Fully-automatic blood type analyzer based on centrifugal microfluidic chip

Abstract

The application discloses a kind of based on centrifugal microfluidic chip's full-automatic blood type analyzer, belong to blood type analysis equipment technical field.The analyzer includes bottom plate, and sample reagent bin module is arranged on bottom plate, chip processing module, new chip storage bin module, chip recycling bin module, sample adding arm module and chip pusher module;Chip pusher module is used to contact and promote microfluidic chip from new chip storage bin module translation to chip processing module processing, and promote after microfluidic chip translation to chip recycling bin module recycling after chip processing module processing;Sample adding arm module is located at the top of sample reagent bin module and chip processing module, for adding sample or reagent.The application is based on centrifugal microfluidic chip, realizes full-automatic sample and reagent addition, chip transfer, chip loading, chip centrifugation, result detection judgment and chip recycling, without manual intervention, support sample no shutdown sample, sample into result, smaller volume under same test speed.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of detection, in particular to a full-automatic blood type analyzer based on a centrifugal microfluidic chip. BACKGROUND

[0002] With the continuous development of clinical science and the continuous in-depth study of blood type, blood type detection is more and more indispensable, which provides an exact basis for clinical treatment and diagnosis. It is commonly used for blood type identification before clinical blood transfusion treatment, such as trauma, surgery, severe anemia, hemorrhagic disease (hemophilia) and tumor chemotherapy, etc. blood transfusion treatment, which provides a basis for compatible blood transfusion and is an important guarantee for safe blood transfusion. Neonatal hemolytic disease is an important cause of neonatal death in clinic, which can cause fetal development arrest, deformity, abortion and neonatal hyperbilirubinemia. Blood type detection can predict the possibility and severity of neonatal hemolytic disease in the fetus as soon as possible, and provide a basis for the prevention and diagnosis of neonatal hemolytic disease. In addition, blood type detection can also be applied to organ transplantation, forensic identification and other fields.

[0003] The methods and forms of blood type detection are various, such as serology, gene detection blood type, etc. From the comprehensive comparison of economy, efficiency and detection accuracy, serological blood type detection is the most accurate and most commonly used method in clinical medicine. Traditional manual and semi-automatic blood type experiment method not only has complicated operation, low efficiency and poor precision, but also is influenced by the working experience of the operator, and is more prone to human error, which cannot meet the increasing inspection demand of medical institutions. The full-automatic blood type analyzer can well solve these problems.

[0004] At present, the full-automatic blood type analyzers on the market are mainly card type (microcolumn gel method) and plate type (microplate method). No matter which device, due to the flat loading method of consumables and the limitation of methodology, in order to achieve high-throughput speed requirement, the device volume has to be increased, which is a pain point for most medical institutions' inspection departments. And the device is complex and has high maintenance cost.

[0005] The information disclosed in this BACKGROUND section is only intended to increase an understanding of the general context in which the present application can be practiced. It is not admitted that any of the information provided in this section constitutes prior art that is already known in the art. SUMMARY

[0006] The present application aims to solve the technical problems of the prior art, and provides a full-automatic blood type analyzer based on a centrifugal microfluidic chip, which has small device volume, is a desktop device, and occupies small space.

[0007] In order to solve the above technical problems, the application discloses a kind of full-automatic blood type analyzers based on centrifugal microfluidic chip, including bottom plate, sample reagent bin module is arranged on the bottom plate, chip processing module, new chip storage bin module, chip recycling bin module, sample arm module and chip pusher module;

[0008] The chip pusher module is used to contact and push the microfluidic chip to translate from the new chip storage bin module to the chip processing module for processing, and to push the microfluidic chip after processing in the chip processing module to the chip recycling bin module for recycling.

[0009] The sample arm module is located at the top of the sample reagent bin module and the chip processing module, and is used to add the sample or reagent in the sample reagent bin module into the microfluidic chip in the chip processing module.

[0010] Specifically, the chip processing module includes a chip rewarming bin assembly, a chip positionable centrifugation assembly, a chip centrifugation assembly, a chip reaction assembly, and an imaging detection assembly arranged in sequence along the chip transfer direction; the chip rewarming bin assembly is located between the chip positionable centrifugation assembly and the new chip storage bin module; the imaging detection assembly is located between the chip reaction assembly and the chip recycling bin module; the sample arm module is arranged between the sample reagent bin module and the chip positionable centrifugation assembly.

[0011] The chip positionable centrifugation assembly, the chip centrifugation assembly, and the imaging detection assembly each include at least one rotating motion mechanism, one rotating body fixing seat, and one lifting chip tray; the rotating motion mechanism is arranged on the bottom plate; the rotating body fixing seat is connected with the output shaft of the rotating motion mechanism; the lifting chip tray can be lifted and lowered along the Z-axis direction between a chip loading position and a chip loading position on the bottom plate; when the lifting chip tray is at the chip loading position, the microfluidic chip carried by the lifting chip tray is located on the moving path of the chip pusher module; at this time, the chip pusher module can push the microfluidic chip carried by the current lifting chip tray to the next station or push the microfluidic chip in the previous station into the current lifting chip tray; the pushed microfluidic chip is supported on the lifting chip tray and can move with the lifting chip tray; when the lifting chip tray is lowered to the chip loading position, the microfluidic chip is separated from the lifting chip tray and is circumferentially limited and sleeved outside the rotating body fixing seat to rotate with the rotating body fixing seat.

[0012] More specifically, the chip rewarming bin assembly, the chip positionable centrifuging assembly, the chip centrifuging assembly, and the chip reaction assembly are arranged in sequence along the Y-axis direction; the chip reaction assembly, the imaging detection assembly, and the chip recycling bin module are arranged in sequence along the X-axis direction.

[0013] The chip pusher module comprises a first X-axis chip pusher assembly, a second X-axis chip pusher assembly, and a Y-axis chip pusher assembly, which are directly or indirectly mounted on the bottom plate respectively;

[0014] The first X-axis chip pusher assembly is provided with a first X-axis chip pusher, which moves linearly and reciprocally along the X-axis direction, and is used for pushing a new microfluidic chip from the new chip storage bin module to the chip rewarming bin assembly;

[0015] The Y-axis chip pusher assembly is provided with a Y-axis chip pusher, which moves linearly and reciprocally along the Y-axis direction, and is used for pushing the microfluidic chip in the chip rewarming bin assembly out and sequentially through the chip positionable centrifuging assembly, the chip centrifuging assembly, and the chip reaction assembly;

[0016] The second X-axis chip pusher assembly is provided with a second X-axis chip pusher, which moves linearly and reciprocally along the X-axis direction, and is used for pushing the microfluidic chip in the chip reaction assembly out and sequentially through the imaging detection assembly and the chip recycling bin module.

[0017] Specifically, the chip rewarming bin assembly comprises:

[0018] A first multi-layer chip storage mechanism is provided with a plurality of chip accommodating portions along the Z-axis direction; each chip accommodating portion is used for accommodating a microfluidic chip; the chip accommodating portions are provided with openings corresponding to the Y-axis chip pusher of the Y-axis chip pusher assembly and the chip positionable centrifuging assembly on both sides along the Y-axis direction, and are provided with an opening corresponding to the first X-axis chip pusher of the first X-axis chip pusher assembly on one side along the X-axis direction;

[0019] and a first Z-axis linear driving mechanism, which is directly or indirectly mounted on the bottom plate and connected with the first multi-layer chip storage mechanism, and is used for driving the first multi-layer chip storage mechanism to move linearly and reciprocally along the Z-axis direction;

[0020] When the chip accommodating portion of the to-be-received chip moves to the moving path of the first X-axis chip pusher assembly, a new microfluidic chip is pushed into the chip accommodating portion of the to-be-received chip by the first X-axis chip pusher of the first X-axis chip pusher assembly; when the chip accommodating portion of the to-be-transferred chip moves to the moving path of the Y-axis chip pusher assembly, the microfluidic chip after rewarming is pushed into the chip positionable centrifugal assembly by the Y-axis chip pusher of the Y-axis chip pusher assembly.

[0021] Further, the chip positionable centrifugal assembly further comprises a two-dimensional code scanning mechanism, and the rotating motion mechanism in the chip positionable centrifugal assembly is a first rotating motion mechanism; the two-dimensional code scanning mechanism cooperates with the first rotating motion mechanism to complete scanning of the two-dimensional code on the surface of the microfluidic chip.

