Reagent assembly and analyzer

Abstract

The reagent assembly comprises a reagent bottle and a suction pipe assembly, a first limiting piece and a second limiting piece of the suction pipe assembly are arranged at the first end of a reagent suction pipe, and the first limiting piece and the second limiting piece are arranged in the length direction of the reagent suction pipe. The projection of the second through hole of the second limiting piece on the first limiting piece is not overlapped with the first through hole of the first limiting piece. When a reagent in the reagent bottle is sucked out through the reagent suction pipe, external gas can enter the reagent bottle through the second through hole and the first through hole, and the projection of the second through hole on the first limiting piece is not overlapped with the first through hole; the external gas cannot directly pass through the second through hole and the first through hole to form a smooth flow path to enter the reagent bottle, so that the speed of the gas entering the reagent bottle is slowed down, the amount of the gas entering the reagent bottle is reduced, the condition that a reagent in the reagent bottle goes bad due to reaction with the gas is reduced, and the utilization rate of the reagent in the reagent bottle is increased; the reagent cost is reduced.

Description

Technical Field

[0001] This application relates to the field of reagent aspiration and dispensing technology, and in particular to a reagent assembly and analyzer. Background Technology

[0002] During sample analysis, some samples require staining or other procedures to detect cells. Therefore, analyzers typically include a reagent module to provide reagents for auxiliary testing. However, the reagent pipette used to draw reagents from the vial and the soft cap on the vial's opening are highly permeable. Once opened, the vial cannot be completely sealed, allowing the reagent to absorb moisture and oxygen from the air. Over time, this leads to a chemical reaction and deterioration of the reagent, rendering it ineffective. Users must then replace the vial, increasing the analyzer's reagent costs. Summary of the Invention

[0003] To address the aforementioned technical problems, this application provides a reagent assembly. The reagent assembly includes a reagent bottle and a pipette assembly, with one end of the pipette assembly disposed inside the reagent bottle to draw reagent from the bottle.

[0004] The pipette assembly includes a reagent pipette, a first limiting member, a second limiting member, and a sealing cap. The first limiting member and the second limiting member are spaced apart at the first end of the reagent pipette along the length direction of the reagent pipette. When the second end of the reagent pipette is located inside the reagent bottle, the first limiting member abuts against the mouth of the reagent bottle.

[0005] The sealing cap is embedded between the first limiting member and the second limiting member, and abuts against the surface of the first limiting member near the second limiting member and the surface of the second limiting member near the first limiting member, respectively.

[0006] The first limiting member is provided with a first through hole, and the second limiting member is provided with a second through hole. Along the length direction of the reagent pipette, the projection of the second through hole on the first limiting member does not overlap with the first through hole.

[0007] The first limiting member, the second limiting member, and the sealing cap form a gas flow cavity on the side of the reagent pipette, and the reagent bottle circulates with the external environment through the first through hole, the gas flow cavity, and the second through hole.

[0008] The radial area of ​​the first through hole is less than 1 square millimeter.

[0009] When the second end of the reagent pipette is inside the reagent bottle, the first limiting member abuts against the mouth of the reagent bottle, and the sealing cap is placed on the mouth of the reagent bottle; and during the process of the reagent pipette drawing reagent from the reagent bottle, external gas enters the gas flow chamber through the second through hole, and enters the reagent bottle from the gas flow chamber through the first through hole.

[0010] The inner wall of the sealing cap includes a protrusion that abuts against the mouth of the reagent bottle. Under the elastic squeezing force of the sealing cap, the protrusion is used to seal the mouth of the reagent bottle.

[0011] The reagent bottle has a sealing film at its opening, and the pipette assembly has a puncture part. When the reagent pipette is inserted into the reagent bottle, the puncture part is used to tear the sealing film to form an opening, through which the second end of the reagent pipette enters the reagent bottle.

[0012] When the second end of the reagent pipette is located inside the reagent bottle, the first limiting member is in contact with the sealing film, and along the length direction of the reagent pipette, the projection of the opening on the first limiting member at least partially overlaps with the first through hole.

[0013] The reagent assembly further includes a placement bracket, which includes a reagent bottle placement bracket and a pipette placement bracket. The reagent bottle placement bracket is used to place and fix the reagent bottle. The pipette placement bracket is detachably mounted on the reagent bottle placement bracket and is used to place the pipette assembly when the pipette assembly is detached from the reagent bottle.

[0014] The reagent bottle has a groove at the bottom, which corresponds to the opening of the reagent bottle, and the second end of the reagent pipette is located in the groove.

[0015] To address the aforementioned technical problems, this application also provides an analyzer, including a reagent component and a detection component as described above. The detection component and the reagent component are spaced apart. During the sample detection process of the detection component, the reagent component provides the detection component with reagents from a reagent bottle to assist the detection component in performing sample detection.

