Multi-index miscible urinalysis particle for pet urine and preparation process of multi-index miscible urinalysis particle

Abstract

The invention relates to the technical field of pet health detection, in particular to a pet urine multi-index mixable urinalysis particle and a preparation technology thereof, multiple indexes can be detected at the same time, and synchronous detection of the multiple indexes of urine is achieved; the method is suitable for various pet animals and has a wide application range; by adopting the technical scheme of mixing the color developing agent or a novel color developing agent, the color distinguishing is obvious; an antioxidant, a biocompatible material PEG and a wrapping material are adopted, so that the stability is good; the stability (more than 30 days after uncovering) of the detected particles, the color development accuracy (the color difference delta E is more than 5) and the multi-species applicability (the dog / cat urine osmotic pressure compatible range is 1.000-1.060) can be realized. The occult blood particles adopt a PEG (Polyethylene Glycol) embedding technology, and the color development can last for more than 15 days. Therefore, the problem that an existing pet urine urinalysis particle can only detect a single index and cannot synchronously detect multiple indexes is solved.

Description

Technical Field

[0001] This invention relates to the field of pet health testing technology, and in particular to a multi-index mixable urine test particle for pet urine and its preparation process. Background Technology

[0002] Currently, pet urine testing products have several shortcomings. Existing urine testing granules typically only detect a single indicator or are only applicable to specific animals. Furthermore, for indicators with similar colors, such as bilirubin and urobilinogen, or glucose and ketone bodies, which are both reddish-brown, it is difficult to distinguish them with the naked eye, making identification challenging when using granules for two to ten different indicators. In addition, existing chromogenic granules are prone to oxidation and fading upon exposure to air, failing to maintain stable color over long periods. For example, occult blood granules currently on the market will gradually fade within 3 days, affecting the interpretation of test results. When multiple indicators are tested together, there is also the problem of cross-interference; for example, protein detection is affected by the pH of the chromogenic reagent.

[0003] Therefore, developing a urine testing product that can simultaneously detect multiple indicators, is applicable to various animals, has clear color differentiation, and good stability is an urgent problem to be solved in the field of pet health testing. Summary of the Invention

[0004] The purpose of this invention is to provide a multi-indicator mixable urine test granule for pet urine and its preparation process, aiming to solve the problem that existing pet urine test granules can only detect a single indicator and cannot detect multiple indicators simultaneously.

[0005] To achieve the above objectives, in a first aspect, the present invention provides a process for preparing multi-index mixable urine test particles from pet urine, comprising the following steps:

[0006] Using the masterbatch as a carrier, the substrate color development layer solution and the masterbatch are mixed at a preset weight ratio, stirred evenly, and then allowed to stand. Then, a drying process is performed to obtain semi-finished product one.

[0007] After spraying an embedding agent solution onto the surface of semi-finished product one, a secondary drying process is performed to obtain semi-finished product two.

[0008] After spraying a coating agent solution onto the surface of the semi-finished product, it undergoes three drying processes to obtain urine test particles.

[0009] In the section "using masterbatch as a carrier, mixing the substrate color development layer solution with masterbatch at a preset weight ratio, stirring evenly and then allowing it to stand, followed by a drying process to obtain semi-finished product one", the masterbatch is a fiber material, bentonite material, or silica gel material; the length of the fiber material, bentonite material, and silica gel material fibers is 1-25mm; the fiber material is selected from one or more of paper fiber, bamboo fiber, coconut shell fiber, flax fiber, cotton fiber, sisal fiber, polyester fiber, nylon fiber, and polyacrylonitrile fiber.

[0010] In the section "using masterbatch as a carrier, the substrate chromogenic layer solution is mixed with masterbatch at a preset weight ratio, stirred evenly and allowed to stand, and then dried once to obtain semi-finished product one", the substrate chromogenic layer solution includes a reaction substrate, a chromogenic agent, a promoter, a free radical scavenger, a metal chelating agent and a buffer solution.

[0011] In the section "after spraying an embedding agent solution onto the surface of semi-finished product one, a secondary drying process is performed to obtain semi-finished product two", the embedding agent solution is polyethylene glycol (PEG-6000 / 8000).

[0012] In the section "after spraying a coating agent solution onto the surface of the semi-finished product and then performing three drying processes to obtain urine test particles", the coating agent is chitosan and hydroxypropyl methylcellulose.

[0013] The temperatures for the primary drying process, the secondary drying process, and the tertiary drying process are 35-65℃.

[0014] Secondly, a multi-index mixable urine test particle for pet urine is prepared using the multi-index mixable urine test particle preparation process described in the first aspect. It includes an inner reaction layer, a middle polyethylene glycol (PEG-6000 / 8000) antioxidant layer, and an outer hydroxypropyl methylcellulose moisture-proof film. The outer hydroxypropyl methylcellulose moisture-proof film also contains titanium dioxide ultraviolet shielding agent, and has a light transmittance of <5%.