[0022] Specifically, the chip reaction assembly comprises a second multilayer chip storage mechanism and a third Z-axis linear drive mechanism, the second multilayer chip storage mechanism is used for receiving and standing the microfluidic chip from the chip centrifugal assembly, and the third Z-axis linear drive mechanism is configured to drive the second multilayer chip storage mechanism to move linearly along the Z-axis direction reciprocally, and cooperate with the Y-axis chip pusher assembly to transfer the microfluidic chip after standing reaction to the imaging detection assembly.

[0023] Further, the imaging detection assembly further comprises an imaging mechanism, and the rotating motion mechanism in the imaging detection assembly is a rotating drive mechanism; the rotating drive mechanism can drive the microfluidic chip loaded thereon to rotate at any position, cooperate with the imaging mechanism to complete photographing detection of each reaction well in the microfluidic chip; and the microfluidic chip after detection is transferred to the chip recycling bin module under the driving of the second X-axis chip pusher of the second X-axis chip pusher assembly.

[0024] Specifically, the new chip storage bin module comprises one or more storage bin units arranged side by side along the X-axis direction, each storage bin unit comprises a cabinet body, a lifting chip bin and a sixth Z-axis linear motion mechanism corresponding to each lifting chip bin, the lifting chip bin has an accommodating space for storing a plurality of microfluidic chips stacked vertically, when the lifting chip bin is in a lowered position, the lifting chip bin and the cabinet body enclose the accommodating space, and the top of all the lifting chip bins and the cabinet body jointly form a chip conveying channel; each sixth Z-axis linear motion mechanism is installed on the corresponding cabinet body, each lifting chip bin is connected to the corresponding sixth Z-axis linear motion mechanism, and each sixth Z-axis linear motion mechanism is configured to drive the corresponding lifting chip bin to move linearly along the Z-axis direction;

[0025] The first X-axis chip pusher assembly is arranged on one side of the chip conveying channel; the sixth Z-axis linear motion mechanism cooperates with the first X-axis chip pusher assembly to complete the transfer of the microfluidic chip in the lifting chip bin to the chip processing module along the chip conveying channel.

[0026] Specifically, the chip recycling bin module comprises:

[0027] A recycling bin outer frame is arranged inside the pulling space;

[0028] A chip collecting device for collecting used chips is arranged in the recycling bin outer frame in a pullable manner at an extracted position of the pulling space and a retracted position of the pulling space; the top of the pulling space is provided with a chip drop opening corresponding to the position of the chip collecting device in the retracted position;

[0029] A pair of guide rails arranged above the chip drop opening have a first state of approaching each other to carry the used microfluidic chip from the chip processing module and a second state of opening away from each other to make the carried used microfluidic chip drop through the chip drop opening into the chip collecting device;

[0030] And a guide rail opening driving mechanism for driving the pair of guide rails to approach and move away from each other, the guide rail opening driving mechanism is mounted on the bottom plate through the recycling bin outer frame; the pair of guide rails are connected to the guide rail opening driving mechanism.

[0031] Specifically, the sample reagent bin module comprises:

[0032] A reagent rotating mixing mechanism for loading one or more mixed reagents;

[0033] A reagent loading mechanism for loading one or more reagents without mixing;

[0034] A sample rack mechanism for loading a plurality of sample tubes;

[0035] A first Y-axis linear driving mechanism is directly or indirectly mounted on the bottom plate; the reagent rotating mixing mechanism and the reagent loading mechanism are respectively connected to the first Y-axis linear driving mechanism, and the first Y-axis linear driving mechanism is configured to drive the reagent rotating mixing mechanism and the reagent loading mechanism to move reciprocally along the Y-axis direction synchronously;

[0036] And a sample rack pushing mechanism is mounted on the bottom plate and connected to the sample rack mechanism, and the sample rack pushing mechanism is configured to drive the sample rack mechanism to move reciprocally along the Y-axis direction;

[0037] The sample adding arm module comprises:

[0038] a first filling needle assembly;

[0039] a second filling needle assembly;

[0040] a second mounting frame fixedly mounted on the bottom plate;

[0041] a first X-axis linear motion mechanism and a second X-axis linear motion mechanism, which are respectively installed on the second mounting frame in a spaced manner;

[0042] a fourth Z-axis linear motion mechanism installed on the first X-axis linear motion mechanism and connected with the first filling needle assembly;

[0043] a fifth Z-axis linear motion mechanism installed on the second X-axis linear motion mechanism and connected with the second filling needle assembly;

[0044] and a first filling needle cleaning position and a second filling needle cleaning position, the first filling needle cleaning position being located on the moving path of the first filling needle assembly, and the second filling needle cleaning position being located on the moving path of the second filling needle assembly.

[0045] Beneficial effects:

[0046] 1. The centrifugal microfluidic chip-based full-automatic blood type analyzer provided in the application realizes double-needle cooperative sample adding through cooperation of a sample adding arm module and a sample reagent bin module, reduces waiting time, realizes automatic loading of a new microfluidic chip, and sequentially processes, transports and automatically collects the used chip in a chip processing module, so that high-throughput full-automatic analysis is realized without manual intervention, sample loading is supported without stopping, sample is put in, result is out, and the instrument has a smaller size under the same test speed.

[0047] 2. The centrifugal microfluidic chip-based full-automatic blood type analyzer provided in the application integrates a sample reagent bin module, a chip processing module, a sample adding arm module, a new chip storage bin module, a chip recovery bin module and a chip pusher module on a bottom plate to form a desktop device, which is small in size.

[0048] 3. The application adopts double-needle cooperative sample adding, which can reduce the use of TIP consumables, reduce the use cost, simplify the device structure and improve the device working stability compared with the traditional workstation type full-automatic blood type analyzer.

[0049] 4. In the chip processing module of the present application, components that must drive the chip to rotate during processing, such as chip positionable centrifugal components, chip centrifugal components, and imaging detection components, are designed to allow the centrifugal microfluidic chip to be automatically unloaded and loaded by moving up and down relative to the rotating motion mechanism driven by the lifting chip tray, thereby allowing the analyzer of the present application to use a pusher assembly as a chip transfer mechanism. Compared with the suction cup transfer mechanism, using a pusher assembly as a chip transfer mechanism has at least the following advantages: 1) mechanical contact, reliable, not dependent on the cleanliness and flatness of the chip surface; 2) only contacting the edge of the chip, not contacting the top surface of the chip, reducing the risk of contamination; 3) contacting and pushing the chip, compared with the concentrated suction force of the suction cup, suitable for chips with fragile and thin structures; 4) motor driven, no air circuit and sensor required, low cost, small space occupation, suitable for desktop equipment and more tidy inside the equipment; 5) direct pushing, compared with pneumatic, higher efficiency; 6) contacting the side edge of the chip, the chip does not need to reserve an adsorption area, which helps the design and manufacture of the chip itself. BRIEF DESCRIPTION OF DRAWINGS

[0050] The above and / or other aspects of the present application will become apparent and more readily appreciated from the following description, taken in conjunction with the accompanying drawings, in which:

[0051] Figure 1 A perspective view of a full-automatic blood type analyzer based on a centrifugal microfluidic chip is provided for an embodiment of the present application;

[0052] Figure 2 A perspective view of a chip processing module, a second X-axis chip pusher assembly, and a Y-axis chip pusher assembly in the full-automatic blood type analyzer shown in Figure 1

[0053] Figure 3 A perspective view of a sample reagent storage module in the full-automatic blood type analyzer shown in Figure 1

[0054] Figure 4 A perspective view of a sample reagent storage module in the full-automatic blood type analyzer shown in Figure 1

[0055] Figure 5 A perspective view of a new chip storage module in the full-automatic blood type analyzer shown in Figure 1 Figure 1

[0056] Figure 6 A perspective view of a new chip storage module in the full-automatic blood type analyzer shown in Figure 1 Figure 2 A perspective view of a new chip storage module in the full-automatic blood type analyzer shown in​​​​​​

[0057] Figure 7 For Figure 1 The three-dimensional structure of the new chip storage module in the automatic blood type analyzer shown Figure 3 ;

[0058] Figure 8 For Figure 1 The three-dimensional structure of the chip recycling module in the automatic blood type analyzer shown

[0059] Figure 9 For Figure 8 The three-dimensional structure of the guide rail opening drive mechanism in the chip recycling module shown