[0016] The analyzer housing is provided with a replacement window, which is correspondingly set with the reagent assembly. After the reagent in the reagent bottle is completely drawn out, the reagent bottle is replaced through the replacement window.

[0017] The beneficial effects of this application are as follows: Unlike the prior art, the reagent assembly of this application includes a reagent bottle and a pipette assembly. One end of the pipette assembly is disposed inside the reagent bottle to draw reagent from the reagent bottle. The pipette assembly includes a reagent pipette, a first limiting member, a second limiting member, and a sealing cap. The first and second limiting members are spaced apart along the length of the reagent pipette at the first end of the reagent pipette. When the second end of the reagent pipette is inside the reagent bottle, the first limiting member abuts against the bottle opening to isolate the bottle opening from the outside environment and prevent excessive external gas from entering the reagent bottle. The sealing cap is embedded between the first limiting member and the second limiting member, and abuts against the surface of the first limiting member near the second limiting member and the surface of the second limiting member near the first limiting member, respectively. The first limiting member has a first through hole, and the second limiting member has a second through hole. The first limiting member, the second limiting member, and the sealing cap form a gas flow cavity on the side of the reagent pipette. Gas can enter the gas flow cavity through the second through hole and enter the reagent bottle through the first through hole. Furthermore, along the length of the reagent pipette, the projection of the second through hole on the first limiting member does not overlap with the first through hole. When the reagent in the reagent bottle is drawn out through the reagent pipette, external gas will enter the reagent bottle through the second through hole and the first through hole. However, since the projection of the second through hole on the first limiting member does not overlap with the first through hole, the external gas cannot directly enter the reagent bottle through the second through hole and the first through hole to form a smooth flow path. This slows down the rate at which gas enters the reagent bottle, reduces the amount of gas entering the reagent bottle, and reduces the possibility of the reagent in the reagent bottle reacting with the gas and deteriorating. This improves the utilization rate of the reagent in the reagent bottle and reduces the reagent cost. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] in:

[0020] Figure 1 This is a schematic diagram of the structure of the reagent component of the first embodiment of this application;

[0021] Figure 2 This is a schematic diagram of the structure of the first embodiment of the straw assembly of this application;

[0022] Figure 3 This is a schematic diagram of the structure of the first embodiment of the sealing cap of this application;

[0023] Figure 4 yes Figure 1 A schematic diagram of the cross-sectional structure of the reagent component along section line I-I';

[0024] Figure 5 yes Figure 3 A schematic diagram of the cross-sectional structure of the central sealing cover along section line II-II';

[0025] Figure 6 This is a schematic diagram of the structure of the reagent component of the second embodiment of this application;

[0026] Figure 7 This is a schematic diagram of the structure of the first embodiment of the analyzer of this application.

[0027] Reference numerals: Analyzer A; Reagent assembly 1; Reagent bottle 11; Groove 111; Pipette assembly 12; Reagent pipette 121; First limiting member 122; First through hole 1221; Second limiting member 123; Second through hole 1231; Sealing cap 124; Gas flow chamber 1241; Protrusion 1242; Placement bracket 13; Reagent bottle placement bracket 131; Elastic part 1311; Pipette placement bracket 132; Replacement window 2. Detailed Implementation

[0028] The embodiments of this application will now be described in detail with reference to the accompanying drawings.

[0029] In the following description, specific details such as particular system architectures, interfaces, and technologies are presented for illustrative purposes rather than for limiting purposes, in order to provide a thorough understanding of this application.

[0030] In this application, the reference to "embodiment" means that a specific feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0031] In this application, the term "and / or" is merely a description of the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent three cases: A alone, A and B simultaneously, and B alone. Additionally, the character " / " generally indicates that the preceding and following related objects are in an "or" relationship. Furthermore, "many" in this application means two or more. Moreover, the term "at least one" in this application means any combination of at least two of any one or more of a plurality of objects. For example, including at least one of A, B, and C can mean including any one or more elements selected from the set consisting of A, B, and C. Furthermore, the terms "first," "second," and "third" in this application are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features.

[0032] Please see Figure 1 , Figure 1 This is a schematic diagram of the structure of the first embodiment of the reagent assembly of this application. The reagent assembly 1 provided by this application includes a reagent bottle 11 and a pipette assembly 12. The reagent bottle 11 is used to hold reagents, and one end of the pipette assembly 12 is disposed inside the reagent bottle 11 to draw the reagents from the reagent bottle 11.

[0033] Optionally, please refer to Figures 2-4 , Figure 2 This is a structural schematic diagram of the first embodiment of the straw assembly of this application. Figure 3 This is a structural schematic diagram of the first embodiment of the sealing element of this application. Figure 4 yes Figure 1 A schematic diagram of the cross-sectional structure of the reagent assembly along section line I-I'. The pipette assembly 12 provided in this application embodiment includes a reagent pipette 121, a first limiting member 122, a second limiting member 123, and a sealing cap 124.

