Qingyuan partridge chicken sex identification method and application

Abstract

The invention discloses a Qingyuan partridge chicken sex identification method, which can flexibly select the identification mode and degree according to different requirements, such as different requirements on identification accuracy or different requirements on identification efficiency, is flexible to use, quantifies identification data and standards, does not depend on experience values of workers, and can be used for identifying the sex of Qingyuan partridge chickens. The method does not cause stress to the chicks and is suitable for large-scale rapid identification of the Qingyuan partridge chicks.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of chicken breeding, in particular to a method for identifying the gender of Qingyuan Game Chicken and application thereof. BACKGROUND

[0002] Qingyuan Game Chicken is originally from Qingyuan City, Guangdong Province, and the core production area specifically includes Qingcheng District, Qingxin District, Fogang County and Yingde City. It is named Qingyuan Game Chicken because the adult hens have small black spots on the back. In broiler production, different breeds have different needs for roosters and hens. In addition, consumers also have preferences for roosters or hens in terms of consumption. Therefore, it is necessary to identify the gender of chickens at one day of age. The earlier the gender of the chicken is determined, the more cost-effective the subsequent breeding and production will be. Gender identification is a long-standing challenge. At present, most of the gender identification of newly hatched chicks in China uses the anal flip technique. However, anal flip can cause stress and disease transmission in chicks, and improper operation can cause damage to the chicks. Moreover, the accuracy of anal flip identification depends entirely on the experience of workers, and it must be practiced for more than a month before anal flip operation can be performed. In addition, anal flip identification cannot be objectively and visually identified, and there are deficiencies in large-scale identification.

[0003] Currently, there is no method or standard for identifying the gender of Qingyuan Game Chicken by the pattern and pigment level of the plumage of newly hatched chicks. SUMMARY

[0004] The purpose of the present application is to provide a method for identifying the gender of Qingyuan Game Chicken and application thereof. The gender of Qingyuan Game Chicken newly hatched chicks is identified by the back plumage color, which can reduce the stress caused by anal flip identification on chicks, and overcome the dependence on the experience and subjective judgment of workers in anal flip identification. The method is more objective, efficient, flexible to use, and has high identification efficiency, making it easy to be widely used on a large scale.

[0005] According to a first aspect of the present application, a method for identifying the gender of Qingyuan Game Chicken is provided. The method identifies the gender of Qingyuan Game Chicken newly hatched chicks by judging the color of the dark band on the back of the chicks. Dark color indicates a female chick, and light color indicates a male chick. The dark band and the light band refer to the dark band and the light band on the back of Qingyuan Game Chicken newly hatched chicks. The light band refers to a 2-4 mm wide light yellow fluff band on both sides of the back of Qingyuan Game Chicken newly hatched chicks. The dark band refers to the dark fluff band in the middle of the two light bands on the back of Qingyuan Game Chicken newly hatched chicks and the dark fluff band on the other side of the light band. Thus, the method can quickly identify the gender of the chicks by observing the lightness and darkness of the dark band on the back of Qingyuan Game Chicken newly hatched chicks, and the accuracy is as high as 63% or more, making it suitable for large-scale rapid preliminary identification of the gender of chicks.

[0006] According to the second aspect of the present application, a method for identifying the gender of Qingyuan Game Chicken is provided, which comprises: if the gender of a newborn Qingyuan Game Chicken cannot be determined according to the color of the dark stripe, then determining the L* and b* values of the dark stripe of the chick, and the conditions for determining the gender of the newborn Qingyuan Game Chicken are: if the chick simultaneously satisfies the conditions that the L* value is within the range of 25.44-41.72 and the b* value is within the range of -5.26-23.48, then the chick is a male; and if the chick simultaneously satisfies the conditions that the L* value is within the range of 14.39-24.79 and the b* value is within the range of -6.66-15.12, then the chick is a female; wherein the L* value represents the brightness value of the feather, 0-100 represents the color from black to white, and the larger the value is, the brighter the color is, and the smaller the value is, the darker the color is; and the b* value represents the yellow value of the feather, a positive value indicates a yellowish color, and a negative value indicates a bluish color. Thus, if the color of the dark stripe of the newborn Qingyuan Game Chicken is not clear, and it is difficult to determine whether the color is dark or light, or if it is necessary to accurately determine the gender of a group of chicks, then the L* and b* values of the dark stripe can be determined to quantitatively determine the result, and the gender of the chick can be further determined efficiently and accurately.

[0007] According to the third aspect of the present application, a method for identifying the gender of Qingyuan Game Chicken is provided, which comprises: if the gender of a newborn Qingyuan Game Chicken cannot be determined according to the color of the dark stripe, and the L* and b* values of the dark stripe do not satisfy the conditions for determining the gender of the newborn Qingyuan Game Chicken (i.e., the gender of the chick cannot be determined by the color of the dark stripe and the L* and b* values of the dark stripe), then determining the L* and b* values of the light stripe of the chick, and the conditions for determining the gender of the newborn Qingyuan Game Chicken are: if the chick simultaneously satisfies the conditions that the L* value is within the range of 62.25-81.23 and the b* value is within the range of 11.68-28.25, then the chick is a male; and if the chick simultaneously satisfies the conditions that the L* value is within the range of 61.55-78.50 and the b* value is within the range of 14.90-30.36, then the chick is a female. Thus, if the gender of the chick cannot be determined by the color of the dark stripe and the L* and b* values of the dark stripe (i.e., the L* and b* values overlap or are not within the specified ranges, or the L* and b* values do not simultaneously satisfy the conditions), then the L* and b* values of the light stripe can be further determined to efficiently and accurately determine the gender of the chick.

[0008] According to the fourth aspect of the present application, a method for identifying the gender of Qingyuan Game Chicken is provided, which comprises: if the gender of a newborn Qingyuan Game Chicken cannot be determined according to the color of the dark stripe, the L* and b* values of the dark stripe do not satisfy the conditions for determining the gender of the newborn Qingyuan Game Chicken, and the L* and b* values of the light stripe also do not satisfy the conditions for determining the gender of the newborn Qingyuan Game Chicken (i.e., the gender of the chick cannot be determined by the color of the dark stripe, the L* and b* values of the dark stripe, and the L* and b* values of the light stripe), then determining the R 1-L* and R 1-b* values of the chick, and the conditions for determining the gender of the newborn Qingyuan Game Chicken are: if the chick simultaneously satisfies the conditions that the R1-L* value in the range of 1.49-3.19, R 1-b* value in the range of 1.20-2.22, then the chick is a male; if the chick satisfies the conditions of R 1-L* value in the range of 2.48-5.46, R 1-b* value in the range of 2-2.24, then the chick is a female; the R 1-L* value = absolute value of the ratio of the L* value of the light color band to the L* value of the dark color band, the R 1-b* value = absolute value of the ratio of the b* value of the light color band to the b* value of the dark color band. Thus, if the gender of the chick cannot be determined by the color of the dark color band, the L* and b* values of the dark color band, the L* and b* values of the light color band, the R 1-L* and R 1-b* values, the gender of the chick can be further determined more efficiently and accurately.

