Repellant for effectively protecting pine tree from being infected by pine wood nematode disease and laying method thereof

Abstract

The invention relates to the technical field of pest control, in particular to a repellent for effectively protecting pine trees from being infected by pine wood nematode diseases and a laying method of the repellent. A method for laying repellents for effectively protecting pine trees from being infected by pine wood nematode diseases comprises the following steps that a deep forest is subjected to circular area division, and therefore a plurality of circular areas are set; arranging a repellent at the central point of each circular area; in each circular area, arranging a monochamus alternatus trapper in the direction of circumferential diffusion from the center to the circular area by taking the repellent arranged at the central point as a starting point, and luring the monochamus alternatus into the trapper; the distance between the repellent and the monochamus alternatus trapper is 0-40 m; the invention discloses a repellent for effectively protecting pine trees from being infected by pine wood nematode diseases and a laying method thereof, and the pine trees are effectively protected from being infected by the pine wood nematode diseases by optimizing the suspension distance between the repellent and a monochamus alternatus trap and the interval time of replacing the repellent.

Description

Technical Field

[0001] This application relates to the technical field of pest control, and in particular to a repellent and its application method for effectively protecting pine trees from pine wilt disease. Background Technology

[0002] Pine wilt disease is a devastating disease of pine trees, commonly known as pine wilt. It is highly virulent, has a long incubation period, and infected pine trees can wither and die within months, earning it the reputation of being the "cancer" of pine trees. Currently, Asia is the region most severely affected by pine wilt disease.

[0003] Judging from the development trend in recent years, the pine wilt disease epidemic is not optimistic and is showing an intensifying trend. Many famous scenic spots, world natural and cultural heritage sites, ancient pines, and key ecological areas are facing disaster crises, seriously threatening the development of pine forest areas and related forestry economies. It is the most dangerous number one forest disease.

[0004] Pine wilt disease is primarily transmitted by the pine sawyer beetle. Currently, prevention and protection of famous and ancient pines from pine wilt nematode infection mainly rely on trunk injection of pesticides, pesticides to control the pine sawyer beetle, and physical trapping of the beetle. However, because famous and ancient pines are important heritage sites with immense cultural value, the current control methods may increase the risk of further infection, directly or indirectly causing secondary damage to these trees. Furthermore, publicly available methods for protecting pine trees using repellents do not consider the practical effectiveness and cost of application.

[0005] Therefore, optimizing the distance between the repellent and the pine wilt disease trap, as well as the interval for replacing the repellent, to effectively protect famous and ancient pines from pine wilt disease has become an urgent problem to be solved. Summary of the Invention

[0006] To address the shortcomings of existing technologies, this application provides an effective repellent for protecting pine trees from pine wilt disease and a method for its application. When using the repellent, simply hang it on the pine tree to be protected or on surrounding trees and buildings to prevent the pine sawyer beetle from feeding on the protected pine tree and thus spreading pine wilt disease.

[0007] Firstly, this application provides a method for applying a repellent to effectively protect pine trees from pine wilt disease, employing the following technical solution: A method for applying a repellent to effectively protect pine trees from pine wilt disease includes the following steps: The forest is divided into circular areas, thus establishing multiple circular areas; Place a repellent agent at the center point of each circular area; In each circular region, the repelling agent set at the center point is taken as the starting point, the pheromone trap is set in the direction of the diffusion from the center to the circumference of the circular region, and the M. alternatus is attracted into the pheromone trap.

[0008] Preferably, the M. alternatus pheromone trap is provided with a M. alternatus attractant.

[0009] By adopting the above technical solution, the present application delimits a plurality of circular regions, expels the M. alternatus into the M. alternatus pheromone trap, can more thoroughly trap the M. alternatus in the region, saves the cost of the pesticide, the economic cost and the labor cost, at the same time, makes the repelling effect of the repelling agent optimal, thereby effectively protects the pine trees from the pine wood nematode disease.

[0010] Preferably, the distance between the repelling agent and the M. alternatus pheromone trap is 0-40 meters.

[0011] Further preferably, the distance between the repelling agent and the M. alternatus pheromone trap is 0-20 meters.

[0012] By adopting the above technical solution, by limiting the distance between the repelling agent and the M. alternatus pheromone trap, the repelling effect of the repelling agent is optimal while saving the cost of the pesticide and the labor cost.

[0013] Preferably, the repelling agent is replaced, and the steps are as follows: the repelling agent is replaced once every 15-45 days.

[0014] Further preferably, the interval days are 15-30 days.

[0015] By adopting the above technical solution, by limiting the interval time of replacing the repelling agent, the repelling effect of the repelling agent is optimal while saving the cost of the pesticide and the labor cost.

[0016] The present application limits the distance between the repelling agent and the M. alternatus pheromone trap and the interval time of replacing the repelling agent, avoids the subjective idea that the smaller the hanging interval of the repelling agent is and the faster the replacement frequency is, the better the protection effect is, and reduces the economic cost and the waste of the pesticide.

[0017] Preferably, the repelling agent comprises the following raw material components in mass fraction: 4-allyl anisole 10-30 parts, beta-phenylacrylaldehyde 10-30 parts, ethanol 60-80 parts, Tween-80 2-8 parts, and linalool 10-20 parts.