[0034] Specifically, such as Figure 2 As shown, the first limiting member 122 and the second limiting member 123 are spaced apart along the length of the reagent pipette 121 at the first end of the reagent pipette 121. When the second end of the reagent pipette 121 is inside the reagent bottle 11, as... Figure 4 As shown, the first limiting member 122 abuts against the mouth of the reagent bottle 11. Furthermore, during the process of the pipette assembly 12 being placed inside the reagent bottle 11 to draw the reagent, the first limiting member 122 covers the mouth of the reagent bottle 11 to prevent excessive external gas from entering the reagent bottle 11 and reacting with the reagent inside the reagent bottle 11.

[0035] The sealing cap 124 is embedded between the first limiting member 122 and the second limiting member 123, and respectively abuts against the surface of the first limiting member 122 near the second limiting member 123 and the surface of the second limiting member 123 near the first limiting member 122.

[0036] In one embodiment, if the reagent bottle 11 is completely sealed during the process of the pipette assembly 12 drawing reagent from the reagent bottle 11, the air pressure inside the reagent bottle 11 will decrease due to the reagent being drawn out, hindering subsequent reagent drawing. Therefore, it is necessary to provide a through hole to allow a small amount of gas to enter the reagent bottle 11 in order to maintain the stability of the air pressure inside the reagent bottle 11 and ensure the smoothness of reagent drawing.

[0037] Specifically, in this embodiment, the first limiting member 122 is provided with a first through hole 1221, and the second limiting member 123 is provided with a second through hole 1231. Furthermore, the first limiting member 122, the second limiting member 123, and the sealing cap 124 form a gas flow cavity 1241 on the side of the reagent pipette 121, and the gas flow cavity 1241 circulates with the external environment through the second through hole 1231 and the first through hole 1221.

[0038] When the second end of the reagent pipette 121 is inside the reagent bottle 11, the first limiting member 122 abuts against the mouth of the reagent bottle 11, and the sealing cap 124 is placed on the mouth of the reagent bottle 11. The sealing cap 124 seals the side wall of the mouth of the bottle through its own elastic squeezing force, preventing external gas from entering the reagent bottle 11 through the side wall of the mouth of the bottle.

[0039] During the process of the reagent pipette 121 drawing reagent from the reagent bottle 11, as the reagent is drawn out, the air pressure inside the reagent bottle 11 decreases. At this time, due to the air pressure difference between the inside of the reagent bottle 11 and the external environment, external gas enters the gas flow chamber 1241 through the second through hole 1231 and enters the reagent bottle 11 from the gas flow chamber 1241 through the first through hole 1221, maintaining the air pressure balance inside the reagent bottle 11 and ensuring the smoothness of the pipette assembly 12 in drawing reagent from the reagent bottle 11.

[0040] In one embodiment, the sealing cap 124 can be made of an elastic material such as a rubber soft cap, thereby ensuring the sealing of the sidewall of the gas flow cavity 1241 under the action of its own elastic extrusion force.

[0041] Furthermore, along the length of the reagent pipette 121, the projection of the second through hole 1231 on the first limiting member 122 does not overlap with the first through hole 1221. Therefore, when gas enters the gas flow chamber 1241 through the second through hole 1231, the gas accumulates in the gas flow chamber 1241 before entering the reagent bottle 11 through the first through hole 1221. This slows down the rate at which gas enters the reagent bottle 11, reduces the contact between the gas and the reagent in the reagent bottle 11, and prevents excessive gas from entering the reagent bottle 11 and causing the reagent in the reagent bottle 11 to deteriorate. This further improves the sealing performance of the pipette assembly 12 to the reagent bottle 11.

[0042] In this related technology, along the length of the reagent pipette 121, the projection of the second through hole 1231 on the first limiting member 122 overlaps with the first through hole 1221. That is, the second through hole 1231 is directly opposite the first through hole 1221. Gas forms a smooth gas path at the first through hole 1221 and the second through hole 1231. Therefore, external gas, after passing through the second through hole 1231, will directly enter the reagent bottle 11 through the first through hole 1221. Compared to the case where the projections of the first through hole 1221 and the second through hole 1231 do not overlap, the gas flow rate is higher when the first through hole 1221 is directly opposite the second through hole 1231. Therefore, in this embodiment, the first through hole 1221 and the second through hole 1231 are not overlapped on the same projection plane to slow down the rate at which gas enters the reagent bottle 11, reduce the amount of gas entering the reagent bottle 11, reduce the occurrence of gas reacting with the reagent in the reagent bottle 11 and causing the reagent to deteriorate, ensure the safety of the reagent in the reagent bottle 11, and improve the utilization rate of the reagent in the reagent component 1.

