A gas distribution structure for a biopharmaceutical fermenter

Abstract

The application discloses a kind of biological medicine fermentation tank gas distribution structures, it is related to biological fermentation tank technical field, including support pedestal, the inside of support pedestal is equipped with fermentation tank body, the front end of support pedestal is equipped with control system, the outside of fermentation tank body is equipped with protective cylinder, the inside of fermentation tank body is provided with three groups of built-in multilayer sensor.The present application, by the synergistic effect of mechanical shearing and flow field guidance, can stabilize the control of bubble diameter, compared with traditional structure gas-liquid contact area is increased, significantly improve the oxygen dissolution rate, at the same time, realize the three-dimensional mixing of fermentation liquid radial and axial, eliminate the "dead volume" in fermentation tank body, make the contact of bacteria and nutrient more sufficient, reduce local metabolic difference, secondly, the setting of sawtooth plate, sawtooth blade forms high-frequency shear force, can efficiently break bubble and not easy to reduce effect due to material adhesion.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological fermentation tank, in particular to a gas distribution structure of biological medicine fermentation tank. BACKGROUND

[0002] In the fermentation production of biological medicine, the growth and metabolism of aerobic microorganisms require a large amount of oxygen dissolved in water or fermentation medium, i.e. dissolved oxygen. However, the solubility of oxygen in water is very low. In order to make the oxygen introduced into the fermentation medium be efficiently utilized by microorganisms, researchers have taken various measures. In the early stage, some people prolonged the residence time of oxygen in the fermentation tank by increasing the height-diameter ratio of the fermentation tank, but this method would cause the need to increase the compressed air pressure, thereby increasing energy consumption and other problems. Later, more people used fluid simulation to select a better dispersing effect of the stirrer to improve the dissolved oxygen, which greatly helps to improve the dissolved oxygen, reduce the stirring power consumption and improve the fermentation production level, and under the condition of ensuring the production quality of the product, it has become a commonly used way. In this process, the gas distributor plays a crucial role, which directly affects the air distribution and dispersion effect in the tank.

[0003] In the prior art, the gas distributors used in the fermentation tank mainly have the following types: single pipe type gas distributor, ring type multi-hole pipe gas distributor and Venturi jet pipe gas distributor. However, these traditional gas distributors have many shortcomings, such as the single pipe air distributor has poor air dispersion effect and is not energy-saving; the ring pipe multi-hole distributor and the filter core type microbubble distributor make the air discharge from the small holes or micro-holes, but the air quickly gathers again, which easily causes the small hole to be blocked, affects the distribution effect, and even causes incomplete sterilization and fermentation production pollution. At the same time, with the development of biochemical technology and the increasing demand for biochemical products, the fermentation tank gradually develops towards large and medium-sized, which puts forward higher requirements for the gas-liquid dispersion of the gas distributor, and it needs to have better dispersion effect, anti-blocking ability and energy-saving performance. SUMMARY

[0004] The purpose of the present application is to provide a gas distribution structure of biological medicine fermentation tank to solve the problems in the background art.

[0005] To achieve the above purpose, the present application provides the following technical scheme: a gas distribution structure of biological medicine fermentation tank, comprising a support seat, a fermentation tank body is installed in the inside of the support seat, a control system is installed at the front end of the support seat, a protective cylinder is sleeved on the outside of the fermentation tank body, three groups of built-in multi-layer sensors are arranged in the inside of the fermentation tank body, a bubble breaking mechanism is arranged in the inside of the fermentation tank body, and a layered gas distribution mechanism is arranged on one side of the support seat.

[0006] The bubble breaking mechanism comprises a variable speed motor, a stirring shaft rod is rotatably installed in the interior of the fermentation tank body, three groups of flow guide ring plates are installed on the inner wall of the fermentation tank body, a plurality of groups of discs are installed on the outer wall of the stirring shaft rod, a plurality of groups of arc plates are installed on the outer wall of the plurality of groups of discs, three groups of disturbance plates are installed on the outer wall of three groups of discs, two groups of sawtooth plates are installed on both ends of each group of disturbance plates, and concave flow guide grooves are installed on the inner wall of three groups of flow guide ring plates.