[0018] Preferably, the repelling agent further comprises eugenol 10-16 parts by mass.

[0019] Preferably, the repelling agent further comprises benzyl acetate 5-15 parts by mass and vanillin 5-15 parts by mass.

[0020] Preferably, the repellent further comprises stearic acid 3.5-4.5 parts by mass and isopropyl myristate 2.5-3.5 parts by mass.

[0021] By adopting the technical scheme, the repellent effect of the repellent can be improved significantly through the improvement of raw materials and the synergistic effect between the raw materials.

[0022] Preferably, the repellent further comprises an encapsulating agent 30-50 parts by mass; the encapsulating agent comprises the following raw material components in parts by mass: gelatin 30-50 parts, quaternary ammonium chitosan 5-15 parts, glycerol 7-13 parts, linalool 1.6-2.5 parts, Tween-80 0.45-0.6 parts, and corn starch 10-15 parts.

[0023] By adopting the technical scheme, the drug is wrapped by the film-forming agent, and a dense protective film is formed on the surface of the drug, which can effectively reduce the drug dosage fluctuation, enhance the drug efficacy, improve the drug utilization efficiency, and reduce the potential impact of the drug on the environment; the film-forming agent can make the drug release slowly, prolong the action time of the active ingredients, reduce the number of drug applications, and reduce the total amount of drug applications; and the degradation of the active ingredients caused by environmental factors can be reduced or even avoided.

[0024] In the application, the quaternary ammonium chitosan and linalool are compounded, the positive charge of the quaternary ammonium chitosan is combined with the volatile components of linalool, the double effects of positive charge interference and odor interference are formed, the repellent effect of the repellent can be improved effectively; and the synergistic effect between the quaternary ammonium chitosan and the gelatin, corn starch, and linalool can form a more dense protective layer.

[0025] Preferably, the preparation method of the encapsulating agent comprises the following steps: The gelatin and the quaternary ammonium chitosan are mixed, the glycerol is added and mixed, the first part of linalool is added and mixed, then the Tween-80 is added and mixed to obtain a compound; The corn starch is mixed with water, heated at 90-100℃ for 1-2 hours, cooled to 20-30℃, the second part of linalool is added and mixed, heated to 30-50℃, and then the compound is added and mixed to obtain the encapsulating agent; The mass ratio of the first part of linalool to the second part of linalool is (1-2):(0.1-1.5).

[0026] By adopting the technical scheme, the application uses linalool, quaternized chitosan and gelatin as raw materials, uses surfactant Tween-80 to coat linalool to form an emulsion, and then adds a mixture obtained by blending cannamycin starch and linalool to prepare a film coating agent with good stability.

[0027] The application discloses a repellent for effectively protecting pines from pine wood nematode disease infection and a laying method thereof, and has the following advantages: 1. no chemical pesticide is used, the repellent itself cannot kill the beetles, the special smell of the repellent can make the beetles escape or be driven away, and no harm is caused to the protected pines or other non-target organisms; 2. the use of the monochamus alternatus trap alone causes the beetles to gather, thereby increasing the risk of spreading the pine wood nematode disease; 3. in the prior art, the drug cannot kill all the beetles, and the surviving monochamus alternatus still causes the infection risk of the pine wood nematode disease; 4. the hanging distance between the repellent and the monochamus alternatus trap and the interval time for replacing the repellent are optimized, the subjective idea that the smaller the hanging distance and the faster the replacement frequency are, the better the protection effect is achieved is avoided, and the economic cost of application and the waste of the drug are reduced.

[0028] In conclusion, the application has at least one of the following beneficial technical effects: The application discloses a repellent for effectively protecting pines from pine wood nematode disease infection and a laying method thereof, and the hanging distance between the repellent and the monochamus alternatus trap and the interval time for replacing the repellent are limited, so that the repellent prevents and protects the pines from the infection of the pine wood nematode disease, and the repellent effect of the repellent is effectively improved, and the economic cost of use is saved. In the application, the raw material of the repellent is improved, the film coating agent added on the surface of the drug forms a dense protective layer, the repellent effect of the repellent is effectively improved, and the service life of the drug is prolonged. DETAILED DESCRIPTION

[0029] The technical scheme of the application is further illustrated by specific examples below, and the specific examples do not represent a limitation on the protection scope of the application; some non-essential modifications and adjustments made by others according to the concept of the application still belong to the protection scope of the application.

[0030] The raw materials involved in the application are all commercially available products, and the specific types are as follows: Indica rice is purchased from Hefei Jin Yuchao Food Co., Ltd. Gelatin is purchased from Hebei Huafeng Adhesive Co., Ltd. Quaternized chitosan with a degree of substitution of 90% is purchased from Qingdao Haipu Biotechnology Co., Ltd. Glycerol, purchased from Jiangsu Xinhua Glycerol Technology Co., Ltd. 4-allylanisole, Chinese alias, estragole; beta-phenylpropenal, Chinese alias, cinnamaldehyde; Monochamus alternatus trap, Bursaphelenchus xylophilus medium Monochamus alternatus photoelectric intelligent trap, model SYYZ-8035A, SYYZ-8035B, purchased from Nanjing Shengxing Pest Control Technology Co., Ltd. Monochamus alternatus attractant, model Bursaphelenchus xylophilus medium Monochamus alternatus attractant SY3, purchased from Nanjing Shengxing Pest Control Technology Co., Ltd. The application will be further described in detail below in combination with examples and comparative examples.

