Full-automatic blood type analyzer based on centrifugal micro-fluidic chip

Abstract

The invention discloses a full-automatic blood type analyzer based on a centrifugal micro-fluidic chip, and belongs to the technical field of blood type analysis equipment. The analyzer comprises a bottom plate, wherein a sample reagent bin module, a chip processing module, a new chip storage bin module, a chip recycling bin module, a sample adding arm module and a chip pushing hand module are arranged on the bottom plate; the chip pushing hand module is used for contacting and pushing the micro-fluidic chip to translate from the new chip storage bin module to the chip processing module for processing, and pushing the micro-fluidic chip processed by the chip processing module to translate to the chip recycling bin module for recycling; the sample adding arm module is positioned at the tops of the sample reagent bin module and the chip processing module and is used for adding a sample or a reagent. On the basis of the centrifugal micro-fluidic chip, full-automatic sample and reagent adding, chip transferring, chip loading, chip centrifuging, result detection and judgment and chip recycling are achieved, manual intervention is not needed, non-stop sample loading and sample result outputting are supported, and the size is smaller at the same testing speed.

Description

Technical Field

[0001] This invention relates to the field of detection technology, specifically to a fully automated blood typing analyzer based on a centrifugal microfluidic chip. Background Technology

[0002] With the continuous development of clinical science and in-depth research on blood types, blood typing has become increasingly indispensable, providing a definitive basis for clinical treatment and diagnosis. It is commonly used for blood type identification before clinical transfusion therapy, such as in cases of trauma, surgery, severe anemia, bleeding disorders (hemophilia), and chemotherapy for tumors, providing a basis for blood compatibility and serving as a crucial guarantee for safe transfusions. Neonatal hemolytic disease is a major cause of neonatal death, leading to fetal developmental arrest, malformations, miscarriage, and neonatal hyperbilirubinemia. Blood typing can predict the likelihood and severity of neonatal hemolytic disease as early as possible, providing a basis for its prevention and diagnosis. Furthermore, blood typing can also be applied in organ transplantation, forensic identification, and other fields.

[0003] There are various methods and forms of blood typing, such as serological and genetic blood typing. Considering economy, efficiency, and accuracy, serological blood typing is the most accurate and commonly used methodology in clinical medicine. Traditional manual and semi-automatic blood typing methods are not only cumbersome, inefficient, and lack precision, but also susceptible to human error due to the operator's experience, failing to meet the ever-increasing testing needs of medical institutions. Fully automated blood typing analyzers can effectively solve these problems.

[0004] Currently, the main types of fully automated blood typing analyzers on the market are cassette (microcolumn gel method) and plate (microplate method). Regardless of the type of equipment, due to the flat loading method of its consumables and methodological limitations, it is necessary to increase the size of the equipment in order to achieve high-throughput measurement speed requirements. This is a pain point for most medical institutions' laboratory departments. In addition, the internal structure of the equipment is complex and the maintenance cost is high.

[0005] The information disclosed in this background section is intended only to enhance the understanding of the overall background of the invention and should not be construed as an admission or in any way implying that the information constitutes prior art known to those skilled in the art. Summary of the Invention

[0006] Purpose of the invention: The technical problem to be solved by the present invention is to address the shortcomings of the prior art by providing a fully automated blood typing analyzer based on a centrifugal microfluidic chip, which is small in size, desktop, and occupies little space.

[0007] To solve the above-mentioned technical problems, the present invention discloses a fully automated blood typing analyzer based on a centrifugal microfluidic chip, including a base plate, wherein the base plate is provided with a sample reagent compartment module, a chip processing module, a new chip storage compartment module, a chip recovery compartment module, a sample dispensing arm module and a chip pusher module;

[0008] The chip pusher module is used to contact and push the microfluidic chip from the new chip storage module to the chip processing module for processing, and to push the microfluidic chip processed by the chip processing module to the chip recycling module for recycling.

[0009] The sample loading arm module is located at the top of the sample reagent chamber module and the chip processing module, and is used to add the sample or reagent in the sample reagent chamber module to the microfluidic chip in the chip processing module.

[0010] Specifically, the chip processing module includes a chip warming chamber assembly, a chip positioning centrifugation assembly, a chip centrifugation assembly, a chip reaction assembly, and an imaging detection assembly arranged sequentially along the chip transport direction; the chip warming chamber assembly is located between the chip positioning centrifugation assembly and the new chip storage chamber module, and the imaging detection assembly is located between the chip reaction assembly and the chip recovery chamber module; the sample dispensing arm module is located between the sample reagent chamber module and the chip positioning centrifugation assembly;

[0011] The chip positioning centrifugation component, the chip centrifugation component, and the imaging detection component each include at least a rotary motion mechanism, a rotating body fixing seat, and a lifting chip tray. The rotary motion mechanism is disposed on the base plate, and the rotating body fixing seat is connected to the output shaft of the rotary motion mechanism. The lifting chip tray can be vertically and vertically disposed on the base plate between the chip loading position and the chip mounting position along the Z-axis. When the lifting chip tray is at the chip loading position, the microfluidic chip it carries is located on the moving path of the chip pusher module. At this time, the chip pusher module can push the microfluidic chip currently carried by the lifting chip tray to the next station or push the microfluidic chip from the previous station into the current lifting chip tray. The pushed-in microfluidic chip is supported on the lifting chip tray and can move with the lifting chip tray. When the lifting chip tray descends to the chip mounting position, the microfluidic chip separates from the lifting chip tray and is circumferentially limited and sleeved on the outside of the rotating body fixing seat so as to rotate with the rotating body fixing seat.

[0012] More specifically, the chip reheating chamber assembly, the chip positionable centrifugation assembly, the chip centrifugation assembly, and the chip reaction assembly are arranged sequentially along the Y-axis; the chip reaction assembly, the imaging detection assembly, and the chip recovery chamber module are arranged sequentially along the X-axis.

[0013] The chip pusher module includes a first X-axis chip pusher assembly, a second X-axis chip pusher assembly, and a Y-axis chip pusher assembly, which are respectively directly or indirectly mounted on the base plate.

[0014] The first X-axis chip pusher assembly is provided with a first X-axis chip pusher, which performs linear reciprocating motion along the X-axis direction to push a new microfluidic chip from the new chip storage module to the chip reheating chamber assembly.

[0015] The Y-axis chip pusher assembly is equipped with a Y-axis chip pusher, which makes a linear reciprocating motion along the Y-axis direction to push out the microfluidic chip in the chip warming chamber assembly and pass it sequentially through the chip positionable centrifugal assembly, the chip centrifugal assembly, and the chip reaction assembly;

[0016] The second X-axis chip pusher assembly is provided with a second X-axis chip pusher, which performs linear reciprocating motion along the X-axis direction to push out the microfluidic chip in the chip reaction assembly and pass it sequentially through the imaging detection assembly and the chip recovery chamber module.

[0017] Specifically, the chip reheat chamber assembly includes:

[0018] The first multi-layer chip storage mechanism has multiple chip accommodating parts arranged along the Z-axis direction; each chip accommodating part is used to accommodate a microfluidic chip; the chip accommodating part has openings on both sides along the Y-axis direction corresponding to the Y-axis chip pusher of the Y-axis chip pusher assembly and the chip positioning centrifugal assembly, and has an opening on one side along the X-axis direction corresponding to the first X-axis chip pusher of the first X-axis chip pusher assembly.

[0019] And a first Z-axis linear drive mechanism, which is directly or indirectly mounted on the base plate and connected to the first multi-layer chip storage mechanism, for driving the first multi-layer chip storage mechanism to move linearly back and forth along the Z-axis direction;

[0020] When the chip receiving portion of the chip to be received moves onto the moving path of the first X-axis chip pusher assembly, the new microfluidic chip is pushed from the new chip storage module into the chip receiving portion of the chip to be received by the first X-axis chip pusher assembly; when the chip receiving portion of the chip to be transferred moves onto the moving path of the Y-axis chip pusher assembly, the warmed microfluidic chip is pushed into the chip positioning centrifugal assembly by the Y-axis chip pusher assembly.

[0021] Furthermore, the chip-positionable centrifugal assembly also includes a QR code scanning mechanism, and the rotational motion mechanism in the chip-positionable centrifugal assembly is a first rotational motion mechanism; the QR code scanning mechanism and the first rotational motion mechanism work together to complete the scanning of the QR code on the surface of the microfluidic chip.

