Vitamin-embedded prolamin nanoparticles and preparation method thereof

Abstract

The invention belongs to the technical field of functional materials, and relates to preparation of an active substance delivery system, in particular to a vitamin-embedded prolamin nanoparticle and a preparation method thereof. Dissolving alcohol soluble protein and vitamins by adopting an ethanol alkali solution to obtain an organic phase; the ethanol-alkali solution is an ethanol aqueous solution containing an alkali compound, and the vitamin comprises vitamin D; dissolving sodium casein in water to prepare a water phase; adding the organic phase into the water phase through an anti-solvent method to form sodium casein-coated vitamin-loaded prolamin particles, so as to obtain colloidal dispersion liquid; and removing ethanol in the colloidal dispersion liquid, adjusting the pH value to be neutral, and drying to obtain the product. The alkaline alcohol solution used in the preparation method provided by the invention can influence the embedding process by inducing prolamin to expand, and the provided alkaline environment not only can enhance the stability of vitamin A and vitamin D, but also improves the embedding rate, slows down the release speed and enhances the storage stability.

Description

Technical Field

[0001] This invention belongs to the field of functional materials technology and relates to the preparation of active substance delivery systems, specifically to a vitamin-encapsulating alcohol-soluble protein nanoparticle and its preparation method. Background Technology

[0002] The information disclosed in this background section is intended only to enhance understanding of the overall background of the invention and is not necessarily to be construed as an admission or in any way implying that such information constitutes prior art known to those skilled in the art.

[0003] Vitamins A and D, as fat-soluble vitamins, play important roles in regulating metabolism and maintaining normal physiological functions, especially in the growth and development of epithelial cells, vision, reproductive organs, bones, and teeth in fetuses and infants. However, the low solubility of fat-soluble vitamins and their sensitivity to external air, temperature, light, and the digestive system limits their oral bioavailability.

[0004] To improve the oral bioavailability of vitamins A and D, biocompatible and low-cost alcohol-soluble proteins can be used to encapsulate vitamins A and D. However, during the research process, it was found that the solvents used to dissolve alcohol-soluble proteins are alcohol-based solvents, such as aqueous solutions of ethanol, methanol, and ethylene glycol. These solutions inevitably contain oxygen, and vitamin D is unstable in neutral aqueous solutions. Therefore, during the process of preparing alcohol-soluble protein nanoparticles by encapsulating vitamins A and D with alcohol-soluble proteins, vitamin D is easily destabilized, thereby reducing its bioavailability. Summary of the Invention

[0005] To address the shortcomings of existing technologies, the present invention aims to provide a vitamin-encapsulated prolysin nanoparticle and its preparation method. The preparation method provided by the present invention not only improves the stability of vitamin D during the preparation process, but also enhances the stability of vitamin A and vitamin D after encapsulation, increases the encapsulation rate of vitamin A and vitamin D, and slows down the release rate, thereby improving the oral bioavailability of vitamin A and vitamin D.

[0006] To achieve the above objectives, the technical solution of the present invention is as follows: In a first aspect, a method for preparing vitamin-encapsulated prolysin nanoparticles includes the following steps: An organic phase is obtained by dissolving alcohol-soluble proteins and vitamins in an ethanol-alkali solution; the ethanol-alkali solution is an aqueous solution of ethanol containing an alkaline compound, and the vitamins include vitamin D. Sodium casein was dissolved in water to prepare an aqueous phase; An organic phase is added to an aqueous phase by an antisolvent method to form vitamin-loaded alcohol-soluble protein particles encapsulated by sodium casein, thereby obtaining a colloidal dispersion. After removing the ethanol from the colloidal dispersion, adjust the pH to neutral and dry to obtain the final product.