[0031] Raw material sources: The preparation method of indica rice starch is as follows: (1) The indica rice is ground into powder with a particle size not greater than 80 mesh, and defatted according to a petroleum ether ratio of 1:2 (g / mL); (2) The defatted sample is dispersed in 0.2% NaOH solution (1:4, g / mL) for soaking, the supernatant is discarded, and then the indica rice powder is washed multiple times until white starch is obtained in the precipitate; (3) Drying in an oven at 40°C, and grinding to pass through a 100 mesh sieve to obtain indica rice starch.

[0032] Preparation Example 1: Preparation of the coating agent: The coating agent comprises the following raw material components: gelatin 40 kg, quaternary ammonium chitosan 10 kg, glycerol 10 kg, linalool 2 kg, Tween-80 0.5 kg, and canna starch 13 kg.

[0033] The preparation method of the coating agent is as follows: After mixing the gelatin and quaternary ammonium chitosan uniformly, glycerol is added, and mixing is carried out at 60°C for 1 hour. Then the first portion of linalool is added and mixed for 10 minutes, and then Tween-80 is added and mixed for 20 minutes to obtain a complex; Water 500 mL is added to the canna starch for mixing, heated at 95°C for 1.5 hours, then cooled to 25°C, the second portion of linalool is added, mixed at 40°C for 30 minutes, and then the complex is added and mixed for 30 minutes to obtain the coating agent.

[0034] The mass ratio of the first portion of linalool to the second portion of linalool is 1.5:0.5.

[0035] Preparation Example 2: Preparation of the coating agent: The coating agent comprises the following raw material components: gelatin 30 kg, quaternary ammonium chitosan 5 kg, glycerol 7 kg, linalool 1.6 kg, Tween-80 0.45 kg, and corn starch 10 kg.

[0036] The preparation method of the coating agent comprises the following steps: After the gelatin and the quaternary ammonium chitosan are uniformly mixed, the glycerol is added, and mixing is performed at 60°C for 1 hour. Then, the first portion of linalool is added, and mixing is performed for 10 minutes. Then, the Tween-80 is added, and mixing is performed for 20 minutes to obtain a compound. The corn starch is mixed with water 500 mL, and heating is performed at 95°C for 1.5 hours. Then, the temperature is lowered to 25°C, the second portion of linalool is added, and mixing is performed at 40°C for 30 minutes. Then, the compound is added, and mixing is performed for 30 minutes to obtain the coating agent.

[0037] The mass ratio of the first portion of linalool to the second portion of linalool is 1:0.6.

[0038] Preparation Example 3: Preparation of the coating agent: The coating agent comprises the following raw material components: gelatin 50 kg, quaternary ammonium chitosan 15 kg, glycerol 13 kg, linalool 2.5 kg, Tween-80 0.6 kg, and corn starch 15 kg.

[0039] The preparation method of the coating agent comprises the following steps: After the gelatin and the quaternary ammonium chitosan are uniformly mixed, the glycerol is added, and mixing is performed at 60°C for 1 hour. Then, the first portion of linalool is added, and mixing is performed for 10 minutes. Then, the Tween-80 is added, and mixing is performed for 20 minutes to obtain a compound. The corn starch is mixed with water 500 mL, and heating is performed at 95°C for 1.5 hours. Then, the temperature is lowered to 25°C, the second portion of linalool is added, and mixing is performed at 40°C for 30 minutes. Then, the compound is added, and mixing is performed for 30 minutes to obtain the coating agent.

[0040] The mass ratio of the first portion of linalool to the second portion of linalool is 2:0.5.

[0041] Example 1: The preparation method of the liquid repellent agent comprises the following steps: After the 4-allyl anisole 20 kg, beta-phenylpropenyl aldehyde 20 kg, anhydrous ethanol 70 kg, Tween-80 5 kg, and linalool 15 kg are stirred and uniformly mixed, a liquid repellent agent is obtained.

[0042] Example 2: The preparation method of the liquid repellent agent comprises the following steps: Stir 4-allylanisole 10 kg, beta-phenylpropenal 10 kg, anhydrous ethanol 60 kg, Tween-80 2 kg, linalool 10 kg and mix well to obtain liquid repellent.

[0043] Example 3: The preparation method of liquid repellent is as follows: Stir 4-allylanisole 30 kg, beta-phenylpropenal 30 kg, anhydrous ethanol 80 kg, Tween-80 8 kg, linalool 20 kg and mix well to obtain liquid repellent.

[0044] Example 4: The difference from Example 1 is that the liquid repellent further comprises eugenol 13 kg.

[0045] Example 5: The difference from Example 2 is that the liquid repellent further comprises eugenol 10 kg.

[0046] Example 6: The difference from Example 3 is that the liquid repellent further comprises eugenol 16 kg.

[0047] Example 7: The difference from Example 1 is that the liquid repellent further comprises benzyl acetate 10 kg, vanillin 10 kg.