[0022] Specifically, the chip reaction assembly includes a second multi-layer chip storage mechanism and a third Z-axis linear drive mechanism. The second multi-layer chip storage mechanism is used to receive and hold the microfluidic chip from the chip centrifugation assembly. The third Z-axis linear drive mechanism is configured to drive the second multi-layer chip storage mechanism to move linearly back and forth along the Z-axis direction, and cooperate with the Y-axis chip pusher assembly to transfer the held microfluidic chip to the imaging detection assembly.

[0023] Furthermore, the imaging detection component also includes an imaging mechanism, and the rotational motion mechanism in the imaging detection component is a rotational drive mechanism; the rotational drive mechanism can drive the microfluidic chip loaded on it to rotate at any position, and cooperate with the imaging mechanism to complete the imaging detection of each reaction hole in the microfluidic chip; after the detection is completed, the microfluidic chip is transferred to the chip recycling bin module under the drive of the second X-axis chip pusher of the second X-axis chip pusher component.

[0024] Specifically, the new chip storage module includes one or more storage units arranged side by side along the X-axis. Each storage unit includes a cabinet, a lifting chip compartment, and a sixth Z-axis linear motion mechanism corresponding to each lifting chip compartment. The lifting chip compartment has a accommodating space for storing multiple vertically stacked microfluidic chips. When the lifting chip compartment is in a descending position, the lifting chip compartment and the cabinet enclose the accommodating space, and the tops of all the lifting chip compartments and the cabinet together form a chip delivery channel. Each of the sixth Z-axis linear motion mechanisms is installed on the corresponding cabinet, and each lifting chip compartment is connected to the corresponding sixth Z-axis linear motion mechanism. Each sixth Z-axis linear motion mechanism is configured to drive the corresponding lifting chip compartment to perform linear lifting and lowering motion along the Z-axis.

[0025] The first X-axis chip pusher assembly is disposed on one side of the chip transport channel; the sixth Z-axis linear motion mechanism works together with the first X-axis chip pusher assembly to transfer the microfluidic chip in the lifting chip compartment along the chip transport channel to the chip processing module.

[0026] Specifically, the chip recycling bin module includes:

[0027] The outer frame of the recycling bin has a pull-out space inside;

[0028] A chip collection device for collecting used chips, wherein the chip collection device is detachably disposed within the outer frame of the recycling bin in an extended position for extending the extended space and a retracted position for retracting the extended space; a chip drop outlet is provided at the top of the extended space corresponding to the position of the chip collection device in the retracted position.

[0029] A pair of guide rails are disposed above the chip drop port. The pair of guide rails have a first state in which they are close to each other to carry the used microfluidic chip from the chip processing module, and a second state in which they are far apart to allow the used microfluidic chip they carry to fall through the chip drop port into the chip collection device.

[0030] And a guide rail opening drive mechanism for driving the pair of guide rails to move closer to each other and further apart, the guide rail opening drive mechanism being mounted on the base plate through the outer frame of the recycling bin; the pair of guide rails being connected to the guide rail opening drive mechanism.

[0031] Specifically, the sample reagent compartment module includes:

[0032] A reagent rotary mixing mechanism for loading one or more reagents for mixing;

[0033] A reagent loading mechanism for loading one or more reagents that do not require mixing;

[0034] A sample rack mechanism for mounting multiple sampling tubes;

[0035] The first Y-axis linear drive mechanism is directly or indirectly mounted on the base plate; the reagent rotation mixing mechanism and the reagent loading mechanism are respectively connected to the first Y-axis linear drive mechanism, and the first Y-axis linear drive mechanism is configured to drive the reagent rotation mixing mechanism and the reagent loading mechanism to reciprocate synchronously along the Y-axis direction;

[0036] And a sample rack pusher mechanism, which is installed on the base plate and connected to the sample rack mechanism, wherein the sample rack pusher mechanism is configured to drive the sample rack mechanism to reciprocate along the Y-axis direction;

[0037] The sample dispensing arm module includes:

[0038] First injection needle assembly;

[0039] Second injection needle assembly;

[0040] The second mounting bracket is fixedly mounted on the base plate;

[0041] The first X-axis linear motion mechanism and the second X-axis linear motion mechanism are respectively installed on the second mounting frame at intervals;

[0042] The fourth Z-axis linear motion mechanism is mounted on the first X-axis linear motion mechanism and connected to the first dispensing needle assembly;

[0043] The fifth Z-axis linear motion mechanism is mounted on the second X-axis linear motion mechanism and connected to the second filling needle assembly;

[0044] The device includes a first injection needle cleaning position and a second injection needle cleaning position, wherein the first injection needle cleaning position is located on the movement path of the first injection needle assembly, and the second injection needle cleaning position is located on the movement path of the second injection needle assembly.

[0045] Beneficial effects:

[0046] 1. The fully automated blood typing analyzer based on centrifugal microfluidic chip provided in this application achieves dual-needle collaborative sample addition through the cooperation of the sample dispensing arm module and the sample reagent compartment module, reducing waiting time; through the automatic loading of the new microfluidic chip, the chip is processed and transported in sequence in the chip processing module, and the chip is automatically collected after use, realizing high-throughput fully automated analysis without manual intervention, supporting sample loading without stopping the machine, sample in and results out, and has a smaller instrument size at the same testing speed.

[0047] 2. The fully automated blood typing analyzer based on centrifugal microfluidic chip provided in this application integrates the sample reagent chamber module, chip processing module, sample dispensing arm module, new chip storage chamber module, chip recovery chamber module and chip pusher module on the base plate to form a desktop device with small size.

[0048] 3. This application adopts dual-needle collaborative sampling, which can reduce the use of TIP consumables, lower operating costs, simplify equipment structure, and improve equipment stability compared to traditional workstation-type fully automated blood typing analyzers.

[0049] 4. In the chip processing module of this application, components that must rotate the chip during processing, such as the chip positioning centrifugation component, the chip centrifugation component, and the imaging detection component, are all designed so that the centrifugal microfluidic chip can be automatically unloaded when moved upward and automatically loaded when moved downward relative to the rotating motion mechanism under the action of the lifting chip tray. This allows the analyzer of this application to use a pusher component as the chip transfer mechanism. Compared with the suction cup transfer mechanism, using a pusher component as the chip transfer mechanism has at least the following advantages: 1) Mechanical contact, reliable, and not dependent on the cleanliness and flatness of the chip surface; 2) Only contacting the chip edge, not the top surface of the chip, reducing the risk of contamination; 3) Contacting and pushing the chip, compared with the concentrated suction of the suction cup, suitable for fragile and thin chips; 4) Motor driven, no need for air circuits and sensors, low cost, small space occupation, suitable for desktop equipment and a cleaner internal structure; 5) Direct pushing, which is more efficient than pneumatic; 6) Contacting the side edge of the chip, the chip does not need to reserve an adsorption area, which is helpful for the design and manufacturing of the chip itself. Attached Figure Description

[0050] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments, and the advantages of the present invention in the above and / or other aspects will become clearer.

[0051] Figure 1 A three-dimensional structural schematic diagram of a fully automated blood typing analyzer based on a centrifugal microfluidic chip provided for an embodiment of the present invention;

[0052] Figure 2 for Figure 1 A three-dimensional structural diagram of the assembly structure of the chip processing module, the second X-axis chip pusher assembly, and the Y-axis chip pusher assembly in the fully automated blood typing analyzer shown.

[0053] Figure 3 for Figure 1 A three-dimensional structural diagram of the sample reagent compartment module in the fully automated blood typing analyzer shown;

[0054] Figure 4 for Figure 1 A three-dimensional structural diagram of the sample dispensing arm module in the fully automated blood typing analyzer shown;

[0055] Figure 5 for Figure 1 The diagram shows the three-dimensional structure of the new chip storage module in the fully automated blood typing analyzer. Figure 1 ;

[0056] Figure 6 for Figure 1 The diagram shows the three-dimensional structure of the new chip storage module in the fully automated blood typing analyzer. Figure 2 ;

[0057] Figure 7 for Figure 1 The diagram shows the three-dimensional structure of the new chip storage module in the fully automated blood typing analyzer. Figure 3 ;

[0058] Figure 8 for Figure 1 A three-dimensional structural diagram of the chip recovery chamber module in the fully automated blood typing analyzer shown;

[0059] Figure 9 for Figure 8 A three-dimensional structural diagram of the guide rail opening drive mechanism in the chip recycling bin module shown.