[0043] Meanwhile, since the projection of the second through hole 1231 on the first limiting member 122 does not overlap with the first through hole 1221 along the length direction of the reagent pipette 121, substances such as dust carried in the external gas will not reach the first through hole 1221 through the second through hole 1231, causing blockage of the first through hole 1221 and affecting the stability of reagent aspiration of the pipette assembly 12.

[0044] In one embodiment, such as Figure 2 As shown, on the surface of the first limiting member 122, the angle formed by the radial line connecting the first through hole 1221 and the reagent pipette 121, and the radial line connecting the projection of the second through hole 1231 on the first limiting member 122 and the reagent pipette 121 (hereinafter referred to as the intersection angle between the first through hole 1221 and the second through hole 1231) can be 180°. In other embodiments, the intersection angle between the first through hole 1221 and the second through hole 1231 can also be other non-zero degrees, and this application does not limit it.

[0045] Optionally, the radial area of ​​the first through hole 1221 is less than 1 square millimeter.

[0046] As mentioned above, the gas entering the gas flow chamber 1241 through the second through hole 1231 carries dust and other substances. In order to prevent dust and other substances from entering the reagent bottle 11 and affecting the reagent in the reagent bottle 11, this application embodiment proposes that the radial area of ​​the first through hole 1221 is less than 1 square millimeter, so as to filter the gas entering the reagent bottle 11, avoid other substances carried in the gas from affecting the reagent in the reagent bottle 11, and improve the safety of the reagent in the reagent bottle 11.

[0047] Optionally, such as Figure 5 As shown, Figure 5 yes Figure 3 A cross-sectional structural diagram of the sealing element along section line II-II'. The inner wall of the sealing cap 124 includes a protrusion 1242. Please refer to... Figure 4 When the sealing cap 124 is placed on the mouth of the reagent bottle 11, the protrusion 1242 abuts against the mouth of the reagent bottle 11. Under the elastic squeezing force of the sealing cap 124, the protrusion 1242 can seal the mouth of the reagent bottle 11, preventing external gas from entering the reagent bottle 11 through the side wall of the mouth of the reagent bottle 11, ensuring the sealing of the reagent bottle 11 during the reagent aspiration process, and ensuring the safety of the reagent in the reagent bottle 11.

[0048] In one embodiment, the absorbance value of the staining reagent is measured to determine whether the reagent has become ineffective. Absorbance is the reagent's ability to absorb light. When the reagent reacts with moisture or oxygen in the air and deteriorates, its absorbance value will change accordingly. Specifically, if the change in the absorbance value of the reagent during use exceeds ±10% of the absorbance label value (the absorbance value when the reagent is newly opened), the reagent is considered to have deteriorated and become ineffective.

[0049] In this embodiment, Dimai D-80FP reagent was used for the experiment;

[0050] Experiment 1:

[0051] Take three bottles of D-80FP reagent of the same batch number and specification, and label them as D-80FP reagent No. 1, D-80FP reagent No. 2, and D-80FP reagent No. 3. Among them, D-80FP reagent No. 1 is the control group. The absorbance value of D-80FP reagent No. 1 when it is freshly opened is 0.589 (since D-80FP reagent No. 1, D-80FP reagent No. 2, and D-80FP reagent No. 3 are D-80FP reagents of the same batch number, it can be considered that the absorbance label value of D-80FP reagent No. 2 and D-80FP reagent No. 3 is the experimental group. After opening D-80FP reagent No. 2, it is connected to the relevant pipette assembly (the projection of the second through hole on the first limiting member overlaps with the first through hole); after opening D-80FP reagent No. 3, it is connected to the pipette assembly 12 of this application. After treating reagents No. 2 and No. 3, and placing them in the same environment, the absorbance value of reagent No. 2 was measured to be -17.01% after 90 days, while the absorbance value of reagent No. 3 was -7.30%. The experimental results show that the absorbance value change of reagent No. 3 connected to the pipette assembly 12 of this application is within the standard range, and the absorbance value change is significantly less than that of reagent No. 2 connected to the relevant pipette assembly. This indicates that the pipette assembly 12 of this application has a significant improvement and optimization effect compared to related technologies. The sealing effect of the pipette assembly 12 on the reagent bottle 11 is significantly stronger than that of related pipette assemblies, thus realizing the practicality of the reagent assembly 1 of this application.

[0052] Experiment 2:

[0053] Five bottles of D-80FP reagent from the same batch, each with a specification of 6 mL, were taken and labeled as D-80FP Reagent No. 4, D-80FP Reagent No. 5, D-80FP Reagent No. 6, D-80FP Reagent No. 7, and D-80FP Reagent No. 8. D-80FP Reagent No. 4 served as the control group. The absorbance value of D-80FP Reagent No. 4 upon opening was 0.589 (since D-80FP Reagent No. 4, 5, 6, 7, and 8 are from the same batch, their absorbance values ​​can be considered to be 0.589).