[0060] Figure 10 For Figure 1 The assembly structure diagram of the bottom plate, power box assembly, water cooling circulating pump, water cooling heat dissipation row, rack body and plunger pump in the automatic blood type analyzer shown

[0061] Explanation of reference signs:

[0062] 10, sample reagent bin module; 101, reagent rotating mixing mechanism; 1011, rotating mixing mechanism; 102, reagent loading mechanism; 1012, mixing driving mechanism; 103, sample rack mechanism; 104, first Y-axis linear driving mechanism; 105, sample rack pushing mechanism; 106, first mounting rack; 20, chip processing module; 201, chip rewarming bin assembly; 202, chip positionable centrifugation assembly; 203, chip centrifugation assembly; 204, chip reaction assembly; 205, imaging detection assembly; 206, fixed support assembly; 209, imaging mechanism; 210, first lifting chip tray; 211, second lifting chip tray; 212, third lifting chip tray; 213, two-dimensional code scanning mechanism; 214, first rotary motion mechanism; 215, high-speed centrifugation driving mechanism; 216, rotary driving mechanism; 217, first Z-axis linear driving mechanism; 218, third Z-axis linear driving mechanism; 219, Y-axis chip pusher driving mechanism; 220, first multi-layer chip storage mechanism; 221, second multi-layer chip storage mechanism; 30, sample adding arm module; 301, first filling needle assembly; 302, second filling needle assembly; 303, first X-axis linear motion mechanism; 304, second X-axis linear motion mechanism; 305, needle washing mechanism; 3051, first filling needle cleaning position; 3052, second filling needle cleaning position; 306, second mounting rack; 308, fifth Z-axis linear motion mechanism; 309, fourth Z-axis linear motion mechanism; 40, new chip storage bin module; 401, lifting chip bin; 4011, No. 1 lifting chip bin; 4012, No. 2 lifting chip bin; 4013, No. 3 lifting chip bin; 4021, No. 1 sixth Z-axis linear motion mechanism; 4022, No. 2 sixth Z-axis linear motion mechanism; 4023, No. 3 sixth Z-axis linear motion mechanism; 406, cabinet body; 4101, No. 1 refrigeration assembly; 4102, No. 2 refrigeration assembly; 4103, No. 3 refrigeration assembly; 411, semiconductor refrigeration sheet; 412, water tank assembly; 50, chip recycling bin module; 501, guide rail; 502, guide rail opening driving mechanism; 5021, driving motor; 5022, transmission synchronous belt; 5023, toothed rod mechanism; 5024, linear guide rail; 5025, first guide rail lever; 5026, second guide rail lever; 503, No. 1 chip limiting rod; 504, No. 2 chip limiting rod; 505, No. 3 chip limiting rod; 507, supporting base; 508, recycling bin outer frame; 509, pulling space; 510, chip falling port; 601, power module; 602, bottom plate; 603, rack body; 604, plunger pump; 605, water-cooling circulating pump; 606, water-cooling heat dissipation row; 607, external switch; 608, external interface; 70, chip pusher module; 701, Y-axis chip pusher assembly; 702, second X-axis chip pusher assembly; 703, first X-axis chip pusher assembly; 80, microfluidic chip. DETAILED DESCRIPTION

[0063] The specific structure of the embodiment of the present application is shown in Figures 1 to 8 In the embodiment, the X-axis, the Y-axis and the Z-axis are perpendicular to each other, the Z-axis is arranged along the vertical direction, and the X-axis and the Y-axis are arranged in the horizontal plane.

[0064] In the present application, the microfluidic chip 80 or the chip refers to a centrifugal microfluidic chip, which can be a split centrifugal microfluidic chip including a disc carrier and a disc chip. The pusher in the present application refers to a chip that is contacted and pushed by the pusher.

[0065] As shown in Figure 1 The full-automatic blood type analyzer based on the centrifugal microfluidic chip in the embodiment includes a bottom plate 602, and the bottom plate 602 is provided with a sample reagent warehouse module 10, a chip processing module 20, a sample adding arm module 30, a new chip storage warehouse module 40, a chip recycling warehouse module 50 and a chip pusher module 70. The new chip storage warehouse module 40 is used to store new microfluidic chips 80; the chip recycling warehouse module 50 is used to store used microfluidic chips 80; the chip pusher module 70 is used to contact and push the microfluidic chips 80 to translate from the new chip storage warehouse module 40 to the chip processing module 20 for processing, and to push the microfluidic chips 80 processed by the chip processing module 20 to translate to the chip recycling warehouse module 50 for recycling; the sample adding arm module 30 is located at the top of the sample reagent warehouse module 10 and the chip processing module 20, and is used to add the sample or reagent in the sample reagent warehouse module 10 into the corresponding microfluidic chip 80 in the chip processing module 20 to meet the needs of blood type analysis.

[0066] As shown in Figure 2 The chip processing module 20 includes a fixed support assembly 206, and a chip rewarming warehouse assembly 201, a chip positionable centrifugation assembly 202, a chip centrifugation assembly 203, a chip reaction assembly 204 and an imaging detection assembly 205 which are fixedly integrated on the bottom plate 602 and arranged in sequence along the chip transfer direction through the fixed support assembly 206. The sample adding arm module 30 is arranged between the sample reagent warehouse module 10 and the chip positionable centrifugation assembly 202, and is used to add the sample or reagent in the sample reagent warehouse module 10 into the microfluidic chip 80 in the chip positionable centrifugation assembly 202 to meet the needs of blood type analysis.

[0067] The chip positionable centrifugal assembly 202, the chip centrifugal assembly 203 and the imaging detection assembly 205 each include at least one rotating motion mechanism, a rotating body fixing seat and a lifting chip tray. The rotating motion mechanism is arranged on the bottom plate 602. The rotating body fixing seat is connected with the output shaft of the rotating motion mechanism. The lifting chip tray can be lifted and lowered along the Z-axis direction on the bottom plate 602 between a chip loading position and a chip unloading position. When the lifting chip tray is at the chip loading position, the microfluidic chip 80 carried thereby is located on the moving path of the chip pusher module. At this time, the chip pusher module 70 can push the microfluidic chip 80 carried by the current lifting chip tray to the next station or push the microfluidic chip 80 in the previous station into the current lifting chip tray. The pushed microfluidic chip 80 is supported on the lifting chip tray and can move with the lifting chip tray. When the lifting chip tray is lowered to the chip loading position, the microfluidic chip 80 is separated from the lifting chip tray and is circumferentially limited and sleeved outside the rotating body fixing seat to rotate with the rotating body fixing seat. The connection structure between the rotating body fixing seat and the microfluidic chip 80 in the embodiment adopts the connection device of the rotating body for medical detection disclosed in the Chinese Utility Model Patent No. CN222510960U. The rotating body fixing seat adopts the rotating body fixing seat in the Chinese Utility Model Patent. The microfluidic chip 80 adopts the structure of the rotating body. When the microfluidic chip 80 is a split-type centrifugal microfluidic chip, the centering guide groove and the limiting groove of the rotating body are formed on the disc holder.

[0068] More specifically, referring to Figure 2 , the chip warming bin assembly 201, the chip positionable centrifugal assembly 202, the chip centrifugal assembly 203 and the chip reaction assembly 204 are arranged in sequence along the Y-axis direction. The chip reaction assembly 204, the imaging detection assembly 205 and the chip recycling bin module 50 are arranged in sequence along the X-axis direction. Referring to Figure 1The chip pusher module 70 includes a first X-axis chip pusher assembly 703, a second X-axis chip pusher assembly 702 and a Y-axis chip pusher assembly 701, which are directly or indirectly mounted on the base plate 602, respectively. The first X-axis chip pusher assembly 703 is provided with a first X-axis chip pusher, which is configured to move linearly along the X-axis direction for pushing the new microfluidic chip 80 from the new chip storage module 40 to the chip rewarming bin assembly 201. The Y-axis chip pusher assembly 701 is provided with a Y-axis chip pusher, which is configured to move linearly along the Y-axis direction for pushing the microfluidic chip 80 in the chip rewarming bin assembly 201 to sequentially pass through the chip positionable centrifugation assembly 202, the chip centrifugation assembly 203 and the chip reaction assembly 204. The second X-axis chip pusher assembly 702 is provided with a second X-axis chip pusher, which is configured to move linearly along the X-axis direction for pushing the microfluidic chip 80 in the chip reaction assembly 204 to sequentially pass through the imaging detection assembly 205 and the chip recycling bin module 50.