[0060] Figure 10 for Figure 1 The diagram shows the assembly structure of the base plate, power supply unit, water-cooled circulating pump, water-cooled heat sink, frame, and plunger pump in the fully automated blood typing analyzer.

[0061] The accompanying figure labels are explained as follows:

[0062] 10. Sample reagent compartment module; 101. Reagent rotary mixing mechanism; 1011. Rotary mixing mechanism; 102. Reagent loading mechanism; 1012. Mixing drive mechanism; 103. Sample rack mechanism; 104. First Y-axis linear drive mechanism; 105. Sample rack pusher mechanism; 106. First mounting frame; 20. Chip processing module; 201. Chip warming chamber assembly; 202. Chip positioning centrifuge assembly; 203. Chip centrifuge assembly; 204. Chip reaction assembly; 205. Imaging detection assembly; 206. Fixed support assembly; 209. Imaging mechanism; 210. First lifting chip tray; 211. Second lifting chip tray; 212. Third lifting chip tray; 213. QR code scanner 214. First rotary motion mechanism; 215. High-speed centrifugal drive mechanism; 216. Rotary drive mechanism; 217. First Z-axis linear drive mechanism; 218. Third Z-axis linear drive mechanism; 219. Y-axis chip pusher drive mechanism; 220. First multi-layer chip storage mechanism; 221. Second multi-layer chip storage mechanism; 30. Sample dispensing arm module; 301. First dispensing needle assembly; 302. Second dispensing needle assembly; 303. First X-axis linear motion mechanism; 304. Second X-axis linear motion mechanism; 305. Needle washing mechanism; 3051. First dispensing needle cleaning position; 3052. Second dispensing needle cleaning position; 306. Second mounting bracket; 308. Fifth Z-axis linear motion mechanism; 309. 40. Four Z-axis linear motion mechanism; 40. New chip storage compartment module; 401. Lifting chip compartment; 4011. First lifting chip compartment; 4012. Second lifting chip compartment; 4013. Third lifting chip compartment; 4021. First sixth Z-axis linear motion mechanism; 4022. Second sixth Z-axis linear motion mechanism; 4023. Third sixth Z-axis linear motion mechanism; 406. Cabinet; 4101. First refrigeration component; 4102. Second refrigeration component; 4103. Third refrigeration component; 411. Semiconductor cooling chip; 412. Water tank assembly; 50. Chip recycling compartment module; 501. Guide rail; 502. Guide rail opening drive mechanism; 5021. Drive motor; 5022. Transmission synchronous belt; 5023. 5024. Screw mechanism; 5025. Linear guide rail; 5026. First guide rail lever; 5027. Second guide rail lever; 508. First chip limit lever; 509. Second chip limit lever; 510. Third chip limit lever; 501. Support base; 502. Outer frame of the recovery bin; 502. Pull-out space; 510. Chip drop port; 601. Power module; 602. Base plate; 603. Frame; 604. Piston pump; 605. Water-cooled circulating pump; 606. Water-cooled heat sink; 607. External switch; 608. External interface; 70. Chip pusher module; 701. Y-axis chip pusher assembly; 702. Second X-axis chip pusher assembly; 703. First X-axis chip pusher assembly; 80. Microfluidic chip. Detailed Implementation

[0063] The specific structure of the embodiments of the present invention is as follows: Figures 1 to 8 As shown. In this embodiment, the X-axis, Y-axis and Z-axis are perpendicular to each other, the Z-axis is set in the vertical direction, and the X-axis and Y-axis are located in the horizontal plane.

[0064] In this invention, the microfluidic chip 80 or chip refers to a centrifugal microfluidic chip, which can be a segmented centrifugal microfluidic chip including a tray and a disc. The pusher-transfer chip in this invention refers to a chip that is contacted and pushed by a pusher.

[0065] like Figure 1 As shown, this embodiment of the fully automated blood typing analyzer based on centrifugal microfluidic chips includes a base plate 602. The base plate 602 is equipped with a sample reagent compartment module 10, a chip processing module 20, a sample dispensing arm module 30, a new chip storage compartment module 40, a chip recovery compartment module 50, and a chip pusher module 70. The new chip storage compartment module 40 stores new microfluidic chips 80; the chip recovery compartment module 50 stores used microfluidic chips 80; the chip pusher module 70 contacts and pushes the microfluidic chip 80 from the new chip storage compartment module 40 to the chip processing module 20 for processing, and pushes the microfluidic chip 80 processed by the chip processing module 20 to the chip recovery compartment module 50 for recovery; the sample dispensing arm module 30 is located on top of the sample reagent compartment module 10 and the chip processing module 20, and is used to add samples or reagents from the sample reagent compartment module 10 to the corresponding microfluidic chips 80 in the chip processing module 20 to meet the requirements of blood typing analysis.

[0066] like Figure 2 As shown, the chip processing module 20 includes a fixed support assembly 206 and a chip warming chamber assembly 201, a chip positioning centrifugation assembly 202, a chip centrifugation assembly 203, a chip reaction assembly 204, and an imaging detection assembly 205, which are respectively fixedly integrated onto the base plate 602 by the fixed support assembly 206 and arranged sequentially along the chip transport direction. A sample loading arm module 30 is located between the sample reagent chamber module 10 and the chip positioning centrifugation assembly 202, and is used to add samples or reagents from the sample reagent chamber module 10 into the microfluidic chip 80 within the chip positioning centrifugation assembly 202 to meet the requirements of blood typing analysis.

[0067] The chip positioning centrifugation component 202, the chip centrifugation component 203, and the imaging detection component 205 each include at least a rotary motion mechanism, a rotary body fixing seat, and a lifting chip tray. The rotary motion mechanism is disposed on the base plate 602, the rotary body fixing seat is connected to the output shaft of the rotary motion mechanism, and the lifting chip tray can be vertically and vertically disposed on the base plate 602 between the chip feeding position and the chip loading position along the Z-axis direction. When the lifting chip tray is in the chip loading position, the microfluidic chip 80 it carries is located on the moving path of the chip pusher module. At this time, the chip pusher module 70 can push the microfluidic chip 80 carried by the current lifting chip tray to the next station or push the microfluidic chip 80 from the previous station into the current lifting chip tray. The pushed-in microfluidic chip 80 is supported on the lifting chip tray and can move with the lifting chip tray. When the lifting chip tray descends to the chip loading position, the microfluidic chip 80 separates from the lifting chip tray and is circumferentially limited and sleeved on the outside of the rotating body fixing seat so as to rotate with the rotating body fixing seat. The connection structure between the rotating body fixing seat and the microfluidic chip 80 in this embodiment adopts the connecting device for a rotating body for medical testing disclosed in Chinese Utility Model Patent Publication No. CN222510960U. The rotating body fixing seat adopts the rotating body fixing seat in this Chinese Utility Model Patent, and the microfluidic chip 80 adopts the structure of a rotating body. When the microfluidic chip 80 is a chipped centrifugal microfluidic chip, the centering guide groove and the limiting groove of the rotating body are both formed on the disk support.

[0068] More specifically, see Figure 2 The chip reheating chamber assembly 201, the chip positioning centrifugation assembly 202, the chip centrifugation assembly 203, and the chip reaction assembly 204 are arranged sequentially along the Y-axis. The chip reaction assembly 204, the imaging detection assembly 205, and the chip recovery chamber module 50 are arranged sequentially along the X-axis. See also Figure 1The chip pusher module 70 includes a first X-axis chip pusher assembly 703, a second X-axis chip pusher assembly 702, and a Y-axis chip pusher assembly 701, which are respectively directly or indirectly mounted on the base plate 602. The first X-axis chip pusher assembly 703 is provided with a first X-axis chip pusher, which performs linear reciprocating motion along the X-axis direction to push the new microfluidic chip 80 from the new chip storage chamber module 40 to the chip reheat chamber assembly 201. The Y-axis chip pusher assembly 701 is provided with a Y-axis chip pusher, which performs linear reciprocating motion along the Y-axis direction to push the microfluidic chip 80 out of the chip reheat chamber assembly 201 and sequentially pass through the chip positionable centrifuge assembly 202, the chip centrifuge assembly 203, and the chip reaction assembly 204. The second X-axis chip pusher assembly 702 is equipped with a second X-axis chip pusher. The second X-axis chip pusher makes a linear reciprocating motion along the X-axis direction to push out the microfluidic chip 80 in the chip reaction assembly 204 and pass through the imaging detection assembly 205 and the chip recycling bin module 50 in sequence.

