Rat brain embedding box for neuroscience research and low-temperature storage device thereof

Abstract

The invention belongs to the technical field of neuroscience research, and particularly relates to a mouse brain embedding box for neuroscience research and a low-temperature storage device thereof.The mouse brain embedding box comprises a storage cabinet, a separation assembly arranged in the storage cabinet, a refrigeration assembly arranged in the storage cabinet, and a plurality of placement assemblies arranged in the storage cabinet; the multiple placement assemblies are located at the tops of the multiple second partition plates correspondingly, and multiple gas storage assemblies are arranged in the storage cabinet. According to the invention, the embedding box is independently stored in the storage drawer, overall exposure is avoided when the single embedding box is stored and taken, cold air leakage is effectively reduced, cold air is stored in the air storage box through the air storage assembly when the storage drawer is pulled out, and cold air in the air storage box is sent back to the storage drawer through the air storage assembly when the storage drawer retracts; cold air can be recycled, and the effect of reducing cold air waste is achieved; the problem that unnecessary energy waste is likely to be caused in the process of taking the embedding box of an existing refrigeration device is solved.

Description

Technical Field

[0001] This invention relates to the field of neuroscience research technology, and in particular to a mouse brain embedding cassette and its cryogenic storage device for neuroscience research. Background Technology

[0002] In neuroscience research, particularly in animal-model-based brain science, rats are important experimental subjects due to the similarities between their nervous systems and those of humans. Freezing and sectioning rat brain tissue is a crucial preliminary step for subsequent studies such as morphological observation, immunohistochemistry, and in situ hybridization. During this process, tissue embedding and cryopreservation are core elements in ensuring the quality of the sections.

[0003] Currently, researchers typically place embedded mouse brain samples in specialized plastic embedding containers, and then store large quantities of these containers in a low-temperature environment provided by large ultra-low temperature freezers or liquid nitrogen tanks. However, the current storage method has a significant technical drawback: mainstream cryogenic storage equipment (such as cryopreservation racks and cryopreservation boxes) are mostly designed as integral or drawer-type structures. When researchers need to frequently access one or several specific mouse brain samples, they often have to remove the entire storage unit (or even the entire drawer or shelf) from the cryogenic environment and expose it to room temperature for retrieval and storage.

[0004] This operating mode leads to several serious drawbacks: First, it causes a rapid leakage of large amounts of cold air from the storage device and a large influx of hot air from the outside. This not only causes drastic temperature fluctuations and rebounds within the device, posing a serious threat to the preservation quality of all stored samples (especially those not yet retrieved), but repeated temperature shocks also accelerate sample degradation, affecting the accuracy and reproducibility of research data. Second, the compressor or liquid nitrogen consumption system needs to operate under heavy load to quickly restore the set cryogenic environment. This process consumes a huge amount of energy, resulting in unnecessary energy waste and increasing the laboratory's operating costs. Summary of the Invention

[0005] To address the aforementioned technical problems, this invention proposes a mouse brain embedding cassette and its cryogenic storage device for neuroscience research. By storing the embedding cassette separately in a storage drawer, the entire cassette is avoided from being exposed when a single embedding cassette is accessed, effectively reducing cold air leakage. When the storage drawer is pulled out, the cold air is stored in a storage box through a gas storage component. When the storage drawer is retracted, the cold air in the storage box is returned to the storage drawer through the gas storage component, enabling the cold air to be recycled and reused, thus reducing cold air waste.

[0006] The technical solution for achieving the objective of this invention is: a low-temperature storage device for a mouse brain embedding cassette used in neuroscience research, comprising a storage cabinet, the interior of which is provided with a partition component, the partition component comprising: Partition 1 is fixedly installed in the middle of the inner side wall of the storage cabinet. Multiple partitions 2 are fixedly installed at equal intervals on both the left and right sides of partition 1. The side walls of the multiple partitions 2 are fixedly connected to the inner side wall of the storage cabinet. Ventilation holes, there are multiple ventilation holes, and the multiple ventilation holes are respectively opened on the inner side wall of partition two, and the ventilation holes are located inside partition two near the outside. The storage cabinet is equipped with a refrigeration system, which includes: A refrigeration device, which is fixedly installed on the inner bottom wall of the storage cabinet; A vent pipe is provided inside the partition plate 1. The vent pipe is connected to multiple ventilation holes, and the interior of the vent pipe is connected to the output end of the refrigeration device. The storage cabinet has multiple placement components inside, each located on top of a number of partitions. These placement components include: The storage drawer is slidably installed on the top wall of the partition two. A pull ring is fixedly installed on the front wall of the storage drawer. An air inlet is provided on the bottom wall of the storage drawer, and the air inlet is aligned with a nearby air outlet. The storage cabinet is equipped with multiple gas storage components, which are located behind the multiple placement components.