[0054] Reagents 5, 6, 7, and 8 of D-80FP constitute the experimental group;

[0055] Among them, reagent No. 5 D-80FP can be treated by opening and adding water and then tightening the reagent bottle cap (without through holes). Reagent No. 6 D-80FP can be treated by opening and adding water and then connecting the pipette assembly 12 of this application to the mouth of the reagent bottle. The water addition treatment is to add 0.6g of purified water to simulate 46 days after opening of D-80FP reagent. In this experiment, the treated reagent No. 5 D-80FP and the treated reagent No. 6 D-80FP were placed in the same environment. After 49 days, the weight gain of reagent No. 5 D-80FP was 0.0232g, while the weight gain of reagent No. 6 D-80FP was 0.1234g. This indicates that some gas in reagent No. 5 D-80FP still enters the reagent bottle through the reagent bottle cap. Since the pipette assembly 12 is provided with the first through hole 1221 and the second through hole 1231, the weight gain of reagent No. 6 D-80FP is greater than that of reagent No. 5 D-80FP.

[0056] At this time, the absorbance value of reagent No. 5 (D-80FP) is 0.534, and the change in absorbance value (compared to the absorbance label value) is -9.34%; the absorbance value of reagent No. 6 (D-80FP) is 0.546, and the change in absorbance value is -7.30%. That is, after 46 days + 49 days = 95 days, the change in absorbance value of reagent No. 5 (D-80FP) is -9.34%, and the change in absorbance value of reagent No. 6 (D-80FP) is -7.30%. The test results meet the requirement that the change in absorbance value of the reagents is within ±10% of the absorbance label value. This means that the reagent component 1 provided in this application has a good sealing effect on the opened D-80FP reagent. Compared with re-capping the bottle after opening, connecting it to the pipette component 12 after opening can slow down the deterioration of the D-80FP reagent and meet the performance stability requirements for use after opening. This demonstrates the practicality of the pipette component 12 provided in this application embodiment.

[0057] Furthermore, both reagents No. 7 and No. 8 were opened, water was added, and then they were connected to the pipette assembly 12 of this application. 100 μL of reagent was taken from reagent No. 7 and 200 μL from reagent No. 8 daily. After 19 days, a total of 1.9 mL of reagent was taken from reagent No. 7 and a total of 3.8 mL of reagent was taken from reagent No. 8. At this point, reagent No. 7... The absorbance value of reagent D-80FP is 0.552, and the change in absorbance value (compared to the labeled absorbance value) is -6.28%; the absorbance value of reagent D-80FP #8 is 0.531, and the change in absorbance value is -9.85%. That is, after 46 days + 19 days = 65 days, the absorbance value change of reagent D-80FP #7 is -6.28%, and the absorbance value change of reagent D-80FP #8 is -9.85%. The test results meet the requirement that the change in absorbance value of the reagents is within ±10% of the labeled absorbance value, which means that the pipette assembly 12 provided in this application still has a good sealing effect on the D-80FP reagent used in the instrument.

[0058] Among them, after 46 days + 19 days = 65 days, the change in absorbance value of reagent No. 7 D-80FP is less than that of reagent No. 8 D-80FP. This is because reagent No. 8 D-80FP absorbs a larger amount of reagent each day, resulting in more gas entering the reagent bottle compared to reagent No. 7 D-80FP. However, the change in absorbance value of reagent No. 8 D-80FP after 65 days is still within the standard range, thus realizing the practicality of reagent component 1 provided in this application.

[0059] Experiment 3:

[0060] Take four bottles of D-80FP reagent with the same batch number as Experiment 2 and a specification of 12mL, and label them as D-80PF reagent No. 9, D-80PF reagent No. 10, D-80PF reagent No. 11 and D-80PF reagent No. 12. D-80PF reagent No. 9, D-80PF reagent No. 10, D-80PF reagent No. 11 and D-80PF reagent No. 12 are the experimental group, and D-80FP reagent No. 4 is the control group. Among them, reagent No. 9 (D-80FP) can be treated by opening, adding water, and then tightening the bottle cap. Reagent No. 10 (D-80FP) can be treated by opening, adding water, and then connecting the pipette assembly 12 of this application to the bottle mouth. The water addition treatment involves adding 0.6g of purified water to simulate 46 days after opening the D-80FP reagent. In this experiment, the treated reagents No. 9 and No. 10 were placed in the same environment as reagents No. 5 and No. 6. After 49 days, the weight gain of reagent No. 9 was 0.0157g, while the weight gain of reagent No. 10 was 0.138g. 3g. At this time, the absorbance value of reagent No. 9 D-80FP is 0.561, and the change in absorbance value is -4.75%; the absorbance value of reagent No. 10 D-80FP is 0.555, and the change in absorbance value is -5.77%. That is, after 46 days + 49 days = 95 days, the change in absorbance value of reagent No. 9 D-80FP is -4.75%, and the change in absorbance value of reagent No. 10 D-80FP is -5.77%. The test results meet the requirement that the change in absorbance value of the reagent is within ±10% of the absorbance label value. This means that the reagent component 1 provided in this application also has a good sealing effect on D-80FP reagents that have been opened and are of large size.