[0069] It should be understood that each pusher component in this embodiment includes a pusher drive mechanism for driving the corresponding chip pusher to reciprocate linear motion. The pusher drive mechanism is directly or indirectly mounted to the base plate 602. The pusher drive mechanism can be an existing pusher drive mechanism. The specific structure of the pusher drive mechanism is not the main improvement point of this application, so it will not be described in detail here. Figure 2 A Y-axis chip pusher drive mechanism 219 for driving a Y-axis chip pusher to reciprocate linear motion along the Y-axis direction has been shown.

[0070] In one embodiment, such as Figure 3 As shown, the sample reagent compartment module 10 includes a reagent rotation mixing mechanism 101, a reagent loading mechanism 102, a sample rack mechanism 103, a first Y-axis linear drive mechanism 104, a sample rack pusher mechanism 105, and a first mounting frame 106. The sample rack mechanism 103 is used to load multiple sampling tubes, and the reagent loading mechanism 102 is used to load one or more reagents that do not require mixing. The reagent rotation mixing mechanism 101 is used to load one or more reagents that need to be mixed. The first Y-axis linear drive mechanism 104 and the sample rack pusher mechanism 105 are both mounted on the base plate 602 via the first mounting frame 106. The reagent rotation mixing mechanism 101 and the reagent loading mechanism 102 are respectively connected to the first Y-axis linear drive mechanism 104, which is configured to drive the reagent rotation mixing mechanism 101 and the reagent loading mechanism 102 to reciprocate synchronously along the Y-axis direction. The sample rack mechanism 103 is connected to the sample rack pusher mechanism 105, which is configured to drive the sample rack mechanism 103 to reciprocate along the Y-axis.

[0071] like Figure 4As shown, the sample dispensing arm module 30 includes a second mounting bracket 306, a first dispensing needle assembly 301, a second dispensing needle assembly 302, a first X-axis linear motion mechanism 303, a fourth Z-axis linear motion mechanism 309, a second X-axis linear motion mechanism 304, and a fifth Z-axis linear motion mechanism 308. The second mounting bracket 306 is fixedly mounted on the base plate 602. The first X-axis linear motion mechanism 303 and the second X-axis linear motion mechanism 304 are respectively and spaced apart from each other on the second mounting bracket 306. The fourth Z-axis linear motion mechanism 309 is mounted on the first X-axis linear motion mechanism 303. The first dispensing needle assembly 301 is mounted on the fourth Z-axis linear motion mechanism 309. The fifth Z-axis linear motion mechanism 308 is mounted on the second X-axis linear motion mechanism 304. The second dispensing needle assembly 302 is mounted on the fifth Z-axis linear motion mechanism 308.

[0072] In this embodiment, the first injection needle assembly 301 is driven by the first X-axis linear motion mechanism 303 and the fourth Z-axis linear motion mechanism 309 to complete its linear movement and positioning in the X-axis and Z-axis directions, and the second injection needle assembly 302 is driven by the second X-axis linear motion mechanism 304 and the fifth Z-axis linear motion mechanism 308 to complete its linear movement and positioning in the X-axis and Z-axis directions.

[0073] like Figures 2 to 4 As shown, the sample dispensing arm module 30 works in conjunction with the sample reagent compartment module 10 to aspirate any reagent or sample from the reagent rotation mixing mechanism 101, the reagent loading mechanism 102, and the sample rack mechanism 103 via the first dispensing needle assembly 301 and the second dispensing needle assembly 302, and then transfer it to the microfluidic chip 80 loaded within the chip-positionable centrifugation assembly 202. This embodiment achieves dual-needle coordinated sample dispensing through the combined operation of the sample reagent compartment module 10 and the sample dispensing arm module 30, reducing waiting time.

[0074] Specifically, such as Figure 3 As shown, the reagent rotary mixing mechanism 101 includes a rotary mixing mechanism 1011 and a mixing drive mechanism 1012 connected to the rotary mixing mechanism 1011. The rotary mixing mechanism 1011 has multiple mixing positions, and the mixing drive mechanism 1012 is configured to drive the multiple mixing positions of the rotary mixing mechanism 1011 to mix simultaneously. The reagent rotary mixing mechanism 101 can adopt a reagent rotary mixing mechanism in the prior art, and its specific structure is not the main improvement point of this application, so it will not be described in detail here.

[0075] Furthermore, such as Figure 4As shown, the sample dispensing arm module 30 also includes a needle washing mechanism 305, which includes a first dispensing needle cleaning position 3051 and a second dispensing needle cleaning position 3052. The first dispensing needle cleaning position 3051 is located on the movement path of the first dispensing needle assembly 301, and the second dispensing needle cleaning position 3052 is located on the movement path of the second dispensing needle assembly 302. The needle washing mechanism 305 can be installed on the first mounting bracket 106.

[0076] In this embodiment, the inner and outer walls of the injection needles of the first injection needle assembly 301 and the second injection needle assembly 302 are cleaned by the needle washing mechanism 305 to avoid cross-contamination.

[0077] Specifically, such as Figure 2 As shown, the chip reheating chamber assembly 201 includes: a first multi-layer chip storage mechanism 220, which has multiple chip accommodating portions arranged along the Z-axis; each chip accommodating portion is used to accommodate a microfluidic chip 80; the chip accommodating portions have openings on both sides along the Y-axis corresponding to the Y-axis chip pusher assembly 701 and the chip positioning centrifugal assembly 202, and an opening on one side along the X-axis corresponding to the first X-axis chip pusher assembly 703; and a first Z-axis linear drive mechanism 217, which is directly or indirectly mounted on the base plate 602 and connected to the first multi-layer chip storage mechanism 220, for driving the first multi-layer chip storage mechanism 80. The layer chip storage mechanism 220 moves linearly back and forth along the Z-axis. When the chip receiving part of the chip to be received moves to the moving path of the first X-axis chip pusher assembly 703, the new microfluidic chip 80 is pushed from the new chip storage module 40 into the chip receiving part of the chip to be received by the first X-axis chip pusher assembly 703. When the chip receiving part of the chip to be transferred moves to the moving path of the Y-axis chip pusher assembly 701, the warmed microfluidic chip 80 is pushed into the chip positioning centrifugal assembly 202 by the Y-axis chip pusher assembly 701.

[0078] In this application, "warming up" refers to leaving the chip at room temperature for a period of time after it has been transferred out of the new chip storage module 40, so as to ensure that the liquid reagents inside the chip return to room temperature from a low temperature state, so as not to affect the experimental results.

[0079] Specifically, such as Figure 2As shown, the lifting chip tray in the chip-positionable centrifugal assembly 202 is the first lifting chip tray 210, and the rotary motion mechanism in the chip-positionable centrifugal assembly 202 is the first rotary motion mechanism 214. The first lifting chip tray 210, located at the chip loading position, can receive the microfluidic chip 80 from the chip reheat chamber assembly 201. The microfluidic chip 80 can be loaded onto or unloaded from the first rotary motion mechanism 214 via the first lifting chip tray 210. After loading, the microfluidic chip 80 completes a high-speed centrifugal motion under the drive of the first rotary motion mechanism 214, and can achieve high-precision positioning motion as required.

[0080] Furthermore, the first liftable chip tray 210 can also be configured to be controllably heated to incubate the microfluidic chip 80 it carries. Specifically, controllably heating of the first liftable chip tray 210 is achieved by arranging heating elements, such as thin-film heaters, on the bearing surface of the first liftable chip tray 210.

[0081] Furthermore, the chip-positionable centrifugal assembly 202 also includes a QR code scanning mechanism 213, which works in conjunction with the first rotary motion mechanism 214 to complete the QR code scanning on the surface of the microfluidic chip 80 and identify the item information of the microfluidic chip 80.

[0082] Specifically, such as Figure 2 As shown, the lifting chip tray of the chip centrifugation assembly 203 is a second lifting chip tray 211, and the rotation mechanism of the chip centrifugation assembly 203 is a high-speed centrifugation drive mechanism 215. The microfluidic chip 80 can be loaded onto or unloaded from the high-speed centrifugation drive mechanism 215 via the second lifting chip tray 211. The high-speed centrifugation of the microfluidic chip 80 is completed by the high-speed centrifugation drive mechanism 215, and the centrifuged microfluidic chip 80 can be transferred to the chip reaction assembly 204 in conjunction with the Y-axis chip pusher assembly 701.