[0007] In some embodiments, the gas storage assembly includes: An air storage box is fixedly installed on the top wall of partition two. Two partitions three are fixedly installed on the side wall of the air storage box near the storage drawer. The upper and lower sides of the two partitions three are fixedly connected to the upper and lower sides of the air storage box. A certain gap is left between the two partitions three and the side wall of the air storage box away from the storage drawer. A piston plate is slidably installed inside the air storage box. The left and right sides of the piston plate are in contact with the side wall of the two partitions three. A pull rod is fixedly installed on the side wall of the piston plate. The end of the pull rod away from the piston plate slides through the side wall of the air storage box and is fixedly connected to the storage drawer. The connecting pipes are multiple in number and are fixedly installed on both sides of the side wall of the air storage box near the storage drawer. The end of the connecting pipe away from the air storage box slides through the side wall of the storage drawer. The multiple connecting pipes are aligned with the cavity formed by the two partitions and the inner side wall of the storage cabinet. The side wall of the connecting pipe is provided with a connecting hole, which communicates with the interior of the storage drawer and the air storage box.

[0008] In some embodiments, the placement component further includes: The track is fixedly installed on the top of the inner side wall of the storage drawer. The inner side wall of the track has a track groove. A folding plate is fixedly connected to the inner side wall of the track groove away from the outside. The left and right ends of the folding plate are slidably installed in corresponding positions inside the track groove. A positioning strip is slidably installed inside the track groove. The positioning strip is fixedly connected to the corresponding side wall of the folding plate. A pull strip is fixedly installed on the top wall of the positioning strip.

[0009] In some embodiments, a sealing strip made of rubber is fixedly installed on the side wall of the piston plate, and a return spring is sleeved on the side wall of the pull rod, with the two ends of the return spring fixedly connected to the corresponding side walls of the piston plate and the gas storage tank, respectively.

[0010] In some embodiments, the interior of the storage drawer is provided with a support assembly, the support assembly including: The system includes multiple connecting plates, each sliding through the side wall of the storage drawer near the air tank. Sliding plates are slidably installed on the outer walls of the connecting plates, and fixedly installed on the inner bottom wall of the storage drawer. Both the connecting plates and the sliding plates are hollow. The connecting plates communicate with the cavities formed by the adjacent side walls of the two partitions. A receiving rod is fixedly installed on the side wall of the sliding plate, and a receiving groove is formed on the top wall of the receiving rod. An extension rod is slidably installed inside the receiving groove. Pressure holes are interconnected on the side walls of the receiving rod and the sliding plate.

[0011] In some embodiments, a placement plate is fixedly installed on the top wall of the extension rod, and multiple ventilation slots are provided on the side wall of the placement plate, with the embedding box placed on top of the placement plate.

[0012] In some embodiments, the support component further includes; A through hole is formed on the inner side wall of the sliding plate, and the through hole is interconnected with multiple pressure holes inside the sliding plate.

[0013] In some embodiments, a limit strip is fixedly installed on the bottom wall of the extension rod, and a vent hole is provided at the bottom end of the side wall of the extension rod, with the top end of the vent hole penetrating through the side wall of the extension rod and communicating with the outside.

[0014] In some embodiments, the storage drawer is internally equipped with a linkage component, which includes: Positioning blocks, there are multiple positioning blocks, and multiple positioning blocks are fixedly installed on the side wall of the connecting pipe; The linkage bar is fixedly installed on the bottom wall of the positioning bar. Magnetic blocks are fixedly installed on both the bottom wall of the linkage bar and the top wall of the positioning block. The magnetic properties of the side walls of the magnetic blocks on the positioning block and the magnetic blocks on the linkage bar that are close to each other are different.

[0015] A mouse brain embedding cassette for neuroscience research, stored using the aforementioned cryogenic storage device, includes: A storage plate, wherein a storage groove is provided on the top wall of the storage plate; A cover plate is placed on top of the storage plate, and an indicator groove is provided on the top wall of the cover plate. The indicator groove is trapezoidal.