[0009] According to a fifth aspect of the present application, a method for identifying the gender of a Qingyuan chicken is provided, which comprises: if the gender of a newborn Qingyuan chicken cannot be determined by the color of the dark color band, the L* and b* values of the dark color band do not satisfy the conditions for determining the gender of a newborn Qingyuan chicken, the L* and b* values of the light color band do not satisfy the conditions for determining the gender of a newborn Qingyuan chicken, and the R 1-L* and R 1-b* values do not satisfy the conditions for determining the gender of a newborn Qingyuan chicken (i.e., the gender of the chick cannot be determined by the color of the dark color band, the L* and b* values of the dark color band, the L* and b* values of the light color band, the R 1-L* and R 1-b* values), the content of the melanin in the dark color band of the chick is further determined, and the conditions for determining the gender of a newborn Qingyuan chicken are: if the content of the melanin in the dark color band of the chick is in the range of 4.04-8.21 ng / g, then the chick is a male; if the content of the melanin in the dark color band of the chick is in the range of 3.48-6.75 ng / g, then the chick is a female. Thus, if the gender of the chick cannot be determined by the color of the dark color band, the L* and b* values of the dark color band, the L* and b* values of the light color band, the R 1-L* and R 1-b* values (the measured values overlap or are not in the specified range or do not satisfy the conditions at the same time), the content of the melanin in the dark color band can be further determined, and the gender of the chick can be determined more efficiently and accurately.

[0010] According to a sixth aspect of the present invention, a method for sex determination of Qingyuan Ma chickens is provided. The method includes: if the sex of newly hatched Qingyuan Ma chicken chicks cannot be determined based on the color of the dark band, and the L* and b* values ​​of the dark band do not meet the conditions for sex determination of newly hatched Qingyuan Ma chicken chicks, and the L* and b* values ​​of the light band also do not meet the conditions for sex determination of newly hatched Qingyuan Ma chicken chicks, and R... 1-L* and R 1-b* The values ​​also do not meet the conditions for determining the sex of newly hatched Qingyuan Ma chickens, and the content of melanin in the dark band also does not meet the conditions for determining the sex of newly hatched Qingyuan Ma chickens (i.e., through the color of the dark band, the L* and b* values ​​of the dark band, the L* and b* values ​​of the light band, and R...). 1-L* and R 1-b* If the sex of a chick cannot be determined by the color of the dark band or the content of pheomelanin in the dark band, then the eumelanin content in the dark band of the chick should be measured. The conditions for determining the sex of newly hatched Qingyuan Ma chicken chicks are: if the eumelanin content in the dark band is in the range of 3.72–6.15 ng / g, it is a male chick; if the eumelanin content in the dark band is in the range of 4.16–8.05 ng / g, it is a female chick. Therefore, if the sex of a chick is determined by the color of the dark band, the L* and b* values ​​of the dark band, the L* and b* values ​​of the light band, and the R... 1-L* and R 1-b* If the sex of chicks cannot be determined by the value of the dark band melanin content (when the measured values ​​overlap or are outside the specified range or do not meet the requirements at the same time), the eumelanin content of the dark band can be further measured to determine the sex of chicks more efficiently and accurately.

[0011] According to a seventh aspect of the present invention, a method for sex determination of Qingyuan Ma chickens is provided. The method includes: if the sex of newly hatched Qingyuan Ma chicken chicks cannot be determined based on the color of the dark band, and the L* and b* values ​​of the dark band do not meet the conditions for sex determination of newly hatched Qingyuan Ma chicken chicks, and the L* and b* values ​​of the light band also do not meet the conditions for sex determination of newly hatched Qingyuan Ma chicken chicks, and R... 1-L* and R 1-b* The values ​​also do not meet the conditions for determining the sex of newly hatched Qingyuan Ma chickens, and the content of pheomelanin in the dark band does not meet the conditions for determining the sex of newly hatched Qingyuan Ma chickens, nor does the content of eumelanin in the dark band meet the conditions for determining the sex of newly hatched Qingyuan Ma chickens (i.e., through the color of the dark band, the L* and b* values ​​of the dark band, the L* and b* values ​​of the light band, and R...). 1-L* and R 1-b*If the sex of a chick cannot be determined by the color of the dark band, the content of pheomelanin in the dark band, or the content of eumelanin in the dark band, then the pheomelanin content in the light band of the chick should be measured. The conditions for determining the sex of newly hatched Qingyuan Ma chicken chicks are: if the pheomelanin content in the light band is in the range of 6.54–8.74 ng / g, it is a male chick; if the pheomelanin content in the light band is in the range of 5.73–8.58 ng / g, it is a female chick. Therefore, if the sex of a chick cannot be determined by the color of the dark band, the L* and b* values ​​of the dark band, the L* and b* values ​​of the light band, and the R... 1-L* and R 1-b* If the sex of chicks cannot be determined by the value of pheomelanin in the dark band, the content of eumelanin in the dark band (when the measured values ​​overlap within the specified range, are outside the range, or do not meet the requirements simultaneously), then the content of pheomelanin in the light band can be further measured to determine the sex of chicks more efficiently and accurately.