[0007] Preferably, the lower end of the variable speed motor is fixed with the upper end of the fermentation tank body, the upper end of the stirring shaft rod is fixed with the output end of the variable speed motor, each three groups of disturbance plates are arranged in the middle of a group of flow guide ring plates, and the variable speed motor is signal connected with the control system.

[0008] Preferably, the built-in multi-layer sensor comprises a dissolved oxygen sensor, a pH sensor, a temperature sensor and a turbidity sensor, and the built-in multi-layer sensor is signal connected with the control system.

[0009] Preferably, the layered gas distribution mechanism comprises a base, three groups of circular ring pipes and an exhaust pipe, a gas source assembly is installed on the upper end of the base, a vertical pipe is installed on the upper end opening of the gas source assembly, a sterilization filter is installed on the upper end of the gas source assembly, three groups of long pipes are installed through the outer wall of the protective cylinder, an electromagnetic valve is installed on one end of each of the three groups of long pipes, a plurality of groups of air jet heads are installed on the inner wall of three groups of circular ring pipes, and a concave pipe is installed on the upper end of the sterilization filter.

[0010] Preferably, the base is located on one side of the support frame base, the three groups of circular ring pipes are equidistantly distributed in the interior of the fermentation tank body and are respectively located above the three groups of flow guide ring plates, the other ends of the three groups of long pipes are fixedly communicated with the outer wall of the fermentation tank body, the protruding parts of the three groups of circular ring pipes are fixedly communicated with the inner wall of the fermentation tank body, and the three groups of circular ring pipes are communicated with the corresponding long pipes.

[0011] Preferably, the exhaust pipe is located on one side of the protective cylinder, the other end of the concave pipe is fixed with the upper end of the exhaust pipe, the gas source assembly and the sterilization filter are signal connected with the control system, and the three groups of electromagnetic valves are signal connected with the control system.

[0012] Preferably, two groups of circular ring seats are installed on the outer wall of the exhaust pipe, and a connecting rod is installed on the outer wall of each group of circular ring pipes.

[0013] Preferably, the protruding parts of the two groups of circular ring seats are fixed with the outer wall of the protective cylinder, and the other ends of each group of connecting rods are fixed with the inner wall of the fermentation tank body.

[0014] Preferably, the other side of the support seat is provided with a heat exchange system, the outer wall of the protective cylinder is provided with two groups of connecting pipes, and the outer wall of the fermentation tank body is provided with a spiral heating pipe.

[0015] Preferably, one end of each of the two groups of connecting pipes is fixed with the two end openings of the spiral heating pipe, and the other end of each of the two groups of connecting pipes is fixed with the liquid outlet and the liquid inlet of the heat exchange system, and the heat exchange system is signal-connected with the control system.

[0016] Compared with the prior art, the present application has the following beneficial effects:

[0017] 1、In the present application, by setting the bubble breaking mechanism, through the synergistic effect of mechanical shearing and flow field guidance, the diameter of the bubble can be stably controlled, the gas-liquid contact area is increased compared with the traditional structure, the oxygen dissolution rate is significantly improved, at the same time, the radial and axial three-dimensional mixing of the fermentation liquid is realized, the "dead volume" in the fermentation tank body is eliminated, the contact between the bacteria and the nutrients is more sufficient, the local metabolic difference is reduced, secondly, the setting of the sawtooth plate, the sawtooth-shaped edge forms high-frequency shearing force, which can efficiently break the bubbles and is not easy to reduce the effect due to material adhesion.

[0018] 2、In the present application, the setting of the disturbance plate forms local turbulence when rotating, avoiding bubble aggregation and fusion; the setting of the flow guide ring plate divides the tank into multiple independent flow fields, forcing the bubbles to move along the preset path, prolonging the residence time, and the setting of the concave flow guide groove guides the bubbles to spiral upward, increasing the gas-liquid contact time and avoiding the bubbles from escaping along the tank wall; the radial thrust generated by the arc-shaped plate when rotating with the shaft drives the fermentation liquid to flow towards the tank wall, forming a convection cycle with the flow guide ring plate, and multiple discs are distributed along the shaft to realize layered stirring at different heights, adapting to the three-dimensional mixing needs of large-scale fermentation tanks.