[0016] The significant advantages of this invention compared to existing technologies are: Firstly, in this invention, the worker pulls the storage drawer out from the top of partition two. Cold air generated by the refrigeration device is injected into the drawer through the air inlet, venting pipe, and dissipation hole to refrigerate the samples stored inside. When the worker pulls the drawer outwards, the lever pulls the piston plate. Because the sidewall of the piston plate is in contact with the sidewalls of the two partitions three and the inner wall of the storage cabinet, the piston plate can draw the cold air from inside the drawer into the cavity formed between the two partitions three through the connecting hole, storing the cold air inside the drawer. When the worker pushes the drawer into the air storage box, the movement of the piston plate allows the cold air stored between the two partitions three to pass through the partitions. The cold air is fed into the connecting hole through the gap between the inner walls of the storage cabinet and then flows back to the storage drawer, allowing for reuse of the cold air. By storing the embedding cassettes separately in the storage drawer, the entire cassette is avoided from being exposed when a single cassette is accessed, effectively reducing cold air leakage and lowering the heat exchange load. This achieves energy saving and consumption reduction and significantly improves the quality of low-temperature preservation of samples. When the storage drawer is pulled out, the cold air is stored in the storage box through the gas storage component. When the storage drawer is retracted, the cold air in the storage box is sent back to the storage drawer through the gas storage component, enabling the cold air to be recycled and reused, thus reducing cold air waste. This solves the problem of unnecessary energy waste caused by existing refrigeration devices when retrieving embedding cassettes.

[0017] Secondly, this invention, by sliding the folding plate inside the track groove, can seal the top of the storage drawer, creating a sealed environment and preventing cold air from spreading and causing poor refrigeration of the samples inside. Through the cooperation of the positioning strip and the pull strip, the staff can easily push and pull the folding plate to move inside the track groove, and it is also convenient to open or close the top of the storage drawer, thus making it easy for the staff to put the samples into or take them out of the storage drawer.

[0018] Thirdly, this invention utilizes the elasticity of the return spring to support the side wall of the piston plate, allowing the piston plate and the pull rod to jointly pull the storage drawer towards the air storage box. This enables the storage drawer to automatically retract between the two partitions. A sealing strip is fitted onto the side wall of the piston plate, and the friction between the sealing strip and the partition and the side wall of the air storage box effectively reduces the moving speed of the piston plate and suppresses the automatic retraction of the storage drawer.

[0019] Fourthly, in this invention, when the storage drawer moves outward, the position of the connecting pipe remains unchanged because it is fixedly installed on the side wall of the gas storage box. The positioning block and the linkage bar are connected together by the magnetism of the magnetic block, and the position of the positioning bar remains unchanged. As the storage drawer moves, the folding plate is folded. During the process of the storage drawer being pulled outward, the folding plate is opened simultaneously, which further facilitates the staff to take out the embedding box. Since the positioning block and the linkage bar are connected by the magnetism of the magnetic block, the staff can manually close or open the folding plate by pulling the pull bar.

[0020] Fifthly, this invention features a storage slot with a recessed section at the top of the storage plate, facilitating the positioning of samples placed inside and preventing them from shifting around. By placing a cover plate on top of the storage plate, the top is sealed, enhancing sample protection. Utilizing the trapezoidal shape with one end larger than the other, the cover plate makes it easy for staff to distinguish the head and tail of the sample, preventing miscutting due to freezing and potential experimental losses. Attached Figure Description

[0021] The present invention will be further explained below with reference to the accompanying drawings and embodiments: Figure 1 This is a three-dimensional schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram showing the location of the vent holes and vent pipes of the present invention; Figure 3 This is a three-dimensional schematic diagram of the overall structure of the storage drawer of the present invention; Figure 4 This is a three-dimensional schematic diagram of the internal structure of the drawer of this invention; Figure 5 This is a three-dimensional vertical cross-sectional view of the storage drawer and air tank of the present invention; Figure 6 This is a schematic diagram of the internal structure of the cover plate of the present invention; Figure 7 This is a three-dimensional cross-sectional view of the storage drawer and air tank of the present invention; Figure 8 This is a schematic diagram of the internal structure of the sliding plate of the present invention; Figure 9This is a schematic diagram of the internal structure of the receiving rod of the present invention; Figure 10 This is a schematic diagram of the internal structure of the connecting tube of the present invention; Figure 11 This is a schematic diagram of the drawer being pulled out in the present invention.