[0069] It should be understood that each pusher assembly of the present embodiment includes a pusher driving mechanism for driving the corresponding chip pusher to move linearly and reciprocally, which is directly or indirectly mounted to the base plate 602. The pusher driving mechanism can be a conventional one, and the specific configuration of the pusher driving mechanism is not the main improvement point of the present application, and thus will not be described herein. Figure 2 The Y-axis chip pusher driving mechanism 219 for driving the Y-axis chip pusher to move linearly and reciprocally along the Y-axis direction has been shown.

[0070] In an embodiment, as shown in Figure 3 The sample reagent bin module 10 includes a reagent rotating mixing mechanism 101, a reagent loading mechanism 102, a sample rack mechanism 103, a first Y-axis linear driving mechanism 104, a sample rack pusher mechanism 105 and a first mounting bracket 106. The sample rack mechanism 103 is configured to load a plurality of sample tubes, and the reagent loading mechanism 102 is configured to load one or more reagents that do not need to be mixed. The reagent rotating mixing mechanism 101 is configured to load one or more mixed reagents. The first Y-axis linear driving mechanism 104 and the sample rack pusher mechanism 105 are both mounted to the base plate 602 through the first mounting bracket 106. The reagent rotating mixing mechanism 101 and the reagent loading mechanism 102 are connected to the first Y-axis linear driving mechanism 104, which is configured to drive the reagent rotating mixing mechanism 101 and the reagent loading mechanism 102 to move reciprocally along the Y-axis direction synchronously. The sample rack mechanism 103 is connected to the sample rack pusher mechanism 105, which is configured to drive the sample rack mechanism 103 to move reciprocally along the Y-axis direction.

[0071] As shown in Figure 4As shown, the sample dispensing arm module 30 includes a second mounting bracket 306, a first dispensing needle assembly 301, a second dispensing needle assembly 302, a first X-axis linear motion mechanism 303, a fourth Z-axis linear motion mechanism 309, a second X-axis linear motion mechanism 304, and a fifth Z-axis linear motion mechanism 308. The second mounting bracket 306 is fixedly mounted on the base plate 602. The first X-axis linear motion mechanism 303 and the second X-axis linear motion mechanism 304 are respectively and spaced apart from each other on the second mounting bracket 306. The fourth Z-axis linear motion mechanism 309 is mounted on the first X-axis linear motion mechanism 303. The first dispensing needle assembly 301 is mounted on the fourth Z-axis linear motion mechanism 309. The fifth Z-axis linear motion mechanism 308 is mounted on the second X-axis linear motion mechanism 304. The second dispensing needle assembly 302 is mounted on the fifth Z-axis linear motion mechanism 308.

[0072] In this embodiment, the first injection needle assembly 301 is driven by the first X-axis linear motion mechanism 303 and the fourth Z-axis linear motion mechanism 309 to complete its linear movement and positioning in the X-axis and Z-axis directions, and the second injection needle assembly 302 is driven by the second X-axis linear motion mechanism 304 and the fifth Z-axis linear motion mechanism 308 to complete its linear movement and positioning in the X-axis and Z-axis directions.

[0073] like Figures 2 to 4 As shown, the sample dispensing arm module 30 works in conjunction with the sample reagent compartment module 10 to aspirate any reagent or sample from the reagent rotation mixing mechanism 101, the reagent loading mechanism 102, and the sample rack mechanism 103 via the first dispensing needle assembly 301 and the second dispensing needle assembly 302, and then transfer it to the microfluidic chip 80 loaded within the chip-positionable centrifugation assembly 202. This embodiment achieves dual-needle coordinated sample dispensing through the combined operation of the sample reagent compartment module 10 and the sample dispensing arm module 30, reducing waiting time.

[0074] Specifically, such as Figure 3 As shown, the reagent rotary mixing mechanism 101 includes a rotary mixing mechanism 1011 and a mixing drive mechanism 1012 connected to the rotary mixing mechanism 1011. The rotary mixing mechanism 1011 has multiple mixing positions, and the mixing drive mechanism 1012 is configured to drive the multiple mixing positions of the rotary mixing mechanism 1011 to mix simultaneously. The reagent rotary mixing mechanism 101 can adopt a reagent rotary mixing mechanism in the prior art, and its specific structure is not the main improvement point of this application, so it will not be described in detail here.

[0075] Furthermore, such as Figure 4As shown, the sample adding arm module 30 further comprises a needle washing mechanism 305, which comprises a first needle washing position 3051 located on the moving path of the first needle assembly 301 and a second needle washing position 3052 located on the moving path of the second needle assembly 302. The needle washing mechanism 305 can be mounted on the first mounting frame 106.

[0076] The first needle assembly 301 and the second needle assembly 302 are washed by the needle washing mechanism 305, so as to avoid cross contamination.

[0077] Specifically, as shown, Figure 2 The chip rewarming bin assembly 201 comprises: a first multi-layer chip storage mechanism 220, which is provided with a plurality of chip accommodating portions along the Z-axis direction; each chip accommodating portion is used for accommodating a microfluidic chip 80; the chip accommodating portions are provided with openings corresponding to the Y-axis chip pusher assembly 701 and the chip positionable centrifugation assembly 202 on both sides along the Y-axis direction and are provided with an opening corresponding to the first X-axis chip pusher assembly 703 on one side along the X-axis direction; and a first Z-axis linear drive mechanism 217, which is directly or indirectly mounted on the bottom plate 602 and connected with the first multi-layer chip storage mechanism 220, is used for driving the first multi-layer chip storage mechanism 220 to move linearly and reciprocally along the Z-axis direction; when the chip accommodating portion to be received moves to the moving path of the first X-axis chip pusher assembly 703, the new microfluidic chip 80 is pushed into the chip accommodating portion to be received by the first X-axis chip pusher of the first X-axis chip pusher assembly 703; when the chip accommodating portion to be transferred moves to the moving path of the Y-axis chip pusher assembly 701, the rewarmed microfluidic chip 80 is pushed into the chip positionable centrifugation assembly 202 by the Y-axis chip pusher of the Y-axis chip pusher assembly 701.

[0078] In the present application, rewarming refers to that the chip is placed at room temperature for a period of time after being transferred out of the new chip storage bin module 40, so as to ensure that the liquid reagent in the chip is recovered from a low-temperature state to a room-temperature state, so as to avoid affecting the experimental results.

[0079] Specifically, as shown, Figure 2As shown, the chip positionable centrifugal assembly 202 can position the lifting chip tray in the centrifugal assembly 202 as a first lifting chip tray 210, and the rotating movement mechanism in the chip positionable centrifugal assembly 202 as a first rotating movement mechanism 214. The first lifting chip tray 210 in the chip loading position can receive the microfluidic chip 80 from the chip rewarming bin assembly 201. The microfluidic chip 80 can be loaded onto or unloaded from the first rotating movement mechanism 214 through the first lifting chip tray 210. After loading, the microfluidic chip 80 is driven by the first rotating movement mechanism 214 to complete high-speed centrifugation and can achieve high-precision positioning movement according to requirements.

[0080] Further, the first lifting chip tray 210 can also be configured to be controllably heated to incubate the microfluidic chip 80 carried thereby. Specifically, the controllable heating of the first lifting chip tray 210 is achieved by arranging a heating element such as a thin film heater on the carrying surface of the first lifting chip tray 210.

[0081] Further, the chip positionable centrifugal assembly 202 further comprises a two-dimensional code scanning mechanism 213, which cooperates with the first rotating movement mechanism 214 to complete two-dimensional code scanning on the surface of the microfluidic chip 80 and identify the item information of the microfluidic chip 80.

[0082] Specifically, as shown in Figure 2 The lifting chip tray of the chip centrifugal assembly 203 is a second lifting chip tray 211, and the rotating movement mechanism of the chip centrifugal assembly 203 is a high-speed centrifugal driving mechanism 215. The microfluidic chip 80 can be loaded onto or unloaded from the high-speed centrifugal driving mechanism 215 through the second lifting chip tray 211. The high-speed centrifugal driving mechanism 215 drives the microfluidic chip 80 to complete high-speed centrifugation, which can be transferred to the chip reaction assembly 204 in cooperation with the Y-axis chip pusher assembly 701.