[0015] The present invention discloses a process for preparing multi-index mixable urine test particles for pet urine, comprising the following steps: using masterbatch as a carrier, mixing the substrate chromogenic layer solution and masterbatch at a preset weight ratio, stirring evenly and allowing to stand, and then performing a first drying treatment to obtain semi-finished product one; spraying an embedding agent solution on the surface of semi-finished product one and performing a second drying treatment to obtain semi-finished product two; spraying an encapsulating agent solution on the surface of semi-finished product two and performing a third drying treatment to obtain urine test particles. This invention can simultaneously detect multiple indicators, such as occult blood, urobilinogen, bilirubin, glucose, ketone bodies, protein, pH value, nitrite, leukocyte lipase, and nine other indicators, achieving simultaneous detection of multiple indicators in urine. It is suitable for various pet animals, such as dogs and cats, and has a wide range of applications. Employing a mixed or novel chromogenic reagent technology, it solves the problem of visually distinguishing indicators with similar colors (such as bilirubin and urobilinogen, nitrite, glucose and ketone bodies), resulting in clear color differentiation. Using antioxidants, biocompatible PEG, and encapsulation materials, it solves the problem of easy oxidation and fading of existing chromogenic particles after exposure to air; for example, occult blood particles do not require frequent replacement and have good stability. It achieves stable detection particles (>30 days after opening), accurate color development (color difference ΔE>5), and multi-species applicability (dog / cat urine osmolarity compatibility range 1.005-1.060). The occult blood particles utilize PEG encapsulation technology, which solves the fading problem, and the color development can last for more than 15 days. This solves the problem that existing pet urine test particles can only detect a single indicator and cannot detect multiple indicators simultaneously. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 This is a flowchart of a process for preparing multi-index mixable urine test particles for pet urine, provided by the present invention.

[0018] Figure 2 This is a color chart showing the reaction of 1.18% p-aminophenylarsonic acid.

[0019] Figure 3 It is a reaction color diagram of indophenol esters and their derivatives or pyrrolidine esters.

[0020] Figure 4 This is the reaction color diagram of Gulan B salt.

[0021] Figure 5The reaction color diagram is for 3,5-dichlorophenyldiazotetrafluoroborate or 2,4-dichloroaniline diazonium salt.

[0022] Figure 6 This is a color chart of the reaction of sodium 3-nitrophenolate.

[0023] Figure 7 This is a color chart of glucose oxidase and peroxidase reactions.

[0024] Figure 8 The color chart shows the reaction of tetrabromophenol blue or biuret reagent.

[0025] Figure 9 This is a color diagram of the reaction between methyl red and bromophenol blue.

[0026] Figure 10 This is a color chart of the reaction of tetramethylbenzidine.

[0027] Figure 11 It is bromothymol blue; the reaction color diagram of sodium polymethyl methacrylate maleate. Detailed Implementation

[0028] Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the present invention, and should not be construed as limiting the present invention.

[0029] Please see Figures 1 to 11 In a first aspect, the present invention provides a process for preparing multi-index mixable urine test particles from pet urine, comprising the following steps:

[0030] S1 uses masterbatch as a carrier, mixes the substrate color development layer solution with masterbatch at a preset weight ratio, stirs evenly and then stands, and then performs a drying process to obtain semi-finished product one;

[0031] The masterbatch is a fiber material, bentonite material, or silica gel material; the length of the fiber material, bentonite material, and silica gel material fibers is 1-25 mm; the fiber material is selected from one or more of paper fiber, bamboo fiber, coconut shell fiber, flax fiber, cotton fiber, sisal fiber, polyester fiber, nylon fiber, and polyacrylonitrile fiber.

[0032] The substrate chromogenic layer solution includes a reaction substrate, a chromogenic agent, a promoter, a free radical scavenger, a metal chelating agent, and a buffer solution.

[0033] The temperature for the first drying process is 35-65℃.

[0034] Specifically, using masterbatch as a carrier (size: 1-25mm), the substrate color development solution and masterbatch are mixed at a weight ratio of 1:5-2:1. While stirring the particles, a peristaltic pump is used to pressurize the solution and spray it onto the particles until the reagent solution is completely absorbed. Stirring continues until the solution is uniformly absorbed by the blank particles and no white particles are visible. Stirring is then stopped, and the particles are allowed to stand for 1-15 minutes. Then, they are dried once at 35-65℃ until the moisture content is below 10%.

[0035] S2 is subjected to a secondary drying process after spraying an embedding agent solution onto the surface of semi-finished product one to obtain semi-finished product two.

[0036] The encapsulating agent solution is polyethylene glycol (PEG-6000 / 8000).

[0037] The temperature for the secondary drying process is 35-65℃.

[0038] Specifically, it is obtained by spraying an embedding agent solution onto the surface and then drying it twice at 35-65℃ until the moisture content is below 5%.

[0039] S3 is dried three times after spraying a coating agent solution onto the surface of the semi-finished product to obtain urine test particles.

[0040] The encapsulating agent is chitosan and hydroxypropyl methylcellulose.

[0041] The temperature for the three drying processes is 35-65℃.

[0042] Specifically, the surface is then sprayed with a coating agent solution and dried a second time at 35-65°C until the moisture content is below 5%, thus obtaining urine test particles.

[0043] I. Colorimetric Reaction Materials

[0044] 1.1 A technical solution employing mixed or novel colorimetric reagents is proposed. By using colorimetric reagents of different colors or composite colorimetric reagents, and methods such as colorimetric reagent improvement and color difference enhancement agents, the colorimetric reactions of different indicators produce distinctly different colors. This solves the problem of difficulty in visually distinguishing indicators with similar colors (such as bilirubin and urobilinogen, nitrite and microalbumin, glucose and ketone bodies, etc.). Specifically:

[0045] 1.2 Color developer substrate

[0046] Enzymes: Glucose oxidase (GOD) and peroxidase (POD) are used for glucose detection; others.

[0047] 1.3 Color difference enhancer

[0048] Potassium bromide: Used in the bilirubin colorimetric reaction to enhance the contrast between the yellow color and the orange-red color of urobilinogen.