[0022] Explanation of reference numerals in the attached figures: 1. Storage cabinet; 21. Partition 1; 22. Partition 2; 23. Ventilation vent; 31. Refrigeration unit; 32. Ventilation pipe; 41. Storage drawer; 42. Pull ring; 43. Rail; 44. Rail groove; 45. Folding plate; 46. Positioning strip; 47. Pull bar; 48. Air inlet; 51. Storage plate; 52. Storage slot; 53. Cover plate; 54. Indicator slot; 61. Air tank; 62. Partition 3; 63. Piston plate; 64. Sealing strip; 65. Pull rod; 66. Return spring; 67. Connecting pipe; 68. Connecting hole; 71. Connecting plate; 72. Sliding plate; 73. Receiving rod; 74. Receiving slot; 75. Extension rod; 76. Pressurization hole; 77. Through hole; 78. Limiting strip; 79. Ventilation vent; 8. Placement plate; 91. Positioning block; 92. Linkage strip; 93. Magnetic block. Detailed Implementation

[0023] The present invention will now be described in detail, and the technical solutions in the embodiments of the present invention will be clearly and completely described. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0024] This invention provides, through improvements, a mouse brain embedding cassette and its cryogenic storage device for neuroscience research. The technical solution of this invention is as follows: Example 1 like Figures 4-6 As shown, a mouse brain embedding cassette for neuroscience research includes a storage plate 51 and a cover plate 53. The storage plate 51 is placed inside the storage drawer 41, and a storage groove 52 is provided on the top wall of the storage plate 51. The storage groove 52 makes the top of the storage plate 51 have a recessed part, which facilitates the positioning of the sample placed inside the storage plate 51 and avoids the sample from moving around. The cover plate 53 is placed on top of the storage plate 51. The top wall of the cover plate 53 is provided with an indicator groove 54, which is trapezoidal. By placing the cover plate 53 on top of the storage plate 51, the top of the storage plate 51 is sealed, which improves the protection of the sample. Utilizing the characteristic that one end of the trapezoid is larger and the other end is smaller, when the cover plate 53 is placed on top of the storage plate 51, it is easy for the staff to distinguish the head and tail of the sample, avoiding the situation where the head and tail of the sample are difficult to distinguish due to freezing, which may lead to accidental cutting and experimental loss.

[0025] The specific working method is as follows: the storage slot 52 makes the top of the storage plate 51 have a groove, which facilitates the positioning of the sample placed inside the storage plate 51. By covering the top of the storage plate 51 with the cover plate 53, the top of the storage plate 51 is sealed, which improves the protection of the sample. Utilizing the characteristic that one end of the trapezoid is larger and the other end is smaller, when the cover plate 53 is on the top of the storage plate 51, it is easy for the staff to distinguish the head and tail of the sample, avoiding the situation where the head and tail of the sample are difficult to distinguish due to freezing, which may cause accidental cutting and experimental loss.

