Sweat biomarker monitoring multi-mode sensor preparation method and multi-mode sensor

Abstract

The invention discloses a sweat biomarker monitoring multi-mode sensor preparation method and a multi-mode sensor. The preparation method comprises the following steps: preparing a first packaging layer; preparing a lactic acid detection piece; preparing a glucose detection piece; preparing a sweat amount measuring assembly; preparing a second packaging layer, taking a PDMS curing agent and a PDMS basic component solution, stirring, pouring into a mold, and putting into a drying oven for curing; respectively guiding the lactic acid detection piece and the glucose detection piece into the glucose content test bin and the lactic acid content test bin; placing the sweating amount measuring assembly between the first packaging layer and the second packaging layer, and packaging the second packaging layer part and the first packaging layer part into a whole by using a packaging agent; bonding a double-sided adhesive tape with a release layer to the outer surface of the second packaging layer; the second packaging layer and the double-sided adhesive tape are provided with sweat leading-in holes, and the contact liquid guide line extends out of the outer end face of the double-sided adhesive tape through the sweat leading-in holes.

Description

Technical Field

[0001] This application relates to the field of medical and health testing device technology, specifically to the preparation method of a multi-mode sensor for monitoring sweat biomarkers and the multi-mode sensor itself. Background Technology

[0002] Sweat contains abundant biomarkers, such as metabolic products (glucose, lactate, etc.). The levels of these biomarkers indicate the risk of developing underlying diseases. Glucose in sweat can reflect the body's glucose levels. Therefore, sweat is the most promising non-invasive alternative to blood samples for assessing blood glucose levels. Measuring glucose levels in sweat facilitates adaptive adjustments that are beneficial for managing diabetes.

[0003] Sweat lactate is involved in multiple metabolic pathways in the body, providing valuable insights into energy metabolism and making it a promising candidate for assessing physical performance in sports, military, and healthcare applications. Its production increases with exercise intensity and is directly associated with acidosis, dehydration, and electrolyte imbalances. Therefore, real-time monitoring of the concentration of target biomarkers in sweat facilitates preliminary, non-invasive assessment of metabolic and physiological states, enabling personalized healthcare interventions and early detection of health problems.

[0004] When the rate of sweat loss is unclear, analyzing sweat glucose and lactate may be significantly biased. At the same time, monitoring sweat volume is also crucial for improving athletic performance, ensuring physical health, and helping with disease management.

[0005] Therefore, providing such multi-parameter, multi-mode sensor devices and manufacturing processes, which can meet the requirements of non-invasive evaluation and have high reliability and sensitivity, is highly desirable. Summary of the Invention

[0006] This invention addresses the above-mentioned problems by proposing a method for preparing a multi-mode sensor for monitoring sweat biomarkers, aiming to solve the technical issues in the background art.

[0007] To achieve the above objectives, the present invention provides a method for preparing a multi-mode sensor for monitoring sweat biomarkers, the method comprising the following steps: To prepare the first encapsulation layer, polydimethylsiloxane curing agent and PDMS basic component solution are mixed in a certain proportion, stirred, and then placed in a mold. The mold is placed in an oven for curing, and the first encapsulation layer is formed after curing. The first encapsulation layer is provided with a glucose content testing chamber and a lactic acid content testing chamber. To prepare a lactic acid detection element, Fe3O4 / DEX / PDA@Au (Raman reporters)@Au magnetic gold particles were freeze-dried to obtain the corresponding powdered first magnetic gold particle product. To prepare a glucose detection device, Fe3O4 / DEX / PDA@Au(Raman reporters)@Au-MPBA magnetic gold particles were freeze-dried to obtain a corresponding powdered second magnetic gold particle product. A sweat volume measurement component is prepared by processing a sweat detection solution onto a solvent carrier, arranging multiple processed solvent carriers in a predetermined array, and connecting liquid guide lines to connect multiple solvent carriers in the array in series. One or more solvent carriers have contact liquid guide lines extending outward from their centers. To prepare the second encapsulation layer, take PDMS curing agent and PDMS basic component solution, stir them and pour them into a mold, put them in an oven for curing, and the second encapsulation layer is formed after curing. The lactic acid detector and glucose detector are respectively introduced into the glucose content testing chamber and the lactic acid content testing chamber; the sweating measurement component is placed between the first encapsulation layer and the second encapsulation layer, and then the second encapsulation layer portion and the first encapsulation layer portion are encapsulated together using an encapsulating agent; double-sided adhesive tape with a release layer is applied to the outer surface of the second encapsulation layer; sweat inlet holes are formed in the second encapsulation layer and the double-sided adhesive tape, and the contact liquid guiding line extends out of the outer end face of the double-sided adhesive tape through the sweat inlet holes.