[0019] 3、In the present application, by setting the layered gas distribution mechanism, "on-demand distribution" is realized in three independent gas distribution circuits, the gas inlet amount can be dynamically adjusted according to the oxygen dissolution needs at different heights, the setting of the sterilization filter integrates sterilization filtration and sterile exhaust system to ensure the sterility of the incoming gas and reduce the risk of fermentation pollution, which can avoid the "excessive delivery" problem of traditional single-path gas supply, improve the gas utilization rate and reduce the energy consumption of compressed air.

[0020] 4、In the present application, the bubble breaking mechanism and the layered gas distribution mechanism are connected through the process of "gas supply-breaking-flow guiding", forming a functional closed loop: the layered gas distribution provides uniform initial gas source, the bubble breaking mechanism refines it into micro-bubbles, and the flow guiding structure prolongs the mass transfer time, the three work together to improve the oxygen dissolution efficiency compared with the traditional system, while reducing energy consumption, especially suitable for large-scale fermentation production of high-oxygen-demanding biological drugs. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 It is a perspective view of a gas distribution structure of a biological medicine fermentation tank according to the present application;

[0022] Figure 2 It is a partial semi-section perspective view of a gas distribution structure of a biological medicine fermentation tank according to the present application;

[0023] Figure 3 It is a front structure schematic view of a gas distribution structure of a biological medicine fermentation tank according to the present application;

[0024] Figure 4 It is a semi-section perspective view of a fermentation tank and a bubble breaking mechanism of a gas distribution structure of a biological medicine fermentation tank according to the present application;

[0025] Figure 5 It is a partial bubble breaking mechanism perspective view of a gas distribution structure of a biological medicine fermentation tank according to the present application;

[0026] Figure 6 It is an up view of a flow guide ring plate of a gas distribution structure of a biological medicine fermentation tank according to the present application;

[0027] Figure 7 It is a disturbance plate structure schematic view of a gas distribution structure of a biological medicine fermentation tank according to the present application;

[0028] Figure 8 It is a layered gas distribution mechanism perspective view of a gas distribution structure of a biological medicine fermentation tank according to the present application;

[0029] Figure 9 It is a system view of a gas distribution structure of a biological medicine fermentation tank according to the present application.

[0030] In the figure:

[0031] 1, support frame; 11, fermentation tank body; 12, control system; 13, protection cylinder; 14, cold and heat exchange system; 15, connecting pipe; 16, spiral heating pipe; 17, built-in multi-layer sensor; 2, bubble breaking mechanism; 21, variable speed motor; 22, stirring shaft; 23, flow guide ring plate; 24, disc; 25, arc plate; 26, disturbance plate; 27, sawtooth plate; 28, concave flow guide groove; 3, layered gas distribution mechanism; 31, base; 32, gas source assembly; 33, circular ring pipe; 331, connecting rod; 34, circular ring base; 35, vertical pipe; 36, exhaust pipe; 37, sterilization filter; 38, long pipe; 39, electromagnetic valve; 310, air jet head; 311, concave pipe. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present application.

[0033] Embodiment one: refer to Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7 , Figure 8 , and Figure 9 As shown in the figure: a kind of biological medicine fermentation tank gas distribution structure, including support seat 1, fermentation tank body 11 is installed in the inside of support seat 1, control system 12 is installed at the front end of support seat 1, protective cylinder 13 is sleeved on the outside of fermentation tank body 11, three groups of built-in multi-layer sensors 17 are arranged in the inside of fermentation tank body 11, bubble breaking mechanism 2 is arranged in the inside of fermentation tank body 11, and layered gas distribution mechanism 3 is arranged on one side of support seat 1;

[0034] Bubble breaking mechanism 2 includes variable speed motor 21, stirring shaft 22 is rotatably installed in the inside of fermentation tank body 11, three groups of flow guide ring plates 23 are installed on the inner wall of fermentation tank body 11, a plurality of discs 24 are installed on the outer wall of stirring shaft 22, a plurality of arc plates 25 are installed on the outer wall of a plurality of discs 24, three groups of disturbance plates 26 are installed on the outer wall of three groups of discs 24, two groups of sawtooth plates 27 are installed at the two ends of each group of disturbance plates 26, concave flow guide grooves 28 are installed on the inner wall of three groups of flow guide ring plates 23, the lower end of variable speed motor 21 is fixed to the upper end of fermentation tank body 11, and the upper end of stirring shaft 22 is fixed to the output end of variable speed motor 21. Three groups of disturbance plates 26 are arranged in the middle of one group of flow guide ring plates 23.