[0007] To address the instability of vitamin D caused by using alcohol solvents for encapsulating vitamin A and D with alcohol-soluble proteins, this invention adds an alkaline compound to an ethanol-water solution to improve the stability of vitamin D during preparation. Unexpectedly, the addition of the alkaline compound increased the encapsulation rate of vitamin A and D by the alcohol-soluble protein particles. This is because the alkaline ethanol solution can enhance the intermolecular interactions between alcohol-soluble proteins and vitamins A and D by inducing the unfolding of alcohol-soluble proteins to expose their binding sites, ultimately enhancing the solubility and stability of vitamins A and D. Furthermore, the alkaline environment provided by the ethanol solution strengthens the self-assembly forces between alcohol-soluble proteins and vitamins A and D, thereby enhancing the sustained-release effect of nanoparticles on vitamins A and D, reducing the release rate and improving the accessibility of vitamins A and D.

[0008] Although encapsulating vitamins in proteases effectively improves their stability, the overall stability of the nanoparticle dispersion suffers from aggregation and precipitation due to the proximity to the isoelectric point. Furthermore, proteases exhibit poor solubility in aqueous solutions; therefore, if the outer layer of the nanoparticles lacks hydrophilic groups, their solubility in aqueous solutions after freeze-drying is also poor, resulting in limited improvement in vitamin water solubility. Firstly, sodium casein carries a negative charge, providing electrostatic interactions on the outer layer of the nanoparticles to stabilize the entire system. Secondly, sodium casein possesses hydrophobic groups that bind to proteases through intermolecular forces, while its hydrophilic groups, located on the outer layer of the nanoparticles, enable dissolution in aqueous solutions. Therefore, this invention selects sodium casein to improve stability and redispersibility during the preparation of nanoparticles.

[0009] Secondly, a type of alcohol-soluble protein nanoparticle encapsulating vitamins is obtained by the above preparation method.

[0010] Thirdly, the application of the above-mentioned vitamin-encapsulated prolysin nanoparticles in the preparation of vitamin supplement products.

[0011] The product described in this invention is a medicine or health food, and the health food may be a nutritional supplement, etc.

[0012] The beneficial effects of this invention are as follows: This invention utilizes the dissolution of alcohol-soluble proteins and vitamins under alkaline ethanol solution conditions to form intermolecular interactions, increasing solvent polarity and promoting protein polymerization under lower pH conditions. The advantage lies in the fact that alkaline ethanol solution as a solvent can promote the formation of hydrogen bonds and hydrophobic interactions between vitamins and alcohol-soluble proteins, which is more conducive to the encapsulation of vitamins A and D. The alcohol-soluble protein nanoparticles provided by this invention can be used for the protection and delivery of vitamins. Attached Figure Description

[0013] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an improper limitation of the invention.

[0014] Figure 1 The particle size of alcohol-soluble proteins dissolved in ethanol aqueous solutions with different sodium hydroxide concentrations in the preliminary experiment of this invention; Figure 2 Transmission electron microscopy images of the alcohol-soluble protein nanoparticles prepared in Example 6 of this invention, A is low magnification and B is high magnification; Figure 3 The image shows the encapsulation efficiency of vitamin A and vitamin D in the alcohol-soluble protein nanoparticles prepared according to various embodiments of the present invention (hereinafter referred to as examples); Figure 4 The release curves of vitamin D3 from the alcohol-soluble protein nanoparticles prepared in the various embodiments of the present invention (hereinafter referred to as examples) are shown. Figure 5 The retention curves of vitamin D3 in the alcohol-soluble protein nanoparticles prepared in the various embodiments of the present invention (hereinafter referred to as examples) at different times. Detailed Implementation

[0015] It should be noted that the following detailed descriptions are exemplary and intended to provide further illustration of the invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0016] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of exemplary embodiments according to the invention. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0017] In view of the problems of low vitamin D stability and low vitamin D encapsulation rate when using existing methods to encapsulate vitamin D with alcohol-soluble proteins, this invention proposes an alcohol-soluble protein nanoparticle for encapsulating vitamin D and its preparation method.

