Traditional Chinese medicine exosome composition with hair-growing and hair-loss-preventing functions and hair-growing and hair-loss-preventing essence prepared from traditional Chinese medicine exosome composition

Abstract

The invention belongs to the technical field of traditional Chinese medicine composite exosomes, and particularly relates to a traditional Chinese medicine exosome composition with hair-growing and hair-loss-preventing functions and hair-growing and hair-loss-preventing essence prepared from the traditional Chinese medicine exosome composition. The composition is prepared from the following raw materials in parts by weight: 5 to 10 parts of resveratrol exosome, 5 to 10 parts of baicalein exosome, 5 to 10 parts of radix polygoni multiflori exosome, 5 to 10 parts of radix angelicae sinensis exosome, 8 to 15 parts of humectant, 0.5 to 1.5 parts of solubilizer, 5 to 10 parts of skin conditioner, 1 to 5 parts of surfactant and 2 to 8 parts of grease. On the basis of the traditional Chinese medicine synergistic hair-growing theory, resveratrol, baicalein, polygonum multiflorum and angelica sinensis exosomes are systematically integrated as main functional components for the first time, a synergistic anti-hair-loss hair-growing essence system is constructed, and the whole-process intervention from anti-inflammation and anti-oxidation to activation of hair follicle regeneration and improvement of scalp microenvironment is achieved; and a more efficient, safer and more targeted technical path is provided for hair growth and hair loss prevention.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine compound exosome technology, and particularly relates to a traditional Chinese medicine exosome composition with hair growth and hair loss prevention function and a hair growth and hair loss prevention essence prepared therefrom. Background Technology

[0002] Hair loss and thinning hair have become increasingly prevalent globally in recent years, impacting self-confidence and mental health. Major contributing factors include genetics, hormonal imbalances, stress, environmental pollution, and nutritional deficiencies. While traditional drug treatments (such as minoxidil and finasteride) can slow the progression of hair loss to some extent, they are often accompanied by side effects, high dependence, and slow effectiveness, limiting their long-term use.

[0003] In contrast, hair growth products based on natural plant extracts have gained attention due to their safety and good tolerability. Traditional Chinese medicine ingredients such as Scutellaria baicalensis, Polygonum multiflorum, and Angelica sinensis have been proven to have multiple hair growth effects, including anti-inflammatory, antioxidant, and promotion of hair follicle microcirculation and melanin synthesis. However, current plant extract products generally suffer from low bioavailability of active ingredients, poor transdermal absorption, and weak targeting, resulting in limited hair growth effects.

[0004] In recent years, exosomes, as an emerging natural nanocarrier, have shown great potential in drug delivery and functional activity release due to their excellent cell affinity and targeted delivery capabilities, becoming a research hotspot for the carrier of plant functional components. However, problems such as simple formulation structure, lack of functional dimensions, low delivery efficiency, and unclear synergistic mechanisms among plant components still exist, limiting their widespread application in clinical practice or the market.

[0005] In view of this, this invention, based on the theory of synergistic hair growth in traditional Chinese medicine, systematically integrates resveratrol, baicalin, Polygonum multiflorum and Angelica sinensis exosomes as the main functional ingredients for the first time, and constructs a synergistic hair loss prevention and hair growth essence system. It aims to achieve full-process intervention from anti-inflammatory and antioxidant to activating hair follicle regeneration and improving the scalp microenvironment, and provide a more efficient, safer and more targeted technical path for hair growth and hair loss prevention. Summary of the Invention

[0006] The purpose of this invention is to provide a Chinese herbal exosome composition with hair growth and anti-hair loss functions, and a hair growth and anti-hair loss essence prepared therefrom.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows: A traditional Chinese medicine exosome composition with hair growth and anti-hair loss function is composed of the following raw materials in parts by weight: 5-10 parts of resveratrol exosomes, 5-10 parts of baicalin exosomes, 5-10 parts of Polygonum multiflorum exosomes, 5-10 parts of Angelica sinensis exosomes, 8-15 parts of moisturizer, 0.5-1.5 parts of solubilizer, 5-10 parts of skin conditioning agent, 1-5 parts of surfactant, and 2-8 parts of oil.