[0035] Variable speed motor 21 is signal connected with control system 12, built-in multi-layer sensors 17 include dissolved oxygen DO sensor, pH sensor, temperature sensor and turbidity sensor, and built-in multi-layer sensors 17 are signal connected with control system 12.

[0036] In the embodiment, support seat 1 is used as the bearing basis of the whole system, fermentation tank body 11 is fixedly installed in the inside of support seat 1, control system 12 (core control hub) is integrated at the front end, layered gas distribution mechanism 3 is arranged on one side, cold and heat exchange system 14 is arranged on the other side, modular layout is formed, installation and maintenance are facilitated, fermentation tank body 11 is used as fermentation core container, protective cylinder 13 (having heat preservation and safety protection functions) is sleeved on the outside of fermentation tank body 11.

[0037] Bubble breaking mechanism 2 (stirring shaft 22, guide ring plate 23, etc.), responsible for bubble refinement and flow field optimization;

[0038] Variable speed motor 21 (installed at the top of the fermentation tank) provides power for stirring shaft 22, and the speed is adjusted by control system 12 (to adapt to different fermentation stages); the outer wall of stirring shaft 22 is fixed with multiple groups of discs 24, and the edge of disc 24 is provided with arc plate 25 (which pushes the fermentation liquid to flow radially when the shaft rotates, enhancing the mixing effect);

[0039] Three groups of disturbance plates 26 are arranged on the outer wall of each of the three groups of discs 24, and sawtooth plates 27 (made of food-grade stainless steel with an edge angle of 30°) are arranged at both ends of the plate body. When rotating, the bubbles are broken to a diameter of 100-300 μm through shearing action; disturbance plate 26 is accurately located in the middle of the three groups of guide ring plates 23, forming a synergistic structure of “rotary breaking + fixed guide flow”;

[0040] The inner wall of guide ring plate 23 (fixed to the inner wall of the tank and parallel to the horizontal plane) is processed with concave guide groove 28 (uniformly distributed along the circumference), which guides the broken bubbles to spiral upward along the groove body, prolonging the gas-liquid contact time;

[0041] The three groups of guide ring plates 23 are distributed at equal intervals, dividing the fermentation tank body 11 into three independent flow field areas to avoid uneven distribution caused by vertical movement of bubbles;

[0042] The built-in multi-layer sensor 17 (distributed in three groups along the height direction) includes dissolved oxygen DO sensor, pH sensor, temperature sensor and turbidity sensor, which can collect fermentation parameters in each layer in real time;

[0043] Dissolved oxygen (DO) sensor: DO probes are installed at different heights to monitor the dissolved oxygen level in each layer in real time (such as the bottom where the dissolved oxygen is higher due to aeration, and the top where the dissolved oxygen may be lower due to consumption by bacteria), reflecting the layered differences in gas-liquid mass transfer and bacterial activity;

[0044] pH sensor: multiple pH probes can record the pH changes in each layer in real time, and timely detect local metabolic abnormalities (such as pH surge caused by bacterial death at the bottom);

[0045] Temperature sensor: large-scale fermentation tanks may have temperature stratification, and multiple temperature probes can monitor the uniformity of the heating / cooling system;

[0046] Turbidity sensor: real-time monitoring of bacterial concentration in each layer through light scattering principle, indirectly reflecting the growth state;

[0047] The precise stratified gas supply reduces invalid gas consumption, and the low speed mode of the variable speed motor 21 (the speed can be reduced by 30% in the non-logarithmic period) reduces the overall energy consumption by 25%. It is suitable for 500-5000L fermentation tanks of different scales, and the parameters can be flexibly adjusted according to the type of microorganisms (bacteria, fungi, yeast, etc.), and it has strong versatility.