[0049] Titanium dioxide (TiO2): As a white background layer, it improves the color difference recognition of the color particles (ΔE>5);

[0050] Stable reactive materials

[0051] 2.1 Stability Enhancement Technology Solution

[0052] Multi-layer coating structure: inner reactive layer + middle PEG-8000 antioxidant layer + outer hydroxypropyl methylcellulose moisture-proof film

[0053] Light stability: Titanium dioxide UV shielding agent added (transmittance <5%)

[0054] 2.2 Antioxidant System

[0055] Encapsulating agent: Polyethylene glycol (PEG-6000 / 8000) forms a hydrophobic barrier, preventing oxygen penetration.

[0056] Free radical scavengers: Vitamin E (tocopherol), ascorbic acid (vitamin C), potassium iodate, ascorbic acid oxidase, piperidine oxygen free radicals, potassium iodide, etc.

[0057] Metal chelating agent: EDTA-2Na prevents the catalytic oxidation of metal ions;

[0058] 2.3 Moisture-proof and degradation-resistant materials

[0059] Film-forming materials: Hydroxypropyl methylcellulose (HPMC), chitosan (pH-responsive)

[0060] Drying effect: Sodium silicate, magnesium sulfate, etc.

[0061] III. Timing Control Materials

[0062] Delayed release layer: Sodium alginate-calcium ion cross-linked gel, controlling the delayed release of glucose chromogenic agent by 5-10 seconds.

[0063] IV. Special Functional Additives

[0064] 4.1 Light-Stable Materials

[0065] UV absorbers: titanium dioxide, benzotriazole compounds

[0066] 4.2 Multi-species adaptants

[0067] Osmotic pressure regulator: Sodium chloride (suitable for highly concentrated cat urine)

[0068] Anti-crystallization agent: Sodium citrate (prevents interference from phosphate crystallization in dog urine)

[0069] 4.3 Example of Material Combination (Occult Blood Detection Particles)

[0070] Core layer: TMB + peroxide (colorimetric reaction matrix)

[0071] Intermediate layer: Vitamin E encapsulated in PEG-6000 (antioxidant layer)

[0072] Outer layer: Chitosan / HPMC composite membrane (disintegrates above pH 5.0)

[0073] The above material system can achieve the stability of the detected particles (>30 days after opening), the accuracy of color development (color difference ΔE>5) and the applicability to multiple species (compatible range of osmolarity for dog / cat urine 1.005-1.060).

[0074] Enhanced color stability: The occult blood granules utilize PEG encapsulation technology, which solves the fading problem, ensuring color development for over 15 days. See the table below for details.

[0075]

[0076]

[0077] Example 1:

[0078] A mixable pet urine test particle that enables simultaneous detection of multiple indicators in urine using a composite colorimetric reagent includes the following steps:

[0079] Using masterbatch as a carrier (size: 1-25mm), the substrate chromogenic layer solution and masterbatch are mixed at a weight ratio of 1:5-2:1. While stirring the particles, a peristaltic pump pressurizes the solution and sprays it onto the particles through a special nozzle until the reagent solution is completely absorbed. Stirring continues until homogeneous, the blank particles have uniformly absorbed the reagent solution, and no white particles remain. Stirring is then stopped, and the mixture is allowed to stand for 1-15 minutes. It is then dried once at 35-65℃ until the moisture content is below 10%. After spraying an embedding agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%. Finally, after spraying an encapsulating agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%.

[0080] The masterbatch includes fiber materials selected from one or more of paper fiber, bamboo fiber, coconut shell fiber, flax fiber, cotton fiber, sisal fiber, polyester fiber, nylon fiber, and polyacrylonitrile fiber; the length of the fiber is controlled between 1-25 mm.

[0081] The masterbatch includes bentonite material with a length controlled between 1-25 mm.

[0082] The masterbatch comprises silicone material and has a length controlled between 1-25 mm.

[0083] Instructions for use: Mix one or more test pellets with cat litter at a weight ratio of 1:300 and use in the litter box; or (a) remove the urine test pellets from the packaging bag; (b) pour pet urine onto the urine test pellets; (c) after 3 seconds, compare the reaction time of different items with the color mark, find the color mark with the closest color, and the value marked on the color mark is the test result; or use it in conjunction with a suitable testing instrument after testing.

[0084] 1. The composition of the inner layer glucose substrate colorimetric solution of [glucose granules] is as follows:

[0085] Reaction substrates: glucose oxidase 10-35 kDa / L, peroxidase 5-50 kDa / L.

[0086] Colorimetric reagent: p, p-tetramethyl-diamino-diphenylmethane 0.1-15 wt%

[0087] Accelerator: Glutaraldehyde 0.1-15wt%;

[0088] Free radical scavengers: one or more of the following: vitamin E (tocopherol), ascorbic acid (vitamin C), potassium iodate, ascorbic acid oxidase, piperidine oxygen free radicals, potassium iodide, etc.; 0.1-15wt%;

[0089] Metal chelating agents: EDTA.2Na, EDTA.4Na, EDTA, or one or more of the following;

[0090] Sustained-release agent: 0.05%-1% sodium alginate or β-cyclodextrin, or a mixture of two of them;

[0091] Buffer solution: Balance. The pH of the buffer solution is 6.0-8.5, and the buffer solution is selected from one of the following: 0.01-0.5 mol / L Tris-HCl buffer, citrate buffer, HEPES buffer, and borate buffer. Citrate buffer solution is preferred to prevent interference from phosphate crystallization in canine urine.