[0018] A typical embodiment of the present invention provides a method for preparing vitamin-encapsulated alcohol-soluble protein nanoparticles, comprising the following steps: An organic phase is obtained by dissolving alcohol-soluble proteins and vitamins in an ethanol-alkali solution; the ethanol-alkali solution is an aqueous solution of ethanol containing an alkaline compound, and the vitamins include vitamin D. Sodium casein was dissolved in water to prepare an aqueous phase; An organic phase is added to an aqueous phase by an antisolvent method to form vitamin-loaded alcohol-soluble protein particles encapsulated by sodium casein, thereby obtaining a colloidal dispersion. After removing the ethanol from the colloidal dispersion, adjust the pH to neutral and dry to obtain the final product.

[0019] This invention uses an alkaline ethanol solution as a solvent. This solvent can provide charge, causing the amino or carboxyl groups on the surface of protein molecules to become charged, generating mutual repulsion between charges, which leads to the depolymerization and unfolding of protein molecules, thereby enhancing the stability and solubility of alcohol-soluble proteins. At the same time, in an alkaline environment, the carboxyl and amino groups of alcohol-soluble proteins form hydrogen bonds and hydrophobic interactions with the carbonyl groups of vitamins, and these hydrogen bonds and hydrophobic interactions promote the loading of vitamins into the particles. Finally, by adding substances to adjust the pH to neutral, protons are provided, reducing the mutual repulsion between molecules and guiding the formation of nanoparticles, so as to achieve pH-induced self-assembly and the relative inner layer of particles that can load vitamins.

[0020] In some embodiments, the vitamin further includes vitamin A. Specifically, the vitamin A is one or more of retinol, retinaldehyde, retinoic acid, retinyl acetate (vitamin A acetate), retinyl palmitate, etc.

[0021] In some embodiments, the ethanol-based solution is prepared by mixing ethanol with an aqueous sodium hydroxide solution at a concentration of 20–200 mmol / L. Specifically, the concentration of the aqueous sodium hydroxide solution is 110–130 mmol / L. Studies have shown that the ethanol-based solution at this concentration has a better encapsulation effect on vitamins. Specifically, the volume fraction of the ethanol-based solution is 75–85%. Studies have shown that this condition has a better dissolution effect on alcohol-soluble proteins and vitamins, thereby increasing the encapsulation effect of alcohol-soluble proteins.

[0022] In some embodiments, the mass ratio of vitamins to prolamins is 0.1–1.0:2–6. Studies have shown that this condition improves vitamin bioavailability and avoids waste.

[0023] In some embodiments, the concentration of prolactin in the organic phase is 2-6% (w / v). Under these conditions, the prolactin disperses better, resulting in better encapsulation.

[0024] In some embodiments, the concentration of sodium casein in the aqueous phase is 0.3-1% (w / v).

[0025] In some embodiments, the volume ratio of the organic phase to the aqueous phase is 1:5 to 20. This condition is conducive to the formation of vitamin-loaded prolysin particles encapsulated in sodium casein.

[0026] In some embodiments, rotary evaporation is used to remove ethanol from the colloidal dispersion.

[0027] In some embodiments, the pH is adjusted to neutral using a citric acid solution. Specifically, the concentration of the citric acid solution is 0.3–0.7 mmol / L.

[0028] In some embodiments, the drying is freeze-drying. Freeze-drying can better protect the structure of the prolysin nanoparticles encapsulating vitamins.

[0029] Another embodiment of the present invention provides an alcohol-soluble protein nanoparticle encapsulating vitamins, obtained by the above preparation method.

[0030] A third embodiment of the present invention provides the application of the above-mentioned vitamin-encapsulated prolysin nanoparticles in the preparation of vitamin supplement products.

[0031] To enable those skilled in the art to better understand the technical solution of the present invention, the technical solution of the present invention will be described in detail below with reference to specific embodiments.