[0048] The crushing effect of the sawtooth plate 27 and the disturbance plate 26 increases the gas-liquid contact area, and the spiral flow field design of the concave flow guide groove 28 improves the oxygen dissolution rate compared with the traditional structure.

[0049] The stratified gas distribution and the partition control of the flow guide ring plate 23 control the oxygen dissolution deviation of each layer in the fermentation tank body 11, and avoid metabolic abnormalities caused by local anoxia.

[0050] Real-time monitoring and dynamic adjustment of multiple sensors ensure the stability of pH, temperature and other parameters, and are suitable for the fermentation needs of high sensitivity biological drugs (such as vaccines and recombinant proteins).

[0051] Modular design allows the bubble breaking mechanism and stratified gas distribution assembly to be separately disassembled and cleaned, meeting the GMP cleaning requirements.

[0052] The control system 12 integrates all operating parameters, supports remote monitoring and data tracing, and reduces the cost of manual intervention.

[0053] Example two: as Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 8 and Figure 9As shown, the layered air distribution mechanism 3 includes a base 31, three sets of circular ring pipes 33 and an exhaust pipe 36. The upper end of the base 31 is provided with an air source assembly 32. The upper end of the air source assembly 32 is provided with a vertical pipe 35. The upper end of the air source assembly 32 is provided with a sterilization filter 37. Three sets of long pipes 38 are penetratingly installed on the outer wall of the protective cylinder 13. One end of each of the three sets of long pipes 38 is provided with an electromagnetic valve 39. The inner wall of each of the three sets of circular ring pipes 33 is provided with a plurality of air injection heads 310. The upper end of the sterilization filter 37 is provided with a concave pipe 311. The base 31 is located on one side of the support frame base 1. The three sets of circular ring pipes 33 are equidistantly distributed inside the fermentation tank body 11 and are respectively located above the three sets of flow guide ring plates 23. The other end of each of the three sets of long pipes 38 is fixedly communicated with the outer wall of the fermentation tank body 11. The protruding portions of the three sets of circular ring pipes 33 are fixedly communicated with the inner wall of the fermentation tank body 11. The three sets of circular ring pipes 33 are communicated with the corresponding long pipes 38. The exhaust pipe 36 is located on one side of the protective cylinder 13. The other end of the concave pipe 311 is fixed with the upper end of the exhaust pipe 36. The air source assembly 32 and the sterilization filter 37 are signal connected with the control system 12. The three sets of electromagnetic valves 39 are signal connected with the control system 12. The outer wall of the exhaust pipe 36 is provided with two sets of circular ring seats 34. The outer wall of each of the two sets of circular ring pipes 33 is provided with a connecting rod 331. The protruding portions of the two sets of circular ring seats 34 are fixed with the outer wall of the protective cylinder 13. The other end of each of the connecting rods 331 is fixed with the inner wall of the fermentation tank body 11.

[0054] In this embodiment, the air source assembly 32 (including an air compressor and a flow controller) on the base 31 provides sterile gas. The sterilization filter 37 (adopting a 0.22 μm filter core) removes microorganisms to ensure the cleanliness of the incoming air.

[0055] The concave pipe 311 connects the sterilization filter 37 and the exhaust pipe 36 to realize the sterile discharge of excess gas. The circular ring seat 34 fixes the position of the exhaust pipe 36 to ensure the stability of the exhaust.

[0056] The three sets of circular ring pipes 33 (equidistantly distributed in the tank and respectively located above the flow guide ring plates 23) are fixed to the inner wall of the tank through the connecting rods 331. The inner wall is uniformly provided with 20 air injection heads 310 to avoid liquid backflow.

[0057] The three sets of long pipes 38 penetrate the tank wall and the protective cylinder 13. One end is connected with the circular ring pipe 33 and the other end is provided with an electromagnetic valve 39 (independently controlled by the control system 12 to control the air intake of each layer). The dynamic adjustment of "more supply at the bottom and less supply at the top" is realized (for example, the air intake at the bottom is 50% higher than that at the top during the logarithmic phase of fermentation).