[0083] As shown in Figure 2 The chip reaction assembly 204 comprises a second multi-layer chip storage mechanism 221 and a third Z-axis linear driving mechanism 218. The second multi-layer chip storage mechanism 221 is used to receive and rest the microfluidic chip 80 from the second lifting chip tray 211, and the third Z-axis linear driving mechanism 218 is configured to drive the second multi-layer chip storage mechanism 221 to move linearly along the Z-axis direction. The Y-axis chip pusher assembly 701 transfers the microfluidic chip 80 after resting reaction to the imaging detection assembly 205.

[0084] Specifically, the second multilayer chip storage mechanism 221 can adopt the same structure as the first multilayer chip storage mechanism 220. The difference is that the chip accommodating portion of the second multilayer chip storage mechanism 221 has openings on both sides along the X-axis direction corresponding to the second X-axis chip pusher assembly 702 and the imaging detection assembly 205, and an opening on one side along the Y-axis direction corresponding to the Y-axis chip pusher assembly 701. The third Z-axis linear drive mechanism 218 can drive the second multilayer chip storage mechanism 221 to move linearly back and forth along the Z-axis direction, so that the chip accommodating portion of the chip to be received moves to the moving path of the Y-axis chip pusher assembly 701, so that the Y-axis chip pusher assembly 701 can push the microfluidic chip 80 from the chip centrifugation assembly 203 into the chip accommodating portion of the chip to be received, or move the chip accommodating portion of the chip to be transferred to the moving path of the second X-axis chip pusher assembly 702, so that the second X-axis chip pusher assembly 702 can push the microfluidic chip 80 after static reaction into the imaging detection assembly 205.

[0085] Specifically, such as Figure 2 As shown, the lifting chip tray of the imaging detection component 205 is a third lifting chip tray 212, and the rotation mechanism in the imaging detection component 205 is a rotation drive mechanism 216. The imaging detection component 205 also includes an imaging mechanism 209. The third lifting chip tray 212 is used to receive the microfluidic chip 80 from the chip reaction component 204 and load it onto or unload it from the rotation drive mechanism 216. The rotation drive mechanism 216 can drive the loaded microfluidic chip 80 to rotate to any position, and cooperate with the imaging mechanism 209 to complete the imaging detection of each reaction hole in the microfluidic chip 80. After the detection is completed, the microfluidic chip 80 is transferred to the chip recycling bin module 50 under the drive of the second X-axis chip pusher of the second X-axis chip pusher component 702.

[0086] Specifically, such as Figures 5 to 7As shown, the new chip storage bin module 40 includes one or more storage bin units arranged side by side along the X-axis direction, each storage bin unit including a cabinet body 406, a lifting chip bin 401, and a sixth Z-axis linear motion mechanism corresponding to each lifting chip bin 401, the lifting chip bin 401 having a receiving space for storing a plurality of vertically stacked microfluidic chips 80, when the lifting chip bin 401 is in a lowered position, the lifting chip bin 401 and the cabinet body 406 enclose and close the receiving space, and the top of all lifting chip bins 401 and cabinet bodies 406 collectively form a chip conveying channel; each sixth Z-axis linear motion mechanism is respectively mounted on the corresponding cabinet body 406, each lifting chip bin 401 is respectively connected to the corresponding sixth Z-axis linear motion mechanism, and each sixth Z-axis linear motion mechanism is respectively configured to drive the corresponding lifting chip bin 401 to move linearly along the Z-axis direction. The first X-axis chip pusher assembly 703 is arranged on one side of the chip conveying channel; the sixth Z-axis linear motion mechanism and the first X-axis chip pusher assembly 703 cooperate to complete the transfer of the microfluidic chip 80 of the lifting chip bin 401 along the chip conveying channel to the chip processing module 20.

[0087] Specifically, when one of the lifting chip bins 401 is raised to the topmost microfluidic chip 80, which is located on the pusher moving path of the first X-axis chip pusher assembly 703, the other lifting chip bins 401 are all in a lowered position, at this time, the pusher of the first X-axis chip pusher assembly 703 contacts and pushes the microfluidic chip 80 to transfer along the chip conveying channel to one of the chip receiving portions in the chip processing module 20.

[0088] Referring to Figure 7 and Figure 10 The analyzer further includes a low-temperature refrigeration system, the low-temperature refrigeration system including a refrigeration assembly corresponding to each storage bin unit, a water-cooled circulating pump 605, and a water-cooled heat dissipation row 606, each refrigeration assembly including a semiconductor refrigeration sheet 411 and a water tank assembly 412, referring to Figure 7 The cold end of the semiconductor refrigeration sheet 411 in each refrigeration assembly faces upward and is attached to the bottom of the cabinet body 406, and the water tank assembly 412 is arranged at the hot end of the semiconductor refrigeration sheet 411, and the semiconductor refrigeration sheet 411 generates heat at the hot end when refrigerating the new chip storage bin module 40. The water tank assembly 412, the water-cooled circulating pump 605, and the water-cooled heat dissipation row 606 are connected by pipelines to form a refrigerant circulation loop, and the water-cooled circulating pump 605 drives the refrigerant in the pipeline to flow through each water tank assembly 412 in turn, and finally reaches the water-cooled heat dissipation row 606 and exchanges the heat absorbed by the refrigerant to the outside.

[0089] Specifically, referring to Figure 10The low-temperature refrigeration system further comprises a rack body 603 installed on the bottom plate 602, and the water-cooling circulating pump 605 and the water-cooling heat dissipation row 606 are respectively installed on the rack body 603.

[0090] Specifically, as shown in Figure 10 , the power supply box assembly is installed on the bottom plate 602. More specifically, as shown in Figure 10 , the power supply box assembly comprises a power supply box body 601, an external switch 607 and an external interface 608, the external switch 607 and the external interface 608 are fixed to the power supply box body 601, and the power supply box body 601 can integrate the power supply module and the filter required by the whole machine together, so as to facilitate the wiring and maintenance of the whole machine. The external switch 607 is a power supply interface and a power supply control interface. The external interface 608 is a device communication interface, which is used for device debugging.

[0091] Further, as shown in Figure 10 , the plunger pump 604 is configured to be capable of performing suction and push-out on the liquid in the filling needle of the first filling needle assembly 301 and the second filling needle assembly 302. The plunger pump 604 is installed on the bottom plate 602.

[0092] Specifically, as shown in Figure 1 and Figure 8 , the chip recycling bin module 50 comprises: a recycling bin outer frame 508, an extraction space 509 is arranged inside the recycling bin outer frame 508; a chip collecting device for collecting used chips, the chip collecting device is arranged in the recycling bin outer frame 508 in an extractable manner at an extraction position of the extraction space 509 and a return position of the extraction space 509; a chip falling port 510 is arranged on the top of the extraction space 509 and corresponds to the position of the chip collecting device in the return position; a pair of guide rails 501 arranged above the chip falling port 510, the pair of guide rails 501 has a first state of approaching each other to carry the used microfluidic chip 80 from the chip processing module 20 and a second state of opening away from each other to make the used microfluidic chip 80 carried by the pair of guide rails 501 fall through the chip falling port 510 and fall into the chip collecting device; and a guide rail opening driving mechanism 502 for driving the pair of guide rails 501 to approach each other and move away from each other, the guide rail opening driving mechanism 502 is installed on the bottom plate 602 through the recycling bin outer frame 508; the pair of guide rails 501 is connected to the guide rail opening driving mechanism 502.

[0093] Specifically, referring to Figure 9The rail opening driving mechanism 502 comprises a driving motor 5021, a transmission synchronous belt 5022, a pair of toothed lead screw mechanisms 5023, a linear guide rail 5024, a first rail shifting rod 5025 and a second rail shifting rod 5026. The driving motor 5021, the linear guide rail 5024 and the pair of toothed lead screw mechanisms 5023 are respectively mounted on the recycling chamber outer frame 508, and the first rail shifting rod 5025 and the second rail shifting rod 5026 are respectively threadedly connected to the pair of toothed lead screws of the pair of toothed lead screw mechanisms 5023. One of the pair of rails 501 is connected to the first rail shifting rod 5025, and the other is connected to the second rail shifting rod 5026. The driving motor 5021 and the pair of toothed lead screw mechanisms 5023 are drivingly connected through the transmission synchronous belt 5022 to drive the pair of toothed lead screws of the pair of toothed lead screw mechanisms 5023 to rotate. When the driving motor 5021 drives the pair of toothed lead screws of the pair of toothed lead screw mechanisms 5023 to rotate, the pair of toothed lead screw mechanisms 5023 drive the first rail shifting rod 5025 and the second rail shifting rod 5026 to move linearly towards or away from each other along the linear guide rail 5024. The linear movement direction of the first rail shifting rod 5025 and the second rail shifting rod 5026 is controlled by changing the rotation direction of the driving motor 5021, thereby controlling the mutual approach and mutual separation of the pair of rails 501.