[0026] Example 2 like Figures 1-11 As shown, a low-temperature storage device for a mouse brain embedding cassette used in neuroscience research is used to store the mouse brain embedding cassette in Example 1. It includes a storage cabinet 1, and the storage cabinet 1 is provided with a partition component, which includes a partition 21 and a ventilation hole 23. Partition 1 21 is fixedly installed in the middle of the inner side wall of storage cabinet 1. Multiple partitions 22 are fixedly installed at equal intervals on both the left and right sides of partition 1 21. The side walls of the multiple partitions 22 are fixedly connected to the inner side wall of storage cabinet 1. There are multiple air vents 23, which are respectively opened on the inner side wall of the second partition 22. The air vents 23 are located inside the second partition 22 near the outside. The storage cabinet 1 is equipped with a refrigeration system, which includes a refrigeration device 31 and a vent pipe 32. The refrigeration device 31 is fixedly installed on the inner bottom wall of the storage cabinet 1. The refrigeration device 31 is used to generate cold air. The refrigeration device 31 is existing technology and will not be described in detail here. Vent pipe 32 is opened inside partition 21. Vent pipe 32 is connected to multiple air vents 23, and the interior of vent pipe 32 is connected to the output end of refrigeration device 31. The storage cabinet 1 has multiple placement components inside, which are located on top of multiple partitions 22. The placement components include storage drawers 41 and rails 43. The storage drawer 41 is slidably installed on the top wall of the partition 22. The top wall of the storage drawer 41 is open. A pull ring 42 is fixedly installed on the front wall of the storage drawer 41. An air inlet 48 is opened on the bottom wall of the storage drawer 41, and the air inlet 48 is aligned with the adjacent air outlet 23. The pull ring 42 makes it easy for the staff to pull the storage drawer 41 out from the top of the partition 22. The air inlet 48 allows the cold air generated by the refrigeration device 31 to be injected into the interior of the storage drawer 41 through the vent pipe 32 and the air outlet 23 to refrigerate the samples stored inside the storage drawer 41. The track bar 43 is fixedly installed on the top of the inner side wall of the storage drawer 41. The inner side wall of the track bar 43 has a track groove 44. A folding plate 45 is fixedly connected to the inner side wall of the track groove 44 away from the outside. The left and right ends of the folding plate 45 are slidably installed in corresponding positions inside the track groove 44. A positioning strip 46 is slidably installed inside the track groove 44. The positioning strip 46 is fixedly connected to the corresponding side wall of the folding plate 45. A pull strip 47 is fixedly installed on the top wall of the positioning strip 46. By sliding the folding plate 45 inside the track groove 44, the folding plate 45 can seal the top of the storage drawer 41, so that the storage drawer 41 is in a sealed environment, preventing the cold air inside the storage drawer 41 from spreading and causing poor refrigeration effect of the samples inside the storage drawer 41. Through the cooperation of the positioning strip 46 and the pull strip 47, the staff can easily push and pull the folding plate 45 to move inside the track groove 44. It also facilitates the opening or closing of the top of the storage drawer 41, so that the staff can easily put the samples into or take them out of the storage drawer 41. The storage cabinet 1 is equipped with multiple gas storage components, which are located behind the multiple placement components. Each gas storage component includes a gas storage box 61 and a connecting pipe 67. An air storage box 61 is fixedly installed on the top wall of partition 22. Two partitions 3 62 are fixedly installed on the inner wall of the air storage box 61 near the storage drawer 41. The upper and lower sides of the two partitions 3 62 are fixedly connected to the upper and lower sides of the inner wall of the air storage box 61. A certain gap is left between the two partitions 3 62 and the inner wall of the air storage box 61 away from the storage drawer 41. A piston plate 63 is slidably installed inside the air storage box 61. The left and right sides of the piston plate 63 are in contact with the side walls of the two partitions 3 62. A sealing strip 64, made of rubber, is fixedly installed on the side wall of the piston plate 63. A pull rod 65 is fixedly installed on the side wall of the piston plate 63. The pull rod 65 is located away from the piston plate 63. The end of the sliding rod 65 is fixedly connected to the side wall of the air storage box 61 and the storage drawer 41. A return spring 66 is sleeved on the side wall of the pull rod 65. The two ends of the return spring 66 are fixedly connected to the piston plate 63 and the corresponding side wall of the air storage box 61, respectively. By utilizing the elasticity of the return spring 66, the side wall of the piston plate 63 is supported, so that the piston plate 63 and the pull rod 65 pull the storage drawer 41 towards the air storage box 61, thereby enabling the storage drawer 41 to automatically retract between the two partitions 22. A sealing strip 64 is sleeved on the side wall of the piston plate 63. With the help of the friction between the sealing strip 64 and the partition 62 and the side wall of the air storage box 61, the moving speed of the piston plate 63 is effectively reduced, and the automatic retraction amplitude of the storage drawer 41 is suppressed. There are multiple connecting pipes 67, which are fixedly installed on both sides of the side wall of the air storage box 61 near the storage drawer 41. The end of the connecting pipe 67 away from the air storage box 61 slides through the side wall of the storage drawer 41. The multiple connecting pipes 67 are aligned with the cavities formed by the two partitions 62 and the inner side wall of the storage cabinet 1. The side wall of the connecting pipe 67 is provided with a connecting hole 68, which communicates with the interior of the storage drawer 41 and the air storage box 61. When the operator pulls the pull ring 42 to pull the storage drawer 41 outward, the pull rod 65 will pull the piston plate 63 to move together. Because the side wall of the piston plate 63 is aligned with the two partitions 62 and the inner side wall of the storage cabinet 1, the connecting pipe 67 is aligned with the inner side wall of the storage cabinet 41. The side wall of partition 3 62 is attached to the inner side wall of storage cabinet 1. Therefore, piston plate 63 can draw the cold air inside storage drawer 41 into the cavity formed between the two partitions 3 62 through the connecting hole 68 to store the cold air inside storage drawer 41. When the staff pushes storage drawer 41 into air storage box 61, the movement of piston plate 63 sends the cold air stored between the two partitions 3 62 into the interior of connecting hole 68 through the gap between partition 3 62 and the inner side wall of storage cabinet 1. The cold air then flows back into storage drawer 41 through connecting hole 68, reusing the cold air and avoiding waste caused by the cold air spreading everywhere.

