per well in a 6-well cell culture plate and adherently cultured for 24 hours.

[0098] Sample preparation: The safety concentration of the test substance that was not toxic to the cells was selected, and blank control, test sample, and positive control groups were set up as follows:

[0099] Blank control group: pure water;

[0100] Test sample group: the whitening composition samples prepared in Examples 1-7 and Comparative Examples 1-4 were filtered with a 0.22 um filter membrane, and the filtrate was collected as the test sample mother liquor, and diluted with pure water to a volume concentration of 0.005%, 0.01%, 0.02%, and 0.03%;

[0101] Positive control group: kojic acid (50 μg / mL).

[0102] First medium change: the old medium was discarded, and the prepared solutions of each group were added to the 6-well cell culture plate, and the culture was continued.

[0103] Second medium change: 48 hours after the first medium change, the cell culture medium containing the blank control group, the test sample group (0.005%, 0.01%, 0.02%, and 0.03%), and the positive control group was replaced, and the culture was continued.

[0104] Third medium change: 72 hours after the second medium change, the new cell culture medium was replaced, and the culture was continued for 48-72 hours (cell fusion rate reached more than 90%).

[0105] Melanin content detection: according to the melanin staining kit (ferrous sulfate method) instructions for staining, observing and taking pictures under a microscope, as shown in Figure 2 , and then performing statistical analysis.

[0106] 4.2.4 Test results

[0107] The B16 cell melanin synthesis inhibition rate is shown in Figure 3 , Table 8 and Table 9.

[0108] Table 8 B16 cell melanin synthesis inhibition rate

[0109]

[0110] As can be seen from Figure 2 , Figure 3 and Table 8, compared with the blank control group, the melanin content and melanin synthesis inhibition of B16 cells after treatment with 50 μg / mL of kojic acid (positive control group) were significantly decreased, proving that the experimental system was established.

[0111] Melanin staining showed that the melanin content of B16 cells was significantly reduced after treatment with 0.005%, 0.01%, 0.02%, and 0.03% of Sanmu Core (Example 1);

[0112] The melanin synthesis inhibition rate of B16 cells treated with 0.005%, 0.01%, 0.02%, and 0.03% of Sanmu Core (Example 1) was 65.46%, 75.85%, 76.51%, and 79.98%, respectively, which was significantly different from the blank control group (p<0.01), indicating that Sanmu Core (Example 1) had a significant inhibitory effect on B16 cell melanin synthesis.

[0113] It can also be seen that when the content of the whitening composition provided by Example 1 is 0.03%, the B16 cell melanin synthesis inhibition rate is the highest. Therefore, the B16 cell melanin synthesis inhibition test was performed on the compositions provided by Examples 2-7 and Comparative Examples 1-4 with a volume concentration of 0.03%, and the B16 cell melanin synthesis inhibition rate is shown in Table 9.

[0114] Table 9 Melanin synthesis inhibition rate data table

[0115] Sample Melanin synthesis inhibition rate (%) Example 2 79.98 Example 3 81.64 Example 4 80.93 Example 5 83.15 Example 6 84.23 Example 7 88.36 Comparative Example 1 63.53 Comparative Example 2 66.17 Comparative Example 3 63.96 Comparative Example 4 61.22

[0116] In summary, Sanmu Core has a significant inhibitory effect on B16 cell melanin synthesis.

[0117] Application Example 1

[0118] This example provides a toner consisting of 1 wt% of the whitening composition described in Example 1 and 99 wt% of a conventional base for toner.

[0119] Example 2

[0120] This example provides an emulsion consisting of 6 wt% of the antioxidant repair composition of Example 2 and 94 wt% of an emulsion conventional base.

[0121] Example 3

[0122] This example provides a serum consisting of 20 wt% of the antioxidant repair composition of Example 3 and 80 wt% of a serum conventional base.

[0123] Example 4

[0124] This example provides a mask consisting of 3.5 wt% of the antioxidant repair composition of Example 4 and 96.5 wt% of a mask conventional base.

