Composite bag for prolonging preservation time of germplasm resources and preparation method

Abstract

The invention discloses a composite bag for prolonging the preservation time of germplasm resources and a preparation method thereof, and relates to the technical field of preservation of crop genetic resources, the key points of the technical scheme are as follows: S1, preparation of a bag A: taking malonic acid, performing dehydration treatment, and then loading the dried malonic acid into a material bag with good air permeability; s2, preparing a bag B: dehydrating a carbon dioxide slow-release agent, and then loading the dried carbon dioxide slow-release agent into a material bag with good air permeability; s3, preparing a bag C: putting the insecticide matched with an auxiliary material into a material bag with good air permeability, and packaging; and S4, sealing and packaging: sequentially putting the bag A, the bag B and the bag C into a polyethylene preservative film bag. The method can effectively prevent invasion of plant diseases and insect pests, and realizes long-term preservation of resources on the premise of not damaging physiological activity and hereditary stability of germplasm resources.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of the preservation of crop genetic resources, and in particular to a composite package for prolonging the preservation time of germplasm resources and a preparation method thereof. BACKGROUND

[0002] With the rapid development of biotechnology, the preservation technology of crop germplasm resources is particularly important. Crop germplasm resources are not only the source of new varieties, but also the key to maintaining biological diversity. Traditional germplasm resource preservation methods such as refrigeration and drying have problems such as loss of active ingredients, dehydration damage, and pest attacks, making it difficult to achieve long-term effective preservation.

[0003] Currently, pests and diseases are a major problem affecting the preservation of crop germplasm resources. As shown in "Crop Determination Pest Stay", pests and diseases not only cause damage to crops growing in the field, but also pose a threat to germplasm resources during storage. In addition, the preservation environment of germplasm subjects also has a significant impact on their physiological activity and genetic stability. As described in "Principle and Technology of Safe Preservation of Crop Germplasm Resources" published by Lu Xinxiu, mastering the preservation principles and technologies of germplasm resources can more effectively prolong the preservation time of germplasm resources and maintain their genetic integrity. Traditional preservation techniques and existing pest control methods cannot simultaneously address the two issues of prolonging preservation time and controlling pests. In particular, as mentioned in "Analysis of Research Trends in Crop Germplasm Resources" published by Zhang Aimin, China's research level in germplasm resources occupies a certain position in the world, but still needs to break through in handling technical problems in this field.

[0004] Therefore, there is an urgent need to develop a new crop germplasm resource preservation technology that can effectively prevent pest attacks and achieve long-term preservation of resources without compromising the physiological activity and genetic stability of germplasm resources. In view of this, the present application provides a composite package for prolonging the preservation time of germplasm resources and a preparation method thereof. SUMMARY

[0005] The purpose of the present application is to provide a composite package for prolonging the preservation time of germplasm resources and a preparation method thereof, as well as a preparation method, to solve the above problems.

[0006] The above technical purpose of the present application is achieved by the following technical solution:

[0007] The present application provides a composite package for prolonging the preservation time of germplasm resources and a preparation method thereof, S1. Configuration A package: take malonic acid, dehydrate, then put the dried malonic acid into a material bag with good air permeability;

[0008] S2. Configuration B package: dehydrate the carbon dioxide release agent, then put the dried carbon dioxide release agent into a material bag with good air permeability;

[0009] S3. Configuration C package: pesticide auxiliary material is matched and packed in a material bag with good air permeability;

[0010] S4. Sealed package: A package, B package and C package are sequentially placed in a polyethylene fresh-keeping film bag.

[0011] With reference to the first aspect, the application further provides that the dehydration treatment is vacuum drying.

[0012] With reference to the first aspect, the application further provides that the carbon dioxide release agent is a tartaric acid capable of releasing carbon dioxide.

[0013] With reference to the first aspect, the application further provides that the pesticide is Spinetoram.

[0014] With reference to the first aspect, the application further provides that the auxiliary material is castor oil.

[0015] The second aspect of the application also provides a composite package for prolonging the storage time of germplasm resources, characterized by being prepared by any of the above methods.

[0016] In summary, the application has the following beneficial effects: the application realizes precise and continuous environmental control of crop germplasm resources, and maximally protects the genetic integrity and vitality of the germplasm resources. Secondly, the composite package is convenient for large-scale production and use, is low in cost and easy to use, and is suitable for germplasm resource preservation of different scales. Finally, the safe and non-toxic characteristics of the composite package ensure food safety of the germplasm resources in the long-term storage process, and provide an important guarantee for the reasonable development and utilization of germplasm resources in the future. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 is a flow chart of a preparation method of a composite package for prolonging the storage time of germplasm resources in the embodiments of the application. DETAILED DESCRIPTION

[0018] The technical solutions in the embodiments of the application will be clearly and completely described below with reference to the drawings in the embodiments of the application. Obviously, the described embodiments are only part of the embodiments of the application, rather than all the embodiments of the application. Based on the embodiments in the application, all other embodiments obtained by a person of ordinary skill in the art without creative work fall within the protection scope of the application.