[0061] Furthermore, both reagents No. 11 and No. 12 were opened, water was added, and then they were connected to the pipette assembly 12 of this application. 200 μL of reagent was taken daily from reagent No. 11 and 400 μL from reagent No. 12. After 19 days, a total of 3.8 mL of reagent was taken from reagent No. 11 and a total of 7.6 mL from reagent No. 12. The absorbance of reagent 11 (D-80FP) was 0.549, with a change of -6.79%; the absorbance of reagent 12 (D-80FP) was 0.541, with a change of -8.15%. That is, after 46 days + 19 days = 65 days, the absorbance change for reagent 11 (D-80FP) was -6.79%, and for reagent 12 (D-80FP) was -8.15%. The test results meet the requirement that the absorbance change of the reagents is within ±10% of the labeled absorbance value. This indicates that reagent component 1 provided in this application still has a good sealing effect on the D-80FP reagent used in the instrument, realizing the practicality of reagent component 1 provided in this application.

[0062] Experiments 2 and 3 were conducted under the same conditions. Data from experiments with reagents 5, 6, 9, and 10 of D-80FP showed that the amount of water absorbed (weight gain) after 96 days was not significantly different between the 6mL and 12mL D-80FP reagents. However, the absorbance change of the 12mL D-80FP reagent was less than that of the 6mL reagent, and the risk of the 12mL D-80FP reagent exceeding the standard was lower than that of the 6mL reagent. In other words, for reagent bottles 11 after opening and standing, the pipette assembly 12 of this application provides better sealing performance for larger reagent bottles 11.

[0063] Furthermore, experimental data from reagents 7, 8, 11, and 12 of the D-80FP series also show that the pipette assembly 12 of this application provides better sealing performance for larger reagent bottles 11 used in the instrument.

[0064] In summary, in the reagent assembly 1 provided in this application, the pipette assembly 12 has a better sealing effect on the reagent bottle 11 than the related pipette assemblies. The sealing effect of the pipette assembly 12 on the reagent bottle 11 is stronger than that of the related pipette assemblies.

[0065] Optionally, the reagent bottle 11 is provided with a sealing film (not shown) at the bottle opening, so that when the pipette assembly 12 is not in the reagent bottle 11, the sealing film seals the reagent bottle 11, preventing the reagent in the reagent bottle 11 from coming into contact with external gas and ensuring the safety of the reagent in the reagent bottle 11.

[0066] The pipette assembly 12 has a puncture part. Specifically, the puncture part can be provided at the sealing cap 124. Before the second end of the reagent pipette 121 enters the reagent bottle 11, the puncture part can first tear an opening at the sealing film, and then the second end of the reagent pipette 121 enters the reagent bottle 11 through the opening. At this time, the first limiting member 122 abuts against the bottle mouth of the reagent bottle 11, and the sealing cap 124 is placed on the bottle mouth of the reagent bottle 11 to further ensure the sealing of the reagent bottle 11.

[0067] In another embodiment, the puncture portion can also be provided at the second end of the reagent pipette 121. For example, the second end of the reagent pipette 121 is a conical surface. Then, before the second end of the pipette assembly 12 enters the reagent bottle 11, the second end of the reagent pipette 121 can puncture the sealing film to form an opening at the sealing film. After the opening is formed, the reagent pipette 121 directly enters the reagent bottle 11 through the opening.

[0068] In other embodiments, the puncture portion may also be provided at other parts of the pipette assembly 12, and when the puncture portion is provided at other parts of the pipette assembly 12, the second end of the reagent pipette 121 may be a circular plane, which is not limited in this application.

[0069] When the second end of the reagent pipette 121 is inside the reagent bottle 11, the first limiting member 122 and the sealing film will come into contact. Along the length direction of the reagent pipette 121, the projection of the opening on the first limiting member 122 at least partially overlaps with the first through hole 1221, so that gas can enter the reagent bottle 11 through the first through hole 1221 and the opening.

[0070] In one embodiment, if the projection of the opening on the first limiting member 122 does not overlap with the first through hole 1221, that is, when the first limiting member 122 contacts the sealing film, the sealing film will block the first through hole 1221, and gas will be unable to enter the reagent bottle 11 through the first through hole 1221, affecting the smoothness of the reagent pipette 121 in drawing reagent from the reagent bottle 11. Therefore, this application proposes that when the second end of the reagent pipette 121 is located inside the reagent bottle 11, the first limiting member 122 and the sealing film will contact each other, and along the length direction of the reagent pipette 121, the projection of the opening on the first limiting member 122 overlaps with the first through hole 1221 at least partially, ensuring that gas can enter the reagent bottle 11 through the first through hole 1221, thus ensuring the efficiency of reagent absorption in the reagent assembly 1.