[0083] like Figure 2 As shown, the chip reaction assembly 204 includes a second multi-layer chip storage mechanism 221 and a third Z-axis linear drive mechanism 218. The second multi-layer chip storage mechanism 221 is used to receive and hold the microfluidic chip 80 from the second lifting chip tray 211. The third Z-axis linear drive mechanism 218 is configured to drive the second multi-layer chip storage mechanism 221 to move linearly back and forth along the Z-axis direction, and cooperate with the Y-axis chip pusher assembly 701 to transfer the microfluidic chip 80 after the reaction to the imaging detection assembly 205.

[0084] Specifically, the second multilayer chip storage mechanism 221 can adopt the same structure as the first multilayer chip storage mechanism 220. The difference is that the chip accommodating portion of the second multilayer chip storage mechanism 221 has openings on both sides along the X-axis direction corresponding to the second X-axis chip pusher assembly 702 and the imaging detection assembly 205, and an opening on one side along the Y-axis direction corresponding to the Y-axis chip pusher assembly 701. The third Z-axis linear drive mechanism 218 can drive the second multilayer chip storage mechanism 221 to move linearly back and forth along the Z-axis direction, so that the chip accommodating portion of the chip to be received moves to the moving path of the Y-axis chip pusher assembly 701, so that the Y-axis chip pusher assembly 701 can push the microfluidic chip 80 from the chip centrifugation assembly 203 into the chip accommodating portion of the chip to be received, or move the chip accommodating portion of the chip to be transferred to the moving path of the second X-axis chip pusher assembly 702, so that the second X-axis chip pusher assembly 702 can push the microfluidic chip 80 after static reaction into the imaging detection assembly 205.

[0085] Specifically, such as Figure 2 As shown, the lifting chip tray of the imaging detection component 205 is a third lifting chip tray 212, and the rotation mechanism in the imaging detection component 205 is a rotation drive mechanism 216. The imaging detection component 205 also includes an imaging mechanism 209. The third lifting chip tray 212 is used to receive the microfluidic chip 80 from the chip reaction component 204 and load it onto or unload it from the rotation drive mechanism 216. The rotation drive mechanism 216 can drive the loaded microfluidic chip 80 to rotate to any position, and cooperate with the imaging mechanism 209 to complete the imaging detection of each reaction hole in the microfluidic chip 80. After the detection is completed, the microfluidic chip 80 is transferred to the chip recycling bin module 50 under the drive of the second X-axis chip pusher of the second X-axis chip pusher component 702.

[0086] Specifically, such as Figures 5 to 7As shown, the new chip storage module 40 includes one or more storage units arranged side by side along the X-axis. Each storage unit includes a cabinet 406, a lifting chip compartment 401, and a sixth Z-axis linear motion mechanism corresponding to each lifting chip compartment 401. The lifting chip compartment 401 has a accommodating space for storing multiple vertically stacked microfluidic chips 80. When the lifting chip compartment 401 is in the lowered position, the lifting chip compartment 401 and the cabinet 406 enclose the accommodating space, and the tops of all the lifting chip compartments 401 and the cabinet 406 together form a chip delivery channel. Each sixth Z-axis linear motion mechanism is installed on the corresponding cabinet 406, and each lifting chip compartment 401 is connected to the corresponding sixth Z-axis linear motion mechanism. Each sixth Z-axis linear motion mechanism is configured to drive the corresponding lifting chip compartment 401 to perform linear lifting and lowering motion along the Z-axis. The first X-axis chip pusher assembly 703 is located on one side of the chip transport channel; the sixth Z-axis linear motion mechanism works together with the first X-axis chip pusher assembly 703 to transfer the microfluidic chip 80 in the lifting chip compartment 401 along the chip transport channel into the chip processing module 20.

[0087] Specifically, when one of the lifting chip chambers 401 rises to the top and the microfluidic chip 80 is on the pusher movement path of the first X-axis chip pusher assembly 703, the other lifting chip chambers 401 are in the descending position. At this time, the pusher of the first X-axis chip pusher assembly 703 contacts and pushes the microfluidic chip 80 along the chip transport channel to a chip receiving part in the chip processing module 20.

[0088] See Figure 7 and Figure 10 The analyzer also includes a cryogenic refrigeration system, which includes refrigeration components corresponding to each storage compartment unit, a water-cooled circulating pump 605, and a water-cooled heat sink 606. Each refrigeration component includes a thermoelectric cooler 411 and a water tank assembly 412. See [link to relevant documentation]. Figure 7 In each cooling component, the cold end of the thermoelectric cooler 411 faces upward and is attached to the bottom of the cabinet 406. The water tank assembly 412 is located at the hot end of the thermoelectric cooler 411. When cooling the new chip storage module 40, the thermoelectric cooler 411 generates heat at its hot end. The water tank assembly 412, the water-cooled circulation pump 605, and the water-cooled radiator 606 are connected by pipes to form a refrigerant circulation loop. The water-cooled circulation pump 605 drives the refrigerant in the pipes to flow sequentially through each water tank assembly 412, and finally reaches the water-cooled radiator 606, where it exchanges the heat absorbed by the refrigerant to the outside.

[0089] Specifically, see Figure 10The low-temperature refrigeration system also includes a frame 603, which is mounted on a base plate 602. A water-cooled circulating pump 605 and a water-cooled heat sink 606 are respectively mounted on the frame 603.

[0090] Specifically, such as Figure 10 As shown, this includes a power supply enclosure assembly mounted on a base plate 602. More specifically, as... Figure 10 As shown, the power supply box assembly includes a power supply box body 601, an external switch 607, and an external interface 608. The external switch 607 and external interface 608 are fixed to the power supply box body 601. The power supply box body 601 integrates the power modules, filters, and other components required for the entire device, facilitating wiring and maintenance. The external switch 607 serves as both the power supply interface and the power control interface. The external interface 608 is the device communication interface used for device debugging.

[0091] Furthermore, such as Figure 10 As shown, it also includes a plunger pump 604, which is configured to perform suction and ejection of liquid within the filling needles of the first filling needle assembly 301 and the second filling needle assembly 302. The plunger pump 604 is mounted on the base plate 602.

[0092] Specifically, such as Figure 1 and Figure 8 As shown, the chip recycling module 50 includes: a recycling bin outer frame 508, with a pull-out space 509 inside the recycling bin outer frame 508; a chip collecting device for collecting used chips, the chip collecting device being pull-outly disposed within the recycling bin outer frame 508 in both the pull-out and retracted positions of the pull-out space 509; a chip drop outlet 510 located at the top of the pull-out space 509 corresponding to the position of the chip collecting device in the retracted position; and a pair of guide rails 501 disposed above the chip drop outlet 510. A pair of guide rails 501 have a first state in which they are close to each other to carry the used microfluidic chip 80 from the chip processing module 20, and a second state in which they are spread apart to allow the used microfluidic chip 80 to fall into the chip collection device through the chip drop port 510; and a guide rail spreading drive mechanism 502 for driving the pair of guide rails 501 to move closer and further apart, the guide rail spreading drive mechanism 502 being mounted on the base plate 602 through the outer frame 508 of the recycling bin; the pair of guide rails 501 are connected to the guide rail spreading drive mechanism 502.

[0093] Specifically, see Figure 9The guide rail opening drive mechanism 502 includes a drive motor 5021, a transmission synchronous belt 5022, a threaded screw mechanism 5023, a linear guide rail 5024, a first guide rail lever 5025, and a second guide rail lever 5026. The drive motor 5021, linear guide rail 5024, and threaded screw mechanism 5023 are respectively mounted on the outer frame 508 of the recovery bin. The first guide rail lever 5025 and the second guide rail lever 5026 are threadedly connected to the threaded screw of the threaded screw mechanism 5023. One of the pair of guide rails 501 is connected to the first guide rail lever 5025, and the other is connected to the second guide rail lever 5026. The drive motor 5021 and the threaded screw mechanism 5023 are connected via the transmission synchronous belt 5022 to drive the threaded screw of the threaded screw mechanism 5023 to rotate. When the drive motor 5021 drives the lead screw of the lead screw mechanism 5023 to rotate, the lead screw mechanism 5023 drives the first guide rail lever 5025 and the second guide rail lever 5026 to move in opposite directions or in opposite directions along the linear guide rail 5024. By changing the rotation direction of the drive motor 5021, the direction of linear movement of the first guide rail lever 5025 and the second guide rail lever 5026 is controlled, thereby controlling the movement of the pair of guide rails 501 towards and away from each other.