[0012] According to an eighth aspect of the present invention, a method for sex determination of Qingyuan Ma chickens is provided. The method includes: if the sex of newly hatched Qingyuan Ma chicken chicks cannot be determined based on the color of the dark band, and the L* and b* values ​​of the dark band do not meet the conditions for sex determination of newly hatched Qingyuan Ma chicken chicks, and the L* and b* values ​​of the light band also do not meet the conditions for sex determination of newly hatched Qingyuan Ma chicken chicks, and R... 1-L* and R 1-b* The values ​​also do not meet the conditions for determining the sex of newly hatched Qingyuan Ma chickens, and the content of pheomelanin in the dark band does not meet the conditions for determining the sex of newly hatched Qingyuan Ma chickens, nor does the content of eumelanin in the dark band meet the conditions for determining the sex of newly hatched Qingyuan Ma chickens, nor does the content of pheomelanin in the light band meet the conditions for determining the sex of newly hatched Qingyuan Ma chickens (i.e., by the color of the dark band, the L* and b* values ​​of the dark band, the L* and b* values ​​of the light band, and R...). 1-L* and R 1-b* If the sex of a chick cannot be determined by the color of the dark band, the content of pheomelanin in the dark band, the content of eumelanin in the dark band, or the content of pheomelanin in the light band, then the eumelanin content in the light band of the chick should be measured. The conditions for determining the sex of newly hatched Qingyuan Ma chicken chicks are: if the eumelanin content in the light band is in the range of 2.44–5.18 ng / g, it is a male chick; if the eumelanin content in the light band is in the range of 3.01–5.57 ng / g, it is a female chick. Therefore, if the sex of a chick cannot be determined by the color of the dark band, the L* and b* values ​​of the dark band, the L* and b* values ​​of the light band, and the R... 1-L* and R 1-b* If the sex of chicks cannot be determined by the values ​​of pheomelanin in the dark band, eumelanin in the dark band, and pheomelanin in the light band (when the measured values ​​overlap within the specified range, are outside the range, or do not meet the requirements simultaneously), then the eumelanin content in the light band can be further measured to determine the sex of chicks more efficiently and accurately.

[0013] According to a ninth aspect of the present invention, a method for sex determination of Qingyuan Ma chickens is provided. The method includes: if the sex of newly hatched Qingyuan Ma chicken chicks cannot be determined based on the color of the dark band, and the L* and b* values ​​of the dark band do not meet the conditions for sex determination of newly hatched Qingyuan Ma chicken chicks, and the L* and b* values ​​of the light band also do not meet the conditions for sex determination of newly hatched Qingyuan Ma chicken chicks, and R... 1-L* and R 1-b* The values ​​also do not meet the conditions for determining the sex of newly hatched Qingyuan Ma chickens, and the content of pheomelanin in the dark band does not meet the conditions for determining the sex of newly hatched Qingyuan Ma chickens, nor does the content of eumelanin in the dark band meet the conditions for determining the sex of newly hatched Qingyuan Ma chickens, nor does the content of pheomelanin in the light band meet the conditions for determining the sex of newly hatched Qingyuan Ma chickens (i.e., by the color of the dark band, the L* and b* values ​​of the dark band, the L* and b* values ​​of the light band, and R...). 1-L* and R 1-b* If the values ​​of pheomelanin content in the dark band, eumelanin content in the dark band, pheomelanin content in the light band, and eumelanin content in the light band cannot determine the sex of the chick, then the ratio R of the eumelanin content in the light band to the dark band of the chick should be measured. 3-e* If the chick R 3-e* If the value is in the range of 0.4 to 0.790, then it is a male chick; if the chick R 3-e* If the value is in the range of 0.791 to 1.39, it is a female chick; the R value... 3-e* Value = Eumelanin content of the light-colored band / Eumelanin content of the dark-colored band. Therefore, if we consider the color of the dark-colored band, its L* and b* values, and the L* and b* values ​​of the light-colored band, R... 1-L* and R 1-b* If the sex of chicks cannot be determined by the values ​​of pheomelanin content in the dark band, eumelanin content in the dark band, pheomelanin content in the light band, or eumelanin content in the light band (when the measured values ​​overlap within the specified range, are outside the range, or do not meet the requirements simultaneously), then the ratio R of eumelanin content in the light band to the dark band can be further measured. 3-e* This allows for more efficient and accurate determination of the sex of chicks.

[0014] According to a tenth aspect of the present invention, the application of the above-described method in sex determination of Qingyuan Ma chicken chicks is provided. Through this application, different levels of sex determination methods can be selected according to actual conditions and needs, and the accuracy rate can reach 63.49-95.23%.

[0015] The beneficial effects of this invention are:

[0016] The application discloses a method for identifying the gender of Qingyuan Game chickens, which can flexibly select the identification mode and degree according to different needs, such as different requirements for identification accuracy or different requirements for identification efficiency, is flexible in use, quantifies identification data and standards, does not depend on the experience value of workers, does not cause stress to the chicks, and is suitable for large-scale and rapid identification of Qingyuan Game chicken chicks. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 Fig. 1 is a schematic diagram of the positions of frog lines of Qingyuan Game chicken chicks, wherein the arrows indicate the positions of the frog lines;

[0018] Figure 2 Fig. 2 is a schematic diagram of the positions of dark bands and light bands of Qingyuan Game chicken chicks. DETAILED DESCRIPTION

[0019] The application will be further described in detail below with reference to the drawings.

[0020] The frog lines of the newly-born Qingyuan Game chicken chicks refer to two clear light yellow down bands with a width of 2-4 mm on both sides of the back feathers, which are called frog lines, as shown in the arrows in Fig. 1. Figure 1 The light yellow down bands at the positions of the frog lines are called light bands, and the dark down bands in the middle of the two light bands and the dark down bands on the other sides of the light bands are called dark bands, as shown in Fig. 2. Figure 2 The dark bands and the light bands are collectively called frog lines.

[0021] Example 1: The gender of the newly-born Qingyuan Game chicken chicks is determined by comparing the colors of the dark bands.

[0022] Randomly selected 1-day-old Qingyuan Game chickens from different batches are observed, and a total of 4 batches of 2000 chickens are randomly observed. The colors of the dark bands are observed, and the dark bands are divided into a "dark group" (as shown in the right side of Fig. 1) and a "light group" (as shown in the left side of Fig. 1). Figure 1 The "dark group" is preliminarily determined as female chicks, and the "light group" is preliminarily determined as male chicks. Figure 1

[0023] The genders of the chicks in the above groups are identified by rectal examination, and the data of each group are sorted, and the female chick rate of the "dark group" and the male chick rate of the "light group" are counted.

[0024] The experimental results are shown in Table 1.

[0025] Table 1: Average accuracy rate of feather color identification gender

[0026]

[0027] Note: Dark back feathers are female chicks, and light back feathers are male chicks.

[0028] ​Table 1 shows that the sex of newly hatched Qingyuan Ma chickens can be determined directly by the color of the dark band on the frog-like markings. A darker band indicates a female chick, while a lighter band indicates a male chick. Comparing this to the results of vent examination, the accuracy of sex determination by feather color can reach 70% to 75%. Table 1 establishes the following sex determination criteria for newly hatched Qingyuan Ma chickens: a darker band on the markings indicates a female chick, while a lighter band indicates a male chick.

