Mycobacterium tuberculosis collection and pretreatment device based on excrement specimen

Abstract

The invention belongs to the field of mycobacterium tuberculosis detection, and particularly relates to a mycobacterium tuberculosis collection and pretreatment device based on excrement specimens, the mycobacterium tuberculosis collection and pretreatment device comprises a sampling tube, a sealing cover and a collection tube, the two ends of the sealing cover are provided with internal threads, and the collection tube and the sampling tube are in threaded connection with the upper end and the lower end of the sealing cover respectively; a sealing piston is slidably arranged in the collecting pipe, an air exhaust hole is formed in the bottom of the collecting pipe, a liquid storage cavity is formed in the side, located on the sampling pipe, of the sealing cover, a first rubber film layer is arranged on the inner wall of the liquid storage cavity in a surrounding mode, specimen treatment liquid is contained in the liquid storage cavity, and a second rubber film layer is sealed at the bottom of the liquid storage cavity. A plurality of air holes penetrate through the surface of the sealing cover, and the sampling tube is provided with cutting bulges corresponding to the second rubber film layer. According to the invention, collection and pretreatment of mycobacterium tuberculosis based on excrement specimens can be realized, qualified specimens are provided for subsequent related laboratory detection, and sample liquid can be prevented from leaking and polluting operators and the environment.

Description

TECHNICAL FIELD

[0001] The application belongs to the field of Mycobacterium tuberculosis detection, and particularly relates to a Mycobacterium tuberculosis collecting and pretreatment device based on a fecal sample. BACKGROUND

[0002] Tuberculosis is one of the most deadly infectious diseases in the world caused by Mycobacterium tuberculosis infection.

[0003] Traditional tuberculosis laboratory pathogen detection is mainly based on sputum samples, however, it is difficult for the elderly, children, patients with severe illness and patients with extrapulmonary tuberculosis to cough up sputum or to cough up sputum meeting the requirements, and fewer patients can provide sputum samples at the end of treatment, so the test method relying on sputum samples as tuberculosis pathogen detection has great limitations, which can easily lead to delayed diagnosis and missed diagnosis. In recent years, studies have shown that Mycobacterium tuberculosis can be detected in the feces of tuberculosis patients after the patients are infected with Mycobacterium tuberculosis, therefore, laboratory detection methods based on fecal samples have emerged.

[0004] However, the traditional fecal collector only has a test tube and a sealing cover, and the feces need to be transferred multiple times during the collection and processing process, which can cause sample droplets to leak and contaminate the operator, and the through hole also has the problem of residual liquid flowing out when the test container is withdrawn, which can easily cause the operator to feel uncomfortable in taste and sense.

[0005] Therefore, how to provide a Mycobacterium tuberculosis collecting and pretreatment device based on a fecal sample, which has good sealing performance and can avoid odor diffusion and environmental pollution, has become a problem that needs to be considered by those skilled in the art. SUMMARY

[0006] In view of the problems mentioned in the background, the application discloses a Mycobacterium tuberculosis collecting and pretreatment device based on a fecal sample, which can avoid odor diffusion and environmental pollution by pretreating the feces in a closed environment. The application specifically discloses the following technical scheme:

[0007] A Mycobacterium tuberculosis collecting and pretreatment device based on a fecal sample, comprising a sampling tube, a sealing cover and a collecting tube, both ends of the sealing cover are provided with internal threads, the collecting tube and the sampling tube are threadedly connected to the upper and lower ends of the sealing cover respectively, a sealing piston is slidably arranged in the collecting tube, the bottom of the collecting tube is provided with an air extraction hole, the sealing cover located on one side of the sampling tube is provided with a liquid storage cavity, a first rubber film layer is arranged around the inner wall of the liquid storage cavity, the liquid storage cavity is filled with a sample treatment liquid, the bottom of the liquid storage cavity is sealed with a second rubber film layer, a plurality of air holes are formed through the surface of the sealing cover, and a cutting protrusion is arranged on the sampling tube corresponding to the second rubber film layer.

[0008] Further, the bottom of the liquid storage cavity is provided with an annular protrusion near the side of the sampling tube, the lower surface edge of the annular protrusion is provided with an annular sealing groove, and the inner side of the annular sealing groove is provided with a cutting groove corresponding to the cutting protrusion, and the unfolded edge of the second rubber film layer is fixed in the annular sealing groove.