[0094] More specifically, see Figure 8 The chip collection device includes: a support base 507 for supporting the microfluidic chip 80 after use, the support base 507 being provided with one or more collection positions for stacking the microfluidic chip 80 after use; the one or more collection positions being spaced apart along the extension direction of the guide rail 501; and a chip limiting part for stopping the circumferential displacement of the microfluidic chip 80, each collection position being provided with a corresponding chip limiting part.

[0095] In this embodiment, the first X-axis chip pusher assembly 703 drives the used microfluidic chip 80 to move on a pair of guide rails 501 in the first state. When the used microfluidic chip 80 moves directly above one of the collection positions, the guide rail opening drive mechanism 502 drives the pair of guide rails 501 to open to the second state, and the used microfluidic chip 80 falls and stacks on top of the topmost microfluidic chip 80 already collected at the collection position. The chip limiting part limits the stacked microfluidic chips 80 at the collection position.

[0096] More specifically, see Figure 8 The chip limiting part is a chip limiting rod, which is configured to allow the microfluidic chip 80 to be fitted onto it. The distance between two adjacent chip limiting rods is greater than the outer diameter of the microfluidic chip 80.

[0097] In one particular embodiment, see Figure 5The storage unit comprises three sections, with three lifting chip compartments 401 designated as Lifting Chip Compartment 1 4011, Lifting Chip Compartment 2 4012, and Lifting Chip Compartment 3 4013. Each lifting chip compartment can vertically stack 20 microfluidic chips 80. The corresponding sixth Z-axis linear motion mechanisms for each lifting chip compartment 401 are Sixth Z-axis Linear Motion Mechanism 1 4021, Sixth Z-axis Linear Motion Mechanism 2 4022, and Sixth Z-axis Linear Motion Mechanism 3 4023. The first lifting chip compartment 4011 can be driven by the first sixth Z-axis linear motion mechanism 4021 to complete the linear lifting motion along the Z-axis. The second lifting chip compartment 4012 can be driven by the second sixth Z-axis linear motion mechanism 4022 to complete the linear lifting motion along the Z-axis. The third lifting chip compartment 4013 can be driven by the third sixth Z-axis linear motion mechanism 4023 to complete the linear lifting motion along the Z-axis. Together with the first X-axis chip pusher assembly 703, the microfluidic chips 80 in each compartment are transferred to the chip processing module 20.

[0098] like Figures 5 to 7 As shown, when the microfluidic chip 80 in the No. 1 lifting chip compartment 4011, No. 2 lifting chip compartment 4012, and No. 3 lifting chip compartment 4013 needs to be transferred to the chip processing module 20, the No. 1 lifting chip compartment 4011 is driven to rise to a designated height by the No. 1 sixth Z-axis linear motion mechanism 4021, while the No. 2 lifting chip compartment 4012 and No. 3 lifting chip compartment 4013 remain at their lowest positions. The microfluidic chip 80 in the No. 1 lifting chip compartment 4011 is pushed to the chip reheating chamber assembly 201 of the chip processing module 20 by the first X-axis chip pusher assembly 703. When the microfluidic chip 80 in the No. 2 lifting chip compartment 4012 needs to be transferred, the No. 2 lifting chip compartment 4012 is driven to rise to a designated position by the No. 2 sixth Z-axis linear motion mechanism 4022, while the No. 1 lifting chip compartment 4011 is driven to rise to a designated position by the No. 1 sixth Z-axis linear motion mechanism 4022. Mechanism 4021 lowers the chip to its lowest position, while the third lifting chip compartment 4013 remains at its lowest position. The microfluidic chip 80 in the second lifting chip compartment 4012 is pushed to the chip reheating compartment 201 of the chip processing module 20 via the first X-axis chip pusher assembly 703. When the microfluidic chip 80 in the third lifting chip compartment 4013 needs to be transferred, the third lifting chip compartment 4013 is raised to a designated position via the third sixth Z-axis linear motion mechanism 4023, while the second lifting chip compartment 4012 is lowered to its lowest position via the second sixth Z-axis linear motion mechanism 4022. The first lifting chip compartment 4011 remains at its lowest position, while the microfluidic chip 80 in the third lifting chip compartment 4013 is pushed to the chip reheating compartment 201 of the chip processing module 20 via the first X-axis chip pusher assembly 703.

[0099] like Figure 7As shown, there can be three refrigeration components, namely refrigeration component 1 4101, refrigeration component 2 4102 and refrigeration component 3 4103.

[0100] like Figure 8 As shown, the support base 507 is equipped with three chip limiting rods, which are spaced apart along the extension direction of the guide rail 501. These are designated chip limiting rod 503 (first chip limiting rod), 504 (second chip limiting rod), and 505 (third chip limiting rod). The collection position where chip limiting rod 503 is located is used to collect unused microfluidic chips 80, while the collection positions where chip limiting rods 504 and 505 are located are used to collect microfluidic chips 80 after the experiment. The microfluidic chip 80 can be pushed to a designated position along a pair of guide rails 501 by the second X-axis chip pusher assembly 702. The guide rails 501 are then switched from a first state to a second state by the guide rail opening drive mechanism 502, causing the microfluidic chip 80 to fall into the designated collection position. Finally, the operator can manually remove the support base 507 to process the collected microfluidic chip 80.

[0101] The fully automated blood typing analyzer described above has different workflows for different blood typing analysis items. The first and second workflows of the fully automated blood typing analyzer described above will be described in detail below.

[0102] The microfluidic chip 80 mentioned below is a blood typing chip. It can be a slicing centrifugal microfluidic chip, including a tray and a plate, each plate having a sample application port and a reaction port.

[0103] The first working process of the fully automated blood typing analyzer described above includes the following steps:

[0104] S10, the microfluidic chip 80 in the new chip storage module 40 is pushed into the chip warming chamber component 201 of the chip processing module 20 by the first X-axis chip pusher component 703.

[0105] Specifically, the sixth Z-axis linear motion mechanism drives the lifting chip compartment 401 to rise along the Z-axis, so that the microfluidic chip 80 to be pushed is moved onto the moving path of the first X-axis chip pusher; at the same time, the first Z-axis linear drive mechanism 217 moves the first multi-layer chip storage mechanism 220 along the Z-axis, so that the chip receiving part of the microfluidic chip 80 is also moved onto the moving path of the first X-axis chip pusher, that is, aligned with the first X-axis chip pusher; then, the first X-axis chip pusher contacts and pushes the microfluidic chip 80 to the chip receiving part.

[0106] S20, the microfluidic chip 80 is placed in the chip warming chamber assembly 201 for a certain period of time to warm up;

[0107] S30, the Y-axis chip pusher of the Y-axis chip pusher assembly 701 pushes the reheated microfluidic chip 80 onto the first lifting chip tray 210 in the chip positioning centrifugal assembly 202, which is in the chip loading position; then, the first lifting chip tray 210 descends to the chip loading position, the microfluidic chip 80 separates from the first lifting chip tray 210 and is circumferentially limited and sleeved on the outside of the rotating body fixing seat of the chip positioning centrifugal assembly 202 so as to rotate with the rotating body fixing seat;

[0108] S40, the chip-positionable centrifugal assembly 202 drives each disk of the microfluidic chip 80 to rotate to the designated position in sequence; then, the sample loading arm module 30 sequentially adds the sample and reagent pre-loaded in the sample reagent chamber module 10 into the sample loading hole in the current disk;

[0109] S50, control the first lifting chip tray 210 to rise to the chip loading position, so that the microfluidic chip 80 after sample and / or reagent addition is automatically unloaded from the rotating body fixing seat of the chip positioning centrifuge assembly 202; the Y-axis chip pusher of the Y-axis chip pusher assembly 701 contacts and pushes the unloaded microfluidic chip 80 to the second lifting chip tray 211 of the chip centrifuge assembly 203;