[0008] Further, the steps for preparing the first encapsulation layer are as follows: the ratio of the polydimethylsiloxane curing agent to the PDMS basic component solution is 1:1 to 1:20; the oven temperature is 40 to 130 ℃; and the curing time is 0.5 to 6 hours.

[0009] Furthermore, the magnetic gold particles Fe3O4 / DEX / PDA@Au (Raman reporters)@Au are composed of a solution of: ferrous chloride tetrahydrate, ferric chloride hexahydrate, dextran, N-diglycine buffer solution, dopamine, chloroauric acid, hydroxylamine hydrochloride, hexadecyltrimethylammonium bromide, polyvinylpyrrolidone, silver nitrate, ascorbic acid, and NaOH, each with a concentration of 1-500 mmol / L.

[0010] Furthermore, the magnetic gold particles Fe3O4 / DEX / PDA@Au(Raman reporters)@Au-MPBA are composed of a solution of: 4-mercaptophenylboronic acid, ferrous chloride tetrahydrate, ferric chloride hexahydrate, dextran, N-diglycine buffer solution, dopamine, chloroauric acid, hydroxylamine hydrochloride, hexadecyltrimethylammonium bromide, polyvinylpyrrolidone, silver nitrate, ascorbic acid, and NaOH, all with a concentration of 1-500 mmol / L.

[0011] Furthermore, the steps for preparing the sweat volume measurement component specifically include: preparing a sweat volume detection solution using a solution of 0.5–50 mmol / L 2,4,6-tripyridyltriazine, methanol, Tween 80, HgSO4, and FeSO4; and processing 18 μL of the sweat detection solution onto multiple solvent carriers, with 2 μL on each solvent carrier.

[0012] Furthermore, the encapsulant is a polydimethylsiloxane curing agent and a PDMS basic component solution, and the ratio of the two is 1:1 to 1:20. The temperature of the oven is 40 to 130 ℃, and the curing time of the mixture in the oven is 0.5 to 6 hours.

[0013] Furthermore, the amount of Fe3O4 / DEX / PDA@Au (Raman reporters)@Au magnetic gold particles used is 20 μL.

[0014] Furthermore, the amount of Fe3O4 / DEX / PDA@Au(Raman reporters)@Au-MPBA magnetic gold particles used is 20 μL.

[0015] Furthermore, the mold is made of copper.

[0016] Furthermore, the solvent carrier is a circular fabric, and there are nine pieces arranged in a ring; the connecting liquid guiding line and the contact liquid guiding line are both yarns.

[0017] The present invention also provides a multimode sensor for monitoring sweat biomarkers, wherein the multimode sensor is prepared by the aforementioned method for preparing a multimode sensor for monitoring sweat biomarkers.

[0018] Compared with the prior art, the method for preparing a multi-mode sensor for monitoring sweat biomarkers and the multi-mode sensor device provided by the present invention are easy to manufacture. The multi-mode sensor device for monitoring sweat biomarkers is easy to manufacture, and the prepared multi-mode sensor has high reliability and sensitivity. It shows important potential in biosensors and medical research. These advances promote the practical application of sweat sensing platforms. Attached Figure Description

[0019] Figure 1 This is an exploded view and a finished product assembly diagram of the multi-mode sensor device prepared according to the method for preparing a multi-mode sensor for monitoring sweat biomarkers in this application.

[0020] Figure 2 This is a top-view schematic diagram of a multimode sensor device for monitoring sweat biomarkers according to this application, including dimension markings.

[0021] Figure 3This application relates to a multi-mode sensor for monitoring sweat biomarkers. Figure 2 A three-dimensional perspective diagram with dimension annotations.

[0022] Figure 4 This is a schematic diagram of a multi-mode sensor device for monitoring sweat biomarkers applied according to this application when connected to a user's arm.