[0019] Embodiment 1:

[0020] A preparation method of a composite package for prolonging the storage time of germplasm resources, as shown in Figure 1 the figure, comprises the following steps:

[0021] Preparation of A package: Take malonic acid in an appropriate amount, and dehydrate it by vacuum drying or other methods. Then, put the dried malonic acid into a material bag with good air permeability. The size and volume ratio of the bag are set so that the malonic acid in the bag can fully play a deoxidizing role. The material bag with good air permeability can be a non-woven bag.

[0022] Preparation of B package: Carbon dioxide release agent, such as tartaric acid, is obtained by similar processing methods to obtain dry substances and is packed in a non-woven bag. The tartaric acid not only can further control the oxygen concentration in the package environment, but also can generate corresponding carbon dioxide through chemical reaction when the deoxidizer is insufficient, maintaining a low-oxygen environment. The material bag with good air permeability can be a non-woven bag.

[0023] Preparation of C package: Select an ethofenprox that has passed safety certification on the market as an insecticide, and combine it with certain auxiliary materials such as castor oil to enable the ethofenprox to be continuously released in the bag, achieving long-term insecticidal or insect repellent effect, and then use a non-woven bag to package it.

[0024] After the preparation of the above three components, they are sequentially placed in a polyethylene (PE) fresh-keeping film bag, and a certain distance is maintained between the packages to prevent mutual interaction and affect the effect. In addition, the fresh-keeping film bag needs to be strictly sealed to ensure its safety and preservation effect during storage and transportation.

[0025] Example 2:

[0026] The composite package prepared by the method of Example 1.

[0027] When storing crop seeds, the composite package is mixed with the seeds in a certain proportion. Generally, 1-2 composite packages are used per kilogram of seeds, and the amount used is adjusted according to actual conditions. During storage, the survival rate and vitality of the seeds should be regularly tested to determine the actual effect of the composite package and whether a new composite package needs to be replaced to maintain a low-oxygen and low-pest environment.

[0028] Experimental example:

[0029] The composite package provided by the present application is used for verification in a storage environment full of pests. First, adjust the temperature and humidity in the storage space to suitable conditions for storing crop seeds. Then, spread a certain amount of ethofenprox in the storage space to reduce the initial interference of pests on the seeds.

[0030] Secondly, the seeds and the composite package are mixed in a ratio of 1:2 and stored in a polyethylene (PE) fresh-keeping film bag, and ensure that the A package, B package and C package are evenly distributed around the seeds, so that the seeds can be in a low-oxygen and low-pest environment during the entire storage process.

[0031] During the subsequent storage period, the environmental conditions are monitored by periodically detecting the oxygen content and the number of pests in the bag, and the number of composite bags is adjusted or the composite bags are replaced according to the results. Throughout the experiment, the team records the shelf life, seed germination rate and other related data, analyzes the protection effect of the composite bag, and optimizes the scheme.

[0032] After three months of testing, compared with the traditional storage method, the composite bag of the application can significantly improve the survival rate and vitality of the seeds, and significantly reduce the number of pests, verifying the effectiveness and practical value of the composite bag in practical application.

[0033] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and not for limiting the present application, although the foregoing embodiments of the present application are described in detail, for those skilled in the art, it still can be modified, or part of the technical features of the equivalent replacement of the technical solutions recorded in the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. within the spirit and principles of the present application shall be included within the scope of protection of the present application.

Claims

1. A method for preparing a composite package to extend the preservation time of germplasm resources, characterized in that: It comprises the following steps: S1. Configuration A bag: take malonic acid, and carry out dehydration treatment, and then put the dried malonic acid into a material bag with good air permeability; S2. Configuration B bag: carry out dehydration treatment on the carbon dioxide slow-release agent, and then put the dried carbon dioxide slow-release agent into a material bag with good air permeability; S3. Configuration C bag: put the pesticide and auxiliary materials together, and seal them in a material bag with good air permeability; S4. Seal the package: put the A bag, B bag and C bag into a polyethylene fresh-keeping film bag in sequence.

2. The method for preparing a composite package for prolonging the storage time of germplasm resources according to claim 1, characterized in that: The dehydration treatment is vacuum drying.

3. The method for preparing a composite package for prolonging the storage time of germplasm resources according to claim 1, characterized in that: The carbon dioxide slow-release agent is a tartaric acid capable of slowly releasing carbon dioxide.

4. The method for preparing a composite package for prolonging the storage time of germplasm resources according to claim 1, characterized in that: The pesticide is spirodiclofen.

5. The method for preparing a composite package for prolonging the storage time of germplasm resources according to claim 1, characterized in that: The auxiliary material is castor oil.

6. The method for preparing a composite package for prolonging the storage time of germplasm resources according to claim 1, characterized in that: The material bag with good air permeability is a non-woven fabric bag.

7. A composite package for extending the shelf life of germplasm, characterized by: It is prepared by the method of any one of claims 1-6.

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