[0110] S60, the second lifting chip tray 211 is lowered to the chip loading position, the microfluidic chip 80 is separated from the second lifting chip tray 211 and circumferentially limited and fitted onto the rotating body fixing seat of the chip centrifuge assembly 203, realizing its loading onto the rotating body fixing seat of the chip centrifuge assembly 203; then, the output shaft of the high-speed centrifuge drive mechanism 215 rotates to drive the corresponding rotating body fixing seat to rotate, so that the microfluidic chip 80 rotates and centrifuges at high speed with the rotating body fixing seat, centrifuging the reagents and samples into the reaction well; after a certain period of centrifugation, the reacted sample is driven to the side wall of the reaction well under the action of centrifugal force;

[0111] S70, after centrifugation, the second lifting chip tray 211 is raised to the chip loading position, so that the microfluidic chip 80 is unloaded from the rotating body fixed seat of the chip centrifugation assembly 203; then, the centrifuged microfluidic chip 80 is pushed into the second multilayer chip storage mechanism 221 of the chip reaction assembly 204 by the Y-axis chip pusher assembly 701 for static reaction;

[0112] S80, after the reaction is completed, the microfluidic chip 80, after being left to stand, is pushed onto the third lifting chip tray 212 of the imaging detection component 205 by the second X-axis chip pusher assembly 702. Then, the third lifting chip tray 212 is lowered to the chip loading position, and the microfluidic chip 80 is loaded onto the rotating fixed seat of the imaging detection component 205 for driving by the rotation drive mechanism 216. Then, the output shaft of the rotation drive mechanism 216 rotates, causing the corresponding rotating fixed seat and the microfluidic chip 80 to rotate. The imaging mechanism 209 takes pictures of each reaction hole in the microfluidic chip 80 one by one, and uploads the pictures to the touch screen connected to the device for result analysis. After the detection is completed, the output shaft of the rotation drive mechanism 216 is stopped, and the third lifting chip tray 212 is raised to the chip loading position, so that the microfluidic chip 80 after the detection is completed is unloaded from the corresponding rotating fixed seat.

[0113] S90, the microfluidic chip 80 after detection is pushed onto the guide rail 501 of the chip recycling bin module by the second X-axis chip pusher assembly 702; the guide rail 501 is driven to switch from a first state of mutual proximity to a second state of mutual separation and opening by the guide rail opening drive mechanism 502, so that the microfluidic chip 80 falls into the chip collection device.

[0114] Compared to the first workflow, the specific processes of S40-S60 in the second workflow are different, while the remaining steps are the same. S40-S60 in the second workflow are as follows:

[0115] S40, the chip-positionable centrifugal assembly 202 drives each disk of the microfluidic chip 80 to rotate to the designated position in sequence, and at the same time, the first dispensing needle assembly 301 of the sample dispensing arm module 30 dispenses reagent 1 from the sample reagent chamber module 10 into the dispensing hole in the current disk.

[0116] Subsequently, the first rotary motion mechanism 214 drives the rotating body fixed seat to rotate, and the microfluidic chip 80 rotates at high speed with the rotating body fixed seat, driving the reagent one into the corresponding reaction well through centrifugal force;

[0117] Subsequently, the chip-positionable centrifugation component 202 drives each disk of the microfluidic chip 80 to rotate to the designated position in sequence, while the second dispensing needle component 302 of the sample dispensing arm module 30 dispenses the sample from the sample reagent chamber module 10 into the dispensing hole in the current disk.

[0118] Then, the output shaft of the first rotary motion mechanism 214 drives the rotating body fixed seat to rotate again, and the microfluidic chip 80 rotates at high speed with the rotating body fixed seat, driving the sample into the reaction well through centrifugal force;

[0119] At the same time, the first injection needle assembly 301 and the second injection needle assembly 302 move to the needle washing mechanism 305 under the drive of the first X-axis linear motion mechanism 303 and the second X-axis linear motion mechanism 304, respectively, to complete the cleaning.

[0120] Next, the sample dispensing arm module 30 sequentially dispenses reagent two from the sample reagent compartment module 10 into the corresponding dispensing holes of the microfluidic chip 80; the output shaft of the first rotary motion mechanism 214 drives the rotating body fixed seat to rotate again, and the microfluidic chip 80 rotates at high speed with the rotating body fixed seat, driving reagent two into the reaction hole through centrifugal force;

[0121] Finally, the first lifting chip tray 210 rises to the chip loading position, so that the microfluidic chip 80, which has completed the addition of samples and reagents, is unloaded from the rotating body fixing seat of the chip positioning centrifuge assembly 202; after unloading, the first lifting chip tray 210 is heated to a specified temperature to heat and incubate the microfluidic chip 80 it carries.

[0122] S50, the unloaded microfluidic chip 80 is transferred to the second lifting chip tray 211 of the chip centrifugation assembly 203 by the Y-axis chip pusher assembly 701.

[0123] S60, the microfluidic chip 80 is loaded onto the rotating body fixture of the chip centrifuge assembly 203 via the second lifting chip tray 211; then, the output shaft of the high-speed centrifuge drive mechanism 215 rotates to drive the corresponding rotating body fixture to rotate, and the microfluidic chip 80 rotates and centrifuges at high speed with the rotating body fixture for a certain period of time to ensure that the sample after the reaction can adhere tightly to the side wall of the reaction well through the centrifugal force.

[0124] This invention provides a concept and method for a fully automated blood typing analyzer based on a centrifugal microfluidic chip. Many methods and approaches exist for implementing this technical solution; the above are merely preferred embodiments. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of this invention, and these improvements and modifications should also be considered within the scope of protection of this invention. All components not explicitly stated in this embodiment can be implemented using existing technologies.

Claims

1. A fully automated blood typing analyzer based on a centrifugal microfluidic chip, characterized in that, Includes a base plate (602), on which a sample reagent compartment module (10), a chip processing module (20), a new chip storage compartment module (40), a chip recycling compartment module (50), a sample dispensing arm module (30), and a chip pusher module (70) are provided; The chip pusher module (70) is used to contact and push the microfluidic chip (80) from the new chip storage module (40) to the chip processing module (20) for processing, and to push the microfluidic chip (80) after being processed by the chip processing module (20) to the chip recycling module (50) for recycling; The sample loading arm module (30) is located on top of the sample reagent chamber module (10) and the chip processing module (20), and is used to add the sample or reagent in the sample reagent chamber module (10) into the microfluidic chip (80) in the chip processing module (20).

2. The fully automated blood typing analyzer based on a centrifugal microfluidic chip according to claim 1, characterized in that, The chip processing module (20) includes a chip reheating chamber assembly (201), a chip positioning centrifugation assembly (202), a chip centrifugation assembly (203), a chip reaction assembly (204), and an imaging detection assembly (205) arranged sequentially along the chip transfer direction. The sample loading arm module (30) is located between the sample reagent compartment module (10) and the chip-positionable centrifugation component (202); The chip positioning centrifugation assembly (202), the chip centrifugation assembly (203), and the imaging detection assembly (205) each include at least a rotary motion mechanism, a rotating body fixing seat, and a lifting chip tray. The rotary motion mechanism is disposed on the base plate (602), and the rotating body fixing seat is connected to the output shaft of the rotary motion mechanism. The lifting chip tray can be vertically and vertically disposed on the base plate (602) between the chip loading position and the chip mounting position along the Z-axis. When the lifting chip tray is in the chip loading position, the microfluidic chip (80) it carries is located in the chip pusher mold. On the moving path of block (70), at this time, the chip pusher module (70) can push the microfluidic chip (80) currently carried by the lifting chip tray to the next station or push the microfluidic chip (80) of the previous station into the current lifting chip tray. The pushed-in microfluidic chip (80) is supported on the lifting chip tray and can move with the lifting chip tray. When the lifting chip tray descends to the chip loading position, the microfluidic chip (80) separates from the lifting chip tray and is circumferentially limited and sleeved on the outside of the rotating body fixing seat so as to rotate with the rotating body fixing seat.