[0125] It will be apparent to those skilled in the art that the application is not limited to the details of the foregoing exemplary embodiments and that the present application can be implemented in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore considered in all respects to be illustrative and not restrictive, the scope of the application being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. No feature of the application is considered critical unless otherwise indicated in the claims.

Claims

1. A whitening composition, characterized by, The active components of the whitening composition comprise, by mass fraction, 20 parts of coix seed fermentation liquor, 10 parts of peony extract combination factor, 30 parts of 4-butylresorcinol solution, 5 parts of zinc gluconate, 0.75 parts of decarboxylated myo-inositol hydrochloride, 0.75 parts of polygonatum extract combination factor, 21-32.75 parts of water, 4-6 parts of butanediol, 0.4-0.6 parts of 1,2-hexanediol, and 0.4-0.6 parts of p-hydroxyacetophenone; The coix seed fermentation liquor comprises, by mass fraction based on 100% of the total mass, 30wt% of coix seed extract, 68wt% of glycerol, and 2wt% of 1,2-hexanediol; The peony extract combination factor comprises, by mass fraction based on 100% of the total mass, 30wt% of peony root extract, 30wt% of butanediol, 39wt% of water, 0.5wt% of 1,2-hexanediol, and 0.5wt% of p-hydroxyacetophenone; The 4-butylresorcinol solution comprises, by mass fraction based on 100% of the total mass, 10wt% of 4-butylresorcinol, 40wt% of hydroxypropyl cyclodextrin, 44wt% of water, 5wt% of 1,3-propanediol, 0.5wt% of 1,2-hexanediol, and 0.5wt% of p-hydroxyacetophenone; The polygonatum extract combination factor comprises, by mass fraction based on 100% of the total mass, 50wt% of polygonatum cyrtonema rhizome / root extract, 5wt% of butanediol, 5wt% of 1,2-pentanediol, and 40wt% of water.

2. A whitening composition, characterized by, The active components of the whitening composition comprise, by mass fraction, 20 parts of coix seed fermentation liquor, 10 parts of peony extract combination factor, 30 parts of 4-butylresorcinol solution, 5 parts of zinc gluconate, 0.75 parts of decarboxylated myo-inositol hydrochloride, 0.75 parts of polygonatum extract combination factor, 21-32.75 parts of water, 4-6 parts of butanediol, 0.4-0.6 parts of 1,2-hexanediol, and 0.4-0.6 parts of p-hydroxyacetophenone; The coix seed fermentation liquor comprises, by mass fraction based on 100% of the total mass, 30wt% of coix seed extract, 68wt% of glycerol, and 2wt% of 1,2-hexanediol; The peony extract combination factor comprises, by mass fraction based on 100% of the total mass, 30wt% of peony root extract, 30wt% of butanediol, 39wt% of water, 0.5wt% of 1,2-hexanediol, and 0.5wt% of p-hydroxyacetophenone; The 4-butylresorcinol solution comprises, by mass fraction based on 100% of the total mass, 10wt% of 4-butylresorcinol, 40wt% of hydroxypropyl cyclodextrin, 44wt% of water, 5wt% of 1,3-propanediol, 0.5wt% of 1,2-hexanediol, and 0.5wt% of p-hydroxyacetophenone; The polygonatum extract combination factor comprises, by mass fraction based on 100% of the total mass, 50wt% of polygonatum cyrtonema rhizome / root extract, 5wt% of butanediol, 5wt% of 1,2-pentanediol, and 40wt% of water.

3. Use of the whitening composition according to any one of claims 1-2 for the preparation of a cosmetic product.

4. A cosmetic product, characterized by, containing 1 wt% to 20 wt% of the whitening composition according to any one of claims 1-2.

5. The cosmetic product according to claim 4, characterized in that, containing 3.5 wt% to 20 wt% of the whitening composition according to any one of claims 1-2.

6. The cosmetic product according to claim 4, characterized in that, the cosmetic product is one of a cosmetic water, a cosmetic emulsion, a cosmetic cream, a cosmetic serum, a cosmetic mask, and a cosmetic bath. the cosmetic product is one of a cosmetic water, a cosmetic emulsion, a cosmetic cream, a cosmetic serum, a cosmetic mask, and a cosmetic bath.

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