[0032] Preliminary experiment An ethanol-based alkaline solution was prepared by mixing sodium hydroxide solutions with concentrations of 0, 30, 60, 90, 120, 150, and 210 mmol / L with ethanol at a volume ratio of 20:80. This alkaline solution was then used to dissolve alcohol-soluble protein. The ethanol-based alkaline solution of alcohol-soluble protein was added to an aqueous phase containing dissolved sodium casein. After removing the ethanol by rotary evaporation, the pH of the system was adjusted to neutral using citric acid. The average particle size (as particle size) of the unloaded alcohol-soluble protein-sodium casein nanoparticles (with an alcohol-soluble protein concentration of 45 mg / mL in the solution) was determined using dynamic light scattering (DLS). The results are as follows: Figure 1 As shown, the particle size of alcohol-soluble protein nanoparticles first decreases and then increases with increasing sodium hydroxide solution concentration, indicating that an alkaline environment facilitates the dissolution of alcohol-soluble proteins and the formation of nanoparticles. The particle size subsequently increases due to the increased ionic strength in the solution. Smaller particle sizes are more easily taken up by cells and cross the cell membrane, thus enhancing their biological activity. Figure 1 The results show that the nanoparticles have the smallest particle size when the sodium hydroxide solution concentration is 120 mmol / L in an ethanol alkaline solution, making them more suitable as a solvent for alcohol-soluble proteins.

[0033] Example 1 A method for preparing an alcohol-soluble protein nanoparticle encapsulating vitamin A and vitamin D includes the following steps: 1. Mix ethanol and pure water at a volume ratio of 80:20 to prepare an ethanol aqueous solution with a volume concentration of 80%.

[0034] 2. Disperse the alcohol-soluble protein, vitamin A acetate, and vitamin D3 in the ethanol-water solution prepared in step 1, stir to dissolve, and let stand for 4 h to obtain the organic phase. The concentrations of alcohol-soluble protein, vitamin A acetate, and vitamin D3 are 45 mg / mL, 4.5 mg / mL, and 1.5 mg / mL, respectively.

[0035] 3. Dissolve sodium casein in water and stir overnight to fully hydrate it, so as to prepare an aqueous phase of sodium casein with a concentration of 10 mg / mL.

[0036] 4. Add 4 mL of the organic phase prepared in step 2 dropwise to 20 mL of the sodium casein aqueous phase prepared in step 3 at 800 rpm, stir for 5 minutes, remove ethanol by rotary evaporation and freeze dry to obtain alcohol-soluble protein particles loaded with vitamin A acetate and vitamin D3 encapsulated by sodium casein, which are alcohol-soluble protein nanoparticles encapsulated with vitamin A and vitamin D.

[0037] Example 2 A method for preparing an alcohol-soluble protein nanoparticle encapsulating vitamin A and vitamin D includes the following steps: 1. Prepare an 80% ethanol-alkali solution by mixing ethanol and a 60 mmol / L sodium hydroxide solution at a volume ratio of 80:20.

[0038] 2. Disperse the alcohol-soluble protein, vitamin A acetate, and vitamin D3 in the ethanol-alkali solution prepared in step 1, stir to dissolve, and let stand for 4 hours. The concentrations of alcohol-soluble protein, vitamin A acetate, and vitamin D3 are 45 mg / mL, 4.5 mg / mL, and 1.5 mg / mL, respectively.

[0039] 3. Dissolve sodium casein in water and stir overnight to fully hydrate it, obtaining an aqueous phase of sodium casein with a concentration of 10 mg / mL.

[0040] 4. Add 4 mL of the organic phase prepared in step 2 dropwise to 20 mL of the sodium casein aqueous phase prepared in step 3 at 800 rpm, stir for 5 minutes, remove ethanol by rotary evaporation, and then slowly add 0.5 mmol / L citric acid solution to neutralize the pH to 7. After freeze-drying, obtain the alcohol-soluble protein particles loaded with vitamin A acetate and vitamin D3 encapsulated by sodium casein, which are the alcohol-soluble protein nanoparticles encapsulated with vitamin A and vitamin D.