[0027] like Figures 4-11As shown, in one embodiment, the storage drawer 41 is provided with a support assembly inside, the support assembly including a connecting plate 71 and a through hole 77; There are multiple connecting plates 71, all of which slide through the side wall of the storage drawer 41 near the air storage box 61. Sliding plates 72 are slidably installed on the outer walls of the multiple connecting plates 71, and are fixedly installed on the inner bottom wall of the storage drawer 41. Both the connecting plates 71 and the sliding plates 72 are hollow, communicating with the cavity formed by the connecting plates 71 and the side walls of the two partitions 62. A receiving rod 73 is fixedly installed on the side wall of the sliding plate 72. A receiving groove 74 is formed on the top wall of the receiving rod 73. An extension rod 75 is slidably installed inside the receiving groove 74. A placement plate 8 is fixedly installed on the top wall of the extension rod 75. Multiple ventilation grooves are formed on the side wall of the placement plate 8. The embedding box is placed on top of the placement plate 8. The receiving rod 73 and the sliding plate... The side wall of 72 is provided with interconnected pressurizing holes 76. The bottom wall of the extension rod 75 is fixedly installed with a limiting strip 78. The bottom end of the side wall of the extension rod 75 is provided with a vent hole 79. The top end of the vent hole 79 passes through the side wall of the extension rod 75 and communicates with the outside. When the storage drawer 41 moves outward, the piston plate 63 shifts to one side of the storage drawer 41, thereby guiding the air on the rear side to the interior of the sliding plate 72 through the connecting plate 71, and entering the interior of the receiving rod 73 through the pressurizing holes 76. As a result, the air pressure inside the sliding plate 72 and the receiving rod 73 increases, causing the extension rod 75 to move upward due to the increase in bottom air pressure. Subsequently, the placement plate 8 and the embedding box on its top also move upward, making it convenient for the staff to take the embedding box out of the storage drawer 41 smoothly. The sliding range of the extension rod 75 is limited by the limiting strip 78 to prevent the extension rod 75 from detaching from the inside of the receiving rod 73. Excess air inside the receiving rod 73 is discharged through the vent hole 79 to prevent the storage drawer 41 from being unable to move outward due to the limited air stored inside the sliding plate 72 and the air behind the piston plate 63 not being able to be discharged. The through hole 77 is opened on the inner side wall of the sliding plate 72, and the through hole 77 is interconnected with multiple pressurization holes 76 inside the sliding plate 72. During the process of the storage drawer 41 moving outward, the connecting plate 71 gradually shifts away from the multiple pressurization holes 76 inside the sliding plate 72, and the multiple pressurization holes 76 are interconnected through the through hole 77, so that the air entering the interior of the sliding plate 72 can enter the interior of multiple receiving rods 73 simultaneously. This can avoid the situation where the pressurization holes 76 near the air storage box 61 cannot rise synchronously due to the lack of air entering because the connecting plate 71 is attached to the inner side wall of the sliding plate 72.

[0028] like Figures 4-11As shown, in one embodiment, the storage drawer 41 is provided with a linkage component inside, which includes a positioning block 91 and a linkage bar 92; There are multiple positioning blocks 91, and multiple positioning blocks 91 are fixedly installed on the side wall of the connecting pipe 67; The linkage bar 92 is fixedly installed on the bottom wall of the positioning bar 46. Magnetic blocks 93 are fixedly installed on the bottom wall of the linkage bar 92 and the top wall of the positioning block 91. The magnetic properties of the adjacent sides of the magnetic blocks 93 on the positioning block 91 and the linkage bar 92 are opposite. When the storage drawer 41 moves outward, the position of the connecting pipe 67 remains unchanged because it is fixedly installed on the side wall of the gas storage box 61. The positioning block 91 and the linkage bar 92 are connected by the magnetism of the magnetic block 93, and the position of the positioning bar 46 remains unchanged. As the storage drawer 41 moves, the folding plate 45 is folded. During the process of pulling the storage drawer 41 outward, the folding plate 45 is opened simultaneously, further facilitating the removal of the embedding box by the staff. Since the positioning block 91 and the linkage bar 92 are connected by the magnetism of the magnetic block 93, the staff can manually close or open the folding plate 45 by pulling the pull bar 47.