[0009] Further, the top of the liquid storage cavity is provided with a hollow fixing frame, the hollow fixing frame is provided with a sampling spoon, the back of the hollow fixing frame is provided with a collection funnel, the edge of the collection funnel is sealed with the edge of the sealing cover, and the collection funnel is provided with a biological film layer.

[0010] Further, the front end of the sealing piston is provided with a dot-shaped protrusion, and the dot-shaped protrusion is inserted into the liquid outlet of the collection funnel.

[0011] Further, the middle part of the first rubber film layer is fixed on the sampling spoon.

[0012] Further, a plurality of mixed beads are further included, and the mixed beads are arranged in the sampling tube.

[0013] Further, a primary filter screen is further included, and the primary filter screen is arranged on the hollow fixing frame and used for filtering and blocking the mixed beads.

[0014] Further, the top end of the sampling tube and the collection tube is provided with a sealing washer corresponding to the threaded part of the sealing cover.

[0015] Further, a limiting washer is further included, and the limiting washer is sleeved at the bottom end of the threaded joint of the sampling tube.

[0016] Further, an aluminum foil tin paper layer is further included, and the aluminum foil tin paper layer is packaged on the air extraction hole and seals the air extraction hole before air extraction.

[0017] The present application has the following advantages: the present application realizes the whole-process closed operation of the pretreatment of Mycobacterium tuberculosis through the integrated sealing of the sampling tube, the sealing cover and the collection tube, and the sealing cover does not need to be opened during operation, thereby reducing the risk of sample exposure and leakage, ensuring the safety of operation and the cleanliness of the environment, avoiding the discomfort of the taste and senses of the operator, avoiding the risk of sample contamination since the cover does not need to be opened during the pretreatment process, and improving the sensitivity and accuracy of detection. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 It is a schematic diagram of the main body of the present application;

[0019] Figure 2 It is a schematic diagram of the top structure of the sampling tube of the present application;

[0020] Figure 3 It is a sectional view of the present application;

[0021] Figure 4 for Figure 3 Enlarged view of a portion of point A in the middle;

[0022] Figure 5 This is a perspective sectional view of the sealing cap of the present invention;

[0023] Figure 6 This is a schematic diagram showing the arrangement of the hollow fixing frame and the primary filter screen in the sealing cover of the present invention;

[0024] Figure 7 This is a front view of the annular protrusion in the sealing cap of the present invention;

[0025] Figure 8 This is a three-dimensional sectional view of the collection tube of the present invention.

[0026] Among them, 1-sampling tube; 2-sealing cap; 3-collection tube; 4-sealing gasket; 5-mixing bead; 6-first rubber film layer; 7-second rubber film layer; 8-vent hole; 9-cutting protrusion; 10-sealing piston; 11-extraction hole; 12-hollow fixing frame; 13-sampling spoon; 14-biofilm layer; 15-collection funnel; 16-limiting gasket; 17-cutting groove; 18-primary filter screen; 19-annular protrusion. Detailed Implementation

[0027] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0028] Example 1

[0029] like Figures 1-8 As shown, this embodiment discloses a simple, disposable fecal specimen-based Mycobacterium tuberculosis collection and pretreatment device for medical personnel. It includes a sampling tube 1, a sealing cap 2, and a collection tube 3. Both ends of the sealing cap 2 are provided with internal threads. Sealing gaskets 4 for leak prevention are installed at the threaded connections of both ends of the sealing cap 2 and the collection tube 3. The collection tube 3 is threaded to the top of the sealing cap 2, and the sampling tube 1 is threaded to the bottom of the sealing cap 2. Two mixing beads 5 are placed inside the sampling tube 1 to generate turbulence through oscillation or compression when the sample and treatment solution are mixed, accelerating the full contact between the treatment solution and the sample and ensuring complete dissolution of the fecal specimen.