[0058] The sterilization filter 37 and the sterile exhaust design reduce the risk of contamination, so that the contamination rate of miscellaneous bacteria can be controlled.

[0059] Example Three: As Figure 1 , Figure 2 ,Figure 3 and Figure 9 As shown in the figure, the other side of the support seat 1 is provided with a cold-heat exchange system 14, the outer wall of the protective cylinder 13 is provided with two groups of connecting pipes 15, the outer wall of the fermentation tank body 11 is provided with a spiral heating pipe 16, one end of the two groups of connecting pipes 15 is fixed with the two end openings of the spiral heating pipe 16 respectively, the other end of the two groups of connecting pipes 15 is fixed with the liquid outlet and the liquid inlet of the cold-heat exchange system 14 respectively, and the cold-heat exchange system 14 is signal connected with the control system 12.

[0060] In the embodiment, the cold-heat exchange system 14: forms a closed loop circuit with the spiral heating pipe 16 on the outer wall of the fermentation tank through the two groups of connecting pipes 15, and heat conducting oil or constant temperature water is introduced; in cooperation with the built-in temperature sensor, the fermentation temperature is accurately controlled (such as 37℃±0.5℃), and the inactivation of microorganisms due to temperature fluctuation is avoided;

[0061] The built-in multi-layer sensor 17 collects the dissolved oxygen, pH, temperature and turbidity data of each layer in real time and transmits them to the control system 12; the control system 12 analyzes the data through an algorithm, automatically adjusts the rotating speed of the variable speed motor 21, the opening degree of the electromagnetic valve 39 and the power of the cold-heat exchange system 14, and forms a closed loop control of “monitoring-analysis-execution”.

[0062] The working principle of the present application is as follows: first, before fermentation, the control system 12 receives preset parameters (such as target temperature 37℃, dissolved oxygen threshold 30%-50%, pH range 6.5-7.2) and completes self-checking of the equipment:

[0063] The cold-heat exchange system 14 is started, heat conducting medium is introduced into the spiral heating pipe 16 through the connecting pipe 15, and the fermentation tank body 11 is preheated to the set temperature;

[0064] The gas source assembly 32 performs airtightness test, and the sterilization filter 37 starts the self-cleaning program to ensure that the gas passage is sterile;

[0065] The built-in multi-layer sensor 17 is calibrated to ensure the detection accuracy of parameters such as dissolved oxygen, pH and temperature;

[0066] Then, the compressed air generated by the gas source assembly 32 (air compressor) first enters the sterilization filter 37, and the 0.22μm filter element removes microorganisms, dust and other impurities, and the purified sterile gas is divided into two paths:

[0067] The main stream gas enters the three groups of long pipes 38 through the vertical pipe 35;

[0068] The excess gas is introduced into the exhaust pipe 36 through the concave pipe 311, and the exhaust pipe 36 fixed by the circular ring seat 34 is stably discharged to avoid excessive pressure in the tank;

[0069] Layered precise air supply:

[0070] The control system 12 adjusts the opening degree of the three groups of electromagnetic valves 39 according to the fermentation stage (such as the initial stage, the logarithmic phase, and the stationary phase) instructions:

[0071] In the initial fermentation stage (bacterial adaptation stage), the bottom circular ring pipe 33 accounts for 40% of the gas inlet amount, the middle layer accounts for 30%, and the upper layer accounts for 30%, to avoid gas waste;

[0072] In the logarithmic phase (high oxygen demand stage), the bottom gas inlet amount is increased to 60%, the middle layer is 25%, and the upper layer is 15%, and the gas is sprayed into the fermentation liquid through the air jet head 310 (inclined 45° downward), and the gas impacts the liquid surface to form initial bubbles;

[0073] The circular ring pipe 33 is fixed to the inner wall of the tank through the connecting rod 331 to ensure the stability of the air jet position and form a space cooperation with the flow guide ring plate 23;

[0074] Primary crushing and disturbance:

[0075] The variable speed motor 21 drives the stirring shaft rod 22 to rotate (speed 100-300 rpm), which drives the disc 24 and the disturbance plate 26 on the outer wall to rotate:

[0076] The sawtooth plate 27 (30° blade) at both ends of the disturbance plate 26 cuts the bubbles at high speed, crushing the initial bubbles (diameter 1-3 mm) to 500-800 μm;

[0077] The arc-shaped plate 25 rotates with the disc 24, pushing the fermentation liquid to form a radial ring flow, so that the bubbles are preliminarily diffused;

[0078] Secondary refinement and flow guide:

[0079] The crushed bubbles rise to the area of the flow guide ring plate 23, and the concave flow guide groove 28 (U-shaped cross section) guides the bubbles to rise along a spiral path:

[0080] The flow guide ring plate 23 divides the tank into three independent flow fields to avoid vertical bubble movement;

[0081] The spiral rising path prolongs the bubble residence time by more than 30%, fully contacts with the fermentation liquid, further refines the bubbles to 100-300 μm, and increases the gas-liquid contact area by more than 5 times;

[0082] Finally, full-tank mixing enhancement:

[0083] The multiple discs 24 and the flow guide ring plates 23 are alternately distributed to form a cycle of "stirring-flow guiding-stirring again":

[0084] The lower bubbles are broken by the disturbance plate 26 and guided to the middle layer by the lower flow guide ring plate 23;

[0085] The middle layer bubbles are broken again under secondary stirring and enter the upper layer through the middle layer flow guide ring plate 23, and finally realize uniform distribution of bubbles in the whole tank;

[0086] Real-time data acquisition:

[0087] Three groups of built-in multi-layer sensors 17 (located in upper, middle, and lower layers) collect data every second:

[0088] Dissolved oxygen sensors monitor oxygen concentration in each layer to determine if there is local hypoxia;

[0089] pH sensors feedback metabolic acid-base changes (such as pH drop caused by bacterial acid production);

[0090] Temperature sensors track changes in fermentation broth heat (microbial metabolic heat production or environmental heat dissipation);

[0091] Intelligent feedback regulation:

[0092] The control system 12 compares real-time data with preset thresholds and automatically executes adjustment instructions:

[0093] If the bottom layer dissolved oxygen is less than 30%, increase the bottom layer electromagnetic valve 39 opening degree, and increase the variable speed motor 21 speed (increase the crushing efficiency);

[0094] If the middle layer pH is less than 6.5, link the feed system (not shown) to add alkali, and reduce the corresponding area gas intake (reduce CO2 dissolution);

[0095] Temperature deviation from 37℃±0.5℃: instruct the cold and heat exchange system 14 to adjust the heat transfer medium flow, balance the temperature through the spiral heating pipe 16;

[0096] The cold and heat exchange system 14 forms a closed loop with the two groups of connecting pipes 15 and the spiral heating pipe 16:

[0097] When the sensor detects that the temperature is too high (such as > 38℃): low-temperature heat transfer oil is introduced, and heat is absorbed through the spiral pipe;

[0098] Temperature is too low (such as < 36℃): switch to high-temperature medium to release heat to maintain constant temperature;

[0099] The protective cylinder 13 reduces heat exchange between the tank wall and the outside world, and assists in maintaining temperature stability.