[0023] Figure 5 This is a schematic diagram of a multi-mode sensor device for monitoring sweat biomarkers applied to the user's head according to this application.

[0024] Figure 6 This application discloses a multi-mode sensor for monitoring sweat biomarkers, which collects SERS spectra of FDPA(MBA)A, FDPA(4-Mpy)A, FDPA(MMTA)A, FDPA(DTNB)A, and FDPPA.

[0025] Figure 7 This application discloses a multimode sensor for monitoring sweat biomarkers, which collects Raman signal intensities of FDPA(MBA)A-MPBA, FDPA(4-Mpy)A-MPBA, FDPA(MMTA)A-MPBA, FDPA(DTNB)A-MPBA, and FDPPA-MPBA.

[0026] Figure 8 This application presents a schematic diagram illustrating the proposed mechanism of the reaction between fe3o4 / DEX / PDA@gold(DTNB)@gold and lactic acid, and the reaction between fe3o4 / DEX / PDA@gold(DTNB)@gold-MPBA and glucose in the data collected by a multi-mode sensor for monitoring sweat biomarkers.

[0027] Figure 9 This application discloses a multi-mode sensor for monitoring sweat biomarkers, which collects SERS spectra of FDPA(DTNB)A with different lactic acid concentrations (0 mM-30 mM).

[0028] Figure 10 This application discloses a fitting function for determining the concentration of lactic acid in sweat based on the SERS peaks located at 1082 and 1334 cm⁻¹ in the data collected by a multimodal sensor for monitoring sweat biomarkers.

[0029] Figure 11 The data acquired by this application using a multi-mode sensor for monitoring sweat biomarkers includes typical SERS spectra of FDPPA-MPBA with different glucose concentrations.

[0030] Figure 12This application discloses a multimode sensor for monitoring sweat biomarkers, in which the acquired data is the SERS intensity ratio (I1574 / I1332) as a power function of glucose concentration. The error bars represent the relative standard deviation of three repeated trials.

[0031] The reference numerals in the figure are as follows: 1. First encapsulation layer; 1-1. Glucose content testing chamber; 1-2. Lactic acid content testing chamber; 2. Lactic acid detection element; 3. Glucose detection element; 4. Sweat measurement component; 4-1. Solvent carrier; 4-2. Connecting liquid guide line; 4-3. Contact liquid guide line; 5. Second encapsulation layer; 5-1. Sweat inlet hole; 5-2. Sweat inlet yarn. Detailed Implementation

[0032] Please refer to Figures 1-5 This embodiment provides a method for preparing a multi-mode sensor for monitoring sweat biomarkers, the preparation method including the following steps: To prepare the first encapsulation layer 1, polydimethylsiloxane curing agent and PDMS basic component solution are mixed in a certain proportion, stirred and placed in a mold. The mold is then placed in an oven for curing. After curing, the first encapsulation layer 1 is formed and then set aside. The first encapsulation layer is provided with a glucose content testing chamber 1-1 and a lactic acid content testing chamber 1-2. To prepare lactic acid detection component 2, Fe3O4 / DEX / PDA@Au (Raman reporters)@Au magnetic gold particles were freeze-dried to obtain a corresponding powdered first magnetic gold particle product, which was black in color. To prepare glucose detection component 3, Fe3O4 / DEX / PDA@Au(Raman reporters)@Au-MPBA magnetic gold particles were freeze-dried to obtain a corresponding powdered second magnetic gold particle product, which was black in color. The magnetic gold particles used as lactic acid and glucose detection reagents were developed by the inventors. The specific preparation process has been published in the journal *Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy*, with the title "Development of Fe3O4 / DEX / PDA@Au(Raman reporters)@Au-MPBA nanocomposites based multi-hotspot SERS probe for ultrasensitive, reliable, and quantitative detection of glucose in sweat".

[0033] Prepare a sweat volume measurement component 4, process the sweat detection solution onto the solvent carrier 4-1, arrange the processed solvent carriers 4-1 in a predetermined array, connect the multiple solvent carriers 4-1 in the array in series using connecting liquid guide lines 4-2, and have contact liquid guide lines 4-3 extending outward from the center of one or more solvent carriers 4-1. To prepare the second encapsulation layer 5, take PDMS curing agent and PDMS basic component solution, stir them and pour them into a mold, put them in an oven for curing, and the second encapsulation layer 5 is formed after curing. Preferably, the ratio of PDMS curing agent to PDMS basic component solution in the second encapsulation layer 5 is 1:1 to 1:20, the temperature of the oven is 40 to 130 ℃, and the curing time of the mixture in the oven is 0.5 to 6 hours.