[0008] Furthermore, the preparation methods for resveratrol exosomes, baicalin exosomes, Polygonum multiflorum exosomes, and Angelica sinensis exosomes are as follows: grape skins, Scutellaria baicalensis roots, processed Polygonum multiflorum tuberous roots, and Angelica sinensis rhizomes are selected as raw materials, respectively, and chopped under aseptic conditions to prepare callus tissue. These are inoculated into MS medium and cultured in the dark at 25°C for 2 weeks. After callus formation, they are transferred to a liquid medium containing 3% sucrose, 0.5 mg / L kinetin, and 0.1 mg / L auxin, and cultured in a shaker for 7 days to induce exosome secretion. Then, the plant cell culture supernatant is collected and centrifuged at 300×g for 10 min to remove impurities; then centrifuged at 10,000×g for 30 min to remove large particles; finally, the exosomes are precipitated by ultracentrifugation at 100,000×g for 90 min. The precipitate is resuspended in PBS and sterilized through a 0.22 μm filter membrane, and then stored at 4°C for later use.

[0009] Furthermore, the moisturizer is glycerin or sodium hyaluronate, the solubilizer is octyl glycol, the skin conditioning agent is biotin, the surfactant is PEG-40 hydrogenated castor oil, and the oil is olive oil.

[0010] Another object of the present invention is a hair growth and anti-hair loss serum prepared using the above composition, the serum being prepared by the following steps: S1. Preparation of serum matrix solution: Take 5.0% glycerin, 0.2% sodium hyaluronate, 0.1% biotin, 2.0% PEG-40 hydrogenated castor oil as surfactant, 3.0% olive oil as oil component, and 1.0% octyl glycol as solubilizer, and then add purified water to make up to 100%. Add the above components to a mixing tank in sequence and stir at room temperature for 30 min to obtain the serum matrix solution. S2. Mix resveratrol exosomes, baicalin exosomes, Polygonum multiflorum exosomes, and Angelica sinensis exosomes in a ratio of 1:1:1:1. Add the total exosomes to the essence matrix solution at a ratio of 4% of the total volume of the essence. Stir at 4°C for 30 min to fully disperse and stabilize the exosomes to obtain the pretreated essence. S3. Adjust the H value of the pretreated essence to 5.8–6.3, sterilize it using a 0.22μm filter membrane, fill it into a clean, sterile brown glass bottle, seal it, and store it in a cool, dark place.

[0011] Mechanism of this invention: Resveratrol exosomes can scavenge free radicals and reactive oxygen species, reduce oxidative damage to hair follicles, decrease the expression of inflammatory factors, improve the microenvironment of hair follicles, and slow down hair follicle apoptosis caused by inflammation.

[0012] Baicalein exosomes: inhibit 5-LOX and COX2 activity, effectively relieving inflammation around hair follicles; inhibit androgen-related pathways, reduce excessive sebum secretion, and reduce hair loss caused by sebum clogging hair follicles; enhance dermal papillary cell activity, promote collagen and elastin production, and help repair the scalp barrier.

[0013] Polygonum multiflorum exosomes can directly or indirectly upregulate the expression of key melanin synthases such as tyrosinase and tyrosinase-related protein-1, improving gray hair and hypopigmentation-type hair loss; by dilating capillaries and improving microcirculation, they provide sufficient nutrition and oxygen to hair follicles, which helps the healthy development of new hair; they promote the entry of dermal papilla cells into the S phase, prolonging the hair growth phase and shortening the resting phase.

[0014] Angelica exosomes: promote fibroblast proliferation, enhance the regeneration of the matrix around hair follicles, and help form new hair.

[0015] The advantages of this invention are as follows: This invention provides a traditional Chinese medicine exosome-based anti-hair loss and hair growth essence, which integrates multiple functional plant exosomes such as resveratrol exosomes, baicalin exosomes, Polygonum multiflorum exosomes, and Angelica sinensis exosomes. Active nanoparticles are obtained through natural cell secretion pathways. It is rich in plant-native antioxidant, anti-inflammatory, lipid-regulating, blood circulation-promoting, and hair follicle-activating components, achieving a multi-target, multi-pathway synergistic effect to promote hair growth. It comprehensively improves the scalp microenvironment, enhances hair follicle activity, prolongs the hair growth phase, and significantly alleviates common hair loss problems such as seborrheic and telogen phases. The entire preparation process is gentle and simple, with high activity stability. The product has good biocompatibility and excellent safety, making it suitable for long-term use by people with different types of hair loss. Detailed Implementation

[0016] Example 1 A traditional Chinese medicine exosome composition with hair growth and anti-hair loss function is composed of the following raw materials in parts by weight: 5-10 parts of resveratrol exosomes, 5-10 parts of baicalin exosomes, 5-10 parts of Polygonum multiflorum exosomes, 5-10 parts of Angelica sinensis exosomes, 8-15 parts of moisturizer, 0.5-1.5 parts of solubilizer, 5-10 parts of skin conditioning agent, 1-5 parts of surfactant, and 2-8 parts of oil.