3. The fully automated blood typing analyzer based on a centrifugal microfluidic chip according to claim 2, characterized in that, The chip reheating chamber assembly (201), the chip positioning centrifugation assembly (202), the chip centrifugation assembly (203), and the chip reaction assembly (204) are arranged sequentially along the Y-axis; the chip reaction assembly (204), the imaging detection assembly (205), and the chip recovery chamber module (50) are arranged sequentially along the X-axis. The chip pusher module (70) includes a first X-axis chip pusher assembly (703), a second X-axis chip pusher assembly (702), and a Y-axis chip pusher assembly (701) that are respectively directly or indirectly mounted on the base plate (602). The first X-axis chip pusher assembly (703) is provided with a first X-axis chip pusher. The first X-axis chip pusher moves in a straight line along the X-axis direction to push the new microfluidic chip (80) from the new chip storage module (40) to the chip warming chamber assembly (201). The Y-axis chip pusher assembly (701) is equipped with a Y-axis chip pusher, which makes a linear reciprocating motion along the Y-axis direction to push out the microfluidic chip (80) in the chip warming chamber assembly (201) and pass through the chip positioning centrifuge assembly (202), the chip centrifuge assembly (203) and the chip reaction assembly (204) in sequence. The second X-axis chip pusher assembly (702) is provided with a second X-axis chip pusher. The second X-axis chip pusher moves in a straight line along the X-axis direction to push out the microfluidic chip (80) in the chip reaction assembly (204) and pass through the imaging detection assembly (205) and the chip recycling bin module (50) in sequence.

4. The fully automated blood typing analyzer based on a centrifugal microfluidic chip according to claim 3, characterized in that, The chip warming chamber assembly (201) includes: The first multi-layer chip storage mechanism (220) has multiple chip accommodating parts arranged along the Z-axis direction; each chip accommodating part is used to accommodate a microfluidic chip (80); the chip accommodating part has openings on both sides along the Y-axis direction corresponding to the Y-axis chip pusher of the Y-axis chip pusher assembly (701) and the chip positioning centrifugal assembly (202), and has an opening on one side along the X-axis direction corresponding to the first X-axis chip pusher of the first X-axis chip pusher assembly (703); And a first Z-axis linear drive mechanism (217) is directly or indirectly mounted on the base plate (602) and connected to the first multi-layer chip storage mechanism (220) to drive the first multi-layer chip storage mechanism (220) to move linearly back and forth along the Z-axis direction; When the chip receiving portion of the chip to be received moves to the moving path of the first X-axis chip pusher assembly (703), the new microfluidic chip (80) is pushed from the new chip storage module (40) into the chip receiving portion of the chip to be received by the first X-axis chip pusher of the first X-axis chip pusher assembly (703); when the chip receiving portion of the chip to be transferred moves to the moving path of the Y-axis chip pusher assembly (701), the warmed microfluidic chip (80) is pushed into the chip positioning centrifuge assembly (202) by the Y-axis chip pusher of the Y-axis chip pusher assembly (701).

5. The fully automated blood typing analyzer based on a centrifugal microfluidic chip according to claim 3, characterized in that, The chip-positionable centrifugal assembly (202) also includes a QR code scanning mechanism (213), and the rotational motion mechanism in the chip-positionable centrifugal assembly (202) is a first rotational motion mechanism (214); the QR code scanning mechanism (213) and the first rotational motion mechanism (214) work together to complete the scanning of the QR code on the surface of the microfluidic chip (80).

6. The fully automated blood typing analyzer based on a centrifugal microfluidic chip according to claim 3, characterized in that, The chip reaction assembly (204) includes a second multi-layer chip storage mechanism (221) and a third Z-axis linear drive mechanism (218). The second multi-layer chip storage mechanism (221) is used to receive and hold the microfluidic chip (80) from the chip centrifugation assembly (203). The third Z-axis linear drive mechanism (218) is configured to drive the second multi-layer chip storage mechanism (221) to move linearly back and forth along the Z-axis direction, and cooperate with the Y-axis chip pusher assembly (701) to transfer the held microfluidic chip (80) to the imaging detection assembly (205).

7. The fully automated blood typing analyzer based on a centrifugal microfluidic chip according to claim 3, characterized in that, The imaging detection component (205) also includes an imaging mechanism (209), and the rotational motion mechanism in the imaging detection component (205) is a rotational drive mechanism (216); the rotational drive mechanism (216) can drive the microfluidic chip (80) loaded on it to rotate at any position, and cooperate with the imaging mechanism (209) to complete the imaging detection of each reaction hole in the microfluidic chip (80); after the detection is completed, the microfluidic chip (80) is transferred to the chip recycling bin module (50) under the drive of the second X-axis chip pusher of the second X-axis chip pusher component (702).

8. The fully automated blood typing analyzer based on a centrifugal microfluidic chip according to claim 3, characterized in that, The new chip storage module (40) includes one or more storage units arranged side by side along the X-axis. Each storage unit includes a cabinet (406), a lifting chip compartment (401), and a sixth Z-axis linear motion mechanism corresponding to each lifting chip compartment (401). The lifting chip compartment (401) has a accommodating space for storing multiple vertically stacked microfluidic chips (80). When the lifting chip compartment (401) is in the lowered position, the lifting chip compartment (401) and the cabinet (406) enclose the accommodating space, and the tops of all the lifting chip compartments (401) and the cabinet (406) together form a chip delivery channel. Each of the sixth Z-axis linear motion mechanisms is installed on the corresponding cabinet (406), and each lifting chip compartment (401) is connected to the corresponding sixth Z-axis linear motion mechanism. Each of the sixth Z-axis linear motion mechanisms is configured to drive the corresponding lifting chip compartment (401) to perform linear lifting motion along the Z-axis. The first X-axis chip pusher assembly (703) is disposed on one side of the chip transport channel; the sixth Z-axis linear motion mechanism works together with the first X-axis chip pusher assembly (703) to transfer the microfluidic chip (80) of the lifting chip compartment (401) along the chip transport channel to the chip processing module (20).

9. The fully automated blood typing analyzer based on a centrifugal microfluidic chip according to claim 3, characterized in that, The chip recycling bin module (50) includes: The outer frame of the recycling bin (508) has a pull-out space (509) inside. A chip collection device for collecting used microfluidic chips (80) is provided inside the outer frame (508) of the recycling bin, which can be pulled out of the pull-out space (509) and retracted from the pull-out space (509); a chip drop outlet (510) is provided at the top of the pull-out space (509) corresponding to the position of the chip collection device in the retracted position. A pair of guide rails (501) are disposed above the chip drop port (510). The pair of guide rails (501) have a first state in which they are close to each other to carry the used microfluidic chip (80) from the chip processing module (20) and a second state in which they are far apart and spread out so that the used microfluidic chip (80) carried by them falls through the chip drop port (510) into the chip collection device. And a guide rail opening drive mechanism (502) for driving the pair of guide rails (501) to move closer to each other and further away from each other, the guide rail opening drive mechanism (502) is mounted on the base plate (602) through the outer frame (508) of the recycling bin; the pair of guide rails (501) are connected to the guide rail opening drive mechanism (502).

10. The fully automated blood typing analyzer based on a centrifugal microfluidic chip according to claim 3, characterized in that, The sample reagent compartment module (10) includes: A reagent rotary mixing mechanism (101) for loading one or more reagents to be mixed. A reagent loading mechanism (102) for loading one or more reagents that do not require mixing. Sample rack mechanism (103) for loading multiple sampling tubes; The first Y-axis linear drive mechanism (104) is directly or indirectly mounted on the base plate (602); the reagent rotation mixing mechanism (101) and the reagent loading mechanism (102) are respectively connected to the first Y-axis linear drive mechanism (104), and the first Y-axis linear drive mechanism (104) is configured to drive the reagent rotation mixing mechanism (101) and the reagent loading mechanism (102) to reciprocate synchronously along the Y-axis direction; And a sample rack pusher mechanism (105) is directly or indirectly installed on the base plate (602) and connected to the sample rack mechanism (103). The sample rack pusher mechanism (105) is configured to drive the sample rack mechanism (103) to reciprocate along the Y-axis. The sample loading arm module (30) includes: First injection needle assembly (301); Second injection needle assembly (302); The second mounting bracket (306) is fixedly mounted on the base plate (602); The first X-axis linear motion mechanism (303) and the second X-axis linear motion mechanism (304) are respectively mounted on the second mounting bracket (306) at intervals. The fourth Z-axis linear motion mechanism (309) is mounted on the first X-axis linear motion mechanism (303) and connected to the first injection needle assembly (301); The fifth Z-axis linear motion mechanism (308) is mounted on the second X-axis linear motion mechanism (304) and connected to the second injection needle assembly (302); The first injection needle cleaning position (3051) and the second injection needle cleaning position (3052) are located on the movement path of the first injection needle assembly (301) and the second injection needle cleaning position (3052) are located on the movement path of the second injection needle assembly (302).

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