[0034] The lactic acid detector 2 and glucose detector 3 are respectively introduced into the glucose content testing chamber 1-1 and the lactic acid content testing chamber 1-2; the sweating measurement component 4 is placed between the first encapsulation layer 1 and the second encapsulation layer 5, and then the second encapsulation layer 5 and the first encapsulation layer 1 are encapsulated together using an encapsulating agent; double-sided adhesive tape 6 with a release layer is glued to the outer surface of the second encapsulation layer 5; a sweat inlet hole 5-1 is opened in the second encapsulation layer 5 and the double-sided adhesive tape 6, and the contact liquid guiding line 4-3 extends out of the outer end face of the double-sided adhesive tape through the sweat inlet hole.

[0035] After removing the release layer from the finished multimode sensor, the outer end of the double-sided tape 6 can adhere to human skin.

[0036] The contact liquid guiding line 4-3 can be configured as a separate unit or as a single unit. When the contact liquid guiding line 4-3 is configured as a separate unit, the main body of the contact liquid guiding line 4-3 extends outward from the center of the solvent carrier 4-1, while the other part of the contact liquid guiding line 4-3 is a sweat-guiding yarn 5-2 that passes through the second encapsulation layer 5, double-sided adhesive tape 6, and connects to the main body of the contact liquid guiding line 4-3. In this structural configuration, when the connecting liquid guiding line, the contact liquid guiding line, and the sweat-guiding yarn 5-2 are all made of yarn, the yarn will swell after absorbing water, thus sealing the contact port of the yarn entering the device and avoiding the risk of air and leakage.

[0037] Double-sided tape 6 is preferably medical double-sided tape.

[0038] The measurement of sweat volume, along with lactate and glucose detection, is incorporated into this multi-mode sensor. Simultaneous acquisition of these three parameters prevents significant biases in the analysis of sweat glucose and lactate when the sweat loss rate is unclear. Since sweat loss plays a crucial role in maintaining body temperature and electrolyte balance, this improves the accuracy of the test results.

[0039] Further, the specific steps for preparing the first encapsulation layer 1 are as follows: the ratio of the polydimethylsiloxane curing agent to the PDMS basic component solution is 1:1 to 1:20; the oven temperature is 40 to 130 ℃; and the curing time is 0.5 to 6 hours.

[0040] Furthermore, the magnetic gold particles Fe3O4 / DEX / PDA@Au (Raman reporters)@Au are composed of: ferrous chloride tetrahydrate (FeCl2·4H2O), ferric chloride hexahydrate (FeCl3·6H2O), dextran, N-diglycine buffer solution, dopamine (PDA), chloroauric acid (HAuCl4), hydroxylamine hydrochloride (NH2OH·HCl), hexadecyltrimethylammonium bromide (CTAB), polyvinylpyrrolidone (PVP), silver nitrate (AgNO3), ascorbic acid (L-AA), and NaOH, all at a concentration of 1-500 mmol / L.

[0041] Furthermore, the magnetic gold particles Fe3O4 / DEX / PDA@Au (Raman reporters)@Au-MPBA are composed of: 4-mercaptophenylboronic acid (MPBA), ferrous chloride tetrahydrate (FeCl2·4H2O), ferric chloride hexahydrate (FeCl3·6H2O), dextran, N-diglycine buffer solution, dopamine (PDA), chloroauric acid (HAuCl4), hydroxylamine hydrochloride (NH2OH·HCl), hexadecyltrimethylammonium bromide (CTAB), polyvinylpyrrolidone (PVP), silver nitrate (AgNO3), ascorbic acid (L-AA), and NaOH, all at a concentration of 1-500 mmol / L.

[0042] Further, the steps for preparing the sweat volume measurement component 4 specifically include: preparing a sweat volume detection solution using a solution of 0.5-50 mmol / L 2,4,6-tripyridyltriazine (TPTZ), methanol, Tween 80, HgSO4 and FeSO4; and processing 18 μL of the sweat detection solution onto multiple solvent carriers 4-1, with 2 μL on each solvent carrier.