[0017] Preparation of exosomes: Grape skins (resveratrol source), Scutellaria baicalensis root, processed Polygonum multiflorum tuberous root, and Angelica sinensis rhizome were selected as raw materials, and were aseptically chopped and prepared into callus tissues. These were inoculated into MS medium and cultured in the dark at 25°C for 2 weeks. After callus formation, the tissues were transferred to liquid medium containing 3% sucrose, 0.5 mg / L kinetin, and 0.1 mg / L auxin, and cultured on a shaker (80 rpm) for 7 days to induce exosome secretion. The plant cell culture supernatant was collected and centrifuged at 300×g for 10 min to remove impurities; then centrifuged at 10,000×g for 30 min to remove large particles; finally, the exosomes were precipitated by ultracentrifugation at 100,000×g for 90 min. The precipitate was resuspended in PBS, sterilized through a 0.22 μm filter, and stored at 4°C for later use.

[0018] Example 2 Preparation of hair growth and anti-hair loss serum: (1) Prepare the serum base by taking the following ingredients in the following mass percentages: 5.0% glycerin, 0.2% sodium hyaluronate, 0.1% biotin, 1.0% octyl ethylene glycol, and purified water to make up to 100%. Add the above ingredients to the mixing tank in sequence and stir for 30 min at room temperature to make each ingredient fully dissolved and uniform, forming the serum base solution.

[0019] (2) Mix the above exosome solution in a ratio of 1:1:1:1, and the total amount of exosomes added is 4% of the total volume of the essence. Slowly add it to the essence base and stir slowly at a low temperature (4°C) for 30 min to avoid exosome rupture or aggregation.

[0020] (3) Adjust the pH value to 5.8–6.3, use a 0.22 μm filter membrane for sterilization filtration, and finally fill into clean, sterile brown glass bottles, seal and store in the dark and refrigerate. The finished product has good stability and no obvious precipitation or stratification can be observed during storage for more than 12 months.

[0021] Performance testing Experiment 1: Skin Irritation Test (1) Twelve healthy hairless mice were randomly divided into an experimental group and a control group, with six mice in each group.

[0022] (2) The experimental group was treated with 0.2 mL of the essence of this invention on the back skin of mice every day, while the control group was treated with an equal amount of physiological saline for 7 consecutive days.

[0023] (3) Observe and record local skin reactions (erythema, swelling, damage, etc.) daily.

[0024] (4) No obvious erythema, edema or ulceration was observed on the skin of mice in both the experimental group and the control group, indicating that the essence has very low skin irritation.

[0025] Experiment 2: Assay for the Proliferative Activity of Hair Papillary Cells (1) Human dermal papilla cells (hDPCs) were routinely cultured in DMEM / F12 medium to the logarithmic phase.

[0026] (2) Set up a control group (no treatment), a positive control group (minoxidil 50 μM), and a treatment group of this product (1%, 2%, 4% concentration gradient treatment).

[0027] (3) The results of the metabolic activity of each group of cells after 48 hours by the MTT assay are shown in Table 1.

[0028] (4) The essence of the present invention significantly promotes the proliferation of hDPCs cells at a concentration of 1% or higher, and has a good effect on enhancing hair follicle activity.

[0029] Table 1 Experiment 3: Experiment to promote the proliferation of mouse hair follicle cells (1) Take hair follicle tissue from the back of 6-week-old C57BL / 6 mice and isolate primary hair follicle epithelial cells for in vitro culture.

[0030] (2) Experimental groups: blank control group, positive control group (minoxidil 50 μM), low concentration group (1%), medium concentration group (2%) and high concentration group (4%) of this essence.

[0031] (3) Detection method: Cells were seeded in 96-well plates with 5000 cells per well. After treatment for 48 hours, the proliferation capacity was detected by CCK-8 assay and the absorbance was measured at 450 nm. The results are shown in Table 2.

[0032] (4) The results showed that the herbal exosome essence of the present invention could significantly promote the proliferation of mouse hair follicle cells under different concentration conditions, and the concentration-dependent enhancement trend was shown.