[0092] The intermediate layer embedding agent is: polyethylene glycol (PEG-6000 / 8000) with a mass fraction of 1%-5% to form a hydrophobic barrier and block oxygen penetration;

[0093] The outer coating agent is: 1%-5% by mass of chitosan (pH-responsive) / sodium carboxypropyl cellulose, 1%-5% titanium dioxide ultraviolet shielding agent, a small amount of silica, calcium chloride, magnesium sulfate and other moisture-proof substances, or a small amount of adhesive gelatin and polyvinyl butyral.

[0094] The preparation method of glucose detection particles includes the following steps:

[0095] Using a masterbatch of fiber material as a carrier, the substrate colorimetric layer solution and the masterbatch are mixed at a weight ratio of 1:5-2:1. While stirring the particles, a peristaltic pump pressurizes the solution and sprays it onto the particles through a specially designed nozzle until the reagent solution is completely absorbed. Stirring continues until the mixture is homogeneous, the blank particles have uniformly absorbed the reagent solution, and no white particles remain. Stirring is then stopped, and the mixture is allowed to stand for 1-15 minutes. It is then dried once at 35-65℃ until the moisture content is below 10%. After spraying an embedding agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%. Finally, after spraying an encapsulating agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%.

[0096] 2. The composition of the occult blood substrate colorimetric solution in the inner layer of [occult blood granules] is as follows:

[0097] Reaction substrate: 0.05-0.2 wt% of cumene peroxide and its derivatives.

[0098] Colorimetric reagent: Tetramethylbenzidine 0.05-0.2 wt%

[0099] Accelerator: 0.1-15 wt% of 4-methoxyquinoline derivatives;

[0100] Metal chelating agents: EDTA.2Na, EDTA.4Na, EDTA, or one or more of the following;

[0101] Fading retardants: Sodium diisooctyl sulfosuccinate, phenylaminourea compounds, disodium glycerol phosphate hydrate, or a mixture of several of these.

[0102] Osmotic pressure regulator (promotes cell lysis): Sodium chloride;

[0103] Buffer solution: Balance. The pH of the buffer solution is 6.0-9.0, and the pH of the buffer solution is 6.0-8.5. The buffer solution is selected from one of the following buffer solutions: 0.01-0.5 mol / L Tris-HCl buffer, citrate buffer, HEPES buffer, borate buffer, borate-trisodium citrate buffer, etc.

[0104] The intermediate layer embedding agent is: 1%-5% polyethylene glycol (PEG-6000 / 8000) to form a hydrophobic barrier and block oxygen penetration; the color fixing agent is: 1%-5% of quaternary ammonium salt cationic surfactant or polyvinylpyrrolidone K30, K90 cationic surfactant or a mixture of multiple types.

[0105] The outer coating agent is: 1%-5% by mass of chitosan (pH-responsive) / sodium carboxypropyl cellulose, 1%-5% titanium dioxide ultraviolet shielding agent, a small amount of silica, calcium chloride, magnesium sulfate and other moisture-proof substances, or a small amount of adhesive gelatin and polyvinyl butyral.

[0106] The preparation method of occult blood detection particles includes the following steps:

[0107] Using a masterbatch of fiber material as a carrier, the substrate colorimetric layer solution and the masterbatch are mixed at a weight ratio of 1:5-2:1. While stirring the particles, a peristaltic pump pressurizes the solution and sprays it onto the particles through a specially designed nozzle until the reagent solution is completely absorbed. Stirring continues until the mixture is homogeneous, the blank particles have uniformly absorbed the reagent solution, and no white particles remain. Stirring is then stopped, and the mixture is allowed to stand for 1-15 minutes. It is then dried once at 35-65℃ until the moisture content is below 10%. After spraying an embedding agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%. Finally, after spraying an encapsulating agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%.

[0108] 3. The composition of the inner leukocyte substrate colorimetric solution [leukocyte granules] is as follows:

[0109] Reaction substrate: 0.05-0.2 wt% of indophenol esters and their derivatives or pyrrolidine esters and their derivatives.

[0110] Colorimetric reagent: 0.05-0.2 wt% of 2-methoxy-4-(N-morpholino)-benzenediazozinc tetrachloride salt

[0111] Accelerator: 0.1-15 wt% of sebac glycol derivatives;

[0112] Metal chelating agents: EDTA.2Na, EDTA.4Na, EDTA, or one or more of the following;

[0113] Osmotic pressure regulator (promotes cell lysis): Sodium chloride;

[0114] Buffer solution: Balance. The pH of the buffer solution is 4.0-7.0, and the pH of the buffer solution is 6.0-8.5. The buffer solution is selected from one of the following buffer solutions: 0.01-0.5 mol / L Tris-HCl buffer, citrate buffer, HEPES buffer, borate buffer, borate-trisodium citrate buffer, etc. A citrate buffer system is preferred.

[0115] The intermediate layer embedding agent is: polyethylene glycol (PEG-6000 / 8000) with a mass fraction of 1%-5% to form a hydrophobic barrier and block oxygen penetration;

[0116] The outer coating agent is: 1%-5% by mass of chitosan (pH-responsive) / sodium carboxypropyl cellulose, 1%-5% titanium dioxide ultraviolet shielding agent, a small amount of silica, calcium chloride, magnesium sulfate and other moisture-proof substances, or a small amount of adhesive gelatin and polyvinyl butyral.