[0043] Furthermore, the encapsulant is a polydimethylsiloxane curing agent and a PDMS basic component solution, and the ratio of the two is 1:1 to 1:20. The temperature of the oven is 40 to 130 ℃, and the curing time of the mixture in the oven is 0.5 to 6 hours.

[0044] Furthermore, the amount of Fe3O4 / DEX / PDA@Au (Raman reporters)@Au magnetic gold particles used is 20 μL.

[0045] Furthermore, the amount of Fe3O4 / DEX / PDA@Au(Raman reporters)@Au-MPBA magnetic gold particles used is 20 μL.

[0046] Furthermore, the mold is made of copper.

[0047] Polydimethylsiloxane, also known as PDMS or dimethylsiloxane, is a polymer used in the manufacture and molding of microfluidic chips. It is a mineral-organic polymer (containing carbon and silicon structures) belonging to the siloxane family. Besides microfluidics, it can also be used as a food additive (E900), and as an antifoaming agent in shampoos, beverages, and lubricants. To manufacture microfluidic devices, PDMS (liquid) is mixed with a crosslinking agent, poured into a microstructure mold, and heated to obtain a flexible PDMS device (PDMS crosslinking).

[0048] The use of copper in the mold makes it easier to create molds for PDMS compared to other materials.

[0049] Furthermore, the solvent carrier 4-1 is a circular fabric, and there are 9 pieces arranged in a ring; the connecting liquid guiding line 4-2 and the contact liquid guiding line 4-3 are both yarns.

[0050] The solvent carrier 4-1 is a circular fabric. Compared to a three-dimensional structure, a circular sheet occupies less space, which is more conducive to the fabrication of smaller devices. Experiments showed that the diffusion of liquid on the fabric is close to circular. Based on the principle of not wasting area, the solvent carrier 4-1 was designed as a circular fabric.

[0051] With the same solution volume, multi-strand yarns (double strands) cause the absorbed liquid to be distributed laterally within it, thereby reducing the longitudinal transport height and affecting the transport speed. Considering the liquid wicking behavior and transfer effect (less liquid retention in the linear microfluidic channel to eliminate evaporation), a single yarn was selected.

[0052] During the implementation, five types of yarns—bamboo viscose fiber, mercerized cotton fiber, cupro fiber, viscose fiber, and modal fiber—were selected as test bundles. Liquids diffused and spread well within the yarn bundles, with more yarns absorbing more liquid. Compared to other yarns, modal exhibited higher liquid distribution efficiency due to its superior hydrophilicity.

[0053] The connecting threads, contact threads, and sweat-introducing yarn 5-2 act as channels for sweat fluid transmission. The sweat volume detection area has only one sweat introducing hole 5-1 to enter the device, ensuring continuous monitoring even with high sweat volumes. As human movement continues, sweat production increases, and the total detectable sweat volume range across the nine detection points is 10-90 µL.

[0054] Please refer to Figures 1-5 The present invention also provides a multimode sensor for monitoring sweat biomarkers. The multimode sensor is prepared by the aforementioned method for preparing a multimode sensor for monitoring sweat biomarkers. It is a highly sensitive and non-invasive multimode sensor for monitoring sweat biomarkers based on Fe3O4 / DEX / PDA@Au (Raman reporter)@Au, i.e. (FDPA (Raman reporter) A) surface enhanced Raman scattering (SERS) and colorimetric technology.

[0055] 5,5'-Dithiobis(2-nitrobenzoic acid) (DTNB) was identified as the optimized Raman reporter in the core-shell structure of FDPAA. To quantitatively analyze lactate and glucose captured from human sweat, magnets were used to enrich the FDPA(DTNB)A-lactate / glucose complex generated in the reaction.

[0056] Please refer to Figure 1 The five main components of the multimode sensor for monitoring sweat biomarkers, from bottom to top, are: a non-skin-irritating medical adhesive layer, i.e., double-sided tape 6; a second encapsulation layer 5; a sweat volume measurement component 4; a glucose detection area and a lactic acid detection area based on magnetic gold nanoparticles for detecting glucose and lactic acid in sweat, i.e., lactic acid detector 2 located in lactic acid content testing chamber 1-2 and glucose detector 3 located in glucose content testing chamber 1-2; and a first encapsulation layer 1.