[0048] Example 8: The difference from Example 2 is that the liquid repellent further comprises benzyl acetate 5 kg, vanillin 5 kg.

[0049] Example 9: The difference from Example 3 is that the liquid repellent further comprises benzyl acetate 15 kg, vanillin 15 kg.

[0050] Example 10: The difference from Example 4 is that the liquid repellent further comprises encapsulant 40 kg.

[0051] The encapsulant is prepared by Preparation Example 1.

[0052] Example 11: The difference from Example 5 is that the liquid repellent further comprises encapsulant 30 kg.

[0053] The encapsulant is prepared by Preparation Example 2.

[0054] Example 12: The difference from Example 6 is that the liquid repellent further comprises encapsulant 50 kg.

[0055] The film coating agent is prepared according to Preparation Example 1.

[0056] Example 13: The difference from Example 10 is that the liquid repellent agent further comprises a film coating agent 30 kg.

[0057] Example 14: The difference from Example 10 is that the liquid repellent agent further comprises a film coating agent 50 kg.

[0058] Example 15: The difference from Example 7 is that the liquid repellent agent further comprises a film coating agent 40 kg.

[0059] The film coating agent is prepared according to Preparation Example 1.

[0060] Example 16: The difference from Example 8 is that the liquid repellent agent further comprises a film coating agent 30 kg.

[0061] The film coating agent is prepared according to Preparation Example 2.

[0062] Example 17: The difference from Example 9 is that the liquid repellent agent further comprises a film coating agent 50 kg.

[0063] The film coating agent is prepared according to Preparation Example 3.

[0064] Example 18: The difference from Example 15 is that the liquid repellent agent further comprises a film coating agent 30 kg.

[0065] Example 19: The difference from Example 15 is that the liquid repellent agent further comprises a film coating agent 50 kg.

[0066] Example 20: The preparation method of the solid repellent agent is as follows: After stirring, 4-allylanisole 20 kg, beta-phenylpropenylaldehyde 20 kg, anhydrous ethanol 70 kg, Tween-80 5 kg, linalool 15 kg, eugenol 13 kg are uniformly mixed; Then, stearic acid 4 kg and isopropyl myristate 3 kg are added and mixed, and then heated to 60℃. Under stirring, 10 mL of alkali solution (10% sodium hydroxide solution) is added, and the stirring is not stopped to prepare the solid repellent agent.

[0067] Example 21: The preparation method of the solid repellent agent is as follows: Stir 4-allylanisole 10 kg, beta-phenylpropenal 10 kg, anhydrous ethanol 60 kg, Tween-80 2 kg, linalool 10 kg, eugenol 10 kg, and mix uniformly; Add stearic acid 3.5 kg and isopropyl cetylate 2.5 kg, mix, heat to 60°C, and under stirring, add alkali solution (10% sodium hydroxide solution) 10 mL, and under continuous stirring, obtain the solid repellent.

[0068] Example 22: The preparation method of the solid repellent is as follows: Stir 4-allylanisole 30 kg, beta-phenylpropenal 30 kg, anhydrous ethanol 80 kg, Tween-80 8 kg, linalool 20 kg, eugenol 16 kg, and mix uniformly; Add stearic acid 4.5 kg and isopropyl cetylate 3.5 kg, mix, heat to 60°C, and under stirring, add alkali solution (10% sodium hydroxide solution) 10 mL, and under continuous stirring, obtain the solid repellent.

[0069] Example 23: The difference from Example 20 is that the solid repellent further comprises 40 kg of film coating agent.

[0070] The film coating agent is prepared by Preparation Example 1.

[0071] Example 24: The difference from Example 21 is that the solid repellent further comprises 30 kg of film coating agent.

[0072] The film coating agent is prepared by Preparation Example 2.

[0073] Example 25: The difference from Example 22 is that the solid repellent further comprises 50 kg of film coating agent.

[0074] The film coating agent is prepared by Preparation Example 3.

[0075] Example 26: The difference from Example 23 is that the solid repellent further comprises 30 kg of film coating agent.

[0076] Example 27: The difference from Example 23 is that the solid repellent further comprises 50 kg of film coating agent.

[0077] Example 28: The preparation method of the solid repellent is as follows: Stir 4-allylanisole 20 kg, beta-phenylpropenal 20 kg, anhydrous ethanol 70 kg, Tween-80 5 kg, linalool 15 kg, benzyl acetate 10 kg, vanillin 10 kg, and mix uniformly; Then add stearic acid 4 kg and isopropyl cetylate 3 kg, mix, heat to 60°C, and under stirring, add alkali solution (10% sodium hydroxide solution) 10 mL, and under continuous stirring, obtain the solid repellent.

[0078] Example 29: The preparation method of the solid repellent is as follows: Stir 4-allylanisole 10 kg, beta-phenylpropenal 10 kg, anhydrous ethanol 60 kg, Tween-80 2 kg, linalool 10 kg, benzyl acetate 5 kg, and vanillin 5 kg, and mix uniformly; Then add stearic acid 3.5 kg and isopropyl cetylate 2.5 kg, mix, heat to 60°C, and under stirring, add alkali solution (10% sodium hydroxide solution) 10 mL, and under continuous stirring, obtain the solid repellent.