[0029] Example 2: The sex of newly hatched Qingyuan Ma chicken chicks was determined by the color difference value of the markings.

[0030] Five hundred one-day-old Qingyuan Ma chicks with their sex determined were randomly selected, and the color values ​​of the feathers at the spotted areas were measured.

[0031] The feather color of the dark and light bands on the back of 1-day-old chicks was measured three times, and the average value was taken.

[0032] Feather color parameters were calculated using a CR-400 colorimeter (the quantification parameters of the colorimeter are shown in Table 2). The brightness value (L*), redness value (a*), and yellowness value (b*) were obtained, and the average value was taken as the feather color parameters of the chicken to be tested to evaluate the similarity or difference of the colors.

[0033] Table 2 Quantitative parameters of colorimeter

[0034]

[0035] Note: 0 in a* and b* represents no color difference.

[0036] As shown in Table 2: the brightness value L* represents the brightness of the feathers, with 0-100 representing black to white. The larger the value, the brighter the feathers, and the smaller the value, the darker the feathers. The yellowness value b* represents the ratio of yellow to blue in the feathers, with positive values ​​indicating a yellowish tint and negative values ​​indicating a bluish tint. The redness value a* represents the ratio of red to green in the feathers, with positive values ​​indicating a reddish tint and negative values ​​indicating a greenish tint.

[0037] The feather color of the dark and light bands on the back markings of 1-day-old chicks of known sex was measured and analyzed using feather color quantification parameters. The results are shown in Table 3.

[0038] Table 3. Overall Characteristics Analysis of Feather Color Quantitative Parameters for 1-Day-Old Qingyuan Ma Chicken Chicks

[0039]

[0040] Note: The same lowercase letter indicates no significant difference (P>0.05) between different feather colors and sex groups; different lowercase letters indicate significant differences (P<0.05). R1 ​​represents the ratio of each parameter of the light to dark bands within the same sex; R2 represents the ratio of each parameter of the dark (or light) bands between different sexes.

[0041] From Table 3, it can be seen that the chroma values of the dark and light bands of the male and female chicks were determined by a colorimeter, and it was found that the brightness L* value and the yellowness b* value of the male and female chicks were significantly different (P<0.05). The average values of the L* value of the dark and light bands of the female and male chicks were 19.20, 34.26, 68.64, and 75.41, respectively, and the average values of the b* value were 3.70, 11.53, 22.52, and 22.91, respectively. The L* value and the b* value had the same trend, i.e., the smaller the value, the darker the color, and the trend of the a* value was opposite. Therefore, the difference between the dark and light bands of the same gender was mainly reflected in the difference in the b* value and the L* value, and the b* value and the L* value of the female chicks were greater than those of the male chicks, and the color difference was more obvious. The comparison between different genders focused on the dark band, and the a* value was not significantly different as the priority determination parameter. According to the analysis, it was found that the smaller the brightness L* value and the yellowness b* value, the darker the color, and the larger the redness a* value, the darker the color.

[0042] And from Table 2, it can be seen that the further judgment standard for the gender of the Qingyuan chicken chicks at birth (1 day old) is:

[0043] 1. First, determine the L* and b* values of the dark band of the Qingyuan chicken chicks at birth, and take the simultaneous compliance of the L* and b* values as the main determination parameter. The conditions for determining the gender of the Qingyuan chicken chicks at birth are as follows: if the chick simultaneously satisfies the L* value in the range of 25.44-41.72 and the b* value in the range of -5.26-23.48, it is a male chick; if the chick simultaneously satisfies the L* value in the range of 14.39-24.79 and the b* value in the range of -6.66-15.12, it is a female chick; if the measured value overlaps within the specified range or is not within the range or does not simultaneously satisfy, then determine the L* and b* values of the light band again.

[0044] 2. Then, determine the L* and b* values of the light band of the chick at birth again, and take the simultaneous compliance of the L* and b* values as the main determination parameter. The conditions for determining the gender of the Qingyuan chicken chicks at birth are as follows: if the chick simultaneously satisfies the L* value in the range of 62.25-81.23 and the b* value in the range of 11.68-28.25, it is a male chick; if the chick simultaneously satisfies the L* value in the range of 61.55-78.50 and the b* value in the range of 14.90-30.36, it is a female chick; if the measured value overlaps within the specified range or is not within the range or does not simultaneously satisfy, then determine the ratio R 1-L* and R 1-b* (R 1-L* = L* value of light band / L* value of dark band, R 1-b* = b* value of light band / b* value of dark band, and take the absolute value) of the light band and the dark band again.

[0045] 3. Then, determine R 1-L* and R 1-b*The condition for judging the gender of the newborn chicks of Qingyuan Gamecock is that if the chick satisfies R 1-L* value is in the range of 1.49-3.19, R 1-b* value is in the range of 1.20-2.22, it is a male chick; if the chick satisfies R 1-L* value is in the range of 2.48-5.46, R 1-b* value is in the range of 2-2.24, it is a female chick.

[0046] Example 3: judging the gender of the newborn chicks of Qingyuan Gamecock by pigment content.

[0047] The enzyme-linked reaction analysis method (ELISA) is used to determine the sample, and the double-antibody sandwich method kit (Shanghai Enzyme-linked Company-Shanghai Yujing YJ40023B) is used to determine the levels of eumelanin and phaeomelanin in the sample. The determination method includes the following steps:

[0048] (1) prepare reagents, samples and standards;

[0049] (2) add the prepared samples and standards, and react at 37°C for 30 minutes;

[0050] (3) wash the plate 5 times, add enzyme-labeled reagent, and react at 37°C for 30 minutes;

[0051] (4) wash the plate 5 times, add color developing liquid A and B, and develop color at 37°C for 10 minutes;

[0052] (5) add the termination liquid;

[0053] (6) read the OD value within 15 minutes;

[0054] (7) calculate the pigment content.

[0055] The determination results of different pigments in the back pattern are shown in Table 4:

[0056] Table 4: back feather eumelanin content (ng / g) of 1-day-old chicks of Qingyuan Gamecock

[0057]

[0058] Note: different feather colors and gender groups with the same lowercase letters represent no significant difference; different lowercase letters represent significant difference (P<0.05), R3 represents the ratio of each pigment in the light color band and the dark color band in the same gender; R4 represents the ratio of each pigment in the dark color band (or light color band) between different genders.

[0059] From the results in Table 4: melanin in male and female groups and within the group were no significant difference (P>0.05); eumelanin in male and female groups and within the group were no significant difference (P<0.05); pheomelanin in male and female groups and within the group were no significant difference (P<0.05).