[0057] A sweat sensing platform based on adhesive threads, fabric, and PDMS can be attached to the skin with double-sided tape 6 and monitor various sweat parameters such as sweat loss rate, glucose, and lactic acid through designed contact lines and channels, thereby achieving sweat capture and transmission.

[0058] The multimodal sensor device for monitoring sweat biomarkers provided by this invention is based on SERS enhancement technology, primarily through an electromagnetic enhancement mechanism. Roughening the metal surface facilitates the excitation of surface plasmon resonance by electromagnetic waves, significantly increasing the electric field strength on the metal surface. This results in strong Raman scattering by molecules near the metal surface due to the greatly enhanced electric field excitation. The electromagnetic enhancement mechanism is a long-range effect, affecting a range of approximately several hundred angstroms (Å) from the metal surface. The enhancement factor for this effect is 10⁴–10⁶, and it is largely independent of the adsorbed molecules.

[0059] The widespread application of gold nanoparticles (AuNPs) in sensing is likely due to their unique optical, chemical, electrical, and catalytic properties at the nanoscale, along with excellent chemical stability and good biocompatibility. Gold nanomaterials, in particular, not only possess the common characteristics of nanomaterials such as surface effects, small size effects, quantum size effects, and macroscopic quantum tunneling effects, but also exhibit unique optical, electrical, and chemical properties. They are simple to synthesize, have high yields, good biocompatibility, and possess advantages such as large optical absorption and scattering cross-sections and extremely high enhancement effects.

[0060] Please refer to Figure 2 and Figure 3 Specific design dimensions of multimode sensor devices used for monitoring sweat biomarkers. Please refer to Figure 4 and Figure 5 A multimodal sensor for monitoring sweat biomarkers can be installed in multiple locations on the human body, such as the head, arm, and wrist. This multimodal sensor monitors sweat biomarkers during human movement. The multimodal sweat sensor provided in this application, when combined with the arm and head, enables the monitoring of sweat volume, glucose, and lactic acid in human sweat.

[0061] The beneficial effects of this invention are: Because the solvent carrier, connecting liquid guide, and sweat induction yarn are made of cotton fabric and yarn, which are readily available and inexpensive, yarn-based microfluidic devices have promising applications as disposable diagnostic devices in the healthcare field. Their low manufacturing cost and ease of use make them suitable for home and personal use. Yarn-based microfluidic devices have the potential to improve quality of life by providing inexpensive, rapid, portable, and reliable diagnostics. PDMS / fabric-based sensing devices, due to their unique characteristics, especially in resource-scarce environments, can be used to produce personal point-of-care diagnostic devices.

[0062] This invention utilizes hydrophilic viscose fibers as microfluidic channels to deliver sweat analytes. The multimodal sensor device prepared by this invention for monitoring sweat biomarkers is easy to manufacture, exhibits high reliability and sensitivity, and shows significant potential in biosensors and medical research. These advancements are expected to promote the practical application of sweat-sensing platforms, indicating broad application prospects and providing research ideas for the widespread application of flexible wearable sensors in human biomarker monitoring and medical health monitoring. This aims to meet people's expectations and needs for portable technology such as wearable sensing devices.

Claims

1. A method for preparing a multi-mode sensor for monitoring sweat biomarkers, characterized in that, The preparation method includes the following steps: To prepare the first encapsulation layer, polydimethylsiloxane curing agent and PDMS basic component solution are mixed in a certain proportion, stirred, and then placed in a mold. The mold is placed in an oven for curing, and the first encapsulation layer is formed after curing. The first encapsulation layer is provided with a glucose content testing chamber and a lactic acid content testing chamber. To prepare a lactic acid detection element, Fe3O4 / DEX / PDA@Au (Raman reporters)@Au magnetic gold particles were freeze-dried to obtain the corresponding powdered first magnetic gold particle product. To prepare a glucose detection device, Fe3O4 / DEX / PDA@Au(Raman reporters)@Au-MPBA magnetic gold particles were freeze-dried to obtain a corresponding powdered second magnetic gold particle product. A sweat volume measurement component is prepared by processing a sweat detection solution onto a solvent carrier, arranging multiple processed solvent carriers in a predetermined array, and connecting liquid guide lines to connect multiple solvent carriers in the array in series. One or more solvent carriers have contact liquid guide lines extending outward from their centers. To prepare the second encapsulation layer, take PDMS curing agent and PDMS basic component solution, stir them and pour them into a mold, put them in an oven for curing, and the second encapsulation layer is formed after curing. The lactic acid detector and glucose detector are respectively introduced into the glucose content testing chamber and the lactic acid content testing chamber; the sweating measurement component is placed between the first encapsulation layer and the second encapsulation layer, and then the second encapsulation layer portion and the first encapsulation layer portion are encapsulated together using an encapsulating agent; double-sided adhesive tape with a release layer is applied to the outer surface of the second encapsulation layer; sweat inlet holes are formed in the second encapsulation layer and the double-sided adhesive tape, and the contact liquid guiding line extends out of the outer end face of the double-sided adhesive tape through the sweat inlet holes.