[0094] More specifically, referring to Figure 8 The chip collection device comprises a supporting base 507 for supporting the used microfluidic chip 80, the supporting base 507 is provided with one or more collection positions for stacking the used microfluidic chip 80; the one or more collection positions are arranged at intervals along the extension direction of the guide rail 501; and a chip limiting part for stopping the circumferential displacement of the microfluidic chip 80, each collection position is respectively provided with a corresponding chip limiting part.

[0095] In this embodiment, the first X-axis chip pusher assembly 703 drives the used microfluidic chip 80 to move on the pair of guide rails 501 in the first state. When the used microfluidic chip 80 moves to the position directly above one of the collection positions, the rail opening driving mechanism 502 drives the pair of guide rails 501 to open to the second state, and the used microfluidic chip 80 falls and is stacked above the uppermost microfluidic chip 80 collected in the collection position. The chip limiting part limits the microfluidic chip 80 stacked in the collection position.

[0096] More specifically, referring to Figure 8 The chip limiting part is a chip limiting rod, which is configured to allow the microfluidic chip 80 to be sleeved thereon. The distance between two adjacent chip limiting rods is greater than the outer diameter of the microfluidic chip 80.

[0097] In a specific embodiment, referring to Figure 5The storage bin unit is provided with three, and the lifting chip bins 401 in the three storage bin units are respectively a first lifting chip bin 4011, a second lifting chip bin 4012 and a third lifting chip bin 4013. The accommodation space of each lifting chip bin can vertically stack 20 microfluidic chips 80. The sixth Z-axis linear motion mechanisms corresponding to the lifting chip bins 401 are respectively a first sixth Z-axis linear motion mechanism 4021, a second sixth Z-axis linear motion mechanism 4022 and a third sixth Z-axis linear motion mechanism 4023. The first lifting chip bin 4011 can be driven by the first sixth Z-axis linear motion mechanism 4021 to complete linear lifting movement along the Z-axis direction, the second lifting chip bin 4012 can be driven by the second sixth Z-axis linear motion mechanism 4022 to complete linear lifting movement along the Z-axis direction, and the third lifting chip bin 4013 can be driven by the third sixth Z-axis linear motion mechanism 4023 to complete linear lifting movement along the Z-axis direction. The first X-axis chip pusher assembly 703 is used to transfer the microfluidic chips 80 in each bin to the chip processing module 20.

[0098] As shown in Figures 5 to 7 When the microfluidic chips 80 in the first lifting chip bin 4011, the second lifting chip bin 4012 and the third lifting chip bin 4013 need to be transferred to the chip processing module 20, the first lifting chip bin 4011 is driven by the first sixth Z-axis linear motion mechanism 4021 to rise to a specified height, the second lifting chip bin 4012 and the third lifting chip bin 4013 remain at the lowest position, and the first X-axis chip pusher assembly 703 is used to push the microfluidic chips 80 in the first lifting chip bin 4011 to the chip rewarming bin assembly 201 of the chip processing module 20. When the microfluidic chips 80 in the second lifting chip bin 4012 need to be transferred, the second lifting chip bin 4012 is driven by the second sixth Z-axis linear motion mechanism 4022 to rise to a specified position, the first lifting chip bin 4011 is driven by the first sixth Z-axis linear motion mechanism 4021 to descend to the lowest position, and the third lifting chip bin 4013 remains at the lowest position. The first X-axis chip pusher assembly 703 is used to push the microfluidic chips 80 in the second lifting chip bin 4012 to the chip rewarming bin assembly 201 of the chip processing module 20. When the microfluidic chips 80 in the third lifting chip bin 4013 need to be transferred, the third lifting chip bin 4013 is driven by the third sixth Z-axis linear motion mechanism 4023 to rise to a specified position, the second lifting chip bin 4012 is driven by the second sixth Z-axis linear motion mechanism 4022 to descend to the lowest position, and the first lifting chip bin 4011 remains at the lowest position. The first X-axis chip pusher assembly 703 is used to push the microfluidic chips 80 in the third lifting chip bin 4013 to the chip rewarming bin assembly 201 of the chip processing module 20.

[0099] As shown in Figure 7As shown, there can be three refrigeration components, namely refrigeration component 1 4101, refrigeration component 2 4102 and refrigeration component 3 4103.

[0100] like Figure 8 As shown, the support base 507 is equipped with three chip limiting rods, which are spaced apart along the extension direction of the guide rail 501. These are designated chip limiting rod 503 (first chip limiting rod), 504 (second chip limiting rod), and 505 (third chip limiting rod). The collection position where chip limiting rod 503 is located is used to collect unused microfluidic chips 80, while the collection positions where chip limiting rods 504 and 505 are located are used to collect microfluidic chips 80 after the experiment. The microfluidic chip 80 can be pushed to a designated position along a pair of guide rails 501 by the second X-axis chip pusher assembly 702. The guide rails 501 are then switched from a first state to a second state by the guide rail opening drive mechanism 502, causing the microfluidic chip 80 to fall into the designated collection position. Finally, the operator can manually remove the support base 507 to process the collected microfluidic chip 80.

[0101] The fully automated blood typing analyzer described above has different workflows for different blood typing analysis items. The first and second workflows of the fully automated blood typing analyzer described above will be described in detail below.

[0102] The microfluidic chip 80 mentioned below is a blood typing chip. It can be a slicing centrifugal microfluidic chip, including a tray and a disc, each disc having a sample application port and a reaction port.

[0103] The first working process of the fully automated blood typing analyzer described above includes the following steps:

[0104] S10, the microfluidic chip 80 in the new chip storage module 40 is pushed into the chip warming chamber component 201 of the chip processing module 20 by the first X-axis chip pusher component 703.

[0105] Specifically, the sixth Z-axis linear motion mechanism drives the lifting chip compartment 401 to rise along the Z-axis, so that the microfluidic chip 80 to be pushed is moved onto the moving path of the first X-axis chip pusher; at the same time, the first Z-axis linear drive mechanism 217 moves the first multi-layer chip storage mechanism 220 along the Z-axis, so that the chip receiving part of the microfluidic chip 80 is also moved onto the moving path of the first X-axis chip pusher, that is, aligned with the first X-axis chip pusher; then, the first X-axis chip pusher contacts and pushes the microfluidic chip 80 to the chip receiving part.

[0106] S20, the microfluidic chip 80 is placed in the chip warming chamber assembly 201 for a certain period of time to warm up;

[0107] S30, the Y-axis chip pusher of the Y-axis chip pusher assembly 701 pushes the microfluidic chip 80 after the re-warming to the first lifting chip tray 210 in the chip positionable centrifugal assembly 202 in the chip loading position; then, the first lifting chip tray 210 is lowered to the chip loading position, the microfluidic chip 80 is separated from the first lifting chip tray 210 and is circumferentially limited to be sleeved outside the rotating body fixing seat of the chip positionable centrifugal assembly 202 to rotate with the rotating body fixing seat;

[0108] S40, the chip positionable centrifugal assembly 202 drives each disc of the microfluidic chip 80 to rotate to the designated position in turn; then, the sample and reagent pre-loaded in the sample reagent warehouse module 10 are sequentially added to the sample injection hole in the current disc by the sample arm module 30;

[0109] S50, the first lifting chip tray 210 is controlled to rise to the chip loading position, so that the microfluidic chip 80 after the sample and / or reagent is added is automatically unloaded from the rotating body fixing seat of the chip positionable centrifugal assembly 202; the Y-axis chip pusher of the Y-axis chip pusher assembly 701 contacts and pushes the unloaded microfluidic chip 80 to the second lifting chip tray 211 of the chip centrifugal assembly 203;