[0060] Accordingly, in the case of not being able to identify the gender of the chicks by feather color (color difference), the gender of the Qingyuan Game chicken can be further determined by the content of pigments, mainly pheomelanin and eumelanin, specifically:

[0061] 1. Determining the content of pheomelanin in the dark band of the Qingyuan Game chicken, the conditions for determining the gender of the Qingyuan Game chicken are: if the content of pheomelanin in the dark band is within the range of 4.04-8.21 ng / g, it is a male chick; if the content of pheomelanin in the dark band is within the range of 3.48-6.75 ng / g, it is a female chick; if the measured value overlaps within the specified range or is not within the range or does not meet the conditions at the same time, then the content of eumelanin in the dark band is measured again.

[0062] 2. Determining the content of eumelanin in the dark band of the Qingyuan Game chicken, the conditions for determining the gender of the Qingyuan Game chicken are: if the content of eumelanin in the dark band is within the range of 3.72-6.15 ng / g, it is a male chick; if the content of eumelanin in the dark band is within the range of 4.16-8.05 ng / g, it is a female chick; if the measured value overlaps within the specified range or is not within the range or does not meet the conditions at the same time, then the content of pigment in the light band is measured again.

[0063] 3. Determining the content of pheomelanin in the light band of the Qingyuan Game chicken, the conditions for determining the gender of the Qingyuan Game chicken are: if the content of pheomelanin in the light band is within the range of 6.54-8.74 ng / g, it is a male chick; if the content of pheomelanin in the light band is within the range of 5.73-8.58 ng / g, it is a female chick; if the measured value overlaps within the specified range or is not within the range or does not meet the conditions at the same time, then the content of eumelanin in the light band is measured again.

[0064] 4. Determining the content of eumelanin in the light band of the Qingyuan Game chicken, the conditions for determining the gender of the Qingyuan Game chicken are: if the content of eumelanin in the light band is within the range of 2.44-5.18 ng / g, it is a male chick; if the content of eumelanin in the light band is within the range of 3.01-5.57 ng / g, it is a female chick; if the measured value overlaps within the specified range or is not within the range or does not meet the conditions at the same time, then the ratio R of the content of eumelanin in the light band to the content of eumelanin in the dark band is measured again. 3-e* R 3-e* value = content of eumelanin in the light band / content of eumelanin in the dark band;

[0065] 5. Determine the eumelanin content of the light and dark bands in newly hatched Qingyuan Ma chickens and calculate the ratio R. 3-e* The condition for determining the sex of newly hatched Qingyuan Ma chicken chicks is: if the chick is R... 3-e* If the value is in the range of 0.4 to 0.790, then it is a male chick; if the chick R 3-e* If the value is in the range of 0.791 to 1.39, it is a female chick.

[0066] Combining Examples 1-3, a method for determining the sex of newly hatched Qingyuan Ma chicken chicks is obtained:

[0067] S1. For newly hatched Qingyuan Ma chickens, the darker bands on the markings indicate female chicks, while the lighter bands indicate male chicks. If the color difference is not obvious, the L* and b* values ​​of the darker bands should be measured.

[0068] S2. Next, measure the L* and b* values ​​of the dark band of the newly hatched chick. The main judgment parameter is that the L* and b* values ​​must be met simultaneously. If the chick meets the condition that the L* value is in the range of 25.44 to 41.72 and the b* value is in the range of -5.26 to 23.48, it is a male chick; if the chick meets the condition that the L* value is in the range of 14.39 to 24.79 and the b* value is in the range of -6.66 to 15.12, it is a female chick; if the measured values ​​overlap within the specified range, are not within the range, or do not meet the condition simultaneously, then measure the L* and b* values ​​of the light band.

[0069] S3. Next, measure the L* and b* values ​​of the light-colored band of the newly hatched chick. The primary judgment parameter is that both L* and b* values ​​must meet the specified criteria simultaneously. If the chick's L* value is within the range of 62.25–81.23 and its b* value is within the range of 11.68–28.25, it is a male chick; if its L* value is within the range of 61.55–78.50 and its b* value is within the range of 14.90–30.36, it is a female chick. If the measured values ​​overlap within the specified range, are outside the range, or do not meet the criteria simultaneously, then measure the ratio R of the light-colored band to the dark-colored band. 1-L* and R 1-b* (R 1-L* = Light band L* value / Dark band L* value; R 1-b* = b* value of light band / b* value of dark band (take the absolute value of the result);

[0070] S4. Further measure the R of the newly hatched chick. 1-L* and R 1-b* Value: in R 1-L* and R 1-b* The value simultaneously meets the main judgment parameter. If the chick simultaneously satisfies R... 1-L* Values ​​range from 1.49 to 3.19, R 1-b* If the value is in the range of 1.20 to 2.22, then it is a male chick; if the chick also satisfies R... 1-L* Values ​​range from 2.48 to 5.46, R1-b* If the value is in the range of 2-2.24, it is a female; if the value overlaps within the specified range or is not within the range, or if it does not meet the requirement at the same time, then re-determine the pigment content in the dark and light band feathers;

[0071] S5, re-determine the dark band melanin content of the newly hatched chicks: if the dark band melanin content of the chicks is in the range of 4.04-8.21 ng / g, it is a male; if the dark band melanin content of the chicks is in the range of 3.48-6.75 ng / g, it is a female; if the value overlaps within the specified range or is not within the range, then re-determine the eumelanin content in the dark band;

[0072] S6, re-determine the dark band eumelanin content of the newly hatched chicks: if the dark band eumelanin content of the chicks is in the range of 3.72-6.15 ng / g, it is a male; if the dark band eumelanin content of the chicks is in the range of 4.16-8.05 ng / g, it is a female; if the value overlaps within the specified range or is not within the range, then re-determine the pheomelanin content in the light band;

[0073] S7, re-determine the pheomelanin content in the light band of the newly hatched chicks: if the pheomelanin content in the light band of the chicks is in the range of 6.54-8.74 ng / g, it is a male; if the pheomelanin content in the light band of the chicks is in the range of 5.73-8.58 ng / g, it is a female; if the value overlaps within the specified range or is not within the range, then re-determine the eumelanin in the light band;

[0074] S8, re-determine the eumelanin content in the light band of the newly hatched chicks: if the eumelanin content in the light band of the chicks is in the range of 2.44-5.18 ng / g, it is a male; if the eumelanin content in the light band of the chicks is in the range of 3.01-5.57 ng / g, it is a female; if the value overlaps within the specified range or is not within the range, then re-determine the ratio R 3-e* of eumelanin content in the light band to eumelanin content in the dark band;

[0075] S9, re-determine the ratio R 3-e* of eumelanin content in the light band to eumelanin content in the dark band: if the R 3-e* value of the chicks is in the range of 0.4-0.790, it is a male; if the R 3-e* value of the chicks is in the range of 0.791-1.39, it is a female.