[0029] The specific working method is as follows: Cold air generated by the refrigeration unit 31 is injected into the storage drawer 41 through the air inlet 48 via the vent pipe 32 and the ventilation hole 23, keeping the interior of the storage drawer 41 at a low temperature. The pull ring 42 allows the operator to easily pull the storage drawer 41 out from the top of the partition 22. At this time, the pull rod 65 pulls the piston plate 63 to move together. Since the side wall of the piston plate 63 is in contact with the side walls of the two partitions 32 and the inner side wall of the storage cabinet 1, the piston plate 63 can draw the cold air inside the storage drawer 41 into the cavity formed between the two partitions 32 through the connecting hole 68, storing the cold air inside the storage drawer 41. As the storage drawer 41 moves outward, the piston plate 63 shifts to one side of the storage drawer 41, guiding the air behind it through the connecting plate 71 to the interior of the sliding plate 72, and then into the interior of the receiving rod 73 through the pressurization hole 76. This causes the air pressure inside the sliding plate 72 and the receiving rod 73 to increase, resulting in the extension rod 75 being pushed up by the air pressure at the bottom. As the pressure increases, the plate moves upward, and the placement plate 8 and the embedding box on top of it also move upward, making it easier for staff to remove the embedding box from the storage drawer 41. Since the connecting pipe 67 is fixedly installed on the side wall of the gas storage box 61, its position remains unchanged as the storage drawer 41 moves outward. The positioning block 91 and the linkage bar 92 are connected by the magnetism of the magnetic block 93, and the position of the positioning bar 46 remains unchanged. As the storage drawer 41 moves, the folding plate 45 is folded. As the storage drawer 41 is pulled outward, the folding plate 45 is opened simultaneously, further facilitating the removal of the embedding box. When the staff pushes the storage drawer 41 into the gas storage box 61, the movement of the piston plate 63 sends the cold air stored between the two partitions 62 through the gap between the partitions 62 and the inner side wall of the storage cabinet 1 into the connecting hole 68. The cold air then flows back into the storage drawer 41 through the connecting hole 68, reusing the cold air and preventing it from spreading and being wasted.

[0030] The technical means disclosed in this invention are not limited to those described above, but also include technical solutions composed of equivalent substitutions of the above technical features. Matters not covered in this invention are common knowledge to those skilled in the art.

Claims

1. A cryogenic storage device for a mouse brain embedding cassette used in neuroscience research, characterized in that: Includes a storage cabinet (1), the interior of which is provided with partition components, the partition components including: Partition 1 (21) is fixedly installed in the middle of the inner side wall of the storage cabinet (1). Multiple partitions 2 (22) are fixedly installed at equal intervals on both the left and right sides of partition 1 (21). The side walls of multiple partitions 2 (22) are fixedly connected to the inner side wall of the storage cabinet (1). Ventilation holes (23), there are multiple ventilation holes (23), and the multiple ventilation holes (23) are respectively opened on the inner side wall of the partition plate two (22). The ventilation holes (23) are located inside the partition plate two (22) near the outside. The storage cabinet (1) is equipped with a refrigeration system, which includes: A refrigeration device (31) is fixedly installed on the inner bottom wall of the storage cabinet (1); Vent pipe (32), the vent pipe (32) is opened inside the partition (21), the vent pipe (32) is connected to multiple air vents (23), and the interior of the vent pipe (32) is connected to the output end of the refrigeration device (31); The storage cabinet (1) is equipped with multiple placement components inside, which are located on top of multiple partitions (22). The placement components include: A storage drawer (41) is slidably installed on the top wall of the partition (22). A pull ring (42) is fixedly installed on the front wall of the storage drawer (41). An air inlet (48) is opened on the bottom wall of the storage drawer (41). The air inlet (48) is aligned with the nearby air outlet (23). The storage cabinet (1) is equipped with multiple gas storage components, which are located behind the multiple placement components.

2. The cryogenic storage device for a mouse brain embedding cassette used in neuroscience research according to claim 1, characterized in that: The gas storage component includes: An air storage box (61) is fixedly installed on the top wall of the partition two (22). Two partition three (62) are fixedly installed on the side wall of the air storage box (61) near the storage drawer (41). The upper and lower sides of the two partition three (62) are fixedly connected to the upper and lower sides of the air storage box (61). There is a certain gap between the two partition three (62) and the side wall of the air storage box (61) away from the storage drawer (41). A piston plate (63) is slidably installed inside the air storage box (61). The left and right sides of the piston plate (63) are close to the side wall of the two partition three (62). A pull rod (65) is fixedly installed on the side wall of the piston plate (63). The end of the pull rod (65) away from the piston plate (63) slides through the side wall of the air storage box (61) and is fixedly connected to the storage drawer (41). There are multiple connecting pipes (67). The multiple connecting pipes (67) are fixedly installed on both sides of the side wall of the air storage box (61) near the storage drawer (41). The end of the connecting pipe (67) away from the air storage box (61) slides through the side wall of the storage drawer (41). The multiple connecting pipes (67) are respectively aligned with the cavity formed by the two partitions (62) and the inner side wall of the storage cabinet (1). A connecting hole (68) is opened on the side wall of the connecting pipe (67). The connecting hole (68) communicates with the interior of the storage drawer (41) and the air storage box (61).