[0079] Example 30: The preparation method of the solid repellent is as follows: Stir 4-allylanisole 30 kg, beta-phenylpropenal 30 kg, anhydrous ethanol 80 kg, Tween-80 8 kg, linalool 20 kg, benzyl acetate 15 kg, and vanillin 15 kg, and mix uniformly; Then add stearic acid 4.5 kg and isopropyl cetylate 3.5 kg, mix, heat to 60°C, and under stirring, add alkali solution (10% sodium hydroxide solution) 10 mL, and under continuous stirring, obtain the solid repellent.

[0080] Example 31: The difference from Example 28 is that the solid repellent further comprises 40 kg of the coating agent.

[0081] The coating agent is prepared according to Preparation Example 1.

[0082] Example 32: The difference from Example 29 is that the solid repellent further comprises 30 kg of the coating agent.

[0083] The coating agent is prepared according to Preparation Example 2.

[0084] Example 33: The difference from Example 30 is that the solid repellent further comprises 50 kg of the coating agent.

[0085] The coating agent is prepared according to Preparation Example 3.

[0086] Example 34: The difference from Example 31 is that the solid repellent further comprises a film- forming agent 30 kg.

[0087] Example 35: The difference from Example 31 is that the solid repellent further comprises a film- forming agent 50 kg.

[0088] Comparative Example 1: The difference from Example 10 is that the liquid repellent further comprises a film- forming agent 29 kg.

[0089] Comparative Example 2: The difference from Example 10 is that the liquid repellent further comprises a film- forming agent 51 kg.

[0090] Comparative Example 3: The difference from Example 15 is that the liquid repellent further comprises a film- forming agent 29 kg.

[0091] Comparative Example 4: The difference from Example 15 is that the liquid repellent further comprises a film- forming agent 51 kg.

[0092] Comparative Example 5: The difference from Example 23 is that the solid repellent further comprises a film- forming agent 29 kg.

[0093] Comparative Example 6: The difference from Example 23 is that the solid repellent further comprises a film- forming agent 51 kg.

[0094] Comparative Example 7: The difference from Example 31 is that the solid repellent further comprises a film- forming agent 29 kg.

[0095] Comparative Example 8: The difference from Example 31 is that the solid repellent further comprises a film- forming agent 51 kg.

[0096] Performance Test: 1. Laboratory repellent test 1.1. Preparation of test insects Healthy adults of M. alternatus (male:female ratio 1 : 1) were collected and acclimated for 3-5 days under laboratory conditions of 25 ± 1 °C, 70% ± 5% relative humidity, and a photoperiod of 14L: 10D. Individuals with consistent activity and similar body size were selected as the source of test insects.

[0097] 1.2. Preparation of repellent Liquid repellent: The repellent prepared in Examples 1-19 and Comparative Examples 1-4 was dissolved in ethanol to prepare a repellent having a concentration of 0.6%.

[0098] Solid repellent: 20 g of the solid repellent prepared in Examples 20-35 and Comparative Examples 5-8 was weighed.

[0099] 1.3, Experimental apparatus A three-chamber repellent box (3 m x 3 m x 3 m) was used; chamber A of the repellent box was the treatment zone, and chamber C was the control zone. The airflow velocity in the box was maintained at 0.3-0.5 m / s, the temperature was 25±2°C, and the humidity was 70%±5%; 1.4, Experimental material treatment Fresh pine branches were cut into 14 cm long sections and hung in chambers A and C of the repellent box, respectively. The repellent (liquid repellent or solid repellent) was hung in the treatment zone, and the repellent (liquid repellent or solid repellent) was 10 cm away from the test branches. A blank carrier was hung in the control zone, and the blank carrier was 10 cm away from the test branches. 1.5, Experimental procedure One male and one female M. alternatus adult was placed in chamber B of the repellent box for a 7-day observation. The branches were removed daily, and the feeding patches were transferred onto sulfuric acid paper and the feeding area was calculated. At the same time, the health of the beetles was observed, and any deaths were promptly replenished.

[0100] Each test was set up with 10 replicates.

[0101] 1.6, Repellent effect calculation The calculation method of the repellent effect of the repellent was as follows: the repellent effect of the repellent was calculated according to the size of the feeding area during the feeding process, and the calculation model was as follows: Model: Repellency rate (RI) = (CK-T) / CK x 100; Note: In the calculation model, CK is the feeding area of the beetles in the control zone, and T is the feeding area of the beetles in the treatment zone.

[0102] Table 1 Repellency rate test results Based on the test results of Examples 1-3 and Examples 4-6, it can be seen that the repellent effect of Examples 4-6 is better than that of Examples 1-3, which indicates that the improvement of the components of the repellent and the synergistic effect between the added eugenol and each raw material further improve the repellent effect of the repellent on M. alternatus.

[0103] In combination with the detection results of Examples 1-3 and Examples 7-9, it can be seen that the repellent effect of Examples 7-9 is better than that of Examples 1-3, which indicates that the composition of the repellent is improved, and the synergistic effect between the added phenyl methyl acetate, vanillin and each raw material further improves the repellent effect of the repellent on the monochamus alternatus.