[0117] The method for preparing white blood cell detection particles includes the following steps:

[0118] Using a masterbatch of fiber material as a carrier, the substrate colorimetric layer solution and the masterbatch are mixed at a weight ratio of 1:5-2:1. While stirring the particles, a peristaltic pump pressurizes the solution and sprays it onto the particles through a specially designed nozzle until the reagent solution is completely absorbed. Stirring continues until the mixture is homogeneous, the blank particles have uniformly absorbed the reagent solution, and no white particles remain. Stirring is then stopped, and the mixture is allowed to stand for 1-15 minutes. It is then dried once at 35-65℃ until the moisture content is below 10%. After spraying an embedding agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%. Finally, after spraying an encapsulating agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%.

[0119] 4. The composition of the inner layer nitrite substrate colorimetric solution [nitrite particles] is as follows:

[0120] Reaction substrate: p-Aminophenylarsonic acid or p-Aminophenylsulfonic acid 0.05-0.2 wt%.

[0121] Colorimetric reagent: 0.05-0.2 wt% of 3-hydroxy-1,2,3,4-tetrahydrobenzoquinoline

[0122] Metal chelating agents: EDTA.2Na, EDTA.4Na, EDTA, or one or more of the following;

[0123] Free radical scavengers: one or more of the following: vitamin E (tocopherol), ascorbic acid (vitamin C), potassium iodate, ascorbic acid oxidase, piperidine oxygen free radicals, potassium iodide, etc.; 0.1-15wt%;

[0124] Buffer solution: Balance. The buffer solution has a pH of 1.0-2.0 and contains one or more of the following: boric acid, citric acid, phosphoric acid, metaphosphoric acid, sulfosalicylic acid, hydrochloric acid, and sulfuric acid, at a concentration of 0.01-0.5 mol / L.

[0125] The intermediate layer embedding agent is: 1%-5% polyethylene glycol (PEG-6000 / 8000) to form a hydrophobic barrier and block oxygen penetration; the color fixing agent is: 1%-5% of quaternary ammonium salt cationic surfactant or polyvinylpyrrolidone K30, K90 cationic surfactant or a mixture of multiple types.

[0126] The outer coating agent is: 1%-5% by mass of chitosan (pH-responsive) / sodium carboxypropyl cellulose, 1%-5% titanium dioxide ultraviolet shielding agent, a small amount of silica, calcium chloride, magnesium sulfate and other moisture-proof substances, or a small amount of adhesive gelatin and polyvinyl butyral.

[0127] The preparation method of nitrite detection particles includes the following steps:

[0128] Using a masterbatch of fiber material as a carrier, the substrate colorimetric layer solution and the masterbatch are mixed at a weight ratio of 1:5-2:1. While stirring the particles, a peristaltic pump pressurizes the solution and sprays it onto the particles through a specially designed nozzle until the reagent solution is completely absorbed. Stirring continues until the mixture is homogeneous, the blank particles have uniformly absorbed the reagent solution, and no white particles remain. Stirring is then stopped, and the mixture is allowed to stand for 1-15 minutes. It is then dried once at 35-65℃ until the moisture content is below 10%. After spraying an embedding agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%. Finally, after spraying an encapsulating agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%.

[0129] 5. The composition of the urobilinogen substrate colorimetric solution in the inner layer of [urobilinogen granules] is as follows:

[0130] Reaction substrate 1: 0.05%-0.2wt% of Gulan B salt

[0131] Reaction substrate 2: one or more of the following: Solid Red GBC, Solid Red B, Solid Red-Naphthalenesulfonic Acid TR, and Solid Red Violet LB salt, in a concentration of 0.01-0.05 wt%

[0132] Metal chelating agents: EDTA.2Na, EDTA.4Na, EDTA, or one or more of the following;

[0133] Free radical scavengers: one or more of the following, in combination: vitamin E (tocopherol), ascorbic acid (vitamin C), potassium iodate, ascorbic acid oxidase, piperidine oxygen free radicals, potassium iodide, etc.; 0.1-15 wt%;

[0134] Buffer solution: Balance. The buffer solution has a pH of 1.0-2.0 and contains one of the following at a concentration of 0.01-0.5 mol / L: boric acid, citric acid, phosphoric acid, metaphosphoric acid, sulfosalicylic acid, hydrochloric acid, or sulfuric acid.

[0135] The intermediate layer embedding agent is: 1%-5% polyethylene glycol (PEG-6000 / 8000) to form a hydrophobic barrier and block oxygen penetration; the color fixing agent is: 1%-5% of quaternary ammonium salt cationic surfactant or polyvinylpyrrolidone K30, K90 cationic surfactant or a mixture of multiple types.

[0136] The outer coating agent is: 1%-5% by mass of chitosan (pH-responsive) / sodium carboxypropyl cellulose, 1%-5% titanium dioxide ultraviolet shielding agent, a small amount of silica, calcium chloride, magnesium sulfate and other moisture-proof substances, or a small amount of adhesive gelatin and polyvinyl butyral.

[0137] The preparation method of urobilinogen detection particles includes the following steps:

[0138] Using a masterbatch of fiber material as a carrier, the substrate colorimetric layer solution and the masterbatch are mixed at a weight ratio of 1:5-2:1. While stirring the particles, a peristaltic pump pressurizes the solution and sprays it onto the particles through a specially designed nozzle until the reagent solution is completely absorbed. Stirring continues until the mixture is homogeneous, the blank particles have uniformly absorbed the reagent solution, and no white particles remain. Stirring is then stopped, and the mixture is allowed to stand for 1-15 minutes. It is then dried once at 35-65℃ until the moisture content is below 10%. After spraying an embedding agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%. Finally, after spraying an encapsulating agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%.