[0100] Although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments, or make equivalent replacements for part of the technical features, and any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A gas distribution structure for biopharmaceutical fermentors comprising a support pedestal (1) characterized in that: The inside of the support frame seat (1) is provided with a fermentation tank body (11), the front end of the support frame seat (1) is provided with a control system (12), the outer side of the fermentation tank body (11) is provided with a protective cylinder (13), the inside of the fermentation tank body (11) is provided with three groups of built-in multi-layer sensors (17), the inside of the fermentation tank body (11) is provided with a bubble breaking mechanism (2), and one side of the support frame seat (1) is provided with a layered gas distribution mechanism (3). The bubble breaking mechanism (2) comprises a variable speed motor (21), the inside of the fermentation tank body (11) is rotatably provided with a stirring shaft rod (22), the inner wall of the fermentation tank body (11) is provided with three groups of flow guide ring plates (23), the outer wall of the stirring shaft rod (22) is provided with a plurality of disc plates (24), the outer wall of the plurality of disc plates (24) is provided with a plurality of arc plates (25), the outer wall of three disc plates (24) is provided with three groups of disturbance plates (26), and the two ends of each group of disturbance plates (26) are provided with two groups of sawtooth plates (27). The inner wall of the three groups of flow guide ring plates (23) is provided with a concave flow guide groove (28). The layered gas distribution mechanism (3) comprises a base (31), three groups of circular ring pipes (33) and an exhaust pipe (36), the upper end of the base (31) is provided with a gas source assembly (32), the upper end of the gas source assembly (32) is provided with a vertical pipe (35), the upper end of the gas source assembly (32) is provided with a sterilization filter (37), the outer wall of the protective cylinder (13) is provided with three groups of long pipes (38), one end of each of the three groups of long pipes (38) is provided with an electromagnetic valve (39), the inner wall of each of the three groups of circular ring pipes (33) is provided with a plurality of jet heads (310), and the upper end of the sterilization filter (37) is provided with a concave pipe (311).

2. The gas distribution structure for a biopharmaceutical fermenter of claim 1, wherein: The lower end of the variable speed motor (21) is fixed to the upper end of the fermentation tank body (11), the upper end of the stirring shaft rod (22) is fixed to the output end of the variable speed motor (21), every three groups of disturbance plates (26) are arranged in the middle of one group of flow guide ring plates (23), and the variable speed motor (21) is signal connected with the control system (12).

3. The gas distribution structure for a biopharmaceutical fermenter of claim 1, wherein: The built-in multi-layer sensor (17) comprises a dissolved oxygen (DO) sensor, a pH sensor, a temperature sensor and a turbidity sensor, and is signal connected with the control system (12).

4. The gas distribution structure for a biopharmaceutical fermenter of claim 1, wherein: The base (31) is located on one side of the support frame seat (1), three groups of circular ring pipes (33) are equidistantly distributed in the inside of the fermentation tank body (11) and are located above three groups of flow guide ring plates (23) respectively, the other end of each of the three groups of long pipes (38) is fixedly communicated with the outer wall of the fermentation tank body (11), the protruding portions of the three groups of circular ring pipes (33) are fixedly communicated with the inner wall of the fermentation tank body (11), and the three groups of circular ring pipes (33) are communicated with the corresponding long pipes (38).

5. The gas distribution structure for a biopharmaceutical fermenter of claim 4, wherein: The exhaust pipe (36) is located on one side of the protective cylinder (13), the other end of the concave pipe (311) is fixed with the upper end of the exhaust pipe (36), the air source assembly (32) and the sterilization filter (37) are signal connected with the control system (12), and the three groups of electromagnetic valves (39) are signal connected with the control system (12).

6. The gas distribution structure for a biopharmaceutical fermenter of claim 5, wherein: The outer wall of the exhaust pipe (36) is provided with two groups of circular ring seats (34), and the outer wall of each group of circular ring pipes (33) is provided with a connecting rod (331).

7. The gas distribution structure for a biopharmaceutical fermenter of claim 6, wherein: The protruding parts of the two groups of circular ring seats (34) are fixed with the outer wall of the protective cylinder (13), and the other end of each group of connecting rods (331) is fixed with the inner wall of the fermentation tank body (11).

8. The gas distribution structure for a biopharmaceutical fermenter of claim 1, wherein: The other side of the support frame seat (1) is provided with a cold and heat exchange system (14), the outer wall of the protective cylinder (13) is provided with two groups of connecting pipes (15), and the outer wall of the fermentation tank body (11) is provided with a spiral heating pipe (16).

9. The gas distribution structure for a biopharmaceutical fermenter of claim 8, wherein: One end of each of the two groups of connecting pipes (15) is fixed with the two end openings of the spiral heating pipe (16), the other end of each of the two groups of connecting pipes (15) is fixed with the liquid outlet and the liquid inlet of the cold and heat exchange system (14), and the cold and heat exchange system (14) is signal connected with the control system (12).

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