[0076] The specific judgment standard data is shown in Tables 5 and 6:

[0077] Table 5 Judgment standard table of Qingyuan chicken newly hatched chicks (determination value)

[0078]

[0079]

[0080] Table 6 Standard table for judging the gender of Qingyuan Game Chicken newly hatched chicks (ratio)

[0081] [R 1-L* values]] [R 1-b* values]] [R 3-e* values]] Gender determination 1.49～3.19 1.20～2.22 0.4～0.790 Male chicks 2.48～5.46 2～2.24 0.791～1.39 Female chicks

[0082] Example 4 Application of the gender identification of Qingyuan Game Chicken newly hatched chicks.

[0083] Select 126 one-day-old Qingyuan Game Chicken chicks for gender identification: first, divide the chicks into a dark group of 45, a light group of 37, and an uncertain group of 44 according to the color of the dark band (the uncertain group is not clear in color and cannot be determined as dark or light); the dark group is 45 female chicks, and the light group is 37 male chicks; then measure the L* and b* values of the dark band of the 44 chicks in the uncertain group, and compare them with the standard table in Table 5 to identify 14 male chicks, 12 female chicks, and 18 uncertain chicks (the uncertain group is when the measured value overlaps within the specified range or is not within the range or at the same time, the same below); then measure the L* and b* values of the light band of the 18 chicks in the uncertain group, and compare them with the standard table in Table 5 to identify 2 male chicks, 3 female chicks, and 13 uncertain chicks; then measure the R 1-L* and R 1-b* values of the 13 chicks in the uncertain group, and compare them with the standard table in Table 6 to identify 2 male chicks, 1 female chick, and 10 uncertain chicks; then measure the melanin content of the dark band of the 10 chicks in the uncertain group, and compare it with the standard table in Table 5 to identify 1 male chick, 2 female chicks, and 7 uncertain chicks; then measure the eumelanin content of the dark band of the 7 chicks in the uncertain group, and compare it with the standard table in Table 5 to identify 2 male chicks, 1 female chick, and 4 uncertain chicks; then measure the melanin content of the light band of the 4 chicks in the uncertain group, and compare it with the standard table in Table 5 to identify 1 male chick, 1 female chick, and 2 uncertain chicks; then measure the eumelanin content of the light band of the 2 chicks in the uncertain group, and compare it with the standard table in Table 5 to identify 1 male chick, 0 female chicks, and 1 uncertain chick; then measure the ratio of the eumelanin content of the light band to the dark band of the 1 chick in the uncertain group, and compare it with the standard table in Table 6 to identify 0 male chicks, 1 female chick, and 0 uncertain chicks. Then determine the accurate gender of the 126 chicks by rectal examination, and then compare the identification results with the accurate gender, and the statistical results are shown in Table 7: 3-e*

[0084] Table 7 Accuracy rate detection results table of the method for judging the gender of Qingyuan Game Chicken newly hatched chicks

[0085] Identification method S1 S2 S3 S4 S5 S6 S7 S8 S9 Number of identifications 82 26 5 3 3 3 2 1 1 Number of correct identifications 80 25 4 3 3 2 1 1 1 Accuracy (%) 63.49 83.33 86.51 88.89 91.26 92.86 93.65 94.44 95.23

[0086] Note:

[0087] ​1. The table S1-9 represents the S1-9 conditions in the method for determining the gender of Qingyuan Game Chicken, and the specific conditions are as follows: S1, determining by naked eye observation of the color depth of the dark band, S2, determining by detecting the L* and b* values of the dark band, S3, determining by detecting the L* and b* values of the light band, S4, determining by detecting the R 1-L* and R 1-b* values, S5, determining by detecting the eumelanin content value of the dark band, S6, determining by detecting the eumelanin content value of the dark band, S7, determining by detecting the eumelanin content value of the light band, S8, determining by detecting the eumelanin content value of the light band, and S9, determining by detecting the R 3-e* value of the eumelanin content ratio of the light band to the dark band.

[0088] 2. The total accuracy rate = (T1+T2+T3+...+Tn) / 126*100%, wherein Tn represents the number of correct genders in the Sn condition, and for example, the calculation method of 86.51% is (80+25+4) / 126*100%.

[0089] The results in Table 7 show that the accuracy rate of determining the gender of Qingyuan Game Chicken only by the color depth of the dark band is 63.49% (the identification result is lower than that in Table 1, because the gender of all the chicken groups is determined in Table 1, but the uncertain group is separated in Table 7 and is regarded as incorrect data, and the accuracy rate of 63.49% = 80 / 126*100% is calculated, so the accuracy rate is lower than that in Table 1; and if the identification accuracy rate is calculated only from the number of identifications and the number of correct identifications, it is 80 / 82*100% = 97.56%), if the L* and b* values of the dark band of the uncertain group are determined and judged, the accuracy rate is 83.33%, if the L* and b* values of the light band of the uncertain group are further determined and judged, the accuracy rate is 86.51%, if the R 1-L* and R 1-b* values of the uncertain group are further determined and judged, the accuracy rate is 88.89%, if the eumelanin content value of the dark band of the uncertain group is further determined and judged, the accuracy rate is 91.26%, if the eumelanin content value of the dark band of the uncertain group is further determined and judged, the accuracy rate is 92.86%, if the eumelanin content value of the light band of the uncertain group is further determined and judged, the accuracy rate is 93.65%, if the eumelanin content value of the light band of the uncertain group is further determined and judged, the accuracy rate is 94.44%, and if the R 3-e* value of the eumelanin content ratio of the light band to the dark band of the uncertain group is further determined and judged, the accuracy rate is 95.23%.

