Experimental device for inhibiting growth of tumor cells

Abstract

The invention relates to the technical field of tumor research, and discloses an experimental device for inhibiting tumor cell growth, the experimental device comprises an outer box, one side of the outer box is fixedly connected with a side frame, one side of one end of the outer box is rotatably connected with a box door, and the bottom of the outer box is fixedly connected with uniformly distributed supporting seats; a frame door is rotatably connected to the middle of one side of the side frame, a first side door is rotatably connected to the bottom of one end of one side of the side frame, a second side door is rotatably connected to the bottom of the other end of one side of the side frame, an incubator is arranged on the inner wall of the outer box, and sealing rings which are uniformly distributed are rotatably connected to one side of one end of the incubator. A stable environment needed by cell growth can be provided through the humidity pipe, the connecting pipe and the gas tank pipe, when the humidity of the incubator needs to be increased, the gas collecting head is installed, steam is sent into the incubator through the steam pipe and the humidity pipe, and carbon dioxide, oxygen or nitrogen can be injected into the incubator through the gas tank pipe.

Description

Technical Field

[0001] This invention relates to the field of tumor research technology, specifically to an experimental device for inhibiting tumor cell growth. Background Technology

[0002] In the field of tumor research, it is crucial to explore the mechanisms that inhibit tumor cell growth, screen effective anti-tumor drugs, and evaluate the effects of various treatment strategies. However, existing tumor cell experimental devices have many limitations, which seriously restrict the efficient conduct of related research.

[0003] Traditional cell culture containers, such as ordinary culture flasks and culture dishes, cannot accurately simulate the complex microenvironment of tumor cells in vivo when culturing tumor cells. Tumor cells exist in a dynamic environment with multiple interacting factors in vivo, including cell-to-cell communication, cell-extracellular matrix interactions, and specific physicochemical gradients. These key factors cannot be effectively reproduced in conventional culture containers, resulting in significant deviations between in vitro experimental results and actual in vivo conditions. This affects the accurate assessment of tumor cell growth characteristics and drug efficacy.

[0004] In the drug processing stage, there is a lack of optimized designs specifically for drug sensitivity testing of tumor cells. Existing drug addition methods are often not precise or flexible enough, making it difficult to achieve efficient screening of multiple drugs, different concentrations, and complex drug combinations. This not only prolongs the experimental cycle and increases experimental costs, but may also lead to inaccurate and unreliable experimental results due to errors in the drug addition process.

[0005] Among the existing experimental instruments, there is no dedicated device for the overall experiment of inhibiting tumor cell growth. During the experiment, it is impossible to effectively control the concentration of carbon dioxide and temperature, which prevents the tumor cells from achieving the expected effect during drug culture, thus affecting the evaluation of the entire experimental results and resulting in low experimental accuracy. Summary of the Invention

[0006] To address the shortcomings of existing technologies, this invention provides an experimental device for inhibiting tumor cell growth, which solves the problem that the concentration of carbon dioxide and temperature cannot be effectively controlled during experiments, preventing tumor cells from achieving the expected results during drug culture.

[0007] To achieve the above objectives, the present invention provides the following technical solution: an experimental device for inhibiting tumor cell growth, comprising an outer casing, a side frame fixedly connected to one side of the outer casing, a door rotatably connected to one end of the outer casing, evenly distributed support bases fixedly connected to the bottom of the outer casing, a frame door rotatably connected to the middle of one side of the side frame, a first side door rotatably connected to the bottom of one end of one side of the side frame, a second side door rotatably connected to the bottom of the other end of one side of the side frame, an incubator disposed on the inner wall of the outer casing, an evenly distributed sealing ring rotatably connected to one end of the incubator, a viewing window disposed on the inner wall of the sealing ring, and the incubator... The inner wall of the incubator has a filter mesh through one end. A female buckle is fixedly connected to one side of one end of the incubator. A male buckle is fixedly connected to one side of one end of the sealing ring, and the male buckle engages with the female buckle. An isolation component for isolating the incubator from the environment is through one end of the incubator. A modified atmosphere component for supplying gas to the incubator is located at the rear end of the incubator. A distillation component is located at the top center of the inner wall of the side frame. An operating table is fixedly connected to one side of the inner wall of the frame door. A silicone bag is fixedly connected to one side of the outer wall of the frame door. A sealing strip is through one end of the silicone bag. A glove is through one side of the outer wall of the silicone bag.