[0030] The sealing cover 2 in the embodiment is located at one side of the sampling tube 1 and is provided with a liquid storage cavity. The top of the liquid storage cavity is provided with a hollow fixing frame 12. The center of the hollow fixing frame 12 is provided with a sampling spoon 13. The sampling spoon 13 is used for extracting the fecal sample. The hollow part of the hollow fixing frame 12 is provided with a primary filter screen 18. The primary filter screen 18 is used for filtering large-particle impurities and blocking the mixed beads 5. The back of the hollow fixing frame 12 is provided with a collecting funnel 15. The collecting funnel 15 is used for collecting the mixed liquid. The edge of the collecting funnel 15 is sealed with the edge of the sealing cover 2. The collecting funnel 15 is provided with a biological membrane layer 14. The biological membrane layer 14 is used for filtering and separating the treatment liquid. The biological membrane layer in the embodiment is a biological filter membrane. Nitrocellulose membrane is specifically used. The bottom of the liquid storage cavity is provided with an annular protrusion 17 near one side of the sampling tube 1. The lower surface edge of the annular protrusion 17 is provided with an annular sealing groove. The width of the annular sealing groove is the same as the width of the sealing gasket. The inner wall of the liquid storage cavity is provided with a first rubber film layer 6. Specifically, the top of the first rubber film layer 6 is sealingly connected to the collecting funnel 15. The bottom of the first rubber film layer 6 is sealingly connected to the annular protrusion 17. The liquid storage cavity is filled with a specimen treatment liquid. The bottom of the liquid storage cavity is sealingly provided with a second rubber film layer 7. Specifically, the unfolded edge of the second rubber film layer 7 is fixed in the annular sealing groove. The middle of the second rubber film layer 7 is fixed to the sampling spoon 13. The surface of the sealing cover 2 is provided with a plurality of air holes 8. The air holes 8 can balance the internal and external air pressure during the air extraction process. The sampling tube 1 is injection molded with a cutting protrusion 9 with a height of 2 mm corresponding to the second rubber film layer 7.

[0031] As a preferred embodiment of the application, the limiting gasket 16 is also included. The height of the limiting gasket 16 is slightly higher than that of the cutting protrusion 9. The limiting gasket 16 is sleeved at the bottom end of the threaded joint of the sampling tube 1. The limiting gasket 16 is used to avoid the direct contact between the cutting protrusion 9 and the second rubber film layer 7. After the sealing cover 2 is unscrewed from the sampling tube 1 before sampling, the limiting gasket 16 is removed.

[0032] As a preferred embodiment of the application, the inner side of the annular sealing groove of the sealing cover 2 is provided with a cutting groove 19 corresponding to the cutting protrusion 9. The edge of the second rubber film layer 7 covers the cutting groove 19. The cutting protrusion 9 can accurately pierce the film. The treatment liquid leakage or insufficient release caused by the deviation of the piercing position is prevented. When the cutting protrusion 9 contacts the second rubber film layer 7, the sealing gasket 4 at the top of the sampling tube 1 enters the annular sealing groove. As the cutting protrusion 9 enters the cutting groove 19 to cut the second rubber film layer 7, the sealing gasket 4 is stretched and deformed after entering the annular sealing groove. The sampling tube and the sealing cover are effectively sealed. The liquid spilling and odor diffusion are prevented.

[0033] The inside of the collecting tube 3 in the embodiment is slidably installed with a sealing piston 10, the front end of the sealing piston 10 is provided with a dot-shaped protrusion, the dot-shaped protrusion is inserted into the liquid outlet of the collecting funnel 15, the bottom of the collecting tube 3 is provided with a suction hole 11, the suction hole 11 is used for connecting a suction pump, and the collecting tube is used for collecting the sample treatment liquid after filtration and separation.

[0034] As a preferred embodiment of the present application, an aluminum foil tin paper layer is further included, the aluminum foil tin paper layer is packaged on the suction hole 11, and the suction hole 11 is blocked before suction.

[0035] The sampling mode of the present application is as follows:

[0036] S01. The sampling tube 1 is taken off from the sealing cover 2, and the limiting gasket 16 is removed, the sampling spoon 13 on the sealing cover 2 is used to sample the fecal specimen, and the general fixed amount of digging of the feces is 0.8-1.0g;

[0037] S02. The sealing cover 2 is reassembled on the sampling tube 1, the cutting protrusion 9 is used to cut the second rubber film layer 7 by screwing, the second rubber film layer 7 after cutting is shrunk and fixed on the sampling spoon 13, and the specimen treatment liquid in the liquid storage cavity enters the sampling tube 1 and is mixed with the fecal specimen in the sampling tube 1.