[0104] In combination with the detection results of Examples 4-6 and Examples 10-12, it can be seen that the repellent effect of Examples 10-12 is better than that of Examples 4-6, which indicates that the composition of the repellent is improved, and the added coating agent effectively improves the repellent effect of the repellent on the monochamus alternatus.

[0105] In combination with the detection results of Example 10, Example 13, Example 14, Comparative Example 1 and Comparative Example 2, it can be seen that the addition amount of the coating agent has a certain influence on the repellent effect of the repellent, and when the addition amount of the coating agent is 30-50 kg, the repellent effect of the repellent on the monochamus alternatus is best.

[0106] In combination with the detection results of Examples 7-9 and Examples 15-17, it can be seen that the repellent effect of Examples 15-17 is better than that of Examples 7-9, which indicates that the composition of the repellent is improved, and the added coating agent effectively improves the repellent effect of the repellent on the monochamus alternatus.

[0107] In combination with the detection results of Example 15, Example 18, Example 19, Comparative Example 3 and Comparative Example 4, it can be seen that the addition amount of the coating agent has a certain influence on the repellent effect of the repellent, and when the addition amount of the coating agent is 30-50 kg, the repellent effect of the repellent on the monochamus alternatus is best.

[0108] In combination with the detection results of Examples 20-22 and Examples 23-25, it can be seen that the repellent effect of Examples 23-25 is better than that of Examples 20-22, which indicates that the composition of the repellent is improved, and the added coating agent effectively improves the repellent effect of the repellent on the monochamus alternatus.

[0109] In combination with the detection results of Example 23, Example 26, Example 27, Comparative Example 5 and Comparative Example 6, it can be seen that the addition amount of the coating agent has a certain influence on the repellent effect of the repellent, and when the addition amount of the coating agent is 30-50 kg, the repellent effect of the repellent on the monochamus alternatus is best.

[0110] In combination with the detection results of Examples 28-30 and Examples 31-33, it can be seen that the repellent effect of Examples 31-33 is better than that of Examples 28-30, which indicates that the composition of the repellent is improved, and the added coating agent effectively improves the repellent effect of the repellent on the monochamus alternatus.

[0111] From the test results of Example 31, Example 34, Example 35, Comparative Example 7, and Comparative Example 8, it can be seen that the addition amount of the film-forming agent has certain influence on the repellent effect of the repellent, and when the addition amount of the film-forming agent is 30-50 kg, the repellent has the best repellent effect on the M. alternatus.

[0112] 2. In-forest repellent test 2.1. Selection of experimental area: Select a small plot of pine wood nematode disease epidemic, and divide the sample plot into circular areas to set multiple circular areas. 2.2. Treatment of repellent: Liquid repellent: The repellents prepared from Examples 1-19 and Comparative Examples 1-4 were dissolved with ethanol to prepare 10 L of repellent with a concentration of 0.6%.

[0113] Solid repellent: 20 kg of the repellents prepared from Examples 20-35 and Comparative Examples 5-8 were weighed.

[0114] 2.3. Experimental steps: The repellent and the M. alternatus trap were hung at the peak of M. alternatus emergence, and the specific steps were as follows: The repellent (liquid repellent or solid repellent) was hung at the center point of each circular area; In each circular area, the M. alternatus trap was set in the direction of the spread of the center point to the circumference of the circular area, with the repellent as the starting point, and the M. alternatus was attracted into the trap; the M. alternatus trap was provided with a matching M. alternatus attractant; the distance between the repellent and the M. alternatus trap was 20 meters.

[0115] The control group only hung the M. alternatus trap.

[0116] The hanging area was not affected by the repellent, and the number of M. alternatus in the trap was counted to evaluate the repellent effect of the M. alternatus, i.e., the protection effect.

[0117] Repellent effect (protection effect) calculation method: the number of M. alternatus trapped by the M. alternatus attractant near the repellent can be used to evaluate the field application effect of the repellent, and the calculation model is as follows: Wherein: CK is the number of adult insects trapped by the trap in the blank control group; Tr is the number of adult insects trapped by the trap in the treatment group; 2.4. The repellent effect was detected 15 days and 30 days after the repellent was applied, and the repellent rate detection results are shown in Table 2.

[0118] Table 2 repellency detection results According to the above table, the detection results of Examples 4-6 and Examples 10-12 show that the repellent prepared in Examples 4-6 has a significant decrease in repellent effect after 30 days, while the repellent prepared in Examples 10-12 has no change in repellent effect after 30 days, indicating that the added film-forming agent forms a dense protective layer on the outside of the drug, thereby effectively prolonging the repellent effect of the repellent on the Monochamus alternatus.

[0119] According to the detection results of Examples 10, 13, 14, Comparative Example 1 and Comparative Example 2, the amount of film-forming agent added has a certain effect on the effect of the repellent, and when the amount of film-forming agent added is 30-50 kg, the repellent has the best repellent effect on the Monochamus alternatus.

[0120] According to the detection results of Examples 7-9 and Examples 15-17, the repellent prepared in Examples 7-9 has a significant decrease in repellent effect after 30 days, while the repellent prepared in Examples 15-17 has no change in repellent effect after 30 days, indicating that the added film-forming agent forms a dense protective layer on the outside of the drug, thereby effectively prolonging the repellent effect of the repellent on the Monochamus alternatus.