[0071] Optionally, such as Figure 6 As shown, Figure 6 This is a schematic diagram of the structure of the second embodiment of the reagent assembly of this application. The placement bracket 13 provided in this embodiment includes a reagent bottle placement bracket 131 and a pipette placement bracket 132.

[0072] The reagent bottle placement bracket 131 is used to place and fix the reagent bottle 11. The pipette placement bracket 132 is detachably mounted on the reagent bottle placement bracket 131. When the pipette assembly 12 is detached from the reagent bottle 11, the pipette placement bracket 132 is used to place the pipette assembly 12.

[0073] For specific details, please refer to... Figure 4 As shown, the reagent bottle placement bracket 131 includes an elastic part 1311. When the reagent bottle 11 is placed on the reagent bottle placement bracket 131, the elastic part 1311 abuts against the reagent bottle 11 due to the elastic force, thereby restricting the movement of the reagent bottle 11 and fixing the reagent bottle 11. This prevents the reagent bottle 11 from becoming unstable and shaking during the reagent aspiration process, which would cause the reagent assembly 1 to fail to aspirate the reagent normally, thus improving the stability of the reagent assembly 1.

[0074] Furthermore, the pipette placement bracket 132 can be positioned above the reagent bottle placement bracket 131 along the height direction of the placement bracket 13. Thus, when the reagent bottle 11 needs to be replaced after the reagent has been drawn out, the user can separate the pipette assembly 12 from the reagent bottle 11 and place the pipette assembly 12 directly on the pipette placement bracket 132, reducing the movement of the pipette assembly 12 and preventing contamination of the pipette assembly 12 due to improper placement.

[0075] Alternatively, please continue reading Figure 4 The bottom of the reagent bottle 11 includes a groove 111, which is correspondingly set to the mouth of the reagent bottle 11. When the second end of the reagent pipette 121 is set inside the reagent bottle 11, the second end of the reagent pipette 121 can be located in the groove 111. The reagent in the reagent bottle 11 will gather towards the groove 111 due to its own gravity. That is, it ensures that the reagent pipette 121 is in contact with the reagent before the reagent in the reagent bottle 11 is completely drawn, ensuring the smoothness of the reagent being drawn through the reagent pipette 121 and avoiding reagent waste.

[0076] In another embodiment, such as Figure 4 As shown, the bottom of the reagent bottle 11 can be an inclined surface, tilted toward the groove 111, so as to further concentrate the reagent toward the groove 111, further improve the smoothness of reagent aspiration by the pipette assembly 12, and avoid reagent waste.

[0077] In summary, the reagent assembly 1 provided in this application embodiment includes a reagent bottle 11, a pipette assembly 12, and a placement bracket 13. The placement bracket 13 is used to place the reagent bottle 11 and the pipette assembly 12. The pipette assembly 12 includes a reagent pipette 121, a first limiting member 122, a second limiting member 123, and a sealing cap 124. The sealing cap 124 is embedded between the first limiting member 122 and the second limiting member 123 to form a gas flow cavity 1241. When the second end of the reagent pipette 121 is located inside the reagent bottle 11, the sealing cap 124 is placed on the mouth of the reagent bottle 11 to seal the reagent bottle 11. Furthermore, along the length direction of the reagent pipette 121, the projection of the second through hole 1231 on the first limiting member 122 does not overlap with the first through hole 1221. Consequently, gas cannot enter the reagent bottle 11 through a direct gas circuit, slowing down the rate at which gas enters the reagent bottle 11, reducing the amount of gas entering the reagent bottle 11, and improving the safety of the reagent in the reagent bottle 11.

[0078] This application also provides an analyzer, such as Figure 7 As shown, Figure 7 This is a schematic diagram of the structure of the first embodiment of the analyzer of this application. Analyzer A includes a reagent component 1 and a detection component (not shown).

[0079] The detection component and the reagent component 1 are arranged alternately. The detection component is used to perform sample detection using sample liquid. During the sample detection process, the reagent component 1 provides the detection component with the reagent in the reagent bottle 11 to assist the detection component in performing the detection.

[0080] Specifically, the first end of the reagent pipette 121 can be connected to an air pump assembly. During sample detection by the detection assembly, the air pump assembly provides negative pressure to draw the reagent in the reagent bottle 11 through the reagent pipette 121 to the detection assembly to assist in sample detection. Simultaneously, as the air pressure inside the reagent bottle 11 decreases, external gas enters the gas flow chamber 1241 through the second through-hole 1231 and then enters the reagent bottle 11 through the first through-hole 1221 to balance the air pressure inside the reagent bottle 11. Since the projection of the second through-hole 1231 onto the first limiting member 122 does not overlap with the first through-hole 1221, the rate at which gas enters the reagent bottle 11 is slowed, thereby reducing the amount of gas entering the reagent bottle 11 and improving the safety of the reagent inside the reagent bottle 11.