[0110] S60, the second lifting chip tray 211 is controlled to be lowered to the chip loading position, the microfluidic chip 80 is separated from the second lifting chip tray 211 and is circumferentially limited to be sleeved on the rotating body fixing seat of the chip centrifugal assembly 203, so as to be loaded on the rotating body fixing seat of the chip centrifugal assembly 203; then, the corresponding rotating body fixing seat is driven to rotate by the output shaft of the high-speed centrifugal driving mechanism 215, so that the microfluidic chip 80 rotates at high speed with the rotating body fixing seat, and the reagent and sample in the microfluidic chip 80 are centrifuged into the reaction hole; after a certain time of centrifugation, the reacted sample is driven to the side wall of the reaction hole under the action of the centrifugal force;

[0111] S70, after the centrifugation is completed, the second lifting chip tray 211 is controlled to rise to the chip loading position, so that the microfluidic chip 80 is unloaded from the rotating body fixing seat of the chip centrifugal assembly 203; then, the microfluidic chip 80 after the centrifugation is pushed by the Y-axis chip pusher of the Y-axis chip pusher assembly 701 to the second multi-layer chip storage mechanism 221 of the chip reaction assembly 204 for static reaction;

[0112] S80, after the reaction is completed, the microfluidic chip 80 after standing reaction is pushed to the third lifting chip tray 212 of the imaging detection assembly 205 by the second X-axis chip pusher of the second X-axis chip pusher assembly 702; then the third lifting chip tray 212 is lowered to the chip loading position, and the microfluidic chip 80 is loaded onto the rotating fixed seat of the imaging detection assembly 205 for driving by the rotating drive mechanism 216; then the rotating fixed seat and the microfluidic chip 80 are rotated by the output shaft of the rotating drive mechanism 216, the imaging mechanism 209 is cooperated to take a photo of each reaction well in the microfluidic chip 80 one by one, and the photo is uploaded to the touch screen connected to the equipment for result analysis; after the detection is completed, the output shaft of the rotating drive mechanism 216 is controlled to stop rotating, and the third lifting chip tray 212 is controlled to rise to the chip loading position, so that the microfluidic chip 80 after the detection is unloaded from the corresponding rotating fixed seat;

[0113] S90, the microfluidic chip 80 after the detection is pushed to the guide rail 501 of the chip recycling bin module by the second X-axis chip pusher of the second X-axis chip pusher assembly 702; the guide rail 501 is switched from the first state of being close to each other to the second state of being away from each other by the guide rail opening drive mechanism 502, so that the microfluidic chip 80 falls into the chip collection device.

[0114] Compared with the first working process, the specific process of S40-S60 in the second working process is different, and the remaining steps are the same. S40-S60 in the second working process are respectively:

[0115] S40, the chip positionable centrifugal assembly 202 drives each disc of the microfluidic chip 80 to rotate to the specified position in turn, and the first injection needle assembly 301 of the sample arm module 30 injects the reagent one in the sample reagent bin module 10 into the sample injection hole in the current disc;

[0116] Subsequently, the rotating fixed seat is rotated by the first rotating motion mechanism 214, the microfluidic chip 80 rotates at high speed with the rotating fixed seat, and the reagent one is driven into the corresponding reaction well by centrifugal force;

[0117] Then, the rotating fixed seat is rotated again by the output shaft of the first rotating motion mechanism 214, the microfluidic chip 80 rotates at high speed with the rotating fixed seat, and the sample is driven into the reaction well by centrifugal force;

[0118] Then, the rotating fixed seat is rotated again by the output shaft of the first rotating motion mechanism 214, the microfluidic chip 80 rotates at high speed with the rotating fixed seat, and the sample is driven into the reaction well by centrifugal force;

[0119] At the same time, the first filling needle assembly 301 and the second filling needle assembly 302 are driven by the first X-axis linear motion mechanism 303 and the second X-axis linear motion mechanism 304 to move to the needle cleaning mechanism 305, and cleaning is completed;

[0120] Then, the sample arm module 30 adds the reagent two in the sample reagent warehouse module 10 to the corresponding sample hole of the microfluidic chip 80 in turn; the output shaft of the first rotary motion mechanism 214 drives the rotary body fixing seat to rotate again, the microfluidic chip 80 rotates with the rotary body fixing seat at high speed, and the reagent two is driven into the reaction hole through centrifugal force;

[0121] Finally, the first lifting chip tray 210 rises to the chip loading position, so that the microfluidic chip 80 after sample addition and reagent addition is unloaded from the rotary body fixing seat of the chip positionable centrifugal assembly 202; after unloading, the first lifting chip tray 210 is controlled to rise to a specified temperature, and the microfluidic chip 80 carried thereby is heated and incubated;

[0122] S50, the unloaded microfluidic chip 80 is transferred to the second lifting chip tray 211 of the chip centrifugal assembly 203 through the Y-axis chip pusher assembly 701.

[0123] S60, the microfluidic chip 80 is loaded onto the rotary body fixing seat of the chip centrifugal assembly 203 through the second lifting chip tray 211; then, the corresponding rotary body fixing seat is driven to rotate by the output shaft of the high-speed centrifugal driving mechanism 215, and the microfluidic chip 80 rotates with the rotary body fixing seat at high speed for a certain time, so that the reacted sample can be tightly attached to the side wall of the reaction hole through the driving of centrifugal force.

[0124] The application provides a kind of based on centrifugal microfluidic chip's full-automatic blood type analyzer's train of thought and method, the method and approach for specifically realizing this technical solution are many, above only is preferred embodiment of the present application, it should be pointed out, for the ordinary skilled person in the art, without departing from the principle of the present application, can make a number of improvements and refinements, these improvements and refinements also should be regarded as the protection scope of the present application. The components not explicitly described in the embodiment can be realized by prior art.

Claims

1. A fully automated blood typing analyzer based on a centrifugal microfluidic chip, characterized in that, Includes a base plate (602), on which a sample reagent compartment module (10), a chip processing module (20), a new chip storage compartment module (40), a chip recycling compartment module (50), a sample dispensing arm module (30), and a chip pusher module (70) are provided; The chip pusher module (70) is used to contact and push the microfluidic chip (80) from the new chip storage module (40) to the chip processing module (20) for processing, and to push the microfluidic chip (80) after being processed by the chip processing module (20) to the chip recycling module (50) for recycling; The sample loading arm module (30) is located on top of the sample reagent chamber module (10) and the chip processing module (20), and is used to add the sample or reagent in the sample reagent chamber module (10) into the microfluidic chip (80) in the chip processing module (20); The chip processing module (20) includes a chip reheating chamber assembly (201), a chip positioning centrifugation assembly (202), a chip centrifugation assembly (203), a chip reaction assembly (204), and an imaging detection assembly (205) arranged sequentially along the chip transfer direction. The sample loading arm module (30) is located between the sample reagent compartment module (10) and the chip-positionable centrifugation component (202); The sample reagent compartment module (10) includes: A reagent rotary mixing mechanism (101) for loading one or more reagents to be mixed. A reagent loading mechanism (102) for loading one or more reagents that do not require mixing. Sample rack mechanism (103) for loading multiple sampling tubes; The first Y-axis linear drive mechanism (104) is directly or indirectly mounted on the base plate (602); the reagent rotation mixing mechanism (101) and the reagent loading mechanism (102) are respectively connected to the first Y-axis linear drive mechanism (104), and the first Y-axis linear drive mechanism (104) is configured to drive the reagent rotation mixing mechanism (101) and the reagent loading mechanism (102) to reciprocate synchronously along the Y-axis direction; And a sample rack pusher mechanism (105) is directly or indirectly installed on the base plate (602) and connected to the sample rack mechanism (103). The sample rack pusher mechanism (105) is configured to drive the sample rack mechanism (103) to reciprocate along the Y-axis. The sample loading arm module (30) includes: First injection needle assembly (301); Second injection needle assembly (302); The second mounting bracket (306) is fixedly mounted on the base plate (602); The first X-axis linear motion mechanism (303) and the second X-axis linear motion mechanism (304) are respectively mounted on the second mounting bracket (306) at intervals. The fourth Z-axis linear motion mechanism (309) is mounted on the first X-axis linear motion mechanism (303) and connected to the first injection needle assembly (301); The fifth Z-axis linear motion mechanism (308) is mounted on the second X-axis linear motion mechanism (304) and connected to the second dispensing needle assembly (302); The first injection needle cleaning position (3051) and the second injection needle cleaning position (3052) are located on the movement path of the first injection needle assembly (301) and the second injection needle cleaning position (3052) are located on the movement path of the second injection needle assembly (302).