[0008] Preferably, the isolation assembly includes multiple sliding rods that pass through and are slidably connected to the incubator. One end of the top and bottom sliding rods is fixedly connected to an air curtain machine. A spring is sleeved on the outer ring of each sliding rod. The top and bottom of the air curtain machine are provided with evenly distributed louvers.

[0009] Preferably, the modified atmosphere assembly includes a rear cover, which is fixedly connected to the other end of the incubator. One end of the inner wall of the rear cover is fixedly connected to a uniformly distributed partition, and one side of the inner wall of the rear cover is fixedly connected to a gas distribution plate. One side of the gas distribution plate is permeated and provided with uniformly distributed solenoid valves. The top of the partition is permeated and provided with an ultraviolet disinfection lamp. One end of one side of the rear cover is permeated and fixedly connected to a humidity tube, the middle of one side of the rear cover is permeated and fixedly connected to a connecting pipe, and the other end of one side of the rear cover is permeated and fixedly connected to a gas cylinder tube.

[0010] Preferably, the distillation assembly includes a triangular-mouth flask and a heater. The heater is in contact with the top middle end of the inner wall of the side frame, and the triangular-mouth flask is in contact with the top middle end of the inner wall of the side frame. The flask is disposed on the top inner wall of the heater, and a fractionating head is disposed on the top inner wall of the flask. A gas collecting head is disposed on the other side of the top of the fractionating head. A steam pipe is connected through and fixedly connected to the top of the gas collecting head, and the steam pipe is detachably connected to the humidity pipe.

[0011] Preferably, a steam outlet pipe is connected through and fixedly connected to the other side of the outer wall of the fractionating head. A condenser pipe is sleeved on one end of the outer wall of the steam outlet pipe, and a horn tube is provided at the other end of the condenser pipe. A fixing plug is provided through and installed at the other end of the outer ring of the horn tube, and the fixing plug is slidably connected to the triangular mouth flask.

[0012] Preferably, a water tank is provided on one side of the bottom of the inner wall of the side frame, and an injection port is provided on one side of the top of the water tank. A water pump is provided on the bottom of the inner wall of the water tank, and a water outlet pipe is fixedly connected to the output end of the water pump. A sealing ring is provided through and fixedly connected to the outer wall of the water outlet pipe, and the sealing ring is connected through and fixedly connected to the water tank.

[0013] Preferably, a fixing frame is provided on the other side of the bottom of the inner wall of the side frame, and a gas tank is provided through the top of the fixing frame.

[0014] Preferably, an inlet pipe is connected to the top of the outlet pipe and fixedly connected to it. A sealing plug is provided through the outer wall of the inlet pipe and is slidably connected to the inner wall of the fractionation head.

[0015] This invention provides an experimental device for inhibiting tumor cell growth. It has the following beneficial effects: 1. This invention provides a stable environment for cell growth through a humidity pipe, a connecting pipe, and a gas cylinder pipe. When it is necessary to increase the humidity of the incubator, a gas collecting head is installed, and steam is sent into the incubator through a steam pipe and a humidity pipe. The gas cylinder pipe can inject carbon dioxide, oxygen, or nitrogen into the incubator.

[0016] 2. This invention uses a spring to pop out the air curtain machine and uses louvers to adjust the air outlet angle of the air curtain machine. The air curtain machine, through the high-speed airflow generated by the fan, forms an invisible "air curtain" to prevent unpurified air from entering the incubator, thereby ensuring that the experiments in the clean area are not affected.