[0041] Example 3 A method for preparing an alcohol-soluble protein nanoparticle encapsulating vitamin A and vitamin D includes the following steps: 1. Prepare an 80% ethanol-alkali solution by mixing ethanol and a 120 mmol / L sodium hydroxide solution at a volume ratio of 80:20.

[0042] 2. Disperse the alcohol-soluble protein, vitamin A acetate, and vitamin D3 in the ethanol-alkali solution prepared in step 1, stir to dissolve, and let stand for 4 hours. The concentrations of alcohol-soluble protein, vitamin A acetate, and vitamin D3 are 45 mg / mL, 4.5 mg / mL, and 1.5 mg / mL, respectively.

[0043] 3. Dissolve sodium casein in water and stir overnight to fully hydrate it, obtaining an aqueous phase of sodium casein with a concentration of 10 mg / mL.

[0044] 4. Add 4 mL of the organic phase prepared in step 2 dropwise to 20 mL of the sodium casein aqueous phase prepared in step 3 at 800 rpm, stir for 5 minutes, remove ethanol by rotary evaporation, and then slowly add 0.5 mmol / L citric acid solution to neutralize the pH to 7. After freeze-drying, obtain the alcohol-soluble protein particles loaded with vitamin A acetate and vitamin D3 encapsulated by sodium casein, which are the alcohol-soluble protein nanoparticles encapsulated with vitamin A and vitamin D.

[0045] Example 4 A method for preparing an alcohol-soluble protein nanoparticle encapsulating vitamin A and vitamin D includes the following steps: 1. Mix ethanol and pure water at a volume ratio of 80:20 to prepare an ethanol aqueous solution with a volume concentration of 80%.

[0046] 2. Disperse the alcohol-soluble protein, vitamin A acetate, and vitamin D3 in the ethanol-water solution prepared in step 1, stir to dissolve, and let stand for 4 h to obtain the organic phase. The concentrations of alcohol-soluble protein, vitamin A acetate, and vitamin D3 are 45 mg / mL, 9 mg / mL, and 3 mg / mL, respectively.

[0047] 3. Dissolve sodium casein in water and stir overnight to fully hydrate it, obtaining an aqueous phase of sodium casein with a concentration of 10 mg / mL.

[0048] 4. Add 4 mL of the organic phase prepared in step 2 dropwise to 20 mL of the sodium casein aqueous phase prepared in step 3 at 800 rpm, stir for 5 minutes, remove ethanol by rotary evaporation and freeze dry to obtain alcohol-soluble protein particles loaded with vitamin A acetate and vitamin D3 encapsulated by sodium casein, which are alcohol-soluble protein nanoparticles encapsulated with vitamin A and vitamin D.

[0049] Example 5 A method for preparing an alcohol-soluble protein nanoparticle encapsulating vitamin A and vitamin D includes the following steps: 1. Prepare an 80% ethanol-alkali solution by mixing ethanol and a 60 mmol / L sodium hydroxide solution at a volume ratio of 80:20.

[0050] 2. Disperse the alcohol-soluble protein, vitamin A acetate, and vitamin D3 in the ethanol-alkali solution prepared in step 1, stir to dissolve, and let stand for 4 hours. The concentrations of alcohol-soluble protein, vitamin A acetate, and vitamin D3 are 45 mg / mL, 9 mg / mL, and 3 mg / mL, respectively.

[0051] 3. Dissolve sodium casein in water and stir overnight to fully hydrate it, obtaining an aqueous phase of sodium casein with a concentration of 10 mg / mL.

[0052] 4. Add 4 mL of the organic phase prepared in step 2 dropwise to 20 mL of the sodium casein aqueous phase prepared in step 3 at 800 rpm, stir for 5 minutes, remove ethanol by rotary evaporation, and then slowly add 0.5 mmol / L citric acid solution to neutralize the pH to 7. After freeze-drying, obtain the alcohol-soluble protein particles loaded with vitamin A acetate and vitamin D3 encapsulated by sodium casein, which are the alcohol-soluble protein nanoparticles encapsulated with vitamin A and vitamin D.