[0090] Through gender identification of 126 chicks, 120 chicks were finally identified, and the accuracy rate reached 95.23%. As shown in Table 7, the step-by-step identification method in this scheme can adapt to various application scenarios. When large-scale chicks are identified, only the color depth of the dark band can be determined to preliminarily identify the gender of the chicks, and the accuracy rate can reach more than 63.49%. If it is necessary to further improve the accuracy of gender identification, the L* and b* values of the dark band can be measured and gender identification can be performed according to the standard, and the accuracy rate can reach 83.33%. If it is necessary to further improve the accuracy of gender identification, the L* and b* values of the light band can be measured and gender identification can be performed according to the standard, and the accuracy rate can reach more than 86.51%. If it is necessary to further improve the accuracy of gender identification, the R 1-L* and R 1-b* values can be measured and gender identification can be performed according to the standard, and the accuracy rate can reach more than 88.89%. If it is necessary to further improve the accuracy of gender identification, the melanin content of the dark band can be measured and gender identification can be performed according to the standard, and the accuracy rate can reach more than 91.26%. If it is necessary to further improve the accuracy of gender identification, the eumelanin content of the dark band can be measured and gender identification can be performed according to the standard, and the accuracy rate can reach more than 92.86%. If it is necessary to further improve the accuracy of gender identification, the melanin content of the light band can be measured and gender identification can be performed according to the standard, and the accuracy rate can reach more than 93.65%. If it is necessary to further improve the accuracy of gender identification, the eumelanin content of the light band can be measured and gender identification can be performed according to the standard, and the accuracy rate can reach more than 94.44%. If it is necessary to further improve the accuracy of gender identification, the ratio R 3-e* of the eumelanin contents of the light band and the dark band can be measured and gender identification can be performed according to the standard, and the accuracy rate can reach more than 95.23%.

[0091] For example, when large-scale chicks are identified, the color of the dark band of the chicks can be used to preliminarily identify the gender of the chicks, and the chick population can be divided into a male chick group, a female chick group, and an uncertain group. Then, the L* and b* values of the dark band of all groups or only the uncertain group can be measured to further identify the gender of the chicks, and the chick population can be divided into a male chick group, a female chick group, and an uncertain group again. Then, the L* and b* values of the light band of the uncertain group can be measured to identify the gender of the chicks, and the chick population can be divided into a male chick group, a female chick group, and an uncertain group again. Then, the R 1-L* and R 1-b*Sexing is determined by measuring the amount of pheomelanin in the dark band of the chicks, which then further divides the chicks into male, female, and undetermined groups. If necessary, the pheomelanin content in the dark band of the undetermined group can be measured for sexing, and the chicks can be further divided into male, female, and undetermined groups. If necessary, the eumelanin content in the dark band of the undetermined group can be measured for sexing, and the chicks can be further divided into male, female, and undetermined groups. If necessary, the pheomelanin content in the light band of the undetermined group can be measured for sexing, and the chicks can be further divided into male, female, and undetermined groups. If necessary, the eumelanin content in the light band of the undetermined group can be measured for sexing, and the chicks can be further divided into male, female, and undetermined groups. Finally, the ratio R of the eumelanin content in the light band to the dark band of the undetermined group can be measured. 3-e* Sex determination was performed to further divide the chicks into male chicks, female chicks, and an undetermined group.

[0092] Therefore, when conducting large-scale sex determination of chicks, depending on the required accuracy, the option to continue testing or discontinue testing for uncertain groups can be used flexibly to meet different application scenarios. Furthermore, feather color determination and pigment content determination do not rely on human experience and can be performed in large batches. Compared to traditional vent examination for sex determination, this method not only avoids stressing chicks but is also more efficient, more flexible, and has better applicability.

Claims

1. A method for sexing Qingyuan chicken, wherein, The method judges the color of the dark band on the back of the newborn Qiyuan chicken, and the dark color indicates a female chick, and the light color indicates a male chick; the back of the newborn Qiyuan chicken has a dark band and a light band, the light band refers to a light yellow fluff band with a width of 2-4 mm on both sides of the back of the newborn Qiyuan chicken, and the dark band refers to a dark fluff band between the two light bands on the back of the newborn Qiyuan chicken.

2. A method for sexing Qingyuan chicken, wherein, The method comprises: if the color of the dark band in claim 1 cannot be used to judge the gender of the newborn Qiyuan chicken, then the L* and b* values of the dark band of the chick are measured again, and the conditions for judging the gender of the newborn Qiyuan chicken are that if the chick simultaneously satisfies the L* value in the range of 25.44-41.72 and the b* value in the range of -5.26-23.48, then the chick is a male; and if the chick simultaneously satisfies the L* value in the range of 14.39-24.79 and the b* value in the range of -6.66-15.12, then the chick is a female; the L* value represents the brightness value of the feather, and the b* value represents the yellow value of the feather.

3. A method for sexing Qingyuan chicken, wherein, The method comprises: if the color of the dark band in claim 1 cannot be used to judge the gender of the newborn Qiyuan chicken, and the L* and b* values of the dark band do not satisfy the conditions for judging the gender of the newborn Qiyuan chicken in claim 2, then the L* and b* values of the light band of the chick are measured again, and the conditions for judging the gender of the newborn Qiyuan chicken are that if the chick simultaneously satisfies the L* value in the range of 62.25-81.23 and the b* value in the range of 11.68-28.25, then the chick is a male; and if the chick simultaneously satisfies the L* value in the range of 61.55-78.50 and the b* value in the range of 14.90-30.36, then the chick is a female.

4. A method for sexing Qingyuan chicken, wherein, The method comprises: if the color of the dark band according to claim 1 cannot be used to determine the gender of the Qingyuan Game chicken newborn chick, and the L* and b* values of the dark band do not satisfy the conditions for determining the gender of the Qingyuan Game chicken newborn chick according to claim 2, and the L* and b* values of the light band also do not satisfy the conditions for determining the gender of the Qingyuan Game chicken newborn chick according to claim 3, then the R 1-L* and R 1-b* values of the chick are determined again, and the conditions for determining the gender of the Qingyuan Game chicken newborn chick are as follows: if the chick simultaneously satisfies the R 1-L* value being in the range of 1.49-3.19 and the R 1-b* value being in the range of 1.20-2.22, then it is a male chick; if the chick simultaneously satisfies the R 1-L* value being in the range of 2.48-5.46 and the R 1-b* value being in the range of 2-2.24, then it is a female chick; the R 1-L* value = the L* value of the light band / L* value of the dark band, the R 1-b* value = the b* value of the light band / b* value of the dark band, and the result is an absolute value.