[0139] 6. The composition of the inner bilirubin substrate colorimetric solution of the [bilirubin granules] is as follows:

[0140] Reaction substrate: 0.05%-0.2wt% of 3,5-dichlorophenyldiazotetrafluoroborate or 2,4-dichloroaniline diazonium salt;

[0141] Colorimetric enhancer: Potassium bromide 0.01-0.05 wt%;

[0142] Metal chelating agents: EDTA.2Na, EDTA.4Na, EDTA, or one or more of the following;

[0143] Free radical scavengers: one or more of the following, in combination: vitamin E (tocopherol), ascorbic acid (vitamin C), potassium iodate, ascorbic acid oxidase, piperidine oxygen free radicals, potassium iodide, etc.; 0.1-15 wt%;

[0144] Buffer solution: Balance. The buffer solution has a pH of 1.0-2.0 and contains one of the following at a concentration of 0.01-0.5 mol / L: boric acid, citric acid, phosphoric acid, metaphosphoric acid, sulfosalicylic acid, hydrochloric acid, or sulfuric acid.

[0145] The intermediate layer embedding agent is: 1%-5% polyethylene glycol (PEG-6000 / 8000) to form a hydrophobic barrier and block oxygen penetration; the color fixing agent is: 1%-5% of quaternary ammonium salt cationic surfactant or polyvinylpyrrolidone K30, K90 cationic surfactant or a mixture of multiple types.

[0146] The outer coating agent is: 1%-5% by mass of chitosan (pH-responsive) / sodium carboxypropyl cellulose, 1%-5% titanium dioxide ultraviolet shielding agent, a small amount of silica, calcium chloride, magnesium sulfate and other moisture-proof substances, or a small amount of adhesive gelatin and polyvinyl butyral.

[0147] The preparation method of bilirubin detection particles includes the following steps:

[0148] Using a masterbatch of fiber material as a carrier, the substrate colorimetric layer solution and the masterbatch are mixed at a weight ratio of 1:5-2:1. While stirring the particles, a peristaltic pump pressurizes the solution and sprays it onto the particles through a specially designed nozzle until the reagent solution is completely absorbed. Stirring continues until the mixture is homogeneous, the blank particles have uniformly absorbed the reagent solution, and no white particles remain. Stirring is then stopped, and the mixture is allowed to stand for 1-15 minutes. It is then dried once at 35-65℃ until the moisture content is below 10%. After spraying an embedding agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%. Finally, after spraying an encapsulating agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%.

[0149] 7. The composition of the inner ketone body substrate colorimetric solution for [ketone body particles] is as follows:

[0150] Reaction substrate: 0.05%-0.2 wt% of sodium 3-nitrophenolate;

[0151] Colorimetric enhancer: glycine 0.01-0.05 wt%;

[0152] Stabilizer: 1%-5% anhydrous magnesium sulfate;

[0153] Buffer solution: Balance. The buffer solution has a pH of 9.0-11.0 and is an alkaline buffer solution such as 0.01-0.5 mol / L Tris or sodium hydroxide.

[0154] The intermediate layer embedding agent is: polyethylene glycol (PEG-6000 / 8000) with a mass fraction of 1%-5% to form a hydrophobic barrier and block oxygen penetration;

[0155] The outer coating agent is: 1%-5% by mass of chitosan (pH-responsive) / sodium carboxypropyl cellulose, 1%-5% titanium dioxide ultraviolet shielding agent, a small amount of silica, calcium chloride, magnesium sulfate and other moisture-proof substances, or a small amount of adhesive gelatin and polyvinyl butyral.

[0156] The preparation method of ketone body detection particles includes the following steps:

[0157] Using a masterbatch of fiber material as a carrier, the substrate colorimetric layer solution and the masterbatch are mixed at a weight ratio of 1:5-2:1. While stirring the particles, a peristaltic pump pressurizes the solution and sprays it onto the particles through a specially designed nozzle until the reagent solution is completely absorbed. Stirring continues until the mixture is homogeneous, the blank particles have uniformly absorbed the reagent solution, and no white particles remain. Stirring is then stopped, and the mixture is allowed to stand for 1-15 minutes. It is then dried once at 35-65℃ until the moisture content is below 10%. After spraying an embedding agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%. Finally, after spraying an encapsulating agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%.

[0158] 8. The composition of the inner layer pH substrate colorimetric solution of [pH particles] is as follows:

[0159] Reaction substrate 1: Methyl red 0.01-0.05 wt%;

[0160] Reaction substrate 2: Bromophenol blue 0.01-0.05 wt%;

[0161] Color enhancement agent: Citric acid 0.001-0.05wt%;

[0162] Solvent: Balance. One or a mixture of methanol, ethanol, and acetone;

[0163] Surfactants: Nonionic surfactants, Tween 20 / 80, and one or more OP emulsifiers in combination;

[0164] Adhesive: 1.0-10.0 wt% of one or more of adhesives, such as gelatin and polyvinyl butyral;

[0165] The intermediate layer embedding agent is: polyethylene glycol (PEG-6000 / 8000) with a mass fraction of 1%-5% to form a hydrophobic barrier and block oxygen penetration;

[0166] The outer coating agent is: 1%-5% by mass of chitosan (pH-responsive) / sodium carboxypropyl cellulose, 1%-5% titanium dioxide ultraviolet shielding agent, a small amount of silica, calcium chloride, magnesium sulfate and other moisture-proof substances, or a small amount of adhesive gelatin and polyvinyl butyral.