[0017] 3. The present invention allows the silicone bag to be opened with a sealing strip, into which the supplies needed to prepare the culture medium are placed. The bag is then placed on the worktable, and the silicone bag is sealed with the sealing strip. The culture medium is then prepared using gloves. The distillation assembly reduces the risk of contamination of the culture medium during preparation. Before use, the distillation assembly is sterilized by irradiating it with ultraviolet light for 30 minutes. Attached Figure Description

[0018] Figure 1 This is a perspective view of the present invention; Figure 2 This is a schematic diagram of the unfolded state of the present invention; Figure 3 This is a schematic diagram of the incubator of the present invention; Figure 4 This is an exploded view of the rear cover of the present invention; Figure 5 This is a schematic diagram of the side frame of the present invention; Figure 6 This is a rear view of the side frame of the present invention; Figure 7 This is a schematic diagram of the distillation assembly of the present invention.

[0019] The components include: 1. Outer casing; 2. Support base; 3. Door; 4. Side frame; 5. Frame door; 6. First side door; 7. Second side door; 8. Silicone bag; 9. Gloves; 10. Sealing strip; 11. Incubator; 12. Air curtain; 13. Filter mesh; 14. Sealing ring; 15. Viewing window; 16. Male snap fastener; 17. Female snap fastener; 18. Louver; 19. Back cover; 20. Partition; 21. Ultraviolet disinfection lamp; 22. Gas distribution plate; 23. Solenoid valve; 24. Humidity pipe; 25. 26. Connecting pipe; 27. Gas cylinder pipe; 28. Slide rod; 29. ​​Spring; 30. Water tank; 31. Liquid filling port; 32. Fixing frame; 33. Gas cylinder; 34. Operating table; 35. Heater; 36. Flask; 37. Fractionating head; 38. Sealing plug; 39. Gas collecting head; 40. Steam outlet pipe; 41. Condenser; 42. Horn tube; 43. Steam pipe; 44. Fixing plug; 45. Triangular mouth flask; 46. Water pump; 47. Water outlet pipe; 48. Sealing ring; 49. Water inlet pipe. Detailed Implementation

[0020] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0021] Example: Please see the appendix Figure 1 -Appendix Figure 7This invention provides an experimental device for inhibiting tumor cell growth, comprising an outer casing 1, a side frame 4 fixedly connected to one side of the outer casing 1, a door 3 rotatably connected to one end of the outer casing 1, evenly distributed support seats 2 fixedly connected to the bottom of the outer casing 1, a frame door 5 rotatably connected to the middle of one side of the side frame 4, a first side door 6 rotatably connected to the bottom of one end of one side of the side frame 4, and a second side door 7 rotatably connected to the bottom of the other end of one side of the side frame 4. An incubator 11 is disposed on the inner wall of the outer casing 1, and evenly distributed support seats 2 are rotatably connected to one end of the incubator 11. A sealing ring 14 has a viewing window 15 on its inner wall. One end of the inner wall of the incubator 11 is penetrated and has a filter mesh 13. One end of the incubator 11 is fixedly connected to a female buckle 17. One end of the sealing ring 14 is fixedly connected to a male buckle 16, and the male buckle 16 engages with the female buckle 17. One end of the incubator 11 is penetrated and has an isolation component for isolating the incubator 11 from the environment. The rear end of the incubator 11 is provided with a modified atmosphere component for supplying gas to the incubator 11. The top of the middle of the inner wall of the side frame 4 is provided with a distillation component.

[0022] The test specimens inside the incubator 11 can be observed through the viewing window 15. Both the viewing window 15 and the interior of the incubator 11 are treated with antibacterial coating to reduce the growth of microorganisms. The sealing ring 14 has good sealing performance to ensure the stability of the internal environment. The filter mesh 13 can filter the gas entering the incubator 11 and also prevent some microorganisms from entering the back cover 19. The male buckle 16 and female buckle 17 facilitate the opening or closing of the viewing window 15.