[0053] Example 6 A method for preparing an alcohol-soluble protein nanoparticle encapsulating vitamin A and vitamin D includes the following steps: 1. Prepare an 80% ethanol-alkali solution by mixing ethanol and a 120 mmol / L sodium hydroxide solution at a volume ratio of 80:20.

[0054] 2. Disperse the alcohol-soluble protein, vitamin A acetate, and vitamin D3 in the ethanol-alkali solution prepared in step 1, stir to dissolve, and let stand for 4 hours. The concentrations of alcohol-soluble protein, vitamin A acetate, and vitamin D3 are 45 mg / mL, 9 mg / mL, and 3 mg / mL, respectively.

[0055] 3. Dissolve sodium casein in water and stir overnight to fully hydrate it, obtaining an aqueous phase of sodium casein with a concentration of 10 mg / mL.

[0056] 4. Add 4 mL of the organic phase prepared in step 2 dropwise to 20 mL of the sodium casein aqueous phase prepared in step 3 at 800 rpm, stir for 5 minutes, remove ethanol by rotary evaporation, and then slowly add 0.5 mmol / L citric acid solution to neutralize the pH to 7. After freeze-drying, obtain the alcohol-soluble protein particles loaded with vitamin A acetate and vitamin D3 encapsulated by sodium casein, which are the alcohol-soluble protein nanoparticles encapsulated with vitamin A and vitamin D.

[0057] The nanoparticles prepared in this embodiment have a smooth spherical shell-core structure, such as... Figure 2 As shown.

[0058] Example 7 A method for preparing an alcohol-soluble protein nanoparticle encapsulating vitamin D includes the following steps: 1. Mix ethanol and pure water at a volume ratio of 80:20 to prepare an ethanol aqueous solution with a volume concentration of 80%.

[0059] 2. Disperse the alcohol-soluble protein and vitamin D3 in the ethanol-water solution prepared in step 1, stir to dissolve, and let stand for 4 h to obtain the organic phase. The concentrations of alcohol-soluble protein and vitamin D3 are 45 mg / mL and 3 mg / mL, respectively.

[0060] 3. Dissolve sodium casein in water and stir overnight to fully hydrate it, obtaining an aqueous phase of sodium casein with a concentration of 10 mg / mL.

[0061] 4. Add 4 mL of the organic phase prepared in step 2 dropwise to 20 mL of the sodium casein aqueous phase prepared in step 3 at 800 rpm, stir for 5 minutes, remove ethanol by rotary evaporation and freeze dry to obtain the vitamin D3-loaded alcohol-soluble protein particles encapsulated in sodium casein, which are the vitamin D-encapsulated alcohol-soluble protein nanoparticles.

[0062] Example 8 A method for preparing an alcohol-soluble protein nanoparticle encapsulating vitamin D includes the following steps: 1. Prepare an 80% ethanol-alkali solution by mixing ethanol and a 60 mmol / L sodium hydroxide solution at a volume ratio of 80:20.

[0063] 2. Disperse the alcohol-soluble protein and vitamin D3 in the ethanol-based alkaline solution prepared in step 1, stir to dissolve, and let stand for 4 h to obtain the organic phase. The concentrations of alcohol-soluble protein and vitamin D3 are 45 mg / mL and 3 mg / mL, respectively.

[0064] 3. Dissolve sodium casein in water and stir overnight to fully hydrate it, obtaining an aqueous phase of sodium casein with a concentration of 10 mg / mL.

[0065] 4. Add 4 mL of the organic phase prepared in step 2 dropwise to 20 mL of the sodium casein aqueous phase prepared in step 3 at 800 rpm, stir for 5 minutes, remove ethanol by rotary evaporation, and then slowly add 0.5 mmol / L citric acid solution to neutralize the pH to 7. After freeze-drying, obtain the vitamin D3-loaded prolysin particles encapsulated in sodium casein, which are the vitamin D-encapsulated prolysin nanoparticles.