2. The method for preparing a multi-mode sensor for monitoring sweat biomarkers according to claim 1, characterized in that, The specific steps for preparing the first encapsulation layer are as follows: the ratio of the polydimethylsiloxane curing agent to the PDMS basic component solution is 1:1 to 1:20; the oven temperature is 40 to 130 ℃; and the curing time is 0.5 to 6 hours.

3. The method for preparing a multi-mode sensor for monitoring sweat biomarkers according to claim 1, characterized in that, The magnetic gold particles Fe3O4 / DEX / PDA@Au (Raman reporters)@Au are composed of a solution of ferrous chloride tetrahydrate, ferric chloride hexahydrate, dextran, N-diglycine buffer solution, dopamine, chloroauric acid, hydroxylamine hydrochloride, hexadecyltrimethylammonium bromide, polyvinylpyrrolidone, silver nitrate, ascorbic acid, and NaOH, each with a concentration of 1-500 mmol / L.

4. The method for preparing a multi-mode sensor for monitoring sweat biomarkers according to claim 1, characterized in that, The magnetic gold particles Fe3O4 / DEX / PDA@Au (Raman reporters)@Au-MPBA are composed of a solution of: 4-mercaptophenylboronic acid, ferrous chloride tetrahydrate, ferric chloride hexahydrate, dextran, N-diglycine buffer solution, dopamine, chloroauric acid, hydroxylamine hydrochloride, hexadecyltrimethylammonium bromide, polyvinylpyrrolidone, silver nitrate, ascorbic acid, and NaOH, all at a concentration of 1-500 mmol / L.

5. The method for preparing a multi-mode sensor for monitoring sweat biomarkers according to claim 1, characterized in that, The steps for preparing the sweat volume measurement component specifically include: preparing a sweat volume detection solution using a solution of 0.5–50 mmol / L 2,4,6-tripyridyltriazine, methanol, Tween 80, HgSO4, and FeSO4; and applying 18 μL of the sweat detection solution to multiple solvent carriers, with 2 μL on each solvent carrier.

6. The method for preparing a multi-mode sensor for monitoring sweat biomarkers according to claim 1, characterized in that, The encapsulant is a polydimethylsiloxane curing agent and a PDMS basic component solution, with a ratio of 1:1 to 1:

20. The oven temperature is 40 to 130 ℃, and the curing time of the mixture in the oven is 0.5 to 6 hours.

7. The method for preparing a multi-mode sensor for monitoring sweat biomarkers according to claim 1, characterized in that, The amount of Fe3O4 / DEX / PDA@Au(Raman reporters)@Au magnetic gold particles and Fe3O4 / DEX / PDA@Au(Ramanreporters)@Au-MPBA magnetic gold particles used is 20 μL.

8. The method for preparing a multi-mode sensor for monitoring sweat biomarkers according to claim 1, characterized in that, The mold is made of copper.

9. The method for preparing a multi-mode sensor for monitoring sweat biomarkers according to claim 1, characterized in that, The solvent carrier is a circular fabric, and there are 9 pieces arranged in a ring; the connecting liquid guiding line and the contact liquid guiding line are both yarns.

10. A multimodal sensor for monitoring sweat biomarkers, characterized in that, The multimode sensor is prepared using the method for preparing a multimode sensor for monitoring sweat biomarkers as described in any one of claims 1 to 9.

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