[0121] According to the detection results of Examples 15, 18, 19, Comparative Example 3 and Comparative Example 4, the amount of film-forming agent added has a certain effect on the effect of the repellent, and when the amount of film-forming agent added is 30-50 kg, the repellent has the best repellent effect on the Monochamus alternatus.

[0122] According to the detection results of Examples 20-22 and Examples 23-25, the repellent prepared in Examples 20-22 has a significant decrease in repellent effect after 30 days, while the repellent prepared in Examples 23-25 has no change in repellent effect after 30 days, indicating that the added film-forming agent forms a dense protective layer on the outside of the drug, thereby effectively prolonging the repellent effect of the repellent on the Monochamus alternatus.

[0123] According to the detection results of Examples 23, 26, 27, Comparative Example 5 and Comparative Example 6, the amount of film-forming agent added has a certain effect on the effect of the repellent, and when the amount of film-forming agent added is 30-50 kg, the repellent has the best repellent effect on the Monochamus alternatus.

[0124] According to the detection results of Examples 28-30 and Examples 31-33, it can be seen that the repellent prepared in Examples 28-30 has a significant decrease in repellent effect after 30 days, while the repellent prepared in Examples 31-33 has no change in repellent effect after 30 days, indicating that the addition of the film-forming agent forms a dense protective layer on the outside of the drug, thereby effectively prolonging the repellent effect of the repellent on the Monochamus alternatus.

[0125] According to the detection results of Examples 31, Example 34, Example 35, Comparative Example 7, and Comparative Example 8, it can be seen that the amount of the film-forming agent added has a certain effect on the effect of the repellent, and when the amount of the film-forming agent added is 30-50 kg, the repellent has the best repellent effect on the Monochamus alternatus.

[0126] Example 36: In order to demonstrate the influence of the distance between the repellent and the Monochamus alternatus trap on the repellency rate, the distance between the repellent and the Monochamus alternatus trap was adjusted, and under the condition that other reaction conditions were unchanged, the influence of the distance between the repellent and the Monochamus alternatus trap on the repellency rate was detected, and the detection method was as follows: 3.1, selection of experimental area Select a small plot of pine wood nematode disease, and divide the sample plot into multiple circular areas. 3.2, treatment of repellent: The solid repellent prepared in Example 31 was used as the repellent of the present example, and the mass of the repellent was 20 kg.

[0127] The Monochamus alternatus trap is equipped with a matching Monochamus alternatus attractant.

[0128] 3.3, experimental steps: During the peak period of Monochamus alternatus emergence, the repellent and the Monochamus alternatus trap were hung at the intervals shown in Table 3, and the specific steps were as follows: The repellent was hung at the center point of each circular area; In each circular area, the Monochamus alternatus trap was set in the direction of the circular periphery of the circular area with the repellent set at the center point as the starting point, and the Monochamus alternatus was attracted into the trap.

[0129] The control group only hung the Monochamus alternatus trap.

[0130] The hanging area was not affected by the repellent, and the number of Monochamus alternatus in the trap was counted to evaluate the repellent effect of the repellent on the Monochamus alternatus, i.e., the protection effect.

[0131] The calculation method of the repellent effect (protection effect) is as follows: by measuring the number of Monochamus alternatus trapped by the Monochamus alternatus attractant near the repellent, the on-site application effect of the repellent can be evaluated, and the calculation model is as follows: CK is the number of adult insects trapped by the control group trap; Tr is the number of adult insects trapped by the treatment group trap; The repellency rate of the repellent was calculated based on the field records for 30 days, and the results are shown in Table 3.

[0132] Table 3 Effect of distance between the repellent and the M. alternatus trap on the repellency rate Group Suspension distance / m Repellency rate / % 1 0.5 100 2 0.5 100 3 0 36.1 0-10 100 36.2 10-20 100 36.3 20-30 79.55 36.4 30-40 72.73 36.5 40-50 47.06 36.6 50-60 18.5 According to Table 3, the repellency rate of the repellent at different distances shows a regular change, especially in the 0-10 m and 10-20 m hanging distances, the repellency rate of the M. alternatus reaches 100%, i.e. there is no M. alternatus adult activity in this range, which can achieve effective protection of healthy pine trees in this range. Considering the cost of the pesticide and the labor cost in use, the embodiment preferably selects 10-20 m as the best hanging distance of the repellent.

[0133] Example 37: When the repellent is hung on the pine trees in the forest, the dosage gradually decreases due to continuous volatilization, and when it is below a certain dosage, it will lose the repelling effect on the M. alternatus. In order to demonstrate the effect of the use time of the hung repellent on the repellency, the use time of the repellent was adjusted, and the effect of the use time of the repellent on the repellency rate was detected under the condition that other reaction conditions were unchanged, and the specific steps are as follows: 4.1, selection of experimental area Select a small plot of pine wood nematode disease, and divide the sample plot into circular areas to set multiple circular areas. 4.2, treatment of repellent: The solid repellent prepared in Example 31 was used as the repellent of this embodiment, and the mass of the repellent was 20 kg.