3. The cryogenic storage device for a mouse brain embedding cassette used in neuroscience research according to claim 1, characterized in that: The placement component also includes: The track bar (43) is fixedly installed on the top of the inner wall of the storage drawer (41). The inner wall of the track bar (43) is provided with a track groove (44). A folding plate (45) is fixedly connected to the inner side wall of the track groove (44) away from the outside. The left and right ends of the folding plate (45) are slidably installed in the corresponding positions inside the track groove (44). A positioning strip (46) is slidably installed inside the track groove (44). The positioning strip (46) is fixedly connected to the corresponding side wall on the folding plate (45). A pull strip (47) is fixedly installed on the top wall of the positioning strip (46).

4. The cryogenic storage device for a mouse brain embedding cassette used in neuroscience research according to claim 2, characterized in that: A sealing strip (64) is fixedly installed on the side wall of the piston plate (63). The sealing strip (64) is made of rubber. A return spring (66) is sleeved on the side wall of the pull rod (65). The two ends of the return spring (66) are fixedly connected to the corresponding side walls of the piston plate (63) and the gas storage box (61), respectively.

5. A cryogenic storage device for a mouse brain embedding cassette used in neuroscience research according to claim 2, characterized in that: The storage drawer (41) is internally provided with a support assembly, which includes: There are multiple connecting plates (71), and multiple connecting plates (71) slide through the side wall of the storage drawer (41) near the air storage box (61). Sliding plates (72) are slidably installed on the outer side wall of the multiple connecting plates (71). The sliding plates (72) are fixedly installed on the inner bottom wall of the storage drawer (41). The interior of the connecting plates (71) and the sliding plates (72) are hollow. The connecting plates (71) are connected to the cavity formed by the side wall of the two partitions (62). A receiving rod (73) is fixedly installed on the side wall of the sliding plate (72). A receiving groove (74) is opened on the top wall of the receiving rod (73). An extension rod (75) is slidably installed inside the receiving groove (74). Pressurization holes (76) are opened on the side walls of the receiving rod (73) and the sliding plate (72).

6. A cryogenic storage device for a mouse brain embedding cassette used in neuroscience research according to claim 5, characterized in that: The top wall of the extension rod (75) is fixedly installed with a placement plate (8), and multiple ventilation grooves are provided on the side wall of the placement plate (8). The embedding box is placed on the top of the placement plate (8).

7. A cryogenic storage device for a mouse brain embedding cassette used in neuroscience research according to claim 5, characterized in that: The support components also include; Through hole (77), the through hole (77) is opened on the inner side wall of the sliding plate (72), and the through hole (77) is connected to multiple pressure holes (76) inside the sliding plate (72).

8. A cryogenic storage device for a mouse brain embedding cassette used in neuroscience research according to claim 5, characterized in that: A limit strip (78) is fixedly installed on the bottom wall of the extension rod (75), and a vent hole (79) is opened at the bottom end of the side wall of the extension rod (75). The top end of the vent hole (79) passes through the side wall of the extension rod (75) and communicates with the outside.

9. A cryogenic storage device for a mouse brain embedding cassette used in neuroscience research according to claim 3, characterized in that: The storage drawer (41) is equipped with a linkage component inside, which includes: Positioning blocks (91), there are multiple positioning blocks (91), and multiple positioning blocks (91) are fixedly installed on the side wall of the connecting pipe (67); Linkage bar (92), the linkage bar (92) is fixedly installed on the bottom wall of the positioning bar (46), and magnetic blocks (93) are fixedly installed on the bottom wall of the linkage bar (92) and the top wall of the positioning block (91). The magnetic sides of the magnetic blocks (93) on the positioning block (91) and the magnetic blocks (93) on the linkage bar (92) are different.

10. A mouse brain embedding cassette for neuroscience research, stored using a cryogenic storage device as described in any one of claims 1-9, characterized in that: include: Storage plate (51), the top wall of which is provided with storage groove (52); A cover plate (53) is placed on top of the storage plate (51). The top wall of the cover plate (53) is provided with an indicator groove (54), which is trapezoidal.

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