[0023] Please see the appendix Figure 2 -Appendix Figure 4 The isolation assembly includes multiple slide rods 27, which are connected to the incubator 11 through and in a sliding manner. One end of the top slide rod 27 and the bottom slide rod 27 are fixedly connected to an air curtain machine 12. The outer ring of the slide rod 27 is fitted with a spring 28. The top and bottom of the air curtain machine 12 are provided with evenly distributed louvers 18.

[0024] Open the chamber door 3, and the air curtain machine 12 will pop out by the spring 28. The air outlet angle of the air curtain machine 12 will be adjusted by the louvers 18. The air curtain machine 12 will form an invisible "air curtain" through the high-speed airflow generated by the fan, preventing unpurified air from entering the incubator 11, thereby ensuring that the experiments in the clean area are not affected.

[0025] Please see the appendix Figure 4The modified atmosphere assembly includes a rear cover 19, which is fixedly connected to the other end of the incubator 11. One end of the inner wall of the rear cover 19 is fixedly connected to a uniformly distributed partition 20. One side of the inner wall of the rear cover 19 is fixedly connected to a gas distribution plate 22. One side of the gas distribution plate 22 is provided with a uniformly distributed solenoid valve 23. The top of the partition 20 is provided with an ultraviolet disinfection lamp 21. One end of one side of the rear cover 19 is fixedly connected to a humidity tube 24. One middle of one side of the rear cover 19 is fixedly connected to a connecting pipe 25. The other end of one side of the rear cover 19 is fixedly connected to a gas cylinder pipe 26.

[0026] External gas can be delivered into the gas distribution plate 22 through the humidity tube 24, connecting tube 25, and gas cylinder tube 26. By opening the solenoid valves 23 at different positions, different gases can be injected into different positions of the incubator 11. The output ends of the humidity tube 24, connecting tube 25, and gas cylinder tube 26 are all equipped with one-way valves to prevent gas mixing. After the experiment, the ultraviolet disinfection lamp 21 can be turned on to disinfect the inside of the back cover 19. Ultraviolet light can destroy the DNA structure of microorganisms, thereby killing them and preventing microbial contamination from interfering with cell growth and experimental results. The humidity tube 24, connecting tube 25, and gas cylinder tube 26 can provide a stable environment required for cell growth, including a suitable temperature (generally 37°C), humidity (generally around 90% to 95%), and carbon dioxide concentration (usually 5%).

[0027] Please see the appendix Figure 5 -Appendix Figure 7 The distillation assembly includes a triangular-mouth flask 44 and a heater 34. The heater 34 contacts the top middle of the inner wall of the side frame 4 at one end, and the triangular-mouth flask 44 contacts the top middle of the inner wall of the side frame 4 at the other end. A flask 35 is mounted on the top inner wall of the heater 34, and a fractionating head 36 is mounted on the top inner wall of the flask 35. A gas collecting head 38 is mounted on the other side of the top of the fractionating head 36. A vapor pipe 42 is connected through and fixedly connected to the top of the gas collecting head 38, and the vapor pipe 42 is detachably connected to the humidity pipe 24. The other side of the outer wall of the fractionating head 36... A steam outlet pipe 39 is connected through and fixedly connected to the side. A condenser pipe 40 is fitted on one end of the outer wall of the steam outlet pipe 39. A horn tube 41 is provided on the other end of the condenser pipe 40. A fixing plug 43 is provided through and installed on the other end of the outer ring of the horn tube 41. The fixing plug 43 is slidably connected to the triangular mouth flask 44. An operating table 33 is connected through and fixedly connected to one side of the inner wall of the frame door 5. A silicone bag 8 is fixedly connected to one side of the outer wall of the frame door 5. A sealing strip 10 is provided through and installed on one end of the silicone bag 8. A glove 9 is provided through and installed on one side of the outer wall of the silicone bag 8.