[0134] The M. alternatus trap is provided with a matching M. alternatus attractant.

[0135] 4.3, experimental steps: Hang the repellent and the M. alternatus trap at the peak of the M. alternatus emergence, as follows: Hang the repellent at the center point of each circular area; In each circular area, set the M. alternatus trap in the direction of the circular area spreading from the center point of the repellent set, and attract the M. alternatus into the trap; the distance between the repellent and the M. alternatus trap is 20 meters.

[0136] The control group only hung the M. alternatus trap.

[0137] The hanging area is not affected by the repellent, and the number of monochamus alternatus in the trap is counted to evaluate the repellent effect of the repellent on the beetle, i.e. the protection effect.

[0138] The calculation method of the repellent effect (protection effect) is as follows: by measuring the number of monochamus alternatus attracted by the attractant of monochamus alternatus near the repellent, the field application effect of the repellent can be evaluated, and the calculation model is as follows: Wherein: CK is the number of adult heads trapped in the blank control group trap; Tr is the number of adult heads trapped in the treatment group trap; The detection results of the repellent rate of the repellent used in the forest are shown in Table 4.

[0139] Table 4 Effect of use time of repellent on repellent rate According to Table 4, the repellent rate of the repellent at different hanging use times shows a regular change, especially within 15 days and 30 days after hanging, the repellent rate of the repellent to monochamus alternatus reaches 100%, which can realize effective protection of healthy pine trees within the time range, but after 45 days, the repellent rate of the repellent in the forest decreases greatly, and the protection effect decreases; considering the cost of the drug and the cost of labor in use, the application preferably selects 30 days as the best use time of the repellent.

[0140] In summary, the hanging distance between the repellent and the monochamus alternatus trap is 10-20 meters, the repellent is replaced every 30 days, the repellent effect of the repellent to monochamus alternatus reaches 100%, i.e. there is no monochamus alternatus adult activity in the range and time, which can realize more economical and effective protection of healthy pine trees.

Claims

1. A method of deploying a repellent effective to protect pine trees from infestation by pine wilt disease, the method comprising: The method comprises the following steps: ​ Dividing the deep forest into circular areas to set a plurality of circular areas; Setting a repellent at the center point of each circular area; In each circular area, setting a monochamus alternatus attractor in the direction of the center point of the circular area, with the repellent at the center point as the starting point, and attracting monochamus alternatus into the attractor; The distance between the repellent and the monochamus alternatus attractor is 0-40 meters; The monochamus alternatus attractor is provided with a monochamus alternatus attractant.

2. A method of deploying a repellent to effectively protect pine trees from infestation by pine wilt disease according to claim 1, characterized in that: The distance between the repellent and the monochamus alternatus attractor is 0-20 meters.

3. A method of deploying a repellent to effectively protect pine trees from infestation by pine wilt disease as defined in claim 1, wherein: The repellent is replaced regularly, and the steps are as follows: the repellent is replaced every 15-45 days; preferably, the interval is 15-30 days.

4. A method of deploying a repellent to protect pine trees from infestation by pine wilt disease according to any one of claims 1-3, characterized in that: The repellent comprises the following components by mass fraction: 4-allyl anisole 10-30 parts, beta-phenylacrylaldehyde 10-30 parts, ethanol 60-80 parts, Tween-80 2-8 parts, and linalool 10-20 parts.

5. A method of deploying a repellent to protect pine trees from infestation by pine wilt disease according to claim 4, characterized in that: The repellent further comprises eugenol 10-16 parts by mass.

6. A method of deploying a repellent to protect pine trees from infestation by pine wilt disease according to claim 4, characterized in that: The repellent further comprises benzyl acetate 5-15 parts by mass and vanillin 5-15 parts by mass.

7. A method of deploying a repellent to protect pine trees from infestation by pine wilt disease according to claim 5 or 6, characterized in that: The repellent further comprises a coating agent 30-50 parts by mass; the coating agent comprises the following components by mass fraction: gelatin 30-50 parts, quaternary ammonium chitosan 5-15 parts, glycerol 7-13 parts, linalool 1.6-2.5 parts, Tween-80 0.45-0.6 parts, and canmi starch 10-15 parts.

8. A method of deploying a repellent to protect pine trees from infestation by pine wilt disease according to claim 7, characterized in that: The repellent further comprises stearic acid 3.5-4.5 parts by mass and isopropyl myristate 2.5-3.5 parts by mass.

9. A method of deploying a repellent to protect pine trees from infestation by pine wilt disease according to claim 7, wherein: The preparation method of the coating agent comprises the following steps: Mixing gelatin and quaternary ammonium chitosan, adding glycerol for mixing, then adding the first part of linalool for mixing, adding Tween-80 for mixing to obtain a compound; Adding water to canmi starch for mixing, heating at 90-100°C for 1-2 hours, cooling to 20-30°C, adding the second part of linalool for mixing, heating to 30-50°C, and then adding the compound for mixing to obtain the coating agent.

10. A method of deploying a repellent to protect pine trees from infestation by pine wilt disease according to claim 9, wherein: The mass ratio of the first part of linalool to the second part of linalool is (1-2):(0.1-1.5).

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