[0081] Optionally, the housing of analyzer A is provided with a replacement window 2, which is set to correspond to reagent component 1. After the reagent in reagent bottle 11 is completely drawn, reagent bottle 11 is replaced through replacement window 2.

[0082] Specifically, after the reagent in reagent bottle 11 has been completely drawn out, the user can place the pipette assembly 12 on the pipette placement bracket 132 through the replacement window 2, remove the reagent bottle 11 placed on the reagent bottle placement bracket 131, place the new reagent bottle 11 on the reagent bottle placement bracket 131, and then place the pipette assembly 12 placed on the pipette placement bracket 132 back on the reagent bottle 11 so that the pipette assembly 12 can draw out the reagent in the new reagent bottle 11.

[0083] The above description is merely an embodiment of this application and does not limit the patent scope of this application. Any equivalent structural or procedural transformations made using the content of this application's specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this application.

Claims

1. A reagent assembly, characterized in that, It includes a reagent bottle and a pipette assembly, one end of which is disposed inside the reagent bottle to draw reagent from the reagent bottle through the pipette assembly; The pipette assembly includes a reagent pipette, a first limiting member, a second limiting member, and a sealing cap. The first limiting member and the second limiting member are spaced apart at the first end of the reagent pipette along the length direction of the reagent pipette. When the second end of the reagent pipette is located inside the reagent bottle, the first limiting member abuts against the mouth of the reagent bottle. The sealing cap is embedded between the first limiting member and the second limiting member, and abuts against the surface of the first limiting member near the second limiting member and the surface of the second limiting member near the first limiting member, respectively. The first limiting member is provided with a first through hole, and the second limiting member is provided with a second through hole. Along the length direction of the reagent pipette, the projection of the second through hole on the first limiting member does not overlap with the first through hole. The first limiting member, the second limiting member, and the sealing cap form a gas flow cavity on the side of the reagent pipette, and the reagent bottle circulates with the external environment through the first through hole, the gas flow cavity, and the second through hole.

2. The reagent component according to claim 1, characterized in that, The radial area of ​​the first through hole is less than 1 square millimeter.

3. The reagent component according to claim 1, characterized in that, When the second end of the reagent pipette is inside the reagent bottle, the first limiting member abuts against the mouth of the reagent bottle, and the sealing cap is placed on the mouth of the reagent bottle; and during the process of the reagent pipette drawing reagent from the reagent bottle, external gas enters the gas flow chamber through the second through hole, and enters the reagent bottle from the gas flow chamber through the first through hole.

4. The reagent component according to claim 1, characterized in that, The inner wall of the sealing cap includes a protrusion that abuts against the mouth of the reagent bottle. Under the elastic squeezing force of the sealing cap, the protrusion is used to seal the mouth of the reagent bottle.

5. The reagent component according to claim 1, characterized in that, The reagent bottle has a sealing film at the mouth, and the pipette assembly has a puncture part. When the reagent pipette is inserted into the reagent bottle, the puncture part is used to tear the sealing film to form an opening, and the second end of the reagent pipette enters the reagent bottle through the opening.

6. The reagent component according to claim 5, characterized in that, When the second end of the reagent pipette is inside the reagent bottle, the first limiting member contacts the sealing film, and along the length direction of the reagent pipette, the projection of the opening on the first limiting member at least partially overlaps with the first through hole.

7. The reagent component according to claim 1, characterized in that, The reagent assembly also includes a placement bracket, which includes a reagent bottle placement bracket and a pipette placement bracket. The reagent bottle placement bracket is used to place and fix the reagent bottle. The pipette placement bracket is detachably mounted on the reagent bottle placement bracket. When the pipette assembly is detached from the reagent bottle, the pipette placement bracket is used to place the pipette assembly.

8. The reagent component according to claim 1, characterized in that, The bottom of the reagent bottle includes a groove, which is corresponding to the mouth of the reagent bottle, and the second end of the reagent pipette is located in the groove.

9. An analyzer, characterized in that, The device includes a reagent component and a detection component as described in any one of claims 1-8, wherein the detection component and the reagent component are arranged at an interval, and during the sample detection process of the detection component, the reagent component provides the detection component with the reagent in the reagent bottle to assist the detection component in performing sample detection.

10. The analyzer according to claim 9, characterized in that, The analyzer housing is provided with a replacement window, which is correspondingly set to the reagent assembly. After the reagent in the reagent bottle is completely drawn out, the reagent bottle is replaced through the replacement window.

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