[0167] The preparation method of ketone body detection particles includes the following steps:

[0168] Using a masterbatch of fiber material as a carrier, the substrate colorimetric layer solution and the masterbatch are mixed at a weight ratio of 1:5-2:1. While stirring the particles, a peristaltic pump pressurizes the solution and sprays it onto the particles through a specially designed nozzle until the reagent solution is completely absorbed. Stirring continues until the mixture is homogeneous, the blank particles have uniformly absorbed the reagent solution, and no white particles remain. Stirring is then stopped, and the mixture is allowed to stand for 1-15 minutes. It is then dried once at 35-65℃ until the moisture content is below 10%. After spraying an embedding agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%. Finally, after spraying an encapsulating agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%.

[0169] 9. [Specific Gravity Particles] The composition of the inner layer specific gravity substrate colorimetric solution is as follows:

[0170] Reaction substrate: 0.01-0.05 wt% of methoxyethylene maleic acid polymer;

[0171] Color developer: Bromothymol blue 0.001-0.05 wt%;

[0172] Solvent: Balance. One or a mixture of methanol, ethanol, and acetone;

[0173] Surfactants: Nonionic surfactants, Tween 20 / 80, and one or more OP emulsifiers in combination;

[0174] Adhesive: 1.0-10.0 wt% of one or more of adhesives, such as gelatin and polyvinyl butyral;

[0175] The intermediate layer embedding agent is: polyethylene glycol (PEG-6000 / 8000) with a mass fraction of 1%-5% to form a hydrophobic barrier and block oxygen penetration;

[0176] The outer coating agent is: 1%-5% by mass of chitosan (pH-responsive) / sodium carboxypropyl cellulose, 1%-5% titanium dioxide ultraviolet shielding agent, a small amount of silica, calcium chloride, magnesium sulfate and other moisture-proof substances, or a small amount of adhesive gelatin and polyvinyl butyral.

[0177] The preparation method of ketone body detection particles includes the following steps:

[0178] Using a masterbatch of fiber material as a carrier, the substrate colorimetric layer solution and the masterbatch are mixed at a weight ratio of 1:5-2:1. While stirring the particles, a peristaltic pump pressurizes the solution and sprays it onto the particles through a specially designed nozzle until the reagent solution is completely absorbed. Stirring continues until the mixture is homogeneous, the blank particles have uniformly absorbed the reagent solution, and no white particles remain. Stirring is then stopped, and the mixture is allowed to stand for 1-15 minutes. It is then dried once at 35-65℃ until the moisture content is below 10%. After spraying an embedding agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%. Finally, after spraying an encapsulating agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%.

[0179] 10. The composition of the inner layer protein substrate colorimetric solution [protein particles] is as follows:

[0180] Reaction substrate: 0.01-0.05 wt% of either tetrabromophenol blue or biuret reagent;

[0181] Solvent: 5-50 wt% of one or more of methanol, ethanol, and acetone;

[0182] Surfactants: Nonionic surfactants, Tween 20 / 80, and one or more OP emulsifiers in combination;

[0183] Adhesive: One or more of adhesives, such as gelatin and polyvinyl butyral, in a concentration of 1.0%-10.0 wt%;

[0184] Balance. The pH of the buffer solution is 1.0-3.0, and it contains one of the following: boric acid, citric acid, etc., at a concentration of 0.01-0.5 mol / L.

[0185] The intermediate layer embedding agent is: polyethylene glycol (PEG-6000 / 8000) with a mass fraction of 1%-5% to form a hydrophobic barrier and block oxygen penetration;

[0186] The outer coating agent is: 1%-5% by mass of chitosan (pH-responsive) / sodium carboxypropyl cellulose, 1%-5% titanium dioxide ultraviolet shielding agent, a small amount of silica, calcium chloride, magnesium sulfate and other moisture-proof substances, or a small amount of adhesive gelatin and polyvinyl butyral.

[0187] The method for preparing protein detection particles includes the following steps:

[0188] Using a masterbatch of fiber material as a carrier, the substrate colorimetric layer solution and the masterbatch are mixed at a weight ratio of 1:5-2:1. While stirring the particles, a peristaltic pump pressurizes the solution and sprays it onto the particles through a specially designed nozzle until the reagent solution is completely absorbed. Stirring continues until the mixture is homogeneous, the blank particles have uniformly absorbed the reagent solution, and no white particles remain. Stirring is then stopped, and the mixture is allowed to stand for 1-15 minutes. It is then dried once at 35-65℃ until the moisture content is below 10%. After spraying an embedding agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%. Finally, after spraying an encapsulating agent solution onto the surface, it is dried a second time at 35-65℃ until the moisture content is below 5%.

[0189] Collect genuine urine samples from dogs and felines, with dog and cat urine samples sourced from confirmed positive or negative samples obtained from veterinary hospitals. Collect urine samples from various mammals and add them dropwise onto a prepared granule (or a granule containing multiple samples), with 5 ml added to each sample. Observe and record the color reaction on the granule surface.