[0038] S03. The sampling tube 1 is shaken, the mixed beads 5 are used to accelerate the dissolution of the fecal specimen in the treatment liquid, and then the room temperature is placed.

[0039] S04. The aluminum foil tin paper layer is torn off, the suction pump is connected with the suction hole 11 of the collecting tube 3, the treatment device is turned over, the suction pump is started, with the deformation of the first rubber film layer 6 under negative pressure, the treatment liquid in the liquid storage cavity is filtered through the primary filter screen 18 and the biological membrane layer 14 and is sucked into the collecting tube 3, and the collection of the sample is completed.

[0040] The above is only a preferred embodiment of the present application, and does not limit the technical scope of the present application, so any slight modification, equivalent change and modification of the above embodiment according to the technical essence of the present application still belongs to the scope of the technical solution of the present application.

Claims

1. A device for collection and pre-treatment of Mycobacterium tuberculosis based on a fecal sample, characterized in that, Including sampling tube, sealing cover and collection tube, both ends of the sealing cover are provided with internal threads, the collection tube and the sampling tube are respectively threadedly connected at the upper and lower ends of the sealing cover, a sealing piston is slidably arranged in the inside of the collection tube, the bottom of the collection tube is provided with a gas extraction hole, the sealing cover located on one side of the sampling tube is provided with a liquid storage cavity, a first rubber film layer is arranged on the inner wall of the liquid storage cavity, the liquid storage cavity is filled with specimen processing liquid, the bottom of the liquid storage cavity is sealed with a second rubber film layer, a plurality of air holes are formed through the surface of the sealing cover, and the sampling tube is provided with a cutting protrusion corresponding to the second rubber film layer.

2. A device for collecting and pre-treating Mycobacterium tuberculosis based on fecal samples according to claim 1, characterized in that, An annular protrusion is arranged at the bottom of the liquid storage cavity close to the side of the sampling tube, an annular sealing groove is arranged at the lower surface edge of the annular protrusion, a cutting groove is arranged in the inner side of the annular sealing groove corresponding to the cutting protrusion, and the second rubber film layer is fixed in the annular sealing groove after being unfolded.

3. A device for collecting and pre-treating Mycobacterium tuberculosis based on fecal specimen according to claim 1, characterized in that, The top of the liquid storage cavity is provided with a hollow fixing frame, a sampling spoon is installed on the hollow fixing frame, a collection funnel is arranged at the back of the hollow fixing frame, the edge of the collection funnel is sealed with the edge of the sealing cover, and a biological membrane layer is arranged in the collection funnel.

4. A device for collecting and pre-treating Mycobacterium tuberculosis based on fecal samples according to claim 3, characterized in that, A point-shaped protrusion is arranged at the front end of the sealing piston, and the point-shaped protrusion is inserted into the liquid outlet of the collection funnel.

5. A device for collecting and pre-treating Mycobacterium tuberculosis based on fecal samples according to claim 3, characterized in that, The middle part of the first rubber film layer is fixed on the sampling spoon.

6. A device for collecting and pre-treating Mycobacterium tuberculosis based on fecal samples according to claim 3, characterized in that, A plurality of mixed beads are further arranged in the sampling tube.

7. A device for collecting and pre-treating Mycobacterium tuberculosis based on fecal samples according to claim 6, characterized in that, A primary filter screen is further arranged on the hollow fixing frame and used for filtering and blocking the mixed beads.

8. A device for collecting and pre-treating Mycobacterium tuberculosis based on fecal samples according to claim 1, characterized in that, Sealing washers are installed at the top ends of the sampling tube and the collection tube corresponding to the threaded part of the sealing cover.

9. A device for collecting and pre-treating Mycobacterium tuberculosis based on fecal samples according to claim 1, characterized in that, A limiting washer is further arranged around the threaded joint of the sampling tube.

10. The device for collecting and pre-treating Mycobacterium tuberculosis based on fecal specimen according to claim 1, characterized in that, An aluminum foil tin paper layer is further arranged and encapsulated on the gas extraction hole to block the gas extraction hole before gas extraction.

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