[0028] The silicone bag 8 can be opened through the sealing strip 10. Place the necessary materials for preparing the culture medium into the silicone bag 8 and place it on the operating table 33. Then seal the silicone bag 8 with the sealing strip 10. Next, using gloves 9, prepare the culture medium by slowly adding the culture medium powder to an appropriate amount of ultrapure water (made through a distillation unit) and stirring with a magnetic stirrer until completely dissolved. Filter the culture medium to sterilize it using a 0.22μm or 0.45μm filter. Add fetal bovine serum to the sterilized culture medium. Turn on the water pump 45, which pumps the purified water from the water tank 29 through the outlet pipe 46 and the inlet pipe 48 into the flask 35. Then, turn on heater 34 to heat flask 35 and pure water to boiling point, turning the pure water into steam. Then, let the steam pass through condenser 40 to cool and condense into distilled water, which enters triangular flask 44. When it is not necessary to increase the humidity of incubator 11, replace the collecting head 38 with a stopper. When it is necessary to increase the humidity of incubator 11, install the collecting head 38 and send the steam into incubator 11 through steam pipe 42 and humidity pipe 24. The distillation assembly reduces the contamination of the culture medium during preparation. Before use, the distillation assembly is sterilized by irradiation with ultraviolet light for 30 minutes.

[0029] Please see the appendix Figure 5 -Appendix Figure 6 A water tank 29 is provided on one side of the bottom of the inner wall of the side frame 4. A liquid injection port 30 is provided on one side of the top of the water tank 29. A water pump 45 is provided on the bottom of the inner wall of the water tank 29. A water outlet pipe 46 is fixedly connected to the output end of the water pump 45. A sealing ring 47 is provided through and fixedly connected to the outer wall of the water outlet pipe 46. The sealing ring 47 is also provided through and fixedly connected to the water tank 29. A water inlet pipe 48 is provided through and fixedly connected to the top of the water outlet pipe 46. A sealing plug 37 is provided through and installed on the outer wall of the water inlet pipe 48. The sealing plug 37 is slidably connected to the inner wall of the fractionation head 36. Pure water can be injected into the water tank 29 through the injection port 30. The water pump 45 can send the pure water into the flask through the water outlet pipe 46. The sealing ring 47 can prevent leakage, and the sealing plug 37 can prevent steam leakage.

[0030] Please see the appendix Figure 6 A fixing frame 31 is provided on the other side of the bottom of the inner wall of the side frame 4, and a gas tank 32 is provided through the top of the fixing frame 31.

[0031] The side frame 4 can be opened through the second side door 7, and the gas tank 32 can be placed through the fixing bracket 31. Carbon dioxide, oxygen or nitrogen can be injected into the incubator 11 through the gas tank pipe 26, depending on the gas in the gas tank 32.

[0032] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An experimental apparatus for inhibiting tumor cell growth, comprising an outer casing (1), characterized in that, A side frame (4) is fixedly connected to one side of the outer box (1), and a door (3) is rotatably connected to one end of the outer box (1). Evenly distributed support seats (2) are fixedly connected to the bottom of the outer box (1). A frame door (5) is rotatably connected to the middle of one side of the side frame (4). A first side door (6) is rotatably connected to the bottom of one end of one side of the side frame (4), and a second side door (7) is rotatably connected to the bottom of the other end of one side of the side frame (4). An incubator (11) is installed on the inner wall of the outer box (1). Evenly distributed sealing rings (14) are rotatably connected to one end of the incubator (11). A viewing window (15) is provided on the inner wall of the sealing ring (14). A filter mesh (13) is provided through one end of the inner wall of the incubator (11). 1) A female buckle (17) is fixedly connected to one side of one end of the sealing ring (14), and a male buckle (16) is fixedly connected to one side of one end of the sealing ring (14), and the male buckle (16) and the female buckle (17) are engaged. One end of the incubator (11) is provided with an isolation component for isolating the incubator (11) from the environment. The rear end of the incubator (11) is provided with a modified atmosphere component for supplying gas to the incubator (11). A distillation component is provided at the top of the middle of the inner wall of the side frame (4). An operating table (33) is fixedly connected to one side of the inner wall of the frame door (5). A silicone bag (8) is fixedly connected to one side of the outer wall of the frame door (5). A sealing strip (10) is provided to one end of the silicone bag (8). A glove (9) is provided to one side of the outer wall of the silicone bag (8).