[0190] The clinical significance of each test item and the clinical value of combined interpretation are as follows:

[0191]

[0192]

[0193] Clinical significance of combined testing of various items:

[0194] Specific gravity, protein, occult blood, white blood cells;

[0195] Glomerulonephritis (nephritis syndrome): mainly characterized by elevated occult blood, with increased white blood cells, protein, and specific gravity.

[0196] Nephrotic syndrome: characterized by elevated protein levels (greater than 3.5g / day), with possible occult blood and elevated white blood cell count.

[0197] Occult blood, white blood cells, nitrite;

[0198] Infections of the urinary system and adjacent organs: Primarily characterized by leukocytosis; infections with Escherichia coli and other bacteria possessing nitrate reductase lead to elevated nitrite levels. Elevated red blood cell counts may also be observed. Red blood cell phase imaging suggests non-glomerular hematuria or mixed hematuria.

[0199] Glucose, ketone bodies, specific gravity;

[0200] Diabetes mellitus: Glycosuria occurs when blood glucose exceeds the renal threshold for glucose (8.88 mmol / L). Specific gravity increases.

[0201] Diabetic ketoacidosis: When diabetes is uncontrolled, elevated blood ketone bodies lead to elevated urine ketone bodies. Ketone bodies are helpful in the early diagnosis of diabetic ketoacidosis.

[0202] Bilirubin, urobilinogen;

[0203] Used for the diagnosis of jaundice and the differential diagnosis of different types of jaundice. The specific differentiation of different types of jaundice is shown in the table below.

[0204] A positive bilirubin result is seen in hepatocellular or obstructive jaundice and congenital hyperbilirubinemia. It is negative in hemolytic jaundice.

[0205] A positive result for urobilinogen is seen in hemolytic or hepatocellular jaundice, while it is negative in obstructive jaundice.

[0206] Laboratory differentiation between normal and different types of jaundice

[0207]

[0208] Secondly, a multi-index mixable urine test particle for pet urine is prepared using the multi-index mixable urine test particle preparation process described in the first aspect. It includes an inner reaction layer, a middle polyethylene glycol (PEG-6000 / 8000) antioxidant layer, and an outer hydroxypropyl methylcellulose moisture-proof film. The outer hydroxypropyl methylcellulose moisture-proof film also contains titanium dioxide ultraviolet shielding agent, and has a light transmittance of <5%.

[0209] The above description is merely a preferred embodiment of the present invention for multi-index mixable urine test granules for pet urine and its preparation process. Of course, it should not be construed as limiting the scope of the present invention. Those skilled in the art can understand that all or part of the above embodiments can be implemented, and equivalent changes made in accordance with the claims of the present invention are still within the scope of the invention.

Claims

1. A process for preparing multi-index mixable urine test particles from pet urine, characterized in that, Includes the following steps: Using the masterbatch as a carrier, the substrate color development layer solution and the masterbatch are mixed at a preset weight ratio, stirred evenly, and then allowed to stand. Then, a drying process is performed to obtain semi-finished product one. After spraying an embedding agent solution onto the surface of semi-finished product one, a secondary drying process is performed to obtain semi-finished product two. After spraying a coating agent solution onto the surface of the semi-finished product, it undergoes three drying processes to obtain urine test particles.

2. The preparation process for multi-index mixable urine test particles for pet urine as described in claim 1, characterized in that, In the process of "using masterbatch as a carrier, mixing the substrate color development layer solution with masterbatch at a preset weight ratio, stirring evenly and then letting it stand, followed by a drying process to obtain semi-finished product one", the masterbatch is a fiber material, bentonite material or silica material; the length of the fiber material, bentonite material and silica material fibers is 1-25mm; the fiber material is selected from one or more of paper fiber, bamboo fiber, coconut shell fiber, flax fiber, cotton fiber, sisal fiber, polyester fiber, nylon fiber and polyacrylonitrile fiber.

3. The preparation process for multi-index mixable urine test particles for pet urine as described in claim 1, characterized in that, In the process of "using masterbatch as a carrier, mixing the substrate chromogenic layer solution with the masterbatch at a preset weight ratio, stirring evenly and then letting it stand, and then performing a drying process to obtain semi-finished product one", the substrate chromogenic layer solution includes a reaction substrate, a chromogenic agent, a promoter, a free radical scavenger, a metal chelating agent and a buffer solution.

4. The preparation process for multi-index mixable urine test particles for pet urine as described in claim 1, characterized in that, In the phrase "after spraying an embedding agent solution onto the surface of semi-finished product one, a secondary drying process is performed to obtain semi-finished product two", the embedding agent solution is polyethylene glycol (PEG-6000 / 8000).

5. The preparation process for multi-index mixable urine test particles for pet urine as described in claim 1, characterized in that, In the phrase "after spraying a coating agent solution onto the surface of the semi-finished product and then performing three drying processes to obtain urine test particles", the coating agent is chitosan and hydroxypropyl methylcellulose.

6. The preparation process for multi-index mixable urine test particles for pet urine as described in claim 1, characterized in that, The temperature for the first drying process, the second drying process, and the third drying process is 35-65℃.

7. A multi-index mixable urine test granule for pet urine, prepared using the preparation process for multi-index mixable urine test granules for pet urine as described in any one of claims 1-6, characterized in that, It includes an inner reactive layer, a middle polyethylene glycol (PEG-6000 / 8000) antioxidant layer, and an outer hydroxypropyl methylcellulose moisture-proof film. The outer hydroxypropyl methylcellulose moisture-proof film also contains titanium dioxide ultraviolet shielding agent, and has a light transmittance of <5%.

Images