5. A method for sexing Qingyuan chicken, wherein, The method includes: if the dark band color as described in claim 1 cannot determine the sex of newly hatched Qingyuan Ma chicken chicks, and the L* and b* values ​​of the dark band do not meet the conditions for determining the sex of newly hatched Qingyuan Ma chicken chicks as described in claim 2, and the L* and b* values ​​of the light band also do not meet the conditions for determining the sex of newly hatched Qingyuan Ma chicken chicks as described in claim 3, and R 1-L* and R 1-b* If the value does not meet the conditions for determining the sex of newly hatched Qingyuan Ma chicken chicks as described in claim 4, then the content of dark brown melanin in the chick shall be measured again. The conditions for determining the sex of newly hatched Qingyuan Ma chicken chicks are as follows: if the content of dark brown melanin in the chick is in the range of 4.04 to 8.21 ng / g, then it is a male chick; if the content of dark brown melanin in the chick is in the range of 3.48 to 6.75 ng / g, then it is a female chick.

6. A method for sexing Qingyuan chicken, wherein, The method comprises: if the color of the dark band according to claim 1 cannot be used to determine the gender of the Qingyuan Game chicken newborn chick, and the L* and b* values of the dark band do not satisfy the conditions for determining the gender of the Qingyuan Game chicken newborn chick according to claim 2, and the L* and b* values of the light band do not satisfy the conditions for determining the gender of the Qingyuan Game chicken newborn chick according to claim 3, and the R 1-L* and R 1-b* values do not satisfy the conditions for determining the gender of the Qingyuan Game chicken newborn chick according to claim 4, and the eumelanin content of the dark band does not satisfy the conditions for determining the gender of the Qingyuan Game chicken newborn chick according to claim 5, then the eumelanin content of the dark band of the chick is determined again, and the conditions for determining the gender of the Qingyuan Game chicken newborn chick are that if the eumelanin content of the dark band of the chick is in the range of 3.72-6.15 ng / g, then the chick is a male; and if the eumelanin content of the dark band of the chick is in the range of 4.16-8.05 ng / g, then the chick is a female.

7. A method for sexing Qingyuan chicken, wherein, The method includes: if the dark band color as described in claim 1 cannot determine the sex of newly hatched Qingyuan Ma chicken chicks, and the L* and b* values ​​of the dark band do not meet the conditions for determining the sex of newly hatched Qingyuan Ma chicken chicks as described in claim 2, and the L* and b* values ​​of the light band also do not meet the conditions for determining the sex of newly hatched Qingyuan Ma chicken chicks as described in claim 3, and R 1-L* and R 1-b* The values ​​also do not meet the conditions for determining the sex of newly hatched Qingyuan Ma chicken chicks as described in claim 4, and the content of pheomelanin in the dark band does not meet the conditions for determining the sex of newly hatched Qingyuan Ma chicken chicks as described in claim 5, and the content of eumelanin in the dark band does not meet the conditions for determining the sex of newly hatched Qingyuan Ma chicken chicks as described in claim 6. Therefore, the content of pheomelanin in the light band of the chick is measured again. The conditions for determining the sex of newly hatched Qingyuan Ma chicken chicks are: if the content of pheomelanin in the light band of the chick is in the range of 6.54–8.74 ng / g, it is a male chick; if the content of pheomelanin in the light band of the chick is in the range of 5.73–8.58 ng / g, it is a female chick.

8. A method for sexing Qingyuan chicken, wherein, The method includes: if the dark band color as described in claim 1 cannot determine the sex of newly hatched Qingyuan Ma chicken chicks, and the L* and b* values ​​of the dark band do not meet the conditions for determining the sex of newly hatched Qingyuan Ma chicken chicks as described in claim 2, and the L* and b* values ​​of the light band also do not meet the conditions for determining the sex of newly hatched Qingyuan Ma chicken chicks as described in claim 3, and R 1-L* and R 1-b* The values ​​also do not meet the conditions for determining the sex of newly hatched Qingyuan Ma chickens as described in claim 4, and the content of pheomelanin in the dark band does not meet the conditions for determining the sex of newly hatched Qingyuan Ma chickens as described in claim 5, and the content of eumelanin in the dark band does not meet the conditions for determining the sex of newly hatched Qingyuan Ma chickens as described in claim 6, and the content of pheomelanin in the light band does not meet the conditions for determining the sex of newly hatched Qingyuan Ma chickens as described in claim 7. Therefore, the content of eumelanin in the light band of the chick is measured again. The conditions for determining the sex of newly hatched Qingyuan Ma chickens are: if the content of eumelanin in the light band of the chick is in the range of 2.44–5.18 ng / g, it is a male chick; if the content of eumelanin in the light band of the chick is in the range of 3.01–5.57 ng / g, it is a female chick.

9. A method for sexing Qingyuan chicken, wherein, The method comprises: if the color of the dark band according to claim 1 cannot be used to determine the gender of the Qingyuan Game chicken newborn chick, and the L* and b* values of the dark band do not satisfy the conditions for determining the gender of the Qingyuan Game chicken newborn chick according to claim 2, and the L* and b* values of the light band do not satisfy the conditions for determining the gender of the Qingyuan Game chicken newborn chick according to claim 3, and the R 1-L* and b* values do not satisfy the conditions for determining the gender of the Qingyuan Game chicken newborn chick according to claim 4, and the melanin content of the dark band does not satisfy the conditions for determining the gender of the Qingyuan Game chicken newborn chick according to claim 5, and the eumelanin content of the dark band does not satisfy the conditions for determining the gender of the Qingyuan Game chicken newborn chick according to claim 6, and the melanin content of the light band does not satisfy the conditions for determining the gender of the Qingyuan Game chicken newborn chick according to claim 7, and the eumelanin content of the light band does not satisfy the conditions for determining the gender of the Qingyuan Game chicken newborn chick according to claim 8, then the eumelanin content ratio R 3-e* of the light band and the dark band of the chick is determined again. 3-e* The conditions for determining the gender of the Qingyuan Game chicken newborn chick are: if the R 3-e* value of the chick is within the range of 0.4-0.790, then the chick is a male; if the R 3-e* value of the chick is within the range of 0.791-1.39, then the chick is a female; and the R The method judges the color of the dark band on the back of the newborn Qiyuan chicken, and the dark color indicates a female chick, and the light color indicates a male chick; the back of the newborn Qiyuan chicken has a dark band and a light band, the light band refers to a light yellow fluff band with a width of 2-4 mm on both sides of the back of the newborn Qiyuan chicken, and the dark band refers to a dark fluff band between the two light bands on the back of the newborn Qiyuan chicken. value = eumelanin content value of the light band / eumelanin content value of the dark band.

10. The method in any one of claims 1-9 is applied to the gender identification of the newborn Qiyuan chicken.

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