[0033] Table 2 Experiment 4: Verification Experiment on the Effect of Anti-Hair Loss and Hair Growth on Volunteers (1) Subjects: A total of 30 volunteers aged 18-45 with mild to moderate hair loss were recruited and randomly divided into the serum treatment group (20 people) and the placebo control group (10 people).

[0034] (2) Trial period: Use once in the morning and once in the evening for 90 consecutive days; take photos of the hair loss area and measure the hair density.

[0035] (3) Detection indicators: The number and density of hair in a specific scalp area are counted using a hair camera, combined with subjective satisfaction scores.

[0036] (4) The test results are shown in Table 3.

[0037] (5) The results showed that after using the essence of the present invention for 90 days, the volunteers’ hair density was significantly increased, their subjective satisfaction was significantly improved, and their hair loss was significantly improved, which was better than that of the control group.

[0038] Table 3 In summary, the present invention provides a traditional Chinese medicine exosome-based hair loss prevention and treatment essence, mainly composed of resveratrol exosomes, baicalein exosomes, Polygonum multiflorum exosomes, and Angelica sinensis exosomes, and formulated with excipients such as glycerin, sodium hyaluronate, propylene glycol, PEG-40 hydrogenated castor oil, oligopeptide-1, biotin, vitamin E, β-glucan, and purified water. This essence is gentle on the skin, rich in various herbal active exosome components, and can promote hair growth, improve the hair follicle microenvironment, and delay the hair loss process through a multi-target mechanism.

Claims

1. A traditional Chinese medicine exosome composition with hair growth and anti-hair loss functions, characterized in that, It is composed of the following raw materials in parts by weight: 5-10 parts resveratrol exosomes, 5-10 parts baicalin exosomes, 5-10 parts Polygonum multiflorum exosomes, 5-10 parts Angelica sinensis exosomes, 8-15 parts moisturizer, 0.5-1.5 parts solubilizer, 5-10 parts skin conditioning agent, 1-5 parts surfactant, and 2-8 parts oil.

2. The herbal exosome composition with hair growth and anti-hair loss function as described in claim 1, characterized in that: The preparation methods for resveratrol exosomes, baicalin exosomes, Polygonum multiflorum exosomes, and Angelica sinensis exosomes are as follows: Grape skins, Scutellaria baicalensis roots, processed Polygonum multiflorum tuberous roots, and Angelica sinensis rhizomes are selected as raw materials, respectively, and chopped and prepared into callus tissue under sterile conditions. The callus tissue is inoculated into MS medium and cultured in the dark at 25°C for 2 weeks. After the callus tissue has formed, it is transferred to a liquid medium containing 3% sucrose, 0.5 mg / L kinetin, and 0.1 mg / L auxin, and cultured in a shaker for 7 days to induce the secretion of exosomes. Then, the supernatant of plant cell culture is collected and centrifuged at 300×g for 10 min to remove impurities. Then, it is centrifuged at 10,000×g for 30 min to remove large particles. Finally, the exosomes are precipitated by ultracentrifugation at 100,000×g for 90 min. The precipitate is resuspended in PBS and sterilized through a 0.22 μm filter membrane. Then, it is stored at 4°C for later use.

3. The herbal exosome composition with hair growth and anti-hair loss function as described in claim 1, characterized in that: The moisturizer is glycerin or sodium hyaluronate, the solubilizer is octyl ethylene glycol, the skin conditioning agent is biotin, the surfactant is PEG-40 hydrogenated castor oil, and the oil is olive oil.

4. A hair growth and anti-hair loss essence prepared using the composition according to any one of claims 1-3, characterized in that, The serum is prepared by the following steps: S1. Preparation of serum matrix solution: Take 5.0% glycerin, 0.2% sodium hyaluronate, 0.1% biotin, 2.0% PEG-40 hydrogenated castor oil, 3.0% olive oil, and 1.0% octyl glycol, and then add purified water to make up to 100%. Add the above ingredients to a mixing tank in sequence and stir at room temperature for 30 minutes to obtain the solution. S2. Mix resveratrol exosomes, baicalin exosomes, Polygonum multiflorum exosomes, and Angelica sinensis exosomes in a ratio of 1:1:1:

1. Add the mixture to the essence matrix solution at a total exosome addition amount of 4% of the total volume of the essence. Stir at 4°C for 30 min to obtain the pretreated essence. S3. Adjust the pH of the pretreated essence to 5.8–6.3, sterilize it using a 0.22μm filter membrane, fill it into a clean, sterile brown glass bottle, seal it, and store it in a cool, dark place.

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