[0066] In the above embodiments, Examples 1, 4, and 7 used an aqueous ethanol solution as a solvent to prepare vitamin-loaded nanoparticles; Examples 2, 5, and 8 used a 60 mmol / L sodium hydroxide solution in an ethanol-based alkaline solution as a solvent to prepare vitamin-loaded nanoparticles; and Examples 3 and 6 used a 120 mmol / L sodium hydroxide solution in an ethanol-based alkaline solution as a solvent to prepare vitamin-loaded nanoparticles. The encapsulation efficiency and vitamin retention rate were measured.

[0067] The encapsulation efficiency was determined by the ratio of the active material loaded into the nanoparticles to the total amount of active material added. The loaded active material was dissolved in the supernatant using dimethyl sulfoxide and then analyzed by HPLC.

[0068]

[0069] Storage stability testing involves placing the colloidal dispersion carrying the active substance at 4°C for one month and then measuring the concentration of the active substance in the supernatant. The storage retention rate of the active substance is obtained by comparing the concentration of the active substance after one month of storage with the initial (freshly prepared) concentration.

[0070]

[0071] Figure 3 This indicates that ethanol-based alkaline solutions, when used as solvents, can enhance the intermolecular interactions between alcohol-soluble proteins and vitamins, thereby increasing the vitamin encapsulation rate.

[0072] Figure 4 This indicates that the ethanol alkaline solution can effectively and slowly release vitamins.

[0073] Figure 5 This indicates that ethanol alkaline solution can improve the storage stability of vitamins.

[0074] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A method for preparing vitamin-encapsulated alcohol-soluble protein nanoparticles, characterized in that, Includes the following steps: An organic phase is obtained by dissolving alcohol-soluble proteins and vitamins in an ethanol-alkali solution; the ethanol-alkali solution is an aqueous solution of ethanol containing an alkaline compound, and the vitamins include vitamin D. Sodium casein was dissolved in water to prepare an aqueous phase; An organic phase is added to an aqueous phase by an antisolvent method to form vitamin-loaded alcohol-soluble protein particles encapsulated by sodium casein, thereby obtaining a colloidal dispersion. After removing the ethanol from the colloidal dispersion, adjust the pH to neutral and dry to obtain the final product.

2. The preparation method according to claim 1, characterized in that, The vitamins mentioned also include vitamin A.

3. The preparation method according to claim 1, characterized in that, The ethanol alkaline solution is prepared by mixing ethanol with an aqueous solution of sodium hydroxide, the concentration of which is 20-200 mmol / L.

4. The preparation method according to claim 3, characterized in that sodium hydroxide... The concentration of the aqueous solution is 110~130 mmol / L; Alternatively, the volume fraction of the ethanol-based alkaline solution may be 75-85%.

5. The preparation method according to claim 1, characterized in that, The mass ratio of vitamins to prolamins is 0.1~1.0:2~6.

6. The preparation method according to claim 1, characterized in that, The concentration of alcohol-soluble proteins in the organic phase is 2-6%, w / v; Alternatively, the concentration of sodium casein in the aqueous phase is 0.3-1%, w / v.

7. The preparation method according to claim 1, characterized in that: The volume ratio of the organic phase to the aqueous phase is 1:5~20.

8. The preparation method according to claim 1, characterized in that: Rotary evaporation was used to remove ethanol from the colloidal dispersion. Alternatively, adjust the pH to neutral using a citric acid solution; Alternatively, the drying process may be freeze-drying.

9. A type of alcohol-soluble protein nanoparticle encapsulating vitamins, characterized in that, Obtained by the preparation method described in any one of claims 1 to 8.

10. The use of the vitamin-encapsulated prolysin nanoparticles of claim 9 in the preparation of vitamin supplement products.

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