2. The fully automated blood typing analyzer based on a centrifugal microfluidic chip according to claim 1, characterized in that, The chip positioning centrifugation assembly (202), the chip centrifugation assembly (203), and the imaging detection assembly (205) each include at least a rotary motion mechanism, a rotating body fixing seat, and a lifting chip tray. The rotary motion mechanism is disposed on the base plate (602), and the rotating body fixing seat is connected to the output shaft of the rotary motion mechanism. The lifting chip tray can be vertically and vertically disposed on the base plate (602) between the chip loading position and the chip mounting position along the Z-axis. When the lifting chip tray is in the chip loading position, the microfluidic chip (80) it carries is located in the chip pusher mold. On the moving path of block (70), at this time, the chip pusher module (70) can push the microfluidic chip (80) currently carried by the lifting chip tray to the next station or push the microfluidic chip (80) of the previous station into the current lifting chip tray. The pushed-in microfluidic chip (80) is supported on the lifting chip tray and can move with the lifting chip tray. When the lifting chip tray descends to the chip loading position, the microfluidic chip (80) separates from the lifting chip tray and is circumferentially limited and sleeved on the outside of the rotating body fixing seat so as to rotate with the rotating body fixing seat.

3. The fully automated blood typing analyzer based on a centrifugal microfluidic chip according to claim 2, characterized in that, The chip reheating chamber assembly (201), the chip positioning centrifugation assembly (202), the chip centrifugation assembly (203), and the chip reaction assembly (204) are arranged sequentially along the Y-axis; the chip reaction assembly (204), the imaging detection assembly (205), and the chip recovery chamber module (50) are arranged sequentially along the X-axis. The chip pusher module (70) includes a first X-axis chip pusher assembly (703), a second X-axis chip pusher assembly (702), and a Y-axis chip pusher assembly (701) that are respectively directly or indirectly mounted on the base plate (602). The first X-axis chip pusher assembly (703) is provided with a first X-axis chip pusher. The first X-axis chip pusher moves in a straight line along the X-axis direction to push the new microfluidic chip (80) from the new chip storage module (40) to the chip warming chamber assembly (201). The Y-axis chip pusher assembly (701) is equipped with a Y-axis chip pusher, which makes a linear reciprocating motion along the Y-axis direction to push out the microfluidic chip (80) in the chip warming chamber assembly (201) and pass through the chip positioning centrifuge assembly (202), the chip centrifuge assembly (203) and the chip reaction assembly (204) in sequence. The second X-axis chip pusher assembly (702) is provided with a second X-axis chip pusher. The second X-axis chip pusher moves in a straight line along the X-axis direction to push out the microfluidic chip (80) in the chip reaction assembly (204) and pass through the imaging detection assembly (205) and the chip recycling bin module (50) in sequence.

4. The fully automated blood typing analyzer based on a centrifugal microfluidic chip according to claim 3, characterized in that, The chip warming chamber assembly (201) includes: The first multi-layer chip storage mechanism (220) has multiple chip accommodating parts arranged along the Z-axis direction; each chip accommodating part is used to accommodate a microfluidic chip (80); the chip accommodating part has openings on both sides along the Y-axis direction corresponding to the Y-axis chip pusher of the Y-axis chip pusher assembly (701) and the chip positioning centrifugal assembly (202), and has an opening on one side along the X-axis direction corresponding to the first X-axis chip pusher of the first X-axis chip pusher assembly (703); And a first Z-axis linear drive mechanism (217) is directly or indirectly mounted on the base plate (602) and connected to the first multi-layer chip storage mechanism (220) to drive the first multi-layer chip storage mechanism (220) to move linearly back and forth along the Z-axis direction; When the chip receiving portion of the chip to be received moves to the moving path of the first X-axis chip pusher assembly (703), the new microfluidic chip (80) is pushed from the new chip storage module (40) into the chip receiving portion of the chip to be received by the first X-axis chip pusher of the first X-axis chip pusher assembly (703); when the chip receiving portion of the chip to be transferred moves to the moving path of the Y-axis chip pusher assembly (701), the warmed microfluidic chip (80) is pushed into the chip positioning centrifuge assembly (202) by the Y-axis chip pusher of the Y-axis chip pusher assembly (701).

5. The fully automated blood typing analyzer based on a centrifugal microfluidic chip according to claim 3, characterized in that, The chip-positionable centrifugal assembly (202) also includes a QR code scanning mechanism (213), and the rotational motion mechanism in the chip-positionable centrifugal assembly (202) is a first rotational motion mechanism (214); the QR code scanning mechanism (213) and the first rotational motion mechanism (214) work together to complete the scanning of the QR code on the surface of the microfluidic chip (80).

6. The fully automated blood typing analyzer based on a centrifugal microfluidic chip according to claim 3, characterized in that, The chip reaction assembly (204) includes a second multi-layer chip storage mechanism (221) and a third Z-axis linear drive mechanism (218). The second multi-layer chip storage mechanism (221) is used to receive and hold the microfluidic chip (80) from the chip centrifugation assembly (203). The third Z-axis linear drive mechanism (218) is configured to drive the second multi-layer chip storage mechanism (221) to move linearly back and forth along the Z-axis direction, and cooperate with the Y-axis chip pusher assembly (701) to transfer the held microfluidic chip (80) to the imaging detection assembly (205).

7. The fully automated blood typing analyzer based on a centrifugal microfluidic chip according to claim 3, characterized in that, The imaging detection component (205) also includes an imaging mechanism (209), and the rotational motion mechanism in the imaging detection component (205) is a rotational drive mechanism (216); the rotational drive mechanism (216) can drive the microfluidic chip (80) loaded on it to rotate at any position, and cooperate with the imaging mechanism (209) to complete the imaging detection of each reaction hole in the microfluidic chip (80); after the detection is completed, the microfluidic chip (80) is transferred to the chip recycling bin module (50) under the drive of the second X-axis chip pusher of the second X-axis chip pusher component (702).

8. The fully automated blood typing analyzer based on a centrifugal microfluidic chip according to claim 3, characterized in that, The new chip storage module (40) includes one or more storage units arranged side by side along the X-axis. Each storage unit includes a cabinet (406), a lifting chip compartment (401), and a sixth Z-axis linear motion mechanism corresponding to each lifting chip compartment (401). The lifting chip compartment (401) has a accommodating space for storing multiple vertically stacked microfluidic chips (80). When the lifting chip compartment (401) is in the lowered position, the lifting chip compartment (401) and the cabinet (406) enclose the accommodating space, and the tops of all the lifting chip compartments (401) and the cabinet (406) together form a chip delivery channel. Each of the sixth Z-axis linear motion mechanisms is installed on the corresponding cabinet (406), and each lifting chip compartment (401) is connected to the corresponding sixth Z-axis linear motion mechanism. Each of the sixth Z-axis linear motion mechanisms is configured to drive the corresponding lifting chip compartment (401) to perform linear lifting motion along the Z-axis. The first X-axis chip pusher assembly (703) is disposed on one side of the chip transport channel; the sixth Z-axis linear motion mechanism works together with the first X-axis chip pusher assembly (703) to transfer the microfluidic chip (80) of the lifting chip compartment (401) along the chip transport channel to the chip processing module (20).

9. The fully automated blood typing analyzer based on a centrifugal microfluidic chip according to claim 3, characterized in that, The chip recycling bin module (50) includes: The outer frame of the recycling bin (508) has a pull-out space (509) inside. A chip collection device for collecting used microfluidic chips (80) is provided inside the outer frame (508) of the recycling bin, which can be pulled out of the pull-out space (509) and retracted from the pull-out space (509); a chip drop outlet (510) is provided at the top of the pull-out space (509) corresponding to the position of the chip collection device in the retracted position. A pair of guide rails (501) are disposed above the chip drop port (510). The pair of guide rails (501) have a first state in which they are close to each other to carry the used microfluidic chip (80) from the chip processing module (20) and a second state in which they are far apart and spread out so that the used microfluidic chip (80) carried by them falls through the chip drop port (510) into the chip collection device. And a guide rail opening drive mechanism (502) for driving the pair of guide rails (501) to move closer to each other and further away from each other, the guide rail opening drive mechanism (502) is mounted on the base plate (602) through the outer frame (508) of the recycling bin; the pair of guide rails (501) are connected to the guide rail opening drive mechanism (502).

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