2. The experimental apparatus for inhibiting tumor cell growth according to claim 1, characterized in that, The isolation assembly includes multiple slide rods (27), which are connected to the incubator (11) through and slidably. One end of the top slide rod (27) and the bottom slide rod (27) are fixedly connected to an air curtain machine (12). The outer ring of the slide rod (27) is fitted with a spring (28). The top and bottom of the air curtain machine (12) are provided with evenly distributed louvers (18).

3. The experimental apparatus for inhibiting tumor cell growth according to claim 1, characterized in that, The modified atmosphere assembly includes a back cover (19), which is fixedly connected to the other end of the incubator (11). One end of the inner wall of the back cover (19) is fixedly connected to a uniformly distributed partition (20). One side of the inner wall of the back cover (19) is fixedly connected to a gas distribution plate (22). One side of the gas distribution plate (22) is provided with a uniformly distributed solenoid valve (23). The top of the partition (20) is provided with an ultraviolet disinfection lamp (21). One end of one side of the back cover (19) is connected with a humidity tube (24). The middle of one side of the back cover (19) is connected with a connecting tube (25). The other end of one side of the back cover (19) is connected with a gas cylinder tube (26).

4. The experimental apparatus for inhibiting tumor cell growth according to claim 1, characterized in that, The distillation assembly includes a triangular mouth flask (44) and a heater (34). The heater (34) is in contact with the top middle of the inner wall of the side frame (4), and the triangular mouth flask (44) is in contact with the top middle of the inner wall of the side frame (4). A flask (35) is provided on the top inner wall of the heater (34). A fractionating head (36) is provided on the top inner wall of the flask (35). A gas collecting head (38) is provided on the other side of the top of the fractionating head (36). A steam pipe (42) is connected through and fixedly connected to the top of the gas collecting head (38), and the steam pipe (42) is detachably connected to the humidity pipe (24).

5. The experimental apparatus for inhibiting tumor cell growth according to claim 4, characterized in that, A steam outlet pipe (39) is connected to the other side of the outer wall of the fractionating head (36). A condenser pipe (40) is fitted on one end of the outer wall of the steam outlet pipe (39). A horn tube (41) is provided at the other end of the condenser pipe (40). A fixing plug (43) is provided at the other end of the outer ring of the horn tube (41), and the fixing plug (43) is slidably connected to the triangular mouth flask (44).

6. The experimental apparatus for inhibiting tumor cell growth according to claim 1, characterized in that, A water tank (29) is provided on one side of the bottom of the inner wall of the side frame (4). An injection port (30) is provided on one side of the top of the water tank (29). A water pump (45) is provided on the bottom of the inner wall of the water tank (29). A water outlet pipe (46) is fixedly connected to the output end of the water pump (45). A sealing ring (47) is fixedly connected through the outer wall of the water outlet pipe (46), and the sealing ring (47) is fixedly connected through the water tank (29).

7. The experimental apparatus for inhibiting tumor cell growth according to claim 1, characterized in that, A fixing frame (31) is provided on the other side of the bottom of the inner wall of the side frame (4), and a gas tank (32) is provided through the top of the fixing frame (31).

8. The experimental apparatus for inhibiting tumor cell growth according to claim 6, characterized in that, The top of the outlet pipe (46) is connected to the inlet pipe (48), and the outer wall of the inlet pipe (48) is connected to the sealing plug (37), and the sealing plug (37) is slidably connected to the inner wall of the fractionating head (36).

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